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Variable Temperature Thermal Sealer

1. 1306 884886 info 4ti co uk www 4ti co uk Page 4 Ov tude 7 MAINTENANCE 28 7 1 Regular Maintenance 28 8 ACCESSORIES AND CONSUMABLES 29 8 1 Plate Types 29 8 2 Thermal Sealing Plastic and Sealing Film Types 29 8 3 Plate Carriers and Accessories 30 9 SEALING PARAMETERS 31 10 SHIPPING INSTRUCTIONS 32 11 WARRANTY 33 APPENDIX 1 Atitude Consumables Heat Seals 34 4titude Consumables FrameStar PCR Plates 36 Atitude Consumables General PCR Plates 38 Atitude Consumables Storage Plates 39 Atitude Consumables Vision Plate Screening Plates 39 Atitude Consumables Assay Plates 40 Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 5 Ov tude 1 1 Introduction The variable temperature thermal Sealer 4s3 is a semi automatic instrument designed to seal sample plates made from various polymers The sealer can be used with most plate types and sealing films available Both temperature and time are variable and can be optimised to suit each sealing plate and film When the 4s3 is ready to start the operator needs to position the sample plate together with a foil or film on the plate carrier The sealing operation starts once the operator presses OPERATE Film and sample plate will then automatically be brought in contact with the heating platen As soon
2. sterile 24 plates 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 39 Ov tude Product Code Description Quantity 4ti 0225 Vision Plate 96 150 micron Collagen Type 1 coated sterile with lids Vision Plate 96 150 micron polyD Lysin coated sterile with lids 4ti 0241 Vision Plate 24 150 micron TC treated sterile with lids 24 plates 4ti 0241 700 Vision Plate 24 700 micron TC treated sterile with lids 24 plates 4ti 0242 Vision Plate 24 150 micron TC treated sterile 30 plates 4ti 0242 700 Vision Plate 24 700 micron TC treated sterile 30 plates 24 plates 4ti 0226 24 plates 3 Vision Plate 24 175 micron Collagen Type 1 coated 4ti 0245 sterile with lids AES Vision Plate 24 175 micron polyD Lysin coated sterile with lids 4ti 0246 24 plates 4titude Consumables Assay Plates Product Code Description Quantity Assay Plates 4ti 0263 96 well Assay Plate solid base plate black non sterile 100 plates 4ti 0264 384 well Assay Plate solid base plate black non sterile 100 plates 4ti 0274 384 well Assay Plate solid base plate white non sterile 100 plates 4ti 0254 384 well Assay Plate solid base plate clear non sterile 100 plates 4titude Ltd The North Barn Surrey Hills Business Park Damphur
3. 3730 roll for ABI 3730 sequencer 610m x 78mm Clear Seal 3730 for ABI 3730 sequencer sample roll 5m x 78mm 4ti 0581 Clear Seal 3730 for ABI 3730 sequencer perforated roll 1000 sheets Clear Seal 3730 roll for ABI 3730 sequencer for Velocity1 1 500m x 115mm Clear Seal 3730 roll for ABI 3730 sequencer for Velocity1 1 sample roll 5m x 115mm Ati 0585 DMSO resistant Peel Seal roll 500m x 78mm 1 roll 4ti 0585 REMP DMSO resistant Peel Seal roll 500m x 78mm 1 roll 4ti 0585S DMSO resistant Peel Seal sample roll 5m x 78mm 1 roll Ati 0586 DMSO resistant Peel Seal roll for Velocity11 500m x 1 roll 115mm 4ti 0586 REMP DMSO resistant Peel Seal roll 500m x 78mm KE oo Peel Seal sample roll for Velocity11 5m x Ati 0574S 1 roll Ati 0580 1 roll Ati 0580S 1 roll A4ti 0582 1 roll Ati 0582S 1 roll 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 35 ei tude Product Code Description Quantity 4ti 0592S Thermal Bond for sample roll for Velocity 11 5m x 115mm 1 roll 4ti 0595 Gas Permeable Seal sheets 125mm x 78mm 100 sheets 4ti 0596 Gas Permeable Seal roll 175m x 78mm 1 roll 4ti 0596S Gas Permeable Seal sample roll 175m x 78mm 1 roll 1 Compatible with Thermo fisher ALPS 300 ALPS 3000 KBiosystems Wasp KBioscience FlexiS
4. 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 22 Or tude Once the plate makes contact with the heating platen the display shows the symbol t and the countdown of the programmed sealing time starts When the countdown timer reaches zero the drawer automatically opesn The sealed plate can now be removed The unit emits an audible beep once the heating platen has reached the programmed temperature again and a new cycle can be started Note Foil backed film may remain hot for a number of seconds after sealing Care should be taken in handling these plates 5 2 Stand by Mode lf left idle for a prolonged time the sealer will automatically switch into stand by mode factory set at 60 minutes and the temperature of the heating plate will be reduced to 60 C When entering into stand by mode the display shows the decrease of the temperature in real time and the amber LED is switched off The temperature of the heating platen will be maintained at 60 C The display shows the actual temperature of the heating platen and not the set sealing temperature By pressing any key the unit will automatically begin to warm up the heating plate to the pre selected sealing temperature Refer to section 4 2 to change the time after which the unit switches into stand by mode or to switch off the stand by function Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lan
5. UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 31 Ovitude 10 SHIPPING INSTRUCTIONS When shipping the unit the original packaging must be used to prevent damage to the instrument The manufacturer accepts no responsibility for damage incurred unless the unit is correctly packed as described below and transported using the original packaging 1 Before shipping remove the plate carrier s from the unit Note If left inside the unit the plate carriers may cause severe damage to the sealer Replace protective elastic bands Push CLOSE to close the drawer Manually push the drawer fully closed 4 Switch off the unit Note if the unit is shipped without the protective elastic bands the front panel can be damaged during shipping 5 Place the sealer between the two foam inserts inside the original shipping box 6 Place the accessories in the smaller cardboard box and place on top of the foam inserts 7 Close the box using a suitable adhesive tape Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 32 Ori tude 11 WARRANTY The manufacturer warrants that the product is free from defects in materials and workmanship for a period of 12 months from the date of purchase The manufacturer warrants the services it performs and the s
6. as the sample plate is in contact with the heating platen a countdown timer starts and after the chosen sealing time the motor automatically moves the sample plate away from the heating platen and the operator can remove the sealed sample plate for further processing 1 2 Chapter Index To use the 4s3 correctly and to obtain the best results please read this manual carefully Chapter 1 Introduction Warnings and Technical Specifications Chapter 2 General Description Chapter 3 Installation Chapter 4 Set Up Chapter 5 Basic functions Chapter6 Troubleshooting Chapter 7 Maintenance Chapter 8 Accessories amp Consumables Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 6 Chei tude 1 3 Safety Before using the sealer please read the following safety notes This product must only be used in accordance with proper safety standards and procedures together with the instructions included in this manual The sealer has been designed and manufactured to conform to international safety specifications It s essential that the users know the potential hazards associated with the equipment All operators should know and observe the safety precautions and warnings given in this section before starting to use the instrument If the unit is used in a way not specified by the manufacturer the
7. down time is the time after which the heater is switched off automatically when the unit is left idle e The power down time can be decreased or increased with the O0 keys e Power down can be set between 1 hour and 24 hours with steps of 1 hour e Press the key again to store the new power down time and the display will flash with S and the current audible warning setting e The audible warning function can be enabled on or disabled no with the O keys e Press the key again to store the audible warning function and the display will flash with r this MUST BE set to on otherwise the drawer will not function correctly Press the key again to save and exit e The unit returns into run mode see Chapter 5 Basic Functions page 20 and the actual temperature of the heating platen is shown in the display Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 19 Oheitude 5 BASIC FUNCTIONS 5 1 Run Mode Switch on the unit The unit will automatically begin to warm up the heating platen to the last set temperature The display shows the temperature increase in real time with the amber LED flashing When the heating platen reaches the set temperature an audible beep is emitted and the amber LED remains permanently illuminated The system is ready f
