Home

KingFisher Flex User Manual

1. c cee ee 32 Figure 3 23 Inserting the KingFisher Flex head cceceecesseeeeceeesseeeeeees 33 Figure 3 24 KingFisher Flex head for KingFisher 24 deep well plate fitted 34 Figure 3 25 See through lid fitted 34 Figure 3 26 Refitting the transport lock of the heating block A 35 Figure 3 27 Refitting the transport lock of the heating block B 36 Figure 3 28 Fitting the transport lock of the tip comb holder A 3 Figure 3 29 Fitting the transport lock of the tip comb holder B 3 Figure 3 30 Both transport locks refitted 0 eceeceeceseesesereeerens 38 Figure 4 31 Keyboard of the KingFisher FleX s c 40 Figure 4 32 Main view on the display of the KingFisher Flex 00 00 41 Figure 4 33 ip comb package with two tip COMbS eeeeeeeen 51 Figure 4 34 Separating the two tip COMDS ceeereenrnnn 52 Figure 4 35 Storing the other tip comb on a KingFisher 96 plate for further E e EAEE E N E A EA EA ETTAN 52 Figure 4 36 PCR tip comb package ooo eceececececeeesceseseteseetetestetereeeen 53 Thermo Fisher Scientific Thermo Fisher Scientific Figures Figure 4 37 24 deep well tip comb Package s on 53 Figure 4 38 Combining the tip comb and KingFisher plate A 00 54 Figure 4 39 Combining the tip comb and KingFisher plate B 0 0 55 Figure 4 40 Loading the tip plate o oo ccc cc
2. Thermo Scientific KingFisher Flex User Manual Rev 1 2 Cat no NO7669 Copyright Copyright 2010 Thermo Fisher Scientific Corporation All rights reserved First edition published in 2008 Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Patents This product is protected by the following patents US 6447729 Method and means for magnetic particle specific binding assay and US 6448092 Separation device for microparticles involving a magnetic rod KingFisher Flex also has national and international patents pending Trademarks BindIt is a trademark of Thermo Fisher Scientific KingFisher and Microtiter are registered trademarks of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable
3. Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 57 Routine Operation Changing the heating block Head Plate KingFisher Flex head for KF 24 deep well plate KingFisher Flex head for Note The sliding door can be left open or the see through lid can be absent if desired This action does not break the run A 7 After the run remove the plate s according to the protocol request Confirm each plate removal by pressing the START key Note that the plate containing your samples is removed first 8 Press the STOP key after completing the run For startup refer to Switching on on page 40 Figure 4 45 Kingfisher Flex plates Caution Do not use other plates than the ones listed in Table 4 4 Other plates may not be compatible with the KingFisher Flex heating blocks They may also cause unexpected problems such as cross contamination due to the divergent well volume and bottom height of the plate A The KingFisher Flex is compatible with KingFisher 24 deep well plates Microtiter deep well 96 plates KingFisher 96 plates and PCR plates Figure 4 45 and Table 4 4 Specially designed magnetic rods KingFisher Flex heads and tip combs that protect the magnets during the process are available for different plate types and applications Table 4 4 Processing volumes vs plate types and magnetic heads Microtiter deep well 96 plate KingFisher 24 Microtiter deep KingFisher 96 PCR plate
4. Remo Figure 3 30 Both transport locks refitted Figure 3 30 shows both the transport lock of the heating block and the tip comb holder refitted 38 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Dos and Don ts of the KingFisher Flex Thermo Fisher Scientific Do Dont Chapter 4 Routine Operation This section on Do s and Don ts summarizes all the relevant procedures on what to do and what not to do e Incase of any emergencies occurring during operation switch off and unplug the instrument immediately Carry out corrective measures If the corrective measures taken do not help contact authorized technical service e Carry out the operational check prior to normal use e Only when the instrument is switched off can you push the heads or turn the turntable manually e When placing a microplate onto the tray always make sure that the correct plate type has been selected in BindIt Software Plate Editor gt Plate Configuration before you do anything else e Always use correct volumes on the plates because the movements of the tip comb are conducted according to the volumes e Use for self testing is excluded e Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty e Never open any other cover of the KingFisher Flex than the see through lid
5. A YY Y WAL RS 23 k Figure 3 14 Mains supply cable and serial cable connected How to fit the subassemblies This section describes the installation setups of the heating block shield of the instrument into place plate interchangeable KingFisher Flex heads and the see through lid Heating block Fastening changing the heating block is controlled by the software To fit the heating block choose the Change_heatblock protocol under the Maintenance menu by using the up and down cursor keys Then press START The heating block will thus rise Insert the heating block by first setting it and then pressing it hard into place Figure 3 15 and Figure 3 16 The heating block will snap at both ends if you have done it correctly ji SS se Figure 3 15 Inserting the heating block Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 27 Installation Installation setups Figure 3 16 Pressing the heating block into place You can add a heating step of ambient temperature 5 C to 115 C to a protocol The heating block is preheatable but no cooling can be carried out All KingFisher Flex plates can be heated There are four different kinds of interchangeable heating blocks available for KingFisher 24 deep well plates Microtiter deep well 96 plates KingFisher 96 plates and PCR plates Table 3 1 Table 3 1 Plates vs heating adapter s Plate Description Heating adapter s Description
6. Breivold E and Hornes E 1990 Purification of mRNA directly from crude plant tissues in 15 minutes using oligo dT microspheres Nucleic Acids Res 18 3669 Jakobsen K S Haugen M Szboe Larsen S Hollung K Espelund M and Hornes E 1994 Direct mRNA isolation using magnetic Oligo dT Beads A protocol for all types of cell cultures animal and plant tissues In Eds Uhl n M Hornes E and Olsvik Advances in Biomagnetic Separation Eaton Publishing Pp 61 71 Thermo Fisher Scientific Thermo Fisher Scientific References Josefsen M H Krause M Hansen F and Hoorfar J 2007 Optimization of a 12 hour TaqMan PCR based method for detection of salmonella bacteria in meat Applied and Environmental Microbiology 73 3040 3048 Kim I J et al 2003 Development and Applications of a B Catenin Oligonucleotide Microarray B Catenin Mutations Are Dominantly Found in the Proximal Colon Cancers with Microsatellite Instability Clin Cancer Res 9 2920 2925 Konthur Z and Walter G 2002 Automation of phage display for high throughput antibody development TARGETS 1 1 30 36 Lambert K N and Williamson V M 1993 DNA library construction from small amounts of RNA using paramagnetic beads and PCR Nucleic Acids Res 21 775 776 Lee Y H and Vacquier V D 1992 Reusable cDNA libraries coupled to magnetic beads Anal Biochem 206 206 207 Lund M Wedderkopp A Wa
7. Select START Run OK Details Thermo Fisher Scientific Routine Operation Factory User protocols Use the Up and Down arrow keys to select the protocol Press START to run the protocol Press OK to see the protocol details HO lt More START Run OK Back Go to the Factory protocols User protocols menu You can set your default protocol If you do not the last used protocol will be shown Setting the default protocol To set the default protocol go to the protocol list Last used Default cf ___ Last used gt Not available a DNA RNA Default Cell Protein Other Select START Run OK Details Press OK to start setting your default protocol Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 45 Routine Operation Factory User protocols More START Run OK Set Press OK to select your default protocol total DNA from tissue VW 24 DY S DNA from plant ax 96 96 DW More STOP Back OK Default AV Use the Up and Down arrow keys to select the protocol Press OK to set the default protocol OK Set START Run More Press STOP to go back Thermo Fisher Scientific 46 Thermo Scientific KingFisher Flex User Manual Routine Operation Instrument options DN AIRANA Cell Protein Other Select START Run OK Details Instrument This section describes the instrument parameters All these parameters are set in t
8. cisnsdascceterscveseixcvensediavesseientueseciex 23 Few 0 eNsute Stantu a2 a eee ees 26 How to fit the subassemblies of the instrument into place 27 Feann plock sca ae ite aan ee te aay aint ci ltd ane 27 Seld p RE eaae a E aA 29 Interchangeable KingFisher Flex heads eeeeesseeeseeeeeseeeeenees 31 See thro D 1d acs ans haat cai eee neon EE 34 Operations keie R amp cs asss tecrsacsadneatesiertouy a EO 34 How to pack the instrument for transportation ssssessesssssssssssesse 35 How to refit the transport lock of the heating block 35 Thermo Scientific KingFisher Flex User Manual 7 Contents How to refit the transport lock of the tip comb holder 36 Do s and Don ts of the KingFisher Flex 0 0 eee eeeeeeeeseeeeneeeeenees 39 Chapter 4 Routine Operation xccciesess25sceesestscicias Wasa ease eacnaee tara 39 BoM ee rr eo ere arr ere 39 DON shots eee E ere eee eee 39 Ste DT 6 0 ce ner erate ane onrre Ree rere nee ere reer nee ree 40 COMtOM Pane berinai era ee 40 Keyboard recesio sietiweve sie meazeaigceeseouiaae anata dastaeks 40 IBY E I EEE S E E A E A E E ES T 40 Dipecat aA E E R E basateaeeateauanss 4 PNAS ACU ets resets resell stata ears etch eat iatisi nati uieciedeleteewptet nace 4 Using Kine Fisher Flex PC si ware sarcetcntvcocs costeyaensesonacndeuttetermets 44 Using internal softa Geconi es meee 44 Factory 1 OSer provocals isna ime eee 44 SeLECtI OLIN POTICO ianari R 44 Serine te dela
9. 3 13 71 general 68 71 performance 71 safety 68 72 73 switching on 40 59 symbols 4 T tip comb 13 16 17 18 23 24 25 31 32 33 35 36 37 38 39 52 53 54 55 56 57 58 59 61 72 76 77 79 80 81 83 84 93 holder 23 24 25 33 35 36 37 38 57 58 76 79 80 transport 21 22 23 24 25 26 35 36 37 38 67 68 71 LT damage 22 locks 23 24 25 26 35 36 37 38 67 77 package 21 22 67 71 troubleshooting 3 79 80 81 guide 80 the instrument 80 U unpacking 21 52 54 user protocols 41 45 47 using BindIt Software 45 using internal software 45 W warnings 3 4 5 6 21 23 27 29 33 40 61 63 65 66 68 76 80 81 warranty 2 21 22 39 81 working with a magnetic rod 18 Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 97 Notes 98 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 99 Thermo Fisher Scientific Oy Ratastie 2 RO Box 100 FI 01621 Vantaa Finland www thermoscientific com N07669
10. out of position 7 Serial number already set 8 Invalid command parameter g Permanent parameters lost 10 Protocol name already used in other directory 11 Internal software error 12 The requested movement is not Continued Thermo Fisher Scientific Description The command was not recognized as a valid KingFisher Flex command The magnetic head position Is in error The turntable position is in error The magnetic tips lift position is in error The heater lift position is in error The shield plate position Is in error Attempt to set the serial number when It is already set Invalid command argument Nonvolatile parameters lost Cannot record a protocol with the same name to another directory Internal software error This error is actually never reported because the firmware halts on detection of an internal error However this error may show up in the error log Cannot perform the requested movement For example the head and Thermo Scientific KingFisher Flex User Manual 79 Troubleshooting Guide Troubleshooting guide Description the magnets cannot move down when the shield plate is in place or the turntable cannot rotate when the heater is up No plastic tips attached to the head The range of movement of the head and the magnets is limited if the tips are not attached to the head This is to prevent bringing the magnets In direct contact with the magnetic particle solution The magnets are missin
