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5. Agilent 7100 Capillary Electrophoresis System User Manual 45 2 Site Requirements and Specifications 12 35 kg 350 x 510 x 590 mm x x 100240 VAC 10 50 60Hz 5 350 VA 300 W 1024 B TU 540 C 40 70 31 80 BEZEL 2 000 m 0 5 bar 50 2 12 bar IEC Il ERE 2 CSA UL CEC
6. 50 Agilent 7100 Capillary Electrophoresis System User Manual 14 Site Requirements and Specifications 2 Hain hot CBE x AR x D I TEC CSA UL 7 y 35 kg 350 x 510 x 590 mm 100 240 VAC 10 50 Hz 60 Hz 5 350 VA 300 W 1024 BT U h 5 40 C 40 C 70 C 40 F 158 F 31 C 80 2000 m 0 5 bar 50 C 2 12 bar IL E ho CEC EME H
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13. Agilent 7100 Capillary Electrophoresis System User Manual mk CE p 15 bar PTFE Agilent 7100 51 2 Site Requirements and Specifications 15 Agilent 7100 Windows XP SP3 Agilent ChemStation LAN CAN RS 232C 0V 1V APG USB GLP EMF 52 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System M User Manual 000 ee 3 eve
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15. o 1 Agilent 7100 Capillary Electrophoresis System User Manual 251 10 Appendix Setting Up a Test Sample Run 252 This instrument check demonstrates that the instrument is correctly installed and connected Please use a fused silica capillary with 40 cm effective length and 50 um inner diameter from the HPCE start up capillary kit part number G1600 68700 with the green alignment interface part number G7100 60210 The instrument should be in CE mode Load chemicals from the Installation Qualification Chemical kit part number 5063 6514 according to the table below The Installation Qualification Chemical kit contains Buffer 20 mM borate pH 9 3 100 mL Test sample 4 hydroxyacetophenone 1 mM 3 mL Conditioning solution 1 0 N sodium hydroxide 100 mL Table34 Solution for Checkout Run Location Solution Volume Vial 1 1 0 N sodium hydroxide 500 uL Vial 2 Water 200 uL Vial 4 Buffer 500 uL Vial 5 Buffer 500 uL Vial 6 Buffer 500 uL Vial 7 Test sample 500 uL Vial 8 Water waste 200 uL Choose the menu item Method New method to prepare a method with the parameters given below Save the edited method under a new name Choose the menu item RunControl gt Sample Info and enter the operator name directory and file name Enter Vial loc
16. CEtp 15 bar PTFE Agilent 7100 46 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 13 Agilent 7100 del Agilent ChemStation Windows XP SP3
17. LAN 0 1V CAN RS 232C APG Ready Start Stop Shut dom USB GLP EMF Agilent 7100 Capillary Electrophoresis System User Manual 47 2 Site Requirements and Specifications 7100 51 14
18. ER Et BEAR VE E EMC Agilent 7100 Capillary Electrophoresis System User Manual 49 2 Site Requirements and Specifications 2 5 cm 8 cm Agilent 7100 51 14
19. Using the Agilent 0 Capillary Electrophoresis System Capillary Electrophoresis Plus High Pressure CE p 97 DAD sj ce EX Instrument Curves a Vials In Replenishment Inlet Home 04 buffer Preconditioning p Outlet Home 05 buffer M shoes Oda AAA h r to Apply pressure to Inlet Inlet Home Vial let Outlet Add method vial High pressure flush Cassette Temperature Fin Not controlled o 120 bar o 200 High Voltage System eki V Enable High Voltage ea uA Voltage 00 KV positive Polarity BW Current 30 pA ME s Power 60 w Inlet Inlet Home Vial X Of Outlet Outlet Home Vial Y Low Current alarm limit 100 Apply pressure to Inlet r Apply pressure to Outlet Shine Posting Apply pressure to both As Injector No Limit Of 10 00 min 100 min Aopend iset dete Cearat Wow en copy Feste More om Injection Timetable Postconditioning Figure 40 Method setup with CE p mode activated 144 Agilent 7100 Capillary Electro r Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis Plus High Pressure CE p Figure 41 Parameters for high pressure flush Other parameters which can be set in the High pressure flush command menu include Applied voltage kV current uA or power W Duration s nlet vial and or outlet vial Agilent 7100 Capillary Electrophoresis System User Manual 145
20. Access the pre punchers Clean the electrodes Clean the pre punchers Clean the insulation plate e Reinstall the pre punchers Reinstall the insulation plate Reinstall the electrodes Follow the common safety regulations for laboratories when doing this task Wear safety glasses and rubber gloves Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Make sure you have disconnected the line power cord of the Agilent CE instrument before you proceed When If arcing or excessive current leakage is observed Parts required 12 mm hexagonal socket screwdriver included in the startup kit Screwdriver for slotted screws included in the startup kit Screwdriver for cross head screws included in the startup kit Wash bottle with isopropanol Wash bottle with water Compressed inert gas oil free Prepare the Agilent CE Instrument Prior to maintenance do the following 1 Make sure no analysis is running or will be started from the ChemStation 2 Start monitoring your CE instrument with the Lab Advisor 3 Select the Tools section in the instrument panel of the Lab Advisor 4 Select the CE control screen and then Maintenance positions These steps unload all lifters and move them to the lowest position With all lifters in this position the tray is freely movable A dialog reports which lifters were unloaded in case vials were in the system Turn off the Agilent CE instrument Disconnect the line p
21. Common functions Run synchronization through APG remote Error handling Diagnostic functions Or module specific functions Internal events such as lamp control filter movements Raw data collection and conversion to absorbance 176 Agilent 7100 Capillary Electrophoresis System User Manual Hardware Information 7 Firmware Updates Firmware updates can be done using the Lab Advisor Software PC and Firmware Update Tool with local files on the hard disk To replace or update the firmware 1 Connect to the instrument with Lab Advisor and refer to the section Firmware Update 2 Provide the firmware file on your local hard disk You might locate the file in any folder and provide the location by selecting browse to folder or put it to a folder specific for your instrument CE LC 3 Connectto the instrument select the file in a standard folder or browse to the respective folder Download it to the instrument While the firmware is downloaded to the instrument the status indicator LED on the front of the instrument front will blink in red 4 Disconnect the firmware update tool from the instrument You can find a detailed description in respective help files of the Lab Advisor The file naming conventions are PPPP_RVVV_XX dlb for example 7101A_U 06 23_0002 dlb PPPP represents the product number R the firmware revision VVV is the revision number and XX is the build number of the firmware Updates to main system can
22. For the CE Leak current nternal pressure Replenishment level pressure Vacuum Injection pressure External pressure Tray temperature Cassette temperature For the DAD Board temperature Optical unit temperature UV lamp anode voltage 106 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Detector Parameters The parameters for the detector are set in the DAD tab of the Method Setup dialog used in the Agilent ChemStation see Figure 20 on page 93 On the left hand side you can define the following settings Used signals detection and reference wavelengths bandwidths Peak width or detector response time Detector stoptime and posttime Signals Wavelengths and Bandwidths Detector parameters allow you to define the detector signals you want to acquire during the analysis and store in the data file For each signal the wavelengths and bandwidths of sample and reference wavelengths are defined It is recommended to do runs without using reference wavelengths to minimize baseline noise Reference wavelengths can help to compensate for signal drifts As reference wavelengths are usually measured at higher wavelengths the baseline noise is increased when using reference wavelengths Therefore it might be appropriate to record the two signals at the same wavelength one using the reference wavelength and one without The sign
23. CAUTION Damage to the instrument Damage may lead to a non operational instrument Do not lift the liquid handling module upwards outside the instrument The module is connected to the main frame by two flat ribbon cables and two flexible tubes When lifting it up the liquid handling module might come off the guiding rail and the connections might be affected Use a screwdriver to unscrew the slotted screws holding the liquid handling module in the instrument Take the electrolyte and waste bottles out of the cabinet Keep them connected and place them in front of the instrument Carefully pull the liquid handling module out from the main frame Pull steadily in the middle until you reach the end position Carefully avoid damaging the replenishment needle Agilent 7100 Capillary Electrophoresis System User Manual 201 8 Maintenance Cleaning the Electrodes Pre punchers and Insulation Plate 1 Front screws 2 Take out bottles 3 Push liquid handling out Figure 61 Accessing the liquid handling module 202 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Cleaning the Electrodes Pre punchers and Insulation Plate Accessing and Removing the Pre punchers Inlet and outlet lifts are located behind the sample tray in the rear of the liquid handling module The replenishment lift is located in the front on the right side under the replenishment needle Outlet lift Inlet lift R
24. Agilent 7100 Capillary Electrophoresis System User Manual 157 5 158 Using the Agilent 7100 Capillary Electrophoresis System CEC Control Using the CE Diagram High pressure may be applied independently of the method for capillary conditioning or cleaning by clicking the external pressure icon in the CE diagram see Figure 46 on page 152 This will cause a parameter setting field to appear see Figure 47 on page 154 From this field you may chose to apply pressure to the inlet outlet or both vials and also whether to simultaneously apply a voltage of 30 to 30 KV for a given time Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Running the CEC Analysis After setting up the operational parameters you can initiate the run from the Method amp Run Control menu by clicking Run Method or by clicking Start in the CE diagram Some other operational aspects should be noted when running CEC separations Capillaries Packed capillaries are very fragile and must be handled with great care Offline purging of the capillary using an LC pump may be necessary if air bubbles form within the capillary Buffers CEC buffers generally have lower concentrations 4 20 mM than conventional CE buffers These may also contain a larger proportion of organic modifier than is found in CZE or MECC buffers The currents generated are generally on the order of 2 10 uA for an applied field
25. Da 40 a 70 C Inferiore all 80 a una temperatura di 31 C Fino a 2 000 m Max 0 5 bar max 50 C 2 12 bar Categoria di installazione Il grado di inquinamento 2 Tutti i materiali sono riciclabili Diversi valori di tensione accettati Massimo Assenza di condensa Bagno d acqua Aria priva di olio o azoto Solo per uso all interno Per le applicazioni in modalit CEC elettrocromatografia capillare o CE p elettrocromatografia capillare con utilizzo opzionale di alta pressione possibile usare un sistema esterno per alimentare aria priva di olii o azoto a una pressione massima di 15 bar Insieme al prodotto viene fornito un adattatore maschio che si inserisce nell adattatore femmina dello strumento e tubi in PTFE Il Sistema per elettroforesi capillare Agilent 7100 concepito per operare in un tipico ambiente elettromagnetico nelle cui vicinanze non devono essere presenti trasmettitori RF come telefoni cellulari Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Specifiche delle prestazioni Tavola 9 Specifiche delle prestazioni del Sistema per elettroforesi capillare Agilent 7100 Tipo Specifica Commenti Dispositivi di sicurezza Controllo e valutazione dei dati Comunicazioni Funzioni GLP Ampia scelta di funzionalit diagnostiche identificazione e visualizzazione degli errori rilevamento di perdite di corrente limite di corr
26. Notices Agilent Technologies Inc 2009 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G7100 90000 Edition 12 2009 Printed in Germany Agilent Technologies Deutschland GmbH Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished
27. Only use the fuses specified for this instrument The use of other fuses or materials is prohibited 182 Agilent 7100 Capillary Electrophoresis System User Manual Hardware Information 7 Installing Drainage Tubing The tubing drains condensation out of the instrument which may appear if you connect an external water bath to cool your samples in the sample tray On the left rear side of your instrument you may install silicone tubing Place a beaker of at least 200 mL capacity under this tubing to collect the drained condensation Agilent 7100 Capillary Electrophoresis System User Manual 183 7 Hardware Information External Water Bath for Tray Cooling Optional The two connections on the left rear of the instrument see Figure 2 on page 59 can be used to install an external water bath for the tray of the Agilent CE instrument The temperature for the cooling liquid is set at the external water bath The temperature display in the CE diagram displays the actual temperature of the tray controlled by the cooling liquid The external water bath cannot be controlled by the Agilent CE instrument Good Practices To avoid problems when using the external water bath consider the following points e The tubes used should have thermal insulation to avoid condensation Use a displacer to minimize the amount of liquid that is released in case of leaking cooling lines The device should be equipped with a liquid level sens
28. 137 5 138 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis Plus High Pressure CE p Outlet Lift Connection to the Tubing System The outlet lift is not connected to the tubing and valve system of the instrument by default Please connect the respective PTFE tube provided in your accessories kit The tube is labeled with an 8 and has one open end and one fitting To check whether this tubing is already installed you should load a vial in the outlet lifter from the CE diagram and locate the tube coming from the outlet lift Figure 36 shows an outlet lift with mounted tubing Outlet lift Tube 8 connected Figure 36 Outlet lift with mounted tubing for high pressure application Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 WARNING Damage or contamination of pressure valves Make sure tube 8 is connected properly before you apply high pressure to any lift High pressure may flush liquid into the pressure valves and lead to permanent damage Connect tube 8 prior to using high pressure Steps to Install the Outlet Lift Connection to the Tubing Please refer to the section Cleaning the Electrodes Pre punchers and Insulation Plate on page 196 for more details on individual steps of the following procedure Unload all lifter positions Switch off the instrument Disconnect the
29. MAC address and proceed with the respective initialization modes in Initialization Mode Selection on page 65 and the installation instruction in the Installing your ChemStation for LC and CE manual Ask your administrator to provide you with an IP address a subnet mask address and a gateway address In addition to LAN communication the instrument allows serial communication access via the RS 232 cable to the COM port of the PC However the data transfer rate would be too low for running the instrument at full performance This alternative data connection is only meaningful if the Lab Advisor software is for example used for troubleshooting reasons see Agilent Lab Advisor Software on page 169 Please see Starting Your Computer and the Agilent ChemStation Software on page 81 for information on configuration of the connection settings of the ChemStation software Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration 4 TCP IP Parameter Configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are P address Subnet mask Default gateway The TCP IP parameters can be configured by the following methods by automatically requesting the parameters from a network based BOOTP service using the so called bootstrap protocol see Agilent Bootp Service on page 75 by manually setting the
30. Setting Up a Test Sample Run on page 252 normal current values are below 50 uA If you set the lower alarm limit and the it is not exceeded 30 seconds after start of the run the run is stopped When the lower alarm limit is reached during the run part of the analysis when the HV is applied your current run is stopped During a sequence the system continues with the next analysis Do not use the lower alarm limit or program the time if changing the inlet or outlet vial during the run for example for fraction collection Otherwise the system will stop when changing the vial because the lower alarm limit is reached Agilent 7100 Capillary Electrophoresis System User Manual 99 5 100 Using the Agilent 7100 Capillary Electrophoresis System Posttime and Stoptime The stoptime defines the time for your separation also referred to as the run part of the analysis It does not include replenishment preconditioning injection and postconditioning After the stoptime has elapsed the system changes from the run state to postconditioning or postrun if defined and then to the ready state The posttime is normally used for equilibration It defines the time after the analysis that is after the stoptime has elapsed and data analysis is completed During the posttime the instrument is in the postrun state After both stoptime and posttime have elapsed the instrument completes postconditioning and is then ready for the next analysis R
31. a set of new foam bubbles is introduced into the vial before the fresh buffer The surface sensing system recognizes the first foam bubble as liquid So the final liquid level is not at the height it should be It may also happen that the vial contains no buffer liquid but only foam The result could be seen as low current or even no current supplied As a workaround we recommend programming a replenishment program in two steps 1 Replenish to a level about 0 2 cm higher than required 2 Fill to the desired level If the fill results a level higher than the desired one the vial is emptied to the level without using the sensing function and the foam has no influence As an example the desired height is 1 4 cm 1 Replenish vial XX to 1 6 cm 0 2 cm more 2 Fill vial XX to 1 4 cm Alternative No Replenishment Necessary 1 Fill several vials with buffer 2 Program methods which use these vials as the inhome and outhome vials for example vials 4 5 6 7 Method 1 inhome 4 outhome 5 Method 2 inhome 6 outhome 7 168 Agilent 7100 Capillary Electrophoresis System User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a stand alone product that can be used independently of a data system Agilent Lab Advisor software helps to manage the lab for high quality electrophoresis and chromatographic results and can monitor in real time a single Agilent CE or all the Ag
32. el proveedor de disolventes especialmente cuando se utilicen productos t xi cos o peligrosos Agilent 7100 Capillary Electrophoresis System User Manual 245 10 Appendix Simbolos de seguridad Tabla 31 Simbolos de seguridad S mbolo Descripci n PeO P El aparato se marca con este s mbolo cuando el usuario deber a consultar el manual de instrucciones como protecci n contra el riesgo de dafiar al operario y para proteger el aparato de dafios Indica voltajes peligrosos Indica un terminal conductor protegido Pueden producirse da os oculares al mirar directamente la luz producida por la l mpara de xen n que utiliza este equipo El aparato se marca con este s mbolo cuando el usuario est expuesto a superficies calientes que no deber tocar cuando est n a gran temperatura MONS ADVERTENCIA advierte de situaciones que podr an causar dafios personales o la muerte Nocontin e despu s de un aviso hasta que no lo haya entendido perfectamente y se cumplan las condiciones indicadas PRECAUCI N PRECAUCI N advierte de situaciones que podr an causar una p rdida de datos o da ar el equipo No contin e despu s de un mensaje de este tipo hasta que no lo haya comprendido perfectamente y se cumplan las condiciones indicadas 246 Agilent 7100 Capillary Electrophoresis System User Manual EE 10
33. gt 0 1 minutes 2 0 s response time 2 5 Hz Stoptime As with CE Posttime Off Spectrum None Autobalance Prerun Autobalance Lamps Required for Acquisition UV lamp Timetable Noentry Agilent 7100 Capillary Electrophoresis System User Manual 255 10 Appendix Chemical and Biological Safety Toxic and hazardous solvents The handling of solvents and reagents can hold health risks When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when using toxic or hazardous solvents Pathogenic toxic radioactive samples and genetically modified organisms If pathogenic toxic or radioactive samples are intended to be used in this instrument it is the responsibility of the user to ensure that all necessary safety regulations guidelines precautions and practices are adhered to accordingly This includes also the handling of genetically modified organisms Ask your laboratory safety officer to advise you about the level of containment required for your application and about proper decontamination or sterilization procedures to follow if fluids escape from containers Observe all cautionary information printed on the original solution containers prior to their use Because leaks spills or loss of sample may generate aerosols ob
34. me d lectrophor se capillaire Agilent 7100 doit tre plac sur une paillasse assurez vous que celle ci est con ue pour supporter le poids du syst me En fonctionnement l instrument doit tre en position verticale Environnement ATTENTION Votre instrument fonctionnera conform ment aux sp cifications dans les conditions de temp rature ambiante et d humidit relative pr cis es dans le Tableau 6 page 31 Condensation l int rieur du module La condensation endommage les circuits lectroniques du syst me e N entreposez pas ne transportez pas et n utilisez pas votre module dans des conditions o les fluctuations de temp rature risqueraient de provoquer de la condensation l int rieur du module Sile module a t transport par temps froid ne le sortez directement pas de son emballage laissez le atteindre progressivement la temp rature ambiante pour viter toute condensation 30 Agilent 7100 Capillary Electrophoresis System User Manual Caracteristiques physiques Site Requirements and Specifications 2 Tableau 6 Caract ristiques physiques Type Caract ristique Commentaires Poids 35 kg Dimensions 350 x 510 x 590 mm largeur x profondeur x hauteur Tension secteur Fr quence secteur Puissance consomm e Temp rature ambiante hors fonctionnement Temp rature ambiante hors fonctionnement Humidit Altitude de fonctionnement Refroidissement ext rieur Pression ext
35. tigt an jeder Seite zus tzlich 2 5 cm Platz und ungef hr 8 cm an der R ckseite f r die elektrischen Anschl sse und f r eine ausreichende Luftzirkulation Wenn das Agilent 7100 Kapillarelektrophorese System auf einem Labortisch aufgestellt werden soll stellen Sie sicher dass der Tisch ausreichende Tragf higkeit f r das System aufweist Das Ger t ist stehend zu betreiben Umgebungsbedingungen Ihr Ger t arbeitet bei der Umgebungstemperatur und der relativen Luftfeuchtigkeit die in den Spezifikationen in Tabelle 4 auf Seite 26 angegeben sind VORSICHT Kondensation im Inneren des Moduls Kondensation f hrt zur Besch digung der Systemelektronik Vermeiden Sie die Lagerung den Versand oder den Betrieb der Pumpe unter Bedingungen die zu einer Kondensation in der Pumpe f hren k nnen Nach einem Transport bei niedrigen Temperaturen muss das Ger t zur Vermeidung von Kondensation in der Verpackung verbleiben bis es sich auf Raumtemperatur erw rmt hat Agilent 7100 Capillary Electrophoresis System User Manual 25 2 Site Requirements and Specifications Abmessungen und Gewicht 26 Tabelle 4 Abmessungen und Gewicht Typ Spezifikationen Kommentare Gewicht 35 kg Abmessungen 350 x 510 x 590 mm Breite x Tiefe x H he 23 2 x 13 8 x 20 1 Zoll Netzspannung 100 240 V Wechselstrom 10 Weitbereichsnetzteil Netzfrequenz 50 oder 60 Hz 5 Leistungsaufnahme 350 VA 300 W 1024 BTU h Maximal zul s
36. Personensch den u U mit Todesfolge f hren k nnen Fahren Sie bei einer Kennzeichnung durch einen Sicherheitshinweis erst fort wenn Sie den Hinweis vollst ndig verstanden und entsprechende Ma nahmen getroffen haben VORSICHT Der Sicherheitshinweis ACHTUNG weist Sie auf Situationen hin die zu einem m glichen Datenverlust oder zu einer Besch digung des Ger ts f hren k nnen Fahren Sie bei einer Kennzeichnung durch diesen Sicherheitshinweis erst fort wenn Sie diesen vollst ndig verstanden und entsprechende Ma nahmen getroffen haben Agilent 7100 Capillary Electrophoresis System User Manual 237 10 Appendix Informations g n rales de s curit Les consignes g n rales de s curit suivantes doivent tre respect es lors de toutes les phases de fonctionnement entretien et r paration de cet instru ment Le non respect de ces consignes ou des mises en garde sp cifiques non c es ailleurs dans ce manuel est en violation des normes de s curit applicables la conception la fabrication et l usage pr vu de l instrument Agilent Technologies ne peut tre tenu responsable du non respect de ces exi gences par le client G n ralit s Cet instrument est un instrument de cat gorie I comportant une borne de mise la terre et a t fabriqu et contr l conform ment aux normes de s curit internationales Utilisation 238 Avant de brancher l alimentation lectrique effec
37. Stromversorgung Das Weitbereichsnetzteil des Ger ts arbeitet mit Standard Netzspannungen gem den Angaben in Tabelle 4 auf Seite 26 Daher ist an der R ckseite des Detektors kein Spannungswahlschalter vorhanden Auch im ausgeschalteten Zustand flie t im Modul Strom solange das Netzkabel eingesteckt ist Die Durchf hrung von Reparatur oder Wartungsarbeiten am Modul kann zu Personensch den wie z B durch Stromschlag f hren wenn das Modulgeh use ge ffnet wird w hrend das Modul an die Netzspannung angeschlossen ist Stellen Sie den freien Zugang zu den Netzkabeln sicher Trennen Sie das Netzkabel vom Ger t bevor Sie das Geh use ffnen Schlie en Sie das Netzkabel nicht an das Ger t an w hrend der Elektronikkasten an der Ger ter ckseite ge ffnet oder Seitenteile entfernt sind Falsche Netzspannung am Ger t Wenn das Ger t an eine h here Spannung als spezifiziert angeschlossen wird kann dies zu berspannungssch den oder einer v lligen Zerst rung des Ger ts f hren Schlie en Sie das Ger t an die angegebene Netzspannung an Agilent 7100 Capillary Electrophoresis System User Manual 23 2 Site Requirements and Specifications VORSICHT Unzug nglicher Netzstecker In einem Notfall muss es jederzeit m glich sein das Ger t vom Stromnetz zu trennen Stellen Sie sicher dass der Netzstecker des Ger ts leicht zug nglich ist Lassen Sie hinter der Netzbuchse des Ger ts gen gend Platz
38. Use recommended caps only see Table 23 on page 116 WARNING Instrument damage Avoid damage to the pre puncher electrodes and lifter Do not use crimp caps or any other metal caps when working with the Agilent CE instrument Make sure there are no air bubbles in the vials especially when using microvials for samples Current problems may occur or in the worst case the capillary may break heat generation or no conductance to the outlet buffer Ensure an adequate liquid level for sample buffer and waste vials See Figure 25 on page 117 Agilent 7100 Capillary Electrophoresis System User Manual 115 5 116 Using the Agilent 7100 Capillary Electrophoresis System Table 23 Recommended vials and caps for the Agilent CE instrument Item Description Part Number To Be Used For e Vial clear glass 2 mL Sample and buffer Pack of 100 5182 9697 Pack of 500 5183 4623 Vial amber glass 2 mL Pack of 100 5183 4619 Pack of 500 5183 4622 Vial polypropylene 1 mL 5182 0567 Sample and buffer Pack of 100 Vial polypropylene glass lined 9301 0977 Sample only 100 uL Pack of 100 Vial polypropylene 100 pL 9301 0978 Sample only Pack of 1000 y Snapcap polyethylene olefin Sample and buffer but not for septum for chemical resistance multiple use white color 5181 1507 Pack of 100 5181 1513 Pack of 500 PUR polyurethane septum 5181 1512 Sample and buffer Preferred standard caps clear color P
39. Using the Agilent 7100 Capillary Electrophoresis System Injection Options with CE p The installed high pressure supply may also be used for injection purposes using the Timetable option in the Injection field of a method setup From this menu you may select the High pressure flush command menu during injection with the same parameter choices that are available during Preconditioning This may be used to perform a pressure injection while using a capillary filled with replaceable gel Please note that the external pressure is not as precise as the injection pressure of up to 100 mbar Timetable Options with CE p The installed high pressure supply may also be used in the general timetable of a method setup The Pressure command is extended by the External pressure functionality see Figure 42 on page 146 Pressure Off Internal Flush 950 mbar internal Raw External Pressure External 80 bar Apply pressure to Inlet Apply pressure to Outlet Figure 42 Pressure options in CE p mode Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Timetable controlled changes in the pressure settings are OFF Pressure usage is switched off nternal pressure 100 mbar to 100 mbar Can be applied to the inlet vial Flush Standard flush procedure is applied with an internally generated pressure of approximately 950 mbar External Allows pressu
