05068-800-DU002-A - Cryolys User Manual
Contents
1. USER MANUAL gt cryolys Lape advanced temperature controller bertin TECHNOLOGIES Introduction CRYOLYS INDEX DE Ol Sn engraved AESI EEEa aiaia 3 1 1 Safety information and recommendations 3 1 2 MNT OA CY sete suvetaas tans cane sabe tenten de ee idee T E T TT 6 1 3 Appliance reference details ccccccccccsscssssseeeeeeeeeecessseeeeeesecenssseeeeeesseeeasseeeeeeesseaeenssseeeeess 6 1 4 Manufacturer s address ccccccccssssssceeecceeceessseeeeeccesceaseeeeeceeeeensseeeeesesseeasaseeeeeeesseeasnseeeeeeenseas 6 1 5 TechNical SUN DO a ee das ee 6 2 Description of the CRYOLYS 00 0 eccccceccccceeceeeeeeeeeeeeeceeeeesaeseeeeeneeenanes 7 2 1 Product OV CL VICW sccaccrwiaaciacnneassaiedunescsn saametaronnmansiiaatsnesanatnsmmasiedsnmnnasesaietegesseanetwoaeustaneaawiatncaceaes 7 2 2 Generating cold conditions iii 8 2 3 Sotaa a e ce E E E E E E A E E A E aces E S E E ZEEEEZEZ 9 2 4 CONTO PN eae EEE E EE N EE E 9 2 5 Technical Specifications eeeessceeecceeecesssseeeeceeeecessseeeeeeseeasssseeeecesseeanseeeeesesseeagsseeseeeeetees 10 3 Transport NS PRET NEO DT EE D ni 11 3 1 WIE SID OIE E AE E O E O E E E E 11 3 2 AG e A A A ee ee en ee ne ee 12 E O a a a TE 13 4 1 TMD a lt ia e I a E E PE OTE E E E EEE E de 13 4 2 MASE eel ONE and CONNEC ES a E ON 15 Os UTS CS a a a a eo 19 Dad Start Up OC SIS a ne da ee ne nt 19 5 2 Preparation of the cooling system 0 0 ecsscececcceecesssseeeeeceseeeessseeeeeceeenseeeeeeeesse2. 3 Set up the controls on the PRECELLYS 24 so that the next touch on the Valid key starts the cycle see PRECELLYS 24 User Manual 4 Close the PRECELLYS 24 lid Select the outflow on CRYOLYS using the adjustment button at the front WARNING Do not apply pressure greater than 10 bars 145 PSI otherwise the internal components of the cooling system may deteriorate 5 3 Running a cycle 1 At the end of the start up phase re adjust the amount of liquid nitrogen in the tank so that the highest spiral of the serpentine is immersed The nominal consumption of nitrogen is 1 litre for 5 minutes of operation Put the cover on the CRYOLYS Put the switch in the ON position so that the led is on The cold air is diffused over the tubes 4 Press the PRECELLYS 24 Valid button when the displayed temperature on the thermometer reaches 10 C The cycle will start Page 19 24 Instructions for use WARNING Do not to open the PRECELLYS 24 cover while the switch is on or the cooling system is being supplied with air 5 At the end of a PRECELLYS 24 cycle turn the CRYOLYS switch to the OFF position 6 Open PRECELLYS 24 s lid and take out the tubes using gloves 5 4 Running a sampling sequence If the last use was less than 4 hours ago release operations from chapter 5 2 Preparation of the cooling system Start up procedure is not necessary If the last test finished more than 4 hours ago restart ope
3. lift it up by the copper coil or by the flange of the tank The unit must be handled using the slots located at the bottom and on the side of the shell Slots are also available on the protective foam Page 13 24 Installation CRYOLYS 4 Check the external parts of the unit Any defect should be reported immediately to the delivery company 5 Keep the original packaging protective foam and manual these items will be required for transport An adapter or appropriate lead not supplied will be needed for use with any non standard European electrical plug Page 14 24 Installation 4 2 Installation and connection When purchasing a PRECELLYS 24 and CRYOLYS unit at the same time see chapter entitled 4 2 2 Pneumatic connection When a CRYOLYS unit is purchased to complement a PRECELLYS 24 follow the instructions described in chapter entitled 4 2 1 Installation of the diffusion bowl 4 2 1 Installation of the diffusion bowl e Unpack the diffusion bowl Unscrew the 2 screws of the PRECELLYS 24 cover using a n 6 Allen key N 3 Remove the original cover 4 Position the diffusion bowl WARNING Use the 2 provided screws only Screw at a torque of 2 Nm the 2 screws on the cover using a dynamometric key Switch on the PRECELLYS 24 Start a grinding cycle Open the cover slightly and check that the PRECELLYS 24 stops to check the correct Top Alarm release CON O U
