Leica DM300 User Manual
Contents
1. 6 Hold the knurled ring on the objective nosepiece and swing the objective back and forth to eliminate air bubbles Then bring the oil objective into the final posi tion so that the oil drop is between the front lens of the objective and the speci men slide Leica DM 300 User Manual 31 Oil Immersion Technique cont 7 Look through the microscope and slowly rotate the fine adjustment knob so that the specimen stage moves upwards until the specimen is in focus 8 If you are finished working with the oil immersion objective clean the front side of the objective the specimen slide and all other surfaces that have come into contact with the oil following the instructions in the Care of the Microscope section on page 35 Leica DM300 User Manual 32 Care of the Microscope Maintenance instructions General Notes Cleaning coated Parts and plastic Parts e Protect the microscope from moisture e Dust and dirt particles should be removed fumes and acids and from alkaline caustic and corrosive materials Do not store with a soft brush or lint free cotton cloth e Remove coarse debris with a moistened chemicals in the vicinity disposable cloth Protect the microscope from oil and grease Acetone xylene or nitro containing thin Never grease or oil mechanical parts or ners must NOT be used sliding surfaces 3 e Never use chemicals to clean colored Follow the instructions of the disinfectan2. Leica DM300 User Manual Heica MICROSYSTEMS Congratulations Congratulations on purchasing the DLeica DM300 Compound Microscope This model s exclusive design features and full range of accessories make it a truly versatile high quality instrument for educational and routine clinical applications Though the reliability and robustness of Leica microscopes is already practically proverbial they still require a certain degree of care and attention Therefore we recommend that you read this User Manual It contains all the infor mation you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your microscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work Leica DM300 User Manual Contents Congratulations 2 Contents 3 Switching on the Microscope 24 Selecting an Objective 25 Preparing for Viewing 26 Symbols Used in this User Manual gt Focusing 27 Important Safety Notes 6 Adjusting the Binocular Tubes 28 Important Safety Notes continued 7 Adjusting the binocular tubes continued 29 Instructions for Use 8 Adjusting the Eyecups 30 Instructions for Use continued 9 Oil Immersion Technique 31 Health risks and dangers of use 10 Oil Immersion Technique cont 32 Informa
3. contact us as follows Contact address Leica Microsystems Schweiz AG Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Leica DM300 User Manual 12 Electrical Data and Ambient Conditions Fuse Replacement Unplug the instrument before changing any fuses The Leica DM300 has two fuses which are located behind the power cord receptacle Only use fuses of the following type 5x20 mm 1 0 A 250V high breaking capacity 13RFAG30003 Electrical Data Input 100 240 V 50 60 Hz 5 W 3 W LED General safety notes This instrument of safety class 1 has been built and tested in accordance with the following safety requirements for electrical equipment for measu rement control and laboratory use EN 61010 1 2002 08 EN 55011 2007 A2 2010 05 EN 61010 2 2008 06 EN 60825 1 2008 05 IEC 61010 1 2010 06 IEC 60825 1 2007 03 EN 61326 1 2006 10 LED Class 1 EN 61326 2 6 2006 10 A In order to maintain this condition and to ensure safe operation the user must follow the instructions and warnings contained in this instruction manual Environment Temperature for use 10 C 40 C Storage temperature 20 C 52 C 50 F to 104 F Manipulation shock 25 mm on 50 mm hard wood Transport shock unpacked 100 g 6 ms Transport shock packed 800 mn free fall Transport vibrations unpacked 5 200 Hz 1 5g Air pressure during use and storage 500 1060 mBar Humidity during u
4. servicing the product User Manual After service work or technical modifica tions the device must be readjusted in accordance with our technical specifica tions If the unit is modified or serviced by unau thorized persons is improperly maintained as long as maintenance was not carried out by Leica Microsystems or is handled improperly Leica Microsystems will not accept any liability The buildings electrical wiring must comply with the relevant national stan dard in other words we recommend using a current operated ELCB system RCB Accessories Maintenance and Repair Accessories Only the following accessories may be used with the Leica DM300 e The Leica Microsystems accessories descri bed in this User Manual Maintenance e The Leica DM300 microscope is basically maintenance free To ensure that it always operates safely and reliably we recommend that you take the precaution of contacting the responsible service organization Repairs and Service Work Only original Leica Microsystems spare parts may be used Before opening the instruments switch off the power and unplug the power cable Avoid contact with powered electrical e Other accessories provided that these have We recommend concluding a service circuits which can lead to injury been expressly approved for use by Leica contract with Leica Microsystems o with regard to safety tech Service Address REC In case of problems please