11. transport package Operating conditions indoor use Transportation conditions storage conditions Mains power supply Power consumption Heat dissipation Computer interface Internal software Normal use ca 680 mm W x 600 mm D x 380 mm H 26 8 W x 23 6 D x 15 H 800 mm W x 1200 mm D x 710 mm H 31 5 W x 47 2 D x 28 H ca 28 kg 62 Ibs ca 44 kg 97 lbs 5 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only 40 C to 60 C packed in transport packaging 25 C to 50 C packed in transport packaging 100 240 Vac 50 60 Hz nominal Automatic voltage detection 150 VA max 55 VA standby 341 BTU max USB or serial RS 232C port Baud rate 9600 Character format 1 start bit 8 data bits 1 stop bit and no parity Flow control XON XOFF Space for max 512 internal protocols 10 runs day 250 days year with 40 min protocols having a medium speed setting RT Performance The performance specifications are presented in Table 6 7 specifications Table 6 7 Performance specifications Performance specifications Processing volume Continued 24 200 ul 5 mL 96 20 1000 ul Thermo Scientific KingFisher Flex User Manual 71 Technical Specifications Safety specifications In conformity with the requirements Safety specifications Cont Performance speci
12. 8 8 Error messages reported 79 Table 8 9 Actions taken against error messages and warningS 80 Table 9 10 Codes for products 0 e ioe eceeeccceecesessesessesesestesetereteseseetenes 83 Table 9 11 Codes for accessories and consumables 83 Table 9 12 Codes for spare parts wuts carste ti see ese acre eccaiacelte eaeehia nae gions 84 Thermo Fisher Scientific Chapter 1 Introduction to the KingFisher Flex Intended US The KingFisher Flex magnetic particle processor Figure 1 1 is intended for professional research use by trained personnel The instrument is intended for automated transfer and processing of magnetic particles in a microplate scale Use for self testing is excluded It is recommended that Good Laboratory Practices GLP are followed to guarantee reliable analyses Refer to Chapter 6 Technical Specifications Principle of The KingFisher Flex magnetic particle processor Figure 1 1 is operati on designed for automated transfer and processing of magnetic particles in microplate format The patented technology of the KingFisher Flex system is based on the use of magnetic rods covered with a disposable specially designed tip comb and plates The instrument functions without any dispensing or aspiration parts or devices Samples and reagents including magnetic particles are dispensed into the plates according to the corresponding instructions The protocol that is selected by th
13. 96 KF head and heating block KingFisher Flex 96 deep well head and deep well and KF heating blocks KingFisher Flex 24 deep well head and heating block KingFisher Flex 96 PCR head KingFisher Flex KF head KingFisher Flex 96 deep well head KingFisher Flex 24 deep well head KingFisher Flex 96 PCR heating block KingFisher Flex 96 KF heating block KingFisher Flex 96 deep well heating block KingFisher Flex 24 deep well heating block KingFisher Flex 96 tip comb for PCR magnets Thermo Scientific KingFisher Flex User Manual Quantity 1 1 1 1 1 80 pcs 83 Ordering Information List of spare parts List of spare parts 84 Thermo Scientific KingFisher Flex User Manual Cont Code 97002524 97002534 97002610 97002540 95040450 95040460 95040470 95040480 Item KingFisher Flex 96 tip comb for KF 96 magnets KingFisher Flex 96 tip comb for DW magnets KingFisher Flex 24 deep well tip comb and plate KingFisher 96 KF plate 200 ul Microtiter deep well 96 plate V bottom Microtiter deep well 96 plate V bottom sterile KingFisher Flex 24 deep well plate KingFisher Flex 24 deep well plate sterile Table 9 12 Codes for spare parts Code 2305290 N04001 Item Serial cable F9 F25 for RS 232C port USB A B device cable 1 8 m Longer USB cables available from PC stores Quantity 100 pcs 100 pcs 50 pcs of each 48 pcs 50 pcs 50 pcs 50 pcs 50 pcs Quantity 1 1 Thermo Fisher Scientific K
14. KingFisher Flex heads Do not hold the KingFisher Flex heads close to a PC display since this may cause damage to the display Do not use metal tools when handling KingFisher Flex heads Be careful not to break the magnets while cleaning A Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field A Thermo Scientific KingFisher Flex User Manual 65 Maintenance Disposal of materials How to clean the shield plate Disposal of materials Decontamination procedure A 66 Thermo Scientific KingFisher Flex User Manual If required wipe the shield plate using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol The shield plate is autoclavable at 1 bar pressure and 121 C for 20 minutes Follow laboratory and country specific procedures for the disposal of biohazardous or radioactive waste Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used plates disposable gloves syringes disposable tips and so on as biohazardous waste A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed A deco
15. Overall O oO Additional instrument and or software features desired Did you encounter any problems As expected Below expectations Comments O O OOoOddadda OOOdddQ Where did you first learn about the product Would you like to receive information about other Thermo Scientific products Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 91 Thermo Scientific KingFisher Flex Feedback Form 92 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Glossary heating block There are four different kinds of interchangeable heating blocks available that is for KingFisher 24 deep well plates Microtiter deep well 96 plates KingFisher 96 plates and PCR plates Figure 4 48 KingFisher Flex head There are four kinds of interchangeable KingFisher Flex heads available for KingFisher 24 deep well plates Microtiter deep well 96 plates KingFisher 96 plates and PCR plates The KingFisher Flex heads all have corresponding disposable plastic tip combs magnetic bead magnetic particle The magnetic particles attach to the magnetic rods Figure 2 7 and Figure 2 8 that are protected by a disposable tip comb The magnetic particles enable the purification of a variety of target molecules magnetic rod The rods which are magnetic and collect magnetic particles Figure 4 43 The rods do not collect the particles on their own the magnetic rods must always be protected
16. WARNING DISCONNECT SUPPLY BEFORE SERVICING AVERTISSEMENT COUPER L ALIMENTATION AVANT L ENTRETIEN ET LE DEPANNAGE Thermo Fisher Scientific Warning and other markings used in the documentation Thermo Fisher Scientific DEBE About This User Manual safety symbols and markings The following symbols and markings appear in this user manual Warning Risk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A Thermo Scientific KingFisher Flex User Manual 5 About This User Manual Instrument safety and guidelines for use Instrument safety and guidelines for use 6 Thermo Scientific KingFisher Flex User Manual Always follow basic safety precautions when using the KingFisher Flex to reduce the risk of injury biohazardous contamination fire or electric shock Read this user manual in its entirety prior to operating the instrument Failure to read understand and follow the instructions in the manual may result in damage to the instrument injury to laboratory and operating personnel or poor instrument performance Observe all Warning Caution and Note statements as well as safety symbols and markings on the instrument and i
17. back How to handle To handle 96 deep well tip combs correctly follow these steps tip combs 1 Take a 96 deep well tip comb package containing two tip combs Figure 4 33 Sea A Figure 4 33 Tip comb package with two tip combs 2 Unpack the package by separating the two tip combs Figure 4 34 and placing one of the tip combs onto the KingFisher 96 plate tip plate Figure 4 38 and Figure 4 39 Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 51 Routine Operation How to handle tip combs Figure 4 34 Separating the two tip combs 3 Place the other unused tip comb onto another plate for storage to avoid bending of the tip combs Figure 4 35 to ensure proper instrument operation Figure 4 35 Storing the other tip comb on a KingFisher 96 plate for further use To handle PCR tip combs correctly follow these steps 1 Take a PCR tip comb package containing eight tip combs within each other on a screen plate Figure 4 36 52 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation How to handle tip combs Figure 4 36 PCR tip comb package 2 Unpack the package take out one PCR tip comb and place it ona KingFisher plate If the tip combs do not load properly refer to Q 6 on page 77 To handle 24 deep well tip combs correctly follow these steps 1 Take a 24 deep well tip comb package where the unattach
18. by a tip comb plate The disposable plates 1 8 where all the reagents and samples are located and where the processing takes place Four different types of 24 and 96 well plates Figure 4 45 that can be used are as follows e KingFisher 24 deep well plate 200 pl 5 mL e Microtiter deep well 96 plate 50 1000 pl e KingFisher 96 plate 20 200 ul e PCR plate 20 100 ul skirted recommended filling volume shield plate When the turntable moves the shield plate moves over the plate underneath Figure 4 44 forming a protective cover Thermo Fisher Scientific tip comb Protects the magnetic rods 24 or 96 ina frame A disposable tip comb always has to be placed onto the microplate prior to processing There are four different kinds of disposable tip combs available e for KingFisher 24 deep well plates e for Microtiter deep well 96 plates e for KingFisher 96 plates e for PCR plates turntable A turntable with eight plate stations that can be detached for cleaning purposes Figure 2 2 Thermo Scientific KingFisher Flex User Manual 93 Glossary 94 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Index A accessories 21 22 83 application 2 5 91 BindIt Software 3 13 14 21 39 43 45 55 81 83 buzzer 44 50 51 C Cell 43 47 Certificate of Decontamination 67 68 81 82 89 changing the heating block 59 clean 22 63 6
19. carefully with the arrows on the transport package pointing upwards Remove the instrument from the package and place it on a level surface Refer to the enclosed unpacking instructions The following notes and instructions are sent with the instrument and are immediately available when you open the package e Unpacking instructions e KingFisher Flex Feedback Form e Warranty Certificate card e Packing instructions Packing list e Thermo Scientific KingFisher Flex User Manual and Quick Reference Guide e Transportation discrepancy report e BindIt Software package Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so might cause misalignment and will void the instrument warranty A Warning The KingFisher Flex weighs approximately 28 kg 62 lbs without the transport package and should be lifted with care It is recommended that two persons lift the instrument together taking the proper precautions to avoid injury A To lift the instrument put your fingers under the bottom on either sides and lift it with your back straight Thermo Scientific KingFisher Flex User Manual 21 Installation Requirements Checking delivery Requirements 22 Thermo Scientific KingFisher Flex User Manual Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternati
20. deep well plate well 96 plate plate skirted 200 5000 ul 50 1000 ul 50 150 ul KingFisher Flex head for KF 96 plate 20 200 ul KingFisher Flex head for PCR plate 20 200 ul 20 100 ul Changing the heating block 58 Thermo Scientific KingFisher Flex User Manual Changing the heating block is controlled by the software To change the heating block choose the Change_heatblock protocol by using the up and down cursor keys Then press START The protocol requests you to remove the magnets and in the end to insert them The heating block will thus rise Remove the heating block and then replace it Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Changing the heating block Figure 4 46 and Figure 4 47 Insert the heating block by first setting it and then pressing it hard into place The heating block will snap at both ends if you have done it correctly Press the START key when the task has been completed Figure 4 47 Pressing the heating block into place You can add a heating step of ambient temperature 5 C to 96 C toa protocol The heating block is preheatable but no cooling can be carried out All KingFisher Flex plates can be heated Thermo Scientific KingFisher Flex User Manual 59 Routine Operation shutdown There are four different kinds of interchangeable heating blocks available for KingFisher 24 deep well plates Microtiter deep