8. protection provided by the equipment may be ineffective The following caution and safety signs are used in this manual WARNING Refer to manual The machine is designed for indoor laboratory use only at an altitude of less than 2200m above sea level within a temperature range of 15 C to 35 C and a relative humidity range of 5 to 85 non condensing If the instrument is stored outside these ranges it should be left to stand until it equilibrates to within the above limits Ensure that the voltage selection switch is set to the correct voltage and that the correct fuse for the required voltage setting is fitted Do not work outside the rated power supply range Use the cleaning method recommended by the manufacturer Ensure the unit is only connected to an earthed supply There are no user accessible or serviceable parts inside the unit Do not remove the unit s cabinet Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 7 Ov tude WARNING Hot surface The hot plate can reach temperature up to 200 C Care must be taken not to touch it or serious burns may occur The equipment will maintain a high temperature for a considerable time after switching off and it is essential to wait before starting cleaning operations Sealed sample plates may remain hot for some seconds af
9. Qs Variable Temperature Thermal Sealer Version C Software Version 1 07 03 Oct 13 Ov tude 4s3 VARIABLE TEMPERATURE THERMAL SEALER USER MANUAL All rights reserved No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language or computer language in any form or by any means electronic mechanical magnetic optical chemical or other without the prior written permission of the manufacturer The manufacturer reserves the right to revise this publication and to make changes from time to time in the contents hereof without obligation to notify any person of such revision or changes Unless otherwise specified references to names or parts are purely casual and are used to illustrate the product described The document is for information only The manufacturer accepts no liability for errors contained herein or for incidental or consequential damages with the performance or use of this material Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 1 Ov tude System Components The following components are part of the 4s3 System product code 4ti 0655 e 4ti 0655 4s3 Heat Sealer e Power Cable e Manual amp Quick User Guide e 4ti 0615 Plate Carrier e 4ti 0602 Weighted Platen Replaceme
10. amp 21 for plate carrier usage instructions Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 30 Oi tude 9 SEALING PARAMETERS The following sealing temperatures are for guidance only Sealing efficiency varies depending on the plate type used Add 1 second to the sealing time when a weighted platen is used 4ti 0602 Temperature C Dwell Time secs 96 well 384 well Vision A PCR Plates PCR Plates Plates 175 185 2 3 185 200 3 4ti 0581 Clear Seal 3730 165 175 3 165 175 2 175 185 2 3 Part Description number 4ti 0541 Clear Seal 175 185 3 170 175 2 3 160 175 2 160 175 2 185 200 3 165 180 2 160 175 2 3 185 200 3 185 200 3 170 180 2 3 160 170 2 165 175 3 165 175 3 175 185 3 Table 2 Sealing Temperatures and Times for different types of plates These values are for two component PCR plates For one piece polypropylene plates increase dwell time by one second These values can be used for all polystyrene plates All sealing parameters were estimated using software version 1 02 These values are for guidance only Itis advisable to conduct trials depending on the plate type being sealed 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT
11. display will flash with t and the current sealing time flashing e The sealing time can be decreased or increased with the O keys e Time can be set between from 0 1 second in 0 1 second increments and from 1 10 seconds in 0 5 second increments e Press the S key again to store the new sealing time value and this will also exit from the programme menu e The unit returns into run mode see Chapter 5 Basic Functions page 20 and the actual temperature of the heating platen is shown in the display Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 18 Oei tude 4 2 Stand by Power Down and Audible Warning Control To programme stand by and power down time and control the audible warning e Press the S key for 10 seconds then release e The display shows the symbol L flashing and the last set stand by time in minutes The stand by time is the time after which the heater temperature is automatically reduced to 60 C when the unit is left idle e The stand by time can be decreased or increased with the O 0 keys e Time can be set between 1 minute and 99 minutes with steps of 1minute or as no standby e Press the key again to store the new stand by time and then the display will flash with P and the current power down time in hours The power
