E-Gel™ PowerBase
Contents
1. Go button and Insert the power plug into the electrical outlet and then connect the cable to the iBase unit Release the Go button and connect the iBase to this computer with a USB A to B cable A into the computer B end into the iBase The computer should now begin to search for the iBase This step may take several minutes The program will inform it is searching for the iBase The program will inform the iBase has been found Please press Next to begin the iBase Firmware Update Please do not disconnect or use device until iBase Updater is complete The update was successful Please disconnect the USB cable from the iBase device TM The iBase device is now updated Trouble In case a message The Update Failed Retry the shooting program and if the problem persists contact Technical Support for further assistance 34 Explanation of Symbols and Warnings EN60825 1 A Caution Sq WEEE The CE mark symbolizes that the product conforms to all applicable European Community provisions for which this marking is required The E Gel iBase Power System and E Gel Safe Imager Real time Transilluminator comply with the Underwriters Laboratories Inc regulation and the European Community Safety requirements The E Gel iBase Power System and E Gel Safe Imager Real time Transilluminator comply with part 15 of the FCC rules The E Gel Safe Im2. Outlet Mode Go eooee Note The fan in the device operates constantly during an electrophoresis run However when the power is on but there is no electrophoresis run the fan operates only upon demand of the electronic components Continued on next page Device Installation Continued Installing the E Gel Safe Imager Trans illuminator Alone Place the E Gel Safe Imager Real time Trans illuminator on a level bench with enough space around the unit to allow air circulation and prevent overheating Plug the connecting end of the power cord with the transformer a into the back inlet of the Safe Imager and connect the power cord to the electrical socket The short electrical cord b remains disconnected To Power a Outlet Short electrical cord stays disconnected A steady red light illuminates the E Gel Safe Imager Real time Transilluminator indicating that it is properly connected and ready to use 19 Running the iBase Power System Selecting Programs 20 Before inserting the E gel cassette make sure the program you want to use is set up properly 1 Press the Mode button M until the program field a blinks D E Gel iBase En 2 Select the appropriate program for the gel type using the Up Down A Y buttons to change the program see page 21 for list of programs 3 To change the run time press the Mode button until the minutes or
3. are damaged contact in the base is intact due to improper use Expired or defective Use fresh gel cassette Use gel cassette used properly stored gels before the specified expiration date Gel cassette is not Remove cassette and reinsert a correctly inserted into steady red light is illuminated on the base the base when the cassette is correctly inserted and power is on iBase not properly Plug the E Gel Safe Imager connected to E Gel Real time Transilluminator short Safe Imager Real time electrical cord into the iBase Transilluminator power inlet directly Over run Accidentally selected Select program according to the gel or an incorrect program program parameters on page 21 need more If you are at the beginning of the ep to run run stop the run and select the gel desired program If you are well into the run check the gel to see where the loading dye is running Estimate the amount of time remaining and manually stop the run 32 Continued on next page Troubleshooting Continued Problem Reason Solution Failure Mode Defective cassette Disconnect iBase and indicated by remove the gel cassette from Cassette Missing the base Hold Go to Reset Press and hold the Go button and continuous for 2 seconds to return to loud beeping Ready Mode Use a fresh gel cassette Cold cassette Use a room temperature cassette stored at room temperature Avoid storing gel cassettes
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5. during run Up Down buttons green Count down time A V 30 E Gel Safe Imager Quick Reference Guide Introduction A quick reference guide for operating the E Gel Safe Imager Real time Transilluminator is provided below Mode Action LED Indicator Light Safe Imager Safe Imager connected to an electrical steady red light plugged in outlet and ready to use Light on for 30 Press and release ON OFF button flashing green ligh seconds Light on for 5 press and hold the ON OFF button for steady green minutes a few seconds followed by a flashing green light the last 30 seconds of the run Light turned off press and release the ON OFF button Steady red light Error Wait until the LED turns a steady red flashing red LED before turning on the device again If the LED does not turn red after the run disconnect the Safe Imager and try again after a few minutes If this problem persists please contact the Invitrogen Technical Support 38 31 Troubleshooting Introduction The table below provides some solutions to the problems you might encounter when using iBase or E Gel Safe Imager Real time Transilluminator For troubleshooting resolution and sample preparation problems refer to the manual supplied with the gels Problem Reason Solution No current Copper contacts in the Make sure that the copper iBase