5. Caution Hazardous radiation may occur if controls adjustment jigs or procedures other than those outlined in this User Manual are used Information for the Person Responsible for the Instrument Information for the Person Responsible for the Instrument Ensure that the Leica DM300 is used only by persons qualified to do so Ensure that this User Manual is always available at the place where the micro scope is in use Carry out regular inspections to make certain that the authorized users are adhe ring to safety requirements When instructing new users do so thoroughly and explain the meanings of the warning signs and messages Allocate responsibilities for commissio ning operation and maintenance Monitor compliance with this Do not use the Leica DM300 unless it is in perfect condition Leica DM300 Inform your Leica representative or Leica Microsystems Schweiz AG Industry Divi sion 9435 Heerbrugg Switzerland imme diately of any product defect that could potentially cause injury or harm If you use accessories from other manu facturers with the microscope make sure that these manufacturers confirm that the combination is safe to use Follow the inst ructions in the User Manual for those acces sories Modifications to or service on the micro scope may be carried out only by technici ans who are explicitly authorized by Leica to do so Only original Leica replacement parts may be used in
6. ceito de seguranca Bezpe nostn pokyny Varnostni koncept Turvallisuus S kerhetskoncept k A LEM e He BS SRA Important Safety Notes continued Legal Regulations Adhere to general and local regulations rela ting to accident prevention and environmental protection EC Declaration of Conformity Electrically operated accessories are construc ted based on the state of the art of technology and are provided with an EC Declaration of Conformity Leica DM300 Disconnecting from the Power Supply Always position the Leica DM300 so that you can disconnect it from the power supply at any time The power cable must remain accessible at all times because the power cable is intended as a power disconnect device Plug Never install any other plug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions User Manual Instructions for Use This User Manual describes the Leica DM300 and includes important instructions related to operating safety maintenance and accessories Your Leica DM300 includes an interactive CD ROM with all relevant User Manuals in additional languages Keep it in a safe place and readily accessible to the user You can also download User Manuals and updates from our website at www leica microsystems com Leica DM300 Intended Use The Leica DM300 is an optical instrument for improving the visibility of objects th
7. ectives with magnification levels 4x 10x and 40x possibly also with a 100x objec tive Otherwise this can be retrofitted at any time Leica DM300 A Always turn the objective nosepiece by its knurled ring Never turn the objective nosepiece by using an objective as a handle Removing Objectives 1 Unscrew the objectives to remove them User Manual The proper Sequence When inserting the objectives you can begin with any available space on the microscope Continuation of the sequence however has to be selected such that the magnification always increases when turning clockwise Continued on next page Removing and Inserting Objectives continued Inserting Objectives 1 Screw the 4x objective into any available space on the objective nosepiece 2 Turn the objective nosepiece clockwise one unit 3 Screwin the next largest objective 4 Repeat steps 2 and 3 until all objectives have been installed Leica DM300 User Manual Installing the Mirror optional H The mirror is available as an optional Installing the Mirror Removing the Mirror accessory By means of this the ambient 1 Position the microscope at a well lit loca 1 Simply pull the mirror out of the holder light can be used for illuminating the sample if tion for example before a window no power source is available for the built in LED 2 Press the mirror onto the mount of the illu illuminator minator A The mirror i
