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User`s Guide for OPTIC 2

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2. 1 Is the internal fuse blown 2 Is the power lead to the injector damaged 3 Is there a high resistance contact between the injector power lead and the injector Refer to 11 4 11 3 3 Injector Slow to Heat If the display temperature takes an excessive time to reach the set temperature check the following 1 Is there a high resistance contact between the injector power lead and the injector Refer to 11 4 11 3 4 Injector Slow to Cool If the injector takes an excessive time to cool to the initial temperature set in the method check the following 1 Is the cooling air supply pressure set to 80 PSI 11 4 Injector Power Lead If there is a high resistance contact between the injector power lead and the injector carry out the following procedure 1 Ensure that power to the instrument is switched Off 2 Remove the power lead and inspect the connectors Any pitting or discoloration can be removed by careful use of very fine emery cloth 3 Clean the connectors and reconnect them securely to the injector Page 11 2 Fault Diagnosis Optic 2 Issue 1 12 Maintenance This section provides information on routine and preventive maintenance and servicing for the Optic 2 12 1 Routine Maintenance No special recommendations need to be applied regarding routine maintenance other than that which should be standard good chromatographic practice as follows 1 The septum should be changed regularly 2 The line
3. 00 10 Min Sec Page 6 8 Method Menu Optic 2 Issue 1 Note Setting a Transfer Time shorter than the Splitless Time will increase the time taken for the Initial Run Pressure to be attained 6 5 11 Initial Run Pressure The Initial Run Pressure option defines the pressure that the carrier gas is set to after the transfer time has elapsed this represents the inlet pressure set to use at the start of the chromatographic operation The range is between 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Initial Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 5 12 Final Run Pressure The Final Run Pressure option defines the carrier gas pressure at the end of the method run The range available is between 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Final Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0
4. installed injector 7 Replace the cooling air pipe and clamp it into place with the cooling block Caution Ensure that the cooling air pipe is clamped securely and that it does not contact the central body of the injector 8 Push the white plastic cooling air supply pipe onto the free end of the cooling air pipe 9 Connect the injector thermocouple to the rear panel of the Optic 2 10 Connect the control box and the host GC to the mains power supply 11 Ensure that the end of the sleeved split line is not in contact with anything 12 Fit the plated safety cover and screw it into position 3 1 2 Gas Control Installation Note This section is not applicable to the installation of an Optic 2 200 or Optic 2 300 Optic 2 is compatible with the carrier gas controls supplied with many modern capillary gas chromatographs However the full functionality of the injector might not be available in some cases The installation of Optic 2 with the existing gas controls is a simple case of substituting Optic 2 in place of the standard injector This is achieved by either cutting the pipes which join the standard injector to the gas controls or by replacing the original gas control components for the Optic 2 gas lines If the latter option is taken it is necessary to ensure that the appropriate gas fittings are available The Optic 2 interface kit includes fittings for connection to 1 16 pipes 3 1 2 1 Split Line Page 3 2 Installation
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6. 2 4 8 1 Serial Port Connector 2 4 3 2 Auto sampler Connector 2 4 8 8 Chromatograph Connector 2 4 3 4 Auxiliary Connector 24 4 Pneumatics 2 4 5 Needle Valves and Vents 2 5 System Overview 2 7 2 6 Remote Control 2 7 Method Menus 2 7 1 Split Method Menu 2 7 2 Splitless Method Menu 2 7 3 Large Volume Method Menu 2 7 4 Desorption Method Menu 2 7 5 Expert Method Menu 2 8 Optic 2 200 2 9 Optic 2 300 2 10 Technical Specification 3 Installation 3 1 Installation 3 1 1 Mounting the Injector 3 1 1 1 Location 3 1 1 2 Installation 3 1 2 Gas Control Installation 3 1 2 1 Split Line 3 1 2 2 Carrier Gas Line 3 1 2 3 Septum Purge Line 3 3 3 1 2 4 Cooling Air Line 3 1 3 Logic Connections 3 1 4 Liner and Column Installation 3 1 4 1 Liner Installation 3 1 4 2 Column Installation Contents Issue 1 Page iii Optic 2 4 Configure Menu 4 1 Access the Configure Menu 4 1 1 Password Name 4 1 2 Password Control 4 2 4 1 2 1 Change Password 4 2 Maximum Temperature 4 GC Ready Input 5 GC Ready Output 6 Contrast Control 7 Pressure Units Reset Parameters 4 3 4 2 Exit the Configure Menu 5 Standby Menu 5 1 Access the Standby Menu 5 1 5 1 1 Standby Time 5 1 2 Standby Temperature 5 1 3 Standby Split State 5 1 4 Standby Carrier Pressure 5 1 5 Standby Auxiliary Pressure 5 3 5 1 6 Access the Software Version Number 5 2 Exit the Standby Menu 6 Method Menu 6 1 Method Rules 6 2 Method Statements 6 3 Access the Method Menu 6 4 Split
7. 3 01 Time 4 01 Time 5 01 Time 6 01 Time 7 01 Time 8 Default Parameters Optic 2 200 and 2 300 Standby Menu Appendix C 2 0 1 0 00 M S 35 C 0 30 M S 0 C S 36 C 0 20 M S 0 00 M S 0 1 0 00 M S 35 C 0 C S 36 C 0 20 M S Open 01 KKK 0 00 M S 35 C 0 30 M S End of Sequence 20 C 0 00 M S Open Open 0 00 M S Open 0 00 M S Open Closed 0 00 M S Open 0 00 M S Closed 0 00 M S Open 0 00 M S Closed 0 00 M S Open 0 00 M S Closed 0 00 M S Open 0 00 M S Issue 1 Default Parameters Optic 2 Standby Time Standby Temperature Standby Split Valve Standby C Pressure Standby A Pressure Sequence Menu Method 1 Execute Method 1 Method 2 Execute Method 2 Method 3 Execute Method 3 Method 4 Execute Method 4 Method 5 Execute Method 5 Configure Menu Password Name Password Control Maximum Temperature GC Reagy Input GC Ready Output Contrast Pressure Units Clear Parameters Split Method Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Transfer Pressure Transfer Time Initial Pressure Final Pressure Splitless Method Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Default Parameters Issue 1 Off 30 C Splitless 0 01 Bar 0 01 Bar End of Sequence 1 Times End of Sequence 1 Times End of Sequence 1 Times End of Sequence 1 Times En
8. 6 7 6 Final Temperature 6 17 6 7 7 End Time 6 17 6 7 8 Split Mode 6 17 6 7 9 Sample Sweep Pressure 6 17 6 7 10 Sample Sweep Time 6 18 6 7 11 Desorption Pressure 6 18 6 7 12 Desorption Time 6 18 6 7 13 Transfer Pressure 6 19 6 7 14 Transfer Time 6 19 6 7 15 Initial Run Pressure 6 19 6 7 16 Final Run Pressure 6 19 6 7 17 Exit the Desorption Method Menu 6 20 6 8 Expert Menu 6 21 6 8 1 Access the Expert Method Menu 6 21 6 8 2 Method Name 6 21 6 8 3 Equilibration Time 6 22 6 8 4 Initial Temperature 6 22 6 8 5 Initial Time 6 22 6 8 6 Ramp Sub Menu 6 23 Contents Page v Optic 2 Issue 1 6 8 6 1 Ramp Rate 1 to 4 6 23 6 8 6 ISO Temperature 1 to 4 6 23 6 8 6 3 ISO Time 1 to 4 6 23 6 8 6 4 Exit the Ramp Sub Menu 6 24 Page 6 8 7 Split Valve Sub Menu 6 24 6 8 7 1 Initial State 6 24 6 8 7 2 State 1 6 24 6 8 7 3 Time 1 6 24 6 8 7 4 State 2 6 25 6 8 7 5 Time 2 6 25 6 8 7 6 Exit the Split Valve Sub Menu 6 25 6 8 8 Auxiliary Control Sub Menu 6 25 6 8 8 1 Initial State 6 26 6 8 8 2 Time 1 to 8 6 26 6 8 8 3 Exit the Auxiliary Control Sub Menu 6 26 6 8 9 Carrier Gas Pressure Control Sub Menu 6 26 6 8 9 1 Start Pressure 6 27 6 8 9 2 Time 6 27 6 8 9 3 End Pressure 6 27 6 8 9 4 Exit the Carrier Gas Pressure Control Sub Menu 6 27 6 8 10 Auxiliary Gas Pressure Control Sub Menu 6 27 6 8 10 1 Start Pressure 6 28 6 8 10 2 Time 6 28 6 8 10 3 End Pressure 6 28 6 8 10 4 Exit the Auxiliary Gas Pressure Control Sub Menu 6 28 6
9. D type connector The function and pin connections are compatible with an IBM PC serial port e DSR Autosampler Connector A 15 way female D type connector Du JN ul i ei 6 OV Run Input Logic Chromatograph Connector A 25 way male D type connector 8 Core White 2 Core Red 2 Core Black 9 Logi 8 Core Violet Auxiliary Connector Rear Panel Pin Connections Appendix E 1 Optic 2 Issue 1 A 25 way female D type connector Pin 1 Lx24V to Splitless Solenoid 8 Relay2contacts Appendix E 2 Rear Panel Connections Optic 2 Issue 1 SPLIT MODE METHOD NAME i Equilibration Time Ramp Rate Final Temperature Transfer Pressure Transfer Time Initial Run Pressure Final Run Pressure OE Ramp Rate 1 1 1 1 Final Temperature Transfer Pressure Transfer Time Initial Run Pressure Applicable to Optic 2 200 and 2 300 Method Sheets Appendix F 1 Optic 2 Issue 1 SPLITLESS MODE METHOD NAME gt C Oooo EM Transfer Pressure Initial Run Pressure Final Run Pressure AA Applicable to Optic 2 200 and 2 300 Method Sheets Appendix F 2 Optic 2 Issue 1 LARGE VOLUME MODE METHOD NAME P Vent Time Ramp Rate Final Temperature End Time Split Open Purge Pressure AA Transfer Pressure Transfer Time Initial Run Pressure I Final Run Pressure AN Solvent Thr
10. Menu 6 4 1 Access the Split Method Menu 6 4 2 Method Name 6 4 3 Equilibration Time 6 4 4 Initial Temperature 6 4 5 Ramp Rate 6 4 6 Final Temperature 6 4 7 End Time 6 4 8 Transfer Pressure 6 4 6 4 9 Transfer Time 6 4 10 Initial Run Pressure 6 4 41 Final Run Pressure 6 4 12 Exit the Split Method Menu 6 5 Splitless Menu 6 5 1 Access the Splitless Method Menu 6 5 2 Method Name 6 5 3 Equilibration Time 6 5 4 Initial Temperature 6 5 5 Ramp Rate Page iv Issue 1 Page 4 1 4 1 Contents Optic 2 Issue 1 6 5 6 Final Temperature 6 7 6 5 7 End Time 6 8 6 5 8 Splitless Time 6 8 6 5 9 Transfer Pressure 6 8 Page 6 5 10 Transfer Time 6 9 6 5 11 Initial Run Pressure 6 9 6 5 12 Final Run Pressure 6 9 6 5 13 Exit the Splitless Method Menu 6 9 6 6 Large Volume Menu 6 10 6 6 1 Access the Large Volume Method Menu 6 10 6 6 2 Method Name 6 10 6 6 3 Equilibration Time 6 11 6 6 4 Initial Temperature 6 11 6 65 Vent Time 6 11 6 6 6 Ramp Rate 6 12 6 6 7 Final Temperature 6 12 6 6 8 End Time 6 12 6 6 9 Split Open Time 6 12 6 6 10 Purge Pressure 6 13 6 6 11 Transfer Pressure 6 13 6 6 12 Transfer Time 6 13 6 6 13 Initial Run Pressure 6 14 6 6 14 Final Run Pressure 6 14 6 6 15 Solvent Monitor Threshold 6 14 6 6 16 Exit the Large Volume Method Menu 6 14 6 7 Desorption Menu 6 15 6 7 1 Access the Desorption Method Menu 6 15 6 7 2 Method Name 6 15 6 7 3 Equilibration Time 6 16 6 7 4 Initial Temperature 6 16 6 7 5 Ramp Rate 6 16
11. Vents 2 5 System Overview Page 2 6 Introduction Optic 2 Issue 1 Operation of the Optic 2 is controlled by a menu structure When power is applied to the Optic 2 a system initialisation sequence is performed During this time the LCD display will read Initialising On completion of the initialisation sequence the Top Level Menu is displayed The Top Level Menu contains the following options Standby Menu Method Menu Sequence Menu Copy Menu Delete Menu Configure Menu Use the Left or Right arrow key to select a menu option from the Top Level Menu To gain access to the required menu press the Menu Enter key 2 6 Remote Control A user can configure the Optic 2 to be controlled by a Personal Computer PC To enable this facility 1 Switch power on to the Optic 2 with the Function key held down The Control Remote window is displayed To change back to local control switch the Optic 2 off and then start up as normal 2 7 Method Menus Optic 2 offers many different ways to introduce all kinds of samples into the GC To combine flexibility with a user friendly interface five different method menus are available Split Splitless Large Volume and Desorption methods are designed to give simplified method entry and editing for the most commonly used sample introduction modes In contrast the Expert method menu gives the analyst flexibility to design injection techniques to suit special requirements Up to 20 different methods ca
12. an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 7 15 Initial Run Pressure The Initial Run Pressure option defines the pressure that the carrier gas is set to after the transfer time has elapsed this represents the inlet pressure set to use at the start of the chromatographic operation The range is between 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Initial Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 1 0 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 7 16 Final Run Pressure The Final Run Pressure option defines the carrier gas pressure at the end of the method run The range is between 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Final Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 1 0 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 7 17 Exit the De
13. available To change the Ramp Rate option 1 Use the Up or Down arrow key to move to the required setting Steps are in increments of 1 C s 6 8 6 2 ISO Temperature 1 to 4 The ISO Temperature option defines the temperature of the next iso thermal step in the temperature sequence The range available is between 50 C and 600 C with the default setting at 20 C To change the ISO Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow keys changes the current temperature in steps of 5 C 6 8 6 3 ISO Time 1 to 4 The ISO Time option defines the time that the previous iso thermal temperature is maintained The range available is between 0 00 Min Sec and 100 00 Min Sec with the default setting at 0 00 Min Sec To change the ISO Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 8 6 4 Exit the Ramp Sub Menu To exit the Ramp Sub Menu and return to the Expert Menu 1 Press the Menu Exit key Method Menu Page 6 23 Optic 2 Issue 1 6 8 7 Split Valve Sub Menu The Split Valve Sub Menu allows the user to set individual parameters for two states of the valve not i
14. black nylon coated nut which joins the injector top assembly to the injector body and lift the top assembly to one side Care should be taken not to lose the flat PTFE insulating washer 5 Lift out the existing liner with the O ring in place 6 Insert the new liner and O ring 7 Replace the top assembly and tighten the black nylon coated nut to finger tight Care should be taken to ensure that the flat PTFE insulating washer is fitted correctly Page 3 4 Installation Optic 2 Issue 1 8 Replace the plated safety cover and screw it into position 9 Switch power on to the Optic 2 Caution When installation is complete all connections should be leak tested with an electronic leak detector such as the Ai Leakseaker 196 or a 50 50 solution of iso propanol and water Under no circumstances should a soap solution or similar be used 3 1 4 2 Column Installation 1 the Fit a ferrule of an appropriate internal diameter for the column to be used to nut at the base of the injector 2 If standard Pyrex quartz or thermal desorption liners are being used insert the tip of the column to a depth of 21mm 3 If fritted liners are being used insert the column until the tip touches the underside of the frit and then withdraw the column approximately 2mm 4 On column liners are fitted with the restriction towards the top of the injector and the column introduced into the injector until the column tip engages in the botto