21. or the sliding door Figure 2 3 while the instrument is plugged into a power soutce e Do not disconnect the RS 232C or USB cable before the PC and the instrument are switched off e Do not use the instrument if it appears that it does not function properly e Do not under any circumstances use formaldehyde e Do not spill any alkalines onto any instrument surfaces to avoid damage of the instrument If needed use suitable protection covering Thermo Scientific KingFisher Flex User Manual 39 Routine Operation Switching on Switching on Control panel Keyboard Arrow keys and OK button Keys AY lt gt 40 Thermo Scientific KingFisher Flex User Manual Before you switch on the KingFisher Flex ensure that the voltage on the type label at the bottom left of the back side panel Figure 2 4 corresponds to the local voltage Warning Never operate your instrument from a power outlet that has no ground connection A This section describes the KingFisher Flex control panel and internal software The keyboard is shown in Figure 4 31 START PAUSE and STOP function buttons KINGFISHER FLEX Q TURNTABLE ROTATING CLOCKWISE and COUNTERCLOCKWISE buttons Figure 4 31 Keyboard of the KingFisher Flex The relevant keys and control buttons are described in detail below The arrow keys are used to select the next protocol and to navigate in the display To accept the selection To ini
22. storage site on the heating block base The two screws on the heating block base guide the heating block into the correct position future transportation of the instrument A Note Keep the transport locks Figure 3 11 and Figure 3 13 for Both the transport locks have been successfully removed When relocating the instrument refer to How to refit the transport lock of the heating block on page 35 and How to refit the transport lock of the tip comb holder on page 36 How to ensure 1 Connect the mains supply cable to the mains power connector startup Figure 3 14 at the bottom left of the back side panel If you need to use any other type of mains supply cable than supplied use only cables certified by the local authorities Before you plug in the power cable ensure that the voltage on the type label at the bottom left of the back side panel Figure 2 4 corresponds to the local voltage 26 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Installation Installation setups 2 Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded Warning Ensure that the mains switch Figure 2 4 on the bottom left of the back side panel is in the OFF position Never operate your instrument from a power outlet that has no ground connection A gt Caution Always connect or disconnect the serial cable when the power is off A f n lt
23. the lock and press tightly on both sides of the lock according to Figure 3 10 so that the lock ends are released Keep hold of the tip comb holder and lock simultaneously and start gradually lifting the holder Figure 3 10 Removing the transport lock of the tip comb holder 2 Lift the tip comb holder higher and continue pressing the transport lock of the tip comb holder Remove the transport lock from its Thermo Fisher Scientific Thermo Fisher Scientific Installation Installation setups location by ejecting it from the two eccentric pins Figure 3 11 When relocating the instrument refer to How to refit the transport lock of the tip comb holder on page 36 Yy TAZ kad AAA A MS A Fal j Two eccentric pins P a at o O o a5 CAUTION Remoy e Trans Before t older transport lock removed if Figure 3 11 ip comb 3 The transport lock of the heating block is removed according to Figure 3 12 Unscrew the transport lock screw counterclockwise Figure 3 12 Lift the screw with the label attached Figure 3 13 _ Figure 3 12 Removing the transport lock of the heating block A Thermo Scientific KingFisher Flex User Manual 25 Installation Installation setups 4 Remove the transport lock screw from the label Figure 3 13 Figure 3 13 Removing the transport lock of the heating block B 5 Then fasten the screw clockwise to its
24. to the outside of the package containing the instrument Refer to Appendix A Certificate of Decontamination and Decontamination procedure on page 66 Failure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Refer to Appendix A Certificate of Decontamination 82 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific KingFisher Flex List of accessories and consumables Thermo Fisher Scientific Chapter 9 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Ordering information codes are presented in Table 9 10 through Table 9 12 Table 9 10 Codes for products Code 5400610 5400620 5400630 5400640 Instrument System KingFisher Flex 96 PCR head KingFisher Flex 96 KF head KingFisher Flex 96 deep well head KingFisher Flex 24 deep well plate Table 9 11 Codes for accessories and consumables Code NO7669 N07974 5189010 24074411 24074421 24074431 24074441 24074410 24074420 24074430 24074440 24075410 24075420 24075430 24075440 97002514 Continued Item KingFisher Flex User Manual Bindlt Software User Manual Bindlt Software package KingFisher Flex 96 PCR head and heating block KingFisher Flex
25. 5 67 81 magnetic rods 65 shield plate 66 turntable 63 cleaning the magnetic rods 65 cleaning the shield plate 66 cleaning the turntable 63 computer interface 44 49 71 concentration 14 18 19 control panel 40 D decontaminating the instrument 61 66 68 81 82 89 decontamination 61 66 67 68 81 82 89 decontamination procedure 61 66 67 68 81 82 89 default factory protocol name 43 45 46 47 default user protocol name 43 45 46 47 deep well plate 28 29 31 32 34 54 59 61 72 75 76 83 93 device information 43 48 display 13 16 23 32 34 40 41 63 65 72 75 85 87 disposal of instrument 68 disposal of materials 8 66 DNA RNA 43 47 E ensuring startup 26 Thermo Fisher Scientific error message 79 80 F factory protocols 41 45 47 feedback form 21 91 H handling tip combs 52 heating block 23 25 26 27 28 29 35 36 38 59 60 61 72 76 79 80 83 93 icon 42 43 incubation 18 installation 3 21 22 23 27 29 67 73 91 installing the KingFisher Flex 21 23 73 instrument layout 15 instrument options 48 intended use 3 13 inverse magnetic particle processing 17 K keyboard 13 40 72 keys 27 33 40 41 42 45 46 47 48 49 50 51 52 55 59 25 70 keywords for web pages 85 L language 44 50 literature 86 loading position 56 magnetic particles 13 14 17 18 77 85 93 collection
26. A Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation How to start aj Plate station 1 ERAI io pF m y Ma Loading postion plate station 4 S Figure 4 39 Combining the tip comb and KingFisher plate B The loading position that is plate station 4 is labeled The eight plate stations and the Al positions of the eight plate stations are clearly marked on the turntable When the instrument is in its basic position plate station 1 is under the KingFisher Flex head After the protocol has been run note that the turntable may stop in a different position than the basic position Figure 4 40 Loading the tip plate Thermo Scientific KingFisher Flex User Manual 55 Routine Operation How to start 56 Thermo Scientific KingFisher Flex User Manual k Figure 4 41 Loading the plates Magnetic rods Tip plate o Figure 4 42 Plates loaded 4 The tip comb is automatically locked onto the tip comb holder from the tip plate Figure 4 43 Thermo Fisher Scientific Routine Operation How to start Tip comb in the tip comb holder Figure 4 43 ip comb in the tip comb holder 5 When the turntable moves the shield plate moves over the plate underneath Figure 4 44 forming a protective cover Figure 4 44 Shield plate in function 6 Close the sliding door The see through lid if in place protects the instrument against environmental contamination
27. GTE em T Sa aT r Deutch PIN te ea Francais Portugal 5elect OK Accept A Use the Up and Down arrow keys to select the language in use The available languages are English Deutsch Francais Espanol and Portuguese The default language is English Press OK Buzzer You can choose whether or not the instrument produces a sound for different functions Note that all functions make a certain sound Go to the Maintenance menu Select the Buzzer on off row 711 14 Not active English OK Buzzer onloff Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 49 Routine Operation Instrument options qp Select OK Accept Use the Left and Right arrow keys to select whether the buzzer audible q gt sound is On or Off Press OK Maintenance protocol You can set which protocol is automatically selected as the maintenance protocol when the KingFisher Flex is powered on al A Go to the Maintenance menu Select the Maintenance protocol row Not active English sie lt a hino OK Maintenance protocol Press OK All available maintenance protocols will be displayed 50 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Routine Operation How to handle tip combs Select START Run OK Back AV Use the Up and Down arrow keys to select the maintenance protocol Press START to run the desired protocol Press OK to go
28. Heating block for KingFisher 24 deep well plate KingFisher 24 deep well plate 200 pl 5 mL Heating block for Microtiter 96 deep well plate Microtiter deep well 96 plate 50 1000 ul KingFisher 96 plate 20 200 ul Heating block for KingFisher 96 plate gt PCR plate 20 100 ul skirted Heating block for PCR plate gt for example AB gene SuperPlate AB 2800 recommended filling volume 28 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Installation Installation setups Warning The heating block surface can be hot whereby there can be risk of burns A Caution The heating block is specifically designed for the plates listed below to ensure even heating during the sample process Figure 3 17 Using other plates than those recommended may damage the instrument and diminish the application performance A gt Figure 3 17 Sectional view of the Microtiter deep well 96 plate a deep well 96 plates of other manufacturers b c d and the KingFisher Flex 96 deep well heating block You can only use the following 24 and 96 well plates for heating e KingFisher 24 deep well plate 200 pl 5 mL e Microtiter deep well 96 plate 50 1000 ul e KingFisher 96 plate 20 200 pl e PCR plate 20 100 ul skirted recommended filling volume Shield plate To install the shield plate follow these steps 1 Ensure that the power is
29. Warning Timer expired protocol Troubleshooting Guide service request protocol Press the STOP button twice Check if the tips are present If it looks all right turn ON and OFF and run the check protocol according to the KingFisher Flex head and plastics you are using see Operational check on page 34 Check that the magnets are fully inserted Contact authorized technical service Delete some unnecessary protocols from the internal memory with Bindlt software and try again Repeat this procedure until the protocol fits It is recommended that you clean up the program memory from unnecessary protocols about once a month to prevent this error from occurring Description Contact authorized technical service If the KingFisher Flex requires service contact your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Do not under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Thermo Fisher Scientific representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department The Warranty Claim Technical Sheet contains a more detailed description of the fault symptom or condition Giv
