Home

User Manual

image

Contents

1. Refrigerated Models Nonrefrigerated Models Horizontal Fixed Angle Horizontal Fixed Angle Rotors Rotors Rotors Rotors 5 500 rpm 15 300 rpm 4 500 rpm 14 500 rpm Curve Accel Decel Accel Decel Accel Decel Accel Decel 9 0 54 0 40 0 47 1 04 0 25 0 23 0 44 0 47 8 0 54 0 29 1 20 1 17 0 27 0 24 1 06 1 09 7 0 56 1 03 2 29 2 28 0 29 0 26 2 03 2 12 6 1 40 1 39 4 55 4 52 0 30 0 30 4 11 4 12 5 2 30 2 26 7 39 7 17 1 04 1 07 6 13 6 14 4 3 18 3 11 10 02 9 42 1 08 1 11 8 07 8 05 3 4 54 4 48 15 16 14 33 2 13 2 18 11 12 11 26 2 9 41 9 35 29 07 30 56 4 31 4 34 23 17 23 20 1 14 40 14 11 45 56 46 16 12 03 12 17 40 00 40 16 0 14 40 14 11 61 01 46 16 12 03 12 17 40 00 40 16 Maximum speed for the S2096 microtiter rotor is 3000 rpm acceleration and deceleration times will decrease accordingly 3 9 Operation Setting Deceleration Rate STARTING A RUN 1 Press the ACCEL key The ACC DEC display will flash indicating that the selected rate number can be entered with the cursor keys 2 Press the A or Y cursor key until the required number is displayed The DECEL key is used to select deceleration rates that will maintain optimum separation When the DECEL key is pressed the ACC DEC display flashes indicating that one of the ten preset deceleration rates can be entered with the cursor keys The selected rate will depend on the type of run you are performing For pelleting runs where sample mixing is no
2. o oooooooooorororrr ro 1 3 E O A heath 1 4 HOUSING uses dd taa ds 1 4 Door er Bee ie See A 1 4 Rot r Ch mber 2 2 23 222 N aan 1 4 DIV GS if ape he OA ERTL 1 5 Temperature Sensing and Control 2 0000 1 5 Controls and Indicators 0 c eee eee eee ees 1 5 Power Switch 4280 sere a tots das 1 5 Control Panel 2 1 a a a a a ad E a a eee 1 6 SECTION 2 INSTALLATION Installing the Instrument 2 0 0 0 ec eee eee 2 1 Electrical Requirements 0 0 0 0 ccc ccc eens 2 2 Test Run ta ei ek Sad ee Ba Hr SE be bee in 2 4 111 Contents iv SECTION 3 SECTION 4 SECTION 5 APPENDIX Page OPERATION Run Pr ced te sizni A A nel 3 2 Preparation and Loading 00 00 cee eee eee eens 3 2 Entering Run Parameters 0 000 c eee eee eee 3 5 Starting R m gs 2 2 ea an ang 3 10 Changing Parameters During a Run o o ooooooo o 3 11 Stoppi s a RUN anregen 3 12 Unloading sata nasa 3 12 Summary of Run Procedures 0 0 0 cee ee eee eee 3 13 TROUBLESHOOTING User Messages o Musa A oO le ah Gai ed 4 1 Other Possible Problems 0 0 0 c cece eee eee eens 4 3 Emergency Access 1 2 0 c cece cette ence eens 4 3 CARE AND MAINTENANCE Maintenance bt tea ed kb ede Eat ea Gh ee 5 2 Preventive Maintenance 0 c eee eee eee ee 5 2 Replacing the Rotor Chamber Gasket 5 2 Cleaning un xe e eerie a
3. COULTER Allegra 21 Series GS 15 Series and Spinchron 15 Series Centrifuges Instruction Manual Symbol Symbol Symbole Simbolo Simbolo Title Titel Titre Titulo Titolo Qi ZF Dangerous voltage Gefahrliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo alta tensione EREE f K HEL As Attention consult accompanying documents Achtung Begleitpapiere beachten Attention consulter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate za ABA BROZ E ER WER MT AICHE On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione A SiR FF CEI Off power Aus Netzverbindung Arr t mise hors tension Apagado Spento fuori tensione Y EM Rx EM Protective earth ground Schutzleiteranschlu Liaison a la terre Puesta a tierra de protecci n Collegamento di protezione a terra RETA FEU trap Beth Earth ground Erde Terre Tierra Scarica a terra PA it Fe th SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation operation maintenance or repair of this instrument When you see the symbol on other pages pay spe
4. Install the new gasket by positioning the groove in the gasket over the rim of the opening Press the gasket around the opening so that the rim is seated in the gasket groove Figure 3 1 Replacing the Rotor Chamber Gasket CLEANING TUBE BREAKAGE Care and Maintenance Frequent cleaning is recommended to prolong the life of the centrifuge Always clean up spills when they occur to prevent corrosives or contami nants from drying on component surfaces To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or paper towel Clean the drive shaft shaft cavity threads and the tie down screw at least once a week using a mild detergent such as Beckman Solution 555 and a soft brush Dilute the detergent 10 to 1 with water Rinse thoroughly and dry completely Lubricate the drive shaft with Spinkote after cleaning Wash the bowl using a mild detergent such as Solution 555 Rinse thoroughly and dry completely Clean the centrifuge case and door by wiping with a cloth dampened with Solution 555 Do not use acetone or other solvents I gt NOTE Before using any cleaning or decontamination meth ods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Ifa glass tube breaks and all the glass is not contained in the b
5. obstructions 87 refrigerated models After the motor has cooled restart If the problem persists call Beckman Coulter Field Service 90 through 96 Temperature sensor Maximum deceleration Turn the power off O then back on I to malfunction to full stop reset refrigerated models 98 Rotor not Maximum deceleration Check that the installed rotor is usable recognized to full stop in the centrifuge If incorrect install an authorized rotor if rotor is authorized and problem persists call Beckman Coulter Field Service 99 Rotor recognized Maximum deceleration Input correct parameters for the rotor in use but incorrect to full stop then restart Troubleshooting to determine the nature of the problem and recommended actions If you are unable to correct the problem call your Beckman Coulter Field Service representative To help diagnose and correct the problem try to gather as much information about the situation as you can e Write down the error number that appears on the display e Note the operating situation when the error occurred rotor in use speed load type etc e Note any unusual environmental and or operating conditions ambient temperature voltage fluctuations etc e Add any other information you think may be helpful OTHER POSSIBLE PROBLEMS Possible malfunctions that may not be indicated by diagnostic messages are described in Table 4 2 along with pr
6. ALLEGRA 21 SERIES AND SPINCHRON 15 SERIES CENTRIFUGE WARRANTY Subject to the exceptions and upon the conditions specified below Beckman Coulter agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within one 1 year after delivery of the Avanti 64R Avanti 30 Allegra 21 GS 15 or Spinchron 15 series centrifuge the product to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Some components and accessories by their nature are not intended to and will not function for as long as one 1 year A complete list of such components or accessories is maintained at the factory and at each Beckman Coulter District Sales Office The lists applicable to the products sold hereunder shall be deemed to be part of this warranty If any such compo nent or accessory fails to give reasonable service for a reason able period of time Beckman Coulter will repair or at its election replace such component or accessory What consti tutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the
7. Description The Allegra 21 series GS 15 series and Spinchron 15 series centrifuges have been designed and tested to operate safety indoors at altitudes up to 2000 m 6562 ft Instrument safety features include the following The door has dual electromechanical door locking mechanisms to prevent operator contact with spinning rotors When the door is closed it locks automatically It can be unlocked only by pressing the OPEN DOOR key and opened only when the power is on and the rotor is at rest Two independent monitoring systems prevent the door from opening if the rotor is spinning A steel barrier surrounds the rotor chamber to provide full operator protection An overspeed system continuously monitors the rotor during centrifu gation The system includes a magnetic sensor on the drive motor and magnets imbedded in the rotors Throughout the run checks are made to ensure that the rotor does not exceed set speed An imbalance detector monitors the rotor during the run causing auto matic shutdown if rotor loads are severely out of balance At low speeds an incorrectly loaded rotor can cause imbalance Rotor insta bility can also occur if the centrifuge is moved while running or if it is not resting on a level surface Rubber pads on each of the centrifuge feet have been designed to minimize possible rotation in the event of a rotor mishap For additional protection an anchoring system is provided to secure the centrifuge in
