Home

uProcess User Manual v1.0

1. Sequence idle Ready NUM 5 Figure 9 Control Panel for Valve Manifold Online and Offline The communication status of each device is shown in the uDevices Window To monitor or control a device the device its EIB and the interface must all be online As shown in Figure 10 the icon to the left of each device will indicate whether it is Offline not in communication with the computer or Online in communication In this example the EIB on COM11 is offline and the EIB on COM1 is online To change the status of a device or EIB right click the device in the uDevices Window and check uncheck the Online setting To toggle all connected devices to online or offline click the Online toolbar button Note An EIB will go offline if there is a communication error between the COM port and the EIB Two red lights on the EIB also indicate a communication problem This error 15 typically indicates a bad cable connection The Communications tab of the Output Window lists a history of the EIB port communication status T Untitled uProcess File Edit View Actions Help bea l ae ee ee A uDevices TIX i H Interfaces p EIB on COM11 LabSmith SP501 Addr 27 a LabSmith SP501 Addr 53 G EIB on COMI 8 4 valve Manifold Addr 01 LabSmith SP501 Addr 108 Figure 10 Devices Shown Offline EIB on Com11 and Online EIB on COM1 Device Naming and Numbering Every uDevice syringe pump or valve
2. Device Control Overview Window The previous section explained how to control uDevices through their individual control panels The Device Control Overview window gives you a convenient way to view and set the controls for multiple devices which is particularly useful when setting up the steps of an automated process as we ll show later To view the Device Control Overview window click Interfaces in the uDevices Window Figure 18 eae FIE A 4 Valve Manifold Valve 1 Yalve 2 Valve 3 Valve 4 lopen A Open C Open A Open A C close C close C close C close C Open B Open 8 C Open B Open B Reagent Syringe volume Flow rate 869 So ul min Figure 18 Device Control Overview Window The controls at the top of the window affect all devices Update Continuously updates all devices automatically as you change the settings Apply Settings Now updates all devices to the currently selected settings If Update Continuously is selected this button will not be available x Stop the uDevice stops the action of all devices Updating Continuously will be unchecked Click Monitor All to continuously read the status of all devices Click Monitor None to stop all monitoring Each device will be listed below the main controls To the left of each device are the following two buttons When the Do Not Change uDevice Settings 9 button is depressed no changes will be made for this device when
3. One 20ul syringe named Reagent Syringe One 4VM manifold named Reagent Manifold with one valve connected at position 1 The automated script is created as follows 1 In the uDevices Window select Interfaces to display the Device Control Overview Window Figure 27 33 2 uProcess File Edit View Actions Help Pp p i Reagent Syringe volume Flow rate E of 1 MM o target ul 0 005 ul uljmin T L TO ge eg 21 ul 0 0 1130 Reagent Manifold EE EIB on COM1 Reagent Syringe Addr 07 J Reagent Manifold Addr 12 valve 1 Yalve 2 Yalve 3 Valve 4 Open 4 JOpen 4 JOpen 4 C Open 4 o C close close C close C close C Open B C Open B C Openg OpenB Output 22 01 29 333 Parsing script 22 01 29 333 Parsing succeeded with no errors in O lines 22 01 29 333 All udevices declared in the script are connected 22 01 29 333 The script is ready to run 23 uDevices E Sequencer M 4 gt gt I Script Parsing 4 Sequence Status 4 Communications Sequence idle NUM Figure 27 Installed Devices Shown in Device Control Overview Window 2 Set the position for Valve 1 on Reagent Manifold to Open A oa Set 3 Depress the Do Not Change uDevice Settings button next to the Reagent Syringe Click the Save As a New Sequence Step i button Name the new step Open _Reservoir Select Wait until all devices are finished and Continue Click OK to add this
