MagCore® Viral Nucleic Acid Extraction Kit
Contents
1. to 60 C for Lysis Step While program finished a beep sound can be heard and green Indicate LCD light went out LCM displays as left picutre After clean up disposables you can choose to Start run the same protocol again Stop to Stand By menu for UV sterilization or power shut down UV Sterilization Turn on power switch located at the right hand MAGCORE HF16 side of MagCore HF16 A ee beep sound can be heard and the machine is on Stand By condition Press UV button to proceed a UV sterilization with 30 60 BUY Lamp Actived UV LIGHT a DO NOT OPEN DOOR lt 1 gt 1hr lt 2 gt 30min mins of time options N lt ESC gt To Back 059 Min 60 Min Door Open Interruption Emergent Stop If the front door was opened mechanical movements immediately due to safty consideration A a during program performing EMERGENT STOP MagCore HF 16 stops all I lt START gt CONTINUE PROGRAM lt STOP gt ABORT ee LCM display screen shows Stand By menu as left picture UV lamp actives and count down with selected time at minutes MagCore HF 16 will go back to Stand By menu once UV sterilization procedure completed LCM display screen shows warning sign Red Indicate LCD lights up and a beep sound can be heard Pull down the front door and select Start continue program Stop abort program and back to Stand By menu In case of eme2. 0 MagCore HF 16 Specification Model Processing Capacity Protocols V1 01 System Method Sample Volume Elution Volume Yield Purity Processing Time System Components Power Supply Dimension Net Weight HF16 16 samples per batch Programs built in Whole Blood 200 400ul Viral Nucleic Acid DNA RNA Tissue Genomic DNA Plant Genomic DNA Cellulose coated magnetic beads 200 400 1 200u 2 400ul available on request with optional 2 4ml Rack 60 100 150 200 ul Avrage 6ug Genomic DNA 200ul human whole blood O D 1 8 2 0 Ax60 rg0 ratio 30 70 minutes depends on sample type and method 1 Pipetting Unit Dispensing transferring X Z two axis movements 2 Electric Control Internal microprocessor 3 UV Light Power 5W life duration gt 1 000 Hrs 4 Heating Block RT 100 C 5 Display Screen 3 inches LCM Screen with keypress Panel 6 Accessories T Rack Cartridge Rack Empty Cartridges Tip Holders Microcentrifugr Tube Syringe O ring and Grease Voltage AC 100 240V Frequency 50 60Hz Power Consumption gt 1 0KVA W550 X D660 X H680 mm W21 65 X D25 98 X H26 77 inches 70kg 155lbs LCM Display Screen and Keypress Panel MagCore HF16 provides a touch button keypree panel for you to communicate with your instrument The number keys give users to input Catridge Code which specified on the Reagent Cartridge to run built in program automatically or to select desired volumes at start up sample and f
3. Free Water B Pipet 200 400ul serum sample to a microcentrifuge tube then add 5ul of Carrier RNA solution to per 200u of serum sample C Put the Sample Tube into hole 4 of T Rack Store the Carrier RNA at 4 C for long term storage 5 Operation Procedures Instal the Tip Holder and Sample Elution Tubes loaded T Rack into outter operation area of the MagCore HF 16 and lock firmly Pull down the front door to interlock position and then press Enter to next page for elution volume selection Select final elution volume MagCore HF 16 in process of ES ELUTE VOL y lt 1 gt 200ul ELUTE VOLUME 200pl selected protocol at this 1 gt 200 2 gt 150 oo a o lt 2 gt 150ul EEEE step The Green Indicat LCD RS i lt 3 gt 100ul Wns sels l lights up and Heating Block lt 4 gt 60l During MagCore HF16 is under program running the MagCore LCD lights up at all times DO NOT open the door at this moment it causes emergent stop and you might lose your samples by machine interruption Lift up the front door to the uppermost position and take out Elution Tube with purified viral DNA RNA direct for downstram applications in PCR and RT PCR or store at 20 C for long term stroage Discard all disposables under proper procedures PROCEDURE COMPLETED lt START gt CONTINUE lt STOP gt STAND BY Y E PROCEDURE COMPLETED lt START gt CONTINUE lt STOP gt STAND BY starts to heat up