40. a drastic increase in the noise level is encountered which is not caused by any other reason for example contaminated slit something absorbing inside the capillary etc or the lamp refuses to ignite Generally the light intensity drops by 50 of its original value after approximately 2000 hours of operation We suggest that you check the lamp intensity before you change the lamp to be sure the noise increase is not due to other reasons air bubbles inside the capillary alignment interface or detection window contaminated Please use the Intensity Test in the Detector Test suite in the Lab Advisor Instrument Utilities software to determine the lamp intensity Check the Lab Advisor Instrument Utilities software to read out the lamp tag information on the current lamp lifetime It may happen that a hot lamp refuses to ignite the first time Please wait until the lamp has cooled down Only lamps provided by Agilent Technologies are supported in the 7100 CE instrument The lamp tag stores various data and details on the ignition program of the deuterium lamp A lamp without a tag will fail to ignite and could lead to instrument errors 224 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Removing the Existing Lamp Parts required Pozidriv screwdriver part of the accessory kit Deuterium lamp for the Agilent CE instrument 1 Select Exit from the File menu to exit the Agilent ChemStation Exit both online and o
41. ambient air through this filter to create pressure inside the electrolyte bottle The compressed air is needed to flush a capillary and to inject a sample Frequency Air filter replacement should be considered if the environment is very dusty or if the system cannot generate enough pressure We suggest that you change the air filter at least every three months Accessing the Air Filter The air filter is located inside the electrolyte and waste bottle compartment see Figure 71 on page 223 Therefore it is very easy to replace the air filter frequently If the instrument cannot build up either pressure or a vacuum check the following proper seal of the pressure bottle proper seal of the vacuum bottle and loose fittings on the bottles If none of these reasons apply call Agilent Technologies Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Changing the Air Inlet Filter Air inlet filter Figure 71 Air inlet filter NOTE Do not run the instrument without the air filter installed since particles may damage the valve system Agilent 7100 Capillary Electrophoresis System User Manual 223 8 Maintenance Changing the Lamp The following procedure is used to replace the deuterium lamp in the 7100 CE instrument Loss of light intensity over time increases baseline noise which may negatively influence the performance of your analysis You should consider exchanging the lamp if
42. and Diagnostics e CE Problem Solving 162 Agilent Lab Advisor Software 169 This chapter gives an overview of troubleshooting and diagnostic features and the different user interfaces RE Agilent Technologies 161 6 Troubleshooting and Diagnostics CE Problem Solving 162 The problem solving section in the instrument online help assists you in solving the most common failures Nevertheless a short list of problems are featured here Power On Fail The instrument will boot up after turning the instrument on by pressing the power on switch A green LED in the power switch is illuminated If the instrument power on fails the green control LED in the power switch may not light up Check the external power supply power cords and plugs for proper connection Check the line fuses next to the power plug on the right rear of the instrument e Check fuses 1 to 6 on the main electronic board A blown fuse is indicated by a small red LED visible through a hole in the metal shield of the instrument Agilent 7100 Capillary Electrophoresis System User Manual Troubleshooting and Diagnostics 6 E Problem Solvinc Leaks The leak sensor in the Agilent CE instrument is located in the lower right part of the instrument see Figure 50 Leak sensor Figure 50 Leak sensor position When the leak sensor is activated The error LED on the front of the instrument is illuminated The CE status displays an error Alog
43. and adjustments The tray as well as all three lift stations are moved The UV lamp will switch on during instrument startup based on parameters that can be defined in the DAD control section see Diode Array Detector on page 179 Otherwise the lamp can be switched on from the DAD diagram of the ChemStation The meaning of the LED colors on the front cover are explained in the following table Agilent 7100 Capillary Electrophoresis System User Manual 79 5 Using the Agilent 7100 Capillary Electrophoresis System Table 22 Status LED color Color No color Green Yellow Red Instrument is ready Analysis running do not use instrument with the exception of manual operation of the tray Some fields in DAD and CE diagram are shaded gray Not ready condition The run cannot be started yet When you start the LED will come on after a certain time Click the Agilent CE System status bar in the ChemStation to see what is not ready for example temperature Error condition There was or is currently a problem with the instrument or communication to the PC which requires your action or an acknowledgement To get more information connect to the instrument with the Lab Advisor or ChemStation software Check the logbook A blinking red LED indicates the instrument is in a resident mode This means only a core of the firmware is active and the instrument has limited functionality The Lab Advisor software will be a
44. and met the indicated conditions 234 Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Allgemeine Sicherheitsinformation Die folgenden allgemeinen Sicherheitshinweise sind in allen Betriebsphasen sowie bei der Wartung und Reparatur des Ger tes zu beachten Die Nichtbe achtung dieser Vorsichtsmassnahmen bzw der speziellen Warnungen inner halb dieses Handbuchs verletzt die Sicherheitsstandards der Entwicklung Herstellung und vorgesehenen Nutzung des Ger tes Agilent Technologies bernimmt bei Nichteinhaltung dieser Vorschrift durch den Kunden keine Haf tung Allgemein Dies ist ein Ger t der Sicherheitsklasse I mit Erdungsanschluss Es wurde entsprechend internationaler Sicherheitsstandards gefertigt und getestet Betrieb Beachten Sie vor dem Anlegen der Netzspannung die Installationsanwei sungen Dar ber hinaus sind folgende Punkte zu beachten W hrend des Betriebs darf das Geh use des Ger ts nicht ge ffnet werden Vor dem Einschalten des Ger tes m ssen s mtliche Massekontakte Verl nge rungskabel Spartransformatoren und angeschlossenen Ger te ber eine geer dete Netzsteckdose angeschlossen werden Bei einer Unterbrechung des Erdungsanschlusses besteht die Gefahr eines Stromschlags der zu ernsthaften Personensch den f hren kann Das Ger t muss au er Betrieb genommen und gegen jede Nutzung gesichert werden sofern der Verdacht besteht dass die Erdung besch digt ist Vergewisser
45. and open its window If required modify the parameters for the CE instrument according to your needs using the existing configuration Press OK to exit the Bootp manager Now turn on the CE instrument and view the Bootp server window After some time the Agilent Bootp Service will display the request from the LAN interface The parameters are now stored permanently in the non volatile memory of the CE instrument Close the Agilent Bootp Service and turn off the CE instrument Change the settings of the CE instrument s configuration switch to Using Stored mode see Table 18 on page 65 Power cycle the CE instrument The CE instrument can be accessed now via LAN without the Agilent Bootp Service refer to Agilent Bootp Service on page 75 Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration 4 Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the CE instrument It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters if the initialization mode selection switches permit this Non Volatile RAM Stored Parameter Figure 7 Manual configuration principle Agilent 7100 Capillary Electrophoresis System User Manual 71 4 72 LAN Configuration With Telnet Whenever a TCP IP connection
46. are recorded a spectra icon is visible in the detector diagram of the graphical user interface Detector Stoptime and Posttime You can define a special stoptime and posttime for your detector If you want to use the times set for the Agilent CE instrument set them to as CE Analog Output The Analog Output section enables you to set up the zero offset and attenuation of the analog output signal Indirect UV The section Indirect UV enables you to set a margin for peaks with negative absorbance Set it as close to the baseline as possible but allowing enough range for the negative peaks you expect to get The higher the value the lower the resolution of the detector and the greater the baseline noise Set this value only if you expect negative absorbances greater than 100 mAU Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Autobalance Enables you to zero the baseline of the diode array detector before or after an analysis You can use the autobalance feature to ensure that the baseline is reset to zero either before the Prerun time has elapsed or after the Postrun time has elapsed Lamps On Required for Acquisition Deselect the parameter Lamp on required for analysis if you also have a different detector configured than the built in DAD Such a detector could be an MSD mass sensitive detector LIF laser induced fluorescence or a CCD contactless conductiv
47. capillary zone electrophoresis CZE micellar electrokinetic chromatography MEKC and capillary isoelectric focussing CIEF The current CE mode for your instrument can be selected under Instrument lt more CE lt Instrument configuration The current mode is visible in the second line of the CE control diagram For detailed information see Working with the CE Diagram on page 84 and Graphical User Interface Explanation on page 86 Agilent CE Method Parameters This section describes the parameters of an Agilent CE method for data acquisition and their meanings Example sets of parameters for a method to analyze the test sample are in Setting Up a Test Sample Run on page 252 After you have completely set up the Agilent CE method and supplied the sample information you can run your method Choose Run Method from the Run control menu Overview A convenient way to access the parameters of an Agilent CE method is the item Setup Instrument Method in the Instrument menu This displays the CE Method Setup dialog box Tabs with parameters for the DAD and CE part of a method can be edited on this screen The left hand side shows the most important parameters The right hand side offers several pages that can be expanded subsequently for editing Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis CE Figure 20 CE Metho
48. capillary in the alignment interface use an ultrasonic bath to clean the interface and remove quartz residues Agilent 7100 Capillary Electrophoresis System User Manual 165 6 166 Troubleshooting and Diagnostics Broken Capillary To check for a broken capillary remove the capillary cassette and open it as described under Removing the Capillary Cassette from the Agilent CE Instrument on page 121 The capillary is especially sensitive to breaking at the detection window and the capillary ends therefore check these parts of the capillary If the capillary is broken at the detection window a leakage current of nearly the same value as the expected current may occur Small instabilities in the current curves as shown in Figure 51 may also be a sign of a broken capillary Figure 51 Current instabilities If the capillary is broken at the ends it may no longer reach into the liquid in the vial In that case the analysis is stopped if the low current alarm limit has been reached this must be set first Sometimes the capillary has very small cracks which are hardly recognizable under a microscope Exchange the broken capillary Contaminated Insulation Plate Salt crystals or other contamination of the insulation plate may cause leakage current e Clean the insulation plate as described in Cleaning the Insulation Plate on page 207 Agilent 7100 Capillary Electrophoresis System User Manual Troubleshooting and
49. caps Inspect for damage The chosen frequency may be time based for example weekly or injection based for example 100 injections It depends on your individual application and needs The time and injection based EMF counter can be customized 192 Do not open the top cover or remove the cartridge without lowering the lifts Use the function maintenance position from the tools section of the Lab Advisor Careless opening may lead to floating of electrodes with buffer or sample which leads to contamination or Agilent 7100 Capillary Electrophoresis System User Manual List of Standard Maintenance Procedures Clean the electrodes Clean the replenishment needle Clean the pre punchers Check alignment of electrodes Clean the replenishment tubing system Change the air inlet filter Change the buffer frit Change the UV lamp Clean the insulation plate Perform a full system diagnostic test Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 193 Early Maintenance Feedback EMF 194 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged or cleaned should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The Early Maintenance Feedback EMF feature monitors the usage of specific components in the instrument and pr
50. en elbanco 40 Entorno 40 Especificaciones f sicas 41 Especificaciones de rendimiento 42 43 43 44 45 45 46 47 48 48 49 50 50 51 52 Installing the Instrument 53 Unpacking the Agilent CE Instrument 54 Damaged Packaging 54 Unpacking the CE Instrument 54 Agilent 7100 Capillary Electrophoresis System User Manual Contents Delivery Checklist 55 Installing the Instrument 57 Physical Connection 57 4 LAN Configuration 61 What You Have to Do First 62 TCP IP Parameter Configuration 63 Configuration Switch 64 Initialization Mode Selection 65 Link Configuration Selection 69 Storing the Settings Permanently with Bootp 70 Manual Configuration 71 With Telnet 72 Agilent Bootp Service 75 Location of the Media Access Control MAC Address 76 5 Using the Agilent 7100 Capillary Electrophoresis System 77 System Definition 78 Controlling the Agilent CE Instrument 79 Before You Start the Agilent CE Instrument 79 Starting Up the Agilent CE Instrument 79 Starting Your Computer and the Agilent ChemStation Software 81 Starting the Agilent ChemStation Software the First Time 82 Working with the CE Diagram 84 Common Software Toolbar 84 Software Toolbar 85 Graphical User Interface Explan
51. flow rate of water is approximately 120 to 150 mL min Please note the individual viscosity of your buffer system It is important to make sure that there is enough buffer for example a minimum of 300 mL in the electrolyte bottle for the Flush Tubes operation An excessive Flush Tubes time will drain the electrolyte bottle causing air bubbles to be introduced into the system The Flush Tubes command does not include a cleaning of the replenishment needle It requires an execution of the Clean Tubes function to clean the last part to the fill needle of the replenishment needle Agilent 7100 Capillary Electrophoresis System User Manual 219 220 Maintenance Clean Tubes The Clean Tubes operation is meant to replace liquid in tubing from the electrolyte bottle to the replenishment needle and from the replenishment needle to the waste bottle A vial is lifted to the replenishment needle and filled several times The number of filling emptying cycles can be set by users using the Lab Advisor Instrument Utilities software This operation is meant for buffer change purposes or for cleaning with water It is important to make sure there is enough buffer in the electrolyte bottle for the Clean Tubes number of filling emptying cycles specified for example a minimum of 50 mL An excessive Clean Tubes time will drain the electrolyte bottle causing air bubbles to be introduced into the system The volume in the tubing system from electrolyte bo
52. fusibili Agilent 7100 Capillary Electrophoresis System User Manual 241 10 Appendix 242 Alcune modifiche descritte nel manuale devono essere effettuate con la cor rente collegata e lo strumento privo di coperchi La corrente presente in molti punti pu in caso di contatto provocare lesioni alle persone Qualsiasi operazione di modifica manutenzione e riparazione dello strumento aperto sotto tensione deve essere per quanto possibile evitata Queste opera zioni quando inevitabili devono essere eseguite da persone competenti e con sapevoli del rischio a cui sono sottoposte Non tentare riparazioni o modifiche interne se non presente un altra persona in grado di prestare soccorso e ria nimazione Non sostituire parti con il cavo di alimentazione collegato Non usare lo strumento in presenza di gas infiammabili o fumi L uso dello strumento al pari di altre apparecchiature elettriche in queste condizioni pu compromettere la sicurezza Non installare parti di ricambio e non effettuare modifiche non autorizzate I condensatori all interno dello strumento possono essere ancora carichi anche se lo strumento non collegato alla presa di corrente Questo strumento utilizza tensioni pericolose in grado di provocare gravi lesioni alle persone Usare collaudare e riparare lo strumento con la massima cautela Durante l uso di solventi osservare sempre le procedure di sicurezza idonee ad esempio indossare bracciali ed abiti a
53. if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Research Use Only Agilent 7100 Capillary Electrophoresis System In This Guide This guide contains information to install and operate the Agilent 7100 Capillary Electrophoresis System 1 Introduction This chapter gives an introduction to the instrument instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Instrument In this chapter you will find information about how to unpack and to set up the Agilent CE and information about the configuration of Instrument 4 LAN Configuration This chapter provides information on connecting the CE instrument to the Agilent ChemStation PC 5 Using the Agilent 7100 Capillary Electrophoresis System This chapter provides information on how to set up the CE instrument for an analysis and explains the basic settings 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 7 Hardware Information This chapter describes the instrument in more detail on hardware and electronics 8 Maintenance This chapter describes the maintenance of the CE in
54. l utilisateur de consulter le manuel d utilisation afin de prot ger l op rateur contre tout danger et d viter d endommager l appareil Indique des tensions dangereuses Indique une borne de mise la terre Indique qu il est dangereux pour les yeux de regarder directement la lumi re produite par la lampe au deut rium utilis e dans ce produit L appareil comporte ce symbole pour indiquer qu il pr sente des surfaces chaudes et que l utilisateur ne doit pas les toucher lorsqu elles sont chaudes AVERTISSEMENT PeO b UN AVERTISSEMENT vous met en garde contre des situations qui pourraient causer des blessures corporelles ou entrainer la mort e N allez pas au del d une mise en garde Avertissement tant que vous n avez pas parfaitement compris et rempli les conditions indiqu es ATTENTION Le message ATTENTION vous pr vient lors de situations risquant d entra ner la perte de donn es ou d endommager l quipement e N allez pas au del d une mise en garde Attention tant que vous n avez pas parfaitement compris et rempli les conditions d finies 240 Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Informazioni generali sulla sicurezza Le seguenti precauzioni generali di sicurezza devono essere rispettate durante tutte le fasi di utilizzo manutenzione e riparazione dello strumento Il man cato rispetto di tali precauzioni o di avvertenze specifiche riportate in altri punti
55. of approximately 600 V cm Injection Because of the resistance of the packed bed the usual applied pressure of up to 50 mbar is insufficient to introduce sample onto the capillary In this case high pressure and or electrokinetic loading may be used although the limitations of this technique should be noted Problem Solving Offline purging If air bubbles occur during an analysis the current will become instable or very low lt 0 3 uA If this occurs remove the packed capillary and connect it to an LC pump capable of delivering a flow rate of 40 uL min to the capillary Great care should be taken when removing and handling the capillary and when connecting and disconnecting the capillary to or from the pump Fused silica packed capillaries are extremely fragile Instrument If the capillary is filled with solvent then this may be replaced by inserting the purging capillary into the instrument and flushing with external pressure using the external gas supply icon on the CE diagram Generally 10 60 minutes are sufficient to replace the storage solvent with the operating mobile phase depending on the velocity of EOF Agilent 7100 Capillary Electrophoresis System User Manual 159 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography CEC 160 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System M User Manual 00 ee 6 69 Troubleshooting
56. only be done in the resident system Updates to the resident system can only be done in the main system Main and resident firmware must have the same version Agilent 7100 Capillary Electrophoresis System User Manual 177 7 Hardware Information Main firmware update Resident system Main system Resident firmware update Figure 54 Firmware update mechanism The latest firmware revisions and documentation are available on the Agilent website http www chem agilent com 178 Agilent 7100 Capillary Electrophoresis System User Manual Hardware Information 7 Diode Array Detector You can control external devices as part of the method through the relay contact connectors on the rear of your instrument The DAD is directly controlled by the DAD diagram through method parameters and general control settings Direct control of the DAD and control through method parameter is described in Controlling the Agilent CE instrument Detector Parameters on page 107 and the section User interface in the ChemStation online help Agilent 7100 Capillary Electrophoresis System User Manual 179 7 Hardware Information DAD Control In the Method and Run Control of the ChemStation select Instrument gt More DAD Control gt Control to open the DAD control dialog Figure 55 on page 180 Control At Power On Y Turn UV lamp on Turn on at Figure 55 DAD control dialog You may switch the UV lamp on or off
57. parameters using Telnet The LAN interface differentiates between several initialization modes The initialization mode abbreviation init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 17 on page 64 Agilent 7100 Capillary Electrophoresis System User Manual 63 4 LAN Configuration Configuration Switch The configuration switch can be accessed on the right rear side of the instrument The 7100 CE is shipped with switches 7 and 8 set to ON This means that the instrument is set to a fixed default IP 192 168 254 11 To perform any LAN configuration SW1 and SW2 must be set to OFF Table 17 Factory default settings Initialization init mode Using default switches 7 and 8 set to ON Link configuration Speed and duplex mode determined by auto negotiation 64 Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration 4 Initialization Mode Selection The following initialization init modes are selectable Table 18 Initialization mode switches SW6 SW7 SW8 Init mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default Bootp When the initialization mode Bootp is selected the 7100 CE tries to download the parameters from a Bootp s
58. per elettroforesi capillare Agilent 7100 assicurarsi che il banco sia in grado di sostenere il peso complessivo del sistema Lo strumento deve essere usato in posizione verticale Ambiente AVVERTENZA Lo strumento progettato per essere usato nelle condizioni di temperatura ambientale e umidit specifiche descritte nella Tavola 8 a pagina 36 Condensa all interno del modulo La condensa danneggia i componenti elettronici del sistema Non immagazzinare trasportare o utilizzare il modulo in condizioni in cui eventuali variazioni di temperatura potrebbero causare la formazione di condensa al suo interno Seil modulo stato spedito in condizioni di bassa temperatura lasciarlo nel contenitore di imballaggio per consentirgli di raggiungere lentamente la temperatura ambiente ed evitare la formazione di condensa Agilent 7100 Capillary Electrophoresis System User Manual 35 2 Site Requirements and Specifications Specifiche fisiche 36 Tavola 8 Specifiche fisiche Tipo Specifica Commenti Peso 35 kg Dimensioni 350 x 510 x 590 mm larghezza x profondit x altezza Tensione di rete Frequenza di rete Consumo elettrico Temperatura ambiente non operativa Temperatura ambiente non operativa Umidit Altitudine operativa Raffreddamento esterno Pressione esterna Standard di sicurezza IEC CSA UL Involucri 100 240 VCA 10 50 o 60 Hz 5 350 VA 300 W 1024 BTU h 5 40 C
59. pins and are parallel to them Use the capillary lock to make adjustments to the inlet side of the capillary The capillary end on the outlet side is fixed it cannot be moved Agilent 7100 Capillary Electrophoresis System User Manual 131 5 Using the Agilent 7100 Capillary Electrophoresis System Inserting the Cassette Use the Change Cassette task from the CE diagram to access an already installed cassette To insert the capillary cassette into the system you have to perform the following steps 1 Choose the cassette icon in the CE diagram and select Change Cassette from the menu This removes the buffer vials from the ends of the capillaries and back to their position in the tray This will take approximately 5 seconds 2 Open the top cover to access the cassette 3 Press a spring located in the middle of the front lid to release the latch Figure 34 shows the position of an installed cassette 132 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 4 Move the cassette guide to the front Extracting the cassette from the instrument without lowering the lifts will lead to a higher contamination of the inner parts of the electrode or pre puncher Crystallization or liquid residue of this contaminations might bias future runs Cassette Cassette guide Figure 34 Insertion of cassette CAUTION Capillary damage by incorrect insertion into capilla
60. power cord can lead to an electric shock or short circuit Never operate your instrument from a power outlet without a ground connection Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 7100 Capillary Electrophoresis System User Manual 19 2 Site Requirements and Specifications Bench Space The instrument dimensions and weight mean that the instrument can be placed on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the Agilent 7100 Capillary Electrophoresis instrument is to be placed on a bench make sure that the bench is designed to bear the weight of the system The instrument must be operated in a upright position Environment CAUTION Your instrument will work within the specifications for ambient temperatures and relative humidity described in Table 2 on page 21 Condensation within the module Condensation will damage the system electronics Do not store ship or use your module under conditions where tempera
61. pre puncher again 204 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Cleaning the Electrodes 1 Flush the electrodes with water Watch for salt crystals All salt crystals must be removed Figure 64 Flushing electrodes 2 Flush the electrodes with isopropanol 3 Use compressed air to dry the electrodes and remove all traces of liquid CAUTION Increased leakage current and arcing due to wet electrodes Increased leakage current and arcing might appear during analysis if electrodes are not completely dry To avoid contamination the inside of the electrodes must be completely dry before reinstallation If there is still an accumulation of dirt in the upper funnel or inside the electrode start the cleaning procedure again You may place it in a beaker of water or isopropanol and treat this in an ultrasonic bath for 5 minutes followed by steps 2 and 3 in this section If this fails replace the electrode Agilent 7100 Capillary Electrophoresis System User Manual 205 8 Maintenance Cleaning the Pre punchers Check for damaged or bent tips before cleaning the pre punchers If the tips are damaged replace the pre punchers Check for grooves in the funnels of the pre punchers Grooves are a sign of improper alignment of the electrodes and may lead to improper sealing Improper sealing may lead to problems during flushing injection or CEC applications Use the alignment center of the Lab Advi
62. range see Physical Specifications on page 21 The lowest applicable temperature is 10 C below ambient temperature The highest temperature is 60 C The instrument is ready after the cassette temperature reaches the setpoint and stabilizes A run can be started The home values are activated at five points in your analysis When a method is loaded When you activate Run Method or Run Sequence from the RunControl menu After injection is completed After the stoptime has elapsed After the entire analysis including postconditioning is completed Keep the cassette temperature above the dew point or decrease the ambient humidity The instrument is specified to be operated at non condensing conditions which is at humidity below 80 at 31 C 87 8 F Please be aware if the temperature of the cassette is below the dew point condensation might take place Subsequently water condensate from the ambient might lead to arcing and damage of the cassette or instrument Agilent 7100 Capillary Electrophoresis System User Manual 97 5 Using the Agilent 7100 Capillary Electrophoresis System High Voltage System The following parameters for the power supply can be controlled using the CE Method Setup screen in the Agilent ChemStation software Enable High Voltage Voltage Current Power In addition you can set a Lower Alarm Limit for Current In order to directly apply HV from the dialog associated wi
63. rieure Normes de s curit CEI CSA UL Bo tier 100 240 V CA 10 50 ou 60 Hz 5 350 VA 300 W 1024 BTU h 5 40 C 40 70 C Inf rieure 80 31 C Jusqu 2 000 m Max 0 5 bar max 50 C 2 12 bar Cat gorie d installation Il degr de pollution 2 Tous les mat riaux sont recyclables Plage de tensions tendue Maximum Sans condensation Bain marie Air ou azote sans hydrocarbures Utilisation int rieure uniquement Pour les applications fonctionnant en mode ECC lectrochromatographie capillaire ou en mode EC p mode d lectrophor se capillaire avec utilisation optionnelle de pression sup rieure une alimentation en pression ext rieure d air ou d azote sans hydrocarbures une pression maximale de 15 bar peut tre utilis e Un adaptateur m le respectif adapt l adaptateur femelle de l instrument et des tuyaux PTFE sont livr s avec l instrument Le syst me d electrophorese capillaire Agilent 7100 est concu pour fonctionner dans un environnement lectromagn tique usuel dans lequel des metteurs de radiofr quences tels que les t l phones mobiles ne doivent pas tre utilis s proximit Agilent 7100 Capillary Electrophoresis System User Manual 31 2 Site Requirements and Specifications Caract ristiques de performance Tableau 7 Caract ristiques de performance du syst me d electrophorese capillaire Agilent 7100 Type Caract rist