8. es are already integrated in H the Leica EZTube and preset for this reason the eyepieces do not require adjust ment or assembly Leica DM300 Assembly 3 Turn the tube in the direction you desire 1 Loosen the setscrews at the left and right on the stand using the Allen key provided 4 Tighten the set screws on the left and right without applying too much force 2 Insert the dovetail of the binocular tube into the mount User Manual 17 Installing the Monocular Tube Tools used Assembly 3 Turn the tube in the direction you desire e Allen key 1 Loosen the setscrews on top of the stand using the Allen key provided This User Manual shows the binocular H tube from this point on However hand ling the microscope is the same for both Possi ble deviations are mentioned on the respective 4 Tighten the set screws on the left and right without applying too much force paT 2 Insert the dovetail of the monocular tube into the mount Eyepieces The eyepieces are already integrated in H the Leica EZTube and preset for this reason the eyepieces do not require adjust ment or assembly Leica DM300 User Manual 18 Removing and Inserting Objectives The objectives are already installed H on the Leica DM300 at delivery The following description shows how to un install the objectives if they have to be removed for cleaning or replaced by other objectives Your Leica DM300 is delivered with H obj
9. g to the User Manual can be assembled and disassembled by the customer should be packed separately Disposal Disposal must comply with locally applicable laws and regulations User Manual Health Risks and Dangers of Use Health Risks Workplaces with microscopes facili tate and improve the viewing task but they also impose high demands on the eyes and hand muscles of the user Depending on the duration of uninterrupted work at such a workplace impaired vision and problems with the musculoskeletal system may occur For this reason appropriate measures for reduction of the workload must be taken e Optimum workplace layout e Frequent changes of activity e Thorough training of the personnel giving consideration to ergonomic and organiza tional aspects Leica DM300 The ergonomic optical design and construction of the Leica microscopes are intended to reduce the exertion of the user to a minimum Danger of Infection Direct contact with eyepieces can be a A potential transmission path for bacterial and viral infections of the eye A The risk can be minimized by the use of personal eyecups User Manual Dangers during Use e The Leica DM300 may be connected to a grounded socket only e The Leica DM300 may be used when it is in proper working condition only LED Class 1 LED 1 Laser Product The illumination meets laser class 1 require ments in accordance with IEC 60825 1 Safety of Laser devices
10. he Condenser Selecting an Objective 2 Rotate the condenser diaphragm below the The condenser is furnished with an iris 1 Rotate the desired objective over the specimen stage so that the displayed value diaphragm which has to be adjusted to sample matches the selected objective match the effective numerical aperture of the individual objectives Leica DM300 User Manual 25 Preparing for Viewing Preparation 2 Insertthe specimen slide and slowly release 3 Using the X Y control position the speci 1 Open the specimen holder using the the tension lever men slide such that a part of the specimen tension lever is under the objective used Leica DM300 User Manual 26 Focusing Focusing 2 Move the specimen stage upwards as far as 3 Lookinto the eyepieces and adjust the illu 1 Usethe knurled ring to rotate the objective it will go using the coarse adjustment knob mination to a comfortable intensity nosepiece in such a way that the objec tive with the lowest magnification level is turned into the working position 4 Bring the specimen into focus using the fine adjustment knob Leica DM300 User Manual 27 Adjusting the Binocular Tubes 1 Adjust the tubes to your interpupillary The viewing tubes maintain a constant Open Binocular Tube distance Fold or unfold the eyetubes to tube length for all interpupillary settings If you are using an open standard tube you decrease or increase the distance between This mea
11. king through the eyepieces with both eyes The depth of field is lower for higher magnification levels Thus you will notice that if you switch to low magnification after bringing the microscope into focus with great magnification a minor adjustment of the fine focus may be required Continue with the Oil immersion section on page 31 User Manual Adjusting the Eyecups If you wear eyeglasses for microscope H viewing keep the rubber eyecups folded down If you do not wear eyeglasses you may find it useful to unfold the rubber eyecups in order to help block out ambient room light Leica DM300 User Manual Potential Sources of Infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye The risk can be minimized by the use of individual eyecups Eyecups can be ordered separately Please contact your Leica partner Oil Immersion Technique Oil Immersion Technique 3 Add a drop of immersion oil from Leica 5 Slowly move the specimen stage upwards 1 Search for the area on the specimen slide to the area of the specimen slide you are using the coarse adjustment knob until that you want to study studying the oil drop on the slide comes into direct contact with the lens of the oil immersion 2 Move the specimen stage down to the ii objective lowest position using the coarse adjust ment knob 4 Rotate the 100x objective to the working position
