ISO-OXY-2 Instruction Manual - World Precision Instruments
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1. 7 If the sensor reading was near zero when dry and extremely high in solution the sensor itself is probably working properly Install a new membrane sleeve See Replacing the Membrane Sleeve on page 10 TIP Do not reuse an old membrane because the membrane itself is stretched once it is used After it is removed it maintains the surface deformity and will not provide accurate test results if reused NOTE If you have a problem issue with your sensor that falls outside the definitions of this troubleshooting section first perform the Calibration Procedure exactly as describe on page 5 of this manual and contact the WPI Technical Support team at 941 371 1003 or technicalsupport wpiinc com SPECIFICATIONS The ISO OXY 2 OXELP sensor conforms to the following specifications Outside DIAM Otel iarria RA A RE 2mm Response TIME secs iretee aeoea AEA T TEER EAE TEE ER TE lt 10 sec Lowest Detection Limit Range srcsgsssnsiciapnoenien inienn 0 1 Upper Detection Limit Range 100 Nominal Sensitivity New SCNSON s essessseesseecseesseecsneesseeeeeesneesseesseenneesneesneennees 0 3 0 6nA BA AA AA ERRAR lt 1 min POISE Volta Css stscs ceescssscesces aesctcpevacesstccacsdvcecosesasdeclcqscadactaccndoashscsasdeusnucesdiececseacctyenadeasesniateowd 700mV Typical Quiescent Baseline Current 25 C eesessseeseeseeseesseeseeseeeseeseesneeseesseeseeneeseenees 15 40nA Acceptable Baseline RANG ecsessessesessesseseesnsecnees2. WPI tests ISO OXY 2 and OXELP sensors using a 0 1M PBS test media at room temperature A sensor is tested at three known oxygen levels 0 21 and 100 Known concentrations are obtained as follows World Precision Instruments 5 0 O2 PBS standard test media is purged with 100 N2 21 O2 The atmospheric concentration of O2 is obtained using unsaturated PBS solution exposed to air 100 O2 PBS is purged with 100 O2 gas To calibrate 1 Fill three 20mL vials with approximately 15 18mL of 0 1M PBS buffer solution Label the first 0 O2 the second as 21 Oa and the third as 100 O2 To obtain 0 O2 concentration insert an 18 gauge needle into the 0 O2 vial and vigorously bubble it with N2 gas at a constant rate for 10 minutes to displace any atmospheric oxygen Drop a small stir bar into the vial with 0 O2 solution and place it on a magnetic stirring plate Turn on the stirrer so that the bar is stirring at a moderate rate NOTE When using the stirrer its rate should not be modified once It is set NOTE Do NOT let the sensor tip come into contact with a stir bar It could damage the membrane Immerse the polarized sensor in the solution and secure it in an electrode holder such as WPI s Pro Guide WPI 47510 47520 47530 47540 ora micromanipulator The sensor tip should be immersed about 2 3mm into the solution Allow the sensor to remain in the solution at least five minutes as you view the data
3. Looking at the data record the current value nA of the sensor at 0 O2 in a table like the one below The current value recorded should be taken from the most stable flattest part of the recorded data NOTE For greater accuracy readings may be taken at 30 seconds 3 minutes and 15 minutes for each oxygen concentration as long as the N and O are continuously bubbled into the solutions Results can then be averaged foo Sensor Current 100 241nA Drop a small stir bar into the vial with 21 O2 solution and place it on the magnetic stirring plate Immerse the sensor in the second solution for at least five minutes The oxygen measurements should return to the baseline value Record the current value nA of the sensor at 21 Oz in the table For 100 O2 concentration insert an 18 gauge needle into the 100 vial and vigorously bubble it with 100 O2 gas at a constant rate for 10 minutes to saturate the solution Drop a small stir bar into the vial with 100 O2 solution and place it on the World Precision Instruments ISO OXY 2 OXELP magnetic stirring plate Immerse the sensor in this third solution for at least five minutes In the table record the current value nA of the sensor at 100 Oz 8 Return the sensor to the 21 O2 solution for at least five minutes The oxygen measurements should return to the baseline value The data should look similar to the graph in Fig 4 AA a ay a a or 350 300 f 250