12. e Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 23 Ov tude 5 3 Power down Mode If the unit remains idle in stand by mode for a prolonged time the system will go into power down mode and the heating platen will be switched off factory set at 10 hours The amber LED is switched off The display shows the temperature decrease until it reaches 45 C after this LO will be displayed By pressing any key the unit will automatically begin to warm up the heating platen to the pre selected sealing temperature Refer to section 4 2 to change the time after which the unit switches into power down mode 5 4 Closing Drawer When the unit is not in use to prevent damage or dust contamination the drawer can be closed by pressing CLOSE Note Do not close the drawer by hand 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 24 On itude 6 TROUBLESHOOTING 6 1 Introduction Possible error messages generated by the 4s3 are summarised in this chapter If the solutions given below do not solve the problem please contact Atitude or their authorised local distributor 6 2 Error Messages The display may show one of the following error codes An intermittent audible warning sound will be emitted at the
13. eal and Cube Compatible with Agilent Velocity 11 PlateLoc Compatible with REMP Portrait Heat Sealer PHS Compatible with REMP Landscape Stacking Heat Sealers LHS SHS Atitude Consumables FrameStar PCR Plates Product Code Description Quantity FrameStar 384well PCR Plates 4ti 0382 4ti 0381 plus 50 x qPCR adhesive seals 4ti 0560 50 plates 50 seals 4ti 0383 4ti O380C plus 50 x qPCR adhesive seals 4ti 0560 50 plates 50 seals Ati 0386 FrameStar 384 black frame black wells 50 plates Product Code Description Quantity FrameStar 96well PCR Plates Ati 0710 FrameStar 96 non skirted purple frame 50 plates 4ti 0710 B FrameStar 96 non skirted blue frame 50 plates 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 36 ei tude Product Code Description Quantity 4ti 0731 oo semi skirted with upstand black frame white 50 plates Ati 0770 C FrameStar semi skirted clear frame A12 cut 50 plates Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 37 ei tude Product Code Description Quantity 4ti 0953 4ti 0950 C plus 50 x qPCR adhesive seals 4ti 0560 50 plates 50 seals 4ti 0960 FrameS
14. follows Do not attempt to clean the unit until it has cooled down to near room temperature Set temperature to OFF refer to page 18 for instructions Allow unit to cool until heating platen is close to ambient If drawer is not open press OPERATE to open Switch unit off at back Disconnect from mains supply To allow better access to heating platen lay the unit gently onto the side Figure 24 Gently place the 4s3 onto the side to give easier access to heating platen Clean heating platen using soapy water and a damp cloth Once clean upright the unit e Ensure nothing is obstructing the drawer reconnect the mains switch on at the back of the unit o The drawer will automatically close 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 27 Ov itude 7 MAINTENANCE 7 1 Regular Maintenance The unit does not require regular maintenance but it should be kept clean The external casing of the heat sealer can be cleaned with a cloth dipped in water or ethanol methanol formaldehyde can also be used The unit should not be immersed in solvents Do not use acetone or abrasive cleaners No parts are to be autoclaved except adapters In case of radioactive spillages it is recommended to use an appropriate cleaning agent For any other maintenance operation n
15. ne Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 13 Gei tude 2 1 1 Control Panel Set up Run Mode Key OPERAT CLOSE Operate amp Directional COIR up Key Close Drawer amp Directional down Key Figure 10 Control Panel Three digit LED display Amber LED Status Indicator 2 1 2 Rear Power Interface Power Setting 115 230 Vac and Fuse Holder I O Power Switch Figure 11 Rear Interface Mains Power Plug 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 14 Ov tude 3 INSTALLATION 3 1 Unpacking and Start Up After unpacking retain all packing material and fixtures as the unit must always be transported in its original packaging to avoid damage The manufacturer accepts no responsibility for damage incurred unless the unit is correctly packed and transported in this way Remove the 4s3 unit from its packaging Open the sealing drawer and remove the protective elastic bands Once powered up the drawer will close automatically The unit has to be placed on a level surface away from direct sunlight ensuring access to the power switch at the back of the unit Ensure that the vents on the casing are not obstructed The mains voltage selector switch should be checked to ensu