64. stations to remove the capillary cassette Releasing the pressure to change the replenishment bottles Working with these items does not change the method currently loaded Set Error Method Set Error Method enables you to store the current parameters These parameters are applied to the instrument when an error occurs It ensures that the instrument shuts down in a controlled manner if ChemStation control is discontinued for any reason Identify Device The command Identify Device causes the LED on the front cover to blink alternating in green and yellow for 4 seconds Agilent 7100 Capillary Electrophoresis System User Manual 89 5 Using the Agilent 7100 Capillary Electrophoresis System System Vialtable The vialtable is a graphical tool which facilitates the management of vials in the tray It can be used as an intelligent notepad which exchanges its information with methods and sequences Some features are e Ifa vial is defined in the vialtable this definition name of vial will be used whenever the vial is referenced method editing method print Consistency checks can be done in order to verify if there are vial conflicts between the method and sequence usage of vials Documentation of vial positions and contents Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 How to Prepare your Agilent CE Instrument for Analysis To prepare the Ag
65. the alignment interface from the installation tool to secure the capillary in the alignment interface The detection window or the extended light path of the capillary must be visible through the detection interface If aligned correctly you can see the slit of the interface behind the detection window See Figure 30 on page 127 126 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis CE Nd Figure 30 Extended light path aligned correctly mi Figure 31 Extended light path aligned incorrectly Agilent 7100 Capillary Electrophoresis System User Manual 127 5 Using the Agilent 7100 Capillary Electrophoresis System Installing a Capillary in an Empty Capillary Cassette This procedure describes how to install a capillary in an empty capillary cassette If the cassette has already been installed in the Agilent CE instrument remove it as described above What You Will Do 1 Open the capillary cassette 2 Install the capillary in the empty cassette 3 Close the capillary cassette WARNING Risk of injury of sharp capillary ends Sharp capillary ends can injure your eyes or skin Wear safety glasses when opening the cassette and handling the capillary to protect your eyes and body Open the Capillary Cassette 1 Place the cassette flat on a solid surfa
66. the box and position it in a suitable location 54 Agilent 7100 Capillary Electrophoresis System User Manual Shipment container 7100 instrument Accessory boxes Installing the Instrument 3 Unpacking the Agilent CE Instrument Figure 1 Unpacking the Agilent CE instrument Delivery Checklist Ensure all parts and materials have been delivered with the instrument Please report missing or damaged parts to your local Agilent Technologies sales and service office Accessories The accessories shipped with the Agilent CE instrument are in additional boxes on right and left in the instrument box Check that you have received everything using Table 16 Agilent 7100 Capillary Electrophoresis System User Manual 55 3 Installing the Instrument Table 16 Accessories Description Part number Quantity HPCE startup capillary kit G7100 68700 1 Detector interface green 50 um G7100 60210 1 Detector interface red 50 um BF3 G7100 60230 1 Accessory kit G7100 68705 1 Coupler 0100 1633 2 0 ring 25 12 mm id 1 78 mm XSECT DIA 0905 1163 1 Container 1540 0307 1 Fuse T6 3AH 250 VAC 2110 1018 2 Fuse T6 3AL 250 VAC 2110 0623 2 Fuse T3 15AL 250 VAC 2110 1417 4 Air filter 3150 0619 1 Tubing assembly 5063 6527 1 Screwdriver slot shaft 8710 2607 1 Screwdriver Pozidriv shaft 8710 0899 1 12 mm socket wrench 8710 2076 1 Lift tubing assy G1600 67003 1 Vial crimp snap polypropylene 1 mL 1
67. under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclo sure of Software is subject to Agilent Tech nologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that
68. zum Herausziehen des Kabels Netzkabel Das Ger t wird mit unterschiedlichen Netzkabeln ausgeliefert Der weibliche Stecker ist bei jedem Netzkabel identisch Er wird an die Netzanschlussbuchse an der R ckseite angeschlossen Die Stecker am anderen Ende der Netzkabel sind unterschiedlich und erf llen die Normen unterschiedlicher L nder oder Regionen WARNUNG Nicht vorhandene Erdung oder Verwendung eines nicht spezifizierten Netzkabels Bei der Verwendung des Ger ts ohne Erdung oder mit einem nicht spezifizierten Netzkabel k nnen Stromschl ge und Kurzschl sse auftreten e Betreiben Sie Ihr Ger t niemals an einer Spannungsquelle ohne Erdung Verwenden Sie niemals ein anderes als das von Agilent zum Einsatz im jeweiligen Land bereitgestellte Kabel WARNUNG Verwendung nicht im Lieferumfang enthaltener Kabel Die Verwendung von Kabeln die nicht von Agilent Technologies geliefert wurden kann zu einer Besch digung der elektronischen Komponenten oder zu Personensch den f hren Verwenden Sie ausschlie lich Originalkabel von Agilent Technologies um eine einwandfreie Funktion und die Einhaltung der Sicherheits und EML Bestimmungen zu gew hrleisten 24 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Platzbedarf Die Abmessungen und das Gewicht des Ger ts erm glichen seine Aufstellung auf praktisch jedem Schreibtisch oder Laborarbeitstisch Das Ger t ben
69. 0 instrument 78 87 starting 79 instrument control 79 insulation plate 207 L Lab Advisor 169 lamp switching on 110 leaks 163 levelsensor 220 line frequency 21 line fuse holder 173 line power 58 line voltage 21 liquid level 115 buffer vials 118 outlet vials 119 sample vials 117 waste vials 119 lower alarm limit 99 M main system 176 maintenance 189 alignment interface 124 method parameters 92 253 N noise level 107 259 P peak width 107 264 polarity switching 98 posttime 100 power 99 power supply 21 pre puncher 206 cleaning 206 installation 203 prepuncher installation 203 pressure 103 raw data 176 reference wavelength 107 255 removing 225 removing cassette 121 replenishment system 100 installation of bottles 111 preparation 111 resident system 176 S Sample vials 117 liquid level 117 preparation 117 SDS 168 setup CE method 92 signals 107 spectra 108 starting instrument 81 91 stoptime 100 straight capillaries 124 switching during run 109 T Telnet 72 temperature 97 environment 97 test instrument 169 timetable 105 tray 87 Agilent 7100 Capillary Electrophoresis System User Manual tray cooling 184 manual operation 120 tubes clean 220 U unpacking 54 updates 177 V vial sensor 120 vials 115 voltage 21 98 W waste bottle 114 waste vials 119 water bath 184 wavelength 107 wavelength calibration 134 www agilent com In This Book This man
70. 0 Agilent Lab Advisor software comes with a complete set of features The Agilent Instrument Utilities software provides set of basic features These software packages assist you by providing hardware test suits a set of tools used during procedures Early Maintenance Feedback EMF parameters and scheduling functions You can find a detailed description in the respective online help documentation Agilent 7100 Capillary Electrophoresis System User Manual Overview of Maintenance Table 25 Overview of maintenance Maintenance 8 Procedure Typical frequency Notes Cleaning the electrodes Clean frequently Inspect pre punchers for salt deposits bent pre punchers and insulation tips deposits in top funnel plate Replace if either electrodes or pre punchers are bent show signs of wear such as scratches or cannot be cleaned Cleaning the electrodes Clean frequently Inspect for salt deposits O ring integrity bending Cleaning the detection window and the alignment interface Preparing cleaning the System cleaning should be performed replenishment system when new buffer is added to the electrolyte reservoir Maintaining the functionality Changing the air inlet filter Changing the lamp Perform lamp intensity test from the detector test suite in the Lab Advisor regularly for example bi weekly and monitor lamp life using EMF values and check alignment Capillary window Gently clean with isopropanol and a l
71. 0 Capillary Electrophoresis System Vials You can program a method to use certain vials as the inlet or outlet home vial During electrophoresis the electrodes and the capillary are in these vials Vial numbers 1 to 48 or user vials User 1 to User 10 can be selected The selection can be done from the pull down menu or by typing in the number with the keyboard Clicking Add Method Vial allows you to enter vial names which will be visible in the vialtable EE Add Method Vial Vial 4 successfully added to vials Figure 22 Add Method Vial User vials are numbered from 1 to 10 User vials are placeholders in the method which are resolved by the sequence table at a later point in time Usage of user vials is helpful for method optimization Various buffer compositions can be tested with one common method Creation of methods with variations of Inlet or outlet home vials can be omitted Usage of user vials is also helpful to change buffers in long sequences to avoid over utilization of the buffer Vial numbers below 10 must be typed in with a preceding 0 for example 02 for a vial in tray location number 2 since the vials are numbered with two digits Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Cassette Temperature The cassette temperature can either be set to not be controlled or to a certain setpoint This setpoint must be within the instrument s specified
72. 00 PK 5182 0567 1 Snap caps polyurethane 100 PK 5181 1512 1 Cable remote 5061 3378 1 CE catalog 5988 6616EN 1 LAN cable crossover 5023 0203 1 Bottle 500 mL electrolyte 9300 1748 2 HPCE Primer G7100 90001 1 CE System User s Guide G7100 90000 1 Glass filter solvent inlet 5041 2168 1 Electrode alignment assy G7100 60107 1 56 Agilent 7100 Capillary Electrophoresis System User Manual Installing the Instrument 3 Installing the Instrument Physical Connection Module is partially energized when switched off as long as the power cord is plugged in Repair or maintenance work on the module can lead to personal injuries for example a shock hazard when the cover is opened and the module is connected to the power Ensure that line power cord is disconnected before setting up your Agilent CE instrument In general you have to go through the following steps 1 Unpack the Agilent CE instrument and position it in a suitable location on your bench see Unpacking the Agilent CE Instrument on page 54 2 Connect the cables at the rear of the Agilent CE instrument 3 Install the bottles in the replenishment system see Preparing the Replenishment System if Needed on page 111 Agilent 7100 Capillary Electrophoresis System User Manual 57 3 58 Installing the Instrument Connecting Cables The Agilent CE instrument has the following connectors on the rear Allelectrical connectors such as line power LAN c
73. 100 Capillary Electrophoresis System User Manual Troubleshooting and Diagnostics 6 Blocked Replenishment Needle If you are using the replenishment function of the Agilent CE instrument buffer may precipitate inside the replenishment needle To avoid this put a vial filled with water or buffer to a height of 1 8 cm in vial position 49 Vial position 49 is the home position of the replenishment lift The vial will be picked up by the lift and brought to the replenishment needle The needle will dip into the water thus preventing the buffer from precipitating see Preparing Vials for Buffer and Sample on page 115 Vial position 49 Leakage Current Leakage current is indicated by a yellow bar in the current part of the energy status display in the CE diagram Only high leakage current will lead to this An instrument curve allows you to monitor current leakage even at low values from the ChemStation Leakage current means that the current at the inlet electrode is different from the one at the outlet electrode and thus a current flows outside the capillary A certain leakage current must be regarded as acceptable since it is not avoidable due to technical reasons It can be caused by A broken capillary An empty inject vial e An air bubble in injection vial or run buffer vials An air bubble in capillary e A contaminated insulation plate A high humidity level A conductive capillary surface If you find a broken
74. 199 Accessing the Liquid Handling Module 201 Accessing and Removing the Pre punchers 203 Cleaning the Electrodes 205 Cleaning the Pre punchers 206 Cleaning the Insulation Plate 207 Reinstalling the Pre punchers 208 Reinstalling the Electrodes and the Insulation Plate 210 Reinstall the Capillary Cassette 215 Reinstall the Front Cover 215 Maintenance of the Replenishment System 216 Changing the Buffer Composition in the Replenishment System 217 The Replenishment System Will Not Be Used for Some Time 218 Further Action 219 Operations for Replenishment Maintenance 219 Changing the Air Inlet Filter 222 Accessing the Air Filter 222 Changing the Lamp 224 Removing the Existing Lamp 225 Inserting a New Lamp 227 Further Action 227 Cleaning the Instrument 228 Agilent 7100 Capillary Electrophoresis System User Manual Contents 9 Parts and Materials for Maintenance 229 Overview of Maintenance Parts 230 10 Appendix 231 General Safety Information 232 Operation 232 Allgemeine Sicherheitsinformation 235 Informations g n rales de s curit 238 Informazioni generali sulla sicurezza 241 Informaci n de seguridad 244 247 250 Setting Up a Test Sample Run 252 Method Parameter CE Default Settings 253 Method Parameter DAD 255 Chemical and Biological Safety 256 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 257 Radio Interference 258 Sound Em
75. 7 Configuration 7 CE Part CE Part G7150A PP00000007 Y DAD Part F DAD Part 1671514 PP00000007 amp BinPump lsoPump e QuatPump Figure 15 Configuration menu Please refer to LAN Configuration on page 61 for the default IP address or manual configuration of an individual IP address After you have double clicked the Instrument Online icon the software is loaded with the last method used The software boots in the exact last status Do not use the instrument or the graphical user interface during initialization Wait until the system has reached the ready state If there is a vial in tray position 50 reserved position the system asks you to remove that vial before you proceed Duplicate vials are put in the tray and the respective position is brought to the front Duplicate vials may occur if vials from the tray are loaded in a lifter and later on another vial is loaded in the same tray position If there are no bottles electrolyte or waste in the replenishment system or they are not properly tightened the error LED red will come on because no pressure can be built up Check the logbook for more information Agilent 7100 Capillary Electrophoresis System User Manual 83 5 Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram When you start the Agilent ChemStation the CE and DAD diagram appears by default as the main screen The CE diagram displays th
76. 8 capillary cassette opening 128 removing 121 temperature 97 capillary conditioning 101 capillary detection window 125 capillary electrochromatography 148 capillary electrophoresis test kit 252 capillary insertion tool 125 capillary lock 129 carousel 130 cassette 129 closing 131 installation 132 installation of capillary 129 removing from instrument 121 temperature 97 cassette lock 129 CE diagram 84 CE timetable 105 change cassette function 121 changing 226 changing of fuses 182 checkout run 252 ChemStation 79 starting 81 cleaning 191 196 205 condensing water 20 97 conditioning 101 corresponding alignment interface detection window 125 extended light path 124 installation into alignment interface 125 corresponding alignment interfaces 124 corrosion 112 current 98 105 Agilent 7100 Capillary Electrophoresis System User Manual D detector 107 diagram 86 icon 86 lamp 86 230 parameters 94 107 posttime 108 signals 109 stoptime 108 timetable 109 detector cover 172 diagnostic 169 E electric parameters 98 electrodes 98 205 electrolyte bottle 87 environment 18 20 condition 20 extended light path capillaries 125 F firmware 176 177 flush tubes 219 fraction collection 135 front cover 198 G gradients 105 High Pressure home vials 115 humidity 97 263 Index high Pressure home vials 96 high pressure 136 148 humidity 20 inlet home vial 115 installation 21
77. Available Connectors Power connector Fuse netfilter 2 fuses 250 VAC T6 3AH part number 2110 1018 LAN connector 7100 to controlling PC CAN connectors 7100 to additional Agilent 1200 Series Module USB connector for future use RS232 connector serial interface to a computer This connection has a special out pin and is not compatible for connecting printers or plotters It is called null modem cable with full handshaking and the wiring is established between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 and 9 9 APG remote connector Agilent Technologies Analytical Products Group remote connector to be connected to Agilent modules for example to MS system Analog out BNC connector 0 to 0 1 V or 0 to 1 V Analog in BNC connector 0 to 1 V shield is analog and center is analog 8 bit configuration switch see Chapter 4 LAN Configuration starting on page 61 Fuses Fuse 1 Peltier T6 3AL 250 VAC part number 2110 0623 Fuse 2 HV supply CE detector board T3 15 AL 250 VAC part number 2110 1417 Fuse 3 CE sampler board with valve and lifter control T3 15 AL 250 VAC part number 2110 1417 Fuse 4 heater optional T3 15 AL 250 VAC part number 2110 1417 Fuse 5 processor 5 V 15 V 15 V supplies on mainboard air pump fans CE temperature sensor board and cassette fan T3 15 AL 250 VAC part number 2110 1417 Fuse 6 DAD T3 15 AL 250 VAC part number 2110 1417 Agilent 7100
78. Before Filling the Replenishment System The replenishment system bottles and tubing should be cleaned when Using it for the first time Changing the replenishment buffer Ifthe replenishment system will be idle for some time Filling the Replenishment System To prepare the replenishment system 1 Clickone of the replenishment bottles in the CE diagram 2 Choose Change Bottles to release the pressure before you open the bottles 3 Open the front door at the bottom of the front panel Hazardous organic solvents and sample residues The waste may contain organic solvents and residue from your sample Wear rubber gloves and safety glasses when handling electrolytes and deposit the waste in a safe waste container 112 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis CE 4 Take out the electrolyte bottle and unscrew the cap The electrolyte bottle has a frit and tubing inside Avoid contaminating the frit see Figure 24 on page 113 Filling the Replenishment Bottles Cap of electrolyte bottle Waste bottle Frit Electrolyte bottle Figure 24 Filling the replenishment bottles Agilent 7100 Capillary Electrophoresis System User Manual 113 5 114 Using the Agilent 7100 Capillary Electrophoresis System 1 Make sure the buffer is filtered use a 0 25 um filter For buffers w
79. CSA UL grado de contaminaci n 2 edificios Carcasa Todos los materiales son reciclables Para aplicaciones en modo CEC cromatografia electrocapilar o CE p electroforesis capilar con uso optativo de alta presi n puede emplearse una fuente externa de presi n a base de aire o nitr geno sin aceite a una presi n m xima de 15 bares A la entrega se suministra tambi n el correspondiente adaptador macho que encaja con el hembra y los tubos de PTFE del instrumento El sistema de electroforesis capilar Agilent 7100 est dise ado para funcionar en un entorno electromagn tico normal en cuya proximidad no deben usarse transmisores de RF como por ejemplo tel fonos m viles Agilent 7100 Capillary Electrophoresis System User Manual 41 2 Site Requirements and Specifications Especificaciones de rendimiento Tabla 11 Especificaciones de rendimiento del sistema de electroforesis capilar Agilent 7100 Tipo Especificaciones Comentarios Caracter sticas de seguridad Diagn sticos completos detecci n y presentaci n de errores detecci n de fugas l mite inferior de corriente Sensor de fuga de l quidos sensores de seguridad en la puerta y la tapa para desactivar el alto voltaje sensor de viales Control y evaluaci n de datos Agilent ChemStation en Windows XP SP3 Comunicaciones LAN red de rea de controlador CAN RS 232C De0a1V mando a distancia APG se ales de ready start stop y shut down USB
80. Capillary Electrophoresis System User Manual Hardware Information 7 Next to each fuse F1 to F6 is a LED on the rear of the electronic board This is visible through a cutout in the metal shield of the electronic box A red LED indicates that the respective fuse is blown If one of the fuses is blown the green LED of the power switch flashes WAFNING 7 Using wrong fuses may result in afire hazard For continued protection against fire hazards only replace fuses with the same type and rating Only use the fuses specified for this instrument Use of other fuses or materials is prohibited Agilent 7100 Capillary Electrophoresis System User Manual 175 7 Hardware Information Firmware The firmware for the instrument consists of two independent sections A non instrument specific section called the resident system An instrument specific section called the main system Resident System The resident section of the firmware is identical in many Agilent devices and modules Its properties include The complete communication capabilities CAN USB LAN and RS 232C e Memory management Ability to update the firmware of the main system Main System Its properties include The complete communication capabilities CAN USB LAN and RS 232C e Memory management Ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into
81. DAD status 10 Replenishment station 17 Lamp 11 Electrolyte bottle pressure storage 18 Detector wavelengths spectra 12 Waste bottle vacuum storage 19 Instrument identifier al 13 Current electrical values 20 Overall instrument status 14 Electrical parameter from method 21 Instrument mode Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Working with the CE Diagram The current analysis mode is displayed in the top line of the CE diagram The analysis modes that can be selected under instrument configuration are CE CE p CEC or CE MS For configuration without a DAD detector for example with external detectors the DAD diagram will not be displayed For configuration with an additional pump for example CE MS an additional pump diagram will be displayed Lid latch Cassette Detector Outlet lift Inlet lift Sample tray Replenishment lift Replenishment bottles Figure 18 Corresponding hardware components Agilent 7100 Capillary Electrophoresis System User Manual 87 5 Using the Agilent 7100 Capillary Electrophoresis System Menus in the CE and DAD Diagram The CE and DAD diagram offers access to directly control several instrument components as well as access to method parameters Right clicking in the background of each diagram provides access to the context menus Figure 19 DAD e Control Method Set Error Method CE PS
82. Diagnostics 6 Empty Injection Vial Access the vial position specified in the Sample info or the Sequence table using the Get vial function in the Tray control screen e Checkif the vial is filled Dirt in the Vials Clean all vials prior to use First flush them with water followed by for example some isopropanol Use compressed air to dry the vials inside Air Bubble in Injection Vial or Run Buffer Vials When filling sample or buffers into the vial an air bubble is sometimes formed This happens very frequently when using microvials Air bubbles can cause air to be injected instead of the sample To avoid air bubbles carefully fill vials starting from the bottom If you cannot avoid the formation of air bubbles adjust the lift offset in the Home values dialog box so that the capillary doesn t reach into the bubbles Air Bubble in Capillary Small air bubbles in the capillary may cause spikes in your electropherogram Larger air bubbles expand so much during analysis that the current drops below the lower alarm limit Flush your capillary with buffer to remove air bubbles Agilent 7100 Capillary Electrophoresis System User Manual 167 6 Troubleshooting and Diagnostics Problems with the Replenishment System and Buffers Containing SDS When a vial is replenished the SDS foam may stick to the side of the vial The empty function draws air around the foam into the replenishment needle When filling this vial
83. Display the current logbook 17 Display sample information for sequences or single vials 4 Store method 11 Display the current sequence logbook 18 Access integration calibration and reporting settings Store sequence 12 Open the Preferences dialog 19 Access sequence templates 6 Load sequence 13 Name of current sequence 20 Access methods Change the layout to show the 14 Name of current method sampling area and diagram Agilent 7100 Capillary Electrophoresis System User Manual 85 5 No OT Porn Using the Agilent 7100 Capillary Electrophoresis System Working with th e CE Diagram Graphical User Interface Explanation CE control diagram DAD control diagram CE status Tray tray temperature Inlet lift loaded vial Outlet lift loaded vi Cassette temperature Components of the Agilent CE instrument in the CE diagram synonym dashboard are shown in Figure 17 Each component in the instrument is represented by an icon in the CE diagram or DAD diagram instrument Control Easy Sequence Sequence Queue Easy Sequence Setup sequence 5 Li DAD DEF_CEM 2 CE Idle ox y e Onn ou 18 WL BWRefWL RefBW mAU 2350 4 of of 11 SEE sal B 2350 4 3800 20 14 C 1920 4 of off 11 6 D 192 0 4 380 0 200 11 0 E 2600 4 of of 0 4 Figure 17 The CE and DAD diagram represents each component in the instrument High voltage power supply 15 Method steps and progress Internal pressure 16
84. E 4 Carefully pull the cassette out of the cassette guide See Figure 27 on page 123 Cassette Figure 26 Position of installed cassette NOTE The DAD detector is equipped with a white LED located near the outlet electrode This light will assist you when inserting the capillary Cassette Cassette guide 122 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis CE Figure 27 Removing the cassette Agilent 7100 Capillary Electrophoresis System User Manual 123 5 Using the Agilent 7100 Capillary Electrophoresis System Installing a Capillary in the Alignment Interface The following procedure describes how to install a capillary into an alignment interface A capillary must have an alignment interface installed before it can be installed into the capillary cassette in order to align the inner capillary with the light path of the diode array detector Material Needed to Install a Capillary in the Alignment Interface Capillary Corresponding alignment interface Capillary insertion tool separate item or part incorporated in cassette e Safety glasses WARNING Risk of injury of sharp capillary ends Sharp capillary ends can injure your eyes or skin Wear safety glasses when opening the cassette and handling the capillary to protect your eyes and body Alignment interfaces for the 7100 CE instrument differ fr
85. Hardware Information 7 Analog to Digital Converter CE Part Configuration Instrument 1 67100 Analog In 1000000 Figure 56 CE part configuration Agilent 7100 Capillary Electrophoresis System User Manual 187 7 188 Hardware Information Analog to Digital Converter Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System User Manual 8 Maintenance Standard Operating Procedures for Maintenance of Your Agilent CE Instrument 190 Overview of Maintenance 191 Early Maintenance Feedback EMF 194 Cleaning the Electrodes Pre punchers and Insulation Plate 196 Maintenance of the Replenishment System 216 Changing the Air Inlet Filter 222 Changing the Lamp 224 Cleaning the Instrument 228 This chapter describes how to maintain the CE instrument RE Agilent Technologies 189 Maintenance Standard Operating Procedures for Maintenance of Your Agilent CE Instrument 190 This chapter describes standard operating procedures SOPs for regular maintenance of your instrument Only perform maintenance on the Agilent CE instrument that is specified in this chapter Other maintenance or repairs must be done by personnel trained by Agilent Technologies Unauthorized maintenance may be dangerous and damages are not covered by warranty Maintenance tasks are supported by the Agilent Lab Advisor software or the Agilent Instrument Utilities software It is delivered with the 710
86. Installing the Instrument e Unpacking the Agilent CE Instrument 54 Installing the Instrument 57 In this chapter you will find information on how to unpack the Agilent CE instrument how to set up the Agilent CE instrument Information on how to install the Agilent ChemStation software or on how to install the Lab Advisor Instrument Utilities software can be found in separate documentation located on the respective software DVD RE Agilent Technologies 53 3 Installing the Instrument Unpacking the Agilent CE Instrument Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Unpacking the CE Instrument The Agilent CE instrument is heavy and should be carried by two people 1 Use the recessed handles on both sides to carry the Agilent CE instrument 2 Open the box in which the Agilent CE instrument was shipped 3 Pull the Agilent CE instrument out of