15. defines the time that must elapse since the run output signal was set before the state of the relay is inverted The range available is between 0 00 Min Sec and 120 00 Min Sec with the default setting at 0 00 Min Sec To change the Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 01 00 Min Sec 6 8 8 3 Exit the Auxiliary Control Sub Menu To exit the Auxiliary Control Sub Menu and return to the Expert Menu 1 Press the Menu Exit key Note The following options apply only to the Optic 2 200 and Optic 2 300 6 8 9 Carrier Gas Pressure Control Sub Menu The Carrier Gas Pressure Control Sub Menu allows the user to set individual parameters for up to four events Each event contains the same three parameters Start Pressure Time and End Pressure To enter the Carrier Gas Pressure Control Sub Menu 1 Press the Up or Down arrow key to select the required event 2 Press the Menu Enter key 6 8 9 1 Start Pressure The Start Pressure option defines the level of carrier gas pressure at the start of the event The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Start Pressure option 1 Use the Up or Down arrow key to
16. eliminated 6 6 16 Exit the Large Volume Method Menu To exit the Large Volume Method Menu and return to the Method Menu 1 Press the Menu Exit key Page 6 14 Method Menu Optic 2 Issue 1 6 7 Desorption Menu The Desorption Method Menu is used to set the following functions and parameters Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Split Mode Sample Sweep Pressure Optic 2 200 Sample Sweep Time Optic 2 200 Desorption Pressure Optic 2 200 Desorption Time Optic 2 200 Transfer Pressure Optic 2 200 Transfer Time Optic 2 200 Initial Run Pressure Optic 2 200 Final Run Pressure Optic 2 200 A graphical representation of these parameters is given in Appendix A 6 7 1 Access the Desorption Method Menu To access the Desorption Method Menu 1 Ensure that the display shows Injection Mode and Desorption 2 Use the Left or Right arrow key to select the required parameter 6 7 2 Method Name The Method Name window allows the user to store a customised desorption method under a name of the user s choice To enter a Method Name 1 Press the Function key The flashing cursor is placed on the first position 2 Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first character in the name Method Menu Page 6 15 Optic 2 Issue 1 3 Use the Right arrow key to move the flashing cursor to the next position and repeat ste
17. in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 8 7 4 State 2 The State 2 option defines the state of the split valve at a predefined time The options are Closed Open Optic 2 or Splitless amp Open Vent Optic 2 200 and Optic 2 300 with the default setting at Closed Optic 2 or Splitless Optic 2 200 and Optic 2 300 Page 6 24 Method Menu Optic 2 Issue 1 To change the Split Valve State 2 option 1 Use the Up or Down arrow key to move to the required state 6 8 7 5 Time 2 The Time 2 option defines the time that the split valve assumes a predefined state The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Time 2 option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 8 7 6 Exit the Split Valve Sub Menu To exit the Split Valve Sub Menu and return to the Expert Menu 1 Press the Menu Exit key 6 8 8 Auxiliary Control Sub Menu The Auxiliary Control Sub Menu allows the user to set individual parameters for up to five auxiliary control relays which may be used for the control of external devices Each relay can be individually named and programm
18. move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 Page 6 26 Method Menu Optic 2 Issue 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 8 9 2 Time The Time option defines the time that a linear pressure ramp is maintained from the start pressure to the end pressure The range available is between 0 00 Min Sec and 120 00 Min Sec with the default setting at 0 00 Min Sec To change the Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 01 00 Min Sec 6 8 9 3 End Pressure The End Pressure option defines the level of carrier gas pressure at the end of the event The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the End Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 8 9 4 Exit the Carrier Gas Pressure Control Sub Menu To exit the Carrier Gas Pressure Con
19. of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec Note The following options apply only to the Optic 2 200 and Optic 2 300 6 5 9 Transfer Pressure The Transfer Pressure option defines the pressure in the injector at the start of the method run This enables the user to set the pressure which is applied to the sample to transfer it from the injector onto the column higher pressures give a faster transfer The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Transfer Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 5 10 Transfer Time The Transfer Time option defines the time that the transfer pressure is maintained after the start of the method run The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 20 Min Sec To change the Transfer Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of
20. removal of an injector block entails the removal of the injector heater and temperature sensor Some GCs will treat the absence of the sensor as a fault In such cases a resistor has to be fitted in place of the sensor refer to the installation kit for details and the GC s injector control must be switched off at all times For correct operation the Optic 2 injector base plate must be electrically grounded via the GC The completed installation should be checked by a competent person before use 3 1 1 2 Installation 1 Determine the location for the Optic 2 and if necessary remove or relocate the existing injector Installation Page 3 1 Optic 2 Issue 1 2 Locate the injector cooling block and undo the retaining screw and nut 3 Screw the injector body to the mounting plate provided in the installation kit and offer up the assembly to its intended location 4 Uncoil the split line insulated with fibre glass sleeving and form the pipe to follow the route intended Do the same for the carrier gas inlet line the septum purge line and the injector power lead Note With the pipes pointing towards the rear of the GC the pipe on the left is the septum purge line 5 Screw the Optic 2 to its mounting plate and screw the mounting plate in place on the GC s oven top 6 Inside the column oven pack mineral wool insulation tightly around the injector in order to ensure that hot air from the oven cannot leak out around the newly
21. should only be used if it is intended to operate Optic 2 as a hot isothermal injector for split or splitless injection 1 To use the septum purge facility join the Optic 2 septum purge line to the existing septum purge control within the host GC using a 1 16 Swagelok coupling In this case the purge control should be shut off when not required 2 If itis decided not to use the septum purge the tube can be blanked off at the end using a 1 16 Swagelok coupling and a blanking cap 3 1 2 4 Cooling Air Line Caution When replacing or adjusting the cooling pipe ensure that the unit is switched off Before switching on ensure that no part of the cooling pipe is in contact with either the body of the injector or the power conductor which connects the power cable to the top of the injector For optimum cooling efficiency Optic 2 requires a supply of compressed air at 80psi A lower pressure supply can be used but the speed of cool down will suffer accordingly Installation Page 3 3 Optic 2 Issue 1 While analytical grade air is not needed if air is supplied from a compressor or air line the air supply should be filtered to be free of moisture oil and particulates 1 Connect the air supply directly to the solenoid valve mounted on the rear panel of the control unit 2 Push the white plastic pipe which emerges from the top of the solenoid valve onto the 1 16 stainless steel stub pipe Note The cooling arrangement works o
22. the auto sampler Please contact your local Optic 2 distributor if you have any questions concerning compatibility 2 4 Control Unit 2 4 1 Keypad The Optic 2 has a multi functional keypad mounted on the front panel of the unit The eight keys allow the user to interface with the user friendly software to configure and perform the various functions of the Optic 2 A diagram of the keypad is shown in Figure 2 1 Introduction Page 2 1 Optic 2 Issue 1 INVECTION MODE SPLIT RUN E END Figure 2 1 Display and Keypad 2 4 2 Display Information is presented to the user via a 2 line 20 digit display mounted above the keypad A typical display example is shown in Figure 2 1 2 4 3 Rear Connector Panel The Rear Connector Panel provides connections for the interface with the host GC and the main power supplies A diagram of the panel is shown in Figure 2 2 The six couplings located at the top of the panel provide inlet and outlet connections for Carrier Gas and Auxiliary Gas controls These are optional and are used only on the Optic 2 200 and Optic 2 300 instruments The Carrier In coupling is connected to the carrier gas supply The Carrier Out Septum Purge and Split couplings are connected to the corresponding pipes on the injector The Aux In and Aux Out couplings are used on the Optic 2 300 for connection to the second channel of gas pressure controls The Thermometer connections are used for th
23. to move to the required position and repeat step 2 5 When all characters are correct press the Function key to store the name 6 4 3 Equilibration Time The Equilibration Time allows the user to provide for improved temperature and pressure stability prior to Optic 2 entering the Ready state The Equilibration Time option defines the time between the temperature and pressure stabilising and the Optic 2 entering the Ready state The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Equilibration Time option 1 Use the Up or Down arrow key to move to the reguired time Steps are in increments of 00 30 Min Sec 6 4 4 Initial Temperature The Initial Temperature option defines the injector temperature at the start of the method run The range available is between 50 C and 600 C with the default setting at 35 C To change the Initial Temperature option 1 Use the Up or Down arrow key to move to the reguired temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 5 C 6 4 5 Ramp Rate The Ramp Rate option defines the rate of increase of injector temperature The range available is between 0 C s and 16 C s with the default setting at 0 C s To change the Ramp Rate option Method Menu Page 6 3 Optic 2 Issue 1 1 Use
24. 10 bar 1 0 PSI 10kPa 6 5 13 Exit the Splitless Method Menu To exit the Splitless Method Menu and return to the Method Menu 1 Press the Menu Exit key Method Menu Page 6 9 Optic 2 Issue 1 6 6 Large Volume Menu The Large Volume Method Menu is used to set the following functions and parameters Method Name Equilibration Time Initial Temperature Vent Time Ramp Rate Final Temperature End Time Split Open Time Purge Pressure Optic 2 200 Transfer Pressure Optic 2 200 Transfer Time Optic 2 200 Initial Run Pressure Optic 2 200 Final Run Pressure Optic 2 200 Solvent Monitor Threshold Optic 2 200 A graphical representation of these parameters is given in Appendix A Note There is a fixed 10 second delay after the Vent Time before the start of the ramp This improves the transfer of volatile components onto the column 6 6 1 Access the Large Volume Method Menu To access the Large Volume Method Menu 1 Ensure that the display shows Injection Mode and Large Volume 2 Use the Left or Right arrow key to select the required parameter 6 6 2 Method Name The Method Name window allows the user to store a customised large volume method under a name of the user s choice To enter a Method Name Page 6 10 Method Menu Optic 2 Issue 1 1 Press the Function key The flashing cursor is placed on the first position 2 Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first
25. 2 200 Initial Run Pressure Optic 2 200 Final Run Pressure Optic 2 200 A graphical representation of these parameters is given in Appendix A 6 5 1 Access the Splitless Method Menu To access the Splitless Method Menu 1 Ensure that the display shows Injection Mode and Splitless 2 Use the Left or Right arrow key to select the required parameter 6 5 2 Method Name The Method Name window allows the user to store a customised splitless method under a name of the user s choice To enter a Method Name 1 Press the Function key The flashing cursor is placed on the first position 2 Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first character in the name 3 Use the Right arrow key to move the flashing cursor to the next position and repeat step 2 4 Repeat step 3 for the remaining characters in the name If a mistake is made use the Left or Right arrow key to move to the required position and repeat step 2 5 When all characters are correct press the Function key to store the name 6 5 3 Equilibration Time The Equilibration Time allows the user to provide for improved temperature and pressure stability prior to Optic 2 entering the Ready state Page 6 6 Method Menu Optic 2 Issue 1 The Equilibration Time option defines the time between the temperature and pressure stabilising and the Optic 2 entering the Ready state The range available is between 0 00 Min Sec
26. 4 146191 126155 7 3298 146178 126025 126018 126054 7 2900 7 2757 7 3803 7 1336 146492 7 3846 7 3847 146141 146142 146143 146144 146189 146189 146168 146184 Issue 1 Pack Size 50 50 10 10 25g cl cl Ll l la c Page 13 1 Optic 2 Electronic Auxiliary Gas Control 146185 VOC Sparger Kit 136500 13 6 Installation Kits includes Interface Cables HP5890 146121 Varian 3300 3400 146122 Carlo Erba 8000 146123 HP6890 146125 Ai 92 93 94 95 146126 General Purpose 146127 Page 13 2 Issue 1 k l l la c Spares and Accessories Optic 2 Issue 1 The Kr rra ited om RUN Miun can be zm d by u 1 pisa p Fw uni Enid ny han Opcion Ha Fey ie by moni dan len command ra Opie ard iun anm uidi be preh o remi dh haa nid tun n iha hasi GC T mrm hin hag wra nun ole arm Tiu VN Fo ERE NN Fon Ope 220 Oe ZIM Memo m us able Tal Pan kO cei Paddy ope Fun in dg Era nip Picos ab a Tal Ford 11 05 PED rm Figure A 1 Split Method Profile Method Profiles Appendix A 1 Optic 2 Fu main KOR R 9 m r ra PL TI nie 2 50 oe 2200 dota 2d Spira Vane OR GE Po LA A A hia Pic a ARTE SIT T RA mD VOR r9 T R Memo maun abi 1569 Fan 1 k 0 5 dn Pocas Hab ls Tal Fori 11 05 PSG Method Profiles Issue 1 Tha Erri Ta o mm rcm ion RUN Liun cam bm ll y Dion niha uni Er Lay hn Ch 613 1n 1 Fey 4966 Er vau gi o an djem un cerand C hs CO