8. also used to alert against unsafe practices A WARNING Used whenever an action or condition may poten tially cause serious personal injury or loss of life Mechanical damage may also result AN or A WARNING Indicates high voltage or risk of electric shock Turn the power switch off and disconnect the equipment from the main power source Refer servicing of all areas displaying either symbol to a service personnel Certain typographic conventions are used throughout this manual to distinguish names of user interface components such as keys and displays Key names for example START or ENTER appear in brackets e Display names for example TEMP C or SPEED appear in bold type e Cursor keys used to increment values up or down when setting parameters are shown as up and down arrows A or W Introduction CFC FREE CENTRIFUGATION To ensure minimal environmental impact no CFCs are used in the manufacture or operation of Allegra 21 GS 15 or Spinchron 15 series centrifuges RADIO INTERFERENCE CANADIAN REGULATIONS RECYCLING LABEL AZ A28219 AA NE Note On the instrument the triangle background is yellow rather than gray This instrument has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial enviro
9. can be set between 20 and 40 C refrigerated models only gt NOTE 1 Temperatures may vary slightly between instruments If sample temperature is crucial test temperature settings on your instrument using water samples Press the TEMP key The TEMP C display will flash indicating that the temperature can be entered with the cursor keys Press the A or Y cursor key until the desired run temperature is displayed Setting Acceleration Rate gt NOTE For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration For low temperature runs precool the centrifuge by running a 30 minute cycle at the desired temperature with a precooled rotor installed with the speed set at about 2000 rpm Operation The ACCEL key is used to select acceleration rates that will protect deli cate gradients When the ACCEL key is pressed the ACC DEC display flashes indicating that one of the 10 preset acceleration rates can be entered with the cursor keys The selected rate will depend on the type of run you are performing For pelleting runs where sample mixing is not a concern maximum acceleration can be used However if delicate gradients are being run a lower setting may be needed Acceleration rates are listed in Table 3 1 Table 3 1 Acceleration Deceleration Times in minutes seconds Times are approximate actual times will vary depending on rotor load
10. i ze GS 15R or CO Spinchron 15R Figure 2 1 Dimensions of the Nonrefrigerated and Refrigerated Centrifuges 2 3 Installation 2 4 TEST RUN To ensure safety the centrifuge should be wired to a remote emergency switch preferably outside the room where the centrifuge is housed or adjacent to the exit from that room in order to disconnect the instrument from the main power source in case of a malfunction A WARNING To reduce the risk of electrical shock this equip ment uses a three wire electrical cord and plug to connect the centrifuge to earth ground To preserve this safety feature Make sure that the matching wall outlet receptacle is properly wired and earth grounded Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge e Never use a three to two wire plug adapter e Never use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip gt NOTE The centrifuge must be plugged in and the power switch turned to on position I before the door can be opened We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment See Section 3 for instruc tions on operating the centrifuge After completing the test run return the pre addressed warranty card included with this literature This will validate the centrifuge warranty and ensure your receipt of fu
11. reason 1 Press the STOP key for normal deceleration as selected by the decel eration curve or Press the FAST STOP key for deceleration at the maximum rate see Table 3 1 I gt NOTE If you press FAST STOP the deceleration process cannot be interrupted the centrifuge can be restarted only after the rotor comes to a complete stop and the door is opened and closed 2 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the door latches then open the door gt NOTE To prevent chamber icing use a sponge to wipe condensation out of the chamber bowl between runs UNLOADING gt NOTE When you remove the rotor make sure that the tapered sleeve from the centrifuge drive shaft does not come out with the rotor If the tapered sleeve is inside the rotor drive hole remove it and put it back on the drive shaft see Figure 3 1 After completing a run unload the rotor following the instructions in the applicable rotor manual Operation If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Appropriate decontamination procedures should be applied to the centrifuge and accessories SUMMARY OF RUN PROCEDURES For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration For low temperature runs precool the centrifuge by running a 30 minute cycle with a precooled rotor i
12. revolutions per minute rpm or relative centrifugal field RCF can be used to select speed During centrifugation the SPEED display indicates the actual run speed of the rotor Setting RPM Setting RCF Operation gt NOTE 1 Use only the RPM mode to set run speeds for the H6002 bowl rotor In the bowl rotor RCF varies according to the tube in use as indicated in the rotor manual publication GS30 TB 002 If you use the RCF mode to set run speed the centrifuge will select an RPM level based on the 7 4 of the largest tube resulting in a speed too low to achieve the required RCF in the shorter tubes Also note that the control panel will display the RCF calculated for the r ax of the 1 8 or 1 5 mL tube which will not accurately represent the RCF for shorter tubes Refer to the rotor manual for the RCF values of shorter tubes Press the RPM key The last digit on the SPEED display 0 will flash indicating that the rpm can be entered in 100 rpm increments with the cursor keys Press the A or Y cursor key until the required rpm is displayed The corresponding RCF will be automatically calculated by the centri fuge but the rpm value will be displayed during the run You can check the RCF during the run by pressing the RCF key while the centrifuge is running Press the RCF key The last digit on the SPEED display 0 will flash indicating that the RCF can be entered 2 Press the A or Y cursor key u
13. 02009 Beckman Coulter Inc All rights reserved
14. Kid a ee Bow 5 3 Tube Breakage san aa ern ale ent 5 3 Decontamination ss tac sate ias Baw dae 5 4 Sterilization and Disinfection 0 0 eee e eee eee 5 4 Storage and Transport 0 0 ccc cee eee 5 4 SOTA TE sats dt da adas 5 4 Returning a Centrifuge o ooooooooorororrrrrrao o 5 4 Supplies 5 5 Replacement Parts 0 2 0 cece eee eens 5 5 SUPPHESH uasin ci A oaks ath a Ane BE at 5 5 PROGRAM LIBRARY Illustrations Page Figure 1 1 The Allegra 21 and Allegra 21R Centrifuges 22 2 1 2 Figure 1 2 Interior View of the Rotor Chamber 02 02 00 eae 1 4 Figure 1 3 The Power Switch 20 2 0 eee ro 1 6 Fig re l 4 The Control Panels ressens renan pies ma a 1 6 Figure 2 1 Dimensions of the Nonrefrigerated and Refrigerated Centri Uges He se in le Wha ick eR eae dea eee 2 3 Figure 3 1 Tapered Sleeve Position 0 0 0 cece eee eee eens 3 3 Figure 3 2 Installing a Rotor 2 0 0 cee cee nes 3 4 Figure 3 3 Default Parameters 0 ec ec c eee e ees 3 5 Figure 4 1 Emergency Door Release 0 0 0 0 cece cece 4 5 Figure 5 1 Replacing the Rotor Chamber Gasket 00200 00205 5 2 Tables Page Table 3 1 Acceleration Deceleration Times in minutes seconds 3 9 Table 4 1 Error Message Chart sun Masken Sd tae a eds 4 2 Table 4 2 Troubleshooting Chart unauna cece cece eee 4 4 CERTIFICATION SC
15. N IMBALANCE DOOR Digital displays indicate rotor speed run time rotor chamber temperature and numbers that represent selected acceleration and deceleration profiles When the power is turned on they show the operating parameters of the most recent run performed before the power was turned off The displays serve a dual purpose When the run parameters are being set the input mode the displays show the set values those selected by the operator When a run parameter key for example TIME or RPM is pressed the appropriate display flashes to indicate that data can be entered The actual real time operating conditions of the centrifuge are displayed during the run after START is pressed I gt NOTE Error messages see Section 4 also appear on the displays when applicable e In input mode the SPEED display shows the value of the parameter being set depending on the programming key pressed RPM RCF or ROTOR For example if the ROTOR programming key is pressed a rotor number appears on the SPEED display During centrifugation the SPEED display shows the speed of the rotor in rpm If the RCF key is pressed while the centrifuge is running the RCF value is displayed TIME TEMP C refrigerated models only ACC DEC Description e During a timed run between 1 minute and 9 hours 59 minutes the TIME display begins counting down when the rotor starts to spin and continues the countd
