Spectrafuge™ 24D Microcentrifuge
Contents
1. EQUIPMENT DISPOSAL This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed of with unsorted waste Instead it s your responsibility to correctly dispose of your equipment at lifecycle end by handing it over to an authorized facility for separate collection and recycling It s also you responsibility to decontaminate the equipment in case oof biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling of the equipment For more information about where you can drop off your waste equipment please contact your local dealer from whom you originally purchased this equipment By doing so you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health Thank you Labnet International Inc Labnet 31 Mayfield Ave Edison NJ 08837 P 732 417 0700 F 732 417 1750 Labnet International Inc Email labnetinfo corning com www labnetinternational com 10 Declaration of Conformity Number CE 00013 Manufacturer Labnet International Inc 31 Mayfield Ave Edison NJ 08837 USA Labnet International declares that the devices described below are in conformity with the EC directives listed In the event of unauthorized modification of any of the devices listed2. WARNING Do not attempt to open the lid of any centrifuge until the rotor has come to a complete stop In the event of a power failure or malfunction it may be necessary to open the lid manually 1 Disconnect the power cord from the wall socket 2 Remove the plastic plug located on the left side of the unit 3 Pull the wire attached to the plug to open the lid lock manually 4 4 Lid lock The centrifuge can be started only with the lid securely closed When a run is started the lid lock automatically activates Do not attempt to open the lid during a centrifuge run At the end of the run the lid will automatically unlock Never attempt to override the lid lock mechanism Doing so is dangerous and could damage the centrifuge 4 5 Speed selection see Figure 2 The speed rpm or g force can be selected from 500 to 13 300 rpm in 100 rpm increments or from 100 to 16 300 x g with the knob on the right g force or rpm display is set by pressing the knob Turn the knob to increase or decrease the value 4 6 Selection of operating time momentary operation Start Stop Operating time can be selected from 0 5 min to 30 min by turning the timer knob on the left The time can be set in 0 5 minute increments from 0 to 10 minutes and in minute increments from 10 to 30 minutes After 30 minutes the display shows which indicates continuous run In this mode the centriufge will run until manually stopped To start a r
3. phenol chloroform the rotor should be cleaned immediately after use 5 4 Disinfection Should a spill of infectious materials occur within the rotor or chamber the unit should be disinfected This should be performed by qualified personnel with proper protective equipment 5 5 Replacing fuses Check the fuse when it is recommended in the Troubleshooting Guide located in this manual The fuse holder is located in the power inlet on the rear of the unit Disconnect the power cord from the power inlet Open the fuse holder drawer by inserting a small screwdriver under the tab and prying it open Remove the innermost operative fuse from its retaining tabs and replace the fuse if necessary A spare fuse is located in the outermost chamber of the fuse drawer Replace only with a fuse of exactly the same value as the original Fuse type may be found in the Technical data section of this manual 6 Troubleshooting Guide Please refer to this guide before calling for service Centrifuge will not start Possible reason Solution Possible reason Solution Lid lock will not release Possible reason Solution Possible reason Solution Possible reason Solution Possible reason Solution No power supply Check that power is being supplied to the outlet Check that the power cord is plugged into both the wall outlet and the back of the centrifuge Check that power cord is not damaged Blown fuse Check fuse and replace if
