TRC-01M - NPI Electronic Instruments
Contents
1. range x10 range x100 range xl range x10 range x100 range x1 range x10 range x100 range x1 range x10 range x100 range x1 range x10 range x100 249 Q 1 1 ms to 100 us 11 ms to 1 ms 110 ms to 10 ms 110 ms to 10 ms 11 ms to 1 ms 1 1 ms to 100 us 110 us to 10 us 1 1 ms to 100 us 11 ms to 1 ms 11 ms to ms 1 1 ms to 100 us 110 us to 10 us 11 us to 1 us 110 us to 10 us 1 1 ms to 100 us 1 1 ms to 100 us 110 us to 10 us 11 us to I us 19 rackmount cabinet 3U high 1U 1 3 4 44 45 mm version 1 02. are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET am
3. 2 PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only 2 5 System Grounding EPMS 07 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration m sm EPMS E 07 F The 19 cabinet is connected to the CHASSIS connector at the rear panel The CHASSIS is linked to protective earth as soon as the PWR 03D is connected It can be connected also to the SYSTEM GROUND SIGNAL GROUND on the rear panel of the instrument see Figure 3 Dyn Mya r w mportant Always adhere to the appropriate safety measures Figure 3 Rear panel connectors of the EPMS E 07 2 6 Technical Data 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 Power supply 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W EPMS E 07
4. ASS GAIN DEFAULT trim pot 10 turn potentiometer for setting the AMPLITUDE of the SLOW time constant T1 of the transient The AMPLITUDE determines the fraction of T1 from the summed signal at connector 10 6 Tl SLOW HIGHPASS potentiometer 7 T1 HIGHPASS RANGE switch 10 turn potentiometer for setting the time constant of the HIGHPASS filter for the SLOW time constant T1 of the transient The RANGE of this time constant is set by switch 7 Turning the potentiometer clockwise decreases the time constant 10 SUMMING OUTPUT connector BNC connector providing the sum of all time constants and the linear component which will be subtracted from the recorded current signal This signal is not inverted 11 CURRENT HS connector BNC connector for connecting the current output from the voltage clamp amplifier The transient generated by the TRC 01M is subtracted from this signal by the LPBF 01GX in this configuration 11 MONITOR T1 OUTPUT connector BNC connector providing the signal obtained from differentiation of the command signal using the setting of the SLOW time constant T1 12 MONITOR LINEAR OUTPUT connector BNC connector providing the linear component of the transient version 1 0 page 7 TRC 01M User Manual 13 MONITOR FILTERED COMMAND connector BNC connector providing the FILTERED COMMAND signal This signal can be linked to the VOLTAGE COMMAND INPUT of the amplifier see amplifier manual In this case this signa
5. External power supply for EPMS E 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of external power supply W x D x H 225 mm x 210 mm x 85 mm version 1 0 page 5 TRC 01M User Manual 3 TRC 01M 3 1 System Description The TRC 01M was designed as an instrument for compensating capacitive transients in voltage clamp mode which occur when the potential is stepped from a holding potential to a test potential at the beginning and the end of the voltage step In order to do this the voltage clamp pulse is differentiated with three adjustable time constants Tl SLOW T2 MEDIUM and T3 FAST and added to a linear component to compensate for the leakage current The adjustments are made with HIGHPASS and LOWPASS filters and an AMPLITUDE setting for each time constant The COMMAND INPUT can also be filtered by a LOWPASS filter This module is normally used with a differential preamplifier e g npi PA 01GX or a modified LPBF 01GX in order to subtract the signal generated by the TRC 01M from the measured current signal This procedure reduces the capacitive current transients largely and allows it to set the current sensitivity at the amplifier see respective amplifier manual to higher values without saturating the data acquisition Therefore the effective resolution of the recorded signal is better Note The voltage clamp circuit of the amplifier is not affected 1 e the TRC 01M cannot be used to get the voltage clamp faster 3 2 Des
6. cription of the Front Panel O TRC 01M COMMAND LINEAR TI SLOW T2 MEDIUM 100 O 1 HIGHPASS HIGHPASS GAIN A GAIN DEFAULT GAIN DEFAULT AMPLITUDE RANGE o o FILTERED MONITOR MONITOR CURRENT HS MONITOR MONITOR COMMAND LINEAR 11 T 13 3 SUMMING OUTPUT OUTPUT OUTPUT OUTPUT OUTPUT OUTPUT COMMAND INPUT O O Figure 4 TRC 01M front panel view version 1 0 page 6 TRC 01M User Manual The front panel elements for the three time constants T1 T2 and T3 are identical and therefore described only once for T1 1 COMMAND LOWPASS potentiometer 10 turn potentiometer for setting the time constant of the LOWPASS filter for the command voltage range 3 3 ms to 140 us 2 COMMAND AMPLITUDE potentiometer 10 turn potentiometer for setting the AMPLITUDE for the command voltage range 0 to 100 Using this potentiometer the AMPLITUDE of the command voltage can be attenuated in order to avoid clipping 3 LINEAR AMPLITUDE potentiometer 10 turn potentiometer for setting the AMPLITUDE of the linear component of the current caused by command step 4 Tl SLOW LOWPASS potentiometer 9 Tl LOWPASS RANGE switch 10 turn potentiometer for setting the time constant of the LOWPASS filter for the SLOW time constant T1 of the transient The RANGE of this time constant is set by switch 9 Turning the potentiometer clockwise increases the time constant 5 Tl SLOW AMPLITUDE potentiometer 8 Tl LOWP