16. OPE OF MANUAL Introduction To ensure full system quality Beckman Coulter Allegra 21 series GS 15 series and Spinchron 15 series centrifuges are manufactured in a registered ISO 9001 or 13485 facility They have been designed and tested to be compliant when used with Beckman rotors with the laboratory equipment requirements of applicable regulatory agencies Declarations of conformity and certificates of compliance are available at www beckmancoulter com International symbols that may be displayed on the centrifuge are illustrated and described on the reverse side of the cover page This manual is designed to familiarize you with the Beckman Coulter Allegra 21 series GS 15 series and Spinchron 15 series benchtop centri fuges their functions specifications operation and routine operator care and maintenance The following introductory pages contain the instrument specifications as well as space electrical and temperature conditions required for optimal centrifuge performance A list of available rotors is also included e Section 1 provides a brief physical and functional description of the centrifuge and the operating controls and indicators e Section 2 contains instructions for installing and connecting the centrifuge Procedures for operating the centrifuge are summarized in Section 3 vii Introduction Vili CONVENTIONS NOTES CAUTIONS AND WARNINGS e Section 4 lists possible error messages and or
17. The control panel shown is a refrigerated model default values are the same for the nonrefrigerated model except there is no temperature indication 3 5 Operation 3 6 Selecting a Rotor Number Setting Run Speed When run parameters for a rotor have been entered as described below they will be retained in the centrifuge memory and can be recalled by simply entering the rotor number The recalled program can then be used for the current run or can be altered as required Each rotor that can be run in the centrifuge has a rotor number the number is engraved on the rotor and on the lid of fixed angle rotors The centri fuge internal memory contains a list of all rotors that can be run in the centrifuge together with acceptable run parameters for each rotor If you enter an unauthorized rotor number then press the START key an error message will be displayed and the run will be aborted shortly after the rotor starts spinning 1 Press the ROTOR key A rotor number the last rotor previously centrifuged will appear on the SPEED display 2 Press the A or Y cursor key until the number of the rotor in use is displayed 3 Press ENTER Nominal parameter values time temperature speed acceleration and deceleration curves for the selected rotor will be displayed You can use these parameters or set new values for the run Centrifuge speed can be set for up to the maximum rated speed of the selected rotor Either
18. a Normally only nonconductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected xi Introduction REFRIGERATED MODEL SPECIFICATIONS Only values with tolerances or limits are guaranteed data Values without tolerances are informative data without guarantee Speed Set speed ss Are 0 to 15 300 rpm in 100 rpm increments or equivalent RCF Time Set time Rn ee to 9 hr 59 min or continuous Time display 00000 c ee eee time remaining in run timed run or and elapsed time continuous run Temperature Temperature setting 20 to 40 C in 1 C increments Operating range 0 ec cece eee ene 2 to 40 CF Ambient temperature range 2 0 cece eee eee 10 to 30 C Humidity restrictions 22222222 c eee eee ee lt 95 noncondensing Acceleration b oid ena eae he is 10 acceleration profiles DeCelerations tc Solio wh AATE ti 10 deceleration profiles Dimensions Wb reais Hear nern 42 cm 16 6 in Depth a 2er Be ee ee a E 64 cm 25 2 in Height door closed 2 0 0 c ccc eee eee 38 cm 15 0 in Height door open eee eee 80 cm 31 5 in Weicht era een nee 70 kg 154 4 Ib Clearances sides and rear 2222202 c eee 7 6 cm 3 0 in Electrical requirements 50 Hz 230 VAC 5 A 60 Hz 120 VAC 12 A 50 60 Hz 100 VAC 15 A Motor ee en en er ren St a 746 W Electrical supply 0 0 0 eee ee ce
19. adiness The LEDs also blink if an incorrect parameter is entered o o o RPM RCF ROTOR o e OPEN IMBALANCE DOOR The cursor keys are up and down arrow keys A and W which can be pressed to increment values up or down when setting parameters Parameter speed time temperature and acceleration or deceleration curve changes made while a run is in progress must be verified by pressing the ENTER key When the RPM key is pressed the last digit in the SPEED display 0 flashes indicating that the speed can be entered in increments of 100 revolutions per minute rpm After the run starts the actual rpm of the rotor is displayed The RcF key can be used to select the speed setting by desired relative centrifugal field RCF The corresponding rpm is automatically calcu lated and displayed during the run If the RcF key is pressed during the run the RCF value is shown in the SPEED display ROTOR TIME TEMP refrigerated models only ACCEL DECEL Description The centrifuge memory contains a list of the rotors that can be used together with default parameters for each rotor When the ROTOR key is pressed the number of the rotor used in the previous run is shown on the SPEED display The rotor list can be scrolled through using the cursor keys until the required rotor number appears The TIME key is used to select the run duration When the TIME key is pressed the last digit
20. amber bowl be careful when examining or cleaning the gasket or chamber as sharp glass fragments may be embedded in their surfaces NEVER attempt to override the door interlock system while the rotor is spinning e Maintain a 7 6 cm 3 in clearance envelope around the centrifuge secured with antirotation kit while it is running During operation you should come within the envelope only to adjust instrument controls if necessary Never bring any flammable substances within the 30 cm 1 ft area surrounding the centrifuge Never lean on the centrifuge or place items on the centrifuge while it is operating Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic toxic or radioactive Such materials should not be used in this instrument however unless all necessary safety precautions are taken e Observe all cautionary information printed on the original solution containers prior to their use e Because spills may generate aerosols observe proper safety precautions for aerosol containment e Handle all infectious samples according to good laboratory practices and methods to prevent the spread of disease Ask your laboratory safety officer to advise you about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow if fluids escape from containers Biosafe containment should be used when Risk Group II m
21. aterials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Because spills may generate aerosols observe proper safety precautions for aerosol containment e Other infectious samples must also be handled according to good laboratory procedures and methods to prevent spread of disease e Dispose of all waste solutions according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the instrument and accessories before requesting service by our Field Service representative GS15 IM 8 April 2009 Allegra 21 Series GS 15 Series and Spinchron 15 Series Centrifuges Instruction Manual 2009 Beckman Coulter Inc Contents Page INTRODUCTION Gernifcation AN oe th neh vil Scope of Manual ii A ake aha a eB BOA vil CONVENTIONS fo lara rare RE ie RO ee MERA eG BEE Vili CFC Free Centrifugation 0000 cen x Radio Interference sm ooet rA n cette x Recycling Label satia eaer le re Geese x Nonrefrigerated Model Specifications 2 22222222 xi Refrigerated Model Specifications 0 00 nnana xii Available Rotors Social rin t EALA E aca xiii SECTION 1 DESCRIPTION Instrument Function and Safety Features 2 0 4 1 1 Instrument Function 0 0 cee cece eens 1 1 Models re ls ds 1 2 Safety Ec iii is dt a hohner 1 3 Name Rating Plate