4. Q Internal O2 sensing pair of working and counter reference electrodes Probe handle O Locking cap attaches the sleeve to the probe handle When the sensor is fully assembled with locking cap and sleeve in place the internal electrode should press gently against the polymeric membrane which will be slightly stretched This ensures that the electrolyte diffusion is as thin as possible minimizing sensor response time NOTE Once a membrane is stretched it is permanently deformed and cannot be reused if the sleeve is removed from the electrode Additional membrane sleeves are available in packages of 4 with electrolyte filling solution WPI 5378 The start up kit WPI 5377 also includes replacement membrane sleeves along with all the accessories to fill them properly with electrolyte solution 4 World Precision Instruments ISO OXY 2 OXELP OPERATING INSTRUCTIONS TIP Refer to your free radical analyzer manual for proper grounding and shielding techniques In the TBR4100 TBR1025 manuals see Grounding and Noise Concerns in the Operating Instructions section Polarizing the Sensor 1 Place the sensor in 0 1M PBS solution 2 Plug it into the monitoring device 3 Turn on the monitoring device 4 If necessary set the poise voltage On the TBR4100 1025 set the Probe Select switch to 02 on the Apollo1000 set the poise voltage to 700mV Set the range to 100nA 6 The sensor should be allowed at least one ho
5. f C 200 Oxygen nA Temp 150 F 100 L O j 50 kd AY mee t enna ANA a nr nn Time Seconds Fig 4 Calibration record showing established baseline at point 1 096 O2 reading at point 2 baseline re established at point 3 and 100 O2 reading at point 4 9 Construct a standard calibration curve using the recorded data Using a third party spreadsheet with graphing capability like Microsoft Excel it is possible to generate a linear regression analysis that will display the equation and the R coeffiecient To do this in Excel enter the data and generate a scatter plot graph Then select the line and right click Choose Add Trendline The Add Trendline dialog box appears Select the Linear radio button the Display equation on chart checkbox and the Display R value on chart checkbox Fig 5 World Precision Instruments 7 Sensor Current nA 300 250 y 2 4118x 0 6101 R 0 9998 200 150 Sensor Current nA Linear Sensor Current nA 100 50 100 120 50 Fig 5 Calibration output The slope of this curve indicates the sensitivity of the sensor Once the sensitivity of the probe has been ascertained the sensor is ready to use experimentally 8 World Precision Instruments ISO OXY 2 OXELP SENSOR CARE AND MAINTENANCE Durability and Handling The sensor is relatively durable except for the memb
6. it from the monitoring device and stored it with the tip immersed in distilled water This practice reduces the possibility of a gradual reduction of electrode surface activity as discussed above under long term polarization LONG TERM For long term storage of more than one week remove the membrane sleeve clean the sensor tip with deionized water and dry carefully Protect the tip and store the sensor with the membrane removed in a dry cool environment Cleaning the Membrane The membrane sleeve itself requires very little maintenance The primary concern is to avoid damage to the membrane and to keep it as clean as possible After each use the membrane should be cleaned by suspending the tip in distilled water for 20 30 minutes to dissolve salts and remove particles which may have accumulated on it If the probe was used in a protein rich solution the tip should first be soaked in a protease solution World Precision Instruments 9 for several minutes to remove protein build up and then in distilled water Enzymatic detergent for example Enzol WP1 7363 can also be used Accumulated organic matter can be removed by briefly immersing the tip in a 0 1M HCI or 0 1M NaOH at times both may be necessary for 10 seconds A good indication of a dirty membrane sleeve is a sluggish response or an unusually low sensitivity If these problems are not rectified by cleaning then the membrane sleeve should be replaced Sterilizing the Membran