27. 9 Exit the Method Menu 6 28 7 Sequence Menu 7 1 7 1 Access the Sequence Menu 7 1 7 2 Set Up a Sequence 7 1 7 8 Exit the Sequence Menu 7 1 8 Copy Menu 8 1 8 1 Access the Copy Menu 8 1 8 2 Copy a Method 8 1 8 3 Exit the Copy Menu 8 2 9 Delete Menu 9 1 9 1 Access the Delete Menu 9 1 9 2 Delete a Method 9 1 9 3 Exit the Delete Menu 9 1 10 Performing a Method 10 1 10 1 Run a Method 10 1 10 1 1 Loading a Method 10 2 10 2 Displays 10 2 10 2 1 Optic 2 10 2 10 2 2 Optic 2 200 10 2 10 2 3 Optic 2 300 10 2 10 3 End of Method 10 3 Page vi Contents Optic 2 11 11 1 k k k k k k Daza to 11 2 1 11 2 2 11 3 1 11 3 2 11 3 3 11 3 4 11 4 12 12 1 12 2 13 13 1 13 2 13 3 13 4 13 5 13 6 Fault Diagnosis Warnings Ramp Rate Carrier Gas Faults Thermocouple Fault Temperature Fault Troubleshooting No Power Injector Does Not Heat Injector Slow to Heat Injector Slow to Cool Injector Power Lead Maintenance Routine Maintenance Servicing Spares and Accessories Injector Liners Consumables Spares Interface Cables Accessories and Upgrades 13 1 Installation Kits includes Interface Cables List of Illustrations Figure 2 1 Display and Keypad Figure 2 2 Rear Connector Panel Figure 2 3 Pneumatics Figure 2 4 Needle Valves and Vents Figure 10 1 State Transition Diagram Appendices NnMUOOU gt Method Profiles Menu Flow Charts Default Parameters Injector Diagram Rear Panel Pin Connecti
28. Gas Off Carrier Gas Pressure set to 0 1bar Auxiliary Gas Pressure set to 0 1bar In order to allow safe handling of injector for thermal desorption Carrier Gas Off Function Revert to Standby conditions Method Carrier Gas Off Up arrow Revert to Initial Temperature Initialising and Pressure of Method Note When operating in the Large Volume mode it is important to first press the Run key and then inject the sample 10 2 Displays The displays presented during a run are different between Optic 2 Optic 2 200 and Optic 2 300 10 2 1 Optic 2 The user can choose between the following displays Temperature Method or Valve State To select a display press the Function key until the display required is shown 10 2 2 Optic 2 200 The user can choose between the following displays Temperature Method Valve State or Carrier Gas Pressure To select a display press the Function key until the display required is shown 10 2 3 Optic 2 300 The user can choose between the following displays Temperature Method Valve State Carrier Gas Pressure or Auxiliary Gas Pressure To select a display press the Function key until the display required is shown 10 3 End of Method Page 10 2 Performing a Method Optic 2 Issue 1 When the method has been completed the display shows Initialising The display remains until the initial temperature and pressure conditions are established at which point the display chang
29. Optic 2 User Manual ATAS International B V Veldhoven The Netherlands Disclaimer ATAS International B V makes no representations or warranties with respect to the contents or use of this manual and specifically disclaims any express or implied warranties of merchantability or fitness for any particular purpose Further ATAS International B V reserves the right to revise this manual and to make changes to its content at any time without obligation to notify any person or entity of such revisions or changes Copyright No part of this manual may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopying and recording for any purpose other than the purchaser s personal use without the written consent of the copyright holder O ATAS International B V April 1996 All rights reserved ATAS International B V De Run 4441 5503 LS Veldhoven The Netherlands Telephone 31 0 40 254 95 31 Facsimile 31 0 40 254 97 79 Optic 2 6 is a registered trademark of ATAS International B V Document Number 146008 Revision History Issue Date Description 1 04 06 1996 First Edition 2 Second Edition Optic 2 Contents EC Declaration of Conformity About This Manual Contents Warnings and Cautions 1 Warnings 2 Cautions 2 Introduction 2 1 Improved Performance 2 2 Improved Flexibility 2 3 Compatibility 24 Control Unit 2 4 1 Keypad 2 4 2 Display 2 4 3 Rear Connector Panel
30. Optic 2 Issue 1 The split line is sleeved with braided insulation When Optic 2 is switched on the split line is electrically live 2V AC While this does not represent a risk of electric shock it is important for correct operation of the injector that the split line is electrically isolated from the rest of the chromatograph A 1 16 nylon Swagelok coupling is provided for this purpose 1 Determine the required length of the split line Using a sharp blade cut the insulation around the pipe and remove the unwanted insulation 2 Cut the pipe to the required length using a tube cutter 3 Insert the pipe into one end of the nylon coupling as far as possible and tighten the fitting The braided insulation should butt up against the fitting leaving no uninsulated pipe exposed 4 Connect the free end of the nylon coupling to the 1 16 split line from the host chromatograph 3 1 2 2 Carrier Gas Line The carrier gas inlet pipe and septum purge outlet pipe are located side by side on the injector top assembly Note lt is important to correctly identify these two pipes With the top assembly in place on the injector and the pipes pointing towards the rear of the GC the carrier gas inlet pipe is the pipe on the right 1 Join the carrier gas line from the host GC to the Optic 2 injector using a 1 16 Swagelok coupling 3 1 2 3 Septum Purge Line In most operating modes the use of a septum purge offers no advantage This facility
31. Pins 1 and 2 Relay contacts Run Input Type External contact closure Function When the connections are shorted the Optic goes into the Run state Pins 5 System reference voltage ground 6 Logic input pulled up internally to 5V via resistor network 2 4 3 3 Chromatograph Connector A 25 way male D type connector Run Output Type A relay contact closure for one second when the Run state is entered Function Run signal which is output at the end of the initial isothermal period Pins 21 and 22 Relay contacts Integrator Run Output Type A relay contact closure for one second when the Run state is entered Function Run signal which is output at the end of the initial isothermal period Pins 23 and 24 Relay contacts GC Ready Output This signal is output to the GC to indicate the ready state of the Optic Type Relay contacts asserted when the instrument is in the Ready state Normally Open Normally Closed Disabled Open or Disabled Closed selected through configuration menu Function Signal to other parts of the system that the Optic and devices upstream in the Ready chain are ready for an injection Pins 19 and 20 Relay contacts GC Ready Input This signal is received from the GC Page 2 4 Introduction Optic 2 Issue 1 Type External contact closure or contact open on Ready or disabled This will be selectable through the configuration menu The default setting is disabled Function When a Ready si
32. RE ar iun shpuri bu nnm tih Em arei iun o ine a Faro Fale i hia empa al am 1 Fit nna FF m 1 1 Ti mi PENINE ma Arrin YA Fi y dip Fun in nip End al p Figure A 2 Splitless Method Profile Appendix A 2 Optic 2 Issue 1 Ta Ere Tra cx im mm ion A Liun cm bm le by Dien Iha Plu et Lav han Opica n ihe Fey adaa by mai Ones run carm ha Opie are cun ahn uid mc nod vesh ibm and iun ihe hem CO 1 1 1 F ral mmm dua TR mom a MIR 1 1 1 1 10 emi Fa Pa 1 Day dle k Ertan Urra 1 l ES iS E nim me a 1 1 has Trad 1 LI LI U a 1 E E Tixr m Ya A P TIS Fio RO F ma Pi Pura Pina ipn l pr ZM Ope 2200 A 1 i hini Fou m 7 nimi Damm 1 t gt O ka ra k h i pla pl K x him erm i i at wani Toi Yaku ani s 1 1 t s Tar m zd oa m Haba rd Fan VE di mwa Fady nip Fun io Fun n kx End Jam Pocos sisht al Fori 11 05 FE Term Figure A 3 Large Volume Method Profile Method Profiles Appendix A 3 Optic 2 Issue 1 Tha Ere Tra n eee mm RE Liun on bs ll by 1 cm ihm Rudi kae stan Opica n lhe Rumi aana by tog D mi MEL Opie end tun she ui be 1 archon oi dh lha m rcd iun 9 ha ka CC Fra area aii Ta rr R hini rpm si am w 1 Tian aan Fie F mal Pic Pio ope 2200 ope 200 Faro m 915 Hable Tal Fan E OS depa Feni Fun 1 lalo Fumie a de Tal Poni I D FO lab le rm Figure A 4 Desor
33. Ready Input should be disabled When the Optic 2 is to be the master device ie the Optic 2 Run Output starts the GC then the GC Ready Output should be disabled and the GC Ready Input should be enabled in either Open When Ready or Closed When Ready as appropriate To change the GC Ready Output option 1 Use the Up or Down arrow key to select the parameter required either Closed When Ready Open When Ready Disabled Closed or Disabled Open 4 1 6 Contrast Control The Contrast Control option allows the user to alter the contrast of the LCD display To change the Contrast Control option 1 Use the Up key to increase or the Down key to decrease the contrast of the LCD display 4 1 7 Pressure Units The Pressure Units option allows the user to change the units of measurement for the gas pressure throughout the system To change the Pressure Units option 1 Use the Up or Down key to select the required unit of measurement The options are bar PSI or kPa When this setting is changed all of the pressure values are automatically converted by Optic 2 4 1 8 Reset Parameters Selecting this option resets all of the instrument settings to the factory default values This option can be used to return the instrument to a known configuration Note A list of the factory default values is given in Appendix C 4 2 Exit the Configure Menu To exit the Configure Menu and return to the Top Level Menu 1 Press the M
34. The options are between Closed amp Open Optic 2 and Splitless Split lt gt Vent Optic 2 200 and Optic 2 300 with the default position set to Closed Optic 2 and Splitless Optic 2 200 and Optic 2 300 To change the Split Mode option 1 Use the Up or Down arrow key to move to the required state Note The valve is held in this state for the duration of the run It is usually an advantage to operate with the valve in the split state and the split flow approximately the same as the column flow Note The following options apply only to the Optic 2 200 and Optic 2 300 6 7 9 Sample Sweep Pressure The Sample Sweep Pressure option defines the carrier gas pressure during the time that the sample is being swept with carrier gas prior to desorption The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Sample Sweep Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 7 10 Sample Sweep Time Method Menu Page 6 17 Optic 2 Issue 1 The Sample Sweep Time option defines the time that the sample is swept with carrier gas prior to desorption in order to eliminate air from the in
35. and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Equilibration Time option 1 Use the Up or Down arrow key to move to the required time Steps are in increments of 00 30 Min Sec 6 5 4 Initial Temperature The Initial Temperature option defines the injector temperature at the start of the method run The range available is between 50 C and 600 C with the default setting at 35 C To change the Initial Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 5 C 6 5 5 Ramp Rate The Ramp Rate option defines the rate of increase of injector temperature The range available is between 0 C s and 16 C s with the default setting at 0 C s To change the Ramp Rate option 1 Use the Up or Down arrow key to move to the required setting Steps are in increments of 1 C s 6 5 6 Final Temperature The Final Temperature option defines the injector temperature at the end of the method run The range is between 50 C and 600 C with the default setting at 36 eom To change the Final Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the curren
36. ature Maximum Temperature set in Configure Menu Final Temperature Maximum Temperature Final Temperature Initial Temperature Final Run Pressure Initial Run Pressure Initial Run Pressure Transfer Pressure Split Open Time Vent Close Time Ramp Temperature Ramp 1 Initial Temperature Ramp Temperature Ramp N 3 Ramp Temperature Ramp N 1 ISO Temperature Ramp N Maximum Temperature A ramp rate must be set in order for the ramp to be executed during a method run Split Valve Time 2 3 Split Valve Time 1 Auxiliary Time N Auxiliary Time N 1 Carrier Gas End Pressure Carrier Gas Start Pressure Auxiliary Gas End Pressure Auxiliary Gas Start Pressure If any of the method rules are violated a warning message is displayed for two seconds The user will be unable to exit the menu until the violation is corrected 6 2 Method Statements All parameters referring to Times are referenced from 0 Run output set Method Menu Page 6 1 Optic 2 Issue 1 6 3 Access the Method Menu To access the Method Menu 1 Switch power on and wait for the system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Method Menu 3 Press the Menu Enter button 4 If the system is protected by Password Control enter the password as described in Section 4 1 1 and press the Function Key If the password is correct access is granted to the first option in the Method Men
37. character in the name 3 Use the Right arrow key to move the flashing cursor to the next position and repeat step 2 4 Repeat step 3 for the remaining characters in the name If a mistake is made use the Left or Right arrow key to move to the required position and repeat step 2 5 When all characters are correct press the Function key to store the name 6 6 3 Equilibration Time The Equilibration Time allows the user to provide for improved temperature and pressure stability prior to Optic 2 entering the Ready state The Equilibration Time option defines the time between the temperature and pressure stabilising and the Optic 2 entering the Ready state The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Equilibration Time option 1 Use the Up or Down arrow key to move to the required time Steps are in increments of 00 30 Min Sec 6 6 4 Initial Temperature The Initial Temperature option defines the injector temperature at the start of the method run The range available is between 50 C and 600 C with the default setting at 35 C To change the Initial Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 5 C 6 6 5 Vent Time The Vent Time o