22. by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed at the back of this manual for information about ordering parts and supplies For your convenience a partial list is given below Power cord 60 HZ u een ne 889097 Power cord 50 HZ o oe eA e nee 361130 Rotor chamber gasket ooooooooooororrrrrr 961806 Rotor tie down Screw erans ec eee ete e tenes 361367 T h ndle wrenich u 0 0 28 a nn ia fen ae 361371 Spinkote lubricant 2 oZ 2 6 ett 306812 Silicone vacuum grease 1 OZ n on nunnana eaa 335148 Beckman Solution 555 1 qt eect eens 339555 5 5 Appendix Program Library Record the program parameters on the chart below as a quick reference when duplicating run conditions Refer to Section 3 of this manual for the numbers representing acceleration and deceleration ACCEL DECEL rates Speed Program Time Temp ACCEL DECEL Number User Date Purpose RPM RCF hrs min C Rate Rate Program Library A 2 Program Number User Date Purpose Speed RPM RCF Time hrs min Temp C ACCEL Rate DECEL Rate ALLEGRA 64R AVANTI 30 GS 15 SERIES
23. ce envelope around the centrifuge secured with the anti rota tion kit while it is running No persons should be within this clearance boundary while the centri fuge is operating Do not handle or store hazard ous materials within the 30 cm 1 ft area surrounding the centrifuge 2 1 Installation 2 2 Stabilizer Tie down Bolt Position the centrifuge on a level surface such as a sturdy table or labora tory bench that is able to support the weight of the centrifuge refer to SPECIFICATIONS and resist vibration e Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation e Check that there are 7 6 cm 3 in clearances at the sides and back of the centrifuge to ensure sufficient air circulation Dimensions are shown in Figure 2 1 Additional clearance is required on the left side to allow access to the power switch The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation Ambient temperatures during operation should not be lower than 10 C 50 F or higher than 35 C 95 F Relative humidity should not exceed 95 noncondensing e Use the anti rotation kit to secure the centrifuge to the bench or table as described in publication GS TB 016 I gt NOTE During transport between areas with varying temper atures condensation may occur inside the centrifuge Allow sufficient drying time before running
24. cial attention to the safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the instrument Other symbols may also be displayed on the equipment These are reproduced and described on the reverse side of the cover page Safety During Installation and or Maintenance These centrifuges each weigh 50 kg 110 3 Ib nonrefrigerated model or 70 kg 154 4 Ib refrigerated model DO NOT attempt to lift or move one of them without assistance from another person Be sure to use the anchoring system to secure the centrifuge in place The anchoring system is designed to reduce the possibility of injury or damage which could result from instrument movement in the event of a major rotor mishap Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is turned off and the instrument is disconnected from the main power source and refer such servicing to qualified personnel Do not replace any centrifuge components with parts not specified for use on this instrument Electrical Safety To reduce the risk of electrical shock this equipment uses a three wire electrical cord and plug to connect this equipment to earth ground To preserve this safety feature e Make sure that the matching wall outlet receptacle is properly wired and earth grou
25. e run to begin This key can also be used to abort a deceleration process and restart the centrifuge The STOP key can be pressed to end a run The centrifuge decelerates to a complete stop according to the preselected deceleration curve Decelera tion can be terminated and the centrifuge restarted by pressing START again Pressing the FAST STOP key causes the centrifuge to decelerate to a complete stop at the maximum rate The deceleration cannot be inter rupted the centrifuge can only be restarted after the rotor stops and the door is opened and closed Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door to be opened The centrifuge will accept this command only when the rotor is completely stopped and the OPEN DOOR key LED is lit Pressing the PULSE key causes the installed rotor to accelerate up to the set speed for short duration runs as long as the key is pressed Decelera tion at the maximum rate begins when the key is released 1 7 Description Program Keys 4 CY Cursor Keys ENTER RPM RCF 1 8 The program keys are used to set run parameters a program consists of all of the parameters for a run Except for the cursor and ENTER keys program keys are located beneath the applicable digital displays which show the parameters as they are input Each key except for the cursor keys has an LED in the upper left corner that lights to indicate operational re
26. ed Angle GS TB 009 25 Angle C1015 Conical 9 500 9 380 9 000 8 420 10 x 15 364680 Fixed Angle GS TB 011 25 Angle S4180 5 500 5 450 4 500 3 650 4 x 180 361109 llo Li Swinging Bucket GS TB 004 Four buckets 2096 3 000 1 100 3 000 1 100 96 x 1 5 1 8 361111 Microtiter GS TB 005 Qs N H6002 12 200 12 400 12 200 12 400 60 x 1 5 1 8 363000 BioSafe 72 x 600 uL GS30 TB 002 Bowl Rotor 84 x 250 uL Values are rounded off xiii 1 Description This section provides a brief physical and functional description of the Beckman Coulter Allegra 21 series GS 15 series and Spinchron 15 series centrifuges The operating controls and indicators are also described instructions for their use are in Section 3 Chemical compati bilities of materials listed in this manual can be found in Chemical Resistances publication IN 175 Refer to the applicable rotor manuals for rotor descriptions INSTRUMENT FUNCTION AND SAFETY FEATURES INSTRUMENT FUNCTION The Beckman Coulter Allegra 21 series GS 15 series and Spinchron 15 series compact benchtop centrifuges generate centrifugal forces required for a wide variety of applications Together with any of several Beckman Coulter rotors designed specifically for use in these centrifuges the instru ment applications include e Routine processing such as sample preparations pelleting extractions purifications concentrations phase separations recep
27. es to a complete stop open and close the centrifuge door then restart 4 Drive error mechanical 4 Call Beckman Coulter Field Service damage Rotor cannot 1 Line voltage below rating 1 Have a qualified service person measure line achieve set speed voltage while the instrument is operating 2 Electrical failure 2 Make sure both ends of the power cord are securely connected call Beckman Coulter Field Service 3 Motor failure 3 Call Beckman Coulter Field Service Door will not open 1 Rotor spinning 1 Wait until the rotor stops 2 Power not on 2 Plug in the power cord turn power on I 3 Source power failure 3 See EMERGENCY ACCESS below 4 Latch stuck 4 See EMERGENCY ACCESS below Displays are blank 1 Power not on 1 Plug in the power cord turn power on I 2 Electrical failure 2 Make sure both ends of the power cord are securely connected call Beckman Coulter Field 3 Fuse blown Service 3 Call Beckman Coulter Field Service TEMP C display flashes refrigerated models Chamber temperature is gt 25 C higher than selected temperature Precool rotors before running at low temperatures Precool rotor chamber by running a 30 minute cycle at the desired temperature with the speed set at about 2000 rpm If a lower temperature deviation alert is required contact Beckman Coulter Field Service Chamber does not reach selected temperature refrigerated models Centrifuge cannot mainta
28. he door 9 Unload the rotor according to instructions in the applicable rotor manual If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Appropriate decontamination procedures should be applied to the centrifuge and accessories USER MESSAGES 4 Troubleshooting This section lists possible malfunctions together with probable causes and corrective actions required Maintenance procedures are contained in Section 5 For any problems not covered here contact your Beckman Coulter Field Service representative gt NOTE It is your responsibility to decontaminate the centrifuge as well as any rotors and accessories before requesting service by Beckman Coulter Field Service representatives If the message SEr appears on the display do not press any keys while the message is displayed Turn the centrifuge power off O and back on 1 to clear the message This message indicates that you have inadvertently accessed the service mode Pressing any keys while in this mode could erase the centri fuge memory and critically interfere with future operation If a problem occurs during operation the rotor will decelerate to a stop and an error code will appear on the SPEED display Messages may result from incorrect input or from an equipment malfunction Refer to Table 4 1 4 1 Troubleshooting Table 4 1 Error Message Chart If the recommended action does not correct the problem cal
