Optional Column Selection Valve
Contents
1. OutletValve V4 Cond monitor OutletValve V4 SampleWalve V5 BufferYalve 1 V6 FlowDirectionvalve Y7 Valve V8 Add gt gt lt lt Remove Auxiliary Equipment Cancel Help From the Component list select the optional column valve you have installed by checking the box or by clicking ADD Click OK twice and then Close M ark thetwo PV 908 valves 2 and 3 Check thatthe ID codeswitch is set to 2 and 3 respectively AKT design Optional Column Selection Valve User M anual 71 5029 20 Edition AA 15 6 Installation A 9 Mount the brackets and attach the two valves to the system rack see chapter 5 10 Cut and mount the new i d 0 50 mm or i d 0 75 mm capillaries M ount one capillary between port 1 on column valve 2 and port 1 column valve 3 This is the column bypass position used as default position for the valves in UNICORN 16 AKT Adesign O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Operation 7 Operation 7 1 Preparing for operation 1 A connected valve is automatically recognized by UNICORN at system start up Check that the added valve is indicated in the flow schemein UNICORN 2 Verify that the valve is functioning properly by issuing manual commands from UNICORN as follows In System Control select Manual FlowPath Select instruction ColumnPosition Select and execute a new position for the valve View the valve in theflow scheme panel to confirm that the pos2. 3 UNPACKING nern LO A Pre r QuiStteS ein LE 5 Mounting of brackets 12 Bad Valve ID ais sn nn Rat Cas astra dai Sa EE 13 5 2 UniNet Connection ini 14 6 Instalando dora LD 6 1 Preparation for use rrenan 15 7 OHErAHON tai L7 7 1 Preparing for Operation nie ie 17 7 2 AKTAfpic AKTApurifier AKTAbasic UNICORN 3 2 3 21 18 7 3 AKTAfplc AKTApurifier AKTAbasic UNICORN 4 0 4 12 20 KTA design Optional Column Selection Valve User M anual 71 5029 20 Edition AA V Contents vi AKT Adesign O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Introduction GE 1 Introduction 1 1 General AKTA design standard system configurations can be changed to optional configurations This flexibility in AKTA design system strategies allows the user to enhance already used purification methods and also to develop new more complex methods When optional equipment is connected to a standard chromatographic system new sets of instructions to control the optional equipment become accessible for the user O ptional configurations are selected installed and put into operation by the user An optional configuration consists of both hardware components and software instructions O ptional configurations are monitored and controlled via methods run by theUNICORN control system in the same way as AKTA design standard system configurations 1 2 Optional column selection valve Column
3. AsMain 0 00 AveragingTimeUV 2 60 UV_Averaging_time W 0 00 Block Initial Eluent Conditions 0 00 End block 0 00 Block Column Equilibration 0 00 Block Flowthrough Fractionation 0 00 Block Sample Injection 0 00 Block Wash Out Unbound Sample 0 00 Block Eluate and Peak Fractionation 0 00 Block Gradient Elution 0 00 Block Clean After Gradient 0 00 Block Reequilibration 0 00 End method E E EEE CLLLLLLL Click here to define a variable name for the instruction parameter Breakpoint Positi m EN ss InjectionValve Ta Insert el Flowpath InjectionMark cmon ne E Var Change AlarmstMon RSI C Frac lake Watch Other Delete Ready I num 3 Click the Flowpath radio button 4 From the Instructions list select ColumnPosition The valve instruction parameter Position allows you to change all eight positions of the column selection valves synchronously KTA design O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Operation E 5 Definea variable name for the instruction parameters e g ColumnValves Pos This variable allows you to synchronously control the position of the column valves to the position you want Variable name definition Variable name Columrivalves Pos Cancel Clear Help 6 Click OK The defined variable is inserted in the method 7 Return to the Variables page by selecting View Run setup to set the variable ColumnVa
4. In a domestic environment this product may cause radio interferencein which case the user may be required to take adequate measures Terms and Conditions of Sale Unless otherwise agreed in writing all goods and services are sold subject to the terms and conditions of sale of the company within the A mersham Biosciences group which supplies them A copy of these terms and conditions is available on request Should you have any comments on this product we will be pleased to receive them at Amersham Biosciences AB SE 751 84 Uppsala Sweden Trademarks Drop Design KTA KTAFPLC KTA purifier AKTAbasic and UNICORN aretrademarks of A mersham Biosciences Limited A mersham and A mersham Biosciences are trademarks of Amersham plc Office Addresses Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences Limited Pollards Wood N ightingales Lane Chalfont St Giles Bucks H P8 4SP UK Amersham Biosciences Corp 800 Centennial Avenue P O Box 1327 Piscataway N J 08855 USA Amersham Biosciences Europe GmbH M unzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences K K Sanken Building 3 25 1 H yakunincho Shinjuku ku Tokyo 169 0073 Japan Copyright Amersham Biosciences 2003 All rights reserved Contents Contents 1 INHOAUCHON al FI General anna 7 1 2 Optional column selection valve n2urennennennnennennnennernennnnn 1 A ainda Dl SAO asa o 9