38. characters are correct press the Function key to store the name 2 Press the Menu Enter button The selected method is copied to the destination method and a confirmation message is displayed for two seconds Copy Menu Page 8 1 Optic Il Issue 1 8 3 Exit the Copy Menu To exit the Copy Menu and return to the Top Level Menu 1 Press the Menu Exit key Page 8 2 Copy Menu Optic 2 Issue 1 9 Delete Menu The Delete Menu allows the user to delete a method When a method is deleted all the current parameters in the method are reset to their default values 9 1 Access the Delete Menu To access the Delete Menu 1 Switch power on and wait for the system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Delete Menu 3 Use the Up or Down key to select the method to be deleted 4 Press the Menu Enter button 5 If the system is protected by Password Control enter the password as described in Section 4 1 1 If the password is correct access is gained to the first option in the Standby Menu If the password is incorrect a prompt is displayed to re enter the password 6 Press the Function key 7 The Delete Confirmation window is displayed 9 2 Delete a Method To delete the selected method 1 Use the Up or Down arrow key to select Yes 2 Press the Menu Enter button The selected method is deleted and a confirmation message is displayed for two seconds 9 3 Exit the Delet
39. d of Sequence 1 Times kiki No 600 C Disabled Closed when ready 20 Bar No 1 0 00 M S 35 C 0 C S 36 C 0 20 M S 0 10 Bar 0 20 M S 0 02 Bar 0 02 Bar 0 1 KKK 0 00 M S 35 C 0 C S 36 C 0 20 M S Appendix C 3 Optic 2 Splitless Time Transfer Pressure Transfer Time Initial Pressure Final Pressure Large Volume Method Method Name Equilibration Time Initial Temperature Vent Time Ramp Rate Final Temperature End Time Split Open Time Purge Pressure Transfer Pressure Transfer Time Initial Pressure Final Pressure Solvent Threshold Desorption Method Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Split Mode Sweep Pressure Sweep Time Desorption Pressure Desorption Time Transfer Pressure Transfer Time Initial Pressure Final Pressure Expert Method Method Name Equilibration Time Initial Temperature Initial Time Ramp Sub Menu Ramp Rate ISO Temperature Appendix C 4 Issue 1 1 00 M S 0 10 Bar 0 20 M S 0 02 Bar 0 02 Bar 0 1 kkk 0 00 M S 35 C 0 30 M S 0 C S 36 C 0 20 M S 0 00 M S 0 02 Bar 0 10 Bar 0 20 M S 0 02 Bar 0 02 Bar 0 840 fee 0 00 M S 35 C 0 C S 36 C 0 20 M S Splitless 0 02 Bar 0 15 M S 0 00 Bar 1 00 M S 0 10 Bar 0 20 M S 0 02 Bar 0 02 Bar 01 KKK 0 00 M S 35 C 0 30 M S End of Sequence 20 C Default Parameters Optic 2 ISO Time Split Valve Sub Menu Ini
40. di ba sre o remi dh haar tun n iha hasi GC Turm slum hag erp ole arm Tiu VN Fo ERE R Peau pir 2 200 Oe ZIM LI Turo mud Fish Tal Poni H D 5 dapma Fey a p Fun ip ag Pie Habe Tal Forni 11 05 FTE rm Figure A 1 Split Method Profile 1 E End nip Method Profiles Appendix A 1 Optic 2 Issue 1 Tm Erei Pr o ca ion RUN Liun can bm nxi by ocn niha uni Er Lay hn Ch 613 1n Fey deo Er mel o an djem un coon Tha CORE ard iun so uii be MATE ond dh Em and iun ol haha CO 1 F ral Perra dum Turm bum 1 1 LI 1 U Faro Faa Erud balon rra y L 7 bal Tarmpmalum LI 1 1 k Fica nra rim gar AR Fa perans E RRR pr ZM Oe 2 Wata EAT AA SEM 35 TT hini Pcie Z AE AZET ARE GTA GT STE E A Ca T9 aja y 1 U zdim ie vam N Told 4 1 LI wani Told Waku yani LI Tart main Habe 129 Fani OS dee Faad y oo Fun lin ni Erd do Pim sHabh Tel Fori 11 05 Feo r ra Figure A 2 Splitless Method Profile Method Profiles Appendix A 2 Optic 2 Turm E Erud balon r ra Tar m 91 abi Gal Fan k di Pocas sisht al Fori 11 05 FIT Method Profiles Issue 1 Ta Km Cre mimm mn ion FM A cmn em om und by DI RY la Pun n ke yan Opica n ih Remi ada by mg o anaie U HEB mo 9 Ma Opie are iun ann uid be preti m ri v sh ia 89 tin 9 ha ra CC ID farmi Pa mg Fala Day pa c x z 1 U Pda dae Ta TT a mm 1 by dra Mai ATE T S 1 hioi rna 3
41. e Menu To exit the Delete Menu and return to the Top Level Menu 1 Press the Menu Exit key Delete Menu Page 9 1 Optic 2 Issue 1 10 Performing a Method Figure 10 1 illustrates the stages that Optic 2 goes through as it prepares to run a method haw Turri si uum hix Com Femen hix Auca ica y F n h Faby T hix ouem ET OPEH INJEGTOR Thermal Dasarptian Figure 10 1 State Transition Diagram 10 1 Run a Method To run a method carry out the following sequence le Switch power on and wait for the system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Method Menu 3 Use the Up or Down arrow key to select the method to be run 4 Press the Function key 5 When the Ready message is displayed press the Run End key 10 1 1 Loading the Method Performing a Method Page 10 1 Optic 2 Issue 1 In Programme Mode the temperature and pressure are maintained at the levels set in the Standby Menu When the Function key is pressed after a method has been selected the instrument initialises to the temperature and pressure parameters of the selected method During loading stages of the method the action of certain keys changes the condition and display of the instrument as shown below Stage Key Condition Display Initialising Function Revert to Standby conditions Method Ready Function Revert to Standby conditions Method Ready Down arrow Temperature set to 35 C Carrier
42. e and particulate matter from reaching the column Consequentially cold splitless injection is better suited to the analysis of dirty samples 2 7 3 Large Volume Method Menu In the past gas chromatographers have been used to injecting samples of no more than 2ml into the GC Since split splitless injectors generally have an internal capacity of approximately 2ml and 1ml of solvent gives rise to approximately 1ml of vapour the total amount of sample that can be injected into a split or splitless injector is around 2m l When an injection is made into a temperature programmable injector the evaporation of the solvent is a controllable process and therefore restrictions on the volume of solvent that can be injected are much less severe Optic 2 s large volume method is designed to implement the Rapid large volume technique This enables injections up to 100ml to be made routinely and with a minimum of optimisation The technique is compatible with most auto samplers and is very robust making it suitable for routine applications Other large volume sampling techniques such as speed programmed injection may also be accommodated In large volume injection the sample is introduced with the injector at near ambient temperature The solvent is allowed to evaporate into the carrier stream and pass out through the split vent At an appropriate point the split vent is closed and the injector heated The sample is then transferred to the colu
43. e injector temperature sensor The Air In port of the Cooling Gas Valve is used for connection to the cooling air supply for the injector The To Injector port should be connected to the cooling pipe of the injector using the plastic tubing supplied Page 2 2 Introduction Optic 2 Issue 1 FREF SEPTUM EU OLI PURSE KAJI STA s SE 5 ALJA IH Gb D 3x THERNONCTER SERIAL E A 1H INJECTOR AUTD SAMPLER Eas Vre T Gas V CHRONAT DGA A al It i B PELNAN TEZO n ESO 23D 5080 Hr lnd Mains Inet 2604 Switch and Fuse ej r y ADDPESS LABEL L A r a SEF NO LABEL r 1 Figure 2 2 Rear Connector Panel Four connectors provide connections for Serial Auto sampler Chromatograph and Auxiliary interfaces The pin allocations for the connectors are shown in the following sub sections Introduction Page 2 3 Optic 2 Issue 1 2 4 3 1 Serial Port Connector A 9 way female D type connector The function and pin connections are compatible with an IBM PC serial port Pin2 TxD Pin3 RxD Pin DTR Pin B OV Pin6 DSR 2 4 3 2 Auto sampler Connector A 15 way female D type connector mainly used when a non standard Auto sampler is fitted to the GC Optic Ready Output Type Normally open relay contacts closed when the instrument is in the Ready state Function Signal to other parts of the system that the Optic and devices upstream in the Ready chain are ready for an injection
44. e number of ul to be used in the large volume injection using clean solvent Set the vent time to the solvent elimination time Set the detector sensitivity to that used to obtain the reference chromatogram Inject the selected volume of dilute standard do not exceed 90 VMAX Compare the chromatogram obtained with the reference chromatogram 6 FINE TUNING If the chromatogram from the large volume injection looks like the reference chromatogram no further optimisation is required If volatile components are lost reduce the vent time in steps of 3 5 7 HINTS Do not be tempted to take short cuts Set the vent flow to 200cm min Set the split flow to 50cm min Set the initial temperature in the range 30 40 C below the solvent boiling point Set the final temperature to 50 C above the elution temperature of the latest running peak Set the ramp rate to 4 C min VMAX is usually 80 125ul Solvent elimination time is usually 30 50 seconds Polar solvents have larger elimination times Quick Reference Guide Optic 2 When using GC MS the rise in source pressure can be used to determine the solvent elimination time Quick Reference Guide Optic 2 Issue 1 1 Warnings and Cautions This section explains the warnings and cautions that should be observed when installing or operating the Optic 2 Safety information is covered at relevant points throughout the manual Please read this manual in its entirety before install
45. ec To change the Desorption Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 7 13 Transfer Pressure The Transfer Pressure option defines the pressure in the injector at the start of the method run This enables the user to set the pressure which is applied to the sample to transfer it from the injector onto the column higher pressures give a faster transfer The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Transfer Pressure option Page 6 18 Method Menu Optic 2 Issue 1 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 7 14 Transfer Time The Transfer Time option defines the time that the transfer pressure is maintained after the start of the method run The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 20 Min Sec To change the Transfer Time option ds Use the Up or Down arrow key to move to the reguired time Single presses of
46. ed to act on up to eight events These events are available on the Aux port located on the rear connector panel for more information on the Aux port refer to Section 2 To enter the Auxiliary Control Sub Menu 1 Press the Up or Down arrow key to select the required auxiliary relay The user can now enter a name for the selected relay To enter a name carry out the following procedure a Press the Function key The flashing cursor is placed on the first position b Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first character in the name C Use the Right arrow key to move the flashing cursor to the next position and repeat step b d Repeat step c for the remaining characters in the name If a mistake is made use the Left or Right arrow key to move to the required position and repeat step b e When all characters are correct press the Function key to store the name 2 Press the Menu Enter key Method Menu Page 6 25 Optic 2 Issue 1 3 Press the Left or Right arrow keys to select the required parameters 6 8 8 1 Initial State The Initial State option defines the state of the auxiliary relay when the instrument is initialised on loading the method The options are Closed lt gt Open with the default setting at Closed To change the Initial State option 1 Use the Up or Down arrow key to move to the required state 6 8 8 2 Time 1 to 8 The Time option
47. enu Exit key Configure Menu Page 4 3 Optic 2 Issue 1 5 Standby Menu The Standby Menu is used to set the following functions and parameters Standby Time Standby Temperature Standby Split State Standby Carrier Pressure Optic 2 200 and Optic 2 300 Standby Auxiliary Pressure Optic 2 300 Access the Software Version Number 5 1 Access the Standby Menu To access the Standby Menu 1 Switch power on and wait for the system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Standby Menu 3 Press the Menu Enter button 4 If the system is protected by Password Control enter the password as described in Section 4 1 1 and press the Function Key If the password is correct access is granted to the first option in the Method Menu If the password is incorrect a prompt is displayed to re enter the password 5 Use the Left or Right arrow key to select the option reguired 5 1 1 Standby Time The Standby Time option defines the length of time that Optic 2 remains in the Ready state after which it will default to the standby settings The range available is between Off never goes to standby and 20 00 Min Sec with the default setting at Off To change the Standby Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 1 second Continuous pressure on an arrow key changes the current setting in ste
48. es to Ready The temperature and gas settings are maintained at their initial levels for the duration of the Standby period defined in the Standby Menu When this period has elapsed the instrument reverts to the standby conditions and the display shows Method If the Standby parameter in the Configure Menu is set to Off the initial conditions of the method are maintained until the next press of the Run End key Performing a Method Page 10 3 Optic 2 Issue 1 11 Fault Diagnosis This section details the warning and fault conditions on the Optic 2 and the states within the software which they are valid 11 1 Warnings Warnings are only detected after a method has been selected and loaded Warnings are displayed until canceled by pressing the Menu Enter key and are not re enabled until the instrument is switched off and back on again 11 1 1 Ramp Rate The Ramp Rate warning is enabled and valid when a ramp temperature output is being performed and the ramp temperature is more than 50 C above or below the set point 75 C above or below a set point above 450 C On detection of this condition the display reads Warning Ramp Rate 11 1 2 Carrier Gas The Carrier Gas warning is enabled and valid when the carrier gas pressure is at zero On detection of this condition the display reads Warning Carrier Gas 11 2 Faults Fault conditions on the Optic 2 are non recoverable To clear a fault condition the inst