6. electrical outlet A separate stand alone power supply is not required to run the iBase E Gel iBase Power System back view Power inlet USB port Continued on next page 13 Overview Continued E Gel Safe Imager Real time Trans illuminator E Gel Safe Imager Features 14 The E Gel Safe Imager Real time Transilluminator is designed for viewing E Gel with SYBR Safe gels and E Gel CloneWell gels on the laboratory bench top for real time monitoring on the E Gel iBase Power System or for documentation purposes at the end of the run directly on the E Gel Safe Imager The E Gel Safe Imager Real time Transilluminator has the following features e An array of 12 LED sources behind a blue filter that emit high intensity blue light e Ared ON OFF button located at the front e 30 seconds and 5 minutes automatic shut off options e A LED indicator light just behind the ON OFF button to indicate the status of the Safe Imager e A short electrical cord to connect to the iBase e USB port to enable future program updates E Gel Safe Imager Real time Transilluminator top Light source LED Indicator light ON OFF button E Gel Safe Imager Real time Transilluminator back USB port Power inlet Attached short electrical cord Continued on next page Overview Continued Emission Spectrum of E Gel Safe Imager Light from
7. electrode connections on the E Gel iBase device Press on the left side of the cassette to secure it into the iBase The two electrodes on the right side of the gel cassette must be in contact with the two electrode connections on the base see page 22 The LED illuminates with a steady red light to show that the cassette is correctly inserted 4 Select the program PRE RUN 2 min and press the Go button to pre run the gel The LED light changes to green indicating that the cassette is in the pre run mode 5 After two minutes the pre run stops automatically as indicated by a red light and a beeping sound 6 Gently remove the comb s from the gel 7 Proceed to Running the Gel Step 4 page 22 Continued on next page Running the iBase Power System Continued Downstream You are now ready to proceed to imaging or any other application with the gel Application Interrupting a Run View SYBR Safe stained gels and E Gel EX and SizeSelect agarose gels as described in Using E Gel Safe Imager Real time Transilluminator page 27 Remove the E Gel cassette from the iBase and view gels using your own imaging system For E Gel CloneWell gels refer to the QRC for further running information You can interrupt an electrophoresis run at any time by pressing and releasing the Go button to stop the current The stopped current is indicated by a flashing red light and the digital display flash
8. 8 E Gel 1 2 with SYBR Safe 18 gels G5218 01 E Gel 2 with SYBR Safe 18 gels G5218 02 E Gel 0 8 with Ethidium Bromide 18 gels G5018 08 E Gel 1 2 with Ethidium Bromide 18 gels G5018 01 E Gel 2 with Ethidium Bromide 18 gels G5018 02 E Gel 4 with Ethidium Bromide 18 gels G5018 04 1 2 Clear E Gels 18 gels G5518 01 E Gel 0 8 double comb with Ethidium Bromide 18 gels G6018 08 E Gel 2 double comb with Ethidium Bromide 18 gels G6018 02 E Gel Sample Loading Buffer 4 x 1 25 ml 10482 055 E Gel 25bp DNA Ladder 100 apps 10488 095 E Gel 50bp DNA Ladder 100 apps 10488 099 E Gel 1Kb Plus DNA Ladder 100 apps 10488 090 Molecular The recommended marker for each gel type is listed in Weight the manual supplied with the gels or the E Gel Markers Technical Manual For more information visit www invitrogen com or contact Technical Support page 38 38 Technical Support Web Resources Visit the Invitrogen website at www invitrogen com for e Technical resources including manuals vector maps and sequences application notes MSDSs FAQs formulations citations handbooks etc e Complete Technical Support contact information e Access to the Invitrogen Online Catalog e Additional product information and special offers Contact Us For more information or technical assistance call write fax or email Additional international offices are listed on our website www inv
9. E Gel k OERA 5 invitrogen 6 If you want to view the bands with the E Gel Safe Imager Real time Transilluminator while the gel runs place the Amber filter unit on top of the gel and view the gel as described in Using E Gel Safe Imager Real time Transilluminator page 27 7 To start electrophoresis press the Go button a green light illuminates to show that the run is in progress The LCD displays the count down time while the run is in progress 8 The run will stop automatically when the programmed time has elapsed The iBase signals the end of the run with a flashing red light and rapid beeping for 30 seconds followed by a single beep every minute The LCD displays Run Complete Press Go 9 Press and release the Go button to stop the beeping The light turns to a steady red light and the LCD display shows the last selected time and program Continued on next page 23 Running the iBase Power System Continued Pre Runs 24 The PRE RUN program is used prior to running any of the following types of E Gel cassettes e _ E Gel 0 8 1 2 2 4 e E Gel double comb e E Gel CloneWell Do not use the PRE RUN program for E Gel EX or E Gel SizeSelect cassettes To pre run a gel follow these instructions 1 Select the PRE RUN program on the iBase device 2 Open the package and remove the gel Do not remove the comb s until step 6 3 Slide the cassette into the two
10. EU G6465EU G6500STEU G6400UK G6400STUK G6465UK G6500STUK E Gel iBase leach 1each leach 1 each Power System E Gel CloneWell 18 cassettes 18 cassettes 0 8 SYBR Safe E Gel Safe leach 1 each 1 each Imager Real time Transilluminator E Gel High Range 100 appl DNA Ladder Continued on next page Product Contents Continued Component G6511ST G6512ST G6612ST G6511STEU G6512STEU G6612STEU G6511STUK G6512STUK G6612STUK E Gel iBase Power 1 each 1 each 1 each System E Gel EX 1 E Gel EX 2 E Gel SizeSelect 2 E Gel Safe Imager Real time Transilluminator 50 bp DNA Ladder E Gel 1 Kb Plus DNA Ladder 10 cassettes 1 each 100 appl 10 cassettes 100 appl 10 cassettes 1 each 50 ug Upon Receiving Instrument damage Examine the unit carefully for any damage incurred during transit Any damage claims must be filed with the carrier The warranty does not cover in transit Product Specifications E Gel iBase The specifications for the E Gel iBase Power System Specifications are listed below Dimensions 18 4 cm x 11 cm x 5 75 cm Weight 500 g Electrical Requirements 100 240V AC 50 60Hz 1 0 A Temperature Ambient 5 C to 40 C Built in Features LCD Display Alarm LED light Adapter Specifications Use only the UL Listed original adapter supplied Input 100 240