30. ack ViQW ccccceccececcesessesesecstesseneetestesteneenens 16 Figure 2 5 KingFisher Flex side VIQW ccecce 16 Figure 2 6 KingFisher Flex magnetic particle processor 17 Figure 2 7 Inverse magnetic particle processing 17 Figure 2 8 A concentration step during magnetic particle processing 19 Figure 3 9 Kingfisher Flex transport locks fitted 2 0 eee eee 24 Figure 3 10 Removing the transport lock of the tip comb holder 24 Figure 3 11 ip comb holder transport lock removed cccccceeeeees 25 Figure 3 12 Removing the transport lock of the heating block A 29 Figure 3 13 Removing the transport lock of the heating block B 26 Figure 3 14 Mains supply cable and serial cable connected 00 27 Figure 3 15 Inserting the heating DIOCK uere 21 Figure 3 16 Pressing the heating block into Place o n 28 Figure 3 17 Sectional view of the Microtiter deep well 96 plate a deep well 96 plates of other manufacturers b c d and the KingFisher Flex 96 deep well heating block isecen 29 Figure 3 18 Shield plate prior to fitting oo eee cece eteeeeeee een 30 Figure 3 19 Inserting the shield plate ec cececeecscestereeeeseeseeeenrens 30 Figure 3 20 Fastening loosening the shield plate eee eee 31 Figure 3 21 Shield plate fitted ooo ccc cescecesesessesesteeesteereeeen 31 Figure 3 22 Kingfisher Flex heads in their storage DOXES
31. ag Ensure that the cloth does not make contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hours Remove the instrument from the bag Clean the instrument using a mild detergent Remove any stains using 70 ethanol 11 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package see Appendix A Certificate of Decontamination To pack for service follow the guidelines presented below Caution It is important that the instrument is thoroughly decontaminated before it is removed from the laboratory or any servicing is performed on it A When you ship the instrument for service remember to Inform about the use of hazardous materials Decontaminate the instrument beforehand Install the transport locks Place the KingFisher Flex head into its transportation box Pack the instrument according to the enclosed packing instructions Thermo Scientific KingFisher Flex User Manual 67 Maintenance Service contracts e Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges e Enclose a dated and signed Certificate of Decontamination see Appendix A Certificate of Decontamination both inside and attached to the outside of the package in which you retu
32. and the tip comb is placed onto a KingFisher plate from which it is automatically loaded The plates are placed onto the turntable into the corresponding plate stations according to the protocol instructions Thermo Fisher Scientific Principle of magnetic particle processing Thermo Fisher Scientific Functional Description Principle of magnetic particle processing During the operation the sliding door can be closed or open Figure 2 3 or the whole see through lid can be absent The closed door protects the processing against environmental contamination The operating principle employed is MPP inverse magnetic particle processing technology Figure 2 7 Rather than moving the liquids the magnetic particles are moved from plate to plate containing specific reagents in contrast to the external magnet method Magnetic particles are transferred with the aid of magnetic rods covered with a disposable specially designed plastic tip comb Figure 2 6 Kingfisher Flex magnetic particle processor Magnet Magnet S ow up and down collecting movement Fast up and down releasing movement Transfer Reagent 1 amp i i Reagent 2 amp Magnetic particles Magnetic particles e Well1 m Well 2 ______ Figure 2 7 Inverse magnetic particle processing Thermo Scientific KingFisher Flex User Manual 17 Functional Description Working with a magnetic rod Working witha magnetic rod Collect
33. aning purposes Figure 5 49 through Figure 5 51 Unscrew the two finger screws Figure 5 49 Note that the screws still remain attached to the turntable when the screws are unfastened Figure 5 49 Untastening the two finger screws Lift the turntable off Figure 5 50 Figure 5 50 Removing the turntable 64 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Thermo Fisher Scientific How to clean the magnetic rods A A Maintenance Regular and preventive maintenance If you have spilt infectious agents on the turntable clean it with a cloth dampened with water or a mild detergent Clean the black spill shield Figure 5 51 and the turntable using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol Alignment stud Figure 5 51 Black spill shield exposed for cleaning When you replace the turntable insert the turntable so that you place it first onto the aligning stud Then fasten the two finger screws You can gently rotate the turntable while the instrument is switched off If required wipe the magnetic rods using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol Caution The KingFisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the
34. ation Emergency situations 2 Wipe the turntable surface and the adjacent instrument surface with a soft cloth or tissue paper moistened with distilled water a mild detergent SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agents on the turntable disinfect with 70 ethanol or another disinfectant see Decontamination procedure on page 66 In case there is any abnormal situation during operation such as fluids spilling inside the instrument follow the steps below Switch OFF the instrument Figure 2 4 Unplug the instrument immediately from the power supply Figure 2 4 Carry out appropriate corrective measures However do not disassemble the instrument If the corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Scientific KingFisher Flex User Manual 61 Routine Operation Emergency situations 62 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Regular and preventive maintenance A How to clean the Thermo Fisher Scientific turntable Chapter 5 Maintenance For reliable daily operation keep the instrument free of dust and liquid spills Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance A soft clo
35. e It is very important to keep the KingFisher Flex heads away from each other and other magnets at all times Clashing of the magnets together may cause serious damage to the magnets A Thermo Scientific KingFisher Flex User Manual 31 Installation Installation setups Table 3 2 Magnetic head vs tip comb Magnetic head Description Tip comb Description KingFisher Flex head for KingFisher 24 Tip comb for KingFisher 24 deep deep well plate well plate KingFisher Flex head for Microtiter ih Tip comb for Microtiter deep ANI deep well 96 plate th RNR h Ny well 96 plate KingFisher Flex head for KingFisher 96 han Tip comb for KingFisher 96 plate plate Pin hinbibihig KingFisher Flex head for PCR plate Tip comb for PCR plate When not in use keep the KingFisher Flex heads always in their respective plastic storage boxes Figure 3 22 Figure 3 22 KingFisher Flex heads in their storage boxes Caution The KingFisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the KingFisher Flex heads Do not hold the KingFisher Flex heads close to a PC display since this may cause damage to the display Do not use metal tools when handling KingFisher Flex heads A 32 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Installation Installation setup
36. e all the necessary information to the distributor who will fill out and forward the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department Check Packing for service on page 67 You will find instructions on how to proceed before shipping the instrument for service to Thermo Fisher Scientific Oy Check that any necessary decontamination procedure has been carried out before packing Refer to Decontamination procedure on page 66 and Certificate of Decontamination on page 82 Ensure that the Certificate of Decontamination see Appendix A Certificate of Decontamination as well as the return authorization number RGA are sent with the instrument The Thermo Fisher Scientific technical service department will keep you up to date with the progress of service and provide you with any further details you might need for example on maintenance serviceability troubleshooting and replacement Thermo Scientific KingFisher Flex User Manual 81 Troubleshooting Guide Certificate of Decontamination Certificate of The decontamination procedure is required before shipping the Th i h fi k fi f instrument to hermo Fisher Scientific Oy for example for repair If Decontamination for any reason the instrument is shipped back to Thermo Fisher y pp Scientific Oy it must be accompanied by a dated and signed Certificate of Decontamination which must be both enclosed and attached
37. e user via the keyboard and display have already been preloaded into the onboard software BindIt Software can be used to create and run protocols Figure 1 1 Kingfisher Flex magnetic particle processor Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 13 Introduction to the KingFisher Flex Advantages of using KingFisher Flex Advantages of using KingFisher Flex 14 Thermo Scientific KingFisher Flex User Manual The KingFisher Flex magnetic particle processor has several operational advantages Enables automation of complicated manual steps Enables simultaneous processing and purification Binding and elution are enhanced due to the heating option Enables concentration of the sample during processing Facilitates a good collection of bead bound sample due to the efficiency of the magnet Enables a quicker reaction and a more efficient wash due to the technology of moving magnetic particles instead of liquids No carryover No cross contamination Facilitates the whole processing with the aid of an internal program BindIt Software Thermo Fisher Scientific Chapter 2 Functional Description Instrument This section shows the front back and side views of the KingFisher Flex ay 0 ut instrument Front view The front views of the KingFisher Flex instrument are shown in Figure 2 2 and Figure 2 3 Interchangeable KingFisher Flex head Display Loading station See through lid Sliding door
38. ececesesecstesssestestestensensetens 55 Figure 4 41 Loading the plates 0 0 cccececcscesessesessestesseeeteseestensetens 56 Figure 4 42 Plates loaded ooo ccececccccccescescsccscessesssseseesseseaseneatesteatensenens 56 Figure 4 43 Tip comb in the tip comb holder 5 Figure 4 44 Shield plate in function ooo eee ee eee eecesccesesteeeeteeeneeeen 57 Figure 4 45 KingFisher Flex plates arreen 58 Figure 4 46 Inserting the heating blok 59 Figure 4 47 Pressing the heating block Into place ec ceecceeeeeeee 59 Figure 4 48 Four different heating blocks available 0 0 0 0 cece 60 Figure 5 49 Untastening the two finger SCrE WS cccccccceeeseeseeeeeeeee 64 Figure 5 50 Removing the turntable oo cece escescestereeseseestenetens 64 Figure 5 51 Black spill shield exposed for cleaning 65 Thermo Scientific KingFisher Flex User Manual 11 Tables 12 Thermo Scientific KingFisher Flex User Manual Tables Table 3 1 Plates vs heating adapter S 0 0 cece eee ee eeeeeseeeeeeees 28 Table 3 2 Magnetic head VS tip COMD eccccecceeeccssesseseeeeerestestenenneen 32 Table 4 3 Icons in the Main VIeW epee One 43 Table 4 4 Processing volumes vs plate types and magnetic heads 58 Table 4 5 Heating blocks vs plate types cccececeseeseccssessessetetestenenneen 60 Table 6 6 General See CAO Sees os ance Redacted ences eit 71 Table 6 7 Performance specifications 71 Table
39. echnical service Contact authorized technical service Either record to the directory where the protocol is or delete the original protocol before recording this new one Contact authorized technical service Cont Code Error message allowed 13 The plastic tip comb is not attached to the holder 14 The magnetic head is not inserted to holder 15 The command is not recordable 16 Not enough memory available for recording operation Code Warning 100 Timer expired Troubleshooting a in Table 8 9 guide Code Error message 0 No error _ 1 The command was not recognized as a valid command 2 The tip comb holder lifting mechanism is out of position 3 The turntable rotating mechanism is out of position 4 The magnetic head holder lifting mechanism is out of position 5 The heating block lifting mechanism is out of position 6 The shield plate turning mechanism is out of position 7 Serial number already set 8 Invalid command parameter g Permanent parameters lost 10 Protocol name already used in other directory 11 Internal software error Continued 80 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific 15 16 Code 100 Service request Thermo Fisher Scientific The requested movement is not allowed The plastic tip comb is not attached to the holder The magnetic head is not inserted to holder The command is not recordable Not enough memory available for recording operation