5. O AKTA design Optional Column Selection Valve User Manual Amersham 71 5029 20 V Biosciences Important user information All users must read this entire manual to fully understand the safe use of AKTA design instruments WARNING The Warning sign highlights an instruction A that must be strictly followed in order to avoid personal injury Be sure not to proceed until the instructions are clearly understood and all stated conditions are met Caution TheCaution sign is used to call attention to instructions or conditions that must be followed to avoid damage to the product or other equipment Be sure not to proceed until the instructions are clearly understood and all stated conditions are met Note TheN otesign is used to indicateinformation important for trouble free and optimal use of the product CE Certification This product meets all requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked Amersham Biosciences instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from Amersham Biosciences except for alterations described in this manual WARNING This is a Class A product
6. and media screening can be automated by adding two motorized valves onto the system This setup allows Automated method optimization and multi step purification as well as column selection Up to eight columns can be connected onto the system Column performance can be easily compared while changing conditions in a run The setup is time saving and increases reproducibility of the results e Preset protocols will prevent over pressurization and protect columns from breaking KTA design Optional Column Selection Valve User M anual 71 5029 20 Edition AA 7 ED Introduction Column Selection Valve V2 On line filter Restrictor Fraction Collector Column Selection Valve V3 Theoptional column selection valve code no 18 1108 41 is available for KTAFPLC with UNICORN Software 3 2 or higher KTApurifier with UNICORN Software 3 21 AA or higher AKTAbasic with UNICORN Software 3 21 AA or higher 8 AKT Adesign O ptional Column Selection Valve User M anual 71 5029 20 Edition AA 2 Safety gt gt 2 1 Safety The components are designed for indoor use only e Donotusein a dusty atmosphere or close to spraying water e Operate in accordance with local safety instructions WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING Ensure that the entire chromatogr
7. aphic system has been flushed thoroughly with distilled water before removing any capillaries or components WARNING Always disconnect the power supply before attempting to replace any item on the equipment during maintenance WARNING If there is a risk that large volumes of spilt liquid may penetrate the casing of the equipment and come into contact with the electrical components immediately switch off the chromatographic system and contact an authorized service technician CAUTION Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables KTA design Optional Column Selection Valve User M anual 71 5029 20 Edition AA EB Unpacking 3 Unpacking Unpack the optional components and check against the supplied packing list Inspect the items for obvious damage that may have occurred during transportation CAUTION Read the following information carefully as well as all the additional instructions supplied with the components to ensure that the AKTAdesign optional equipment is installed correctly 10 AKT Adesign O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Pre requisites EN 4 Pre requisites The general procedures for creating and editing methods are described inthe UNICORN User Manuals Note The sy
8. ition change has occurred Manually run the system pump with distilled water at a flow rate of approximately 2 ml min and check that water is coming out from the valve port selected Flow Scheme ES Valve panel ren Fe Connect a capillary to the valve port selected on column valve 2 for stop Connect this capillary to the same port on column valve 3 Check that water is coming out from the center port of column valve 3 KTA design Optional Column Selection Valve User M anual 71 5029 20 Edition AA 17 Operation 72 AKTAFPLC AKTApurifier AKTAbasic UNICORN 3 2 3 21 18 7 2 1 Using the column selection function in a run The column selection valves are designated ColumnValves in the flow scheme and V2_ColPos and V3_ColPos in the run data windows To use the column selection valves in a method proceed as follows 1 In the Main Menu select File New Method to create a new method Select System Technique Template and For column Click OK 2 Select View Text instructions to display the text instruction editor Double click on the instruction block where you want to add the column selection instruction e g Start Conditions zz Highlight the instruction below to insert the valve instruction 4 Method Editor UNTITLED MES File Block View Adviser Help oa ala SH Bl E Main 0 00 Base CY 0 10 Column ml Any E W 0 00 Block Start Conditions Start Conditions 0 00 Base Same