11. Safe stained E gels Imager agarose gels The iBase fits neatly on the Real time Integrated Transilluminator and power is provided through a System shared power cord adapter included with the E Gel 16 iBase Power System With the matching amber filter mounted on top of the iBase included with the E Gel Safe Imager Real time Transilluminator you can follow the migration of DNA bands while they are running or document your results at the end of the run directly iBase and Safe Imager Integrated System E Gel iBase U 7 Power System Amber Filter E Gel Safe Imager Real time Transilluminator Device Installation Installing the E Gel iBase Power System and E Gel Safe Imager Trans illuminator Follow these instructions to install the E Gel iBase Power System with the Safe Imager Real time Transilluminator 1 Place the E Gel Safe Imager Real time Trans illuminator on a level bench with enough space around the unit to allow air circulation and prevent overheating 2 Place the iBase directly onto the E Gel Safe Imager Real time Transilluminator so that the legs of the iBase fit directly into the grooves of the Safe Imager 3 Plug the short electrical cord of the E Gel Safe Imager Real time Transilluminator a into the power inlet of the iBase b To Power Outlet 4 Plug the connecting end of the powe
12. VAC 50 60Hz 1A Output 48 VDC 0 8 A min Continued on next page Product Specifications Continued E Gel Safe Imager Specifications TM The specifications for the E Gel Safe Imager Real time Transilluminator are listed below Viewing surface dimensions 62 mm x 77 mm Case dimensions 200 mm x 110 mm x 43 mm Amber filter dimensions 121 mm x 138 mm x 31 mm Weight of Safe Imager 243 g Weight of Filter 55g Electrical Requirements 100 240VAC 50 60Hz 1 0A Temperature Ambient 5 C to 40 C Built in Features LED light LED life 50 000 hours LED Specifications Array of 12 high power LEDs emitting at 480 5 nm The LEDs used radiate less than 10 Lumens each at 200 mA Included accessories Amber filter unit and viewing glasses for viewing results Adapter Specifications Use only the UL Listed adapter supplied with the starter kit or with the E Gel iBase Power System Input 100 240 VAC 50 60Hz 1A Output 48 VDC 0 8 A min Continued on next page Product Specifications Continued Use Conditions The E Gel iBase Power System and E Gel Safe Imager Real time Transilluminator comply with the Underwriters Laboratories Inc regulation and the European Community Safety requirements Operation of the E Gel iBase Power System and E Gel Safe Imager Real time Transilluminator are subject to the following conditions e Indoor use e Altitude below 2 000 mete
13. ager Real time Transilluminator complies with AS NZS CISPR22 04 Australia The product adheres to the EN60825 1 standard publication date EN 60825 1 1994 Al A2 Operation of the E Gel iBase Power System and E Gel Safe Imager Real time Transilluminator are subject to the conditions described in this manual The protection provided by the equipment may be impaired if the equipment is used in a manner not specified by Invitrogen The Caution symbol denotes a risk of safety hazard Refer to accompanying documentation The WEEE Waste Electrical and Electronic Equipment symbol indicates that this product should not be disposed of in unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of WEEE Visit www invitrogen com weee for collection and recycling options The E Gel Safe Imager Real time Transilluminator is classified as a Class 1 LED product which is indicated by the symbol to the left 35 Explanation of Symbols and Warnings Continued A yellow label is affixed to the side of the E Gel Safe Imager Amber filter saying Caution Class 2 LED radiation when open do not stare into the beam 36 Purchaser Notification Limited Use Label License No 5 Invitrogen Technology The purchase of this product conveys to the buyer the non transferable right to use the purchased amount of th
14. at 4 C Improper operating conditions TM Use iBase at room temperature 20 C to 25 C Bands not visible in middle area of gel Gel overheated due to selection of improper time or program Let gel cool down and check again Use suggested settings next time High background suboptimal or no image when using Safe Imager No filters or wrong filter set Refer to E Gel Technical Guide to determine the optimal filter sets to use or contact the instrument manufacturer for advice Photographic settings not optimal Optimize settings of your system empirically for E Gel with SYBR Safe or other blue light excitable stains see page 15 You may need to increase the exposure time or gain setting Low sample concentration Use E Gel EX or E Gel SizeSelect agarose gels Stripes visible on image when using Safe Imager No IR coating on camera lens Use IR blocking filter or emission filter with IR coating 33 Downloading Firmware Upgrades Firmware Instructions are provided below to upgrade the Update firmware on the E Gel iBase Power System 1 Download the iBase updater program from 10 11 12 www invitrogen com ibase Disconnect the electrical plug of the iBase from the electrical outlet Please make sure the USB cable is not connected Press and hold the Go button red button Continue holding the