29. in selected temperature for rotor in use at speed selected Refer to appropriate rotor manual for temperature and speed requirements Also precool rotors before running at low temperatures Precool rotor chamber by running a 30 minute cycle at the desired tempera ture with the speed set at 2000 rpm Troubleshooting m 2 1 Centrifuge Figure 4 1 Emergency Door Release 2 Make sure that the rotor is not spinning 3 Insert a socket head wrench in the emergency door release screw on the centrifuge side panel see Figure 4 1 4 Rotate the screw clockwise until you hear a click or the door pops open 5 Remove the wrench If the rotor is still spinning close the door and wait until it stops before attempting to remove it A WARNING Never try to slow or stop the rotor by hand 4 5 5 Care and Maintenance For maintenance not covered in this manual contact your local Beckman Coulter Field Service representative User messages are discussed in Section 4 TROUBLESHOOTING Refer to the applicable rotor manual and to Chemical Resistances publication IN 175 for instructions on the care of rotors and their accessories gt NOTE It is your responsibility to decontaminate the centrifuge as well as any rotors and accessories before requesting service by Beckman Coulter Field Service representatives A WARNING Any maintena
30. is in place at the base of the centri fuge drive shaft before installing the rotor see Figure 3 1 The rotor rests on the sleeve while spinning and will not operate properly if the sleeve is missing Wipe the sleeve to be sure that it is clean and dry Make sure tapered sleeve is in place before installing rotor Figure 3 1 Tapered Sleeve Position A CAUTION Do not drop the rotor onto the drive shaft The shaft can be damaged if the rotor is forced sideways or dropped onto it Install the rotor by centering it over the shaft and carefully lowering it straight down 6 Install the rotor see Figure 3 2 according to the instructions in the appropriate rotor manual Always run the rotor with a balanced load If you are using a horizontal swinging bucket rotor fill all four positions on the yoke of a swinging bucket rotor with buckets 7 Attach the tie down screw to the drive shaft by turning it to the right clockwise 8 Use the T handle wrench to tighten the tie down screw on the shaft If the rotor is left in the centrifuge between runs make sure the rotor is seated on the drive shaft and the tie down screw is tight before each run Remove the rotor from the centrifuge if a long period between runs is anticipated 3 3 Operation SWINGING BUCKET ROTOR FIXED ANGLE ROTOR Lower the rotor straight down onto the drive shaft Lower the yoke straight down onto the drive shaft Tighten the tie dow
31. l Beckman Coulter Field Service Error Number Problem Result Recommended Action 1 through 23 25 and 26 28 through 30 32 36 39 and 40 44 through 60 63 through 68 Microprocessor or mechanical malfunction Deceleration to full stop run cannot be restarted After rotor comes to a complete stop turn the power off O then back on I to reset 23 and 24 27 31 33 through 35 37 and 38 41 through 43 62 Microprocessor or mechanical malfunction Deceleration to full stop run cannot be restarted After rotor comes to a complete stop open and close the centrifuge door then restart 69 through 77 Microprocessor malfunction Run cannot start until error is cleared Turn the power off O then back on I to reset 78 through 80 Error during closing of door Run cannot start 1 Remove debris in latch 2 Close lid quickly 3 Turn the power off O then back on I to reset 81 Door open Maximum deceleration After rotor comes to a complete stop shut detected during run to full stop the centrifuge door turn the power off O then back on I to reset 82 83 Door does not open See EMERGENCY ACCESS below 84 Heat sink Deceleration to full stop 1 Check that ambient temperature is overtemperature within a the limits shown in SPECIFICATIONS 85 Rotor chamber Deceleration to full stop 5 Chekar inet and exhausts for through overtemperature
32. l switch off if the chamber temperature reaches 65 C Restarting the centrifuge will not be possible until the chamber is cooled CONTROLS AND INDICATORS POWER SWITCH The power switch is located on the centrifuge back panel see Figure 1 3 This two position rocker switch I on O off controls electrical power to the centrifuge I gt NOTE The power must be turned on before the chamber door can be opened 1 5 Description 1 6 CONTROL PANEL I Power Switch y Figure 1 3 The Power Switch The control panel Figure 1 4 mounted at an angle on the centrifuge front for easy visibility and access comprises touch keys system keys and programming keys and digital displays The panel also contains an IMBALANCE light that flashes if rotor loads are severely out of balance o e OPEN FAST g IMBALANCE DOOR STOP STOP START TEMP C ACC DEC OPEN IMBALANCE DOOR Allegra 21R GS 15R or Spinchron 15R Figure 1 4 The Control Panels System Keys START STOP FAST STOP OPEN DOOR PULSE Description The centrifuge operation is controlled through the system keys Each key except the PULSE key has an LED in the upper left corner that lights to indicate that the key can be activated o a e RPM RCF ROTOR o e OPEN IMBALANCE DOOR Pressing the START key causes the centrifug
33. malfunctions together with probable causes and corrective actions required Procedures for operator care and maintenance are presented in Section 5 as well as a brief list of supplies and replacement parts A Program Library chart is provided at the back of this manual You can use this chart to record the parameters of a run for later duplication of the run conditions We recommend that you read this entire manual especially the SAFETY NOTICE and all safety related information before operating the centrifuge or performing instrument maintenance gt NOTE If the centrifuge is used in a manner other than spec ified in this manual the safety and performance of this equipment could be impaired Further the use of any equipment other than that intended for use by Beckman Coulter has not been evaluated for safety Use of any equipment not specifically recommended in this manual is the sole responsibility of the user Certain symbols are used in the manual to call out safety related and other important information These are reproduced and described below and or on the inside of the front cover I gt NOTE Used to call attention to information that should be followed during installation use and or servicing of the equipment TYPOGRAPHIC CONVENTIONS Introduction A CAUTION Used to indicate a potential hazardous situation which if not avoided may result in minor or moderate injury and or mechanical damage It is
34. ment 3 1 Operation A WARNING The centrifuge must not be used in the vicinity of flammable liquids or vapors and such materials should not be run in the centrifuge During oper ation you should come within the 7 6 cm 3 in clearance envelope only to adjust instrument controls if necessary Never bring any flammable substances within the 30 cm 1 ft area surround ing the centrifuge Do not lean on the centrifuge or place items on the centrifuge while it is operating RUN PROCEDURE The following detailed operating procedures are summarized atthe end of this section If you are an experienced user of this centrifuge you can turn to the summary for a quick review of operating steps PREPARATION For fast temperature equilibration cool or warm the rotor to the required AND LOADING temperature before the run I gt NOTE For high speed runs at temperatures of 20 C or higher prime the refrigeration system refrigerated models by running the instrument at 10 C for 5 to 10 minutes beforehand to prevent overheating 1 Check the name rating plate for the correct voltage then plug the power cord into the wall receptacle 2 Press the power switch to on I 3 Press the OPEN DOOR key and lift the door up it will remain in the open position 4 Use the T handle wrench to turn the rotor tie down screw to the left counterclockwise Remove the tie down screw 3 2 Operation 5 Make sure that the tapered sleeve
35. n screw clockwise onto the drive shaft Tighten the tie down screw clockwise onto the drive shaft Seat filled buckets on the yoke pins Attach the lid and tighten with the T handle wrench Figure 3 2 Installing a Rotor 3 4 ENTERING RUN PARAMETERS Operation 9 If using a fixed angle rotor with a lid attach the lid Tighten the lid with the T handle wrench gt NOTE Fixed angle rotors can be centrifuged without lids attached when longer tubes are used 10 Close the centrifuge door and push firmly down on both sides of the door front until you hear a clicking latching sound When the power is applied for the initial use no previous runs default values will be displayed see Figure 3 3 The centrifuge has an internal memory that stores previously used run parameters or programs for each rotor that has been centrifuged After the initial use the program parame ters of the latest previous run will be displayed when power is applied I gt NOTE A Program Library is provided at the back of this manual You can use this chart to record the parameters of a run for later duplication of the run conditions The centrifuge The chamber The will accelerate The run duration will be cooled acceleration to 2000 rpm will be 2 minutes to 20 C curve is 8 TEMP C o o o o o e RPM RCF ROTOR TIME ACCEL ENTER o gt OPEN o IMBALANCE DOOR STOP START Figure 3 3 Default Parameters