7. the 1p A range the current rises rapidly off scale Then the current value will beging to fall Using the MicroFil nonmetallic syringe needle WPI MF28G67 5 and 1mL plastic syringe included in the start up kit inject approximately 100uL of electrolyte directly into the new sleeve starting about half way down the sleeve and drawing the MicroFil out of the sleeve as it fills The filling process should be performed slowly enough so as not to create turbulence 10 World Precision Instruments 10 11 ISO OXY 2 OXELP which could introduce air bubbles into the electrolyte The MicroFil MF28G67 included in the startup kit is less than the length of the sleeve so that it will not puncture the delicate membrane at the tip of the sleeve during injection TIP If air bubbles form in the The internal electrode tip electrolyte gently flick or tap the should protrude slightly side of the sleeve to remove the GUNItokheimemalane bubbles Slowly and smoothly insert the Fig 6 Membrane placement The internal electrode into the sleeve and screw lectrode tip should protrude slightly out into the the locking cap into the handle membrane The electrode should be observed to press gently against the membrane Fig 6 The current displayed on the meter at this time will be high or offscale Suspend the tip of the newly assembled probe in 0 1M PBS buffer solution After 10 15 minutes the current should no longer be offscale and
8. will gradually decrease with time It may take up to one hour for the sensor current to reach a low stable value at which time it will be ready for use TIP The integrity of the new membrane can be determined by immersing the probe tip into a strong saline solution 1M If the current increases dramatically or is offscale then the membrane integrity is not good and a new membrane will have to be fitted Additional membranes packages of 4 with filling solution are available from WPI 5378 World Precision Instruments 11 ACCESSORIES Part Number Description 47510 ProGuide Position Holder with Base 47520 ProGuide Position Holder 47530 ProGuide Plus Position Holder 47540 ProGuide Plus Position Holder with Base 5399 TAdapter Kit pkg of 3 5377 ISO OXY 2 OXELP Startup Kit 5378 Replacement Sleeves pkg of 4 and filling solution 7326 ISO2 Filling Solution electrolyte 7363 4 Enzol Enzymatic detergent 1 gal MF28G67 5 MicroFil electrolyte filling needle pkg of 5 The start up kit WPI 5377 contains everything needed to begin working with this sensor and the kit is highly recommended for first time users Fig 7 The kit includes e Calibration bottle e Two additional membrane sleeves Sold in packages of four with filling solution WPI 5378 e Electrolyte filling solution WPI 7326 MicroFil electrolyte filling needle WPI MF28G67 5 e ImL syringe WPI 3563 Fig 7 ISO OXY 2 OXELP St
9. NSOR WITH DEIONIZED WATER AND STORE THE SENSOR DRY In some cases the sensor is shipped dry See Replacing the Membrane Sleeve on page 10 2 World Precision Instruments ISO OXY 2 OXELP Parts List After unpacking verify that there is no visible damage to the sensor Verify that all items are included 1 ISO OXY 2 or OXELP O sensor 1 cc Syringe MF28G67 MicroFil 7326 10mL vial of filling solution Instruction Manual Sensor Performance Evaluation Unpacking Upon receipt of this sensor make a thorough inspection of the contents and check for possible damage Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed damage should be reported at once to the carrier and an inspection requested Please read the section entitled Claims and Returns on page 15 of this manual Please contact WPI Customer Service if any parts are missing at 941 371 1003 or customerservice wpiinc com The sensor is shipped in a sealed rigid plastic hinged box with foam padding to avoid damage to your sensor during shipment The tip of the sensor is protected by inserting it into a small plastic vial containing deionized water DIW to keep the membrane from drying out NOTE In some cases the sensor is shipped dry To open the package carefully cut the seals on either side of the sensor box Inside the box on top of the sensor are the important documents to read