15. claim against the buyer of infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon the manufacture use or sale of a therapeutic clinical diagnostic vaccine or prophylactic product developed in research by the buyer in which this product or its components was employed provided that neither this product nor any of its components was used in the manufacture of such product If the purchaser is not willing to accept the limitations of this limited use statement Life Technologies is willing to accept return of the product with a full refund For information about purchasing a license to use this product or the technology embedded in it for any use other than for research use please contact Out Licensing Life Technologies 5791 Van Allen Way Carlsbad California 92008 or outlicensing lifetech com 37 Accessory Products Additional Additional products available separately from Products Invitrogen are listed in the table below For more information visit our website www invitrogen com or contact Technical Support page 38 Product Quantity Catalog no E Gel EX 1 10 Pak 10 gels G4010 01 E Gel EX 1 20 Pak 20 gels G4020 01 E Gel EX 2 10 Pak 10 gels G4010 02 E Gel EX 2 20 Pak 20 gels G4020 02 E Gel EX 4 10 Pak 10 gels G4010 04 E Gel SizeSelect 2 10 Pak 10 gels G661002 E Gel CloneWell 0 8 SYBR Safe 18 gels G6618 0
16. duct has been damaged by accident abuse misuse or misapplication or from ordinary wear and tear This warranty shall be limited to the replacement of defective products It is expressly agreed that this warranty will be in lieu of all warranties of fitness and in lieu of the warranty of merchantability Invitrogen a part of Life Technologies Corporation warrants that E Gel Safe Imager Real time Transilluminator will be free from defects in material and workmanship for a period of one year from date of purchase If a defect is present Invitrogen will at its option repair replace or refund the purchase price of this product at no charge to you provided it is returned during the warranty period This warranty does not apply if the product has been damaged by accident abuse misuse or misapplication or from ordinary wear and tear This warranty shall be limited to the replacement of defective products It is expressly agreed that this warranty will be in lieu of all warranties of fitness and in lieu of the warranty of merchantability 2009 Life Technologies Corporation All rights reserved For research use only Not intended for any animal or human therapeutic or diagnostic use NOTES 41 NOTES 42 Corporate Headquarters Invitrogen Corporation 1600 Faraday Avenue sbad 760 Ta F 1 760 602 65 E tech servi vitrogen com For country specific contact information visit our web site at www invitrogen
17. e product and components of the product in research conducted by the buyer whether the buyer is an academic or for profit entity The buyer cannot sell or otherwise transfer a this product b its components or c materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes The buyer may transfer information or materials made through the use of this product to a scientific collaborator provided that such transfer is not for any Commercial Purpose and that such collaborator agrees in writing a not to transfer such materials to any third party and b to use such transferred materials and or information solely for research and not for Commercial Purposes Commercial Purposes means any activity by a party for consideration and may include but is not limited to 1 use of the product or its components in manufacturing 2 use of the product or its components to provide a service information or data 3 use of the product or its components for therapeutic diagnostic or prophylactic purposes or 4 resale of the product or its components whether or not such product or its components are resold for use in research For products that are subject to multiple limited use label licenses the terms of the most restrictive limited use label license shall control Life Technologies Corporation will not assert a
18. e the bands migrate less than half the length of the gel A run exceeding 7 minutes under these conditions results in a defective run The SPEED E Gel mode is not compatible with E Gel 4 cassettes 21 Running the iBase Power System Continued Running the For instructions on sample preparation refer to the Gel E Gel manual supplied with the gels or the E Gel Technical Guide ci Select the program and set the time for your run see page 20 If you need to pre run the gel follow the instructions on page 24 Open the package and remove the gel Gently remove the comb s from the gel Slide the cassette into the two electrode connections on the E Gel iBase device Press on the left side of the cassette to secure it into the iBase The two electrodes on the right side of the gel cassette must be in contact with the two electrode connections on the base The LED illuminates with a steady red light to show that the cassette is correctly inserted Slide cassette into electrodes Press left side to secure Select the appropriate program to run your cassette according to the table on the previous page 22 Continued on next page Running the iBase Power System Continued Running the Continued from previous page Gel continued 5 Load your samples as directed by the E Gel manual Be sure to load the appropriate molecular weight markers and add water to any empty wells _ iBase