87. Method Set Error Method Identify Device Identify Device PL Balancing _ Switch UV Lamp on Figure 19 Menus in the DAD and CE diagram To trigger actions for an instrument component select the appropriate icon in the diagram A context menu including possible actions such as switching on the lamp appears Any instrument action triggered through such a menu does not change the current method To change values for a component select the appropriate icon A menu with three general items and parameter specific for the component appears Method A click here requests the current method from the instrument and opens the CE Method Setup dialog box for the CE part of the current method or the respective DAD part To set up a complete method refer to How to Use Your Agilent CE Instrument for Capillary Electrophoresis Separations on page 92 If closed with OK the possibly modified method is downloaded to the device If changes have been made an asterisk is added to the method status The method status is represented by a book icon in the top line of the dashboard Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Direct Control of Instrument Components The actions are performed immediately and the changes are reflected in the CE diagram Such actions include for example Switching the lamp on Controlling the sample tray Lowering the lift
88. System User Manual 217 8 Maintenance The Replenishment System Will Not Be Used for Some Time The replenishment system should be flushed first with water and then with isopropanol Please follow these steps 1 Open the Lab Advisor or Instrument Utilities software and start monitoring the respective instrument In the tools section select the CE Control screen and click on Change Bottles to release the pressure before you open the bottles Take out the electrolyte bottle and unscrew the cap The electrolyte bottle has a frit and tubing inside The frit must not get dirty Fill a minimum 300 mL of water into the electrolyte bottle Make sure the water is filtered by using a 0 25 um or 0 45 um filter depending on its viscosity Make sure the waste bottle is empty Cap the bottles tightly Choose Done in the Change Bottles dialog box Wait until the pressure has built up again To flush the internal tubing system from the electrolyte bottle to the waste bottle including all valves on this pathway select Flush Tubes During this step the replenishment needle itself is not flushed Set a flushing time of 2 minutes To clean the internal tubing system including the replenishment select Clean Tubes During this step the replenishment is used to fill and empty a vial several times Set the number of repetitions to 5 10 When requested to do so place a capped and empty vial into a tray position 11 Execute the clean level s
89. W RefWL RefBW mau CA 1920 4 3600 100 41 out Inlet B 2540 10 3600 100 16 2 8 cf 235 0 10 3600 100 17 253 Electrolyte Waste Actual Values Method Limits 0 0KV 0 0 KV pus 300 pA GoW 6 0W fr ale J Time 30 00 min OGE00020 0 00 0 00 une eo Figure 39 CE diagram with CE p mode activated Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Operation and Function in CE p Mode By selecting CE p mode some additional functions are available for control via the CE diagram which will assist in the operation of capillary gel electrophoresis CGE with highly viscous buffers This includes the ability to apply high pressure during preconditioning and postconditioning and the ability to use high pressure to introduce gel or samples to the capillary Preconditioning and Postconditioning When the instrument is operating in CE p mode the user can chose to use the High Pressure Flush command menu during preconditioning or postconditioning Figure 41 This means that high pressure will be applied to the capillary inlet vial which may be A set value 2 12 bar Raw external pressure up to 15 bar Allows pressurizing the inlet vial and or the outlet vial The external pressure up to 15 bar can be applied directly without reduction Agilent 7100 Capillary Electrophoresis System User Manual 143 5
90. ack of 100 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 The recommended maximum liquid level in the vials is 1 8 cm measured from the bottom of the vial see Figure 25 The minimum level depends on the type of liquid the vial is used for sample buffer or waste 5 mm Liquid level Be T4 mm Figure 25 Liquid level and lift offset for standard electrodes To touch the standard electrodes part number G7100 60007 the liquid level must comply with the minimum condition Liquid Level L Lift offset 5 mm up to electrode 1 mm guard band 1 cm See Figure 25 For short electrodes part number G7100 60033 the liquid level must comply with the minimum condition Liquid Level L Lift offset 10 mm up to electrode 1 mm guard band 1 cm To touch the capillary only the liquid level must comply with the condition for both types of electrodes Liquid Level L Lift offset 1 mm guard band 0 5 cm Sample Vials The level of sample in the vials depends on the injection technique used Please see the section Injection on page 103 Agilent 7100 Capillary Electrophoresis System User Manual 117 5 Using the Agilent 7100 Capillary Electrophoresis System Hydrodynamic Injection When using injection by pressure the sample level only needs to touch the capillary The minimum liquid level in the sample vials f
91. al you want to store in the data file is displayed below the lamp icon of the DAD tab of the instrument diagram Peak Width The peak width sets the optimum response time of your detector If you have similar peak widths in your electropherogram set it to the width of one of the peaks in your electropherogram If the peak widths are very different you can use the detector timetable to adjust the peak width setting during the analysis If your peak width setting is too narrow peaks might be not detected automatically This will lead to no spectra for the wider peaks being acquired and in case of peak dependent detection Agilent 7100 Capillary Electrophoresis System User Manual 107 5 108 Using the Agilent 7100 Capillary Electrophoresis System Spectra On the right hand side of the DAD Method Setup dialog an expandable page for advanced settings is visible Under Spectrum you can define at which points on a signal spectra will be taken and saved You can choose to acquire them e At the upslope apex and downslope of the peak e Allin a peak All spectra during analysis Every 2nd spectrum or e None You can combine this setting with a wavelength range By restricting the wavelength range to the range of your interest you can save some disk space the size of your data files will be smaller because the full spectrum is not stored peak height threshold avoids spectra that are taken from small peaks If spectra
92. alues slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Please introduce self made EMF counters to remind you to frequently clean the electrodes pre punchers and insulation plate Agilent 7100 Capillary Electrophoresis System User Manual 195 Cleaning the Electrodes Pre punchers and Insulation Plate 196 Buffer precipitates dust moisture or other solid material in the electrodes and pre punchers or under the insulation plate may cause arcing They can lead to current leakage buffer contamination or carry over Cleaning these parts regularly will prevent this The electrodes pre punchers and the insulation plate should be cleaned regularly Depending on the application cleaning frequency can be for example once a week or if peaks show unusual tailing or unusual peak shoulders Please be aware that the EMF counter analysis start lets you set customized limits for warnings Cleaning is necessary if arcing or excessive current leakage is observed Usage of creeping buffers such as urea may lead to higher cleaning frequency Steps for the cleaning procedure for electrodes pre punchers and the insulation plate Prepare the Agilent CE instrument for this task Remove the covers Access the electrodes Access the liquid handling module
93. am 158 Agilent 7100 Capillary Electrophoresis System User Manual Running the CEC Analysis 159 6 Troubleshooting and Diagnostics 161 CE Problem Solving 162 Power OnFail 162 Leaks 163 Blocked Replenishment Needle 165 Leakage Current 165 Broken Capillary 166 Contaminated Insulation Plate 166 Empty Injection Vial 167 Dirt in the Vials 167 Air Bubble in Injection Vial or Run Buffer Vials 167 Air Bubble in Capillary 167 Problems with the Replenishment System and Buffers Containing SDS 168 Agilent Lab Advisor Software 169 Configuration 169 7 Hardware Information 171 Identifying Instrument Components 172 7100 Capillary Electrophoresis Instrument Electronics 173 Firmware 176 Resident System 176 Main System 176 Firmware Updates 177 Diode Array Detector 179 DAD Control 180 Digital to Analog Converter 181 Changing the Fuses for the Power Supplies 182 Installing Drainage Tubing 183 External Water Bath for Tray Cooling Optional 184 Analog to Digital Converter 186 Agilent 7100 Capillary Electrophoresis System User Manual Contents Contents 10 Maintenance 189 Standard Operating Procedures for Maintenance of Your Agilent CE Instrument 190 Overview of Maintenance 191 Early Maintenance Feedback EMF 194 EMF Counters 194 Using the EMF Counters 194 Setting the EMF Limits 195 Cleaning the Electrodes Pre punchers and Insulation Plate 196 Prepare the Agilent CE Instrument 197 Remove the Covers 198 Accessing the Electrodes
94. ample a shock hazard when the cover is opened and the module is connected to the power Make sure that it is always possible to access the power plug Remove the power cable from the instrument before opening the cover Donot connect the power cable to the instrument while the electronic box in the back of the instrument is opened or side panels are removed Incorrect line voltage on the instrument Shock hazard or damage to your instrument can result if the device is connected to a line voltage that is higher than specified Connect your instrument to the specified line voltage 18 Agilent 7100 Capillary Electrophoresis System User Manual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In the case of an emergency it must be possible to disconnect the instrument from the power line at any time Make sure the power connector of the instrument can be easily reached and unplugged Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are delivered with the instrument The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of a ground connection or use of an unspecified power cord The absence of a ground connection or the use of an unspecified
95. ample vial A pressure is applied for a certain time to introduce the sample in the capillary The system constantly controls the pressure and corrects for the rise time effects of valves and the compressibility of air When pressure is applied the pressure to the sample vial is increased gradually to its setpoint after which the pressure decreases gradually to approximately a fifth Then a correction time is inserted after which the pressure decreases gradually to atmospheric pressure This results in accurate and reproducible injection as well as exceptional injection linearity Injection by pressure is the most frequently used injection technique There are no differences in injection concentration for molecules with different mobilities as in electrokinetic injection With viscous buffer system the flush command can be used However injection of sample to the capillary at this high pressure is not monitored by the self correcting injection algorithm This sample application to the capillary is therefore less reproducible To compensate for this internal standards might be useful in quantitative analysis Agilent 7100 Capillary Electrophoresis System User Manual 103 5 Using the Agilent 7100 Capillary Electrophoresis System Electrokinetic Injection For electrokinetic injection the inlet buffer vial is replaced with the sample vial A voltage current or power is applied for a certain time that causes the sample to migrate into the capil
96. ange it to a valid address immediately unless you plan a direct LAN connection to stand alone PC Agilent 7100 Capillary Electrophoresis System User Manual 67 4 68 LAN Configuration Table 19 Using Default parameters IP address 192 168 254 11 Subnet mask 255 255 255 0 Default gateway Not specified Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the CE instrument must be in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the CE instrument He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the 7100 CE instrument is wired to the PC directly for example using a cross over cable or a local hub that is separate from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the 7100 Capillary Electrophoresis instrument are not cleared automatically If not changed by the user they are still available when you switch back to the mode Using Stored Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration 4 Link Configuration Selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex mo
97. ate the instrument configuration O Suppress this message Figure 12 Instrument configuration during ChemStation startup Agilent 7100 Capillary Electrophoresis System User Manual 81 5 Usingt 82 he Agilent 7100 Capillary Electrophoresis System nent Starting the Agilent ChemStation Software the First Time Starting the Agilent ChemStation software the first time will lead to the following message Empty configuration x A There is no configuration available For this instrument Do you want to auto configure it Figure 13 Configuration at first start of ChemStation Please select Yes The following dialog will allow you to configure the instrument Please provide an IP address or host name in the subsequent dialog Check the respective radio button and type in either the IP address or the host name of your instrument Communication settings Communication settings IP Address Figure 14 Request of IP address or host name The auto configuration process will detect an instrument at the IP address or host name you provide You can confirm the instruments identity with the serial number Please select Configure if you would like to connect to a different instrument Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Instrument Configuration Configurable Modules Selected Modules Agilent CE G7100A Agilent CE G7100A Auto
98. ation 7 enter sample name 1 mM pHAP and click Run method to start the analysis A typical electropherogram will show a significant peak at a migration time of around 3 minutes Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Method Parameter CE Default Settings Vials e Inlet home vial 4 e Outlet home vial 5 Cassette Temperature e 20 0 C High Voltage System Enable high voltage Voltage 0 0 V will ramp up in accordance with timetable e Current 300 uA Power 6 0 W Low current limit OFF Stoptime 10 0 minutes Posttime OFF Replenishment Replenishment and preconditioning parallel processing Noreplenishment used Agilent 7100 Capillary Electrophoresis System User Manual 253 10 Appendix Preconditioning CE Conditioning Flush inlet 1 outlet 8 for 5 minutes Wait 5 minutes Flush inlet 2 outlet 8 for 5 minutes Flush inlet 6 outlet 8 for 10 minutes CE Injection Inject by pressure 50 mbar 4 seconds Timetable Change voltage 0 2 minutes 30 kV Postconditioning No postconditioning used 254 Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Method Parameter DAD Signals Signal Bw nm Reference Bw Use signal A Yes wavelength 325 Bandwidth 10 no Reference Use signal B Yes wavelength 192 Bandwidth 4 no Reference Use signal C Yes wavelength 235 Bandwidth 10 no Reference Peak width
99. ation 86 Menus in the CE and DAD Diagram 88 System Vialtable 90 How to Prepare your Agilent CE Instrument for Analysis 91 Capillary Electrophoresis CE 92 How to Use Your Agilent CE Instrument for Capillary Electrophoresis Separations 92 Agilent 7100 Capillary Electrophoresis System User Manual 7 Contents Agilent CE Method Parameters 92 Vials 96 Cassette Temperature 97 High Voltage System 98 Posttime and Stoptime 100 Replenishment 100 Preconditioning 101 Injection 103 Timetable 105 Detector Parameters 107 Switching the Lamp On 110 Preparing the Replenishment System if Needed 111 Preparing Vials for Buffer and Sample 115 Installing a Capillary in the Alignment Interface 124 Installing a Capillary in an Empty Capillary Cassette 128 Inserting the Cassette 132 Fraction Collection 135 Capillary Electrophoresis Plus High Pressure CE p 136 Requirements 136 Outlet Lift Connection to the Tubing System 138 Preparing the Agilent CE Instrument for High Pressure Use 140 Operation and Function in CE p Mode 143 Injection Options with CE p 146 Timetable Options with CE p 146 CE p Control Using the CE Diagram 147 Capillary Electrochromatography CEC 148 How to Use Your Agilent CE Instrument for Capillary Electrochromatography 148 Preparing the Agilent CE Instrument for High Pressure Use 150 CEC Specific GUI Explanation 153 Injection Options with CEC 156 Timetable Options with CEC 157 CEC Control Using the CE Diagr
100. avo di alimentazione collegato il modulo riceve parzialmente energia Gli interventi di riparazione o manutenzione del modulo possono provocare lesioni personali come scosse elettriche nel caso in cui il coperchio sia aperto e il modulo sia collegato all alimentazione Verificare che sia sempre possibile accedere alla presa di alimentazione Scollegare il cavo di alimentazione dallo strumento prima di aprire il coperchio Non collegare il cavo di alimentazione dello strumento se l alloggiamento dell elettronica sul retro dello strumento aperto o se i pannelli laterali sono stati rimossi ATTENZIONE Tensione di linea non corretta nello strumento Se gli strumenti vengono collegati a una tensione pi elevata di quella prevista esiste il rischio di danneggiarli e Collegare lo strumento alla tensione di linea specificata Agilent 7100 Capillary Electrophoresis System User Manual 33 2 Site Requirements and Specifications AVVERTENZA Presa di alimentazione inaccessibile In caso di emergenza deve essere possibile scollegare lo strumento dalla rete elettrica in qualsiasi momento Accertarsi che il connettore di alimentazione dello strumento sia accessibile e possa essere scollegato facilmente Verificare che lo spazio dietro la presa di alimentazione dello strumento sia sufficiente per consentire lo scollegamento del cavo Cavi di alimentazione ATTENZIONE Lo strumento viene fornito con cavi d
101. ble to connect to the instrument under these circumstances An instrument restart by switching the power off and on again boots with complete firmware loaded During a main firmware download see Firmware on page 176 the instrument shows a red blinking LED Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Starting Your Computer and the Agilent ChemStation Software After you have switched on your computer and it has booted up start the Agilent ChemStation by double clicking the Instrument Online icon in the Agilent ChemStation menu In order to control the 7100 Capillary Electrophoresis instrument the ChemStation revision must be B 04 02 or higher We recommend you install the Lab Advisor software revision B 01 03 or higher If you have problems starting the Agilent ChemStation check that the software has been properly installed During startup the ChemStation might ask you to update the connection settings see Figure 12 You might suppress this message in the future If you want to update the instrument configuration select Yes Typically an instrument configuration must be updated for first time use of the software changes in the IP address or changes in the mode of usage Please refer to the ChemStation documentation and online help for details on installation and configuration of the 7100 CE instrument Instrument configuration 2 J Do you want to upd
102. book entry is created The run is stopped Pressure is released High voltage application is stopped Agilent 7100 Capillary Electrophoresis System User Manual 163 6 Troubleshooting and Diagnostics If the leak sensor is activated or you think there is a leak in the instrument 1 Immediately switch off the Agilent CE instrument and the external water bath if connected Disconnect the line power cord Open the lid Remove the front cover as described to diagnose the cause of leak c A N Dry the leak sensor using a tissue The error will stay active for the ChemStation or Lab Advisor even after corrective action and drying Please acknowledge the error by clicking Clear error in the Lab Advisor or switching the power knob in the top right corner of the CE diagram of the ChemStation Possible Reasons for Leaks and Appropriate Action for Spilled Liquid from Other Sources If you have spilled some liquid into the Agilent CE instrument it is sufficient to thoroughly clean and dry the Agilent CE instrument and the leak sensor Leaking Water Bath Tubing Inside the Agilent CE Instrument e Call Agilent Technologies Do not use the Agilent CE instrument until Agilent Technologies has repaired it Leaks in Replenishment Tubing or Valves Call Agilent Technologies Reason Unknown f you can not trace the reason for the leak call Agilent Technologies There may be an instrument problem 164 Agilent 7
103. bout 1 hour If the warm up time of the lamp is too short a baseline drift in the electropherogram may occur 110 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Preparing the Replenishment System if Needed During multiple analyses the composition of the buffer can change One way to refresh the buffer is replenishment The replenishment system automatically empties and refills the buffer vials in the tray with new buffer from the electrolyte bottle Typically replenishment is done before every or after every 3 to 4 runs depending on the stability and volume of the running buffer If you do NOT want to use the replenishment system you still have to put empty bottles electrolyte and waste into the replenishment system at the bottom of the instrument The bottles are also used as a pressure and vacuum reservoir Missing bottles lead to pressure and or vacuum error messages WARNING System under pressure Pressure is applied to the bottles Only use bottles with part number 9300 1748 or 5042 6478 with this instrument These bottles have a protective coating If you want to use the replenishment system you have to fill the electrolyte bottle with buffer Use about 100 mL to make sure the frit is submerged in buffer Do not fill more than 400 mL buffer into the bottles to ensure the system can work properly When using buffers containing detergent for e
104. cal contact Injection Options with CEC The installed high pressure supply may also be used for injection purposes using the Timetable option in the Injection field of a method setup From this menu you may select the High pressure flush command during injection with the same parameter choices as those available during preconditioning This may be used to apply a pressure injection while using a replaceable gel filled capillary Please note that the external pressure is not as precise as the 50 mbar injection pressure Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Timetable Options with CEC The installed high pressure supply may also be used in the general timetable of a method setup The Pressure command is extended by the External pressure functionality see Figure 49 on page 157 Pressure iit Intemal 0 mbar Flush 850 mbar intemal External Figure 49 Pressure options in CEC mode Timetable controlled changes in the pressure settings are OFF Pressure usage is switched off Internal pressure 100 mbar to 100 mbar Can be applied to the inlet vial Flush Standard flush procedure is applied with an internally generated pressure of approximately 950 mbar External Allows pressurizing the inlet vial and or the outlet vial External pressure is reduced to the set value between 2 and 12 bar and applied in a controlled manner
105. ce 260 Agilent Technologies on Internet 261 This chapter provides additional information regarding safety legal considerations and Agilent information on the Internet This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards RE Agilent Technologies 231 10 Appendix General Safety Information General The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 232 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that c
106. ce with stabilizing pins to you 2 Hold the bottom part for example with both of your thumbs and slide the top part 8 mm 0 3 inch gently towards you 3 Lift and remove the top part Do not use excessive force to open the cassette This might break parts inside the cassette 128 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Panillar Elastrankinrneie Lap il ary Eiectrophoresis LE Cassette front Cassette back Figure 32 Opening the cassette lock Installing the Capillary 1 Hold the capillary at the alignment interface 2 Insert the alignment interface into the alignment interface holder on the cassette Make sure the flat side of the alignment interface is at the outer edge of the cassette see Figure 33 on page 130 3 Make sure the capillary slides into the capillary guiding slit and a guiding hook above the area of the alignment interface 4 Wind the capillary around the reel in the center of the capillary cassette The pins surrounding the reel have grooves to secure the capillary Agilent 7100 Capillary Electrophoresis System User Manual 129 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophor 5 Start with the inside of the upper pin to prevent the capillary from being squeezed when the cassette is closed Capillary lock Alignment interface Capillary insertion tool Capillary Fi
107. ch Certaines op rations de r paration ou d entretien sur le module peuvent occasionner des blessures par exemple l lectrocution si le capot est ouvert et que le module est branch Veillez ce que la prise d alimentation lectrique reste tout moment accessible D branchez le c ble d alimentation de l instrument avant d ouvrir le capot Ne branchez pas le c ble d alimentation sur l instrument si le boitier lectronique l arri re est ouvert ou si les panneaux lat raux sont enlev s AVERTISSEMENT Tension de secteur incorrecte au niveau de l instrument Il existe un risque d lectrocution ou de dommages l instrument en cas de raccordement une ligne d alimentation de tension sup rieure celle sp cifi e Connectez l instrument la tension indiqu e 28 Agilent 7100 Capillary Electrophoresis System User Manual ATTENTION Site Requirements and Specifications Prise d alimentation inaccessible En cas d urgence il doit tre possible de d brancher tout moment l instrument du secteur Veillez faciliter l acc s au connecteur d alimentation de l instrument et permettre de le d brancher facilement Maintenez un espace suffisant derri re la prise d alimentation de l instrument pour pouvoir d brancher le c ble Cables d alimentation AVERTISSEMENT Diff rents c bles d alimentation sont livr s avec l instrument L extr mit femelle est la m me pour t
108. ckage of the needle Water in vial 49 can change the buffer composition inside the replenishment needle and the adjacent tubing over time due to diffusion This effect might influence the reproducibility of analysis Therefore we recommend using buffer Inserting the Vials The tray of the Agilent CE instrument is used for both buffer and samples The CE diagram of the Agilent ChemStation controls the tray 1 From the CE diagram open the control menu by clicking the tray icon The dialogue Get Vial allows you to enter the vial number or tray position Wait until the tray has stopped moving Open the tray door Cb MN Access the vial which is in the front The number is engraved in the sample tray For safety reasons the tray can only be moved by the Agilent ChemStation when the tray door is closed 6 Click Done after you have accessed all the vial positions you need Agilent 7100 Capillary Electrophoresis System User Manual 119 5 120 Using the Agilent 7100 Capillary Electrophoresis System The tray will perform a complete turn every time the tray door is closed in order to read out the occupied tray positions with an internal vial sensor Methods will only run if all specified vials are present The vial sensing procedure might also be delayed if any operation prevents the vial handler temporarily from turning Manual Operation If you want to operate the tray manually or access positions of the tray it is recommende
109. d Setup Agilent 7100 Capillary Electrophoresis System User Manual 93 Setup Method Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis CE SY DAD CE X Instrument Curves 33333333 94 Figure 21 DAD Method Setup Selecting 0K transfers the parameters to the instruments and closes the dialog Choosing Apply only transfers the current parameter to the instrument Save the edited method if appropriate If you do not save the method to the hard disk this changed method is executed when choosing Run Method When you refer to a method in a sequence the method is always loaded from the disk So if you did not save your changes using Save Method the original method from the hard disk is used Method parameters are also available if Method Edit Entire Method is chosen This dialog guides you through further parameters associated with a method such as Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis CE Method Information Instrument Acquisition Data Analysis Run Time Checklist The book icon in the software toolbar is a further alternate entry point for method editing When you click the icon you can access menu items to set method parameters Agilent 7100 Capillary Electrophoresis System User Manual 5 95 5 Using the Agilent 710
110. d arcing which disturbs applications and may damage the instrument 5 Carefully insert the replenishment pre puncher Tighten the screw to secure the pre puncher The pre puncher will assume the correct position as the screw is tightened The replenishment pre puncher is not connected to a tube Agilent 7100 Capillary Electrophoresis System User Manual 209 8 Maintenance Cleaning the Electrodes Pre punchers and Insulation Plate Reinstalling the Electrodes and the Insulation Plate 1 Reinstall one electrode in the outlet electrode position on the insulation plate 2 Thread the electrode into the outlet position on the insulation plate by hand first 3 Tighten the electrode with the 12 mm hexagonal socket screwdriver Do not overtighten Do not bend the electrodes 4 Holding the insulation plate by the cassette holder slide the insulation plate under the detector 1 then tilt it up into place 2 and push it completely to the left 3 3 Left shift 2 Rotation 1 Insert Figure 67 Installation of the insulation plate 210 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 5 The insulation plate should be laying flat in position The insulation plate is correctly positioned under the detector if the top part of the outlet electrode is fits through an oval cut out NF Figure 68 Alignment of the detector 6 Secure the insulation plate by reinstalling the two slotte