10. arr SO OXY 2 OXELP Macrosensor for O Measurement INSTRUCTION MANUAL Serial No 011215 World Precision Instruments Other WPI Favorites Free Radical Analyzer e Real time detection using electrochemical microsensors e Measure nitric oxide from lt 0 3 nM to 100 uM e Measure hydrogen peroxide lt 10 nM to 100 mM e Measure hydrogen sulfide e Measure glucose e Measure oxygen from 0 1 to 100 e Integrated system includes one temperature sensor your choice of two additional sensors and a start up kit Current measurement range from 300 fA to 10 HA four ranges permits wide dynamic range for detection Wide bandwidth allows recording of fast events e Isolated architecture allows Lab Trax interface to simultaneously measure free radical and independent analog data i e ECG BP etc data on any channel T E M Pp E RA A T u R E N MACRO SENSORS Order Number ISO COP 2 ISO NOP ISO HPO 2 ISO OXY 2 ISO H2S 2 Available Diameters 2mm 2mm 2mm 2mm 2mm Response Time lt 10 sec lt 5 sec lt 5 sec lt 10 sec lt 5 sec Detection Limit Range 10nM to 104M 1 NM to 40uM lt 100nM to 0 1 100 lt 5nM 100uM 100uM Sensitivity 0 5 pA nM lt 2 pA nM 8 pA uM 0 3 0 6nA 2 pA nM Drift lt 1pA min lt 1pA min 0 1pA min lt 1 min Temperature Dependent Yes Yes Yes Yes Yes Physiological Interference nitric oxide NaNO 10 or None None None better Replacem
11. artup kit 12 World Precision Instruments ISO OXY 2 OXELP TROUBLESHOOTING Issue Possible Cause Solution The poise voltage sensor setting Set the poise voltage to 700mV For the may be incorrectly set TBR choosing the O sensor setting selects 700mV automatically Set the range at 10nA The sensor may be nearing the Perform a standard calibration with at least end of its usable life three points If the sensor responds linearly within the desired concentration range it is still useable See Calibration Procedure on page 5 The calibration should show that the sensor responds in a linear fashion The membrane may have an air Replace the membrane sleeve In some bubble in the tip cases it is possible to remove the sleeve tap gently to remove the air bubble and refill it If the baseline hasn t stabilized Prepare fresh polarizing solution Use 0 1M after an hour the polarizing PBS only solution may be contaminated External electrical interferences Identify and isolate electrical interferences may be the problem The dilution factors may be See Calibration Procedure on page 5 incorrect Verify the procedure used Unsaturated solutions may be Be sure the bubble the test solutions with used 100 N and 100 O for at least 10 minutes before taking measurements If the test solutions are allowed to stand without bubbling gas into them the O concentration degrades Membrane is old o
12. before handling the sensor including the instructions for removal of the sensor tip from the protection vial Keep the sensor tip in this vial until the sensor is ready for use KEEP THE SENSOR STORAGE BOX and all the documentation in a safe place The test date and serial number of each sensor is printed on the bottom of its box Use of the sensor should begin within 30 days of receipt Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department Goods returned unauthorized by collect freight may be refused If a return shipment is necessary use the original container if possible If the original container is not available use a suitable substitute that is rigid and of adequate size For further details please read the section entitled Claims and Returns on page 15 of this manual World Precision Instruments 3 INSTRUMENT DESCRIPTION Structure of the Sensor The basic structure of the ISO OXY 2 and the OXELP sensors is shown below Fig 3 The figure shows an ISO OXY 2 connector Fig 3 ISO OXY 2 Sensor assembly Gas permeable polymeric membrane covering the end of the stainless sleeve to separate it from the external environment Q Disposable protective stainless steel sleeve WP1 5378 that houses the sensitive electrode pair The sleeve is flanged to properly connect with the locking cap It must contain fresh electrolyte WP1 7326
13. e The membrane sleeves can be sterilized chemically using an appropriate disinfectant for example Cidex WP1 7364 CAUTION Do not use alcohol on the sensors and do NOT expose them to organic solvents Replacing the Membrane Sleeve Even with the best of care and proper maintenance the membrane sleeve will eventually need to be replaced 1 Unscrew the locking cap from the handle 2 Hold the stainless steel sleeve and remove it and the locking cap from the internal electrode assembly being careful not to bend the internal electrode assembly when doing so 3 Rinse the internal electrode with distilled water particularly the tip and let it soak for at least 15 minutes Be careful not to let water get up into the handle 4 Gently dry the sensor with a soft tissue Kimwipes Be sure to dry thoroughly the flat surface at the tip of the electrode After drying the current should stabilize fairly quickly to a low value for example 0 200pA If this occurs it is a good indication that the electrode is functioning properly 5 If the electrode is not clean repeat steps 3 and 4 6 Remove the locking cap from the old used sleeve and gently slide it onto the new replacement sleeve Dispose of the used sleeve Additional membrane kits with sleeves and filling solution WP1 5378 may be purchased separately 7 Dip the internal electrode 1 2cm into the ISO2 Filling Solution WPI 7326 included in the start up kit WPI 5377 In