16. nt accessories and optional extras are to be ordered separately e 4ti 0625 Plate carrier for 96well PCR Plates optional extra e 4ti 0612 Sealing Frame supplied FOC with 4ti 0625 e 4ti 0616 Roche 1536 Plate Adapter optional extra Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 2 Oei tude 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 3 Ov tude PAGE INDEX Page 1 INTRODUCTION 6 1 1 Introduction 6 1 2 Chapter Index 6 1 3 Safety 7 1 4 Applicable Rules 8 1 5 Technical Specifications 10 2 GENERAL SPECIFICATION 12 2 1 Sealer Parts 12 2 1 1 Control Panel 14 2 1 2 Rear Power Interface 14 3 INSTALLATION 15 3 1 Unpacking and start up 15 3 2 Voltage Check 16 4 SET UP 18 4 1 Set Up Operating Mode 18 4 2 Stand By Power Down and Audible Warning Control 19 5 BASIC FUNCTIONS 20 5 1 Run Mode 20 5 2 Stand by Mode 23 5 3 Power down Mode 24 5 4 Closing Drawer 24 6 TROUBLESHOOTING 25 6 1 Introduction 25 6 2 Error Messages 25 6 3 Cleaning the Heating Platen 27 Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0
17. or sealing Push OPERATE to open drawer and then the sample plate can be loaded onto the appropriate plate carrier Deep Well Plates All fully skirted SBS standard plates except 96well PCR plates can be loaded onto the standard plate carrier 4ti O615 Figure 16 Figure 16 Use the standard plate carrier 4ti 0615 for deep well plates Shallow Well Plates However for easier handling of shallow well plates we recommend to place the standard plate carrier 4ti 0615 inside the adapter for 96well PCR plates optional extra code 4ti 0625 Figure 17 Figure 17 The standard plate carrier 4ti 0615 can be placed inside the adapter for 96well PCR plates 4ti 0625 This is recommended for skirted shallow well plates such as 384well PCR plates 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 20 Gei tude 96well PCR Plates All 96well PCR plates should be inserted into the 96well PCR plate adapter product code 4ti 0625 Figures 18 amp 19 Note When using the 96well PCR plate adapter the standard plate carrier should be removed from the drawer ka O CICI S OOCOL OCOLO Figure 18 Plate adapter for 96well PCR Figure 19 Plate adapter for 96well plates optional code 4ti 0625 PCR plates shown with non skirted PCR plate Roche 1536well Plates For
18. ot indicated in this manual please contact 4titude Any cleaning procedure must be followed with the unit switched off the power cable disconnected and the heated parts at ambient temperature 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 28 Chei tude 8 ACCESSORIES AND CONSUMABLES The 4s3 is designed to be used with the following consumables 8 1 Plate Types Any of the following made from polypropylene polyethylene or polystyrene SBS format shallow well plates Deep well plates Tube blocks various PCR plates 8 2 Thermal Sealing Plastic or Sealing Film types Foil polypropylene laminate Clear polyester polypropylene laminate Clear polymer Thin clear polymer e Foil laminate Foil 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 29 Ov tude 8 3 Plate Carriers and Accessories we 8 384 well plates Kua 0625 Adapter for 96 well PCR plates 96 well PCR a plates Ai hatin Adapter for Roche 1536 Plates Roche 1536 a plates 4ti O602 Weighted Platen to hold down sealing sheets 4ti 0612 Sealing frame to hold down sealing sheets No for use with 4ti 0625 Refer to pages 20
19. pare and replacement parts provided for a period of 90 days from the date of the performance of such services and the date of shipment of the spare or replacement parts respectively The manufacturer warrants that its software enclosed with the product for a period of 90 days from the date its software is received by the customer shall perform substantially in accordance with the standard set forth in the user documentation related to such software Used refurbished products that are sold As Is are not covered by warranty Additional coverage plans may be purchased If the manufacturer receives note of defects or non conformance during the warranty period the manufacturer will at its option repair or replace the affected product The manufacturer will provide at no additional charge parts and labour for factory repairs The consumables and shipping costs are not covered under warranty Loss damage or defects resulting from transportation from external causes not imputable to the manufacturer from inadequate maintenance from improper use of the instrument e g operations prohibited or not detailed in the user manual are not covered under warranty The manufacturer does not warrant that the operations of the product will be uninterrupted or error free The purchaser must control that the installation has been made in a correct way in compliance with safety standard and law dispositions Atitude Ltd The North Barn Sur