4. centrifuge has not been installed properly The centrifuge is partially dismantled Service has been attempted by unauthorized or unqualified personnel The rotor has not been installed securely on the motor shaft Rotors and accessories not belonging to the standard range are being used without permission being obtained from the manufacturer to use such rotors and or accessories 10 the centrifuge Exception Microcentrifuge tubes made of plastic normally available in the laboratory The centrifuge is located in an explosive atmosphere Materials to be centrifuged are combustible and or explosive Materials to be centrifuged are chemically reactive The rotor load is not properly balanced The rotor nut was not tightened with a wrench 1 3 Technical data Dimensions Width 9 25 inches Depth 11 5 inches Height 8 5 inches Maximum speed 13 300rpm Maximum RCF 16 300 x g Maximum volume 24 x 1 5 2 0ml Admissible sample density 1 2kg dm3 Electrical fuse rating 120V 50 60Hz 1 9A 2 5 AT 230V 50 60Hz 1 1A 1 25 AT Operating temp humidity 0 C to 40 C 80 RH 1 4 Accessories supplied with centrifuge Each unit is supplied with 1 instruction manual warranty card 1 power cord a standard rotor and a rotor removal tool 1 5 Warranty This centrifuge has been subjected to thorough testing and quality control In the unlikely event of a manufacturing fault our one year warranty from the date of delivery covers the centrif
5. exceed these values The maximum speed of the rotor has been established for liquids having a homogeneous density of 1 2g ml or less In order to centrifuge liquids with a higher density it is necessary to reduce the speed Failure to reduce the speed may result in damage to the rotor and centrifuge The revised maximum speed can be calculated with the following formula LZ Reduced speed Nre x max speed Nmax HigherDensityValue Example Where the density of the liquid is 1 7 the new maximum speed would be calculated as follows Nred X 13 300 11 174 rpm 4 Operation ATTENTION Never attempt to operate the centrifuge with rotors or adapters that show signs of corrosion or mechanical damage Never centrifuge strongly corrosive materials that may damage the rotors accessories or bowl of the unit 4 1 Attaching rotor lid After the rotor has been properly secured and loaded attach the rotor lid to the rotor Always use the rotor lid for safety and to allow the rotor to reach proper speed Make sure that the rotor lid snaps securely into place by pressing down on center catch 4 2 Closing the lid Close the centrifuge lid The Spectrafuge 24D has a lid lock that activates only when a run is started 4 3 Lid release The lid will remain locked during a centrifuge run Once the run has been completed and the rotor has come to a stop a beep will indicate the end of a run and the lid will unlock automatically
6. x a 2 1000 20000 30 Jar where r radius in centimeters n speed in RPM 10 000 6 000 na The radius from the center of the C rotation axis to the bottom or outermost portion of the test tube 3 000 should be used RCF is expressed 4 000 4 000 relative to the force of the earth s 3p00 3 gravity 20 2 000 B 3 000 18 1000 16 i 20000 500 14 fies 300 200 12 4 000 100 10 50 9 8 20 ao 7 10 6 3 5 200 To use this chart find the radius value on the radius scale Place the edge of a ruler on the value Place the right side edge of the ruler on the speed scale at the desired speed The estimated RCF can then be read from the RCF scale where the ruler edge passes through it This chart can also be used to determine the proper speed for the desired RCF value APPENDIX Symbols and Conventions The following chart is an illustrated glossary of the symbols that may be used in this manual or on the product The electrical warning indicates the presence of a potential hazard which could result in electrical shock CAUTION This symbol refers you to important operating and maintenance servicing instructions within the product Instruction Manual Failure to heed this information may present a risk of damage or injury to persons or equipment This symbol identifies a Protective Earth PE terminal which is provided for connection of the supply system s protective earth green or green yellow conductor
7. below this declaration becomes invalid Device Name Spectrafuge 24D Device Models Numbers C2400 C2400 1 00V C2400 230V Relevant EC Directives Low Voltage Directive 2006 95 EC EMC Directive 2004 108 EC RoHS 2002 95 EC WEEE 2002 96 EC Harmonized Standards EN IEC 61010 1 2001 Ed 2 IEC 61010 2 020 2006 for use with IEC 61010 1 2001 IEC 61326 1 Issued 2005 12 01 Ed 1 0 CISPR1 1 2003 IEC 61000 3 2 2000 IEC 61000 3 3 2002 IEC 61000 4 2 2001 IEC 61000 4 3 2002 IEC 61000 4 4 2004 IEC 61000 4 5 2001 IEC 61000 4 6 2003 IEC 61000 4 1 1 2004 Date July 1 2012 Peter Will gt Product Line Manager Labnet Labnet International Inc LIMITED WARRANTY Labnet International Inc warrants that this product will be free from defects in material and workmanship for a period of two 2 years from date of purchase This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in the supplied instruction manual Should this product require service contact Labnet International Inc s Service department at 732 417 0700 to receive a return authorization number and shipping instructions Products received without proper authorization will be returned All items returned for service should be sent postage prepaid in the original packaging or other suitable carton padded to avoid damage Labnet International Inc will not be responsible for damage incurred by improp