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15. ent Sleeves pkg of 4 95620 5436 600012 5378 600016 Filling Solution 95611 7325 100042 7326 100084 Start up Kit 95699 5435 600011 5377 600015 Higher detection limit available on request call for custom pricing ISO OXY 2 OXELP CONTENTS ABOUT THIS MANUAL nananana nananana nsan ann mmmrssuman 1 INTRODUCTION naa ENGR KANTA ANTE R ANG RANNIE 2 Notes and Wanig S ama AANI BA MINANA 2 INSTRUMENT DESCRIPTION 0 eseeeseeseesseessseesseesseessessnessutssseesseesatesstesseessueesutesneessessessessneesnesy 3 UNPACKING eos aNG NANANA ANAN NANAMIN NENA E 3 Structure of the SONS OM ann AANGAT 4 OPERATING INSTRUCTIONS nanasa mak NANkAKEAN KNA ANAKAN S E si 5 POIAMIZINE TE SENSON aaa au nana aamunaia aan BA taas ASERS 5 Calibration PrOCSCUNG ss AAAAAAAAAAA AAAAAA 5 SENSOR CARE AND MAINTENANGE namana nun GN mainan aa aamin 9 Durability and Handling BS SILO AA AA AA R Cleaning the Membrane mamam A ANNA ANA AA NANANA Sterilizing the Membrane Replacing the Membrane Sleeve NG IANG PAA nnan E A E EE E AAR ORO TROUBLESHOOTING mmm A ETN in Diy Sensor AA AA AA AA E SEER iraia SPECIFICATIONS aaa Na Naan AE EREE OE WARRANTY eaan aa E ATN RRR Claims and REtUINS u L L A NAAN NN BK nmammmmmmmnmmmnnnmmma RepalB E E E NATA AA AA AA AA Copyright 2015 by World Precision Instruments Inc All rights reserved No part of this publication may be reproduced or translated into any language in any for
16. m without prior written permission of World Precision Instruments Inc World Precision Instruments World Precision Instruments ISO OXY 2 OXELP ABOUT THIS MANUAL The following symbols are used in this guide This symbol indicates a CAUTION Cautions warn against actions that can cause damage to equipment Please read these carefully This symbol indicates a WARNING Warnings alert you to actions that can cause personal injury or pose a physical threat Please read these carefully NOTES and TIPS contain helpful information Fig 2 The OXELP sensor works with the ISO2 only World Precision Instruments 1 INTRODUCTION The ISO OXY 2 and the OXELP are 2 0mm oxygen sensors with replaceable stainless steel membrane sleeves filled with an electrolyte solution Replacement membrane sleeves pkg of four and filling solution can be purchased in a kit WPI 5378 Filling solution can also be purchase separately WPI 7326 A startup kit for this sensor is available and is recommended for the first purchase of this sensor type WPI 5377 With the exception of the connector the ISO OXY 2 and the OXELP sensors are identical The ISO OXY 2 is designed for use with WPI free radical analyzers and the OXELP is designed for use with the ISO2 dissolved oxygen meter Operation maintenance and specifications are identical for these two sensors so they will be discussed side by side in this manual These sensor incorporates WPI s
17. nt that any of its equipment is furnished by a manufacturer other than WPI the foregoing warranty shall be applicable only to the extent of the warranty furnished by such other manufacturer This warranty will not apply to appearance terms such as knobs handles dials or the like WPI makes no warranty of any kind express or implied or statutory including without limitation any warranties of merchantability and or fitness for a particular purpose WPI shall not be liable for any damages whether direct indirect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed loss or damage should be reported at once to the carrier and an inspection requested All claims for shortage or damage must be made within ten 10 days after receipt of shipment Claims for lost shipments must be made within thirty 30 days of receipt of invoice or other notification of shipment Please save damaged or pilfered cartons until claim is settled In some instances photographic documentation may be required Some items are time sensitive WPI assumes no extended warranty or any liability for use beyond the date specified on the con