4. not tilt the equipment the CRYOLYS must always stand on its four feet Failing to do so could damage its internal components break its plastic shell or cause liquid nitrogen to be spilt Do not operate the unit when the shell is partially or entirely removed or when it is damaged Do not install unauthorised cards components or accessories as this may alter the protection given by the appliance and cancel the warranty Do not overfill the tank with nitrogen see 5 1 Start up procedure Do not transport the unit before emptying the nitrogen tank completely Do not transport the unit in packaging which is different from the original one Do not supply the unit with air pressure which is different from the specified one Do not use gas which is different from the specified one Do not use a power adapter which is different from the one supplied with the CRYOLYS Page 3 24 Introduction 1 Bertin Technologies is not responsible for any damage or injuries which may occur as a result of operating the unit in a way other than that specified in this document 1 3 Biological risks Individual protective equipment IPE must be worn when cleaning parts which have been contaminated by biological samples for instance the inside of the diffusing bowl See PRECELLYS 24 user manual 1 4 Risks associated with liquid nitrogen Figure 1 Risk of asphyxiation Figure 2 Risk of burns Figure 3 Protect
5. CELLYS tubes holder can be partially or entirely equipped with tubes The main advantages of CRYOLYS It keeps samples at a temperature which is below 30 C during homogenization It protects temperature sensitive molecules from degrading It is compatible with the lysis and homogenization product PRECELLYS 24 The CRYOLYS cooling system is as follows Nitrogen tank Diffusion bowl Outflow selector ON OFF Switch CRYOLYS cover Stainless steel flex Electrical connector connected Location of temperature probe N EEE Air outflow Air inflow Page 7 24 Description of the CRYOLYS 2 2 Generating cold conditions Cold air is generated continuously and independently of the PRECELLYS 24 working cycles Figure 6 Operating process 1 Clean dry air cools as it travels through the spiral 2 Cold air is diffused through the specific cover Temperature Profile with Cryolys Option 6800rpm 3x30s 30s break O oO O1 oO Temp in tubes aN O emp in tubes w Cryolys Temperature Precellys Run on off wo oO Time minutes Figure 7 Example of temperature curve in tubes with and without Cryolys Page 8 24 Description of the CRYOLYS 2 3 Settings The user can opt for one of three different air flow rates by activating the front Switch The user will determine the optimal air flow rate to use acc
6. T WARNING When the unit is ON do not stop the PRECELLYS 24 by opening the cover Page 15 24 Installation WARNING Please keep the packaging Also please keep the foam protection This protection must be re used for transporting the equipment Should a unit be returned to the distributor or manufacturer without this protection the warranty will be void 4 2 2 Pneumatic connection Dry air is required to use the CRYOLYS unit purity 99 99 water content lt 5 Connection of the supply pipe with air from the cooling system 1 Use an air supply pipe adapted to employed pressure pipe not provided Fix a clamp on to the air supply pipe not provided Fix the pipe onto the grooved connection external 10 mm so that the connection is completely covered 4 Position the collar in the middle of the connection Firmly tighten the collar with a screwdriver Connection of the cooling systems s air outlet to the PRECELLYS 24 diffusion bowl 1 Position the CRYOLYS and the PRECELLYS 24 so that the flexible tube can connect them without being too tight or slack 2 Unscrew the adaptator at the other end 3 Screw the adaptator on the air intake of the diffusion bowl Page 16 24 Installation j 4 Screw the flexible tube on the connection and tighten it with a n 22 wrench The user should use another wrench to secure the fixed parts 4 2 3 Electrical connection Connect the CRYOLYS u