49. eshold AAA Applicable to Optic 2 200 and 2 300 Method Sheets Appendix F 3 Optic 2 Issue 1 DESORPTION MODE METHOD NAME oporo oo o O Ramp Rate Final Temperature End Time Split Mode Sample Sweep Pressure I Sample Sweep Time Desorp Pressure M Desorp Time Transfer Pressure ss Transfer Time Initial Run Pressure E II T Final Run Pressure Applicable to Optic 2 200 and 2 300 EXPERT MODE Method Sheets Appendix F 4 Optic 2 Issue 1 METHOD NAME Equilibration Time Initial Temperature A ES Oe Initial State Initial State MWI AAA Initial State Method Sheets Appendix F 5 Optic 2 Issue 1 pa AAA or k jj AUX 4 Initial State L L L L L L L L L L CARRIERGAST Stari Pressure End Pressure iJ Method Sheets Appendix F 6 Optic 2 Issue 1 CARRIER GAS 2 Start Pressure Time Time CARRIER GAS 3 Time Time A CARRIER GAS 4 Start Pressure Time Time Applicable to Optic 2 200 and 2 300 AUX GAS 1 Start Pressure Time 0 0 0 0 Time AA AUX GAS 2 Time Time AAA AAN AUX GAS 3 Time Time po AUX GAS 4 Time Time Applicable to Optic 2 300 Method Sheets Appendix F 7 Optic 2 Issue 1 The Kr rra ca om RUM Run can be led by 1 pisa p Ha uni Enid un Opcion Ha Pv ie by moi ola clara iun command ra Opec iun anm u
50. gnal is received no action is taken until the Optic is ready and the equilibration time has passed When all these conditions are satisfied the Optic enters the Ready state and the Ready output becomes active Pins 3 System reference voltage ground 4 Logic input pulled up internally to 45V via resistor network Run Input Type External contact closure Function When the connections are shorted the Optic goes into the Run state Pins 9 System reference voltage ground 10 Logic input pulled up internally to 45V via resistor network 2 4 3 4 Auxiliary Connector A 25 way female D type connector Splitless Valve Type Drive to solenoid valve Function Controlled by software Provides the drive to switch the splitless solenoid as required Pins 1 Connected to output 24V supply see note at end 2 Connected to relay or open collector drive rated to 30V 250mA max Auxiliary Output Functions 1 to 5 Type Normally open relays rated to 30V 250mA under software control for external devices Function Control of additional solenoid valves or other external devices Pins 4and5 Connected to relay 1 contacts 7and8 Connected to relay 2 contacts 10 and 11 Connected to relay 3 contacts 14 and 15 Connected to relay 4 contacts 17 and 18 Connected to relay 5 contacts 20 and 21 Connected to output 24V supply 22 and 23 Connected to output 5V supply 24 and 25 Connected to OV return 5V supply This is a voltage of 5V nominal rela
51. gree and substantial amounts of solvent vapour may emerge from the split vent and purge ports It is highly recommended that this effluent be ducted to a fume hood or that active carbon traps are fitted to the outlets 1 2 Cautions The Optic 2 gas controls are not designed for operation with explosive corrosive or toxic gases It is important that a good connection is made between the injector power cable the flat copper lead and the injector A bad connection can result in poor operation and the connection becoming excessively hot Ensure that the cooling air pipe is clamped securely in place and does not contact the central body of the injector or the injector power cable Warnings and Cautions Page 1 1 Optic 2 Issue 1 When leak testing connections ensure that a suitable electronic leak detector such as the Ai Leakseaker 196 or a 50 50 solution of iso propanol and water is used Under no circumstances should a soap solution or similar be used The injector control box must not be switched on with the injector disconnected Serious damage to the instrument may be caused Page 1 2 Warnings and Cautions Optic 2 Issue 1 2 Introduction Until now Gas Chromatographs GCs have been fitted with injectors which are simple in design and very limited in the options that they offer For many years sample injection has been the weakest part of chromatographic system imposing severe limitations in it s ability to deliver high
52. h the installation kit Should you intend to install Optic 2 onto an instrument for which a specific installation kit is unavailable please follow these instructions with particular care 3 1 Installation The installation of Optic 2 to an existing GC can be considered as four separate tasks Installation of the injector into the chromatograph oven Connection of the injector to the gas controls Electrical connections Installation of an appropriate liner and column WARNING Itis important that both the Optic 2 and the host GC are disconnected from the mains supply until the installation is complete 3 1 1 Mounting the Injector To achieve the best results from Optic 2 the injector should be installed directly into the oven insulation rather than into an injector block Fitting Optic 2 in this way ensures that it cools rapidly to a start temperature 3 1 1 1 Location If Optic 2 is to be used for manual injection only it can be fitted anywhere on the top of the oven away from the injector and detector blocks The most convenient location is one that aligns with an existing hole in the column oven s inner skin If Optic 2 is to be used with an auto sampler extra care is needed in deciding upon the location In many cases the removal or relocation of an existing injector block will be required Ensure that the new injector is aligned in all three axis such that its septum cap is co incident with that of the standard injector Note The
53. ing the pressure in the injector during solvent elimination can be set independently In desorption applications desorption of the sample can take place under static or dynamic flow conditions In addition Optic 2 200 offers the following features A sensor in the split line enables the progress of solvent elimination to be monitored and if desired closure of the split valve can be automated A separate control enables the vent flow during solvent elimination to be set independent of the split flow 2 9 Optic 2 300 Optic 2 300 offers all of the advantages of the Optic 2 200 and includes a second independent channel of pressure programming which is accessible via the Expert method menu 2 10 Technical Specification Operating Ranges Air Cooling 35 C to 600 C at an ambient temperature of 25 C and a GC oven temperature of 35 C Liquid CO Cooling 50 C to 600 C at an ambient temperature of 25 C and a GC oven temperature of 35 C Ambient Temperature Range 10 C to 40 C Mains Power For use with 220 240V 10 50 Hz or 110 120V 10 60 Hz Power Consumption 250VA Gas Supplies Carrier amp Auxiliary Helium Nitrogen or Argon at a pressure between 30 and 100psi Optic Cooling Moisture and oil free air at a pressure between 60 and 80psi Page 2 10 Introduction Optic 2 Issue 1 3 Installation This section should be read carefully in conjunction with any specific instructions supplied wit
54. ing or operating the instrument Should any point remain unclear contact your supplier for assistance before proceeding Optic 2 is designed for installation and use in a laboratory environment by suitably trained personnel 1 1 Warnings Optic 2 is intended for use in laboratories which are equipped to enable analytical chemistry to be performed without risk In particular provision must be made for the storage of solvents in flame proof cabinets and personnel must wear protective clothing and eye protection at all times Both the Optic 2 and the host GC must be disconnected from the mains power supply until installation of the equipment is complete There are potentially hazardous voltages present inside the Optic 2 control unit Switch off the control unit and disconnect the mains power supply before removing the control unit cover The injector unit is supplied with a plated safety cover This prevents contact with potentially hazardous electrical supplies and hot surfaces This cover MUST be in place at all times when the Optic 2 is switched on The operation of the injector requires that it heats rapidly to high temperatures Parts of the injector may remain hot for some time after the unit is switched off Care must be taken when working in the area of the injector It is especially important to ensure that the injector is cool below 60 C and switched off before changing injector liners Most organic solvents are toxic to some de
55. inuous pressure on an arrow keys changes the current setting in steps of 00 10 Min Sec Page 6 12 Method Menu Optic 2 Issue 1 Note The following options apply only to the Optic 2 200 and Optic 2 300 6 6 10 Purge Pressure The Purge Pressure option defines the pressure during the Initial Time while the solvent is eliminated through the vent line The range is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Purge Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 6 11 Transfer Pressure The Transfer Pressure option defines the pressure in the injector at the start of the method run This enables the user to set the pressure which is applied to the sample to transfer it from the injector onto the column higher pressures give a faster transfer The range is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Transfer Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pres
56. ion 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa When the user exits the Standby Menu the pressure is adjusted to the new standby auxiliary gas pressure setting If the Function key is pressed while Standby Aux Pressure is displayed the window changes to Aux Pressure Now and the actual pressure of the standby auxiliary gas is displayed To return to the Standby Aux Pressure window press the Function key 5 1 6 Access the Software Version Number This option allows the user to view the version number of the software in current use To view the current software version number 1 Ensure that the display reads Standby Menu 2 Press the Function key The display shows the current software version To return to the Standby Menu 1 Press the Function key 5 2 Exit the Standby Menu To exit the Standby Menu and return to the Top Level Menu 1 Press the Menu Exit key Standby Menu Page 5 3 Optic 2 Issue 1 6 Method Menu The Method Menu is used to access the following options Split Menu Splitless Menu Large Volume Menu Desorption Menu Expert Menu 6 1 Method Rules When parameters are set in a method the following rules apply to their values or states Note Initial Temper
57. jector The range is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 15 Min Sec To change the Sample Sweep Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 7 11 Desorption Pressure The Desorption Pressure option defines the carrier gas pressure applied while the sample is being desorbed In many cases it is beneficial to desorbe the sample with desorption pressure set to zero ie under static flow conditions The range is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Desorption Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 7 12 Desorption Time The Desorption Time option defines the time that the desorption pressure is maintained after the injector begins to heat This will usually be about 30 seconds after the injector reaches the maximum temperature The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 1 00 Min S
58. m of the restriction 5 Tighten the nut at the base of the injector until the column is retained within the injector Care must be taken not to tighten the base nut excessively Caution When installation is complete all connections should be leak tested with an electronic leak detector such as the Ai Leakseaker 196 or a 50 50 solution of iso propanol and water Under no circumstances should a soap solution or similar be used Installation Page 3 5 Optic 2 Issue 1 4 Configure Menu The Configure Menu is used to set the following functions and parameters Password Name Password Control Maximum Temperature GC Ready Input GC Ready Output Contrast Control Pressure Units Reset Parameters 4 1 Access the Configure Menu To access the Configure Menu 1 Switch power on and wait for the system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Configure Menu 3 Press the Menu Enter button 4 If the system is protected by Password Control enter the password as described below and press the Function Key If the password is correct access is granted to the first option in the Method Menu If the password is incorrect a prompt is displayed to re enter the password 5 Use the Left or Right arrow key to select the option required 4 1 1 Password Name The Password Name option defines the characters used in the password to gain access to the system To enter a password carry out the foll
59. mn Simple optimisation of the parameters enables components with a very wide range of volatility to be analysed by this technique The advantages to be gained from this method are very significant An improvement in detection limits of up to 100 times that of conventional splitless injection Page 2 8 Introduction Optic 2 Issue 1 Much greater flexibility in design of sample preparation procedures Opportunities to couple on line with sample preparation accessories such as automated solid phase extraction instruments 2 7 4 Desorption Method Menu The Optic 2 can be used for the thermal desorption of both solid samples and adsorption tubes When analysing gas phase samples the samples can be collected off line using an Optic liner packed with a suitable sorbent Alternatively on line analysis is possible with the sample being introduced into the liner while it is still within the injector The desorption method gives a sequence of operations which is designed to enable the best results to be easily obtained Thermal desorption using the Optic 2 is distinguished from conventional thermal desorption by the way the adsorption tube is closely coupled to the column This gives the following advantages The analysis of very active components is possible since the opportunities for adsorptive losses are greatly reduced The analysis of components with a very high boiling point such as polymer additives is possible The very shor
60. n 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Initial Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 4 11 Final Run Pressure The Final Run Pressure option defines the carrier gas pressure at the end of the method run The range available is between 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Final Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 4 12 Exit the Split Method Menu To exit the Split Method Menu and return to the Method Menu 1 Press the Menu Exit key 6 5 Splitless Menu The Splitless Method Menu is used to set the following functions and parameters Method Name Equilibration Time Initial Temperature Method Menu Page 6 5 Optic 2 Issue 1 Ramp Rate Final Temperature End Time Splitless Time Transfer Pressure Optic 2 200 Transfer Time Optic