8. er packaging Labnet International Inc may elect for onsite service for larger equipment This warranty does not cover damage caused by accident neglect misuse improper service natural forces or other causes not arising from defects in original material or workmanship This warranty does not cover motor brushes fuses light bulbs batteries or damage to paint or finish Claims for transit damage should be filed with the transportation carrier ALL WARRANTIES INCLUDING THE IMPLIED WARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED IN DURATION OF 24 MONTHS FROM THE ORIGINAL DATE OF PURCHASE LABNET INTERNATIONAL INC S SOLE OBLIGATION UNDER THIS WARRANTY IS LIMITED TO THE REPAIR OR REPLACEMENT AT LABNET INTERNATIONAL INC DISCRETION OF A DEFECTIVE PRODUCT LABNET INTERNATIONAL INC IS NOT LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGE COMMERCIAL LOSS OR ANY OTHER DAMAGES RESULTING FROM THE USE OF THIS PRODUCT Some states do not allow limitation on the length of implied warranties or the exclusion or limitation of incidental or consequential damages This warranty gives you specific legal rights You may have other rights which vary from state to state No individual may accept for or on behalf of Labnet International Inc any other obligation of liability or extend the period of this warranty Mail Warranty Registration to Register online at Labnet International Inc www labnetinternational com 31 May
9. et labnetlink com Should your Spectrafuge require service please call Labnet s Technical Services Department at 732 417 0700 Our Service Department is staffed from 8 30am to 5 00pm EST Monday through Friday Our 24 hour fax number is 732 417 1750 Electronic mail may be sent to labnetinfo corning com Please have the unit s serial number located on the back panel of the instrument available when calling Should an item require return to Labnet for service a repair authorization RA number must first be received from Labnet and a decontamination form completed by the user Items sent without an RA number or decontamination form will not be accepted 8 Determination of g values The centrifuging radius of the 1 5 2 0ml rotor is 8 23cm The Spectrafuge 24D has an automatic g force conversion program so gvalues are automatically calculated and can be displayed on the control panel for this centrifuging radius If adapters or smaller tubes are used the centrifuging radius changes as does the g force The chart on the next page can be used to manually determine g values for any centrifuging radius RELATIVE CENTRIFUGAL FORCE Radius Speed in cm in RPM 50 20 000 45 Relative Centrifugal Force Relative 15 000 amaa EANGuaal 00000 Centrifugal 7 40 e relative centrifugal force Force G Force can be estimated using x g the chart on this page or by 35 applying the following formula 70 000 g 1148 xr
10. field Ave Edison NJ 08837 3 lt cut along the dotted line To validate the warranty complete and return this card within 10 days Model Serial No Date Tested Date Rec d PO Name Title Phone Institution Address City State ___ _ s Zip Postal Code Country Purchased from distributor How would you rate the quality of this product Excellent C Good O Fair I Poor What feature s on this product made you purchase it What feature s would you change to improve the performance of this product Labnet 31 Mayfield Avenue Edison NJ 08837 p 732 417 0700 f 732 417 1750 Labnet International Inc Labnet International Inc
