Pierce G2 Fast Blotter Quick Start Guide
Contents
1. 5SIOTLE Quick Start Guide IMPORTANT Review and implement guidelines for proper set up before installation Gels simultaneously transferred must have the same formulation o Using the appropriate power cord supplied with the blotter connect the blotter control unit to an outlet A Switch on the Power Button located on the control unit s rear panel E For each gel use four sheets of 0 83mm thick Western blotting filter paper and one sheet of nitrocellulose or PVDF membrane cut to the same size a Equiliorate filter paper and membrane in Thermo Scientific Pierce 1 Step Transfer Buffer for at least 5 minutes Use sufficient buffer to cover filter paper and membrane SOmL per mini sized 7cm x 8 4cm sandwich and 100mL per midi sized 8cm x 13 5cm sandwich Note PVDF membrane must be wetted with methanol or ethanol before equilibration in Pierce 1 Step Transfer Buffer A After electrophoresis remove gel s from cassette s and briefly place into tray containing deionized water or transfer buffer This will ensure even wetting facilitate proper gel placement and improve gel contact with the membrane 6 Assemble and center sandwich es on bottom of cassette anode to ensure even pressure 10mm spaces between sandwiches Use a blot roller to remove any trapped air bubbles Cathode 2 sheets of pre wet filter paper combined thickness not to exceed 1 8mm Gel Membrane 2 sheets of pre wet filter p2. G Do not overfill the cassette with liquid Excess liquid can overflow into the base unit and possibly cause electric shock Follow the appropriate instructions for reagent amounts and empty any remaining liquid in the cassette upon run completion A h WARNING Ihe blotter base unit can contain dangerous electricity and is not designed to opened by the user NOTE A grounded circuit capable of delivering the appropriate current and voltage is required for installation Electrical requirements can be located on the rear panel of the blotter base The blotter electrical system will adjust to the proper voltage for the respective country Connect the blotter power cord to the rear left side panel of the device and plug into a grounded power outlet Blotter Electrical Parameter Rating Supply Voltage VAC 100 240 Frequency Hz 50 60 Maximum Power Rating VV 168 Fuse Power Center TBAL 250V 3A ENVIRONMENTALLY FRIENDLY DISPOSAL According to EU directive 2002 96 EC on electric and electronic equipment and its implementation into national law all electric equioments must be separately collected and environmentally friendly recycled Alternative disposal If the owner of the electric equipments does not return it to the manufacturer he is responsible for proper disposal at a designated collec tion point that prepares the device for recycling according to national recycling laws and regulations This does not include accessories and tools wi
3. aper combined thickness not to exceed 1 8mm Anode ya Lock top of the cassette cathode into place and slide cassette into the control unit 8 Select Pre Programmed Methods in the Main Menu Main Menu Shut Down 9 Select the number of gels and gel size mini or midi for transfer a 1 mini gel b 2 mini gels or 1 midi gel c 3 mini gels d 4 mini gels or 2 midi gels Pre Programmed Methods Select gel number and size 10 Select the appropriate program to run a Low MW lt 25kDa Mixed Range MW 25 150kDa High MW gt 150kDa Std Semi Dry 1 5mm thick gels or if unknown 2200 Note For fast blotting programs a b c and e use Pierce 1 Stepo Transfer Buffer Transfer time may be increased to 12 minutes for extremely high molecular weight proteins Do not use the Std Semi Dry transfer program d with Pierce 1 Step Transfer Buffer Choose Program 1 mini gel i Transfer Ready Low MW 12 Pierce 1 Step Transfer Buffer is a highly concentrated salt solution Thoroughly wash the anode and cathode after each use and rinse the unassembled cassette under hot water while removing any Sticky salt residue with a gloved hand Rinse with deionized water and stand parts in a rack to dry For more thorough cleaning im merse the cassette top cathode and bottom anode in hot water and use a gloved hand or clean sponge to remove salt residue Rinse with deionized water and stand parts i
4. n a rack to dry NOTE Failure to keep cassette top and bottom clean can result in moving parts sticking and lead to poor transfer efficiency f After running at high current the anode and cathode plates can become hot Use caution when separating the gels and Stacks from the plates Related Thermo Scientific Products 84731 84742 84783 84784 88600 88018 88518 Pierce 1 Stepo Transfer Buffer 1L Pierce 1 Step Transfer Buffer 200mL Western Blotting Filter Paper 7cm x 8 4cm 0 88mm thick 100 mini sized sheets Western Blotting Filter Paper 8cm x 13 5cm 0 83mm thick 100 midi sized sheets Western Blotting Filter Paper 8cm x 10 5cm 0 88mm thick 100 mini sized sheets Nitrocellulose Membrane 0 45um 30cm x 3 5m roll PVDF Membrane 0 45um 26 5cm x 3 75m roll WARNING After running at high current the anode and cathode plates can become hot Use caution when separating the gels and stacks from the plates When continuously processing multipole samples at 5A for a maximum of 2 hours allow the cassette to cool for 30 minutes or use multiple cassettes to avoid excessive cassette heating WARNING Blotter use outside of the workflows described in this manual may put the operator at risk of dangerous expo sure to electrical shock Do not use this instrument for any purposes or in any configurations not described in this manual Disconnect all power to the base unit before maintenance by a qualified technician WARNIN
5. thout electric or electronic components For more details refer to the full blotter user manual available at www thermoscientific pierce A Find us on E Facebook thermoscientific com pierce 2013 Thermo Fisher Scientific Inc All rights reserved Facebook is a registered trademark of Facebook Inc All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Life Science Research Africa Belgium Europe Middle East 32 53 85 71 84 France 0 800 50 82 15 Germany 0228 9125650 Netherlands 0 6 50 31 880 Switzerland 0800 56 31 40 UK 0800 252 185 2162534 05 13 Printed in China Email perbio euromarketing thermofisher com USA 815 968 0747 or 800 874 3723 thermoscientific com pierce Customer Assistance For other regions visit E mail Pierce CS thermofisher com thermoscientific com piercedistributors thermoscientiic com pierce hermo SCIENTIFIC Part of Thermo Fisher Scientific
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