9. l 71 5029 20 Edition AA 13 ED Mounting of brackets 14 5 2 UniNet Connection Both the UniN et 1 and the UniN et 2 chain can be interrupted anywhere betw een the system pump and the termination plug to interconnect the optional components in the chain The termination plug can be moved to the last component furthest away from the system pump if motivated by cable routing considerations To PC controller board UniNet 2 chain Fraction collector Termination plug PC running UNICORN Use the attached UniN et 2 cables to connect the valves to the UniN et 2 communication link The valves can be connected anywhere betw een the system pump and the termination plug Check that all capillary and electrical connections are carried out correctly AKT Adesign O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Installation GH 6 Installation 6 1 Preparation for use To start your KTA design optional configuration system 1 Switch on the KTA design instrument with the mains switch located at the front left on the system base Check that the computer and printer are switched on Log in see KTAFPLC AKTApurifier or KTA basic M aking your first runs respectively In UNICORN Main Menu select Administration System Setup Select System and then click Edit Click Component Component x Component selected Component list
10. lves_Pos to the desired position 8 Save the method 19 KTA design Optional Column Selection Valve User M anual 71 5029 20 Edition AA Operation 73 AKTAFPLC AKTApurifier AKTAbasic UNICORN 4 0 4 12 7 3 1 Using the column selection function in a run To use a valve in a method select the desired valve options in the M ethod Wizard when creating the new method The column selection valves are designated ColumnValves in the flow scheme and V2 ColPos and V3 ColPos in the run data window 20 AKT Adesign O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Amersham e Biosciences TC information Uppsala Printed in Sweden by TK i Uppsala AB A
11. stem configuration delay volume must berecalculated and reset when the standard configuration after the UV flow cell is changed KTArFPLc AKTApurifier and KTA basic respectively must be installed and fully tested before the optional components are installed See the Installation G uide for your AK TAdesign chromatography system WARNING Ensure that the entire system has been flushed thoroughly with distilled water before removing any capillaries or components CAUTION Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables AKT A design Optional Column Selection Valve User M anual 71 5029 20 Edition AA 11 ED Mounting of brackets 5 Mounting of brackets Inserting components Removing components 12 CAUTION Make sure that the AKTAdesign instrument is switched off before installing the optional components The mains power to the AKTAdesign instrument must be switched OFF before disconnecting or connecting the UniNet 1 and UniNet 2 cables Optional equipment can be mounted on the system When selecting mounting location for the optional equipment it is important to choose a location which minimize the length of the capillaries used to connect the optional components to the rest of the system M any components that are a
12. ttached to the mounting rails uses a snap in bracket The bracket is supplied separately with the component and needs to be fitted as shown below before the component can be attached 2 Fit the attached mounting bracket with the two screws KTA design O ptional Column Selection Valve User M anual 71 5029 20 Edition AA Mounting of brackets GI IV PV 908 The column selection valve has a unique ID code which identifies it to the UNICORN control system at system start up This ID code should be checked before installation The ID code for the column selection valve and the corresponding instructions used in UNICORN to control it are as follows Valve function ID code Valve type Instruction in UNICORN Column selection 2 PV 908 ColumnPosition combined instruction Column selection 3 PV 908 controlling both valves Components are connected to theUNICORN control system using either UniN et 1 or UniN et 2 cables Both the UniN et 1 and the UniN et 2 data communication chain in standard configuration is routed from the rear of the system pump via their respective components to the last component in the chain where it is terminated with a plug Note When Pump P 960 is used it is always installed as the last component in the UniN et 2 chain Since the pump has an internal termination no termination plug is needed AKT Adesign Optional Column Selection Valve User M anua
Download Pdf Manuals
Related Search