11. necessary Defective lid lock Open manually and have unit serviced No power from PC board Call for service Lid lock is jammed Call for service Centrifuge is not receiving power See Centrifuge will not start Centrifuge cannot be started although power is on Possible reason Solution Possible reason Solution Lid not closed correctly Close lid correctly No speed or time has been selected Set speed and or time bAL Error Message Indicates imbalance Possible reason Solution Possible reason Solution Possible reason Solution Lid Error message Lid not closed Possible reason Solution Possible reason Solution Tubes not inserted symmetrically in rotor holes Load tubes symetically see Section 3 4 on Loading the rotor Sample liquid in tubes not balanced Make sure the same volume of liquid is in each tube Defective or improperly adjusted balance sensor Call service Lid not closed completely Close lid Lid lock or sensor defective Call service Other Error Messages Er on Display Solution Press time or speed knob to clear error Call service 7 Where to call Should you have any questions about the Spectrafuge or its accessories please call Labnet s Customer Service Department at 732 417 0700 Customer Service is staffed from 8 30am to 5 30pm EST Monday through Friday Our 24 hour fax number is 732 417 1750 Inquiries may also be sent via our electronic mailbox at labn
12. opectrafuge 24D Microcentrifuge ser Manua C2400 C2400 100V C2400 230V C Vea l 600 Labnet International Inc Intertek Rev 2 July 2012 Lit M00013 Safety Precautions NEVER use the centrifuge in any manner not specified 10 these instructions NEVER operate the centrifuge without a rotor properly attached to the shaft NEVER tighten rotor nut by hand only NEVER fill tubes while they are in the rotor Liquid spillage may harm unit NEVER put hands in the rotor area unless the rotor is completely stopped NEVER move the centrifuge while the rotor is spinning NEVER use solvents or flammables near this or other electrical equipment NEVER centrifuge flammable explosive or corrosive materials NEVER centrifuge hazardous materials outside of a hood or proper containment facility ALWAYS load the rotor symmetrically Each tube should be counter balanced by another tube of the same type and weight ALWAYS locate the centrifuge within easy access to an electrical outlet ALWAYS use only microcentrifuge tubes made from plastic and designed to withstand centrifugal forces of at least 16 500 xg ALWAYS use a wrench to tighten the rotor nut Table of Contents General Information Description Safety precautions Technical data Accessories supplied with unit Warranty Installation Unpacking the centrifuge Required space Installing the centrifuge Installing the Rotor Rotors and acce
13. s a buildup of heat may occur within the chamber 2 3 Installation Before operating the centrifuge check that the power source electrical outlet on the wall corresponds to that on the manufacturer s rating label then connect the power cord to the centrifuge and the power source 3 Installation of rotors and rotor maintenance 3 1 Rotors and accessories The following accessories are included or available for the Spectrafuge Angle rotor for 24 x 1 5ml tubes Included Tube measurement 1 5ml 2 0ml 10 x 40mm Max speed 13 300rpm Centrifuging radius 8 23cm Max RCF g value 16 276 x g Rotor Wrench Included Order no C0240 RT Rotor Lid Included Order no C0240 RC Rotor Securing Screw Included Order no C0240 RSS Strip Spin Adapter Order no C0240 SS Tubes accepted 0 2ml tubes or 8 x 0 2ml strips Max Speed 13 300 rpm Range Centrifuge radius 4 32cm to 5 33cm Range RCF g value 8 549 x g to 10 539 x g Adapter for 0 5ml tubes pk 6 Order no C 1205 Tube measurement 8 x 30mm Max speed 13 300rpm Centrifuging radius 7 53cm RCE g value 14 892 x g Adapter for 0 4ml tubes pk 6 Order no C 1206 Tube measurement 6 x 47mm Max speed 13 300rpm Centrifuging radius 8 23cm RCE g value 16 276 xg Adapter for 0 2ml tubes pk 6 Order no C 1222 Tube measurement 6 x 21mm Max speed 13 300rpm Centrifuging radius 7 03cm RCF g value 13 903 x g 3 2 Rotor maintenance The rotor should be cleaned
14. ssories Rotor maintenance Removing and installing the angle rotor Loading the rotor Overloading rotors Operation Attaching rotor lid Closing the lid Lid release Lid lock Speed selection RPM g force Selection of operating time momentary operation Start Stop Service and Maintenance Service Cleaning Disinfection Replacing fuses Troubleshooting Where to Call Determination of g values Appendix Symbols and Conventions Disposal of Equipment Declaration of Conformity Warranty Card 10 General Information This manual provides important safety information for the Spectrafuge 24D laboratory microcentrifuge It should be kept near the centrifuge for quick and easy reference 1 1 Description The Spectrafuge 24D is a small benchtop centrifuge designed for separation of various research samples The motor is brushless and requires no routine maintenance The Spectrafuge 24D is supplied with a 24 x 1 5 2 0ml rotor for micro samples Adapters are available for tubes smaller than 1 5ml The Spectrafuge 24D reaches speeds of up to 13 300rpm 16 300 x g 1 2 Safety precautions Note All users of the centrifuge must read the Safety Precautions section of this manual before attempting to operate the unit If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Do not operate the centrifuge if any of the following conditions exist The