12. lease follow all safety instructions The instruments and accessories descri bed in this User Manual have been tested for safety and potential hazards Original Condition To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in this User Manual Leica DM300 Non intended Use Using the instrument in any way other A than that described in this User Manual may result in personal injury or damage to property Doing so can impair the functions of the protective measures provided The responsible Leica affiliate must be A consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this User Manual Unauthorized alte rations to the instrument or noncompliant use shall void all rights to any warranty claims User Manual Safety Concept Brochure The Safety Concept brochure contains additi onal safety information regarding service work requirements and the handling of the micro scope the electrical and other accessories as well as general safety instructions NIE Bezpe nostn koncepce Sikkerhedskoncept Sicherheitskonzept Ohutuseeskiri Safety concept Concepto de seguridad Concept de s curit Concetto di sicurezza Dro ibas noteikumi Saugos samprata Biztons gi el r sok Veiligheidsconcept Sikkerhetskonsept Koncepcja bezpieczenstwa Con
13. ng is required for a finger and moistened with window clea the entire life of the microscope ner e Cleaning 40x and 100x objectives requi res more care To attain the typical flatness for objectives with higher magnification the objective has a small concave front lens with a very small radius and slight curvature The surface of this front lens can be readily cleaned with a toothpick covered with a cotton tip or with a small cotton swab Wipe the front lens lightly without applying undue force or scrubbing action Make sure that the cotton tip contacts the concave lens surface Check the objective with a magnifier after cleaning Leica DM300 User Manual 36 Dimensions Leica DM300 User Manual 37 Dimensions all Dimensions in mm DM100 and DM300 identical e side view with binocular tube e front view with binocular tube e side view with binocular tube 180 rotated for storage 362 Leica DM300 User Manual 38 Dimensions all Dimensions in mm DM100 and DM300 identical e side view with monocular tube e front view with monocular tube e side view with monocular tube 180 rotated for storage m HI liiij amp Um 7 ul Leica DM300 User Manual 39 Leica DM300 User Manual 40
14. ns that a change of interpupillary need to make some adjustments the eyepieces until you see one illuminated distance does not affect parfocality magnifica circle tion or calibrations that depend on magnifica 1 Setthe focusing eyetube left side to 0 tion Leica EZ tubes If you are using a Leica EZ viewing tube which already has the eyepieces integrated with the eyetubes no additional adjustments are neces sary Be sure you are wearing your eyeglasses or contact lenses Continued on next page Leica DM300 User Manual 28 Adjusting the Binocular Tubes continued If you are comfortable wearing your H corrective lenses contact lenses or eyeglasses for microscope viewing leave them on and your adjustments will be minimal 2 Bring the specimen into focus using 10x magnification and the fine adjustment knob while looking through only the right eyepiece To help focus cover or close your left eye 3 Now look with your left eye through the left eyetube focusing eyepiece Now focus using the focus function of the eyetube When doing so do not change the height of the specimen stage 4 Rotate the knurled ring on the left eyetube until the specimen is sharply focused for the left eye This corrects for any vision differences between your right eye and left eye Leica DM300 5 Now switch to an objective with a higher magnification level no oil objective and bring the specimen into focus while loo
15. rough magnification and illumination It is used for observing Place of Use The Leica DM300 microscope may be used only in closed rooms and must be placed on a solid surface The Leica DM300 can be used at 10 C to 40 C Protect the microscope from oil chemi cals and extreme humidity If it is used outdoors the microscope must be protected from dust and moisture Never use electrical instruments outdoors and place them at least 10 cm away from the wall and from flammable substances User Manual Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Instructions for Use continued Accessories from third party Suppliers You can combine individual system articles with articles from external suppliers Please read the User Manual and the safety instructions from the supplier Integration in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for following all applicable safety instructions laws and guidelines Leica DM300 Transport If at all possible use the original packaging for shipping or transporting individual modules In order to prevent damage from vibrations all moving parts that accordin