18. proprietary combination electrode technology whereby the oxygen sensing element and separate reference electrode are encased within a single shielded sensor design A gas permeable polymer membrane is fitted over the end of the sleeve which allows oxygen to pass while blocking liquids ions and particulate matter Oxygen diffuses through the membrane The voltage applied to the sensor is held at 0 7V when the monitoring device is on and the sensor is properly connected The magnitude of the generated electrical current is determined by the rate of diffusion through the membrane The rate is proportional to the partial pressure of oxygen outside the membrane The current serves as a measure of the partial pressure of O Notes and Warnings OD CAUTION DO NOT EXPOSE SENSOR TO ORGANIC SOLVENTS Q CAUTION Carefully read the Probe Unpacking instructions found in the sealed sensor case before handling the sensor NOTE The sensor must be polarized for at least one hour in 0 1M PBS solution prior to use NOTE The sensor tip and membrane are extremely delicate Improper handling will lead to damage of the sensor NOTE For optimal use of each sensor and sensor membrane sleeve the sensor must be stored properly See Storing the Sensor on page 9 Pay special attention to the sticker on the box It says THE SENSOR SLEEVE IS FILLED WITH ELECTROLYTE IF THE SENSOR IS NOT TO BE USED WITHIN ONE WEEK REMOVE THE SLEEVE CLEAN THE SE
19. r worn or In either case Replace the membrane electrolyte solution evaporated _ sleeve See Replacing the Membrane Sleeve on page 10 Baseline current is below specified range vo ss Gro BY Cc 0 IsO not linear Sensitivity Calibration dataset is below range specified Dry Sensor Test To determine if the sensor itself is defective or has a short you may perform a dry sensor test on a sensor without a sleeve 1 Carefully remove the membrane sleeve See Replacing the Membrane Sleeve on page 10 World Precision Instruments 13 NOTE If the sensor has not been used for several months the electrolyte solution may have dried out If this is the case the membrane sleeve is more difficult to remove because it tends to stick to the sensor Remove it gently to avoid damaging the delicate sensor tip Remove any crystals and lightly wash the sensor with distilled water Allow sensor to air dry When the sensor is completely dry plug it into the free radical analyzer gi 5 YN Turn on the free radical analyzer You should see a near zero reading of about 200pA If the reading is higher the sensor is defective and needs to be replaced 6 If the sensor passed the test in step 5 insert the sensor into a vial with PBS solution The free radical analyzer should immediately display a high nanoamp reading If the analyzer is set in the 1pA range the reading will peak between 60 300NA and then begin to fall
20. rane sleeve Exercise caution when handling any sensor to avoid actions that could damage the sensor tip Pay particular attention to the sensor membrane because the membrane is extremely delicate and improper handling will lead to damage Refer to the Probe Unpacking Instructions that came with your sensor for handling instructions CAUTION Do NOT scratch the sensor membrane sleeve Do NOT wipe the sensor membrane with anything even Kimwipes If necessary squirt it with distilled water or compressed air CAUTION The sensor membrane is easily punctured if it comes into contact with sharp objects For example do NOT let the stir bar come into contact with the sensor membrane Storing the Sensor The reduction of oxygen and other trace impurities causes a decrease in the surface activity of the working electrode This phenomenon is referred to as poisoning and over time has the effect of gradually reducing the electrode s capability to generate a sufficient redox current With proper care and by following the instructions below a membrane sleeve should last more than one month Use the following guidelines to maximize the life of the electrode STANDBY If the oxygen sensor is being used on a daily basis leave the monitoring device ON continuously with the sensor plugged in and its tip suspended in distilled water to maintain polarization SHORT TERM If the sensor is not to be used for a period of more than 2 3 days disconnect