7. e Np Electronic Instruments for the Life Sciences pAs e uade Te OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE TRC 01M TRANSIENT COMPENSATION MODULE FOR EPMS SYSTEMS TRC 01M HIGHPASS COMMAND GAIN RANGE au BGN e ip kho F a T i J E AN a3 4 AMPLITUDE RANGE i ape MONITOR bond pe RG OUTPUT OUTPUT URRENT HS VERSION 1 0 npi 2007 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com TRC 01M User Manual Table of Contents le Soy RE UI OS dota alan reas redadas 3 2 EPNIS 07 Modular Plugeln SC a esas 4 2 1 General System Description Oper Oi as 4 22s A AA a On A 4 2 Oy INS 0 TAOUSINS A iaiahaaslamndasiuus tearm siubentncan eet 4 DA PW OSD o A 4 2 5 System Grounding iessen a a a a aa E a a 5 A A E A 5 EPMS E O sisas 5 2 05 Tecimical DA aasa r T 5 A E E E E 5 FEMS A E E 5 Je ERC OTN e e nusz dons goccuue aeaceceesneeianc nese dons nocenene sence cmomnentanc nest eees aneemeee ae eece 6 Sal System Descr PUMA a 6 32 Desempuon OF the Front Panta 6 A OPE idol 9 Asli Recommended CONE CUONS s cniiiirer nn cesta ea acacdide A EA 9 4 2 Adjusting the compensati0n CIPFCUIE sanee 9 Da A O ee Er 10 version 1 0 page 2 TRC 01M User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic
8. e shipped with the EPMS 07 housing Y EPMS 07 cabinet with built in power supply Y Mains cord Y Fuse2A 1l A slow Front covers In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing 2 3 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow Front covers SSSSSS The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the power supply 2 4 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from the one of the three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 1 Figure 3 A POWER LED indicates that the PWR 03D is powered on see Figure 1 Power switch voltage selector and fuse are located at the rear panel see Figure 2 Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected version 1 0 page 4 TRC 01M User Manual PWR DID CAEN A DAA E OUA E Figure 1 PWR 03D front panel view Figure
9. l should be monitored in parallel by an oscilloscope in order to control the right COMMAND value when using the COMMAND AMPLITUDE potentiometer see above 14 COMMAND INPUT connector BNC connector for connecting the voltage clamp COMMAND signal Usually this signal 1s also connected to the VOLTAGE COMMAND INPUT of the amplifier version 1 0 page 8 TRC 01M User Manual 4 Operation This TRC 01M is operated in conjunction with a slightly modified TEC 05X no CURRENT FILTER no CURRENT SENSITIVITY switch and a slightly modified LPBF 01GX see also addenda to the respective manuals 4 1 3 Recommended connections Connect a command signal for the voltage clamp e g from the data acquisition system to COMMAND INPUT 15 Figure 4 and in parallel to the VOLTAGE COMMAND INPUT of the amplifier using a branch connection Connect CURRENT OUTPUT FROM HEADSTAGE of the amplifier to CURRENT HS 11 Figure 4 of the TRC 01M Connect the SUMMING OUTPUT 10 Figure 4 to an oscilloscope and or a data acquisition system Connect the OUT connector of the filter LPBF 01GX to an oscilloscope and a data acquisition system If required 3 Connect the MONITOR OUTPUTS to an oscilloscope to watch the OUTPUTS of the single time constants and the linear component Note In this configuration signals from the TRC 01M are linked to the LPBF 01GX using the EPMS 07 bus 4 2 CE Adjusting the compensation circuit Set the amplifier t
10. o VC mode Apply a test pulse to the system which does not activate ion channels Watch OUT of the filter LPBF 01GX both additional switches TRC TEC in upper position You will see the ohmic response of the cell which is compensated by the LINEAR potentiometer Watch the beginning of the voltage step of the SUMMING OUTPUT of the TRC 01M and CURRENT OUTPUT from the amplifier at an expanded time base Shape the signal at the SUMMING OUTPUT using the three time constant settings so that 1t 1s as identical as possible with the CURRENT OUTPUT signal from the amplifier Ideally this leads to a perfect compensation of the transient Important The values of the time constants generated by the TRC 01M are NOT identical with the real values of the cell membrane version 1 0 page 9 5 Technical Data Module for EPMS 07 System COMMAND IN Low Pass Frequency Range Amplitude Impedance GAIN for T1 T2 and T3 Time constants T1 low pass Time constants T1 high pass Time constants T2 low pass Time constants T2 high pass Time constants T3 low pass Time constants T3 high pass Output Impedances EPMS 07 SYSTEM Power Requirements 115 230 V AC 60 50 Hz fuse 0 8 0 4 A slow 45 60 W depending on the modules plugged 1n Dimensions 300 Hz 3 3 ms to 7 kHz 140 us TRC 01M User Manual 0 to 100 1 e 0 V to 10 V 100 kQ x1 to x50 with trim pot GAIN DEFAULT range x1 range x10 range x100 range xl
11. plifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 0 page 3 TRC 01M User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rackmount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items ar
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