7. eeeaasseeeeess 19 5 3 RUNNING Ay OA E E E E O sib neundenes 19 5 4 Running a sampling sequence iii 20 5 5 PAC O Cy O a ee A 20 6 Maintenance Preventative maintenance ssesssesssrrserrrerrrerrrerrresrres 21 6 1 TOUDE SOC A E ae ae en ee a 21 6 2 Cleaning and decontamination iii 22 Page 2 24 Introduction 1 Introduction This user manual contains all the information required on unpacking installing operating and maintaining the CRYOLYS The technical specifications of the product and the following information may change without prior notice 1 1 Safety information and recommendations User manuals for the CRYOLYS cooling system and PRECELLYS 24 must be read carefully by the user before operating the product In case of any doubt about the safety of the equipment please contact the local distributor 1 1 1 Risk of electric shock Although the appliance is perfectly safe users must be aware of the potential risks associated with handling liquids near an electric power supply In the event of a liquid being accidentally spilt the appliance must be immediately switched off by unplugging the main power lead even if the appliance is running The appliance must then be dried and the spilt liquid wiped up Do not plug the appliance in until it has been checked 1 1 2 Incorrect operation Operating the equipment in other ways than those detailed in this user manual may alter the protection given by the appliance Do
8. ely evaporated 4 Ensure that there is no dampness especially around the power connection Otherwise dry the damp areas with a dry cloth Page 11 24 Transport Storage l CRYOLYS 3 2 Storage The equipment must be stored in a dry area at a temperature of between 0 C and 50 C on its four feet The equipment can be stored after checking the following 1 The tank is empty 2 The power lead is unplugged 3 The equipment is dry clean and decontaminated Page 12 24 Installation CRYOLYS 4 Installation WARNING Do not connect the unit to the main supply before installation has been carried out Do not tilt the unit The cooling system should always rest on its four feet Failure to do so could damage the internal components or the plastic shell 4 1 Unpacking 1 Check the contents of the box is as follows 1 user manual French English 1 CRYOLYS cooling system bowl 1 diffusion bowl with 2 screws 1 mains lead and adaptator 1 thermometer probe and display 1 AC power cord 1 EC certificate 1 manufacturer s inspection certificate Should some parts be missing when delivered please contact your local distributor immediately 2 Take out the diffusion bowl thermometer and power lead 3 Take the CRYOLYS carefully out of its box and install it on a clean horizontal stable worktop near the PRECELLYS 24 WARNING When removing the unit from its box do not
9. f the There is no voltage 2 Check the lead between the unit and switch does not the mains come on 1 Turn off the power The Led is defective 2 Contact technical support Electrical power One or several outflows do not work One or several electro valves ere detective Contact technical support 1 Wait f iffusi de icing The diffusion holes are ait for diffusion bowl de icing obstructed 2 If malfunction persists contact TOWE technical support performance of Th tfi moval the system ourmow Contro valves are Contact technical support disturbed The thermometer is defective Contact technical support Page 21 24 Maintenance Preventative maintenance 6 2 Cleaning and decontamination 6 2 1 Recommendations The external cover of the appliance can be cleaned with a sponge or wet cloth which has been soaked in water or alcohol WARNING For safety reasons and so as not to damage the appliance please follow the following recommendations Do not allow the equipment to be splashed or sprinkled with fluids particularly the electrical connector Cleaning must always be carried out with the appliance disconnected Do not use a sponge scraper as this may damage the equipment Do not use acetone or sodium Do not subject the appliance to an air decontamination process 6 2 2 Example of decontamination procedure Should splashes occur the parts likely to be contamina