19. ed to a mains socket outlet with matching protective earthing connections Mains plug is a disconnect device and must be easily accessible Do not touch or handle the E Gel cassette during a run when the green light is illuminated Do not attempt to open the E Gel Safe Imager Real time Transilluminator The adapter must be connected to a mains socket outlet with matching protective earthing connections Mains plug is a disconnect device and must be easily accessible Use of controls or adjustment or performance of procedures other than those specified herein may result in hazardous radiation exposure Class 2 LED radiation when open Do not stare into the beam Always use the E Gel Safe Imager amber filter unit or E Gel Safe Imager viewing glasses provided with this device while viewing gels to protect your eyes Amber filters absorb radiation from 190 530nm with an OD of over 3 The E Gel Safe Imager Real time Transilluminator is classified as a Class 1 LED product which is indicated by the symbol to the left A yellow label is affixed to the side of the E Gel Safe TM Imager Amber filter saying Caution Class 2 LED radiation when open Do not stare into the beam Continued on next page 9 Safety Information Continued Detailed Safety Information E Gel Safe Imager 10 The E Gel Safe Imager Real time Transilluminator is an electrical device Neve
20. es to indicate that the run was interrupted The display also shows Press GO to Run Hold Go to Reset You can remove the gel from the iBase to check the progress of the run Then To continue the run from the point at which it was stopped reinsert the gel and press and release the Go button The light changes to steady green and the LCD display shows the count down time It is also possible to change the remaining run time but not the program as described on page 20 before continuing the run To cancel the rest of the interrupted run press and hold the Go button for a few seconds The LCD display will reset and the base will return to Ready Mode If desired you can then select a new program or run time as described on page 20 and rerun the gel Continued on next page 25 Running the iBase Power System Continued Running in Reverse Direction Note Maintaining the E Gel iBase 26 The REVERSE E Gel program is used to run E Gel agarose gels in a reverse direction This is particularly useful for isolating fragments using E Gel CloneWell and E Gel SizeSelect agarose gels 1 Toggle between program minutes and seconds by pressing the Mode button M until program blinks 2 Select the REVERSE E Gel Program using the Up Down A Y buttons to change the program 3 If you want to change the run time press the Mode button until the minutes or seconds blink and change the values usin
21. es when excising bands from DNA gels ON OFF button Always use the E Gel Safe Imager Amber filter unit or E Gel Safe Imager viewing glasses they help to visualize DNA when using blue light excitable stains and also prevent Caution prolonged exposure of your eyes to the intense blue light 2 Switch the E Gel Safe Imager Transilluminator on using the ON OFF button in one of these ways e To activate the light for 30 seconds press and release the ON OFF button The LED indicator light flashes green throughout the run e To activate the light for 5 minutes press and hold the ON OFF button for a few seconds The LED indicator light turns a steady green then turns to flashing green in the last 30 seconds of the run DNA stained with blue light excitable stains see page 15 is immediately visible when the light is on and amber filter unit or viewing glasses are in place 3 To turn off the light press and release the ON OFF button The LED indicator light turns red Note A flashing red LED indicates an error Wait until the LED turns a steady red before turning on the device again If the LED does not turn red after the run disconnect the Safe Imager and try again after a few minutes If this problem persists contact the Invitrogen Technical Support page 38 Continued on next page 27 Using E Gel Safe Imager Continued Documenting To document your results you may use any standard Re
22. g the Up Down buttons the maximal run time for reverse running is 3 minutes 4 To start electrophoresis press the Go button a green light will illuminate to show that the run is in progress The LCD display will show the count down time while the run is in progress 5 The iBase will signal the end of the run with a flashing red light and rapid beeping for 30 seconds followed by a single beep every minute while the LCD display will read Run Complete Press GO 6 Press and release the Go button to stop the beeping The light turns to a steady red light and the LCD display shows the last selected time and program 7 Remove the E Gel cassette from the iBase You are now ready to proceed to imaging or any other application with the gel We recommend that you disconnect the E Gel iBase Power System from the electrical outlet when not in use for a prolonged period of time Keep the surfaces of the E Gel iBase Power System free of contaminants To clean disconnect from power source and wipe with a dry cloth Do not attempt to open or service the bases To honor the warranty bases should only be opened and serviced by Invitrogen Using the E Gel Safe Imager Introduction Instructions to view gels with the E Gel Safe Imager Real time Transilluminator are described in this section Viewing 1 Place the Amber filter unit on top of the sample as Gels shown below or use the viewing glass