20. perature Range Step Default Sealing time Range Step 0 1 second Step 1 10 seconds Default Stand by cycle Range Step Default Stand by temperature Power down time Range Step Default Power down temperature Audible Warning Options Default Ov tude OFF or from 120 C to 200 C 1C OFF O to 10 seconds 0 1 second 0 5 second 3 seconds no stand by or from 1 to 99 mins 1 minute 60 minutes 60 C not programmable 1 to 24 hours 1 hour 10 hours Ambient ON OFF ON 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 11 Gei tude 2 GENERAL SPECIFICATION 2 1 Sealer Parts 3 4 i 350VA 50 60Hz NOV fuses T2A be i N Figure 2 Rear Power Interface Figure 3 Heating Platen Figure 4 Plate Carrier 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 12 Figure 5 96well PCR Plate Adapter 4ti 0625 Figure 6 Standard Plate Adapter 4ti 0615 optional Figure 7 Roche 1536 Plate Adapter 4ti 0616 Figure 8 Weighted Platen 4ti 0602 a a q Figure 9 Sealing Frame 4ti 0612 optional 4titude Ltd The North Barn Surrey Hills Business Park Damphurst La
21. re that the voltage has been set at the correct value Connect the unit to the mains power supply and switch on the switch I O of the rear power interface If the heater within the unit is receiving power the amber LED on the control panel will be illuminated After switching on wait until the amber LED light stops flashing the set temperature is reached and the unit is ready to seal Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 15 Oeitude 3 2 Voltage Check Be sure that the equipment is not connected to the mains power At the rear of the 4s3 is the connection panel with e Power setting 115 230 Vac and Fuse Holder e 1 O Power Switch e Mains Power Plug The set voltage is displayed in white on red background on the power setting and fuse holder see Figure 11 page 14 Figure 12 Opening fuse holder Figure 13 Levering out fuse holder compartment Figure 14 Correct fuse location Figure 15 Incorrect fuse location 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 16 Ov tude Changing the Voltage Setting Open fuse holder compartment with a screwdriver Figure 12 2 With the screwdriver lever out the fu
22. rey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 33 Ohitude APPENDIX I 4titude Consumables Heat Seals Product Code Description Quantity Heat Seals 4ti 0520 Peel Seal roll 610m x 78mm 1 roll j 4ti 0531 Pierce Seal sheets 125mm x 78mm 100 sheets 4ti 0532 Pierce Seal roll for Velocity11 500m x 115mm 1 roll H 4ti 0540 Clear Seal roll 500m x 78mm 1 roll 4ti 0540 REMP Clear Seal roll 500m x 78mm 1 roll 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 34 Oei tude Product Code Description Quantity 4ti 0540S Clear Seal sample roll 5m x 78mm 1 roll 4ti 0541 Clear Seal sheets 125mm x 78mm 100 sheets 4ti 0542 Clear Seal roll for Velocity11 350m x 115mm 1 roll 4ti 0542 REMP Clear Seal roll 500m x 115mm 1 roll 4ti 0542S Clear Seal sample roll for Velocity11 5m x 115mm 1 roll 4ti 0547 PS Foil Seal sheets 125mm x 78mm 100 sheets 4ti 0573 Clear Weld Seal Mark II roll 610m x 78mm 4ti 0573S Clear Weld Seal Mark II sample roll 5m x 78mm 1 roll 1 roll Clear Weld Seal Mark II sample roll for Velocity11 5m x 115mm 4ti 0575 Clear Weld Seal Mark II sheets 125mm x 78mm 100 sheets Clear Seal
23. same time Message defined time position The motor cannot move the lifting platform towards the heating plate within a defined time The motor cannot move the lifting platform to the low position within a defined time Motor over current Temperature sensor opened or disconnected Platform in low position sensor active during elevation movement Obstruction of the drawer whilst opening Obstruction of the drawer whilst closing Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 25 Ov tude The errors will remain displayed until the problem is solved To clear the error press CLOSE If any of the above errors persists switch OFF the unit and then switch ON again If within 10 seconds the problem is not solved the heated platen is automatically turned off to avoid sample damage If the problem is solved within 10 seconds the unit will automatically return to Run Mode Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 26 Ov tude 6 1 Cleaning the Heating Platen If the sealing film is placed upside down on the plate the sealing film will become stuck to the heating platen To clean it proceed as