4. User Manual MagCore Viral Nucleic Acid Extraction Kit For extracting Viral DNA RNA from serum plasma cell free body fluids by using the MagCore HF 16 System of RBC Bioscience Corp Cat No MVN400 01 MVN400 02 Cartridge Code 201 MAC1601 98001 MagCore HF16 Automated DNA RNA Purification System MagCore HF 16 System Reliable Nucleic Acid Purification MagCore HF16 System is a simple fast and cost effective technique to automatically purify nucleic acids from a diverse range of sample sources With the pre programmed protocols and magnetic bead based reagent cartridges the system provides consistent and stable nucleic acid purification for every busy laboratory Advantage Touch Button operation means simplicity Rapid Results in about 30 minutes for DNA purification Flexible Design means single samples can be run with no waste Batch Purify up to 16 samples at one time Diverse Purification kits and protocols available Economic Pricing of reagents and instrument MagCore HF 16 Introduction 1 Indicator LCD 2 LCM Display Screen 3 UV Key 4 Aluminum Hinge 5 Keypress Panel 6 Protective Acryl Board 7 Door Pull 8 Power Switch 9 UV Lamp 10 Door Open Detector 11 Door Pry 12 MagCore LCD Window 13 Syringe Unit 14 Operation Area Instrument Supplied Accessories Catridge Rack X 2 T Rack X 2 Empty Cartridge X 20 Tip Holder X 50 Tip X 50 Sample Elution Tube X 5
5. inal elution steps LCM Display Screen which with sapphire background shows clear information that the MagCore HF16 is currently performing Special function of buttons described as UV button is designed to turn on UV Lamp while your MagCore HF16 needs to be sterilized after runing contagious samples Options with 30 60 mins Shift button is designed for calibrating position accuracy at MagCore HF 16 System DO NOT excute this program without trained engineer aside Stop button is designed for going back to Stand By menu at program completed or for EMERGENCY STOP use Operation Procedures Turn on power switch LCM display screen shows located at the right hand MAGCORE HETE j Stand By as left picture side of MagCore HF16 A Piensa beep sound can be heard PRESS SSUARUS TO RUN and the machine is on Stand By condition Note At this step press UV button to perform UV decontamination After press Start button Input Catridge Code 201 machine runs program of to run Viral Nucleic Acid calibration initialize to DNA RNA protocol move all axis to original Catridge Code is shown on factory positions your Reagent Cartridge and the cover of user manual Confirm your input code Viral Nucleic Acid 400 200 INPUT CARTRIDGE CODE again and press Enter to protocols instaled 3 DIGITALS 201 next page for sample SENTER gt NEXT PAGE l volume selection Press 1 for 400ul sample Pres
6. rgency press STOP button at anytime LCM display screen shows warning sign Red Indicate LCD lights up and a beep sound can be heard After machnical dysfuntion eliminated select Start continue program Stop abort program and back to Stand By menu www rbcbioscience com Kit Contents 36 36 36 72 36 100 100 200 100 22C RBC Bioscience Corp RBC Bioscience is a leading R amp D based life science solutions provider BIOSCIENCE For more information on our extensive and innovative life science range please visit our web site
7. s 2 for 200ul sample Press Esc to re enter cartridge code Press 1 for 400ul sample WCAPTRIDCECODE D01aaeE Press Enter to next page At this step prepare racks to Press Esc to cancel whole ST ENCUIREARAGKSITGADEOSI operation area lt ESC gt CANCEL procedures and back to see Step 9 18 Stand By menu lt ENTER gt NEXT PAGE After racks loaded then press Press 2 for 200ul sample Enter to next page for Press Enter to next page elution volume selection Meare Tee rd Press Esc to cancel whole pli Ne ee oad procedures and back to Stand By menu Operation Procedures Insert MagCore Viral Nucleic Acid cartridge into Cartridge Rack following arrow point directions Load as many cartridges as sample runs Capacity for each Cartridge Rack is 8 up to 16 samples for two Cartridge Racks can be performed at one time on MagCore HF 16 Put Tips into Tip Holders Put a microcentrifuge tube onto hole 1 of T Rack as Elution Tube Note Sample Tube A Slide MagCore cartridge into Cartridge Rack to the end with slight downward angle B Press down the cartridge to interlock position under the over hang part of Cartidge Rack A click sound can be heard when it is locked correctly Instal the cartridges loaded Cartridge Rack into inner operation area of the MagCore HF 16 and lock firmly Set the Tip Tip Holder onto hole 2 of T Rack A Dissolve the Carrier RNA 16 2mg with 1ml of provided RNase
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