111. d metal screws 7 Reconnect the blue ground wire and fasten the metal screw 8 Insert the inlet electrode into the ring connector of the red high voltage wire Thread the inlet electrode and the ring connector into the insulation plate by hand then tighten the inlet electrode with the 12 mm hexagonal socket screwdriver Do not overtighten Agilent 7100 Capillary Electrophoresis System User Manual 211 8 Maintenance Cleaning the Electrodes Pre punchers and Insulation Plate 9 Push the liquid handling module back into the instrument as shown in Figure 70 on page 213 To release it from the front position slightly more force is needed Carefully move the liquid handling module completely into a position that allows fixation Please note the metal nose from the liquid handling module which has to fit in a hoe of the back metal sheet as shown in Figure 69 on page 212 Metal pin Connectors for water pressure drainage Figure 69 Rear side of liquid handling module NOTE Take care not to damage tubes and flat ribbon cables on the right hand side of the liquid handling module Take care that the connectors on the left hand side for example for the drainage fit into their cut out in the back 212 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Cleaning the Electrodes Pre punchers and Insulation Plate 10 Tighten the slotted screws in the front of the liquid handling module 3 F
112. d that you remove the vials from the lifts first This avoids the need to fill the positions for those vials in the tray again Use the Unload option in the individual lifter context menus to remove the vials from the lifts During the run you can access the vial tray any time the software is not trying to access it If you try to access the tray during the run you cannot remove the vials from the inlet and outlet lifts Therefore you have to be careful not to fill the positions for those vials in the tray again Check in the CE diagram which vials are currently located in the lifts If you insert a vial in a tray position that is already occupied but where the vial is currently loaded to a lifter a message will request that you remove the duplicate vial A duplicate vial is brought to the front position Positioning Vials for Sample and Buffer 1 Note the positions of the vials in the table see System Vialtable on page 90 2 Fillthe sample into a sample vial and put the vial in the tray for example in position 7 This position is used in the Sample Information screen or the Sequence Table screen later Fill at least two vials with the buffer you want to use for your analysis 4 Position those vials in the sample tray For example use positions 5 and 6 Write down the positions When creating the Agilent CE method you must enter the positions of the buffer vials as CE Home Values for the inlet home and outlet home vial After t
113. del presente manuale implica la violazione degli standard di sicurezza della progettazione della produzione e dell uso previsto dello strumento Agi lent Technologies non riconosce alcuna responsabilit per eventuali danni risultanti dal mancato rispetto delle istruzioni fornite Informazioni generali Questo strumento classificato come facente parte della Classe di Sicurezza I provvisto di terminale di messa a terra ed stato prodotto e collaudato in conformit con gli standard di sicurezza internazionali Funzionamento Prima di attivare l alimentazione seguire le istruzioni della sezione relativa all installazione Inoltre osservare quanto segue Non rimuovere i coperchi dello strumento mentre in funzione Prima di accendere lo strumento collegare tutti i terminali di messa a terra le prolun ghe i trasformatori automatici e gli altri dispositivi ad esso collegati alla messa a terra di protezione tramite la speciale presa L eventuale interruzione del collegamento alla terra di protezione pu provocare scosse elettriche che possono causare lesioni gravi alle persone Se si sospetta che lo strumento sia rimasto privo di protezione scollegarlo subito e rimuoverlo dall uso Verificare che in caso di sostituzione dei fusibili vengano utilizzati solo quelli con la corrente nominale richiesta e del tipo specifico normale ad azione ritardata e cos via Evitare l uso di fusibili riparati e il corto circuito delle sedi dei
114. des In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the CE instrument is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the CE instrument Table 20 Link configuration switches SW3 SW4 SW5 Link configuration OFF Speed and duplex mode determined by auto negotiation ON OFF OFF Manually set to 10 Mbps half duplex ON OFF ON Manually set to 10 Mbps full duplex ON ON OFF Manually set to 100 Mbps half duplex ON ON ON Manually set to 100 Mbps full duplex Agilent 7100 Capillary Electrophoresis System User Manual 69 4 LAN Configuration Storing the Settings Permanently with Bootp If you want to change parameters of the CE instrument using Bootp follow the instructions below 1 2 70 Turn off the CE instrument Change the CE instrument s settings for the configuration switch to Bootp amp Store mode see Table 18 on page 65 Start the Agilent Bootp Service see Agilent Bootp Service on page 75
115. e Agilent 7100 Capillary Electrophoresis System 5 Using an Injection Program The injection table is used for advanced injection tasks such as Injection from different vials for example ITP Multiple injection modes for example spiking Injecting a buffer plug after the sample to prevent sample loss after applying voltage The online help provides more information on this Timetable In the CE Timetable present on the right hand side of the CE Method Setup dialog you can choose to set and change values for the following parameters at a certain time during the run Voltage Current Power Pressure Inlet vial Outlet vial Cassette temperature Lower alarm limit for current High pressure only in CEC mode Time programming of these parameters can be useful for example to Define gradients for voltage or temperature Change the buffer during the analysis inlet and outlet Apply pressure during the analysis Change the outlet vial during analysis Change the alarm limit for current for example when changing the buffer system during the analysis Agilent 7100 Capillary Electrophoresis System User Manual 105 5 Using the Agilent 7100 Capillary Electrophoresis System Instrument Curves Using a further tab in the CE Method Setup dialog box you can choose to monitor and store the following CE specific raw data If selected they are stored in addition to the detector signals defined
116. e Agilent CE instrument showing the current status of the system as a whole Each icon represents one component in the system Common Software Toolbar The software tool bars in the Agilent ChemStation offer a group of commonly used functions The software tools bars visible in the Method and Run Control View are shown in Figure 17 on page 86 To change values for a component you must choose the appropriate icon This will bring up the menu The menu may comprise sets of items for changing parameters in your method for control of the instrument and for help The tool bars associated with the Data Analysis View and Report Layout view look similar and are described in the online help and the ChemStation documentation For more assistance click Help in the menu bar 84 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 No Nith the Software Toolbar 17 15 18 Sequence templates Methods Instrument Z pata Analysis era DE ES f Report Layout a g raten Sampie Name Sangi Jo Ce ao de p Vial pes Ju Figure 16 Components of the common software toolbar of the ChemStation 1 Work with sequences 8 Change the layout to show two online 15 Display of elapsed and overall run plots time 2 Work with single vial analysis 9 Change the layout to show two online 16 Status display plots and the current log book 3 Load method 10
117. ectrolyte bottle has a frit and tubing inside The frit must not get dirty Fill a minimum 300 mL of buffer into the electrolyte bottle Make sure the buffer is filtered by using a 0 25 um or 0 45 um filter depending on its viscosity Make sure the waste bottle is empty Cap the bottles tightly Choose Done in the Change Bottles dialog box Wait until the pressure has built up again To flush the internal tubing system from the electrolyte bottle to the waste bottle including all valves on this pathway select Flush Tubes During this step the replenishment needle itself is not flushed Set a flushing time of 0 3 minutes To clean the internal tubing system including the replenishment select Clean Tubes During this step the replenishment is used to fill and empty a vial several times Set the number of repetitions to 5 10 When requested to do so place a capped and empty vial into a tray position The involved volume of the replenishment system is about 5 mL A 10 time flush is sufficient The flush time depends primarily on the viscosity of your buffer Therefore choose a short time and repeat flush tubes if necessary otherwise it is possible that the buffer is flushed completely into the waste bottle This protocol suggests a combination of flush tubes first and a subsequent clean tubes step with comparably low volume consumption for a high exchange efficiency and low buffer consumption Agilent 7100 Capillary Electrophoresis
118. ed Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 A cycle consists of 1 Submerging the short needle into the cleaning liquid and briefly sucking cleaning liquid into the short needle 2 Removing the short needle from the cleaning liquid and sucking a slug of air into the short needle The alternate slugs of liquid and air exit the level sensing tubing into the waste bottle The main screen reply line counts the cycles as they take place You can check for proper function afterwards with the IRP level sensing test from the injection replenishment system tests or by performing buffer replenishment in a vial This function is designed to prevent a gradual build up of buffer crystals Large solid plugs of precipitate which have been allowed to form in the short needle may not be removable using this function In such cases we recommend exchanging the restrictor capillaries and or the replenishment needle by personnel trained by Agilent Technologies If the replenishment system will not be used for some time the level sensor should be cleaned with water Subsequently the replenishment system should be cleaned with water and then with isopropanol by the Flush Tubes and Clean Tubes operations Agilent 7100 Capillary Electrophoresis System User Manual 221 Maintenance Changing the Air Inlet Filter 222 Objective The following procedure is used to replace the air inlet filter The installed air pump uses
119. eep the detection window clean Donottouch the capillary at the detection window or the capillary tips where the polyimide is burnt off Clean the detection window and the capillary tips as described Put the insertion tool on a flat surface for example your lab bench Colored sleeve Alignment interface gu Capillary insertion tool Figure 28 Alignment interface and insertion tool Agilent 7100 Capillary Electrophoresis System User Manual 125 5 Using the Agilent 7100 Capillary Electrophoresis System 3 Put the alignment interface on the insertion tool and press down firmly Press only on the outer ring to release the spring that secures the capillary in the alignment interface Four plastic pins in the alignment interface belong to a feather driven mechanism to fix the capillary tight in an optimal position for the light path Press these pins with the alignment tool when inserting a capillary Insertion attempts of capillaries without this can damage capillary ends and may lead to dissatisfying results q Colored plastic stop N Figure 29 Alignment interface and insertion tool 4 Slide the capillary through the opening in the direction of the arrow printed on the interface 5 The capillary has a plastic stop fitted Pull the capillary through the alignment interface until the stop is reached 6 While still slightly pulling the capillary stop against the alignment interface and release
120. emale adapter and PTFE tubing are part of the shipment The Agilent 7100 Capillary Electrophoresis System is designed to operate in a typical electromagnetic environment where RF transmitters such as mobile phones should not be used in close proximity Agilent 7100 Capillary Electrophoresis System User Manual 21 2 Site Requirements and Specifications Performance Specifications Table3 Performance specifications for the Agilent 7100 Capillary Electrophoresis System Type Specification Comments Safety features Control and data evaluation Communication Extensive diagnostics error detection and display current leak detection low current limit Liquid leak sensor safety sensors on door and cover disabling high voltage vial sensor Agilent ChemStation under Windows XP SP3 LAN controller area network CAN RS 232C 0 to1 V APG remote ready start stop and shut down signals USB future use Analog out and in GLP features Early maintenance feedback EMF electronic records of maintenance and errors 22 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Anforderungen an den Aufstellungsort Eine geeignete Umgebung ist wichtig f r die optimale Leistungsf higkeit des 7100 Kapillarelektrophorese Ger ts Pr fen Sie sorgf ltig ob der von Ihnen gew hlte Aufstellort die nachfolgenden Anforderungen erf llt bevor Sie mit der Installation beginnen
121. en posici n vertical Entorno El instrumento funcionar dentro de las especificaciones de temperatura ambiente y humedad relativa descritas en la Tabla 10 de la p gina 41 PRECAUCI N Condensaci n dentro del m dulo La condensaci n da ar la electr nica del sistema No guarde traslade ni utilice el m dulo bajo condiciones en las que las fluctuaciones de temperatura pudieran provocar condensaci n dentro del m dulo Si el traslado del m dulo se realiz bajo condiciones ambientales fr as mant ngalo en su caja hasta que alcance lentamente la temperatura ambiente para evitar problemas de condensaci n 40 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Especificaciones fisicas Tabla 10 Especificaciones f sicas Tipo Especificaciones Comentarios Peso 35 kg Dimensiones 350 x 510 x 590 mm anchura x profundidad x altura Voltaje de l nea 100 240 V CA x 1096 Capacidad de amplio rango Frecuencia de l nea 50 o 60 Hz 5 Consumo de corriente 350 VA 300 W 1024 BTU h M ximo Temperatura ambiente 5 40 C operativa Temperatura ambiente De 40 a 70 C no operativa Humedad Por debajo del 80 a 31 C Sin condensaci n Altitud operativa Hasta 2000 m Refrigeraci n externa max 0 5 bares max 50 C Ba o Maria Presi n externa 2 12 bares Aire o nitr geno sin aceite Est ndares de seguridad IEC Categor a de instalaci n Il S lo para uso dentro de
122. ensing operation to clean the replenishment needle entirely 12 Repeat all steps starting with number 2 and use isopropanol instead of 218 water Empty the waste bottle whenever you fill or refill the electrolyte bottle Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Further Action If the instrument cannot build up pressure or a vacuum after performing the above steps check the following 1 Proper seal of the electrolyte bottle 2 Proper seal of the waste bottle 3 Tight fittings If none of these reasons apply call Agilent Technologies Operations for Replenishment Maintenance The following operations can be started from the ChemStation Lab Advisor or Instrument Utilities software From the ChemStation they can be done by clicking the replenishment vial It is a vial located between the replenishment bottles in the CE dialog From the Lab Advisor or Instrument Utilities software start monitoring visit the Tools section and the CE Control screen Flush Tubes The Flush Tubes operation directly flushes water buffer or other cleaning solutions from the electrolyte bottle to the waste bottle During this task a reduced pressure is utilized in the electrolyte bottle The user can set a time for this operation The volume in the tubing system from the electrolyte bottle and the waste bottle is about 5 mL It is recommended that you flush at least with 10 fold volume of fresh buffer The exchange
123. ente basso Sensore per il rilevamento di perdite di liquidi sensori di sicurezza sullo sportello e il coperchio per disabilitare l alta tensione sensore per vial Agilent ChemStation per Windows XP SP3 Controller LAN rete area controllore RS 232C Da0alV APG remoto segnali di pronto avvio arresto e interruzione ready start stop e shut down USB per un uso futuro Uscita e ingresso analogici Avviso di manutenzione preventiva Early maintenance feedback EMF registro elettronico della manutenzione e degli errori Agilent 7100 Capillary Electrophoresis System User Manual 37 2 Site Requirements and Specifications Requisitos de las instalaciones Es importante disponer de un entorno adecuado para lograr un 6ptimo rendimiento del sistema de electroforesis capilar 7100 Antes de comenzar la instalaci n compruebe minuciosamente que el lugar elegido cumple los requisitos citados a continuaci n Consideraciones sobre la corriente ADVERTENCIA La fuente de alimentaci n del instrumento tiene un amplio rango de capacidades y admite cualquier voltaje de linea est ndar en el rango especificado en la Tabla 10 de la p gina 41 Por lo tanto no hay ning n selector de voltaje en la parte posterior del detector Mientras el cable de alimentaci n est conectado el m dulo sigue recibiendo corriente aunque est apagado Las tareas de reparaci n o mantenimiento del m dulo entra an riesgos de lesiones personal
124. eplenishment During multiple analyses the composition of the buffer can change for example the pH due to electrolysis A simple way to refresh the buffer is to access different buffer vials in the tray which might be user vials in the method that are assigned to a certain tray position in the sequence table This is more useful for example during method development Another way to change the buffer is replenishment Replenishment can be programmed via the topmost table in the right side of the CE Method Setup screen It is done either in parallel or serial to the subsequent conditioning operation The associated timetable can be used to empty fill or replenish vials to a certain height The replenishment system automatically empties and refills the buffer vials in the tray with new buffer from the electrolyte bottle Therefore the number of tray positions occupied by home vials is reduced and a maximum of sample vials can be loaded Typically replenishment is done after every 3 or 4 runs Depending on the stability of the running buffer it is sometimes necessary to do it before each run If you want to use the replenishment system prepare it as described in Preparing the Replenishment System if Needed on page 111 Using parallel replenishment you can decrease the total time needed for your analysis as replenishment and preconditioning are done at the same time When using this feature you have to make sure that vials used i
125. eplenishment needle Replenishment lift IE ER Figure 62 Inlet and outlet lifts replenishment lift and replenishment needle Do not touch or bend the replenishment needle when handling the pre punchers WARNING Risk of injury due to sharp tips The pre punchers have very sharp tips Be careful when touching the tips of the pre punchers Agilent 7100 Capillary Electrophoresis System User Manual 203 8 Maintenance 1 Remove the plastic screw which secures the pre punchers using a Pozidriv screwdriver Teflon tubing Inlet pre puncher Screw plastic Figure 63 Plastic screw of the pre puncher 2 The pre puncher has a hole which can be used to gently lever it out using a paper clip Carefully pull the pre puncher out of the inlet outlet and replenishment lift 3 Unscrew the teflon inlet pre puncher tubing labeled 7 on the tubing the outlet pre puncher tubing labeled 8 The pre puncher for the replenishment lift is not connected to a tube The outlet lifter tubing labeled 8 might be physically disconnected from the pressure system This status is advantageous in case no high pressure applications are used This connection is required for applications in CE p and CEC mode The teflon tube does NOT need to be removed from the pre punchers for cleaning If the connection between the tube and the pre puncher is not tight since the tube became wider shorten the tube and shift it on the
126. er to suppress bubble formation while avoiding hydrodynamic flow due to a pressure differential How to Use Your Agilent CE Instrument for Capillary Electrochromatography Requirements External pressure supply lt 15 bar of oil free air or nitrogen Push fit connector and gas line supplied in startup kit Outlet lift connection to the tubing system For external pressure supply a male adapter fitting the instrument s female adapter and PTFE tubing 1 8 OD x 1 16 ID PTFE tubing are part in the accessory kit of the instrument Please add a suitable connector to the external pressure supply The inlet for the external high pressure gas is situated at the back of the instrument 148 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography CEC In connector Out connector External pressure connector Drainage Figure 43 External high pressure gas inlet Agilent 7100 Capillary Electrophoresis System User Manual 5 149 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography CEC Preparing the Agilent CE Instrument for High Pressure Use 1 Ensure that the gas source is switched off prior to connection 2 Connect the push fit connector and gas line to the receptor on the rear of the Agilent CE instrument Connect the gas line to the source 4 Switch on the gas s
127. erente y est dise ado acorde con los enchufes de cada pa s o regi n Conexi n a tierra inexistente o uso de un cable de alimentaci n no especificado La falta de toma de tierra y el uso de un cable de alimentaci n no especificado pueden provocar electrocuci n o cortocircuitos No utilice nunca el instrumento con una toma de corriente sin conexi n a tierra No utilice nunca un cable de alimentaci n distinto del suministrado por Agilent Technologies dise ado para su regi n ADVERTENCIA Uso de cables no suministrados El uso de cables no suministrados por Agilent Technologies puede producir lesiones personales o da os en los componentes electr nicos e Para asegurar un funcionamiento correcto y el cumplimiento de los reglamentos de seguridad o de compatibilidad electromagn tica no utilice nunca cables distintos de los suministrados por Agilent Technologies Agilent 7100 Capillary Electrophoresis System User Manual 39 2 Site Requirements and Specifications Espacio en el banco Las dimensiones y el peso del instrumento facilitan su colocaci n sobre pr cticamente cualquier mesa de laboratorio Deben dejarse unos 2 5 cm libres a cada lado y unos 8 cm en la parte posterior para la circulaci n del aire y las conexiones el ctricas En caso de colocar el Instrumento de Electroforesis Capilar Agilent 7100 en un banco confirme que ste pueda soportar el peso del sistema El instrumento se debe utilizar
128. erver The parameters obtained become the active parameters immediately They are not stored in the non volatile memory of the 7100 CE Therefore the parameters are lost with the next power cycle of the 7100 CE Bootp Active Server Parameter Figure 3 Bootp principle Agilent 7100 Capillary Electrophoresis System User Manual 65 4 66 LAN Configuration Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp server become the active parameters immediately In addition they are stored in the non volatile memory of the 7100 CE Thus after a power cycle they are still available This enables a kind of Bootp once configuration of the 7100 CE Example The user may not want to have a Bootp server be active in his network all the time But on the other hand he may not have any other configuration method than Bootp In this case he starts the Bootp server temporarily powers on the 7100 CE using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp server and powers down the 7100 CE Then he selects the initialization mode Using Stored and powers on the 7100 CE again From now on he is able to establish the TCP IP connection to the 7100 CE with the parameters obtained in that single Bootp cycle Bootp Active Server Non Volatile RAM Stored Parameter Parameter Figure 4 Bootp amp Store principle Use the initializati
129. es como descargas si se abre la cubierta del instrumento mientras ste permanece conectado a la corriente Aseg rese de poder acceder siempre al enchufe de corriente Retire el cable de corriente del instrumento antes de abrir la cubierta del m dulo No conecte el cable de alimentaci n al instrumento si la caja de la electr nica de la parte trasera est abierta o si se han retirado los paneles laterales ADVERTENCIA Voltaje de l nea incorrecto en el instrumento Si el instrumento se conecta a un voltaje superior al especificado existe peligro de electrocuci n o de da os en los instrumentos Conecte el instrumento al voltaje de l nea especificado 38 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 PRECAUCION Enchufe de alimentaci n inaccesible En caso de emergencia debe ser posible desconectar el instrumento de la red inmediatamente e Aseg rese de que es posible alcanzar y desconectar f cilmente el enchufe del instrumento Deje espacio suficiente por detr s de la toma de corriente del instrumento para desconectar el cable Cables de alimentaci n ADVERTENCIA El instrumento se entrega con diferentes cables de alimentaci n Los terminales hembra de todos los cables de alimentaci n son id nticos Se introducen en el conector de entrada de corriente de la parte posterior El terminal macho de cada cable de alimentaci n es dif
130. es par une personne quali fi e et consciente du danger Ne pas tenter d effectuer une op ration d entre tien ou un r glage sans la pr sence d une autre personne capable de donner les premiers secours et d assurer une r animation Ne pas remplacer les com posants quand le cable d alimentation est connect Ne pas utiliser l instrument en pr sence de gaz ou fum es inflammables Le fonctionnement de n importe quel instrument lectrique dans un tel environ nement pr sente un danger certain Ne pas effectuer des substitutions de pi ces ni des modifications non autori s es Il se peut que les condensateurs situ s l int rieur de l instrument soit encore charg s bien que l appareil ait t d branch de sa source d alimentation Des tensions dangereuses sont pr sentes dans cet instrument capables de causer des blessures graves Vous devez proc der avec extr me pr caution lorsque vous manipulez testez et ajustez cet appareil Lorsque vous manipulez des solvants respectez les r gles de s curit port de lunettes de gants et de v tements de protection d crites dans la fiche de don n es de s curit fournie par le fournisseur du solvant surtout si les solvants utilis s sont toxiques ou dangereux Agilent 7100 Capillary Electrophoresis System User Manual 239 10 Appendix Symboles de s curit Tableau 29 Symboles de s curit Symbole Description Cet appareil porte ce symbole pour indiquer
131. este manual deben hacerse con el instru mento conectado a la red y con alguna de las cubiertas de protecci n abierta El alto voltaje existente en algunos puntos puede producir da os personales si llegan a tocarse estos puntos Siempre que sea posible debe evitarse cualquier ajuste mantenimiento o reparaci n del instrumento abierto y conectado a la red Si no lo es debe rea lizarlo personal especializado consciente del riesgo existente No intentar lle var a cabo este tipo de trabajo si no est presente otra persona capaz de proporcionarle primeros auxilios en caso necesario No cambiar ning n com ponente con el cable de red conectado No ponga en marcha el instrumento en presencia de gases o vapores inflama bles El encendido de cualquier instrumento el ctrico en estas circunstancias constituye un atentado a la seguridad No instale componentes que no correspondan al instrumento ni realice modi ficaciones no autorizadas Los condensadores que contiene el aparato pueden mantener su carga aunque el equipo haya sido desconectado de la red El instrumento posee voltajes peli grosos capaces de producir dafios personales Extreme las precauciones cuando proceda al ajuste comprobaci n o manejo de este equipo Cuando se trabaje con disolventes seguir los procedimientos de seguridad apropiados guantes de seguridad gafas y ropa adecuada descritos en las especificaciones sobre el tratamiento de material y seguridad que suministra
132. f commands Displays current LAN settings ip lt X X X X gt Sets new IP address SM lt X X X X gt Sets new subnet mask gw lt X X X X gt Sets new default gateway exit Exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return Agilent 7100 Capillary Electrophoresis System User Manual 73 4 LAN Configuration 5 Use and press Enter to list the current settings F CAWINDOWSAsystemI21cmd cxe telnet 134 40 27 95 Information about the LAN interface LAN Status Page MAG Address 1 BEGEDSERON3O MAC address initialization mode Init Mode rer Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to a PC with controller software for example Agilent ChemStation here not connected Figure 11 Telnet current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list the current settings If the initialization mode switch is changed now to Using Stored mode the instrument will take over the stored settings when the instrument is re booted In the example above it would be 134 40 27 99 74 Agilent 7100 Capillary Electrophore
133. ffline copies 2 Turn off the line power to the Agilent CE instrument Open the top cover 4 Open the detector cover Agilent 7100 Capillary Electrophoresis System User Manual 225 8 Maintenance Changing the Lamp 5 Disconnect the lamp cable s 8 fold connector Pull the connector not the cable WARNING Injuries due to burns Touching a hot lamp can cause serious burns fyou were using the instrument shortly before the lamp may be very hot Wait until it has cooled down before touching the lamp Lamp screws Positioning pin Connector Figure 72 Exchanging a lamp 6 Loosen the two lamp screws using the Pozidriv screwdriver and lift the lamp out carefully 226 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Inserting a New Lamp 1 Hold the new lamp by the metal ring to avoid touching the glass surface Fingerprints absorb light and can reduce light intensity Insert the lamp into the lamp compartment Position the lamp so that the notch in the lamp ring matches with the pin on the detector housing 3 Tighten the two lamp screws f o N c Connect the lamp cable connector and fix the cables into the channel in the foam Close the detector lid without clamping the lamp cable Close the top cover Turn line power on Boot the Agilent ChemStation and turn the lamp on Allow the lamp to warm up for about 1 hour Perform an Intensity Test in the De