36. nce procedure or servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is off O and the centrifuge is disconnected from the main power source and refer such servicing to qualified service personnel Do not use alcohol or other flammable substances in or near operating centrifuges 5 1 Care and Maintenance MAINTENANCE PREVENTIVE MAINTENANCE REPLACING THE ROTOR CHAMBER GASKET 5 2 The following procedures should be performed regularly to ensure continued performance and long service life of the centrifuge Regularly inspect the interior of the rotor chamber for accumulations of sample dust or glass particles from broken sample tubes Clean as required see CLEANING below as these accumulations can result in rotor vibrations Regularly check the air intake and exhaust vents for obstructions Keep vents clear and clean Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber icing refrigerated models If chamber icing occurs defrost before use To prevent the rotor from sticking lubricate the drive shaft with Spinkote at least once a month and after each cleaning If the gasket 961806 around the rotor chamber opening becomes damaged or worn replace it as follows See Figure 5 1 1 2 Remove the gasket by pulling it up and away from the opening
37. nded Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the instrument e Never use a three to two wire plug adapter e Never use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip Do not place containers holding liquid on or near the chamber door If they spill liquid may get into the instrument and damage electrical or mechanical components Safety Against Risk of Fire Certain electrical circuits within this equipment are protected by fuses against overcurrent conditions For continued protection against the risk of fire replace only with the same type and rating specified This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials chloroform or ethyl alcohol for example in this instrument nor handle or store them within the 30 cm 1 ft clearance envelope surrounding the centrifuge Mechanical Safety For safe operation of the equipment observe the following Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge Before starting the centrifuge make sure that the rotor tie down screw is securely fastened Do not exceed the maximum rated speed of the rotor in use NEVER attempt to slow or stop the rotor by hand Do not lift or move the centrifuge while the rotor is turning e Ifa glass tube breaks inside the ch
38. ng container to protect it from dust and dirt Reinstall the plastic stabilizer removed at time of centrifuge installation to prevent damage to the drive rotor and imbalance detector Temperature and humidity condi tions for storage should meet the environmental requirements described under SPECIFICATIONS Before returning a centrifuge or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions Flammability hazard Do not use in or near operating centrifuges SUPPLY LIST REPLACEMENT PARTS SUPPLIES Care and Maintenance To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts All parts must be accompanied by a signed note plainly visible on the outside of the box stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Refer to the applicable rotor manual for materials and supplies needed for rotors gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or
39. nment This equip ment generates uses and can radiate radio frequency energy and if not installed and used in accordance with this instruction manual may cause interference to radio communications Operation of this equipment in a residential area may cause interference in which case the user will be required to correct the interference at his or her own expense This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regu lations of the Canadian Department of Communications Le pr sent appareil num rique n met pas de bruits radio lectriques d passant les limites applicables aux appareils num riques de Classe A prescrites dans le reglement sur le brouillage radioelectrique dict par le Minist re des Communications du Canada This symbol is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates 1 the device was put on the European market after August 13 2005 and 2 the device is not to be disposed via the municipal waste collection system of any member state of the European Union It is very important that customers understand and follow all laws regard ing the proper decontamination and safe disposal of electrical equipment For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulte
40. nstalled at the desired temperature with the speed set at 2000 rpm 1 Press the POWER switch to on I Open the centrifuge door press the OPEN DOOR key and lift the door up Make sure that the tapered sleeve is in place at the base of the centri fuge drive shaft before installing the rotor The rotor will not operate properly if the sleeve is missing Install the rotor according to the instructions in the applicable rotor manual Always run the rotor with a balanced load Close the centrifuge door and push firmly down on it until you hear the latch engage Enter run parameters Select a rotor number ROTOR A or V ENTER Set run speed RPM A or V or RCF A or Y e Set run duration TIME A or Y e Set run temperature TEMP A or Y e Select acceleration rate 0 through 9 ACCEL A or Y e Select deceleration rate 0 through 9 DECEL A or Y Operation 6 Check that all parameters are correct and the door is shut and latched then press ENTER then START A WARNING Never attempt to override the door interlock sys tem while the rotor is spinning A CAUTION Do not lift or move the centrifuge while the rotor is spinning 7 Wait for the set time to count down to zero or end the run by pressing either the STOP key or the FAST STOP key 8 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the door latch open t
41. nt eee e ee neees Class I Maximum heat dissipation into room under steady state conditions 04 2546 Btu h 0 75 kW Noise level 0 91 m 3 ft in front of instrument lt 64 dBa Installation overvoltage category 0 eect eee TI Pollution degree dd do 2t Temperature range depends on rotor in use and speed see applicable rotor manual Normally only nonconductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected xii Introduction AVAILABLE ROTORS Refrigerated Nonrefrigerated Max Max Rotor Profile and Max RCF Max Max RCF Capacity Rotor Part Number Description RPM x g RPM x g mL Rotor Manual Number F2402H 15 300 21 460 14 500 19 280 24 x 1 0 1 5 361171 Fixed Angle GS TB 021 45 Angle F3602 14 000 19 280 12 500 15 370 36 x 2 0 1 5 364600 Fixed Angle GS TB 006 45 Angle F0630 15 300 20 410 14 500 18 340 6 x 30 361231 Fixed Angle GS TB 014 al 30 Angle F0850 10 000 10 730 9000 8 690 8x50 346640 Fixed Angle GS TB 003 25 Angle F0685 8 000 7 010 8 000 7 010 6 x 85 364650 Fixed Angle GS TB 008 25 Angle F1010 15 300 19 890 14 500 17 870 10 x 10 361221 Fixed Angle GS TB 007 35 Angle C0650 Conical 9 500 9 380 9 000 8 420 6 x 50 364670 Fix
42. ntil the required RCF is displayed The corresponding rpm will be automatically calculated and the centrifuge will run at the calculated speed The rpm value will be displayed during the run You can check the RCF during the run by pressing the RcF key while the centrifuge is running 3 7 Operation 3 8 Setting Run Time Run time can be set for either a timed run or continuous operation e Timed run Time can be set for up to 9 hours and 59 minutes if the minutes parameter entered exceeds 59 it is automatically converted into hours During centrifugation the TIME display begins counting down when the rotor starts to spin and continues the count down until deceleration begins The TIME display shows the time remaining in the run in hours and minutes When the time display reaches zero the run ends Continuous run If a run time of less than 0 or more than 9 hours and 59 minutes is selected continuous operation is activated Time is not counted down during continuous operation instead the infinity co symbol indicating continuous operation lights up and time elapsed since the run start is displayed The run will continue until the STOP or FAST STOP key is pressed Press the TIME key The last digit on the TIME display will flash indicating that the time can be entered with the cursor keys 2 Press the A or Y cursor key until desired run duration is displayed Setting Run Temperature Run temperature