23. h water and mild soap or ethanol Avoid damaging or scratching the glass surface of the Safe Imager with abrasive cleaners sharp instruments or harsh solvents Before cleaning the instrument disconnect it from the electrical outlet We recommend that you disconnect the E Gel Safe Imager Real time Transilluminator from the electrical outlet when not in use for a prolonged period E Gel iBase Quick Reference Guide Introduction A quick reference guide for operating the E Gel iBase Power System is provided below Mode Action Sound LED Light LCD Display Base iBase Fan working Yellow light Default program plugged in connected to for 3 seconds for3seconds RUN E Gel or an electrical if a cassette is last program outlet not inserted used or red light if a cassette is inserted Ready with Gel cassette Steady red Default program no current inserted into or last program flowing a base used through gel Pre run Press and Steady green Program name release the and count down Go button time End of Pre Automatic Beeping Flashing red Run Complete run sound Press Go and count up time Run Press and Steady green Program and release the count down time Go button End of run Automatic Continuous Flashing red Run Complete beeping for Press Go and 30 seconds count up time followed by a single beep every minute Pause Press and Flashing red Program name ma
24. iBase e Program selection option Features e Time control e Reverse mode running option e High power capability e LCD display e USB port to enable future program updates Continued on next page 11 Overview Continued E Gel iBase Description Down button The E Gel iBase Power System has an LCD display which shows information about the program selected and running time The display is located near the upper edge of the iBase Just below the display the E Gel iBase Power System has four buttons see image below e A Go button to start programs e A Mode button to toggle between programs minutes and seconds e An Up button marked A to select between programs on the display and increase running time e A Down button marked W to select between programs on the display and decrease running time A LED light is located in the middle of the four buttons which indicates the status of the iBase The gel cassette is inserted into the two electrode connections at the lower half of the iBase E Gel iBase Power System top view Up button 12 Continued on next page Overview Continued E Gel iBase Description Continued from previous page At the back the E Gel iBase Power System contains a USB port and a power inlet The supplied power cord has a matching connector that inserts into the power inlet and connects the E Gel iBase Power System to the
25. invitrogen E Gel iBase Power System E Gel Safe Imager Real time Transilluminator General information for using the E Gel iBase Power System with firmware version 1 4 0 Catalog nos G6400 G6400EU G6400UK G6400ST G6400STEU G6400STUK G6465 G6465EU G6465UK G6500 G6500ST G6500STEU G6500STUK G6511ST G6511STEU G6511STUK G6512ST G6512STEU G6512STUK G6612ST G6612STEU G6612STUK Rev Date 4 August 2009 Part no 25 0951 MAN0000573 Table of Contents Table of Content ccsssssssssssecscscsessssssssssessssssssesesesesecesssssnsnsnsesessessssseseseees 3 Product Contents ssesesesesenenenensnennonensnsnsnenenenenenssnnnsnsnsnenenenenensnnnensnenennnenee 4 Product Specifications eesesesenesnonensnnnsnenenenenenssnnnsnsnsnenenenensnsnnnnenenennnnnnnene 6 Safety Information esesesessesesenessnssnensnnnenssenenenenensnnsnsnsnsnnnnnnssnenenenennsnennnnenen 9 Overview eienares aa AEA ESLA Een ASERNE DADAS PENNA ES ASER ESAS aN ARAE ESEAS 11 Device Installation ssssesesesesesosssnssensnenenenenenonononsnsnsnenenenenensnnnnnnnnsnennnenenene 17 Running the iBase Power System ususssnsssensnsenensnsensnsnnsnsnssnensnnenensnnenene 20 Using the E Gel Safe Imager cs sssssenssussssnsiorssnsesonsvensswesetsonensteansncsconeseenes 27 E Gel iBase Quick Reference Guide 2u0200002020200002000002000200 0000000000000 29 E Gel Safe Imager Quick Reference Guide uesssssnsesnsn
26. itrogen com Corporate Headquarters Japanese Headquarters European Headquarters 5791 Van Allen Way LOOP X Bldg 6F Inchinnan Business Park Carlsbad CA 92008 USA 3 9 15 Kaigan 3 Fountain Drive Tel 1 760 603 7200 Minato ku Tokyo 108 0022 Paisley PA4 9RF UK Tel Toll Free 1 800 955 6288 Tel 81 3 5730 6509 Tel 44 0 141 814 6100 Fax 1 760 602 6500 Fax 81 3 5730 6519 Fax 44 0 141 814 6117 tech_support invitrogen com jpinfo invitrogen com eurotech invitrogen com MSDS Material Safety Data Sheets MSDSs are available on our website at www invitrogen com msds Certificate of The Certificate of Analysis provides detailed quality control Analysis and product qualification information for each product Certificates of Analysis are available on our website Go to www invitrogen com support and search for the Certificate of Analysis by product lot number which is printed on the box Continued on next page 39 Technical Support Continued iBase Warranty Safe Imager Warranty 40 Invitrogen a part of Life Technologies Corporation warrants that E Gel iBase Power System will be free from defects in material and workmanship for a period of one year from date of purchase If a defect is present Invitrogen will at its option repair replace or refund the purchase price of this product at no charge to you provided it is returned during the warranty period This warranty does not apply if the pro
27. n UV transilluminators Although the viewing glasses do block UV light they are not designed for use as UV safety glasses Do not leave the E Gel Safe Imager Real time Transilluminator switched on for extended periods of time After viewing and documenting the gel or sample always switch the unit off Overview Introduction In this section we first describe the following devices e E Gel iBase Power System which is designed to run E Gel single comb double comb CloneWell TM EX and SizeSelect cassettes e E Gel Safe Imager Real time Transilluminator designed to view gels run on the iBase that use the SYBR Safe DNA gel stain or E Gel EX and SizeSelect gels that use a proprietary blue light excitable fluorescent nucleic acid stain Other blue blue light excitable stains visible with the E Gel Safe Imager include SYBR Gold SYBR Green I and II SYPRO Ruby SYPRO Orange and Coomassie Fluor Orange Together these systems form an integrated system for running and viewing SYBR Safe stained E gel agarose gels E Gel The E Gel iBase Power System is an easy to use iBase programmable automated device designed to simplify Power electrophoresis of single comb or double comb E Gel System cassettes from Invitrogen The E Gel iBase Power System is a base and a power supply combined in one device E Gel The E Gel iBase Power System offers