134. g In order to condition a packed capillary an applied pressure of approximately 12 bar alone is usually insufficient to move the mobile phase rapidly through the column Therefore the applied high pressure may be assisted by the application of an electric field This augments the high pressure flow with an electroosmotic flow In the preconditioning or postconditioning section of the set up method the High pressure flush command can be used for this purpose see Figure 48 on page 155 High pressure can be applied to the capillary inlet vial which may be A set value 2 12 bar Raw external pressure up to 15 bar Allows pressurizing the inlet vial and or the outlet vial The external pressure up to 15 bar can be applied directly without reduction High pressure flush Raw Pressure o 120 7 bar 00 K 300 pA 90 w 6 Inlet Inlet Home Vial v Outlet Outlet Home Vial v Apply pressure to Inlet Apply pressure to Outlet Apply pressure to both Figure 48 High pressure flush command menu Agilent 7100 Capillary Electrophoresis System User Manual 155 5 156 Using the Agilent 7100 Capillary Electrophoresis System Other parameters which can be set in the High pressure flush command menu include Applied voltage kV current uA or power W Duration s Inlet vial and or outlet vial When applying voltage simultaneously the inlet and oulet vials must contain electrolyte in order to maintain electri
135. genschaften Umfangreiche Diagnose Fehlererkennung und anzeige Kriechstromerkennung Grenzwert f r zu niedrigen Strom Fl ssigkeitslecksensor Sicherheitssensoren an T r und Abdeckung zur Trennung der Hochspannung Probenflaschensensor Agilent ChemStation unter Windows XP SP3 LAN CAN Controller Area Network RS 232C 0 bis 1 V APG Remote Ready Start Stop und Shut down Signale USB zuk nftige Verwendung Analoger Ein Ausgang Fr hwarnsystem f r fallige Wartungen EMF Early Maintenance Feedback elektronische Aufzeichnung von Wartungsarbeiten und Fehlermeldungen Agilent 7100 Capillary Electrophoresis System User Manual 27 2 Site Requirements and Specifications Exigences d installation Un environnement ad quat est indispensable pour obtenir des performances optimales de l instrument d lectrophor se capillaire 7100 Avant de commencer l installation v rifiez soigneusement que l emplacement choisi est conforme aux exigences ci dessous Remarques propos de l alimentation AVERTISSEMENT Le module d alimentation de l instrument pr sente une tol rance importante Il accepte ainsi n importe quelle tension de secteur se situant dans la plage de tol rance pr cis e dans le Tableau 6 page 31 Ceci explique l absence de s lecteur de tension l arri re du d tecteur Le module reste partiellement activ lorsqu il est teint tant que le cordon d alimentation est bran
136. gure 33 Installing the capillary Alternate the sides of the pins when inserting the capillary NOTE Make sure the windings of the capillary do not come into contact with each other As the ia heat cannot be conducted away at contact points they may get hot and cause separation and detection problems Such a contact point might also be a predetermined breaking point for arcing 6 Insert the other end of the capillary into the guiding hook on the right 7 Press the white capillary lock towards the center of the cassette and insert the capillary into the capillary lock Release the capillary lock to secure the capillary 8 Make sure the two capillary ends outside the cassette are the same length as the cassette guiding pins see Figure 33 on page 130 alignment position and are parallel to the pins Use a sheet of paper or another soft item with a straight line to check this Use the capillary lock to make adjustments on the inlet side 130 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Closing the Capillary Cassette 1 Place the top of the cassette onto the bottom part with an offset of 8 mm 0 3 inch 2 Hold the bottom part and slide the top part towards the top of the cassette until it clicks into place 3 Make sure the cassette cover and body form one unit 4 Make sure the two capillary ends outside the cassette are both the same length as the cassette guiding
137. he sample has been injected those two vials are positioned at the two electrodes 5 When using the replenishment system you can start off with empty buffer vials and use the replenishment system to fill them automatically with buffer Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Removing the Capillary Cassette from the Agilent CE Instrument Your Agilent CE instrument comes with an empty capillary cassette installed To remove the capillary cassette from the Agilent CE instrument you have to do the following steps 1 Choose the cassette icon in the CE diagram and select Change Cassette from the menu This removes the buffer vials from the ends of the capillary abd places them back to their position in the tray This will take approximately 5 seconds 2 Open the top cover to access the cassette Press a spring located in the middle of the front lid to release the latch Figure 26 Position of installed cassette on page 122 shows the position of an installed cassette If you do not lower the vials from the loaded position buffer which may be transferred via the capillary into the electrode inserts precipitates and may block the electrodes or damage the capillary 3 Move the cassette guide to the front Agilent 7100 Capillary Electrophoresis System User Manual 121 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis C
138. her network device will have the same hardware address The MAC address is physically located on the electronic main board in the rear box of the instrument It is not accessible by the user The MAC can be reviewed from a connected instrument by accessing it via a Telnet session as described earlier Please follow the instructions on Manual Configuration Using Telnet With Telnet on page 72 and see Figure 11 on page 74 for the location of the MAC address as the second line in the LAN status page Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System User Manual 5 Using the Agilent 7100 Capillary Electrophoresis System System Definition 78 Controlling the Agilent CE Instrument 79 Working with the CE Diagram 84 How to Prepare your Agilent CE Instrument for Analysis 91 Capillary Electrophoresis CE 92 Capillary Electrophoresis Plus High Pressure CE p 136 Capillary Electrochromatography CEC 148 This chapter provides information on how to set up the CE instrument for an analysis and explains the basic settings RE Agilent Technologies 77 5 Using the Agilent 7100 Capillary Electrophoresis System System Definition After your Agilent 7100 Capillary Electrophoresis System has been properly installed see Installing the Instrument on page 53 you can begin exploring the system Your Agilent 7100 Capillary Electrophoresis System comprises the CE instrument the ChemSta
139. ht autorisierten Ver nderungen am Ger t vor Kondensatoren in diesem Ger t k nnen noch geladen sein obwohl das Ger t von der Netzversorgung getrennt worden ist In diesem Ger t treten gef hr liche Spannungen auf die zu ernsthaften Personensch den f hren k nnen Die Handhabung berpr fung und Einstellung des Ger tes ist mit u erster Vorsicht auszuf hren Beachten Sie bitte beim Arbeiten mit L sungsmitteln die geltenden Sicher heitsvorschriften z B Tragen von Schutzbrille Arbeitshandschuhen und Sicherheitskleidung wie sie in den Sicherheitsdatenbl ttern des Herstellers beschrieben sind dies gilt speziell f r der Handhabung giftiger oder gesund heitsgef hrdender L sungsmittel Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Sicherheitssymbole Tabelle 28 Sicherheitssymbole Symbol Beschreibung gt QE bB Das Ger t ist mit diesem Symbol markiert wenn der Benutzer im Handbuch nachlesen sollte um sich vor Verletzungen und das Ger t vor Besch digungen zu sch tzen Weist auf gef hrliche Spannungen hin Weist auf einen Schutzkontakt Erdung hin Das Licht der Xenon Lampe in diesem Produkt kann bei direktem Blickkontakt zu Augenverletzungen f hren Das Ger t ist mit diesem Symbol versehen wenn hei e Oberfl chen vorhanden sind mit denen der Benutzer nicht in Ber hrung kommen sollte Der Sicherheitshinweis WARNUNG weist Sie auf Situationen hin die zu
140. i alimentazione diversi L estremit femmina sempre uguale e deve essere introdotta nell apposita presa di alimentazione che si trova nella parte posteriore L estremit maschio di ciascun cavo di alimentazione diversa ed progettata per adattarsi alle prese utilizzate nei vari paesi Assenza di messa a terra o uso di un cavo di alimentazione non specificato L assenza del collegamento a terra o l uso di un cavo di alimentazione non specificato pu provocare scosse elettriche o cortocircuiti Non utilizzare mai lo strumento con prese prive di messa a terra Non utilizzare cavi di alimentazione diversi da quelli predisposti da Agilent Technologies per i singoli paesi ATTENZIONE Uso di cavi non forniti L uso di cavi non forniti da Agilent Technologies pu provocare danni ai componenti elettronici o lesioni personali Utilizzare solo cavi forniti da Agilent Technologies in modo da assicurare il funzionamento corretto e la conformit alle norme di sicurezza o alle normative EMC 34 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Spazio necessario Le dimensioni e il peso dello strumento ne consentono il posizionamento sulla maggior parte dei banchi o tavoli di laboratorio necessario lasciare un ulteriore spazio di 2 5 cm su entrambi i lati e di circa 8 cm nella parte posteriore per la circolazione dell aria Se si posiziona sul banco l intero Sistema
141. ials for Maintenance Overview of Maintenance Parts Table 26 Maintenance parts Description Part Number Long life Deuterium lamp 8 pin Long life HiSensitivity Deuterium lamp 8 pin Electrode assembly standard Electrode assembly short Electrode O ring silicone 5 pk Electrolyte bottle 500 mL Electrolyte bottle 100 mL Electrolyte bottle cap Bottle sealing O ring Glass filter solvent inlet Filter frit adapter 3 mm 4 pk Pre puncher Screws for pre puncher 10 pk Air filter 5 hm Capillary cassette Optical filter for DAD 260 nm 5190 0917 5190 0916 G7100 60007 G7100 60033 5062 8544 9300 1748 5042 6478 9300 1747 0905 1163 5041 2168 5062 8517 G1600 67201 G1600 62402 3150 0619 G7100 60002 G7100 62700 Vials See Table 23 Alignment interfaces See Table 24 Fuses See 7100 Capillary Electrophoresis Instrument Electronics on page 173 230 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System User Manual 10 Appendix General Safety Information 232 Allgemeine Sicherheitsinformation 235 Informations g n rales de s curit 238 Informazioni generali sulla sicurezza 241 Informaci n de seguridad 244 222999299299 247 250 Setting Up a Test Sample Run 252 Chemical and Biological Safety 256 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 257 Radio Interference 258 Sound Emission 259 Legal Noti
142. ilent CE instrument for the analysis you need to do the following tasks Start the Agilent CE instrument e Start the computer Start the Agilent ChemStation software CE user interface Allow the lamp to warm up approximately 1 hour Prepare vials for sample and buffer Insert a capillary into the alignment interface Insert the capillary into the capillary cassette Insert the capillary cassette into the system Prepare the replenishment system optional After you have completed all these tasks you can start an Agilent CE method A CE method can be created in an online CE instrument is on and connected or offline installation of the Agilent ChemStation The detector lamp can be switched on automatically and unattended by scheduling this from the detector control menu Please see the options for the detector under Diode Array Detector on page 179 The detector lamp can be switched on automatically with each instrument start Select the respective setting from the detector control menu see Diode Array Detector on page 179 Agilent 7100 Capillary Electrophoresis System User Manual 91 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis CE 92 How to Use Your Agilent CE Instrument for Capillary Electrophoresis Separations This chapter introduces method parameters for applications running in CE mode This mode is the most suitable for applications in the field of
143. ilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 7100 CE instruments This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their CE instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual CE instrument The tests and diagnostic features provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files Configuration The Lab Advisor software can communicate with a 7100 CE instrument via LAN or by a serial connection RS232 cable null modem To configure a connection go to the section Configuration in the Lab Advisor software Select Instrument gt Available Instruments and as the instrument type Agilent CE You can enter either the LAN address for example 192 168 254 11 or the respective COM port of your PC for example Com1 under instrument address Agilent 7100 Capillary Electrophoresis System User Manual 169 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 170 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System User Manual 7 Hardware Info
144. in the Lamps dialog Changes are applied only after clicking 0K A checkmark in the section At Power On allows you to immediately turn on the deuterium lamp when the instrument is switched on In the Automatic Turn On field you can also set a date and time when the lamps are switched on automatically The instrument must be kept powered on for example overnight to use this feature 180 Agilent 7100 Capillary Electrophoresis System User Manual Hardware Information 7 Digital to Analog Converter The 7100 CE System provides a direct analog output of chromatographic signals A It may be used to connect to another data acquisition system or chart recorders The analog signal is converted from digital format by the on board 20 bit digital analog converter DAC Please select the suitable voltage range for your analog recording hardware under Analog Output Range Agilent 7100 Capillary Electrophoresis System User Manual 181 7 Hardware Information Changing the Fuses for the Power Supplies Prior to changing fuses you must switch the instrument off and disconnect it from the line power Fuses are located on the back right side of the instrument Replace fuses with identical part numbers as specified under 7100 Capillary Electrophoresis Instrument Electronics on page 173 KIT e Using wrong fuses can result in fire hazard For continued protection against fire hazard replace fuses only with the same type and ratings
145. in the WEEE Directive Annex I this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Agilent 7100 Capillary Electrophoresis System User Manual 257 10 Appendix Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 258 Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound Pressure Lp 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test Agilent 7100 Capillary Electrophoresis System User Manual 259 10 Appendix Legal Notice The Agilent 7100 Capillary Electrophoresis System is sold for research only By purchasing this instrument the purchaser
146. int free cloth Optical alignment interface Inspect for occlusion under microscope or magnifier dust or shards of polyimide If capillary was broken while in the interface inspect and remove fragments ultrasonic device and air burst Clean for storage with isopropanol water Filter all solutions prior to use 0 2 or 0 45 um ifthe environment is very dusty ifthe system cannot generate enough pressure atleast every three months Use the red labeled alignment interface without capillary for tests Agilent 7100 Capillary Electrophoresis System User Manual 191 8 Maintenance Table 25 Overview of maintenance Procedure Typical frequency Notes Repair of replenishment needle Buffer reservoir Inlet frit Vial caps Use the Clean Level Sensor task to remove salt deposits inside the tubing system Keep a water filled parking vial in carousel position 49 Occasionally replace the parking vial in position 49 with water or buffer Inspect for bending if clogged Remove persistent clogging by a syringe flush with water isopropanol or use ultrasonic device Inspect O ring in cap for damage remove salt deposits Do not use buffer reservoir for long term storage prevents oxygen saturation and bacteria from growing Remove liquid from waste bottle Inspect and clean especially if absorbing buffers have been used Replace when dirty Do not overuse vial caps especially the PEO
147. ion plate 3 Use the 12 mm hexagonal socket screwdriver to unscrew the inlet electrode 12 mm hexagonal socket screwdriver High voltage cable red PN 35 SEN Ground cable blue Figure 59 Removing the ground cable and the inlet electrode cable Agilent 7100 Capillary Electrophoresis System User Manual 199 8 Maintenance Cleaning the Electrodes Pre punchers and Insulation Plate 4 Carefully lift the red high voltage wire The inlet electrode will come out 5 Slowly slide the insulation plate first to the right 1 then tilt it up 2 and 3 and finally take it out Make sure that the outlet electrode is not touching the foam of the tray cooling or the lift station 1 Right shift 2 Rotation 3 Take out Figure 60 Removing the insulation plate NOTE It is not possible to remove the outlet electrode without taking out the insulation plate 6 Remove the outlet electrode from the insulation plate using the 12 mm hexagonal socket screwdriver 200 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Accessing the Liquid Handling Module CAUTION Damage to tubes and liquid spills Keeping tubes connected on the left side of the instrument while pulling out the liquid handling module will damage the tubes and might lead to liquid spills Disconnect any external pressure supply drainage or connections to an external water bath for tray cooling prior to accessing the liquid handling module
148. ique Commentaires Fonctions de s curit Commande et traitement des donn es Communication Caract ristiques BPL Diagnostic tendu d tection et affichage des erreurs d tection du courant de fuite limite de courant faible D tecteur de fuites capteurs de s curit sur la porte et le couvercle avec fonction de d sactivation de haute tension capteur de flacons Agilent ChemStation sous Windows XP SP3 R seau local bus CAN RS 232C commande 0a1V distance CAG signaux ready start stop et shut down USB utilisation ult rieure Sortie et entr e analogiques Maintenance pr dictive EMF enregistrement lectronique des op rations de maintenance et des erreurs 32 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Requisiti ambientali Un ambiente adatto importante per garantire le prestazioni ottimali del Sistema per elettroforesi capillare Agilent 7100 Prima di effettuare l installazione controllare attentamente che il luogo scelto per l installazione soddisfi i requisiti indicati di seguito Alimentazione ATTENZIONE Lo strumento pu essere utilizzato in un ampio intervallo di valori di tensione ed in grado di accettare tensioni di linea compresi negli intervalli indicati nella Tavola 8 a pagina 36 Pertanto nella parte posteriore del rivelatore non presente alcun selettore di frequenza Quando spento se il c
149. is granted the limited right to use only this instrument Purchase of this instrument does not include any right express or implied to use any other patented product method or process or to use any other portion or component of any patented system or systems software microfluidic devices or reagents either alone or conjunction with this product unless use of such method process or other portion or component is separately authorized 260 Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 7100 Capillary Electrophoresis System for download Agilent 7100 Capillary Electrophoresis System User Manual 261 10 Appendix Agilent Technologies on Internet 262 Agilent 7100 Capillary Electrophoresis System User Manual Index A accessing 199 accessories 55 230 acquisition current 105 detector signals 107 power 99 pressure 105 spectra 108 temperature 97 voltage 98 AD converter 181 186 alignment interface 124 191 color coding 124 installation of capillary 124 removing 121 132 autobalance 109 buffer 111 buffer vials 115 change 111 change during run 111 composition 217 liquid level 117 preparation 111 replenishment 111 C capillary installation into cassette 12
150. ission 259 Legal Notice 260 Agilent Technologies on Internet 261 Index 263 Agilent 7100 Capillary Electrophoresis System User Manual 11 Contents 12 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System M User Manual e09 e 1 270 Introduction x Introduction to the Agilent 7100 Capillary Electrophoresis System 14 Where to Find Information 15 What Learning Products are Available 16 This chapter gives an introduction to the Agilent 7100 Capillary Electrophoresis System and where to find specific information or help RE Agilent Technologies 13 1 Introduction Introduction to the Agilent 7100 Capillary Electrophoresis System This handbook gives an overview of your Agilent 7100 Capillary Electrophoresis System It is designed to get you started working with the system We strongly advise you to make extensive use of the online help when working with the system There you find detailed reference and task information which complements the overview given in this guide Refer to Getting Help later in this section for information on using the online help of the ChemStation Refer to Where to Find Information for help in finding information on certain tasks Getting Help You can get help by choosing the Help item from the top menu This puts you into the help index From there you can browse through the information by selecting the appropriate jump
151. ith a high viscosity a larger pore size for example 0 45 um is also suitable 2 Fill the electrolyte bottle with buffer For 500 mL bottles part number 9300 1748 use a minimum of 100 mL to make sure the frit is submerged in buffer Use a maximum of 400 mL For 100 mL bottles part number 5042 6478 use a minimum of 40 mL to make sure the frit is submerged in buffer Use a maximum of 80 mL 3 Tighten the cap Make sure the cap is tightened firmly as pressure will be applied to the bottle 4 Choose OK in the Change Bottles dialog box Wait until the pressure has built up again that is the instrument reaches the ready state The right hand bottle is the waste bottle It is used to store buffer emptied from vials in the tray Empty the bottle whenever you refill the electrolyte bottle following the same procedure as described above Empty the bottle frequently The error message Waste Bottle Full appears if the waste bottle is full Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Preparing Vials for Buffer and Sample Vial Handling Only use only recommended vials see Table 4 when working with the Agilent CE instrument Itis recommended cleaning the vials prior use flush them with water and some alcohol use compressed air to dry them otherwise particles may enter the capillary current problems or blocked capillary All vials must be capped
152. ity detector This option allows you to save lamp life time Detector Timetable Using the Timetable for the detector you can program the time for the following parameters during your analysis Measuring wavelength for signals A to H Measuring bandwidth for signals A to H Reference wavelength for signals A to H Reference bandwidth for signals A to H Spectra acquisition mode Peak width Threshold By programming the time for these parameters you can optimize the required disk space for your spectra by switching the spectra acquisition on and off Thus you can acquire spectra for parts of the electropherogram only Agilent 7100 Capillary Electrophoresis System User Manual 109 5 Using the Agilent 7100 Capillary Electrophoresis System Switching the Lamp On The lamp can be switched on automatically as part of initialization see DAD Control on page 180 To switch the lamp on or off manually 1 Inthe DAD diagram click the lamp icon A menu will offer the option to switch the lamp on or off depending of the current status Please see Figure 23 for the different lamps statuses in the DAD diagram Off Ignition Figure 23 UV lamp status Off Ignition On 2 To ignite the lamp from the menu go to Instrument gt More DAD gt UV Lamp and select Switch Lamp On or Switch Lamp Off Please see Figure 23 for the different lamps statuses in the DAD diagram 3 Prior to a run allow the lamp to warm up for a
153. lary This injection technique is often used for capillaries filled with fixed or cross linked gels or other high viscosity material where injection by pressure is not applicable During electrokinetic injection molecules with different mobilities will be depleted differently from the sample solution therefore gradual changes in analyte composition might occur as a consequence of multiple injections Different salt contents in the sample matrix will influence the injection efficiency of the analytes Variation in the matrix composition might therefore bias the quantitative analysis and in the worst case hamper analyte introduction Difference Between Hydrodynamic and Electrokinetic Injection To do electrokinetic injection the electrode must touch the sample in the sample vial In contrast with hydrodynamic injection the sample only needs to touch the capillary tips Short electrodes G7100 60033 in combination with hydrodynamic injection are used to minimize sample carry over to other vials At appropriate low fill volumes present in the sample vial during injection electrodes are not submerged With this procedure there is no interaction between sample and electrode such as electrochemical reactions or adhesion of sample to the electrode This precaution can be programmed into the timetable by a combination of the commands load vial and wait 104 Agilent 7100 Capillary Electrophoresis System User Manual Using th
154. lease be aware that most coated capillaries will be irreversibly damaged by this To minimize contamination of buffer vials with conditioning solutions such as NaOH which might stick to the outside of the capillary or electrode it is recommended to dip the electrode in an additional step in a large dedicated vial filled with buffer prior to letting the instrument load the actual inlet and outlet home vials with run buffer This precaution can be programmed into the timetable by a combination of the commands load vial and wait Agilent 7100 Capillary Electrophoresis System User Manual 101 5 102 Using the Agilent 7100 Capillary Electrophoresis System Wash Inlet Electrode New Command to Avoid Carry over in Critical Applications During precondition and postconditioning a command for emptying the space between capillary and electrode can be executed The command is dedicated for applications with aqueous buffers and consists of application of slight vacuum to the inlet vial while lowering the inlet vial This leads to opening of an internal sealing of the electrode and an airstream inside the wash vial at the inlet lift Liquid stored in the space between capillary and electrode is removed It is recommended to perform this prior to a flush step with run buffer in case of carry over critical applications The wash inlet electrode command is not available for the outlet electrode At the inlet lift a dedicated wash vial should be presen
155. m internal diameter Use those fittings for the flexible tubing used with your external water bath Connect the fittings to the connectors on the instrument see Figure 2 on page 59 Use the upper connector for incoming liquid the lower connector for outgoing liquid Make sure both the water bath and the Agilent CE instrument are switched off when installing the external water bath Risk of liquid spill and instrument damage Higher pressure might lead to liquid leakage inside and outside the instrument Elevated temperatures might lead to deformation and leakage of the tubing system Liquid spillage can damage electronic components of the instrument Do not use a water bath with more than 0 5 bar pressure Do not use liquids at temperatures above 50 C Agilent 7100 Capillary Electrophoresis System User Manual 185 7 Hardware Information Analog to Digital Converter Some detectors for example CCD detectors require an intermediate interface to convert analog data into a digital format that can be sent to the ChemStation Analog In The Agilent 7100 Capillary Electrophoresis System has a 20 bit AD converter built in It enables voltage conversions ranging from 0 V to 1 V You can set up name units and conversion factors for the analog in signal from the following dialog which is accessible under Instrument configuration CE part configuration see Figure 56 186 Agilent 7100 Capillary Electrophoresis System User Manual
156. n Sie sich dass nur Sicherungen mit dem korrekten Nennstrom and dem richtigen Typ normale Schmelzsicherung tr ge Sicherungen usw verwendet werden Die Benutzung reparierter Sicherungen sowie das Kurz schlie en von Sicherungshaltern sind nicht zul ssig Agilent 7100 Capillary Electrophoresis System User Manual 235 10 Appendix 236 Einige in diesem Handbuch beschriebenen Einstellarbeiten werden bei an das Stromnetz angeschlossenem Ger t und abgenommener Geh useabdeckung durchgef hrt Dabei liegen im Ger t an vielen Punkten hohe Spannungen an die im Falle eines Kontaktschlusses zu Personensch den f hren k nnen S mtliche Einstellungs Wartungs und Reparaturarbeiten am ge ffneten Ger t sollte nach M glichkeit nur durchgef hrt werden wenn das Ger t von der Netzspannung getrennt ist Solche Arbeiten d rfen nur von erfahrenem Personal durchgef hrt werden das ber die Gefahren ausreichend informiert ist Wartungs und Einstellarbeiten an internen Ger tekomponenten sollten nur im Beisein einer zweiten Person durchgef hrt werden die im Notfall Erste Hilfe leisten kann Tauschen Sie keine Komponenten aus solange das Netzka bel am Ger t angeschlossen ist Das Ger t darf nicht in Gegenwart brennbarer Gase oder D mpfe betrieben werden Ein Betrieb von elektrischen Ger ten unter diesen Bedingungen stellt immer eine eindeutige Gef hrdung der Sicherheit dar Bauen Sie keine Austauschteile ein und nehmen Sie keine nic