61. n be set up at any one time Note Appendix F contains blank Method Sheets which can be photocopied for recording the parameters set in a customised method 2 7 1 Split Method Menu Optic 2 can be used as a conventional hot split injector or for cold split injection In virtually all cases better results will be obtained using the cold split approach This Introduction Page 2 7 Optic 2 Issue 1 means starting with the injector cold i e below the boiling point of the solvent and temperature programming the injector to a temperature usually 50 C above the maximum elution temperature of the sample This gains the following advantages Syringe needle discrimination is eliminated thus ensuring that the fraction of the sample which enters the column is truly representative of the sample as a whole The milder temperature regime experienced by the sample reduces thermal degradation especially when lower injector programming rates are used Better detection limits are obtained for late running components Better run to run reproducibility 2 7 2 Splitless Method Menu Optic 2 can be used for conventional hot splitless injection or for the cold equivalent In virtually all cases better results will be obtained using the cold injection technique for identical reasons to those described above The quality of results obtained should be comparable to on column injection an all respects but with the added advantage of preventing involatil
62. n the principle of entraining a large volume of ambient air in a jet of compressed air emerging from the end of the tube In some instances the installation can result in the injector being enclosed e g under an auto sampler plate and may not represent the most effective cooling arrangement In these cases the crimped tube should be replaced with a longer pipe without a restriction which reaches close to the bottom of the body of the injector This will allow a higher flow rate of compressed air to cool the injector 3 1 3 Logic Connections Optic 2 has a full range of external logic functions which permit control to be integrated with the host GC system These functions are accessible via a 25 way D type connector on the rear connection panel of the control box Instructions for the configuration of these functions is given in the installation guide of the host GC 3 1 4 Liner and Column Installation The way in which the column should be installed and the type of injection port liner required depends upon the role that the Optic 2 is to perform 3 1 4 1 Liner Installation A liner is installed in the injector prior to delivery To change the liner the following procedure should be adopted Caution During this operation care should be taken not to stress the septum purge and carrier gas pipe lines 1 Cool the injector to below 60 C 2 Switch off the Optic 2 3 Remove the plated safety cover from the injector 4 Unscrew the
63. nables the user to set the pressure which is applied to the sample to transfer it from the injector onto the column higher pressures give a faster transfer The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Transfer Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 4 9 Transfer Time Page 6 4 Method Menu Optic 2 Issue 1 The Transfer Time option defines the time that the transfer pressure is maintained after the start of the method run The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 20 Min Sec To change the Transfer Time option 1 Use the Up or Down arrow key to move to the reguired time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 4 10 Initial Run Pressure The Initial Run Pressure option defines the pressure that the carrier gas is set to after the transfer time has elapsed this represents the inlet pressure set to use at the start of the operation The range available is betwee
64. ncluding the initial state Each state consists of the same two events Valve State and Valve Time To enter the Split Valve Sub Menu 1 Press the Menu Enter key 2 Press the Left or Right arrow keys to select the required parameters 6 8 7 1 Initial State The Initial State option defines the state of the split valve when the instrument is initialised on loading the method The options are Closed lt gt Open Optic 2 or Splitless lt gt Split Vent Optic 2 200 and Optic 2 300 with the default setting at Closed Optic 2 or Splitless Optic 2 200 and Optic 2 300 To change the Initial State option dh Use the Up or Down arrow key to move to the required state 6 8 7 2 State 1 The State 1 option defines the state of the split valve at a predefined time The options are Closed Open Optic 2 or Splitless Split amp Vent Optic 2 200 and Optic 2 300 with the default setting at Closed Optic 2 or Splitless Optic 2 200 and Optic 2 300 To change the Split Valve State 1 option 1 Use the Up or Down arrow key to move to the required state 6 8 7 3 Time 1 The Time 1 option defines the time that the split valve assumes a predefined state The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Time 1 option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting
65. ne method to another When a method is copied all data is transferred to the new method except for the method name 8 1 Access the Copy Menu To access the Copy Menu 1 Switch power on and wait for the system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Copy Menu 3 Use the Up or Down key to select the method to be copied 4 Press the Menu Enter button 5 If the system is protected by Password Control enter the password as described in Section 4 1 1 If the password is correct access is gained to the first option in the Standby Menu If the password is incorrect a prompt is displayed to re enter the password 6 Press the Function key 8 2 Copy a Method To copy the selected method 1 Use the Up or Down arrow key to move to the destination method The display shows HERNIA where NN defines the destination method number To edit the name of the destination method carry out the following procedure a Press the Function key The flashing cursor is placed on the first position b Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first character in the name C Use the Right arrow key to move the flashing cursor to the next position and repeat step b d Repeat step c for the remaining characters in the name If a mistake is made use the Left or Right arrow key to move to the required position and repeat step b e When all
66. nges the current temperature in steps of 5 C 6 6 8 End Time The End Time option defines the time between the start and end of the GC method run This should be set to the total run time for the GC analysis in order for the cooling of the injector and resetting of pressure parameter to coincide with the GC run end The range is between 0 00 Min Sec and 120 00 Min Sec and Infinite Infinity with the default setting at 0 15 Min Sec To change the End Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 10 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 01 00 Min Sec 6 6 9 Split Open Time The Split Open Time option defines the period of time after the start of the method before the split line is opened When setting this parameter the time taken for the injector to reach its final temperature must be taken into account Typically splitless times will be longer than for a corresponding iso thermal splitless injection by an amount relating to the rise time of the injector temperature The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 00 00 Min Sec To change the Split Open Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Cont
67. nt setting in steps of 0 10 bar 1 0 PSI 10kPa 6 6 14 Final Run Pressure The Final Run Pressure option defines the carrier gas pressure at the end of the method run The range is between 0 00 bar 0 0 PSI OkPa and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Final Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 6 15 Solvent Monitor Threshold The Solvent Monitor Threshold is the value used to automatically determine the solvent elimination time This parameter sets the threshold for a sensor that monitors the presence of solvent in the split line The sensor reading displayed during this phase of the run must rise above the threshold during injection The run starts when the reading falls back below the threshold The range is between 0 and 700 with the default setting at O To change the Solvent Monitor Threshold option 1 Use the Up or Down arrow key to move to the required setting Steps are in increments of 1 Note When using this facility with manual injections Large Volume with Vent Time set to Auto press the Run key then inject the sample The run will automatically start when the solvent has been
68. nt temperature in steps of 5 C 6 8 5 Initial Time The Initial Time option defines the time period that the initial temperature and pressure are maintained This period may be used for solvent elimination The range is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 30 Min Sec To change the Initial Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec 6 8 6 Ramp Sub Menu The Ramp Sub Menu allows the user to set individual parameters for up to four ramps Each ramp sequence contains the same three events Ramp Rate ISO Page 6 22 Method Menu Optic 2 Issue 1 Temperature and ISO Time The ramp sequences should be selected in turn from this level ie Ramp 1 is selected and edited then return to this menu and select Ramp 2 etc To enter the Ramp Sub Menu 1 Press the Up or Down arrow key to select the required ramp sequence 2 Press the Menu Enter key 3 Press the Left or Right arrow keys to select the ramp parameters 6 8 6 1 Ramp Rate 1 to 4 The Ramp Rate option defines the rate of increase of injector temperature The range available is between 0 C s and 16 C s with the default setting at End of Sequence The display shows End of Sequence when there are no further ramps
69. on is made 5 1 4 Standby Carrier Pressure This option is available if you have an Optic 2 200 or Optic 2 300 The Standby Pressure option allows the user to set the level of the standby carrier gas pressure The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Standby Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa When the user exits the Standby Menu the pressure is adjusted to the new standby carrier gas pressure setting If the Function key is pressed while Standby Pressure is displayed the window changes to Pressure Now and the actual pressure of the standby carrier gas is displayed To return to the Standby Pressure window press the Function key 5 1 5 Standby Auxiliary Pressure This option is available if you have an Optic 2 300 Page 5 2 Standby Menu Optic 2 Issue 1 The Standby Auxiliary Pressure option allows the user to set the level of the standby auxiliary gas pressure The range available is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Standby Auxiliary Pressure opt
70. ons Method Sheets Contents NN 12 1 12 1 12 1 13 1 13 1 13 1 13 1 13 1 13 2 A1 A5 B1 B3 C1 C5 D1 E1 E2 F1 F7 Issue 1 Page vii Optic 2 amp Back Ta e abr Mad Cate 2200 and Date 2300 Stand by Aux Preu re kl sred Far Gai To hla ni NAU lov Chart em gt Mara Edi hiy 0 A Bock Ta s A Back Ta COY Mea Delete fena Cony Ta Dekre np kaa Y E kaad Nara E rar Fi ac Dada Tul Mara rdar Kaj A Bak Ta Cardigan Mer Quick Reference Guide Optic 2 Split Mat hei laa eni Danit 2200 and Daie 2 300 Run Run Fas E End E Tr neder E Traneler d Initial ir Final B Time Pressure Time S plit Lass Method a Bark Ta i Mav Manu lt lt Neu Ed ba Run Run Manilar Th rei dd J S ra rm Initial Y Final K Salveni pi F re ure F ees ure Time Tis u ARA AS A PROA Desorption Mat ha Quick Reference Guide Optic 2 A Back Ta Mew Men l Gate 2 200 and une Kar Da o 2 300 gm EE H Naw Ed Kar Miseni a A ME Hara Bl Kay Split Valve Sheri Carrier sr Pressure Gana 4 Aur Cue Pressure Ca niral4 Carrier Gas Pese ure Canina Aux ae Few ure Dux as Sub Menu Ga niai Quick Reference Guide Optic 2 OPTIMISATION ROUTINE FOR RAPID OR AT ONCE LARGE VOLUME INJECTION 1 OBTAIN A REFERENCE CHROMATOGRAM Either from previous work or by using an on column injection of a 1
71. ove to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 01 00 Min Sec 6 8 10 3 End Pressure The End Pressure option defines the level of carrier gas pressure at the end of the event The range is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the End Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 8 10 4 Exit the Auxiliary Gas Pressure Control Sub Menu To exit the Auxiliary Gas Pressure Control Sub Menu and return to the Expert Menu 1 Press the Menu Exit key 6 9 Exit the Method Menu To exit the Method Menu and return to the Top Level Menu 1 Press the Menu Exit key Page 6 28 Method Menu Optic 2 Issue 1 7 Sequence Menu The Sequence Menu allows the user to configure the Optic 2 to run a number of methods in a set sequence The maximum number of methods allowed in any sequence is five Each method within the sequence can be configured to run up to five times 7 1 Access the Sequence Menu To access the Sequence Menu 1 Switch power on and wait for the
72. owing sequence 1 Press the Function key The flashing cursor is placed on the first position 2 Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first character in the password 3 Use the Right arrow key to move the flashing cursor to the next position and repeat step 2 Configure Menu Page 4 1 Optic 2 Issue 1 4 Repeat step 3 for the remaining two positions If a mistake is made use the Left or Right arrow key to move to the required position and repeat step 2 5 When the password is correct press the Function key to store the password 4 1 2 Password Control The Password Control option enables or disables the password protection for the system To enable password protection 1 Use the Up or Down arrow key to change the parameter to Yes To disable password protection 1 Use the Up or Down arrow key to change the parameter to No 4 1 2 1 Change Password If the password has to be changed it may have been lost or forgotten carry out the following sequence 1 Switch power on to the Optic 2 with the Menu Enter key held down 2 Enter the new password using the sequence described in Section 4 1 1 4 1 3 Maximum Temperature The Maximum Temperature option sets the maximum temperature permissible for the injector The range is between 50 C and 600 C with the default setting at 600 C To change the Maximum Temperature 1 Use the Up or Down arrow key to mo