40. ed A Q9 How long does it take the heater to warm up from RT to 80 C A9 The heating block warms up about 10 degrees per minute so it will take about 6 minutes Thermo Fisher Scientific Thermo Fisher Scientific Frequently Asked Questions Q amp AS Q10 What should I do if the magnets set are dirty A10 Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution soap solution or alcohol Q11 What should I do if I forget to insert the tip combs into the tip comb holder A11 The protocol will not start without the tip combs inserted into the tip comb holder Q12 What if the magnetic particles remained in the sample well A12 If the starting material is too viscose the magnetic rods will not be able to collect the particles Dilute the sample and check that the sample is properly homogenized lysed Adding low amounts of detergent will improve the collection of magnetic particles as well Quickly centrifuge the plate to sediment the particles to the bottom of the plate Q13 What if the magnetic particles are attached to the tip combs after the run A13 This happens sometimes but it will not affect the yield because the sample has been released from the particles Q14 Are the volumes of reagents in each well critical A14 It is strongly recommended that you keep the specified volumes within the defined limits to avoid spillover in the performance of the chemical reaction
41. ed tip comb is packed onto a KingFisher 24 deep well plate Figure 4 37 Figure 4 37 24 deep well tip comb package 2 Unpack the package 3 Before you take the tip comb into use press the tip comb evenly against the enclosed 24 deep well plate with the aid of a magnetic head or another object Thermo Scientific KingFisher Flex User Manual 53 Routine Operation How to start 54 Thermo Scientific KingFisher Flex User Manual How to Start To start the instrument follow these steps Select a protocol by using the cursor keys and press START OR use BindIt Software to run the desired protocol via the PC Open the sliding door if the see through lid is in place Figure 2 3 Load the plates in the order that the protocol requests Figure 4 40 through Figure 4 42 Place the A1 well of the plate so that it is in the upper right corner The first Al row is consequently always in the inner circle Once you have loaded the requested plates into the plate stations press START The tip comb always has to be placed manually onto a KingFisher plate Figure 4 38 through Figure 4 40 The instrument also functions with either one plate or up to eight plates depending on the amount of steps Only one tip comb is placed onto a KingFisher plate tip plate per run Figure 4 39 Confirm the plate loading by pressing START e T aii B a P en yan ye TA t Figure 4 38 Combining the tip comb and KingFisher plate
42. ent temperature range is between 5 C 41 F and 40 C 104 F e Make sure relative humidity is between 10 and 80 non condensing Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation The KingFisher Flex does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Caution Do not operate the instrument in an environment where potentially damaging liquids or gases are present A Thermo Fisher Scientific Precautions and limitations L A Installation setups Releasing the transport locks Thermo Fisher Scientific Installation Precautions and limitations Place the instrument on a normal laboratory bench The net weight of the entire equipment is approx 28 kg 62 lbs The instrument operates at voltages of 100 240 Vac and the frequency range of 50 60 Hz Always ensure that the local supply voltage in the laboratory conforms to that specified on the type label on the back of the instrument Figure 2 4 e Do not smoke eat or drink while using the KingFisher Flex e Wash your hands thoroughly after handling test fluids e Observe normal laboratory procedures for handling potentially dangerous samples e Wear proper protection clothing such as disposable gloves and laboratory coats according to good laboratory practice e Ensure that the working area is well vent
43. erichia coli O157 H7 through the formation of sandwiched complexes with immunomagnetic and fluorescent beads Journal of Rapid Methods and Automation in Microbiology 13 269 282 Thermo Scientific KingFisher Flex User Manual 87 References Literature Valenzuela D M et al 2003 High throughput engineering of the mouse genome coupled with high resolution expression analysis Nature Biotechnology 21 6 652 659 Wan L Battle D J Yong J Gubitz A K Kolb S J Wang J and Dreyfuss G 2005 The survival of motor neurons protein determines the capacity for snRNP assembly Biochemical deficiency in spinal muscular atrophy Molecular and Cellular Biology 25 5543 5551 Ward L I Fenn M G E and Henry C M 2004 A rapid method for direct detection of Polymyxa DNA in soil Plant Pathology 53 485 490 Ye J D Tereshko V Frederiksen J K Koide A Fellouse F A Sidhu S S Koide S Kossiakoff A A and Piccirilli J A 2008 Synthetic antibodies for specific recognition and crystallization of structured RNA PNAS 105 82 87 88 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Appendix A Certificate of Decontamination Name Address Tel Fax Name Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items
44. ether with the tip comb are designed to have minimized liquid carrying properties To keep the magnetic particle suspension evenly mixed in long running reactions the tip comb can be moved up and down in the solution The volume of the first plate can be larger than the volume of the next plate and this is used for concentration purposes see Figure 2 8 below Thermo Fisher Scientific Thermo Fisher Scientific Functional Description Working with a magnetic rod Magnet Magnet Slow up and down collecting movement Fast up and down releasing movement Tip Transfer Tip Collection of magnetic particles Release of magnetic particles from a large volume well into a low volume well m Well1 m Well2 Figure 2 8 A concentration step during magnetic particle processing Thermo Scientific KingFisher Flex User Manual 19 Functional Description Working with a magnetic rod 20 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Delivery check Thermo Fisher Scientific Unpacking gt D Chapter 3 Installation This section covers the relevant procedures to be carried out on receipt of the instrument Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective antistatic plastic wrapping until the ambient temperature has been reached Unpack the KingFisher Flex instrument and accessories
45. eywords for Thermo Fisher Scientific web pages Chapter 10 References bead based beads biopanning cell enrichment cell purification cell separation DNA DNA extraction DNA immobilization DNA isolation DNA purification DNA strand separation DNA template double stranded DNA genomics DNA genomics GST tag proteins His tag proteins immunomagnetic separation immunoprecipitation KingFisher magnetic beads magnetic capture magnetic microspheres magnetic particles magnetic separation microspheres nucleic acids oligo dT particles PCR product phage display post PCR purification primer processing processor protein purification proteomics purification RNA mRNA recombinant protein purification RT PCR separation silica coated particles streptavidin coated beads streptavidin coated particles Superparamagnetic beads Superparamagnetic microspheres Thermo Fisher scientific Thermo Scientific Thermo Scientific KingFisher Flex User Manual 85 References Literature Literature 86 Thermo Scientific KingFisher Flex User Manual Aasheim H C Deggerdal A Smeland E B and Hornes E 1994 A simple subtraction method for the isolation of cell specific genes using magnetic mono disperse polymer particles Biotechniques 16 4 716 721 Boom R Sol C J A Salimans M M M Jansen C L Wertheim van Dillen P M E and van der Noordaa J 1990 Rapid and simple method
46. fications Capacity Collection efficiency of the particles indoor use Magnetic particle size Magnet rods Plate types disposable Recommended filling volume Tip combs polypropylene disposable Heating block temperature Heating block accuracy Keyboard Display Type 711 CE mark CSA 96 samples run gt 95 KingFisher 96 plate neutral wash buffer containing detergent 2 8 um particles 3 collections RT ca gt 1 um 24 or 96 in one frame Interchangeable KingFisher Flex heads 24 96 well plates KingFisher 24 deep well plate 200 ul 5 mL Microtiter deep well 96 plate 50 1000 ul KingFisher 96 plate 20 200 ul PCR plate 20 100 ul skirted 24 96 in one frame for KingFisher 24 deep well plate for Microtiter deep well 96 plate for KingFisher 96 plate for PCR plate From 5 C above ambient temperature to 115 C 1 C up to 80 C 2 C up to 115 C instrument in RT START PAUSE STOP OK TURNTABLE ROTATING CLOCKWISE TURNTABLE ROTATING COUNTERCLOCKWISE four cursor keys LCD This section describes the safety specifications for the KingFisher Flex instrument KingFisher Flex bears the following markings 100 240 Vac 50 60 Hz 150 VA KingFisher Flex conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 S
47. for any damages whatsoever arising out of the use or inability to use this product Warranty statement Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of one year from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions The warranty is invalid if products have been misused or abused For the warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the type listed above e Refurbished products that are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for an
48. for purification of nucleic acid J Clin Microbiol 495 503 Boom W R Adriaanse H H Kievits T and Lens P F 1993 Process for isolating nucleic acids United States Patent number 5 234 809 Che S and Ginsberg S D 2004 Amplification of RNA transcripts using terminal continuation Laboratory Investigation 84 131 137 Coche T Dewez M and Beckers M C 1994 Generation of an unlimited supply of subtracted probe using magnetic beads and PCR Nucleic Acids Res 22 1322 1323 Coty C 2002 Purifying Nucleic Acids Genomics amp Proteomics 2 1 35 38 Cudjoe K S 1999 Inmunomagnetic particle based techniques Overview In Eds Robinson R K Batt C A and Patel P D Encyclopaedia of Food Microbiology Academic Press London UK Pp 1088 1094 Ginsberg S D 2002 High quality mRNA extraction with KingFisher for molecular fingerprinting Focus 1 5 Ginsberg S D and Che S 2002 RNA Amplification in Brain Tissues Neurochem Res 27 10 981 992 Hawkins D J O Connor Morin T Roy A and Santillan C 1994 DNA purification and isolation using a solid phase Nucleic Acids Res 22 4543 4544 Hornes E and Korsnes L 1990 Magnetic DNA hybridisation properties of oligonucleotide probes attached to superparamagnetic beads and their use in the isolation of poly A mRNA from eukaryotic cells Genet Anal Tech Appl GATA 7 145 150 Jakobsen K S
49. g The range of movement of the head and the magnets is limited if the magnets are not attached to the magnet lift This is to prevent running a purification sequence without the magnets The command is not recordable This error is reported if a nonrecordable command is received when recording Is on Not enough memory available for operation For example maximum exceeded in recording Description Timer already expired This warning is reported if a wait for a timer WAI command is executed and the timer has already expired A troubleshooting guide for the KingFisher Flex instrument is presented Table 8 9 Actions taken against error messages and warnings Action Contact authorized technical service Switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service Switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service Contact authorized technical service Contact authorized t