21. sessesssessecsesseesesseecueeneesuetseesnesneesterees 15 80nA Polarization THM iscsveciccsvczecstassscsvesdessneusestuetuedesisaizeocsessisiaclivestassarsteessuaracgabsebnatebys tines 1 hours 95 Recommended Polarization Solution c ce cceccccsesesesesescsescscscscscscsescseeesesesescsescsesssteceees 0 1M PBS Temperature Dependent Physiological Interference 14 World Precision Instruments ISO OXY 2 OXELP WARRANTY WPI World Precision Instruments Inc warrants to the original purchaser that this equipment includ ing its components and parts shall be free from defects in material and workmanship for a period of 30 days from the date of receipt WPI s obligation under this warranty shall be limited to repair or replacement at WPI s option of the equipment or defective components or parts upon receipt thereof f o b WPI Sarasota Florida U S A Return of a repaired instrument shall be f 0 b Sarasota The above warranty is contingent upon normal usage and does not cover products which have been modified without WPI s approval or which have been subjected to unusual physical or electrical stress or on which the original identification marks have been removed or altered The above warranty will not apply if adjustment repair or parts replacement is required because of accident neglect misuse failure of electric power air conditioning humidity control or causes other than normal and ordinary usage To the exte
22. tainer Do not return any goods to us without obtaining prior approval and instructions from our Returns Department Goods returned unauthorized by collect freight may be refused Goods accepted for restocking will be exchanged or credited to your WPI account Goods returned which were ordered by customers in error are subject to a 25 restocking charge Equipment which was built as a special order cannot be returned Repairs Contact our Customer Service Department for assistance in the repair of apparatus Do not return goods until instructions have been received Returned items must be securely packed to prevent fur ther damage in transit The Customer is responsible for paying shipping expenses including adequate insurance on all items returned for repairs Identification of the item s by model number name as well as complete description of the difficulties experienced should be written on the repair purchase order and on a tag attached to the item Electrodes batteries and other consumable parts are warranted for 30 days only from the date on which the customer receives these items World Precision Instruments 15 World Precision Instruments Inc USA International Trade Center 175 Sarasota Center Blvd Sarasota FL 34240 9258 Tel 941 371 1003 Fax 941 377 5428 E mail sales wpiinc com UK 1 Hunting Gate Hitchin Hertfordshire SG4 OTJ Tel 44 0 1462 424700 Fax 44 0 1462 424701 E mail wpiuk wpi europe com Germany Zoss
23. ur to reach a stable baseline current of 16 80nA before it is used for measurement 7 Ifthe stabilized baseline value exceeds 80nA see Replacing the Membrane Sleeve on page 10 NOTE This polarization procedure assumes the temperature is 25 C At 37 C the baseline current is higher Calibration Procedure The oxygen sensor in combination with a monitoring device amperometrically measures the concentration of oxygen in aqueous solutions and can be used short term 2 hours or less in a gas mixture Once the sensor is polarized It can be calibrated The polarized sensor should already be immersed in filling solution and plugged into the monitoring device The following example briefly describes the fundamental concepts behind a standard calibration protocol Known concentrations of O2 are generated by saturating the solution with oxygen 100 and nitrogen 0 Dry air or a solution saturated with air has a 20 8 concentration of oxygen Using these three known points a calibration curve can be generated NOTE For additional sensor calibration procedures and calibration theory refer to the free radical analyzer manual Most WPI manuals can be downloaded directly from www wpiinc com This information can also be e mailed when you contact the WPI Technical Support team at 941 371 1003 or technicalsupport wpiinc com NOTE For accurate results the sensor must be calibrated at the same temperature expected at the measurement site
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