Hoefer SE1210 Gel Drying Frame
Contents
1. Gel turns cloudy or white Gels gt 0 75 mm thick with Reduce the percentage of ethanol to 30 acrylamide 10 T Do not soak the gel for more than 2 hours in the etha nol glycerol dehydration solution After Coomasie Blue staining be sure to use final destain of 7 acetic acid and 5 methanol before soaking in 35 ethanol glycerol solution p8 7 Ordering information product quantity code no Replacement Parts 20 cm x 20 cm frames and platform Cellophane sheets 33 cm x 33 cm 50 SE1202 Mylar sheets 33 cm x 33 cm 25 SE1204 Gel drying frame 1 SE1210 20 cm x 20 cm Includes inner and outer frames 8 black knobs and one O ring sealing gasket Order loading platform separately O ring sealing gasket 20 cm x 20 cm frame 1 SE1212 Knobs black 10 SE1213 Gel loading platform 20 cm x 20 cm 1 SE1214 Outer gel drying frame 1 SE1215 and 8 knobs 20 cm x 20 cm Inner gel drying frame il SE1216 20 cm x 20 cm and one O ring sealing gasket Replacement Parts 21 cm x 26 cm frames and platform Cellophane sheets 33 cm x 38 cm 50 SE1202 L Mylar sheets 33 cm x 38 cm 25 SE1204 L Gel drying frame 1 SE1210 L 21 cm x 26 cm Includes inner and outer frames 8 black knobs and one O ring sealing gasket Order loading platform separately O ring sealing gasket 21 cm x 26 cm frame 1 SE1212 L Knobs black 10 SE1213 Gel loading platform 21 cm x 26 cm 1 SE1214 L Outer g2. for the bottom and one sheet of either porous cellophane or polyester for the top Cellophane Use for fluorography thick gels or to preserve a gel Cellophane does not quench the signal of fluor treated gels Polyester Use for contact autoradiography for gels lt 1 mm The polyester peels off once the gel is dried Place the uncovered side against the X ray film Fully hydrate cellophane sheets in distilled water This will take several minutes Polyester sheets do not require hydration Unlock the frame knobs and gently pop the inner frame out of the outer frame Position the inner frame so that the side labeled UP faces up If using the loading platform ordered separately see Fig 1 on page 1 for the platform and inner frame positions 4 Place a fully hydrated sheet of cellophane over the inner frame on the loading platform if available Pour 5 10 ml of distilled water on the sheet Assemble the drying stack Place the gel s on the cellophane Once all the bubbles are removed pour 5 10 ml of distilled water on the gel as well as around the edges Fig 2 Locking the frames together 0 Cover the gel with a sheet of polyester or fully hydrated cellophane Displace any remaining bubbles working from the center of a single gel or if several gels are in place from the center of the frame toward the edge Position the outer frame with the Hoefer label fa
3. the frame with facing up repacking should it become necessary to return the screws facing the unit down Assembly All eight knobs are installed into the outer frame until they fit just short of snug Once installed these knobs are adjusted by a 1 4 turn to lock or to unlock the frame See Fig 2 on page 5 Cellophane 2 EN AIS Inner frame with the a gt word UP facing up ae Loading platform ordered separately Note This drying method is NOT recommended for e Agarose gels which are gener ally too thick e Polyacrylamide gels attached to treated polyester film 2 Instructions Listed below are recommended procedures to ensure optimum drying results for gels of differ ent thicknesses and acrylamide concentrations Thin gels 0 75 mm or less are recommended for best results Prepare the gel for drying Thin gels Gel thickness lt 0 75 mm or gel gt 0 75 mm and acrylamide lt 10 T 0 Fix and stain the gels according to your preferred protocol Final soak solution Coomassie Blue The final destaining solution should consist of 7 acetic acid 5 methanol and 0 5 2 glycerol Glycerol maintains the flexibility of the gel making it less likely to crack Equilibrate the gel in this solution for a minimum of 2 hours but preferably overnight After destaining rinse the gel with distilled water for 1 2 minutes to remove excess acetic acid Silver stain Add 0
4. usen manual Hoefer SE1210 Gel Drying Frame For air drying gels um SE1210 IM Rev HO 08 12 A AHoeter Page finder 1 Gel Drying Frame function Unpacking sissioni Assembly nsss 2 INSHPUCHIONS aaa yana anaa Prepare the gel for drying 3 Gel Drying Stack Assembly 4 Drying GelS ccceceeeeeee Air Drying ccccecceeeeeen eee After Gels are Dry 5 Care and maintenance Cleaning sasaka aa sawan Changing the Gasket 6 Troubleshooting 7 Ordering information and description 1 ee Gel Drying Frame function and description The Hoefer SE1210 gel drying frames hold gels between cellophane and or heavy gauge polyes ter plastic sheets for drying in a rack placed in a fume hood Each gel frame consists of an inner and outer frame eight black knobs and one installed O ring sealing gasket Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all Fig 1 Frame parts and gel items arrived If any part is missing contact drying stack assembly your local sales office Inspect all components for damage that may have occurred while the Screw all 8 knobs into unit was in transit If any part appears damaged the threaded holes in b Hee ia Outer frame contact the carrier immediately Be sure to keep tals all packing material for damage claims or for When assembling Hoefer label h orient