10. he appliance is operated intensively frequent filling of liquid nitrogen Successive cycles frost can block the air exit holes on the cover and prevent proper cooling of the tubes In such cases the nominal operational mode is no longer guaranteed Page 5 24 Introduction CRYOLYS 1 2 Warranty Bertin Technologies certifies that this product is free from defects at time of shipment This warranty is limited to a period of one 1 year This warranty does not cover the following circumstances If the unit has not been installed operated or maintained according to the instructions described in this user manual If the unit has been repaired or modified by unauthorised personnel If the unit s serial number has been damaged or removed 1 3 Appliance reference details Appliance code CRYOLYS 05068 200 RD000 1 4 Manufacturer s address BERTIN TECHNOLOGIES Parc d activit s du Pas du Lac 10 bis avenue Amp re 78180 Montigny le Bretonneux France Tel 33 0 1 39 30 60 00 Fax 33 0 1 39 30 61 85 e mail precellys bertin fr 1 5 Technical support Should a problem occur please contact the local distributor Page 6 24 Description of the CRYOLYS CRYOLYS 2 Description of the CRYOLYS 2 1 Product overview The CRYOLYS is an appliance whose purpose is to cool biological samples during the PRECELLYS lysis and homogenization in order to protect them from excessive heating The PRE
11. ion of eyes and face Figure 4 Protection of hands and skin Liquid nitrogen is a colourless odourless highly refrigerated gas around 196 C The main risks associated with the handling of this product are asphyxiation and burns To protect against projections and asphyxiation risks the operator must wear equipment which protects the eyes face and skin store and use the liquid nitrogen in an aerated location It is advised to have the safety instructions to hand about the risks and precautions associated with the utilization of liquid nitrogen Page 4 24 Introduction 1 1 5 Risks associated with compressed air 1 Do not disconnect the in and out airflow pipes when they are under pressure Do not apply a pressure of more than 8 bars 116 PSI as this may damage the internal components of the cooling system 1 6 Mechanical risks Figure 5 Risk of pinching The diffusion bowl may close itself It is advisable to hold it open by hand for any operation requiring it to be opened Otherwise it is advised to shut the bowl T 7 j Safety sign Ne pas ouvrir le capot lors du fonctionnement Do not open the cover while running Respect the instructions associated with these signs Only staff authorised by the distributor or the manufacturer are authorised to repair the equipment 1 8 Noise level In normal operating mode the appliance has a noise level below 70 dBA 1 9 Intensive operating mode When t
12. nit to the socket using the power cable provided Page 17 24 Installation CRYOLYS WARNING Do not connect the CRYOLYS unit with a different power cable to the one supplied 4 2 4 Installation of the thermometer 1 Unpack the thermometer 2 Insert the probe into its shell until it emerges in the bowl 3 Fix the probe with the thumb screw 4 Place the display unit on the metal plate of the bowl Probe Probe Thermometer tightening screw Magnetic display Figure 9 Temperature probe positioning inside and outside view Page 18 24 Instructions for use 5 Instructions for use All installation procedures must be carried out before using CRYOLYS 5 1 Start up procedure The start up procedure should to be carried out where the last use of the CRYOLYS was more than 4 hours previously In which case it is important to cool the tank 1 Wear individual protective equipment IPE as previously described 2 Pour enough liquid nitrogen into the tank to immerse the highest spiral of the coil The purpose of this procedure is to cool the tank Put the cover on the tank 4 Wait for the nitrogen to evaporate approx 5 min WARNING Do not inhale nitrogens fumes 5 2 Preparation of the cooling system 1 Check that the switch is in the OFF position and that all connections are correct electrical connection and pneumatic connection 2 Place the number of tubes desired in the PRECELLYS 24 tube holder