50. gFisher Flex heads Do not hold the KingFisher Flex heads close to a PC display since this may cause damage to the display Do not use metal tools when handling KingFisher Flex heads Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field A Q6 How can the KingFisher Flex heads be changed A6 By running the Change_magnet protocol under the Maintenance menu using the up and down cursor keys Q7 Can I concentrate the sample during the run A7 Both deep well plates and KingFisher 96 plates can be used during the same run Therefore it is possible to start the processing by using larger volumes in a deep well plate and elute the purified sample to a smaller volume in a KingFisher 96 plate Q8 How does the heating block work A8 The heating block is located inside the instrument and can be used automatically during the protocol All KingFisher Flex plates can be heated using specially designed interchangeable heating blocks Any number of heating steps can be added to the protocol During the protocol when the protocol enters the heating step the plate is automatically moved to the dedicated heating position for heating After the heating step the protocol continues automatically to the next step Note No cooling steps can be add
51. have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 89 Certificate of Decontamination 90 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Appendix B Thermo Scientific KingFisher Flex Feedback Form Instrument Thermo Scientific KingFisher Flex O Instrument serial no PURCHASED BY Company Institute Department Address Tel Fax PURCHASED FROM Distributor Address Tel Date of delivery Internet home page Date of purchase Your application area D O M O Mrs 0OO Ms 0O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above expectations Instrument installation o O Ease of use oO O Flexibility oO oO User manual oO oO software o O Customer support oO o
52. he Maintenance menu The values shown in the options Maintenance menu remain in the instrument memory and are instrument specific not protocol specific Device information The device information shows the name of the instrument the internal software version and the serial number of the instrument unit Go to the Maintenance menu Not active English OK Exit Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 47 Routine Operation Instrument options Press OK to exit Computer interface There are two types of computer interfaces available RS 232 and USB RS The instrument will automatically connect to the interface that is first used after the power is switched on Go to the Maintenance menu Select the Computer interface row English p a 3 isa This selection also has to be done when you use the instrument through BindIt Software Refer to Chapter 2 Installing the Software in the BindIt Software User Manual Cat no NO7974 s 5elect OK Accept AV Use the Up and Down arrow keys to select the computer interface in use Not active will reset the computer interfaces Press OK Language You can set the language of the internal software con Go to the Maintenance menu Select the Language selection row ad 48 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Routine Operation Instrument options j raed 2 alt
53. he transport lock of the heating block is refitted according to Figure 3 27 Screw the transport lock screw clockwise Figure 3 27 Figure 3 30 shows both the transport locks refitted Figure 3 27 Refitting the transport lock of the heating block B How to refit the To refit the transport lock of the tip comb holder follow these steps transport lock of the tip comb holder 1 The transport lock of the tip comb holder is refitted according to Figure 3 28 through Figure 3 29 Press the transport lock from both sides and insert the two eccentric pins back into place into their respective holes Figure 3 28 Caution Ensure that the power is switched off A 36 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Installation How to pack the instrument for transportation r port Lock Use CAUTION e Trans Remoy Before wn a Z y f Figure 3 28 Fitting the transport lock of the tip comb holder A 2 Press the tip comb holder transport lock ends together with both hands and insert all the way down to lock position Figure 3 29 Note that the turntable can move slightly although the transport lock is fitted ae Figure 3 29 Fitting the transport lock of the tip comb holder B Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 37 Installation How to pack the instrument for transportation at o o a CAUTION ve Transport Before Use
54. ient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation A Thermo Scientific KingFisher Flex User Manual 73 Technical Specifications In conformity with the requirements 74 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Thermo Fisher Scientific O amp As Chapter 7 Frequently Asked Questions QI What does the KingFisher Flex system do A1 Thermo Fisher Scientific now offers a complete KingFisher Flex system the magnetic particle processor for the purification and processing of proteins DNA RNA and cells in a 24 or 96 well format The processor handles particles automatically according to the preloaded purification protocols For more information on these applications contact Thermo Fisher Scientific Oy Q2 What plates can be used with the KingFisher Flex A2 The KingFisher Flex is compatible with four different plate types KingFisher 24 deep well plates Microtiter deep well 96 plates KingFisher 96 plates and PCR plates Each plate type has an optimized KingFisher Flex head and tips Of the four plates the KingFisher 96 plate can be used with all three kinds of magnets for example KingFisher 96 plates ca
55. ilated e Never spill fluids in or on the equipment Caution The KingFisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the KingFisher Flex heads Do not hold the KingFisher Flex heads close to a PC display since this may cause damage to the display Do not use metal tools when handling KingFisher Flex heads A Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field A This section describes the installation setups that you must carry out before operating or relocating the instrument The instrument comes with two transport locks Figure 3 9 the tip comb holder transport lock and the heating block transport lock Remove both the transport locks Make sure both the transport locks are released before you put the instrument into operation Thermo Scientific KingFisher Flex User Manual 23 Installation Installation setups 24 Thermo Scientific KingFisher Flex User Manual To remove both the transport locks follow these steps Tip comb holder transport lock Figure 3 9 Kingfisher Flex transport locks fitted 1 To remove the transport lock of the tip comb holder take firmly hold of
56. in M Nordentoft S Bang D D Pedersen K and Madsen M 2003 Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark J Appl Microbiol 94 929 935 M kinen J Marttila H and Viljanen M K 2001 Automated purification of Borrelia burgdorferi s 1 PCR products with KingFisher magnetic particle processor prior to genome sequencing Journal of Magnetism and Magnetic Materials 225 134 137 Portelius E Tran A J Andreasson U Persson R Brinkmalm G Zetterberg H Blennow K and Westman Brinkmalm A 2007 Characterization of amyloid B peptides in cerebrospinal fluid by an automated immunoprecipitation procedure followed by mass spectrometry Journal of Proteome Research 6 4433 4439 Robino C Barilaro M B Gino S Chiarle R Palestro G and Torre C 2006 Incestuous paternity detected by STR typing of chorionic villi isolated from archival formalin fixed paraffin embedded abortion material using laser microdissection Journal of Forensic Sciences 51 90 92 Rudi K Kroken M Dahlberg O J Deggerdal A Jakobsen K S and Larsen F 1997 Rapid universal method to isolate PCR ready DNA using magnetic beads Bio Techniques 22 506 511 Sinclair B 1998 To bead or not to bead Applications of magnetic bead technology Scientist 12 16 19 Tu S Golden M Cooke P Paoli G and Gehring A 2005 Detection of Esch
57. ing magnetic particles Releasing magnetic particles Washing magnetic particles Incubation Changing the volume during the magnetic particle processing 18 Thermo Scientific KingFisher Flex User Manual Working with magnetic particles can be divided into five separate processes e collecting magnetic particles e releasing magnetic particles e washing magnetic particles e incubation concentration During the collection of the magnetic particles the magnetic rod is fully inside the tip The magnetic rods together with the tip comb move slowly up and down in the plate and the magnetic particles are collected onto the edge of the tips The magnetic rods together with the tip comb having collected the magnetic particles can be lifted out of the plate and transferred into the next plate After collection of the magnetic particles the magnetic rods together with the tip comb are lifted from the plate the magnetic rods are lifted off and the tip comb is lowered into the next plate containing a reagent Magnetic particles are released by moving the tip comb up and down several times at considerably high speed until all the particles have been mixed with the substance in the next reaction Washing the magnetic particles is a frequent and an important processing phase Washing is a combination of the release and collection processes in a plate filled with washing solution To maximize washing efficiency the magnetic rods tog
58. ion tests and adjustments have been completed Thermo Fisher Scientific Installation How to pack the instrument for transportation It is recommended that you carry out a check run using a demo protocol to verify proper instrument operation Run the check protocol Check_96dw_tip Check_KF96_tip Check_pcr_tip or Check_24dw_tip under the Maintenance menu according to the KingFisher Flex head and plastics you are using If the check is all right proceed with your own runs How to pack the This section describes how to refit both the transport locks the instrument for transportation transport lock of the heating block and that of the tip comb holder How to refit the To refit the transport lock of the heating block follow these steps transport lock of the heating block 1 Remove the heating block in the following way First choose the Change_heatblock protocol Then press START Remove the heating block by pulling it out Press the START key when the task has been completed 2 Switch off the power 3 Take the transport lock screw from its storage site 4 Insert the transport lock screw into the label and refit the transport lock of the heating block into its place Figure 3 26 Ae ll w Figure 3 26 Refitting the transport lock of the heating block A Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 35 Installation How to pack the instrument for transportation 5 T
59. n refer to Chapter 10 Ordering Information For more For PC software related issues refer to the Thermo Scientific BindIt info rm ation Software User Manual Cat no N07974 For the latest information on products and services visit our websites at http www thermo com http www thermo com kingfisher In our efforts to provide useful and appropriate documentation we appreciate your comments on this user manual to your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 3 About This User Manual safety symbols and markings Safety symbols and markings Safety symbols and markings used on the KingFisher Flex Sc HE ORO 4 Thermo Scientific KingFisher Flex User Manual These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated The following symbols and markings appear on the type label and the instrument itself Power ON a Power OFF a Warning Hot surface risk of burns A Warning Risk of body parts hair jewelry or clothing getting caught in a moving part A Serial number a Catalog number a Date of manufacture a Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A A black label with the following text Figure 2 4