16. s equipped with a flat and a concave surface Try them out to see which side works better 3 Look through the eyepieces and align the mirror so that it works best Leica DM300 User Manual Installing the Polarizer Kit optional The polarizer kit is not included in the delivery package but available as an 1 optional accessory Leica DM300 Assembly If you have already installed the tube Place the analyzer with the flat side facing down into the beam path The two screws have to rest in the two existing holes Install the tube User Manual Place the polarizer onto the aperture of the illuminator The handle must come to rest in the aperture with the white arrow poin ting upwards Turn the polarizer to align it The arrows indicate the current orientation Using the Leica DM300 Switching on the Microscope For safety reasons the power cable Startup Procedure 3 Switch on the microscope using the toggle is grounded via the microscope base 1 Attach the power cable to the rear side of switch on the rear side of the stand Never use an adapter between the power cable the microscope and the power supply that would render the grounding ineffective 2 Set the illumination to the lowest setting using the controller on the bottom left of the stand Work Surface A Always use the microscope on a hard stable surface Leica DM300 User Manual 24 Selecting an Objective Using t
17. se and storage 20 90 Installation Category Il Overvoltage Category Pollution degree 2 Leica DM300 User Manual 13 Assembling the Leica DM300 Unpacking e Carefully remove the microscope and any components from the packaging e Check the components against the planned configuration e Optional items such as contrast accessories camera adapters cameras and carrying cases are not shipped as part of the stan dard equipment These items are delivered in separate packages e Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise Leica DM300 User Manual 15 Identifying your Tube Identifying your Tube There are three types of viewing tubes Identify which viewing tube you have Assembly Installation of the tube is described on H page 17 using the binocular EZTube Other tubes however are installed exactly the same way Orientation H All tubes are connected via a dovetail and can be installed in any orientation Leica DM300 de Type 1 Monocular EZTube Type 3 Open binocular tube User Manual Type 2 Binocular EZTube Mounting the Binocular Tube Tools used e Allen key This User Manual shows the binocular H tube The installation of the monocu lar tube is shown on the next page However handling the microscope is the same for both Eyepieces The eyepiec
18. t manufacturer We recommend concluding a service contract with Leica Service Leica DM300 surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abra ded particles User Manual General Maintenance General Always use both hands to carry the microscope Leica DM300 Keep all optical components clean Clean liness is important for maintaining good optical performance When the microscope is not in use it should always be put away with protection against dust If any optical surface becomes coated with dust or dirt clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean User Manual e Optical surfaces should be cleaned using a lint free cloth with a commercially available glass cleaner Use solvent only very sparingly so that it does not flow out over the lens No part of the microscope is quite so vulnerable to collecting dirt dust and oil as the front lens of the objective Whenever you encounter lack of contrast cloudiness or poor defini tion carefully check the condition of the front lens with a magnifier General Maintenance cont e The lower magnification objectives have Illumination fairly large front lenses and can be cleaned e The Leica DM300 uses an LED illuminator with a cloth or lens tissue wrapped around Therefore no lamp changi
19. tion for the Person Responsible for the Instrument 11 Accessories Maintenance and Repair 12 Electrical Data and Ambient Conditions 13 Maintenance instructions 34 General maintenance 33 General maintenance cont 36 Unpacking 15 Identifying your Tube Dimensions Mounting the binocular tube 17 Dimensions all dimensios in mm DM100 and DM300 identical 38 Installing the Monocular Tube 18 Dimensions all dimensios in mm DM100 and DM300 identical 39 Removing and Inserting Objectives 19 Removing and inserting objectives continued 20 Installing the Mirror optional 21 Installing the Polarizer Kit optional 22 Leica DM300 User Manual 3 Important Notes Symbols Used in this User Manual Warning Safety hazard A This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following e Hazards to personnel e Instrument malfunctions and damage Leica DM300 Warning of hazardous electrical Voltage This symbol indicates information that must be read and observed Failure to comply can cause the following e Hazards to personnel e Instrument malfunctions and damage User Manual Important Information This symbol indicates additional infor mation or explanations that are intended to provide clarity Important Safety Notes A Before installing operating or using the instrument it is mandatory to read this User Manual In particular p
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