13. ording to the reaction to heat of samples as well as the PRECELLYS 24 usage settings Lee Number of cycles Pause time Le Rotation speed Number of tubes 2 4 Control Panel The control panel consists of a knob which can be turned to 3 different positions and an ON OFF switch It is important to select the right position for the outflow before switching on the CRYOLYS Figure 8 Control panel in position 01 OFF Page 9 24 Description of the CRYOLYS 2 5 Technical Specifications Voltage 230 V 50 Hz standard Europe With the adapter supplied Recommended Flexible PVC tube with an internal diameter of 10 mm supporting up to 8 bars 116 PSI of pressure which should be attached using a tightening collar Operating Requirements 70 90 110 min at 20 C and Air outflow 1 ATM Pressure of compressed air From 5 to 10 bars relative source 73 PSI to 150 ame ah COMPESSSSS Purity 99 99 water lt 5ppm Led switched on when ON Switch ON OFF Led switched off when OFF Air supply tube not provided Page 10 24 Transport Storage l CRYOLYS 3 Transport Storage 3 1 Transport Avoid violent shocks that may damage the equipment Before transporting the equipment the following actions should be done 1 Remove the CRYOLYS cover 2 Check that the tank does not contain any liquid nitrogen 3 If there is it should be left open until the liquid nitrogen has complet
14. rations of chapters 5 1 Start up procedure 5 2 Preparation of the cooling system and 5 3 Running a cycle 5 5 End of cycle If you don t want to realize another run 1 Allow the nitrogen to evaporate or suck it up using suitable equipment 2 Wipe out the white frost and water using a cloth flexible tube flange of tank inside of diffusion bowl 3 Switch off the thermometer WARNING Avoid any direct skin contact with the cold parts of the PRECELLYS 24 and the CRYOLYS Do not heat the nitrogen using a hairdryer or similar object to speed up the evaporation process Handle equipment with suitable gloves whilst the tank contains liquid nitrogen Page 20 24 Maintenance Preventative maintenance CRYOLYS 6 Maintenance Preventative maintenance 6 1 Troubleshooting The following table provides a list of the principal problems encountered when the equipment is in operation and the action s to be carried out Problem Possible Cause Action s to be carried out encountered 1 Check the power source There is no voltage 2 Check the lead between the unit and the main The air does not a E 1 Check that the air source is open reach the pee res 2 Check that there is no leak along the St StOn 20M flexible tube 1 Wait for diffusion bowl de icing 2 If malfunction persists contact technical support The diffusion holes are obstructed 1 Check the power source The Led o
15. ted by infectious agents must be decontaminated using a suitable disinfectant The user is solely responsible for choosing the decontamination procedure Parts of the equipment likely to be contaminated may for example be cleaned with a cloth soaked in diluted bleach at 6 Cl Should a different decontamination procedure be required please contact the technical department beforehand in order to check that the procedure is compatible for use with the equipment Page 22 24 Maintenance Preventative maintenance CRYOLYS Manufactured by BERTIN TECHNOLOGIES Parc d activit s du Pas du Lac 10 bis avenue Amp re BP 284 78053 Saint Quentin en Yvelines Cedex France Tel 33 0 1 39 30 60 00 Fax 33 0 1 39 30 61 85 e mail precellys bertin fr User manual code 05068 800 DU002 A Revised SEPT 2007 English Printing DEC 2007 Code equipment CRYOLYS 05068 200 RD000 Page 23 24 Bertin Technologies Parc d activit s du Pas du Lac 10 bis avenue Amp re 78180 Montigny le Bretonneux FRANCE www precellys com precellys bertin fr Tel 33 0 1 39 30 60 00 Fax 33 0 1 39 30 61 69
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