24. se holder Figure 13 Replace the two fuses according to the table below Power Voltage EN 60127 115V 3 15A T 230V Table 1 Fuse ratings for 4s3 Insert the fuse holder keeping the set voltage label at the top Close the fuse compartment by pressing gently 6 If the fuse holder is inserted correctly you can read the voltage selected Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 17 Ovitude 4 SET UP 4 1 Set Up Operating Mode Turn on the 4s3 switch located at back of the unit The display will show the software version for a couple of seconds and then show the temperature to which the 4s3 is currently set The unit is delivered with the heater platen turned off If this is the first time of switching the unit on the keypad will display oFF Otherwise when switched on the unit will start to heat up to the last set temperature and the LED light will flash whilst it is heating up To programme time and temperature settings e Briefly press and release the S set key e The display shows the last set sealing temperature flashing e The temperature can be decreased or increased using the O keys e Temperature can be set between 120 C and 200 C with steps of 1 C or to OFF e Press the key again to store the new set temperature and then the
25. st Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 40
26. tar 96 skirted purple frame 50 plates 4ti 0960 B FrameStar 96 skirted blue frame 50 plates 4titude Consumables General PCR Plates Product Code Description Quantity General PCR Consumables 4ti 0740 96well skirted PCR plate 50 plates Ati 0741 96well skirted PCR plate white 50 plates 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 38 Chei tude 4titude Consumables Storage Plates Product Code Description Quantity Storage Plates 4ti 0116 350ul 96 round well U bottom plate polypropylene clear 100 plates 4ti 0117 330ul 96 round well V bottom plate polypropylene clear 100 plates 4ti 0120 1 2ml 96well low profile deep well plate 50 plates 4ti 0135 96well cap mat for 1 2ml deep well plate 100 mats Ati 0126 1 2ml 96 square well U bottom deep well plate 100 plates polypropylene clear Ati 0136 2 2ml 96 square well U bottom deep well plate 50 plates polypropylene clear 4titude Consumables Vision Plate Screening Plates Product Code Description Quantity Vision Plate Screening Plates see page 2 for Bar Code pricing 4ti 0204 700 Vision Plate 384 500 micron 30 plates A Vision Platen 384 150 micron Collagen Type 1 coated 4ti 0205 sterile with lids an piles T ae Ati 0206 nae 384 150 micron polyD Lysin coated
27. ter sealing and should be handled with care AN AN S WARNING Risk of electrical shock high voltage This equipment should only be dismantled by properly trained personnel removing the top case exposes potentially lethal mains voltages 1 4 Applicable Rules 2006 95 CE Low Voltage Directive 2004 108 CE Electromagnetic Compatibility Directive 2002 96 CE amended 2003 108 CE WEEE Directive Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 8 Oei tude 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 9 1 5 Technical Specifications MECHANICAL Dimensions Height Depth Width Weight ELECTRICAL Voltage Frequency Power ENVIRONMENTAL Temperature range Storage Relative humidity CONNECTION Serial port SOFTWARE Version Ov tude 310 mm 2 5mm 181 mm 10 kg 230 115 100 10 Vac 47 63 Hz 300 W Operative 18 C to 35 C 20 C to 40 C 20 to 80 not condensing RS 232 1 02 Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 10 SET UP PARAMETERS Sealing tem
28. use with a Roche 1536 plate insert the Roche 1536well plate adapter 4ti 0616 inside the adapter for 96well PCR plates optional extra code 4ti 0625 Figures 20 amp 21 S Figure 20 The Roche 1536 adapter 4ti 0616 should be placed inside the adapter for 96well PCR plates 4ti 0625 This adapter is required for Roche 1536well plates 4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 21 Ov tude Place the sealing film over the plate ensuring that the sealing surface is face down Figure 20 When using heat sealing films that have a tendency to curl for example 4titude Peel Seal product code 4ti 0521 DMSO Resistant Seal 4ti 0587 and Clear Weld Seal Mark II 4ti 0575 use the sealing frame which is supplied with the 4ti 0625 adapter For deep well plates place the weighted platen on top of the sealing film Figure 21 For most other plates we recommend the use of the sealing frame Figure 22 Figure Position Figure 22 Position Figure 23 Position sealing film foil on weighted platen over sealing frame over plate sealing film foil sealing film foil Press OPERATE The drawer will automatically close and the sealing process will begin automatically Atitude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5

Variable Temperature Thermal Sealer

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