28. nually release the with or and remaining end the run Go button without count down time during the cassette alternating with run Press Go to Run Hold Go to Reset Restart after Press and Steady green Program and manual stop release the count down time Go button Continued on next page 29 E Gel iBase Quick Reference Guide Continued Mode Action Sound LED Light LCD Display Return to Press and hold the With gel Last program and Ready mode Go button cassette in time setting after a steady red manual stop Without gel cassette no light No cassette Last program used Backlight turns off after 3 minutes No cassette Press Go button Cassette not detected Timer setting Press the Mode With gel Time increases by button until the cassette minutes or 10 minutes or seconds steady red seconds blink and change the Without gel values using the cassette no Up Down buttons light A V Program Press the Mode With gel Selected program setting button M until the cassette program blinks steady red Select the appropriate Without gel program for the gel cassette no type using the light Up Down A buttons Remove Without pressing the Rapid Flashing red Cassette Missing cassette Go button beeping Hold Go to Reset during run or failure detected Time change Change time using Steady Program and
29. r cord with the transformer c into the back inlet of the Safe Imager and connect the power cord to the electrical socket A steady red light illuminates the E Gel Safe Imager Real time Transilluminator indicating that it is properly connected and ready to use The fan in the iBase begins and the LED yellow and LCD are activated indicating the device is on The fan and LED will turn off after 3 seconds if no gel is inserted The LCD initially displays the firmware version for a few seconds then changes to display the default program PRE RUN 2 minutes or the last program used Continued on next page 17 Device Installation Continued Installing the E Gel iBase Power System Alone 18 1 Verify that the power cord supplied with the unit is compatible with the local socket format Contact Technical Support page 38 if the plug does not fit Plug the connecting end of the power cord with the TM transformer a into the back inlet of the iBase then plug the other end to an electrical outlet Use only properly grounded AC outlets and power cords The fan in the device begins and the LED yellow and LCD are activated indicating the device is on The fan and LED will turn off after 3 seconds if no gel is inserted The LCD initially displays the firmware version for a few seconds then changes to display the default program PRE RUN 2 minutes or the last program used To Power
30. r touch the power cord or outlet with wet hands Do not use this device in damp areas or while standing on damp floors Do not attempt to open the E Gel Safe Imager Real time Transilluminator TM The E Gel Safe Imager Real time Transilluminator should be used with the power cord supplied your starter kit or with the E Gel iBase Power System This power cord has a universal transformer compatible with 90 V to 220 V Only these power cords should be used to power the device Attach the power cord to the E Gel Safe Imager Real time Transilluminator at the back of the device Plug the other end of the power cord into a properly grounded electrical outlet ensuring the correct plug adaptor is attached Always disconnect the E Gel Safe Imager Real time Transilluminator from the electrical outlet before cleaning the device The E Gel Safe Imager Real time Transilluminator does not produce UV light however it does utilize an intense blue light for viewing gels It should be noted that published literature has identified blue light as a possible risk factor for macular degeneration however no clinical studies have been published Therefore the E Gel Safe Imager amber filter unit or E Gel Safe Imager viewing glasses provided with this device should be used to protect your eyes while viewing gels The amber filter unit is NOT a safety screen for UV emission and will NOT protect your eyes when viewing gels o
31. rs e Temperature range 5 to 40 C e Maximum relative humidity 80 e Installation categories over voltage categories II Pollution degree 2 e Mains plug is a disconnect device and must be easily accessible e Do not attempt to open the iBase or Safe Imager device To honor the warranty iBase and Safe Imager device can only be opened and serviced by Invitrogen e The protection provided by the equipment may be impaired if the equipment is used in a manner not specified by Invitrogen e The device must be connected to a mains socket outlet with protective earthing connections e Ventilation requirements no special requirements The E Gel iBase Power System and E Gel Safe Imager Real time Transilluminator comply with part 15 of the FCC rules Operation of the devices are subject to the following conditions e The device may not cause harmful interference e The device must accept any interference received including interference that may cause undesired operation Ethrog Biotechnologies Ltd an Invitrogen company is the manufacturer and owner of the UL file For more information contact Technical Support page 38 or Ethrog Ethrog Biotechnologies Ltd 12 Hamada St P O Box 4035 Rehovot Israel 74103 Safety Information A Caution a For iBase Caution For Safe Imager TM Do not attempt to open the E Gel iBase Power System The adapter must be connect