60. n be used together in a protocol with either Microtiter deep well 96 plates or PCR plates Q3 Can I use plates from other manufacturers A3 No it is highly recommended to use plates listed in Table 9 11 on page 83 These plates are specifically designed to be used with KingFisher Flex tip combs to attain maximal performance Plates from other manufacturers may not be compatible with the KingFisher Flex heating blocks They may also cause unexpected problems such as cross contamination due to the divergent well volume and bottom height of the plate Q4 Will the magnet get weaker If so how long can they be used A4 The KingFisher Flex magnets are made of material that is very stable The magnetic field will not get weaker However extreme mechanical force or heating can cause damage to the magnets Caution It is very important to keep the KingFisher Flex heads away from each other and other magnets at all times Clashing of the magnets together may cause serious damage to the magnets A Thermo Scientific KingFisher Flex User Manual 75 Frequently Asked Questions Q amp As 76 Thermo Scientific KingFisher Flex User Manual Q5 How strong are the magnets Can they for example disturb some sensitive equipment A5 The KingFisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the Kin
61. n the documentation The device shall be operated only with software specifically designed for the device Never open any other covers of the KingFisher Flex than the see through lid or the sliding door Figure 2 3 while the instrument is plugged into a power soutce Never force a microplate onto the instrument The KingFisher Flex is intended for laboratory research use only Observe proper laboratory safety precautions such as wearing protective clothing and following approved laboratory safety procedures Preventative maintenance instructions should be followed closely to keep the instrument in the best condition for maximum reliability A poorly maintained instrument will not give the best results Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field A Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Thermo Fisher Scientific Contents ene UNS CS casey crenata nenataea dias thc aseeameradeeres A E 3 How to use this user manual seeneseeneseensssorssecssesreseeresecrssecrsesrese 3 Formore morna oen a alata a 3 Satety symbols anid Markings iasi e a apeancdes 4 Safety symbols and markings used on the KingFisher Flex 4 Warning and other markings used in the documentation 5 Ins
62. nes 15 ODES A E E A E E A E E AA A E A T a 8 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 8 Chapter 9 Chapter 10 Appendix A Appendix B Contents Troubleshooting Guide a snsnnnsununununununnnnnnnnnnnnnnnnnnnnnnnnnnununnnnnunnnnnnnnnnnnnn 79 Error messages and Wal DINE Sano a a 79 Troubleshooting COldE sapeni ainan a E 80 Service teguest Protocole o ana E R 81 Certificate of Decontamination cccccccecccscceccascceccaccascceccasceeceass 82 Ordering InformatiOn ccscssscsssessseesssesseseseesesesesseseeseseesesseseeseeaes 83 Kine ris hier Plex caivisirestcay terete irises A eaten ese 83 List of accessories and consumables c csccsecaecceccecceecceccascaecenses 83 Liitot Spake Pals iendane a eas a a 84 Referentes arisia a a a 85 Keywords 1OP WED Paa S rererere TE 85 lrer a a a a aa aa 86 Certificate of DeContamimatiOn cccccceccceecssecsseecssesseessseeceseesseeeseees 89 Thermo Scientific KingFisher Flex Feedback Form 0000008 91 Thermo Scientific KingFisher Flex User Manual 9 Figures 10 Thermo Scientific KingFisher Flex User Manual Figures Figure 1 1 KingFisher Flex magnetic particle processor 13 Figure 2 2 Kingfisher Flex front view without see through lid 0 0 15 Figure 2 3 KingFisher Flex front view with see through lid and plates 15 Figure 2 4 KingFisher Flex b
63. ntamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is a risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another or before sending it to service Decontamination is not required for the proper functioning of the instrument Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 Warning The decontamination procedure should be performed by authorized trained personnel wearing disposable gloves protective glasses and clothing in a well ventilated room A 1 Prepare the decontaminant 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer Thermo Fisher Scientific Thermo Fisher Scientific Packing for service A 10 Maintenance Packing for service Empty the turntable Switch OFF the power and disconnect the mains supply cable Figure 3 14 Disinfect the outside of the instrument using a cloth dampened with 70 ethanol Place the instrument in a large plastic bag Ensure that the see through lid has been removed Place a cloth soaked in the prepared solution into the b
64. of 18 77 release of 18 wash of 18 magnetic rod 2 13 16 17 18 33 59 65 72 76 77 93 Thermo Scientific KingFisher Flex User Manual 95 maintain 63 68 maintaining the instrument 3 27 33 35 41 44 48 49 50 51 63 76 maintenance 3 6 27 33 35 41 44 48 49 50 51 52 63 68 76 81 protocol 44 51 52 navigating 41 0 operational check 34 39 81 ordering information 3 83 Other 43 47 P packing 21 22 67 69 81 instructions 21 67 list 21 22 materials 69 PCR 28 29 31 32 53 54 59 61 72 75 83 85 86 87 93 plate 28 29 31 32 59 61 72 75 93 plate 6 13 15 16 17 18 28 29 30 31 32 39 40 52 53 54 55 56 57 58 59 60 61 66 72 75 76 77 84 93 station 16 55 56 93 processing head 16 Protein 43 47 protocol 13 16 27 28 33 35 40 43 45 46 47 48 51 52 55 56 59 60 75 76 79 80 81 86 refitting the transport lock of the heating block 26 35 refitting the transport lock of the tip comb holder 25 26 36 refitting the transport locks 25 26 35 36 S samples 13 14 16 23 59 66 72 76 77 93 96 Thermo Scientific KingFisher Flex User Manual see through lid 6 15 17 27 34 39 55 58 59 67 service 39 62 66 67 68 80 81 83 contracts 68 request protocol 81 shield plate 27 29 30 31 58 63 66 79 80 93 shutting down 61 sliding door 6 17 34 39 55 58 59 specifications
65. open Figure 2 3 KingFisher Flex front view with see through lid and plates Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 15 Functional Description KingFisher Flex magnetic particle processor Back side view KingFisher Flex magnetic particle processor 16 Thermo Scientific KingFisher Flex User Manual The back view of the KingFisher Flex instrument is shown in Figure 2 4 and the side view in Figure 2 5 Mains power Cooling air inlet Type label USB port Warning marking supply connector Cooling air outlet Serial port RS 232C ON OFF switch Figure 2 4 Kingfisher Flex back view Figure 2 5 Kingfisher Flex side view The KingFisher Flex Figure 2 6 has room for eight plates The tip combs are compatible with the plates During the individual steps the plates are kept stationary and the only moving assembly is the processing head with tip comb and magnetic rods The head consists of two vertically moving platforms One is needed for the magnetic rods 24 or 96 pieces and the other one for the plastic tip comb Up to eight plates can be simultaneously on the turntable However during one sample processing the protocol enables the use of more than eight plates in total One tip comb contains 24 or 96 tips used for processing 24 or 96 samples at a time Before starting the magnetic particle processing via the keypad and display the samples and reagents are dispensed into the plates
66. page 45 Default Selecting the protocol on page 44 DNA RNA Cell Protein Other Device information on page 47 Computer interface on page 48 Language on page 48 Buzzer on page 49 Maintenance protocol on page 50 Thermo Scientific KingFisher Flex User Manual 43 Routine Operation Using KingFisher Flex PC software Using internal software Factory User protocols Selecting the protocol 44 Thermo Scientific KingFisher Flex User Manual The operation of the KingFisher Flex magnetic particle processor can also be controlled by an external computer and run on BindIt Software In addition to the KingFisher Flex internal software features you can also download protocols to the instrument or back up protocols from one instrument and transfer them to another For more information refer to the BindIt Software User Manual Cat no N07974 This section describes procedures related to the KingFisher Flex internal software This section describes the factory user set protocols They are found in the Factory protocols User protocols menu Factory protocols are protocols which are preloaded into the instrument internal memory in the factory for example demo protocols User protocols are protocols which are made and transferred using BindIt Software Go to the Factory protocols User protocols menu Select for example the DNA RNA row DNA RNA Cell Protein Other
67. rn your instrument or other items e Enclose the return goods authorization number RGA given by your Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s technical service department Refer to General specifications on page 71 for details on storage and transportation temperatures Service Itis recommended to maintain and service the instrument regularly every 12 months on a contract basis by the manufacturer s trained contracts ee i i n service engineers This ensures that the product is properly maintained and gives trouble free service Contact the Thermo Fisher Scientific technical service department for more details Disposal of the If the KingFisher Flex has to be disposed of follow the guidelines below instrument A Warning Decontaminate the instrument before disposal Refer to Decontamination procedure on page 66 and Certificate of Decontamination on page 82 about decontamination A Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The procedures vary by country Pollution degree 2 see Safety specifications on page 72 Method of disposal Electronic waste Contaminated wa
68. s Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field A To insert KingFisher Flex heads choose the Change_magnet protocol under the Maintenance menu by using the up and down cursor keys Then press START Insert the KingFisher Flex head so that the three pins shown with black arrows slot into place Figure 3 23 and Figure 3 24 When you remove the KingFisher Flex head be careful not to damage the magnet rods against the tip comb holder frame Figure 3 23 Inserting the KingFisher Flex head Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 33 Installation Operational check See through lid Operational check 34 Thermo Scientific KingFisher Flex User Manual Figure 3 24 KingFisher Flex head for KingFisher 24 deep well plate fitted During operation the sliding door can be open or closed or the whole see through lid can be absent Figure 3 25 The closed door protects the processing against environmental contamination see through lid Sliding door open Figure 3 25 See through lid fitted First switch the instrument ON The instrument performs initialization tests and adjustments The display quickly shows the internal software version This happens when the initializat
69. s and the processor and to keep the best performance at the most efficient level Q15 Is it always compulsory to use the transport locks A15 The transport locks are only necessary when relocating the instrument Q16 What should you do if the tip loading does not succeed A16 Try first to manually stretch the tips both lengthwise and width wise to level the tip comb Thermo Scientific KingFisher Flex User Manual 77 Frequently Asked Questions Q amp As 78 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Chapter 8 Troubleshooting Guide properly A Note Do not use the instrument if it appears that it does not function Note that the instrument does not verify the logic flow of the received commands Error messages When an error is detected the current operation is terminated After an and warnings error it is best to abort the current run and restart from the beginning after the problem is fixed The KingFisher Flex internal software has the following error messages and warnings Table 8 8 Table 8 8 Error messages reported Code Error message 0 No error 1 The command was not recognized as a valid command 2 The tip comb holder lifting mechanism is out of position 3 The turntable rotating mechanism Is out of position 4 The magnetic head holder lifting mechanism is out of position 5 The heating block lifting mechanism is out of position 6 The shield plate turning mechanism Is