5. 5 2 glycerol to the final water rinse Fluorography Follow the manufacturer s instructions or your preferred protocol Load the gel onto the drying frame as described on page 4 Note Unless gel cracking is a recurring problem prevent gels from becoming tacky by using lt 2 glycerol gel soaking solution Note f you soak gels in a solution of ethanol and glycerol you can dry the gels after the preserve step is completed Thick gels Gel thickness gt 0 75 mm and acrylamide gt 10 T 0 Fix stain and destain the gel according to your preferred protocol Use a final destaining solution of 7 acetic acid and 5 methanol Final soak solution Coomassie Blue After the final destain soak the gel in a solution of 35 ethanol and 0 5 2 glycerol for 1 2 hours Ethanol dehydrates the gel which mini mizes cracking during drying Do not place the gel in the ethanol solution longer than 2 hours as ethanol will begin destaining the gel Silver stain Soak gels in 30 ethanol 5 glycerol for at least 30 min prior to drying Fluorography Follow the manufacturer s instructions or your preferred protocol Load the gel onto the drying frame as described on page 4 Important Remove all bubbles from the gel Push them care fully toward the edge of the gel with gloved finger 3 Gel Drying Stack Assembly 0 Select the drying sheets You need one sheet of porous cellophane
6. cing up and the knobs facing down around the inner frame Press down to engage the two frames Apply ing pressure to opposite corners of the frame usually causes the frames to snap together easily Both upper and lower sheets should be taut Turn the assembly over to turn each knob 1 4 turn into the locked position as shown in Fig 2 The long edge of the knob bridges the inner and outer frames Locking the two frames together prevents slippage as the gel shrinks during drying Closed 4 Drying Gels Air Drying Place the frames in a suitable rack preferably in an operating fume hood If gels positioned vertically are found to slide toward the bottom reposition them and dry them horizontally Slip ping can be prevented by carefully blotting off excess water before clamping the frame closed After Gels are Dry 0 Remove the gels from the frames Cellophane Trim excess wrapping if desired Polyester For contact autoradiography peel plastic off before exposing the gel to X ray film Rinse frames thoroughly with water and then distilled water after each use Important Never autoclave or heat any component of the frame above 55 C 5 Care and maintenance Cleaning Clean the frame regularly with mild soap and water rinse thoroughly with water and distilled water and allow to air dry Never use abrasive detergents Do not expose the frame to solutions or vapors of aromatic or hal
7. el drying frame 1 SE1215 L and 8 knobs 21 cm x 26 cm Inner gel drying frame 1 SE1216 L 21 cm x 26 cm and one O ring sealing gasket p9 Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc Coomassie is a trademark of ICI ple 2012 Hoefer Inc All rights reserved Printed in the USA Hoefer
8. ogenated hydrocar bons ketones esters alcohols over 30 or concentrated acids over 25 Changing the Gasket If the gasket on the inner frame becomes inflexible or no longer holds the gel stack tautly remove it and install a new gasket stretching it slightly around three sides and then rolling it into place on the fourth 6 Troubleshooting problem solution Gel or cellophane cracks All gels Handle the gel and cellophane with care Fully hydrate the cellophane with distilled water just before use and avoid stretching it during assembly If the cellophane is drying too quickly instead of hydrating it in distilled water soak it in the same glycerol solution used for the gel Always remove all air bubbles before drying Gradient gels or gels Dehydrate the gel in 30 ethanol and 2 glycerol gt 0 75 mm thick with before drying If gels still crack increase the percentage acrylamide 10 T of glycerol up to 5 Note With a higher percentage of glycerol a longer drying period is required The gels may also be flexible and feel tacky when dry Fluor treated gel Use cellophane instead of polyester Use a thinner gel lt 0 75 mm Gel dries too slowly or not at all Gel dries unevenly Rotate each frame 180 front to back every half hour Check the gel or stack Reduce the amount of glycerol to 0 5 2 Check that you are not using two sheets of polyester At least one sheet must be cellophane
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