43. obable causes and corrective actions required Possible causes for each problem are listed in the probable order of occurrence Perform the recommended corrective action in sequence as listed If you are unable to correct the problem call your Beckman Coulter Field Service representative EMERGENCY ACCESS If the facility power fails only momentarily the centrifuge will resume operation when power is restored and the rotor will return to set speed However if the rotor comes to a complete stop you will have to restart the run when the power is restored In the event of an extended power failure it may be necessary to trip the door locking mechanism manually to remove the rotor and retrieve your sample see Figure 4 1 1 Turn the power switch to off O and disconnect the power cord from the main power source A WARNING Never attempt to override the door interlock system while the rotor is spinning 4 3 Troubleshooting Table 4 2 Troubleshooting Chart Problem Problem Result Recommended Action Imbalance LED lights and rotor decelerates to stop Rotor is out of balance Centrifuge is misaligned tilted Centrifuge was moved during operation 1 Check to be sure the rotor is in good condition and is loaded symmetrically around the center of rotation with containers of equal weight and density within 6 grams opposite each other 2 Align the centrifuge on the bench or table 3 After the rotor com
44. on the TIME display flashes indicating that the time can be entered with the cursor keys e Timed run Run time up to 9 hours and 59 minutes can be set If the minutes parameter exceeds 59 it is automatically converted into hours e Continuous run If a run time of less than 0 or more than 9 hours and 59 minutes is selected continuous operation is activated Time is not counted down and the run will continue until the STOP or FAST STOP key is pressed The TEMP key is used to select run temperature on refrigerated model centrifuges When the TEMP key is pressed the TEMP C display flashes indicating that the temperature can be entered with the cursor keys Temperature can be set between 20 and 40 C The ACCEL key is used to select acceleration rates that will protect delicate gradients When the ACCEL key is pressed the ACC DEC display flashes indicating that one of ten preset rates can be entered with the cursor keys Acceleration rates are described in Table 3 1 in Section 3 The DECEL key is used to select deceleration rates that will maintain optimum separation while protecting delicate gradients When the DECEL key is pressed the ACC DEC display flashes indicating that one of ten preset rates can be entered with the cursor keys Deceleration rate selec tions are described in Table 3 1 in Section 3 1 9 Description Digital Displays SPEED e e o RPM RCF ROTOR gt OPE
45. own until deceleration begins The TIME display indicates the remaining run time in hours and minutes e During a continuous run less than 0 or more than 9 hours 59 minutes selected countdown time is not displayed Instead the infinity 00 symbol indicating continuous operation lights up and the TIME display shows time elapsed since the run start After 9 hours and 59 minutes the timer will reset to O and continue counting elapsed time During standby that is the centrifuge is turned on but not spinning and operation the TEMP C display shows the actual temperature inside the rotor chamber 2 C at an ambient temperature of 20 C The ACC DEC display shows the acceleration rate that was selected for the run The deceleration curve number can be displayed by pressing the DECEL key 2 Installation This section contains instructions for installing and connecting the centri fuge Check that required clearances and electrical power are available A WARNING These centrifuges each weigh 50 kg 110 3 Ib nonrefrigerated model or 70 kg 154 4 Ib refrigerated model DO NOT attempt to lift or move one of them without assistance from another person INSTALLING THE INSTRUMENT A WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids Vapors from these materials could enter the centrifuge air system and be ignited by the motor A WARNING Maintain a 7 6 cm 3 in clearan
46. place The anchoring system is designed to reduce the possibility of injury or damage which could result from instrument movement in the event of a major disruption The name rating plate is affixed to the rear of the centrifuge Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge The centrifuge housing is made of sheet steel finished with urethane paint The control panel is covered by a protective overlay made of coated polycarbonate 1 3 Description 1 4 DOOR ROTOR CHAMBER DRIVE The solid sheet aluminum door is secured to the housing by solid shafts A dual electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and latched The door is locked when a run is in progress and can be opened only when the rotor is stopped A light emitting diode LED on the OPEN DOOR key lights up when the door can be opened In the event of a power failure the door lock can be manually tripped for sample recovery see Section 4 TROUBLESHOOTING The rotor chamber is shown in Figure 1 2 The drive shaft mounting plate rubber boot surrounding the drive shaft thermistor and rotor detector are visible in the chamber bottom A gasket system around the chamber opening assure
47. r office for details on the take back program that will facilitate the proper collection treatment recovery recycling and safe disposal of the device Introduction NONREFRIGERATED MODEL SPECIFICATIONS Only values with tolerances or limits are guaranteed data Values without tolerances are informative data without guarantee Speed Set speed O ee tasty 0 to 14 500 rpm in 100 rpm increments or equivalent RCF Time Set Imre to 9 hr 59 min or continuous Time display 0 0 00 200 eee time remaining in run timed run or and elapsed time continuous run Acceleration Acct nn er re 10 acceleration profiles Deceleration A ae ea ia 10 deceleration profiles Ambient temperature range Humidity restrictions 2 2 22 222222 Dimensions Depth ii ici eek er ad 10 to 35 C Ls lt 95 noncondensing a Senet 42 cm 16 6 in FREE RER a 49 cm 19 3 in wht He Bea gees 38 cm 15 0 in nae anton ct Ades ooh N 80 cm 31 5 in BREUER DE eR 50 kg 110 3 Ib RS HERNE RR ORRI 7 6 cm 3 0 in Electrical requirements 50 Hz 230 VAC 3 A 60 Hz 120 VAC 5 A 50 60 Hz 100 VAC 15 A Motor une ee a a Me te SR ARES esto 340 W Electrical SUPRA IEA AA Class I Maximum heat dissipation into room under steady state conditions Noise level 0 91 m 3 ft in front of instrument Installation overvoltage category Pollution degree 00 002 ee eee e inci lt 64 dB
48. ress This display also shows the time remaining in the run or and elapsed time for continuous operation A WARNING Do not attempt to override the door interlock system while the rotor is spinning A CAUTION Do not lift or move the centrifuge while the rotor is spinning While a run is in progress run parameters speed time temperature and acceleration or deceleration rate can be altered without stopping the run Run duration can also be changed from continuous to a specified time period or from a specified time period to continuous I gt NOTE The deceleration rate cannot be changed after decel eration starts Use the program keys as described under ENTERING RUN PARAMETERS above to change parameters Parameter changes made during a run must be verified by pressing the ENTER key For example to change run speed during centrifugation 1 Press the RPM key The last digit on the SPEED display will flash indicating that the rpm can be raised or lowered with the cursor keys 2 Press the A or Y cursor key until the required rpm is displayed 3 Press the ENTER key The current rpm value will be displayed changing to the new value as the rotor accelerates or decelerates to the new speed selected The corresponding RCF will be automatically calculated by the centrifuge Operation STOPPING A RUN A timed run will end automatically when the TIME display counts down to zero To end a run in progress for any