157. n check if the electrode exit points fit exactly to the pre puncher funnel exit The electrode or needle might need careful manual bending to make it point towards the exect center of the pre puncher funnel During the alignment check check button the electrode or replenishment needle passes through the funnel several times An improperly aligned electrode slides along the pre puncher funnel The orientation is optimum if the electrode does not move sidewards during this dynamic check Although electrodes will most likely not need alignment thanks to their design you should still perform the check alignment procedure The electrodes are fragile Manual bending during alignment should be done only with great care Electrodes must be kept straight Avoid curving Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Reinstall the Capillary Cassette See Inserting the Cassette on page 132 Reinstall the Front Cover 1 Make sure the front cover screws have a horizontal orientation They can only click into place in this orientation 2 Keep the front door of the cabinet slightly open 3 Start mounting the front cover by inserting it at the lower end first 4 Carefully push the front cover into the main frame You might need to pay special care on the right side due to a connector 5 Close the lid of the instrument Agilent 7100 Capillary Electrophoresis System User Manual 215 8 Maintenance Maintenance
158. n conditioning are not replenished at the same time otherwise you will have a vial conflict Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 The time for a replenishment step can vary from vial to vial When using buffers containing detergent for example SDS replenishment may not fulfill your needs The limit for SDS concentration is 70 mM Do not exceed this concentration Add some organic liquid to the waste bottle so that bubbles do not form Also see Problems with the Replenishment System and Buffers Containing SDS on page 168 Preconditioning In order to have stable conditions for your analysis it is important to condition capillaries Depending on the separation mode used it can comprise conditioning the capillary with conditioning agents and equilibrating the capillary with buffer These conditioning steps can be programmed using an expandable table in the right side of the CE Method Setup screen The associated timetable can be used to apply pressure apply voltage or flush between vials Inlet and outlet vials can be loaded and wait time can be programmed here If suitable such steps can also be done as a postconditioning operation Preconditioning and Postconditioning on page 143 Condition capillaries according to their specifications Fused silica capillaries are typically rinsed with alkaline or acidic solutions for conditioning P
159. n de este instrumento Si no se cumplen estas normas o los avisos espec ficos que aparecen en diversas partes de este manual se invalidan los est ndares de seguridad de disefio fabricaci n y utili zaci n de este instrumento Agilent Technologies no se responsabiliza del incumplimiento de estos requisitos por parte del usuario ste es un instrumento de seguridad de Primera Clase dotado de un terminal de toma de tierra y ha sido fabricado y comprobado de acuerdo con las nor mas internacionales de seguridad Operaci n 244 Antes de conectar el instrumento a la red siga atentamente las instrucciones de la secci n de instalaci n Adem s debe tener en cuenta lo siguiente No retire las cubiertas del instrumento mientras est funcionando Antes de conectar el instrumento todos los cables de tierra alargadores transformado res y aparatos conectados al mismo deben conectarse a tierra mediante un enchufe adecuado Si se interrumpe la conexi n a tierra pueden producirse dafios personales serios Siempre que se sospeche que la conexi n a tierra se ha interrumpido debe dejarse el aparato inoperativo y evitar cualquier mani pulaci n Compruebe que se utilizan los fusibles de recambio adecuados y del tipo espe cificado Deben evitarse la utilizaci n de fusibles reparados y los cortocircui tos en los portafusibles Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Algunos de los ajustes descritos en
160. n the background It tests the wavelength calibration of your detector and advises you to make adjustments if necessary If the lamp is off or the cassette is not inserted properly the wavelength cannot be adjusted correctly 8 Close the Change Cassette dialog box by choosing 0K enter the capillary information and proceed to setting up a method One of the following not ready conditions may appear in the status display of the Agilent ChemStation Move the mouse over the status display to read the not ready conditions Recalibration necessary UV lamp not ready To correct for these not ready conditions please reinsert the cassette Make sure the alignment interface snaps onto the ceramic interface of the detector and is not occluded Make sure the lamp is ignited Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Fraction Collection In the bioscience and pharmaceutical sectors the capability of fraction collection is frequently required in order to characterize separated components by off line analysis techniques such as MALDI TOF MS or protein sequencing All 7100 CE instruments allow fraction collection This feature will be fully available in future Agilent ChemStation software revisions Please check regularly for updates at http www agilent com in the internet Agilent 7100 Capillary Electrophoresis System User Manual 135 5 Using the Agilent 7100 Ca
161. ntinfortunistici come descritto nella documentazione fornita con il materiale specialmente in presenza di solventi tossici o pericolosi Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Simboli di sicurezza Tabella 30 Simboli di sicurezza Simbolo Descrizione Questo simbolo segnala all utente che necessario consultare il manuale per l uso per prevenire lesioni personali o danni alle apparecchiature Indica la presenza di tensioni pericolose Indica un terminale di messa a terra Indica il rischio di lesioni agli occhi in caso di esposizione diretta alla luce prodotta dalla lampada al deuterio inclusa nel prodotto Questo simbolo indica la presenza di superfici surriscaldate che non devono essere toccate dall utente ATTENZIONE L indicazione ATTENZIONE segnala situazioni che possono provocare lesioni fisiche o mortali e Prima di continuare a usare lo strumento verificare di aver compreso e attuato quanto indicato nell indicazione di attenzione AVVERTENZA ILE L indicazione AVVERTENZA indica situazioni che possono causare una perdita di dati o danni allo strumento Non procedere oltre finch non stato compreso ed eseguito quanto indicato Agilent 7100 Capillary Electrophoresis System User Manual 243 10 Appendix Informaci n de seguridad General Las siguientes precauciones generales deben aplicarse durante el funciona miento mantenimiento o reparaci
162. of the Replenishment System The replenishment system automatically empties and refills the buffer vials in the tray with new buffer from the electrolyte bottle Typically replenishment is done before every run or after every 3 to 4 runs depending on the stability and volume of the running buffer Initial preparation of the replenishment system is described in Preparing the Replenishment System if Needed on page 111 Please see the general instructions on operations for replenishment maintenance such as flush tubes clean tubes and clean level sensor Operations for Replenishment Maintenance on page 219 Frequency The replenishment system bottles and tubing should be cleaned if using it for the first time changing the buffer or the replenishment system will be idle for some time Requirements Vials and caps Water and or buffer and isopropanol depending on the protocol selected ChemStation Lab Advisor or Instrument Utilities software 216 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Changing the Buffer Composition in the Replenishment System Please follow these steps 1 Open the Lab Advisor or Instrument Utilities software and start monitoring the respective instrument In the Tools section select the CE Control screen and click on Change Bottles to release the pressure before you open the bottles Take out the electrolyte bottle and unscrew the cap The el
163. om alignment interfaces for earlier instruments Interfaces from earlier instruments do not fit into the diode array detector block Color Coding of Capillaries and Alignment Interfaces The alignment interface must correspond to the type of capillary used There are special alignment interfaces for straight capillaries and for extended light path capillaries The alignment interface must also correspond to the inner diameter of the capillary To make it easier to match the capillary and corresponding alignment interface we use the following color coding on both the sleeve of the alignment interface and the plastic stop on the capillary 124 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Table 24 Alignment interfaces Sleeve Color Part Number Used For Black G7100 60150 25 nm id capillaries with extended light path Green G7100 60210 50 um id straight capillaries Red G7100 60230 50 um id capillaries with extended light path Blue G7100 60310 75 um id straight capillaries Yellow G7100 60330 75 um id capillaries with extended light path Use the blue alignment interface for 100 or 150 nm id capillaries 1 Make sure the color of the sleeve in the alignment interface matches the color of the plastic stop on the capillary 2 Remove the protective plastic cap that covers the detection window The capillary is very fragile at that point and it is important to k
164. om its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g safety glasses gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 7100 Capillary Electrophoresis System User Manual 233 10 Appendix Safety Symbols Table 27 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death Do not proceed beyond a warning until you have fully understood and met the indicated conditions A CAUTION alerts you to situations that could cause loss of data or damage of equipment wo CAUTION Do not proceed beyond a caution until you have fully understood
165. on mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore if the 7100 CE is to obtain its parameters from a Bootp server every time it is powered on the recommended initialization mode is Bootp Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration 4 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the instrument The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Stored Parameter Active Parameter Figure 5 Using Stored principle Using Default When Using Default is selected the factory default parameters are used instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 19 on page 68 A direct connection of a computer and the 7100 instrument is the entry point to change the stored parameters of the instrument The default parameters of the 7100 instrument are permanently stored and cannot be changed Using them this allows you to establish a LAN connection at any time when the init mode Using Default is configured Default Active Parameter Parameter Figure 6 Using Default principle Using the default address in your local area network may result in network problems Take care and ch
166. on the gas supply Select under Instrument in the CE diagram the Instrument Configuration Menu see Figure 37 Instrument Configuration Configurable Modules Selected Modules Agilent CEG7100A i Agilent CE G7100A Auto em Configuration em CE Part CE Part 1671504 PP00000007 SY DAD Pat SH DAD Par 1671514 PP00000007 BinPump Generic Ask for configuration change at ChemStation startup Figure 37 Configuration menu 140 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrophoresis Plus High Pressure CE p 6 Enable CE p mode by selecting the option External Pressure Installed CE Part Configuration Instrument 1 G7100 Analog In Figure 38 Select CE mode 7 Confirm your selection by clicking OK 8 Make sure tube 8 is connected see Outlet Lift Connection to the Tubing System on page 138 9 Close and restart ChemStation to fully apply the changes Agilent 7100 Capillary Electrophoresis System User Manual 141 5 142 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis Plus High Pressure CE p 10 The activated CE p mode is displayed in the top line of the CE diagram and a new icon for an external pressure reservoir will appear see Figure 39 CHECKOUT_PHAP M Ww dl HH Ide RIN CE p Idle MN edili 1 WL B
167. onnector or fuses are located on the right hand side of the electronic box on the back of the instrument All connectors for liquids and external pressure supply are located on the left hand side of the electronic box 1 Ensure that the line power switch on the front of the instrument is OFF 2 Connect the following cables as shown in Table 2 and in Figure 2 a One power cable h LAN cable which connects the Agilent CE instrument directly to the computer with the Agilent ChemStation installed or to the local network For a description of remaining items refer to the Hardware section For more information on cable connection to 3rd party detectors or analog out please refer to Hardware Information on page 171 Agilent 7100 Capillary Electrophoresis System User Manual Installing the Instrument 3 Installing the Instrument Electrical connectors o ks 1 r OL F4 De n Do De F2 F6 T3 15AL Ft F6 250Vac Connectors for liquids and external pressure un supply LAN connector Eg TII Liquid cooling in connector Liquid cooling out connector External pressure connector Drainage Line power Figure 2 Connections on the rear of the instrument Agilent 7100 Capillary Electrophoresis System User Manual 59 3 Installing the Instrument Installing the Instrument 60 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Elect
168. or short electrodes A buffer level of 1 4 cm is recommended for standard electrodes and 1 8 mm for short electrodes You can use the replenishment system to fill the buffer vials You can also use the replenishment system to precisely lower the liquid level for all buffer vials to an equal height if you overfill them initially 118 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Outlet Vials Outlet vials for example waste vials should also be filled with enough liquid to touch the capillaries Thus the liquid flow through the capillary does not produce droplets at the capillary end which can cause pressure This would influence the injection amount If the waste vial is filled with liquid outgoing droplets cannot be drawn up the electrode by capillary effects but are flushed completely out of the outlet electrode Therefore they cannot contaminate the lift head or the next outlet vial Outlet vials that are used during analysis for example for fraction collection need to be filled to a higher level 1 cm The liquid must touch the electrode to enable a current to flow Vial in Position 49 Replenishment Lift Using replenishment it is good practice to have a capped vial filled with buffer or water in position 49 home position of the replenishment lift The replenishment needle stays in the liquid and is prevented from buffer precipitation and the resulting blo
169. or this technique is 5 mm for standard and short electrodes The short electrode filling height should not exceed 6 mm This electrode is designed to minimize take over of sample that absorbs to or simply coats the electrode Electrokinetic Injection When using injection by voltage or current the sample level needs to touch the electrodes Therefore the minimum liquid level in the sample vial is 10 mm Short electrodes G7100 60033 are not suitable for electrokinetic injection The effective length in capillary electrophoresis defined by the distance from the injection end to the detector generally ranges from 40 to 75 cm Much shorter effective lengths such as 8 5 cm from outlet side to the detector are also sometimes very useful Short effective lengths have the advantage of reduced analysis times as well as improved peak efficiency and sensitivity Hydrodynamic or electrokinetic injection from the short end of the capillary can be achieved by placing the sample vial at the outlet the buffer vial at the inlet and then applying a vacuum or a respective electrical field With the Agilent CE system up to 100 mbar pressure or vacuum can be applied to the inlet to inject sample at the inlet and outlet respectively Buffer Vials When the run is started the liquid level in the vial should touch the electrodes to enable a current to flow Therefore the minimum liquid level in the buffer vials is 1 cm for standard electrodes and 1 5 cm f
170. or to switch off automatically if liquid is lost Do not operate the devices at temperatures below 1 C to avoid formation of ice on internal surfaces External Water Bath Requirements Minimum cooling power 100 W at bath temperatures of 5 C e Temperature precision better than 0 2 C Safety requirements must meet national standards and also be suitable for unattended operation be suitable for continuous operation and have controllable high temperature protection Requirements for external liquid circulation Suitable for external circulating of liquid Suitable for connection of 8 mm ID tubes Minimum circulating liquid flow rate 8 L min 184 Agilent 7100 Capillary Electrophoresis System User Manual CAUTION Hardware Information 7 Minimum differential pressure of 150 mbar and Maximum pressure of 500 mbar Requirements for pressure pump A pump with the capacity to generate a differential pressure of at least 0 15 bar For a single action pump a high pressure capability of 0 15 bar is required The high pressure must not exceed 0 5 bar and the low pressure must not exceed 0 5 bar Cooling liquid Distilled water plus a maximum 3 anti freeze Bath temperature The minimum allowed temperature is 1 C and the Maximum allowed temperature is 50 C Connecting an External Water Bath The startup kit supplied with the Agilent CE instrument includes two fittings for flexible tubing with 8 m
171. oteins A linear voltage ramp from 0 to 30 kV can be programed by starting with 0 kV and setting the voltage in the timetable to for example 0 2 minute Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Voltage Current and Power Voltage current and power are related to each other by the resistance of your capillary buffer system You can set values for each of these three parameters The individual values are treated as limits The limit that is reached first applies You can only enter absolute values for current and power If you only want to control the voltage and not the current and power you can set those two to the system limit typically 300 uA 6 W and the system will permit the maximum value Lower Alarm Limit for Current When using constant voltage mode the current is normally the indicator of the stability of the system A rapid current decrease or drop can indicate instability in the system By setting a lower alarm limit you can define when you want to be warned of such instable conditions during the run Instable conditions can be caused by No buffer in the capillary Air bubble in the capillary Clogged capillary Broken capillary Current values are dependent on the buffer concentration the type of buffer used the inner diameter and length of the capillary and the temperature When running the Example Method for the Test Sample see
172. ould result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected fr
173. ous les c bles Elle s enfiche dans l embase d alimentation situ e a l arriere L extr mit m le qui se branche sur la prise de courant murale varie selon le pays ou la r gion Absence de raccordement la terre et utilisation d un c ble d alimentation non adapt L absence de raccordement la terre et l utilisation d un c ble d alimentation non adapt peuvent entrainer une lectrocution ou un court circuit N utilisez jamais une prise de courant sans mise la terre N utilisez jamais de cable d alimentation autre que le mod le Agilent Technologies destin votre pays AVERTISSEMENT Utilisation de cables non fournis L utilisation de cables non fournis par Agilent Technologies pourrait endommager les composants lectroniques ou provoquer des blessures Pour garantir un bon fonctionnement et le respect des r gles de s curit ou de compatibilit lectromagn tique n utilisez jamais d autres c bles que ceux fournis par Agilent Technologies Agilent 7100 Capillary Electrophoresis System User Manual 29 2 Site Requirements and Specifications Encombrement Les dimensions et le poids de l instrument permettent un placement sur quasiment n importe quel bureau ou paillasse de laboratoire Veillez pr voir un d gagement de 2 5 cm de part et d autre et d environ 8 cm l arri re pour assurer la circulation de l air et le passage des raccordements lectriques Si le syst
174. ovides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The CE instrument provides EMF counters for the CE part and the detector The counters increment with instrument use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded The counters can be reset to zero after the related parts are exchanged The CE instrument provides the following EMF counters Analysis start counter Accumulated air pump on time e Accumulated UV lamp on time Number of ignitions Using the EMF Counters The user settable EMF limits for the EMF counters enable the Early Maintenance Feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analysis high or low sensitivity analysis wavelength etc therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Setting the EMF Limits The setting for the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the instrument counters Enter these values or v
175. ower cord from the Agilent CE instrument 7 Disconnect any of the following connection if present waterbath external pressure supply and drainage KT UU If you were using the Agilent CE instrument shortly before the lamp may be very hot Avoid touching the lamp Wear protective gloves Agilent 7100 Capillary Electrophoresis System User Manual 197 8 Maintenance Cleaning the Electrodes Pre punchers and Insulation Plate Remove the Covers 1 Open the top cover 2 Remove the capillary cassette see Removing the Capillary Cassette from the Agilent CE Instrument on page 121 3 Turn the front cover screws by a quarter of a turn to vertical orientation They are not meant to be removed completely 4 Open the door to the cabinet a little 5 Carefully pull the front cover away from the main frame Pull steadily on both sides You will need slightly more force to remove the right side due to a connector Turn the screws back to horizontal orientation Screws Front cover Connector Figure 57 Removing the front cover 198 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 Cleaning the Electrodes Pre punchers and Insulation Plate Accessing the Electrodes 1 Use a screwdriver to unscrew the two metal screws holding the insulation plate 2 Use a screwdriver to remove the screw that secures the blue ground cable ME Metal screws Insulation plate Figure 58 Insulat
176. pillary Electrophoresis System Capillary Electrophoresis Plus High Pressure CE p The Agilent CE instrument is capable of accepting an external gas pressure in addition to the internal pressure The external high pressure source can be up to 15 bar 218 psi and is regulated within the instrument via method settings or with direct instructions from the CE diagram between 2 12 bar This pressure can be applied to one or both vials at the capillary ends The high pressure option may be required if using highly viscous replaceable gels in the CGE Requirements External pressure supply lt 15 bar of oil free air or nitrogen Push fit connector and gas line supplied in startup kit Outlet lift connection to the tubing system For external pressure supply a male adapter fitting the instrument s female adapter and PTFE tubing 1 8 OD x 1 16 ID PTFE tubing are part of the accessory kit of the instrument Please add a suitable connector to the external pressure supply The inlet for the external high pressure gas is situated at the back of the instrument 136 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis Plus High Pressure CE p In connector Out connector External pressure connector Drainage Figure 35 External high pressure gas inlet Agilent 7100 Capillary Electrophoresis System User Manual 5
177. power Open the front cover by releasing two screws and pulling it towards you Loose two screws to release the liquid handling module Pull the liquid handling module towards you Remove the plastic screw of the outlet lifter left eo M 5 c Ft ww MM Extract the pre puncher from the lift head by moving it straight up with tweezers WARNING Injury by sharp metal edges Pre punchers are sharp and might result in injury To prevent personal injury be careful when getting in contact with sharp metal areas 9 Slide the open end of the teflon tubing over the outlet pre puncher using forceps or by grabbing it with sandpaper wrapped around it 10 Insert the outlet pre puncher in the lift head in a vertical movement 11 Tighten the plastic screw 12 Slide the liquid handling module back into the instrument until it is positioned by internal pins 13 Tighten the two screws fto fix the liquid handling module 14 Mount the front cover and tighten the two screws to fix Agilent 7100 Capillary Electrophoresis System User Manual 139 5 i the Agilent 7100 Gapillary Electrophoresis System 1 z re CE p sis Plus High P apillary Electrophor Preparing the Agilent CE Instrument for High Pressure Use 1 Ensure that the gas source is switched off prior to connection 2 Connect the push fit connector and gas line to the receptor on the rear of the Agilent CE instrument Connect the gas line to the source 4 Switch
178. rior to using high pressure 9 Close and restart ChemStation to fully apply the changes 10 The activated CE p mode is displayed in the top line of the CE diagram and a new icon for an external pressure reservoir will appear see Figure 46 Instrument Control Easy Sequence Sequence Queue Easy Sequence Setup CHECKOUT_PHAP M WL BW RefWL RefBW mAU A 1920 4 3600 100 44 Outlet UO Inlet A 2 a B 2540 10 360 0 100 159 Cy C 235 0 10 3600 100 17 25 2 C Electrolyte Waste Actual Values Method Limits 0 0KV 0 0 KV OA 300 pA 0 0W 6 0 W d Time 11 00 min OGE00020 0 00 0 00 DE Figure 46 CE diagram with CEC mode activated 152 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 CEC Specific GUI Explanation When the CEC mode is selected a new icon in the form of a gas bottle appears on the left right side of the CE diagram next to the tray see Figure 46 on page 152 There are also a number of new parameters which may be set either from the Edit Entire Method option or from the Individual Parameter Settings within the Instrument menu selected from the Method amp Run Control toolbar The new parameter choices appear in the following menu selections CEC Method Setup When CEC mode is selected a new section for pressure settings appears on the left side of the Method Setup dialog box see Figure 47 on page 154 The parame
179. rizing the inlet vial and or the outlet vial External pressure is reduced to the set value between 2 and 12 bar and applied in a controlled manner Raw External Pressure Allows pressurizing the inlet vial and or the outlet vial The external pressure up to 15 bar can be applied directly without reduction CE p Control Using the CE Diagram High pressure may be applied independently of the method for capillary conditioning or cleaning by clicking the external pressure icon in the CE diagram see Figure 39 on page 142 This will cause a parameter setting field to appear see Figure 41 on page 145 From this field you may chose to apply pressure to the inlet outlet or both vials and also whether to simultaneously apply a voltage of 30 to 30 kV for a given time Agilent 7100 Capillary Electrophoresis System User Manual 147 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography CEC Capillary electrochromatography CEC is a fusion of capillary electrophoresis and capillary chromatography In CEC solvent is mobilized through a packed capillary column by electroosmotic flow instead of the hydraulic flow used in liquid chromatography LC Higher efficiencies are achievable because of the uniform flow velocity profile and the ability to use smaller particle sizes in longer columns than is possible with LC Operation of CEC requires an equal application of high pressure to the inlet and outlet vials in ord
180. rmation Identifying Instrument Components 172 7100 Capillary Electrophoresis Instrument Electronics 173 Firmware 176 Diode Array Detector 179 Changing the Fuses for the Power Supplies 182 Installing Drainage Tubing 183 External Water Bath for Tray Cooling Optional 184 Analog to Digital Converter 186 This chapter provides a more detailed description of the instrument s hardware and electronics RE Agilent Technologies 171 7 Hardware Information Identifying Instrument Components Identifying Instrument Components Cassette cooler Cassette Detector Insulation plate Replenishment needle Outlet lift Inlet lift Connector for status board Replenishment lift Sample tray Air pump Sa zt Electrolyte bottle Waste bottle Figure 52 Overview of instrument components 172 Agilent 7100 Capillary Electrophoresis System User Manual Hardware Information 7 7100 Capillary Electrophoresis Instrument Electronics 7100 Capillary Electrophoresis Instrument Electronics Fuses LAN connector USB connector Remote control Serial connector B CAN ow Configuration Analog In Out e Line fuses Line power Figure 53 Connectors on the rear of the instrument Agilent 7100 Capillary Electrophoresis System User Manual 173 7 174 Hardware Information All electrical connectors are placed on the right side of the instrument see Figure 53 on page 173