32. seconds fields blink Change the values using the Up Down buttons Do not exceed the maximal run time for the program see page 21 Note To reset the iBase Power System press and hold the Go button for three seconds until the display reads E Gel iBase Continued on next page Running the iBase Power System Continued Program The E Gel iBase Power System firmware version 1 4 Parameters is pre programmed with 10 different programs for running various types of E Gel cassettes Refer to the table below for the run parameters default time and maximum allowable time for each program Program Gel Type Default Maximal Time Time 0 PRE RUN E Gel 0 8 1 2 2 4 E Gel double comb E Gel CloneWell 2 min 2 min 1 E Gel 0 8 2 E Gel 0 8 1 2 2 26 min 40 min 2 E Gel 4 E Gel 4 30 min 40 min 3 E Gel DC E Gel double comb 13 min 20 min 4 CloneWell 0 8 E Gel CloneWell 12 min 60 min 5 Reverse E Gel E Gel CloneWell E Gel SizeSelect 2 min 3 min 6 Speed E Gel E Gel 0 8 1 2 2 7 min 7 min 7 E Gel EX 1 2 E Gel EX 1 2 10 min 20 min 8 E Gel EX 4 E Gel EX 4 15 min 20 min 9 SizeSelect 2 E Gel SizeSelect 8 min 20 min Speed Runs The SPEED E Gel program is used to perform a quick run to obtain a yes no result The program utilizes high power and is only suitable for 0 8 1 2 and 2 E Gel cassettes This program is limited to 7 minutes wher
33. sssssensnsnsnene 31 Troubleshooting esesesesesesesesnsnsnsnsnsnenenenenenonononsnsnsnenenenenensnnnnnnenenensnenenene 32 Downloading Firmware Upgrades esesesessesenssenensnenenenenensnnnnnnsnenenenenenene 34 Explanation of Symbols and Warnings sssssssesesesessonononsnsnsesnnnensnenenenenen 35 Purchaser Notification cccssssssssssssssececccssssssssssesesesssssessesesesesesesssnssensess 37 Accessory Products ccccccsssseseesssssssesssessssssssssesesesssesesssesssssesessesesessseseees 38 Technical Suppott esesesesesesesessssenenensneneneneessononsnsnsnsnenenenenensnnnnnnnsnensnenenene 39 Product Contents Types of This manual is supplied with the following products Products Product Catalog no G6400 E Gel iBase Power System G6400EU G6400UK E Gel Safe Imager Real time Transilluminator G6500 Device and Amber Filter E Gel iBase and E Gel Safe G6465 Imager Combo Kit G6465EU US EU UK versions G6465UK E Gel EX 1 Starter Kit a US EU UK versions or G6511STEU E Gel EX 2 Starter Kit a US EU UK versions Sol TUR G6512STEU E Gel SizeSelect 2 Starter Kit rn US EU UK versions ea G6612STUK E Gel Safe Imager Real time G6500ST Transilluminator Starter Kit for G6500STEU Cloning US EU UK versions G6500STUK Contents The contents of the products are listed below Component G6400 G6400ST G6500 G6465 G6500ST G6400EU G6400ST
34. sults Maintaining the E Gel Safe Imager Note 28 imaging device Due to the small footprint the E Gel Safe Imager Real time Transilluminator may fit inside the cabinet of your current gel documentation system The documentation can be performed with or without the iBase unit on the Safe Imager In many cases satisfactory results are obtained by placing the amber filter unit on top of the gel and photographing imaging as normal The distance between the camera and the gel may have to be adjusted In addition some CCD documentation systems may include a filter that will work in place of the amber filter unit contact the manufacturer for filter specifications Please refer to the E Gel Technical Guide for instructions on best documentation for E Gel with SYBR Safe gels or the proprietary blue light excitable fluorescent nucleic acid stain in E Gel EX and SizeSelect agarose gels If you wish to document gels with other stains compatible with the E Gel Safe Imager Real time Transilluminator see page 15 please refer to the directions on imaging conditions and filters in the instruction manual of the relevant stain Make sure to switch the E Gel Safe Imager Real time Transilluminator on before documenting the results After viewing or documenting the results switch the E Gel Safe Imager Real time Transilluminator off The Safe Imager can be cleaned with a dry cloth a wet cloth wit
35. the array of 12 LED sources inside the transilluminator passes through a blue filter producing a single intensity signal at approximately 480 nm effective for the excitation of the SYBR Safe DNA gel stain and many of our other nucleic acid and protein stains such as SYBR Gold SYBR Green I and II SYPRO Ruby SYPRO Orange Coomassie Fluor Orange and the proprietary blue light excitable fluorescent nucleic acid stain used in E Gel EX and TM SizeSelect gels Unlike UV transilluminators the E Gel Safe Imager Real time Transilluminator does not produce UV light however it does utilize an intense blue light for viewing gels Therefore the E Gel Safe Imager filter unit or E Gel Safe Imager viewing glasses provided with this device should always be used to protect your eyes while viewing gels amber Note The amber filter unit is NOT a safety screen for UV emission and will NOT protect your eyes when viewing gels on UV transilluminators And although the viewing glasses do block UV light they are not designed for use as UV safety glasses Emission spectrum for the E Gel Safe Imager Real time Transilluminator 350 400 450 500 550 600 Wavelength nm Continued on next page 15 Overview Continued E Gel E Gel iBase Power System and E Gel Safe Imager iBase and Real time Transilluminator form an integrated system E Gel Safe for running and viewing SYBR
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