15. thoroughly after each use Thorough cleaning must be performed when spinning samples containing phenol or phenol chloroform Periodically inspect the rotor for dents dings scratches discoloration and cracks If any damage to the rotor is found discontinue use of the rotor immediately and replace 3 3 Removing and installing the angle rotor The Spectrafuge 24D comes complete with a standard 24 place rotor installed To remove the rotor for cleaning remove the rotor securing screw from the motor shaft by turning the screw counterclockwise using the rotor wrench Lift the rotor directly upward in a straight vertical motion To replace rotor first make sure the motor shaft and rotor mounting hole are clean Place the rotor on the motor shaft Reinstall the rotor securing screw on the motor shaft by turning it clockwise Hold the rotor with one hand and tighten the rotor securing screw using the rotor wrench Figure 1 Loading the rotor to achieve balance 3 4 Loading the rotor Tubes to be loaded should be filled equally by eye The difference in the weight between the tubes should not exceed 0 1 gram Tubes should always be loaded so that there is equal spacing between all tubes One or two additional loaded tubes may need to be added to achieve this Refer to Figure 1 to see one typical balancing scheme 3 5 Overloading rotors The maximum load of the rotor and the maximum speed have been established by the manufacturer Do not attempt to
16. uge and the rotor This warranty becomes invalid in the case of incorrect operation use of nonstandard spare parts or accessories and unauthorized modification of the rotor or centrifuge Labnet reserves the right to make technical modifications Please see the complete limited warranty statement supplied separately 2 Installation 2 1 Unpacking the centrifuge Before unpacking the centrifuge inspect the outside of the carton for any shipping damage The centrifuge is delivered in a carton with protective foam cushioning Remove the centrifuge from the carton Retain thecarton and cushioning until it has been established that the centrifuge is working properly Inspect the centrifuge for any visible signs of shipping damage Shipping damage is the responsibility of the transportation carrier Any claims for damage must be filed within 48 hours with the carrier that delivered the centrifuge The accessories supplied with the centrifuge should be kept with the instruction manual near the centrifuge s place of installation 2 2 Required space The centrifuge should be installed on a rigid even surface such as a stable laboratory bench countertop etc To guarantee sufficient ventilation ensure that the centrifuge has at least 15cm 6 inches of free space on all sides including the rear The centrifuge should not be located in areas subject to excessive heat such as in direct sunlight or near radiators or the exhaust of a compressor a
17. un press the timer knob When the preselected time expires the centrifuge will stop automatically To stop the centrifuge prior to the expiration of set time press the timer knob The centrifuge may be operated for a short run by pressing and holding the timer knob The centrifuge will continue to run as long as the timer knob is depressed and the time in seconds will count up on the time display a i ss i TT Tite Speed REF pam al ii 0 aii ay wE l 3 H 4 Ti Se Oo Wolly SS infest Figure 2 Spectrafuge control panel layout 5 Service and Maintenance 5 1 Centrifuge service The brushless motor in the Spectrafuge 24D requires no routine maintenance Any required service should be performed by authorized qualified personnel only Repairs performed by unauthorized personnel may void the warranty 5 2 Cleaning the centrifuge Always keep the centrifuge housing rotor chamber rotor and rotor accessories clean All parts should be wiped down periodically with a soft cloth For more thorough cleaning use a neutral cleaning agent pH between 6 and 8 applied with a soft cloth Excessive amounts of liquid should be avoided Liquid should not come into contact with the motor After cleaning ensure that all parts are dried thoroughly by hand or in a warm air cabinet maximum temperature 50 C 5 3 Cleaning the rotor The rotor should be cleaned after each use When spinning samples containing phenol or
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