70. ste Infectious waste 68 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Thermo Fisher Scientific Maintenance Disposal of the instrument WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEERoHS A Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative Thermo Scientific KingFisher Flex User Manual 69 Maintenance Disposal of the instrument 70 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific General specifications Thermo Fisher Scientific Chapter 6 Technical Specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program The general specifications are presented in Table 6 6 Table 6 6 General specifications General specifications Overall dimensions instrument transport package Weight instrument incl
71. switched OFF 2 The shield plate has to be installed prior to use of the instrument Use a hexagonal screwdriver Allen key to fasten the two screws Figure 3 18 Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 29 Installation Installation setups Figure 3 18 Shield plate prior to fitting 3 First insert the shield plate into its slot Figure 3 19 Figure 3 19 Inserting the shield plate 4 When fastening the two plate retaining screws Figure 3 20 be careful not to drop them inside the instrument Figure 3 21 shows the shield plate installed 30 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific KingFisher Flex heads Thermo Fisher Scientific Interchangeable A Installation Installation setups le U Siy p Figure 3 20 Fastening loosening the shield plate Figure 3 21 Shield plate fitted There are four kinds of interchangeable KingFisher Flex heads available for KingFisher 24 deep well plates Microtiter deep well 96 plates KingFisher 96 plates and PCR plates The KingFisher Flex heads all have corresponding disposable plastic tip combs Table 3 2 Note that KingFisher Flex heads do not fit into the KingFisher 96 and vice versa Caution Do not place the KingFisher Flex heads on top of the instrument or any metal surfaces Keep the KingFisher Flex heads always in their respective plastic boxes when not in us
72. th dampened in a warm mild detergent solution will be sufficient Clean the outside of the instrument and the turntable with clean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary Although the KingFisher Flex is constructed from high quality materials you must immediately wipe away spilt saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose the surfaces to concentrated acids or concentrated alcohols for prolonged periods of time as damage may occur A Clean the display areas with a mild laboratory detergent The keypad has a wipe clean surface Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces are contaminated with biohazardous material a mild sterilizing solution should be used A Caution Do not autoclave any part of this instrument except the shield plate A Keep the turntable surface clean to avoid dust and dirt entering into the instrument Clean the turntable surface at least once a week using a soft Thermo Scientific KingFisher Flex User Manual 63 Maintenance Regular and preventive maintenance cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol The turntable can be detached for cle
73. tiate the run To confirm a performed step in the protocol for example plate loading or removal To pause terminate the processing step In short STOP paused START the instrument continues after a Pause step STOP paused STOP the processing is terminated Thermo Fisher Scientific Display icon lt gt Navigating AVG Thermo Fisher Scientific Routine Operation Control panel To pause the run It will pause at the end of the ongoing processing step To rotate the turntable clockwise TURNTABLE ROTATING CLOCKWISE button To rotate the turntable counterclockwise TURNTABLE ROTATING COUNTERCLOCKWISE button The liquid crystal display is a 240 x 360 pixel display The main view in the display is shown in Figure 4 32 7 Menu Main view row DNA RNA Cell Protein Other a E ETE info text bar Figure 4 32 Main view on the display of the KingFisher Flex There are three menus in the KingFisher Flex user interface Factory protocols User protocols and Maintenance In routine use you mainly navigate in the Factory User protocols menu In advanced level options there is one extra menu Maintenance You can navigate between these three menus using the Left and Right arrow keys The main view row is either colored active or uncolored different colored inactive All the descriptive icons used in the main view are shown in Table 4 3 below The info text bar shows explana
74. tory information on how to proceed and which keys to use This section visualizes navigation in the KingFisher Flex user interface The main view changes according to the selections you make either with the Up or Down arrow keys or the OK button The available buttons and their function are shown on the info text bar Thermo Scientific KingFisher Flex User Manual 41 Routine Operation Control panel Active item Cell Protein Other OK Protocol selection DNAIRNA Cell Protein Other Select START Run OK Details Factory protocols 42 Thermo Scientific KingFisher Flex User Manual The color of the items for example the icon and main view row in the main view changes when they are selected active inactive To move from one menu to another make sure you are in the main view of one of the menus and use the Left and Right arrow keys The main views of each menu tab are shown below DNAIRNA Cell Protein Other Select START Run OK Details User protocols Not sy Bt yg Not defined English ee OK Device information Maintenance Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Control panel Table 4 3 Icons in the main view Function Icon rm Setting the default protocol on page 45 e a Last used Default Selecting the protocol on page 44 DNA RNA Cell Protein Other Setting the default protocol on
75. trument safety and guidelines for USC seeseceeseeeeeeeeeeeeeeeeeeaeees 6 Introduction to the KingFisher Flex ccssesssssessesesesnnnres 13 LACES CIS 5 5 eastern a a a 13 Piimeiple Ol operation sengen tomes otucentidestares omnes tunedin 13 Advantages of using KingFisher Plexo eee eeeeeeeseeeeeneeeeeneeeeeaeeees 14 Functional Description ccsscsssscsessssseesseseeseseseeeeeeseseeoeseseeennseeens 15 lnstroament Tay OU cosas inn r N 15 POE onana aaa a A 15 Back l side view tdendosseisencoeaueuiniae ds eeutwdaseuvivosuseebSbaivdancoetoesvandeswassosuce 16 KingFisher Flex magnetic particle processor ceesecceeseeeeeseeeeenees 16 Principle of magnetic particle processing eeseeeeseeeeeneeeeeneeeeeaees 17 Working with a magnetic rod ceeeeseceesseeeeeseeeeeseeeeseeeesaneseeaeeens 18 Collecting mae Mee PANIC eS messinai 18 Releasing magnetic particle ieissa anoe 18 Washing magnetic particles jicisiiestnevcieiicavncesnentee 18 Tacuba o enea T E 18 Changing the volume during the magnetic particle processing 18 istallat OD ana a Givaabce tata thea sare sama chun tcasaxsoraecesatativens uaxeuts 21 Deler CCC hha ss sseetaewistet agate a a a 21 WTAE NS serenti niei E eetouseennee 2i Checkine deliye orini aiie E ee E E nner 22 Requien Seen n A 22 Precautions and limitations cccecccsseeccceseccceeccecssccccseccceesccenses 23 Psalliota p nier n n a E E O OE 23 Releasing the transp rt lockS 22
76. ubpart B Class B July 2004 2002 96 EC Waste of Electrical and Electronic Equipment 72 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Thermo Fisher Scientific Technical Specifications In conformity with the requirements Safety performance EN 61010 1 2001 Ed 2 including US and CA National differences EN 61010 2 010 2003 Ed 2 EN 61010 2 101 2002 Particular Requirements for In Vitro Diagnostic IVD Medical Equipment The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply 10 from nominal fluctuations Installation category ll according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected trans
77. uleprotoc Olav claro tia A 45 TS HUTTE EO PLIOIS irea EEE I 47 Device IDOM 1806 as E E ae ere Neer 47 Computer m aCeee e R E camara 48 Lano uae o r RE T E 48 DUZZO ienna E E E AOR E VO 49 Maintenance PrOtOCO kestirip E 50 Howto hande tip COMI S diece an N 51 FLOW O SCA orsa EN E E O eee ere cre 54 Changing the heating DloCkancrcorcnci neis iie ea E 58 SMELO Diere E verte eee ieee 60 Emercency situatio NS raei EE AAE 6l Chapter 5 Mainte aiit e nrnna aa aAa anaa adia ia 63 Regular and preventive maintenance sesssesssessssesssesseressressereseress 63 Howto clean the turnta Die asenin o NR 63 How to clean the magnetic rods sssssesssesesesesssesssressressersssressees 65 How 10 Clean tne Shield paie seca wien estnenaee eceaalie 66 Disposalof material ernen era estalcie ela anew acca veseeeteatentna 66 Decontamination procedive eeste ia a 66 PACKING TOR SERV CE Keressen ne E RE 67 SOVICE CONTAC Saaai a Pot attat mache tine Roea Mantis 68 Disposalsor the instrument vcudctasci easel nateeieahtcieiee arenes 68 Chapter 6 Technical Specifications sssssessssssssssssssssssrsesesssssesersesesssssstaraess 71 Grene Las PECINCAT ONS aosriisare tiene atersmuntenioceut teeta eis 71 Performance specilicationS tisini n i E A 71 Salely SPEC CONS n E A eae T2 In conformity with the requirements sssssesseessseressssrsssrrssseresses 72 Chapter 7 Frequently Asked Questions ccccssssssssssssssesseessesssseennseennseennseso
78. ve packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy When you set up your KingFisher Flex avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight or UV light draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables and reagent bottles e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make sure the ambi
79. well 96 plates KingFisher 96 plates and PCR plates Figure 4 48 and Table 4 5 Heating block Plate Figure 4 48 Four different heating blocks available Table 4 5 Heating blocks vs plate types KingFisher 24 Microtiter deep KingFisher 96 PCR plate deep well plate well 96 plate plate skirted Heating block for KingFisher 24 deep X well plate Heating block for Microtiter deep well X 96 plate Heating block for KingFisher 96 plate X X Heating block for PCR plate X A A Shutdown 60 Thermo Scientific KingFisher Flex User Manual Warning The heating block surface can be hot whereby there can be risk of burns A Caution The heating block is specifically designed for the plates listed below to ensure even heating during the sample process Figure 3 17 Using other plates than those recommended may damage the instrument and diminish the application performance A To shut down the KingFisher Flex follow the steps below 1 Switch the KingFisher Flex off by pressing the power switch Figure 2 4 at the bottom left of the back side panel of the instrument into the OFF position Warning Discard the plastic tip comb onto the plate Dispose of all tip combs as biohazardous waste Remove any plates still in the instrument Dispose of all microplates as biohazardous waste A Thermo Fisher Scientific Thermo Fisher Scientific Emergency situations Routine Oper
80. y loss or damage arising out of or in connection with the use of the product or other indirect damages These warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer Consumables are not included in the warranty About This User Manual Intended USCS This user manual is written for the actual end user for example research scientist or laboratory technician and provides information on the Thermo Scientific KingFisher Flex including the installation and operating instructions Read the manual in its entirety before operating the instrument How to use this This user manual is designed to give you the information to user manual e Review safety precautions e Install the KingFisher Flex e Use the KingFisher Flex in routine jobs the processing step e Perform basic cleaning and maintenance procedures e Troubleshoot the instrument performance This user manual also describes all the features and specifications of the KingFisher Flex hardware and onboard software Refer to Chapter 7 Technical Specifications In Chapter 4 Routine Operation you find explanations of the processing principles and procedures and on how to use the KingFisher Flex internal software In Chapter 9 Troubleshooting Guide you find explanations of error and warning messages and a problem solving guide The user should be familiar with the contents of Chapter 5 Maintenance For ordering informatio

KingFisher Flex User Manual

Contents

Download Pdf Manuals

Related Search

Related Contents