73. p 2 4 Repeat step 3 for the remaining characters in the name If a mistake is made use the Left or Right arrow key to move to the required position and repeat step 2 5 When all characters are correct press the Function key to store the name 6 7 3 Equilibration Time The Equilibration Time allows the user to provide for improved temperature and pressure stability prior to Optic 2 entering the Ready state The Equilibration Time option defines the time between the temperature and pressure stabilising and the Optic 2 entering the Ready state The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Equilibration Time option 1 Use the Up or Down arrow key to move to the reguired time Steps are in increments of 00 30 Min Sec 6 7 4 Initial Temperature The Initial Temperature option defines the injector temperature at the start of the method run The range available is between 50 C and 600 C with the default setting at 35 C To change the Initial Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 5 C 6 7 5 Ramp Rate The Ramp Rate option defines the rate of increase of injector temperature The range available is between 0 C s and 16 C s wi
74. ppendix A 5 Optic 2 Issue 1 amp Back Ta Srardiry Mena Ca o 2200 aid Daie 2300 ER UEM St nd by Aux Preeeu re lari Kay Da ToN abad N aru iiw Charta Pua Z Yd Y EE EE E hara dl Hay aback Ta B A Berk Ta Con Meni Delete MZ Delete ENDE ID MEE Nara E rda Fic Dubia Tul Mara rka Fay A pack Ta Carta r Puk ry Menu Flow Charts Appendix B 1 Optic 2 Issue 1 Danit 2200 and Daie 2 300 Run Aun E End e Ml Initial ji Bal Hi Time Pressure Time S plit Lass Method Large Volume Mat hoad a Bark Ta i Maced Manu lt lt Neu td Kar Run Run Manilar Preu re Pres ure Th resh E de 3 Purge ea LE Initial E Final sane P rex ure Pressure Time Desorption Mat ha Appendix B 2 Menu Flow Charts Optic 2 Issue 1 Expart A Back m Method Man Menn l Cac 2 200 and une kar Cate 2 300 PAISES Mani Cdi faa J Neu Ed ar Nara dl Kay Split Valve Sheri A A A ONES om Carrier Gat Aur Ge Pressure Pressure Cantal 4 Ta ntrald A Carrier s Aur Ga Carrier Gas P rece ure Aur fas Pres ure Sub Menu Tantali Sub Meru Ca nirali Neu End Stiri End Preu re Preu re Preexu e 1 mM E Nea Ed Far Haru Ed kay Menu Flow Charts Appendix B 3 Optic 2 Issue 1 Default Parameters Optic 2 Standby Menu Standby Time Standby Temperature Standby Split Valve Sequence Menu Method 1 Execute Method 1 Me
75. ps of 10 seconds 5 1 2 Standby Temperature The Standby Temperature option defines the temperature of the injector in the standby state The range available is between 50 C and 600 C with the default setting at 30 C To change the Standby Temperature Standby Menu Page 5 1 Optic 2 Issue 1 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow keys changes the current temperature in steps of 5 C When the user exits the Standby Menu the temperature of the injector is adjusted to the new value Ifthe Function key is pressed while Standby Temperature is displayed the window changes to Temperature Now and the actual temperature of the injector is displayed To return to the Standby Temperature window press the Function key Note The Standby Temperature setting must be less than the maximum temperature set in the Configure Menu 5 1 3 Standby Split State The Standby Split State option defines the state of the split valve The options are between Closed lt gt Open Optic 2 or Splitless lt Split Vent Optic 2 200 and Optic 2 300 with the default values of Closed Optic 2 and Splitless Optic 2 200 and Optic 2 300 To change the Standby Split State option 1 Use the Up or Down arrow key to move to the required state The split valve is exercised whenever a new selecti
76. ption Method Profile Method Profiles Appendix A 4 Optic 2 Issue 1 1 1 he rd Carm o iu m od ion PN un cun ina RU d by 1 x pra inm Rud ura hey than Ch GG niha Feah 2 zin G by 1 1 m vec C o an de ra run oro he Opice ard tun shoki be 1 seh ori cl h lha are iun bha ead CC ba wra i N ba mm A 1 1 ho rra 9 am Fei 1 1 a m ramp 2 1 i 1 1 1 in arm 2 Farro Gan 3 ii t S bo Tama Z E l Pem Fal Z 1 t 1 im wra 1 E 1 8 im amp K 1 1 Tar luia a k N Ram Fal 1 b noi Fra 1 hiz aro aa Q U i LI w LI LI Gum Go TT TS C xi BH waman Poni Ou kul m E 3 ba End Asmus 5 w 1 1 Tim hemn 7 d Pu U x LI N 1 Hal Aamun d qg M o R U E LI yi 1 1 g Mala za Thl Pumas m Finxuim U i Pao 1 Tukul Y 1 1 z End Aamun 5 pi E Hal Fiamma 2 H Piece Z LI b LI E i h Hal Ariun L K E LI N LI N LI LI 1 LI 1 LI 1 1 P 1 rimis 1 Tok 1 Hani 1 FLI IFLI LET7 VET 1 Thin xx abra i 1 y wl 1 S y 1 1 N s LI 1 1 N 1 N N k 1 y E U LI U 1 E O K U LI U Akay f 99 Ga llad y 1 1 5 Din mari 1 term 1 U trm 2 1 t 1 U LI Timm a A k 1 Timm db a s i e h Term pe 1 1 Term O mir U E LI ja 18 ba asn r rra E Tar abla Gal Poni 1 L 0 3 denim Famy Fun Fun dus End nio lar lw lan Picos abs 7m Fon TK 05 PSU Figure A 5 Expert Method Profile Method Profiles A
77. ption defines the time period that the initial temperature and pressure are maintained This period is provided for solvent elimination The range is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 30 Min Sec To change the Vent Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec Note Atthe end of the Vent Time the split valve closes and after a 10 second delay the injector starts to ramp and the Run Output signal is given 6 6 6 Ramp Rate Method Menu Page 6 11 Optic 2 Issue 1 The Ramp Rate option defines the rate of increase of injector temperature The range available is between 0 C s and 16 C s with the default setting at 0 C s To change the Ramp Rate option 1 Use the Up or Down arrow key to move to the required setting Steps are in increments of 1 C s 6 6 7 Final Temperature The Final Temperature option defines the injector temperature at the end of the method run The range is between 50 C and 600 C with the default setting at 36 eC To change the Final Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key cha
78. quality results Optic 2 is the world s most advanced injection system and is designed to combine excellent performance with a dramatic improvement in analytical flexibility 2 1 Improved Performance The Optic 2 when correctly set up offers complete absence of sample discrimination Reproducibility is comparable to on column injection but offers excellent tolerance of dirty samples Thermal degradation is less severe than with conventional hot split and splitless injectors and the analyst has much greater control over the way in which the sample is treated 2 2 Improved Flexibility The Optic 2 can be used for hot split hot splitless cold split cold splitless and on column injection It is also able to offer injection modes which have no parallel where traditional injectors are concerned Facilities such as large volume sampling thermal desorption and pyrolysis are standard features Optic 2 offers far greater control over the fate of the sample in the injector than normal systems When the sample is injected the analyst can select the temperature and if the equipment is an Optic 2 200 can also select the injector pressure It is even possible to inject under static gas flow conditions Consequently it is possible to conceive many novel approaches to sample injection 2 3 Compatibility Optic 2 is designed to be compatible with most popular makes and models of GC and GC MS Once installed Optic 2 should be fully compatible with
79. r should be inspected periodically and if found to be contaminated should be re packed or replaced as appropriate 12 2 Servicing The Optic 2 is not designed to be user serviced other than the trouble shooting recommendations contained in Section 11 If a fault occurs that can not be rectified by following the recommendations in Section 11 contact your local agent for advice Maintenance Page 12 1 Optic 2 13 Spares and Accessories 13 1 Injector Liners Standard Pyrex Liner Quartz Liner Double Neck Liner 0 32mm On Column Liner 0 53mm On Column Liner 0 32mm Packed On Column Liner 0 53mm Packed On Column Liner Fritted Pyrex Liner Liner Blanking Cap 13 2 Consumables Septa Liner O Rings 0 32mm Graphite Ferrules 0 53mm Graphite Ferrules Silanized Glass Wool 13 3 Spares Top Boss Insulator Injector Nut Nylon Coupling Standard Septum Cap 1 Clamp Nut Insulator Top Clamp Nut Top Boss Braze Assembly Needle Valve Solenoid Valve 1 Electronic Pressure Regulator 1 Pressure Sensor 1 8 1 16 PTFE Ferrules In Line Particulate Filter Replacement Element for Particulate Filter 13 4 Interface Cables HP5890 Varian 3300 3400 Carlo Erba 8000 HP6890 Ai General Purpose 13 5 Accessories and Upgrades Liquid CO Coolant Trapping Kit Electronic Carrier Gas Control Spares and Accessories 126225 126226 126227 126228 126229 126230 126231 126232 146193 146190 126151 126152 126153 7 281
80. rument must be switched off the fault rectified and the instrument switched back on again 11 2 1 Thermocouple Fault The thermocouple fault occurs when the thermocouple fails the thermocouple cable is disconnected or if the GC is not earthed The condition is flagged up by a hardware interrupt On detection of the fault the display reads Fault Thermocouple The heater output is disabled to put the instrument in a safe condition The instrument must be switched off and the fault rectified 11 2 2 Temperature Fault The temperature fault occurs should the current injector temperature be 50 C or higher above the maximum temperature set in the Configure Menu and rising On detection of the fault the display reads Fault Temperature The heater output is disabled to put the instrument in a safe condition The instrument must be switched off and the fault rectified 11 3 Troubleshooting Fault Diagnosis Page 11 1 Optic 2 Issue 1 Should any difficulty arise with the installation or operation of the Optic 2 check the following symptoms before contacting your supplier 11 3 1 No Power If the instrument fails to power up check the following 1 Is the power lead fully inserted into the power socket 2 Is the mains fuse intact UK only 3 Is the fuse located in the instrument power socket intact 11 3 2 Injector Does Not Heat If the instrument powers up but the injector does not heat up check the following
81. sorption Method Menu To exit the Desorption Method Menu and return to the Method Menu 1 Press the Menu Exit key Method Menu Page 6 19 Optic 2 Issue 1 Page 6 20 Method Menu Optic 2 Issue 1 6 8 Expert Menu The Expert Method Menu is used to set the following functions and parameters Method Name Equilibration Time Initial Temperature Initial Time Ramp Sub Menu Ramp 1 Up to Ramp 4 Ramp Rate ISO Temperature ISO Time KO CO CO GO Split Valve Sub Menu Initial State Up to Aux 5 Split State 1 Split Time 1 Split State 2 Split Time 2 KO CO CO CO oO Initial State Up to 4 Time 1 Time 2 etc up to Time 8 Auxiliary Control Sub Menu KO CO cO Carrier Gas Pressure Sub Menu Optic 2 200 Start Pressure Up to 4 Time End Pressure CO CO Oo Auxiliary Gas Pressure Sub Menu 9 Start Pressure Optic 2 300 9 Time 9 End Pressure A graphical representation of these parameters is given in Appendix A 6 8 1 Access the Expert Method Menu To access the Expert Method Menu 1 Ensure that the display shows Injection Mode and Expert 2 Use the Left or Right arrow key to select the required parameter 6 8 2 Method Name The Method Name window allows the user to store a customised expert method under a name of the user s choice To enter a Method Name 1 Press the Function key The flashing cursor is placed on the first position Method Menu Page 6 21 Optic 2 Issue 1 2 Use the Up and Down arrow ke
82. sure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 6 12 Transfer Time The Transfer Time option defines the time that the transfer pressure is maintained after the start of the method run The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 20 Min Sec To change the Transfer Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 01 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 10 Min Sec Note Setting a Transfer Time shorter than the Split Open Time will increase the time taken for the Initial Run Pressure to be attained 6 6 13 Initial Run Pressure The Initial Run Pressure option defines the pressure that the carrier gas is set to after the transfer time has elapsed this represents the inlet pressure set to use at the start of the chromatographic operation The range is between 0 00 bar 0 0 PSI OkPa Method Menu Page 6 13 Optic 2 Issue 1 and 7 00 bar 101 0 PSI 700kPa with the default setting at 0 02 bar 0 2 PSI 2 kPa To change the Initial Run Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 PSI 1kPa Continuous pressure on an arrow key changes the curre
83. system Initialisation sequence to complete 2 Use the Left or Right arrow key to select the Sequence Menu 3 Press the Menu Enter button 4 If the system is protected by Password Control enter the password as described in Section 4 1 1 If the password is correct access is gained to the first option in the Standby Menu If the password is incorrect a prompt is displayed to re enter the password 5 Press the Function key 6 The first Method window is displayed 7 2 Set Up a Sequence To set up a sequence of methods le Use the Up or Down arrow key to move to the method that is to be first in the sequence The range is from the first method 01 through to the last method 20 and Sequence End The default setting is Sequence End Steps are in increments of 1 2 Use the Left or Right arrow key to move to the Execute Method window 3 Use the Up or Down arrow key to select the number of times the method is to be run The range is from 1 to 5 with the default value setto 1 Steps are in increments of 1 4 Use the Left or Right arrow key to move to the next the Method window 5 Repeat steps 1 to 4 until the required sequence is complete 6 At the Method window select Sequence End 7 3 Exit the Sequence Menu To exit the Sequence Menu and return to the Top Level Menu 1 Press the Menu Exit key Sequence Menu Page 7 1 Optic 2 Issue 1 8 Copy Menu The Copy Menu allows the user to copy o
84. t temperature in steps of 5 C 6 5 7 End Time The End Time option defines the time between the start and end of the GC method run This should be set to the total run time for the GC analysis in order for the cooling of the injector and resetting of pressure parameter to coincide with the GC run end The range is between 0 00 Min Sec and 120 00 Min Sec and Infinite Infinity with the default setting at 0 20 Min Sec To change the End Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 10 Min Sec Method Menu Page 6 7 Optic 2 Issue 1 Continuous pressure on an arrow key changes the current setting in steps of 00 30 Min Sec 6 5 8 Splitless Time The Splitless Time option defines the period of time after the start of the method before the split line is opened When setting this parameter the time taken for the injector to reach its final temperature must be taken into account Typically splitless times will be longer than for a corresponding iso thermal splitless injection by an amount relating to the rise time of the injector temperature The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 01 00 Min Sec To change the Splitless Time option 1 Use the Up or Down arrow key to move to the reguired time Single presses of an arrow key change the current setting in steps