181. ront screws of the liquid handling module 2 Put in bottles 1 Push liquid handling module in Figure 70 Liquid handling module 11 Reconnect the line power drainage external pressure supply the connectors for an external cooling bath if applicable and switch on the instrument 12 Reestablish the connection to the instrument from the Lab Advisor software Select Start monitoring NOTE No high voltage can be applied if the front cover is removed Touching the electrodes inside the instrument is safe However under the following circumstances usage of the alignment center slow lifter movements with a low force are possible with the following alignment procedure When you start any lifter movement your hands should be outside the instrument Agilent 7100 Capillary Electrophoresis System User Manual 213 214 Maintenance 13 Open the CE Control screen under Tools in the Lab Advisor software Select the Alignment Center The alignment procedure can be started with the front cover un installed 14 Follow the procedure to align the inlet and outlet electrode and the replenishment needle The procedure consists of a prepare step and an alignment check The alignment is done while the pre puncher of the respective lift is raised until it is near the electrode or needle tip prepare button Now you can insert the capillary of the electrode alignment tool from the funnel of the electrode With careful puncture you ca
182. rophoresis System User Manual 4 LAN Configuration What You Have to Do First 62 TCP IP Parameter Configuration 63 Configuration Switch 64 Initialization Mode Selection 65 Link Configuration Selection 69 Storing the Settings Permanently with Bootp 70 Manual Configuration 71 Agilent Bootp Service 75 Location of the Media Access Control MAC Address 76 This chapter provides information on connecting the CE instrument to the Agilent ChemStation PC RE Agilent Technologies 61 4 LAN Configuration What You Have to Do First 62 The instrument has an on board LAN communication interface Please review the following options 1 The instrument will be connected to the LAN Ask your administrator to provide you with an IP address a subnet mask address and a gateway address Proceed with manual configuration see Manual Configuration on page 71 You will have to initially establish a LAN connection using the default settings of the instrument and a crossover LAN cable see Table 19 on page 68 2 The instrument and PC will be set up locally via a crossover cable or a hub No addresses are needed Proceed with establishing a LAN connection using the default settings of the instrument see Table 19 on page 68 3 Identification of the instrument in your LAN by MAC address If you plan to let a Bootp service see Agilent Bootp Service on page 75 assign an IP address please continue with locating the Media Access
183. ry seats The capillaries should slide directly into the capillary seats Otherwise they might break or be damaged Make sure the ends of the capillary are not bent when lowering the capillary cassette fyou have problems inserting the capillary into the electrodes check if they are blocked with salt crystals see Cleaning the Electrodes Pre punchers and Insulation Plate on page 196 5 Slowly lower the cassette until it reaches the insulation plate While lowering the cassette insert the capillary ends into the capillary seats of the electrodes Agilent 7100 Capillary Electrophoresis System User Manual 133 5 Using the Agilent 7100 Capillary Electrophoresis System CAUTION 6 Move the cassette guide back again while holding the cassette down The alignment interface aligns the capillary automatically with the detector Only alignment interfaces dedicated to the 7100 CE instrument will fit Interfaces from different instruments will not fit and will lead to unsatisfactory results Damage to the cassette Closing the top cover with an improperly installed cassette may damage the cassette Do not close the top cover if the cassette is not properly installed 134 7 Close the top cover and secure it by latching it onto the body of the case For safety reasons no power is applied if the top cover is open Because the cassette was changed the Agilent ChemStation automatically performs the wavelength calibration i
184. s underlined words To access specific information in the help When you are in the Agilent ChemStation or Lab Advisor software select a context sensitive element and press Fl When you are in the online help search for a specific keyword using the search command select a jump a jump is an underlined word you can select to go to a topic related to the word or select a word or phrase with a dotted underline to see its definition The online help also contains online information on how to use help 14 Agilent 7100 Capillary Electrophoresis System User Manual Where to Find Information Table 1 Where to find information Introduction 1 Task Refer to Installation of the Agilent CE System installing the external water bath installing the high sensitivity cell Setting up the Agilent CE System for CE analysis CE p How to use the Agilent CE System in the CE p mode How to use the Agilent CE System for capillary electrochromatography How to maintain the Agilent CE System Setting up a sequence Data analysis Setting up a report Spectral library search Setting up customized reports Safety information Learning ChemStation concepts Learning the theory of the technique Application The Core Agilent CE Instrument Installing the Instrument on page 53 Chapter Using the Agilent 7100 Capillary Electrophoresis System on page 77 Capillary Electrophoresis Plus High Pre
185. s and Specifications Site Requirements 18 Physical Specifications 21 Performance Specifications 22 Anforderungen an den Aufstellungsort 23 Abmessungen und Gewicht 26 Leistungsspezifikationen 27 Exigences d installation 28 Caracteristiques physiques 31 Caracteristiques de performance 32 Requisiti ambientali 33 Specifiche fisiche 36 Specifiche delle prestazioni 37 Requisitos de las instalaciones 38 Especificaciones fisicas 41 Especificaciones de rendimiento 42 43 46 47 48 51 52 This chapter provides information on the environmental requirements as well as physical and performance Specifications RE Agilent Technologies 17 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the 7100 Capillary Electrophoresis instrument Before you begin installation check carefully that the place you have chosen meets the requirements below Power Consideration The instrument power supply has wide ranging capabilities and accepts any standard line voltage in the range mentioned in Table 2 on page 21 Consequently there is no voltage selector on the back of the detector The module is partially energized when switched off as long as the power cord is plugged in Repair or maintenance work on the module can lead to personal injuries for ex
186. serve proper safety precautions Agilent 7100 CE instrument covers have not been designed as bioseals for aerosols or liquid containment Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of microorganisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Always follow local state and federal biohazard handling regulations when disposing of biohazardous waste material Handle all infectious samples according to good laboratory pocedures and methods to prevent spread of disease Dispose of all waste solutions and products according to appropriate environmental health and safety guidelines 256 Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 pesi This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types
187. sige 5 40 C Umgebungstemperatur bei Lagerung Umgebungstemperatur 40 bis 70 C bei Lagerung Luftfeuchtigkeit unter 80 bei 31 C Nicht kondensierend Betriebsh he bis zu 2000 m Externe K hlung max 0 5 bar 7 2 psi max 50 C Wasserbad Externer Druck 2 12 bar 29 174 psi lfreie Luft oder Stickstoff Sicherheitsstandards IEC Installationskategorie Il Nur f r den Einsatz im CSA UL Verschmutzungsgrad 2 Innenbereich geeignet Geh use Alle Materialien sind recyclebar F r Applikationen im CEC Modus Capillary Electrochromatography oder CE p Modus Kapillarelektrophorese Modus mit optionaler Verwendung h heren Druckes kann eine externe Druckversorgung mit lfreier Luft oder Stickstoff und einem Druckmaximum von 15 bar 218 psi verwendet werden Ein entsprechender m nnlicher Adapter f r den weiblichen Adapter des Ger ts und PTFE Schl uche werden mitgeliefert Das Agilent 7100 Kapillarelektrophorese System ist auf den Betrieb in einer typischen elektromagnetischen Umgebung ausgelegt in deren unmittelbarer N he keine HF Sender wie z B Mobiltelefone verwendet werden d rfen Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Leistungsspezifikationen Tabelle 5 Leistungsspezifikationen f r das Agilent 7100 Kapillarelektrophorese System Typ Spezifikationen Kommentare Sicherheitsvorkehrungen Steuerung und Datenauswertung Kommunikation GLP Ei
188. sis System User Manual LAN Configuration 4 Agilent Bootp Service Agilent Bootp Service provides central administration of IP addresses for Agilent CE instruments in a LAN The service runs on the instrument LAN PC which must be running a TCP IP network protocol and cannot run a DHCP server When an instrument is powered on a LAN interface in the instrument sends a request for an IP address or host name and provides its hardware address as an identifier The request may continue for up to 5 minutes The Agilent Bootp Service answers this request and passes a previously defined IP address and host name associated with the hardware address to the requesting instrument When the instrument receives its IP address and host name it stops sending the request It maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent Bootp Service must be started Since the Agilent Bootp Service runs in the background the instrument will receive its IP address once it is powered up Detailed documentation on installation and the usage of the Agilent Bootp Service can be found on your ChemStation installation DVD under Support Manuals Installation Agilent 7100 Capillary Electrophoresis System User Manual 75 4 LAN Configuration Location of the Media Access Control MAC Address 76 The MAC or hardware address of the LAN interfaces is a world wide unique identifier No ot
189. sor to align and check the installation If the funnel shows grooves replace the pre punchers WARNING Risk of injury due to sharp tips The pre punchers have very sharp tips Be careful when touching the tips of the pre punchers 1 Flush the pre punchers with water Watch for salt crystals All salt crystals must be removed Take particular care with the teflon tube connector Figure 65 Flushing pre punchers 2 Flush the pre punchers with water and isopropanol 3 Use compressed air to dry the pre punchers and remove all traces of liquid Do not forget to dry the connected tubes as well 206 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 If there is an accumulation of dirt in the pre puncher funnel put the pre punchers and connecting tubing in a beaker of water and treat it in an ultrasonic bath for five minutes followed by steps 2 and 3 in this section Residual liquid in connecting tubes may be transported to the air valve blocks and result in damage Such liquid plugs may also negatively influence injection reproducibility Cleaning the Insulation Plate Salt crystals films of substances such as buffer or gel that allow moisture will lead to increased leakage current or even arcing The insulation plate must be free of such contamination 1 Moisten a tissue with water and use it to clean the insulation plate 2 Moisten a tissue with isopropanol and use it to clean the insula
190. ssure CE p on page 136 Capillary Electrochromatography CEC on page 148 Chapter Maintenance on page 189 Online help Understanding Your ChemStation Online help Understanding Your ChemStation Online help Understanding Your ChemStation ChemStation Applications handbook and online help ChemStation Applications handbook Safety information in Appendix on page 231 Understanding Your ChemStation handbook High Performance Capillary Electrophoresis An Introduction The CE Partner CD ROM The comprehensive interactive tool for beginners and advanced users of CE Agilent web page http www agilent com search for Application notes Agilent 7100 Capillary Electrophoresis System User Manual 15 1 Introduction What Learning Products are Available 16 The Agilent CE System is supplied with the following e User s Guide High Performance Capillary Electrophoresis An Introduction The Agilent ChemStation and Lab Advisor Instrument Utilities software DVD is supplied with the following electronic documents Installing Your Agilent ChemStation for LC and CE Systems Installing and Understanding Your Spectra Module e Understanding Your Agilent ChemStation Online help Understanding Your Agilent ChemStation for CE Systems Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System User Manual 2 Site Requirement
191. strument Agilent 7100 Capillary Electrophoresis System User Manual 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance Appendix This chapter provides additional information on safety legal and web content associated with your 7100 CE instrument Agilent 7100 Capillary Electrophoresis System User Manual Contents Introduction 13 Introduction to the Agilent 7100 Capillary Electrophoresis System 14 Getting Help 14 Where to Find Information 15 What Learning Products are Available 16 Site Requirements and Specifications 17 Site Requirements 18 Power Consideration 18 Power Cords 19 Bench Space 20 Environment 20 Physical Specifications 21 Performance Specifications 22 Anforderungen an den Aufstellungsort 23 Stromversorgung 23 Netzkabel 24 Platzbedarf 25 Umgebungsbedingungen 25 Abmessungen und Gewicht 26 Leistungsspezifikationen 27 Exigences d installation 28 Remarques propos de l alimentation 28 C bles d alimentation 29 Encombrement 30 Environnement 30 Caract ristiques physiques 31 Caract ristiques de performance 32 Agilent 7100 Capillary Electrophoresis System User Manual Contents Requisiti ambientali 33 Alimentazione 33 Cavi di alimentazione 34 Spazio necessario 35 Ambiente 35 Specifiche fisiche 36 Specifiche delle prestazioni 37 Requisitos de las instalaciones 38 Consideraciones sobre la corriente 38 Cables de alimentaci n 39 Espacio
192. t Using the Wash Inlet Electrode commands leads to injection of a small volume on the outlet side equaling approximately 50 mbar x 3 s for a 50 um capillary Therefore position at the outlet side a vial with run buffer or any other suitable run buffer vial Empty vials at the outlet lead to air in the capillaries which needs to be flushed out again Usage of short electrodes part number G7100 60033 in combination with pressure injections also reduces this type of take over greatly insert reference to injection section Short electrodes are shorter by 5 mm sample is filled only at low level and so the electrode does not touch the sample Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Injection You have several choices for CE injection Hydrodynamic injection by pressure or vacuum Electrokinetic injection applying a voltage current or power Using an injection program The injection parameters can be programmed using an expandable table in the right side of the CE Method Setup screen The associated timetable can be used to apply pressure apply voltage or flush between vials Inlet and outlet vials can be loaded and wait time can be programmed here After the injection has finished the analysis starts with recording detector signals to result file Hydrodynamic Injection With hydrodynamic injection the inlet buffer reservoir is replaced with the s
193. tector Test suite in the Lab Advisor Instrument Utilities software to determine the initial lamp intensity of your new lamp Store the result for you own reference Further Action If the instrument fails this test check the following correct seating and connection of the lamp cables correct seating of the cassette and if the green alignment interface without capillary is used check for contamination of the interface If none of these reasons apply call Agilent Technologies Agilent 7100 Capillary Electrophoresis System User Manual 227 Maintenance Cleaning the Instrument The instrument case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the instrument Risk of instrument damage Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module Do not use an excessively damp cloth during cleaning Drain all solvent lines before opening any fittings 228 Agilent 7100 Capillary Electrophoresis System User Manual Agilent 7100 Capillary Electrophoresis System M User Manual eve Parts and Materials for Maintenance amp e Overview of Maintenance Parts 230 This chapter provides information on the parts required for maintenance RE Agilent Technologies 229 9 Parts and Mater
194. ter set here is the pressure applied to both vials in the inlet and outlet lift during the electrophoretic run Options that can be selected include Pressure off Internal pressure or vacuum up to 100 mbar can be applied to the inlet lift Raw External Pressure External set pressure from 2 12 bar Agilent 7100 Capillary Electrophoresis System User Manual 153 5 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography CEC Setup Method y DAD CE instrument Curves Vials Replenishment Inlet Home 04 buffer Preconditioning Outlet Home 05 buffer Injection Add method vial Parameter injection Ho Cassette Temperature Not controlled O 200 High Voltage System V Enable High Voltage Voltage 00 KV positive Polarity Current 30 wn Power 60 w Low Current alarm limit ve 100 MA Pressure Of Internal 0 mi Raw External Pressure External 20 a Stoptime Posttime As Injector No Limit 9 of e 1000 min 100 min E een Insert Delete Ciearall wes _cw co f Move sown Timetable Postconditioning Show timetable graph Figure 47 CEC setup method screen Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Preconditioning and Postconditionin
195. th the HV icon a battery in the CE dashboard the active instrument method must have Enable High Voltage check marked The maximum stored energy in the CE power supply is below 200 mJ at 30 kV Due to this and the fixed current limit of 300 uA the high voltage applied is not defined as hazardous voltage Positive voltage settings will configure the electrode at the inlet vial with positive values positive polarity Positive polarity is regarded as the standard setting You can reverse the polarity to negative by entering negative values for high voltage This means that a negative voltage is applied at the inlet electrode The outlet electrode is always grounded to ensure that the potential at the point of detection is close to ground Even with negative polarity the inlet electrode is the point where the voltage is applied and the outlet electrode is close to ground Although the outlet electrode is always at the ground potential you should make sure that the electrode is not short circuited to ground The electrode is connected to the power supply to measure the current flowing through the capillary If the ground electrode is short circuited to ground all current flowing through the capillary is signaled as leakage current It is good CE practice to ramp up voltage instead of switching voltage suddenly to high values This is regarded as gentle treatment for samples which might otherwise be affected by the field strength such as pr
196. tion and the Lab Advisor Instrument Utilities software The CE instrument doesn t have a keyboard itself It is completely controlled by the ChemStation through the CE and DAD diagram For detailed description of data evaluation see the manuals Understanding Your ChemStation and Agilent ChemStation for CE System Installing and Understanding 78 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Controlling the Agilent CE Instrument Before You Start the Agilent CE Instrument Use the following as a checklist before you start the Agilent CE instrument Make sure that the replenishment bottles are installed and properly tightened the cassette with a capillary installed is inserted in the system the cassette door and the tray door are closed and you do not need to put the vials into the tray before you start the Agilent CE instrument Starting Up the Agilent CE Instrument The power switch of the instrument is located on the left front corner of the instrument Press this switch to turn on the line power After the instrument is started the fans and the pump start working The instrument starts to build up internal pressure approximately 950 mbar first followed by a vacuum The pump becomes idle and switches on only occasionally once the pressure and vacuum setpoints are reached During initialization the instrument tests different functions
197. tion plate 3 Dry the insulation plate with a tissue and compressed air Agilent 7100 Capillary Electrophoresis System User Manual 207 8 Maintenance Reinstalling the Pre punchers 1 Carefully insert the pre puncher labeled 8 in the outlet lift Connect the tube to its connector labeled 8 if you run applications in the CE p and CEC mode The fitting of the outlet pre puncher must be tight Otherwise problems may occur during CEC mode If you do not run applications in CE p and CEC mode keep the tube disconnected and fix the tubing in such way that it does not disturb the movement of the inlet lifter Figure 66 Inserting the pre puncher 2 Carefully insert and tighten the plastic screw to secure the pre puncher The pre puncher will assume the correct position as the screw is tightened 3 Carefully insert the inlet pre puncher with the air pressure tube labeled 7 connected in the inlet lift Connect the tube to its connector labeled 7 The fitting of the inlet pre puncher must be tight Otherwise pressure or injection problems may result 4 Carefully insert and tighten the plastic screw to secure the pre puncher The pre puncher will assume the correct position as the screw is tightened 208 Agilent 7100 Capillary Electrophoresis System User Manual Maintenance 8 The screws used for fixation of the inlet and outlet pre puncher are made of plastic Do not use metal screws Metal screws may lead to leakage current an
198. to the 7100 CE instrument is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session Please refer to Using Default on page 67 for establishing an initial LAN connection 1 Open the system DOS prompt window by clicking the Windows START button and select Run Type cmd and press OK 2 Type the following after the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt E C WIND OWS system32 cmd_exe Cin gt telnet 134 40_279 95 Figure 8 Telnet starting a session where lt IP address gt may contain the assigned address from a Bootp cycle or the default IP address see Configuration Switch on page 64 If the connection is established successfully the instrument responds with the following E C AWINDOWS system32 cmd exe telnet 134 40 27 95 Agilent Technologies G1315C PPB9060824 Figure 9 A connection to the instrument has been made Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration 4 3 Type and press Enter to see the available commands CAWINDOWS system32 cmd_exe telnet 134 40 27 95 Agilent Technolo s G1315C PP9B8ANAG24 command syntax description display help info dicplay current LAN cettinge IP Addr Subnet M set Default exit shell Figure 10 Telnet commands Table 21 Telnet commands Value Description Displays syntax and descriptions o
199. ttle and the waste bottle is approximately 5 mL Please note that a relatively high amount of buffer is located in the glass frit of the electrolyte bottle It is recommended that you use the Flush Tubes command first to flush the direct tubing connection from the electrolyte bottle to the waste bottle with at least for example 10 times the volume of fresh buffer Please note the individual viscosity of your buffer system If the replenishment system will not be used for some time it should be cleaned first with water and then with isopropanol by the Flush Tubes and Clean Tubes operations Clean Level Sensor The purpose of the Clean Level Sensor functionality is to dissolve small buffer crystals which may have formed in the tip of the level sensing needle short needle of the double needle in the replenishment needle assembly Remaining pathways of the internal tubing of the level sensing circuit are also cleaned One indication to use this function is failed level sensing during operation The other indication is a failed IRP Injection Replenishment and Pressure level sensing test from the injection replenishment system tests of the Lab Advisor You may use this procedure as well if you leave the replenishment systen unused for a while This function prompts you to load tray position 48 or user input with a vial filled with an appropriate cleaning liquid usually water or current buffer system When initiated 8 cleaning cycles are perform
200. tuez chaque tape de la pro c dure d installation Par ailleurs vous devez respecter les consignes suivan tes Ne retirez pas les capots de l instrument pendant son fonctionnement Avant la mise sous tension de l instrument toutes les bornes de mise la terre ral longes lectriques transformateurs et appareils qui y sont raccord s doivent tre reli s une terre de protection par le biais d une prise de masse Toute interruption de la connexion la terre de protection cr e un risque d lectro cution pouvant entrainer des blessures graves Si l int grit de cette protec tion devient suspecte l instrument doit tre mis hors service et son utilisation doit tre interdite Assurez vous que les fusibles sont remplac s uniquement par des fusibles courant nominal sp cifi et de type sp cifi fusion normale temporis s etc Ne pas utiliser de fusibles r par s et ne pas court circuiter les porte fusibles Agilent 7100 Capillary Electrophoresis System User Manual Appendix 10 Certains des r glages d crits dans le manuel sont effectu s sur un instrument sous tension dont les capots de protection ont t retir s Les potentiels pr sents en de nombreux points peuvent causer des blessures Il convient d viter dans la mesure du possible d effectuer des op rations de r glage d entretien et de r paration sur un instrument ouvert sous tension Si c est in vitable ces op rations doivent tre effectu
201. ture fluctuations could cause condensation within the module fyour module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 20 Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications 2 Physical Specifications Table2 Physical specifications Type Specification Comments Weight 35 kg 77 2 Ibs Dimensions 350 x 510 x 590 mm width x depth x height 13 8 x 20 1 x 23 2 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 350 VA 300 W 1024 BTU h Maximum Ambient operating 5 40 C 41 104 F temperature Ambient non operating 40 to 70 C 40 to 158 F temperature Humidity below 80 at 31 C 87 8 F Non condensing Operating altitude Up to 2000 m 6500 ft External cooling max 0 5 bar 7 2 psi Waterbath max 50 C 122 F External pressure 2 12 bar 29 174 psi Oil free air or nitrogen Safety standards IEC Installation category Il For indoor use only CSA UL Pollution degree 2 Housing All materials are recyclable For applications run in CEC mode Capillary Electrochromatography or CE p mode Capillary Electrophoresis mode with optional usage of higher pressure an external pressure supply of oil free air or nitrogen with a maximum pressure of 15 bar 218 psi can be used A respective male adapter that fits into the instrument s f
202. ual contains technical reference information about the Agilent 7100 Capillary Electrophoresis System The manual describes the following introduction and specifications installation using and optimizing troubleshooting maintenance parts identification safety and related information Agilent Technologies 2009 Printed in Germany 12 2009 G7100 90000 Agilent Technologies
203. upply Select under Instrument in the CE diagram the Instrument Configuration Menu see Figure 44 Instrument Configuration Configurable Modules Selected Modules A Agilent CE G7100A Auto cum Configuration em il CE Part til CE Part G7150A PP00000007 Y DAD Part N 4 DAD Part G7151A PP00000007 amp BnPump lsoPump eb QuatPump Generic Ask for configuration change at ChemStation startup Figure 44 Configuration menu 150 Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System 5 Capillary Electrochromatography CEC 6 Enable the CE p mode by selecting the option External Pressure Installed CE Part Configuration Instrument 1 G7100 Analog In 1 000000 Figure 45 Select CE mode to decide which access to config is used 7 Confirm your selection by clicking OK 8 Make sure tube 8 is connected see Outlet Lift Connection to the Tubing System on page 138 Agilent 7100 Capillary Electrophoresis System User Manual 151 5 Using the Agilent 7100 Capillary Electrophoresis System anillarv Elartrachrn tanrank Capillary Electrochromatography CEC WARNING Damage or contamination of pressure valves Make sure tube 8 is connected properly before you apply high pressure to any lift High pressure may flush liquid into the pressure valves and lead to permanent damage Connect tube 8 p
204. uso futuro Entrada y salida anal gicas Caracter sticas de GLP Mantenimiento preventivo asistido EMF registros electr nicos de mantenimiento y errores 42 Agilent 7100 Capillary Electrophoresis System User Manual 7100 y 46 Site Requirements and Specifications 2 ni DR 12
205. xample SDS the waste bottle should contain some liquid for example a few mL of isopropanol to destroy the detergent bubbles Detailed information on buffers is given in the primer High Performance Capillary Electrophoresis 5990 3777EN delivered together with the instrument or numerous application notes available on our web page Make sure the waste bottle is empty so it can hold the same amount of liquid as in the electrolyte bottle Agilent 7100 Capillary Electrophoresis System User Manual 111 5 Using the Agilent 7100 Capillary Electrophoresis System CAUTION Instrument damage by corrosion Components of the replenishment system can be damaged by aggressive or corrosive buffers organic solvents or evaporated components from these Avoid using aggressive or corrosive buffers or organic solvents that can damage the liquid handling system Buffers for replenishment or evaporated components from these buffers will come into contact with the following material polytetrafluoroethylene PTFE stainless steel SST Grade 316 fluorine rubber FPM Viton polyaryletheretherketone PEEK perfluoro ethylene propylene FEP glass filter bottles A typically acceptable pH range is pH 3 11 for example formic acid can be used in concentrations up to 0 1 e If you would like to use a buffer composition exceeding the given conditions we recommend running an analysis without using the replenishment system but individual vials

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