49. rther information regarding new accessories and or modifications as they become available 3 Operation This section contains operating procedures for the centrifuge using any of the Beckman Coulter rotors designed for use in these centrifuges Refer to the appropriate rotor manual for instructions on preparing the rotor for centrifugation To prevent condensation keep the centrifuge door closed and the power turned off O when the centrifuge is not in use A WARNING Normal operation may involve the use of solu tions and test samples that are pathogenic toxic or radioactive Operator error or tube failure may generate aerosols Do not run potentially hazardous materials in this centrifuge unless all appropriate safety precautions are taken Always use the appropriate rotors and adapters Handle all infectious samples according to good laboratory practices and methods to prevent the spread of disease Ask your laboratory safety officer to advise you about the level of contain ment required for your application and about the proper decontamination or sterilization procedures to follow if fluids escape from containers Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Because spills may generate aerosols observe proper safety precautions for aerosol contain
50. s sealing Instrument gaskets have not been designed as bioseals for aerosol containment Thermistor Drive Shaft refrigerated models only Rotor Detector Tapered Sleeve Gasket a Boot Figure 1 2 Interior View of the Rotor Chamber The asynchronous three phase direct drive motor is brushless for clean quiet operation A tie down screw is used to attach the rotor to the drive shaft The resilient suspension ensures that loads will not be disturbed by vibration and prevents damage to the drive shaft if an imbalance occurs during centrifugation Maximum braking may be selected to reduce decel eration time allowing fast processing of samples alternately delicate gradients may be preserved using slower deceleration Description TEMPERATURE SENSING With the power on the temperature control system is activated when the AND CONTROL door is closed The run temperature can be set between 20 and 40 C in refrigerated models only refrigerated models If no set temperature is entered the centrifuge auto matically selects the last entered temperature For the first run of a new centrifuge the instrument selects 20 C as its operating temperature A thermistor in the rotor chamber continuously monitors chamber tempera ture The micro processor calculates the required chamber temperature to maintain the selected rotor temperature I gt NOTE In the unlikely event of a complete cooling system failure the drive wil
51. t a concern maximum brake can be used However if delicate gradients are being run a lower brake setting may be needed Deceleration rates are listed in Table 3 1 1 Press the DECEL key The ACC DEC display will flash indicating that the selected number can be entered with the cursor keys 2 Press the A or Y cursor key until the required number is displayed The run can be started using the parameters in memory from a previous run or using new or changed parameters that you enter using the procedure described above 1 Check that all parameters are correct and the door is shut and latched 2 Press the ENTER key then the START key As the run begins the instrument rotor identification system compares the rotor in the chamber to the entered rotor number and the speed entered An incorrect rotor identification or set speed greater than the rotor s maximum permitted speed will result in an error code and the centrifuge will shut down See Section 4 TROUBLESHOOTING for information on error codes The error must be cleared and an appro priate speed entered before the centrifuge can be started Throughout the run checks are made to ensure that the rotor does not exceed set speed e The SPEED display indicates the rotor speed in rpm The RCF can be checked by pressing the RCF key CHANGING PARAMETERS DURING A RUN Operation e A blinking LED at the bottom of the TIME display indicates that the run is in prog
52. the centrifuge The centrifuge is shipped with a cone shaped plastic stabilizer installed in the rotor chamber to prevent damage to the drive motor and imbalance detector during transit When installation of the centrifuge is complete unscrew the tie down bolt and remove the stabilizer Save the stabilizer to use during future relocation of the centrifuge ELECTRICAL REQUIREMENTS 100 V instrument 00 000 90 100 VAC 15 A 50 60 Hz 120 V instrument refrigerated 108 132 VAC 12 A 60 Hz 120 V instrument nonrefrigerated 108 132 VAC 5 A 60 Hz 230 V instrument refrigerated 207 253 VAC 5 A 50 Hz 230 V instrument nonrefrigerated 207 253 VAC 3 A 50 Hz Power cord 0 cece eee 1 8 m 6 ft power cord with grounded ios a oe nee aoe eee plug is supplied with the instrument Make sure the voltage and frequency imprinted on the name rating plate affixed to the back of the centrifuge agree with the line voltage and frequency of the outlet used Refrigeration will not function properly if the frequency Hz does not match the name rating plate Plug in both ends of the centrifuge power cord If there is any question about voltage have a qualified service person measure it under load while the drive is operating Installation 000 a Allegra 21 GS 15 or Spinchron 15 o 80 0 31 5 Allegra 21R aR centrifuge
53. tor binding and column centrifugations Processing large numbers of small volume samples in multiwell plates for concentrating tissue culture cells cloning and replicate studies in vitro cytoxicity studies receptor binding and genetic engineering experimentation e Rapid sedimentation of protein precipitates large particles and cell debris Binding studies and separation of whole blood e Cell isolation 1 1 Description 1 2 MODELS The centrifuges are microprocessor controlled providing interactive oper ation The instrument design features a brushless three phase drive system automatic rotor overspeed identification system program memory that can repeat the previously used run parameters for a given rotor and a choice of acceleration deceleration rates The refrigerated models also have temperature control systems User messages alert the operator to conditions that may need attention The centrifuge is available in both nonrefrigerated and refrigerated models See Figure 1 1 See the SPECIFICATIONS listed above for the operating differences between the refrigerated and nonrefrigerated models Unless indicated otherwise information in this manual is the same for both models Megra 21 Centrifuge Su N Figure 1 1 The Allegra 21 and Allegra 21R Centrifuges GS 15 GS 15R Spinchron 15 and Spinchron 15R models are similar in appearance SAFETY FEATURES NAME RATING PLATE CHASSIS HOUSING
54. trans portation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all trans portation charges Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give rea sonable assistance to the Buyer in obtaining from the respec tive manufacturer whatever adjustment is reasonable in light of the manufacturer s own warranty CONDITIONS Beckman Coulter shall be released from all obligations under all warranties either expressed or implied if the product s covered hereby are repaired or modified by persons other than its own authorized service personnel unless such repair in the sole opinion of Beckman Coulter is minor or unless such modification is merely the installation of a new Beckman Coulter plug in component for such product s DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT B BECKMAN COULTER Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com
55. ucket or rotor it will be necessary to thoroughly clean the interior of the chamber bowl A WARNING Be careful when examining or cleaning the sealing gasket or chamber as sharp glass frag ments may be embedded in their surfaces 5 3 Care and Maintenance 5 4 e Examine the gasket to make sure that no glass particles are retained in it Carefully remove any glass particles that may remain e Carefully wipe away any glass particles that remain in the bowl DECONTAMINATION If the instrument and or accessories are contaminated with radioactive or pathogenic solutions perform appropriate decontamination procedures Refer to Chemical Resistances IN 175 to be sure the decontamination method will not damage any part of the instrument STERILIZATION AND DISINFECTION The centrifuge is finished with urethane paint Ethanol 70 may be used on this surface See Chemical Resistances for more information regarding chemical resistance of centrifuge and accessory materials While Beckman Coulter has tested these methods and found that they do not damage the centrifuge no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use STORAGE AND TRANSPORT STORAGE Tie down Bolt Stabilizer RETURNING A CENTRIFUGE Before storing a centrifuge for an extended period return it to its original shippi

Download Pdf Manuals

image

Related Search

Related Contents

Châtel Saint  ID Stationライト 取扱説明書(PDF・約8MB)  Univex 95Ce User's Manual  2 Anschluss  Perfo Inox  Télécharger la documentation - videoprojecteur  Télécharger - Le français dans le monde    user manual  Naii”naー 施工説明書  

Copyright © All rights reserved.
Failed to retrieve file