85. t time cycle associated with the desorption enables results to be obtained rapidly 2 7 5 Expert Method Menu The four modes of operation described above are designed to cover the most common ways in which Optic 2 will be used The Expert mode is designed to cater for the many other possibilities In the expert mode four ramp temperature programmes and four segment pressure profiles can be set In addition five external channels of time events can be programmed and the split valve can be programmed to change state up to eight times in any sequence Pressure control of an auxiliary gas supply channel is also possible 2 8 Optic 2 200 Optic 2 200 offers all of the advantages of the Optic 2 with the additional benefit of electronic programming of the carrier gas pressure The gas controls resident within the Optic 2 200 are designed to enable the fullest advantage to be taken of the equipment s analytical capabilities Pressure programming gives the following advantages During the transfer of the sample from the injector to the column an elevated pressure can be set in order to speed the transfer This can give sharper peaks and will also assist in the reduction of thermal degradation Introduction Page 2 9 Optic 2 Issue 1 A linear pressure programme may be set with a higher pressure at the end of the analysis This can give shorter analysis times and improved peak shapes for later running components In large volume sampl
86. th the default setting at 0 C s To change the Ramp Rate option 1 Use the Up or Down arrow key to move to the required setting Steps are in increments of 1 C s 6 7 6 Final Temperature The Final Temperature option defines the injector temperature at the end of the temperature ramp The range available is between 50 C and 600 C with the default setting at 36 C Page 6 16 Method Menu Optic 2 Issue 1 To change the Final Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 5 C 6 7 7 End Time The End Time option defines the time between the start and end of the GC method run This should be set to the total run time for the GC analysis in order for the cooling of the injector and resetting of pressure parameter to coincide with the GC run end The range is between 0 00 Min Sec and 120 00 Min Sec and Infinite Infinity with the default setting at 0 20 Min Sec To change the End Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 10 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 30 Min Sec 6 7 8 Split Mode The Split Mode option defines the state of the split valve
87. the Up or Down arrow key to move to the required setting Steps are in increments of 1 C s 6 4 6 Final Temperature The Final Temperature option defines the injector temperature at the end of the temperature ramp The range available is between 50 C and 600 C with the default setting at 36 C To change the Final Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 5 C 6 4 7 End Time The End Time option defines the time between the start and end of the GC method run This should be set to the total run time for the GC analysis in order for the cooling of the injector and resetting of pressure parameter to coincide with the GC run end The range is between 0 00 Min Sec and 120 00 Min Sec and Infinite Infinity with the default setting at 0 20 Min Sec To change the End Time option 1 Use the Up or Down arrow key to move to the required time Single presses of an arrow key change the current setting in steps of 0 10 Min Sec Continuous pressure on an arrow key changes the current setting in steps of 00 30 Min Sec Note The following options apply only to the Optic 2 200 and Optic 2 300 6 4 8 Transfer Pressure The Transfer Pressure option defines the pressure in the injector at the start of the method run This e
88. thod 2 Execute Method 2 Method 3 Execute Method 3 Method 4 Execute Method 4 Method 5 Execute Method 5 Configure Menu Password Name Password Control Maximum Temperature GC Ready Input GC Ready Output Contrast Pressure Units Clear Parameters Split Method Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Splitless Method Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Splitless Time Large Volume Method Default Parameters Off 30 C Open End of Sequence 1 Times End of Sequence 1 Times End of Sequence 1 Times End of Sequence 1 Times End of Sequence 1 Times kuki No 600 C Disabled Closed when ready 20 Bar No 01 kkk 0 00 M S 35 C 0 C S 36 C 0 20 M S xg 1 kkk 0 00 M S 35 C 0 C S 36 C 0 20 M S 1 00 M S Appendix C 1 Optic 2 Method Name Equilibration Time Initial Temperature Vent Time Ramp Rate Final Temperature End Time Split Open Time Desorption Method Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Split Mode Expert Method Method Name Equilibration Time Initial Temperature Initial Time Ramp Sub Menu Ramp Rate ISO Temperature ISO Time Split Valve Sub Menu Initial State Split State 1 Time 1 Open Split State 2 Time 2 Open Aux Sub Menu 01 Init State 01 Time 1 01 Time 2 01 Time
89. tial State Split State 1 Time 1 Splitless Split State 2 Time 2 Splitless Aux Sub Menu 01 Init State 01 Time 1 01 Time 2 01 Time 3 01 Time 4 01 Time 5 01 Time 6 01 Time 7 01 Time 8 Carrier Gas Pressure Sub Menu Start Pressure Time End Pressure 0 00 M S Splitless Splitless 0 00 M S Splitless 0 00 M S Open Closed 0 00 M S Open 0 00 M S Closed 0 00 M S Open 0 00 M S Closed 0 00 M S Open 0 00 M S Closed 0 00 M S Open 0 00 M S 0 00Bar 0 00 M S 0 00Bar Auxiliary Gas Pressure Sub Menu Optic 2 300 Start Pressure Time End Pressure Default Parameters 0 00Bar 0 00 M S 0 00Bar Issue 1 Appendix C 5 Issue 1 Optic 2 ifn MALIQ KR d OWEN 02 NOTAM EI uum ALLN METE gjasa senior 1 SHKA GE SMS Old O HOLY Od EJE BEE i IdE OL LE NN 02 adl d LS TL E rana Leo an l EN zi SdWLEZWIOGHI d wr EET uum vidas um NJET Zild N OLH Od duc PH 309 Hd OLLJANN OJ BLER eye B LESA MIONE BUL LINN ewndnoa Ss SL IES yanaswa aa aa Ud N 09 69 HODI NEMI OT WS R HU SL WWE BS Td LN dit T Two SHTO X JE amas d HOH HO Ea ETE MES hes SEL ASEW JZVUH 4O08 OMIT 002 MME nea 2208 d OL LOM diN OL ZS L SEGLI EHECI Appendix D 1 Injector Diagram Optic 2 Rear Panel Pin Connections Note All pins that are not listed in the following tables are not connected Serial Port Connector Issue 1 A 9 way female
90. tive to system ground The current available is limited to approximately 100mA 24V supply This is a voltage of 24V nominal relative to system ground The current available is limited to 250mA This supply is protected by the 250mA fuse located on the rear panel 2 4 4 Pneumatics Optic 2 200 and Optic 2 300 Introduction Page 2 5 Optic 2 Issue 1 Figure 2 3 shows a schematic diagram of the Optic 2 pneumatic controls SS SK SS E SE SS SS K SS I IN E GT OE PHEUMATICS KO priz 2 200 and Ops 23001 lene Maoduk I E F R Elestmanis Frerrure Regular I I I U rm lt woes i 1 P S Prorrur Sensor I H i I Soptum Furgo Heedlo Value Split pilar Sele Flow NAK Value Solenoid Value Vent Flow ens Heedle Valve Zen Br Capilar Column Solenoid Value I OPTIOHAL AUKILIARY PRESSURE CONTROL CHAHHEL I VO pus 2500 Figure 2 3 Pneumatics 2 4 5 Needle Valves and Vents The needle valves and vents are located on the lower front panel of the instrument as illustrated in Figure 2 4 The septum purge flow is set by the Purge needle valve and can be monitored using the port above the needle valve The split line flow is set in the same way using the Split needle valve and port when the split setting is Split and the Vent needle valve and port when the split setting is Vent O O SPLIT VENT PURGE AI CAMBRIDGE PROGRAMMABLE INJECTOR a Figure 2 4 Needle Valves and
91. trol Sub Menu and return to the Expert Menu 1 Press the Menu Exit key Note The following options apply only to the Optic 2 300 6 8 10 Auxiliary Gas Pressure Control Sub Menu The Auxiliary Gas Pressure Control Sub Menu allows the user to set individual parameters for up to four events Each event contains the same three parameters Start Pressure Time and End Pressure To enter the Auxiliary Gas Pressure Control Sub Menu 1 Press the Up or Down arrow key to select the required event 2 Press the Menu Enter key 6 8 10 1 Start Pressure The Start Pressure option defines the level of carrier gas pressure at the start of the event The range is between 0 00 bar 0 0 PSI OkPa and 7 0 bar 101 PSI 700kPa with the default setting at 0 01 bar 0 1 PSI 1 kPa To change the Start Pressure option 1 Use the Up or Down arrow key to move to the required pressure Single presses of an arrow key change the current setting in steps of 0 01 bar 0 1 Method Menu Page 6 27 Optic 2 Issue 1 PSI 1kPa Continuous pressure on an arrow key changes the current setting in steps of 0 10 bar 1 0 PSI 10kPa 6 8 10 2 Time The Time option defines the time that a linear pressure ramp is maintained from the start pressure to the end pressure The range available is between 0 00 Min Sec and 120 00 Min Sec with the default setting at 0 00 Min Sec To change the Time option 1 Use the Up or Down arrow key to m
92. u If the password is incorrect a prompt is displayed to re enter the password B The display shows Injection Mode and Split The first window in all method menus is the Injection Mode window Use the Up or Down arrow key to select the menu required 6 4 Split Menu The Split Method Menu is used to set the following functions and parameters Method Name Equilibration Time Initial Temperature Ramp Rate Final Temperature End Time Transfer Pressure Optic 2 200 Transfer Time Optic 2 200 Initial Run Pressure Optic 2 200 Final Run Pressure Optic 2 200 A graphical representation of these parameters is given in Appendix A 6 4 1 Access the Split Method Menu To access the Split Method Menu 1 Ensure that the display shows Injection Mode and Split Page 6 2 Method Menu Optic 2 Issue 1 2 Use the Left or Right arrow key to select the required parameter 6 4 2 Method Name The Method Name window allows the user to store a customised split method under a name of the user s choice To enter a Method Name 1 Press the Function key The flashing cursor is placed on the first position 2 Use the Up and Down arrow keys to scroll through the alpha numeric characters to select the first character in the name 3 Use the Right arrow key to move the flashing cursor to the next position and repeat step 2 4 Repeat step 3 for the remaining characters in the name If a mistake is made use the Left or Right arrow key
93. ve to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the current temperature in steps of 10 C 4 1 4 GC Reagy Input The GC Ready Input option sets the type of signal Optic 2 must receive to indicate that the GC is ready for an injection If this option is set to Disabled Optic 2 does not wait for the GC before going to the Ready state In all other cases the Optic 2 waits for the GC Ready Relay output to be in the appropriate state before going to the Ready state Refer to the instructions provided with the installation kit for more information To change the GC Ready Input option 1 Use the Up or Down arrow key to select the parameter required either Disabled Open When Ready or Closed When Ready Page 4 2 Configure Menu Optic 2 Issue 1 4 1 5 GC Ready Output The GC Ready Output option sets the type of signal Optic 2 sends to the GC to indicate when the Optic 2 is ready for an injection The signal is sent via a pair of relay contacts The best setting depends upon the type of GC and the system configuration refer to the instructions provided with the installation kit for more information In general the Optic 2 should be a slave to the host GC ie the GC Start Output starts the Optic 2 run In this case the GC Ready Output should be enabled in either Closed When Ready or Open When Ready as appropriate and the GC
94. ys to scroll through the alpha numeric characters to select the first character in the name 3 Use the Right arrow key to move the flashing cursor to the next position and repeat step 2 4 Repeat step 3 for the remaining characters in the name If a mistake is made use the Left or Right arrow key to move to the required position and repeat step 2 5 When all characters are correct press the Function key to store the name 6 8 3 Equilibration Time The Equilibration Time allows the user to provide for improved temperature and pressure stability prior to Optic 2 entering the Ready state The Equilibration Time option defines the time between the temperature and pressure stabilising and the Optic 2 entering the Ready state The range available is between 0 00 Min Sec and 10 00 Min Sec with the default setting at 0 00 Min Sec To change the Equilibration Time option 1 Use the Up or Down arrow key to move to the required time Steps are in increments of 00 30 Min Sec 6 8 4 Initial Temperature The Initial Temperature option defines the injector temperature at the start of the method run The range available is between 50 C and 600 C with the default setting at 35 C To change the Initial Temperature option 1 Use the Up or Down arrow key to move to the required temperature Single presses of an arrow key change the current setting in steps of 1 C Continuous pressure on an arrow key changes the curre
95. yul standard solution obtain a reference chromatogram for comparison purposes 2 CHECK THE LINER PACKING IS INERT WITH RESPECT TO THE SAMPLE Fit a packed liner and inject 1ul of the standard in the cold splitless mode Compare the results with the reference chromatogram Differences between the two chromatograms can indicate non optimised splitless transfer conditions For example If the peak areas tend to be smaller than the reference chromatogram the Optic temperature could be too low or the splitless time is too short If some of the peaks are smaller or absent when compared to the reference chromatogram the liner packing may not be inert to all of the analytes of interest 3 DETERMINE THE LIQUID CAPACITY OF THE LINER VMAX Without turning off the carrier gas remove the column from the injector and inject 150ul of solvent Look for solvent droplets at the injector outlet Progressively reduce the amount injected until no droplets are observed This volume is VMAX 4 DETERMINE THE SOLVENT ELIMINATION TIME Reconnect the column to the injector Set the column temperature to 20 C above the injector temperature Set the detector to minimum sensitivity Set the vent flow to 200cm min Rapidly inject a volume of pure solvent equal to the volume to be used in large volume injection do not exceed 90 VMAX Measure the peak width of the solvent in seconds 5 LARGE VOLUME INJECTION OF STANDARD Dilute the standard by a factor equal to th

User`s Guide for OPTIC 2

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