4. 3 Click the Calibrate button 4 Select OK to store the values Care of the Syringe Glass and Plunger Plunger Care Do not apply excessive force to move a plunger Too much pressure can irretrievably bend the plunger or easily crack the syringe s glass barrel Avoid touching the plunger with your fingers Abrasions scratches or oil due to handling the plunger with your fingers may interfere with proper plunger operation To minimize the chance of damage or contamination do not store the plunger outside the glass Use caution when reinserting the plunger into the barrel using the plunger installation tool For best results dip the PTFE plunger tip into your solvent to re wet it prior to reinserting the plunger into the barrel Cleaning Sterilizing Autoclaving and Disinfecting Cleaning To clean syringes it is best to use solvents known to be effective in solvating the sample Preferred cleaning agents are non alkaline non phosphate and non detergent based A biodegradable non phosphate organic Cleaning Concentrate is available from Hamilton Part No 18311 Rinse the syringe after use with an appropriate solvent or cleaning agent Following the use of a cleaning agent rinse the syringe with deionized water and finally acetone Wipe the exterior surfaces of the syringe barrel and needle dry with a lint free tissue Make sure that there is no residual cleaning agent in the syringe before using or storing the syringe D
5. Break lt Break gt automatically activates the pause 4 toolbar button Sequence pauses until the pause toolbar button is pressed This function is useful for testing a script or if you have an event with unpredictable timing that you need to wait for before resuming a script Example lt Break gt Note The Break function cannot be added using the Process Wizard Quit lt Quit gt quits the script steps following this function will not be run This function will not stop a device that is already in process for that use the Stop function Example lt Quit gt Note The Quit function cannot be added using the Process Wizard General Formatting Rules Devices Individual devices are defined in the script as follows Syringes DeviceName SPS01 size ul Where size is the syringe size 4 8 20 40 or 80 Valve Manifolds DeviceName 4VM01 DeviceName must match name defined in the uDevice configuration see Device Naming and Numbering Comments Comments can be added to the script using a semicolon Anything after the semicolon is treated as a comment lt Pause gt 1000 ms to allow time to monitor reaction Step Name Step names may have a maximum of 16 characters and may not include blanks or lt gt or Edit Automated Script Text As mentioned earlier you can create a script using the Process Wizard or you can create edit the text directly 29 To view and edit t
6. The uProcess Dashboard consists of a menu bar toolbar and configurable windows Figure 3 Unique menu and toolbar commands will be described in the body of the text below A complete listing of all menu and toolbar commands can be found in Appendix A Menus Toolbars Window Controls Untitled wProcess File Edt View Actions Help JG lanl a uDevices i 3 Interfaces uDevices Window Main Window Sequencer Window Tab IB ubevices HET nce Output Output Window Wod M Sop Parsing Sagene Salus To F Sequence idle Heady Figure 3 uProcess Dashboard The uProcess Dashboard consists of 5 windows described below You can customize the placement of the windows on the dashboard and a number of other features about the interface See Customizing the Dashboard for more on customizing the window layout uDevices Window The uDevices Window is used to view add and configure individual devices and EIBs Figure 4 It shares the left side of the Dashboard with the Sequencer Window Click the tabs at the bottom of this area to switch between the two windows 11 uDevices q i3 m E a Interfaces G EIB on COM3 Reagent Syringe 4ddr 02 4 Valve Manifold 1 4ddr 04 Sample Syringe Oddr 16 ay Sequencer uDevices Figure 4 uDevices Window Sequencer Window The Sequencer Window is used to view a sequence program Figure 5 The uDevices Window is used to view add and configure in
7. wd Labomith TOOLS FOR SCIENCE LabSmith uProcess System User Guide Version 1 0 For Operation and Control of LabSmith Programmable Syringe Pumps and Automated Valves using uProcess Software This manual 2011 LabSmith Inc uProcess software 2011 LabSmith Inc No part of this document may be reproduced or distributed without the consent of LabSmith LabSmith CapTite uProcess and uDevice are trademarks of LabSmith Inc TABLE OF CONTENTS CHAPTER 1 INTRODUCTION Equipment List Required Equipment Optional Equipment Cautions and Warnings Getting Help About This Manual CHAPTER 2 GETTING STARTED Connecting an Electronic Interface Board Adding a Breadboard Installing Automated Valves Installing Syringe Pumps Installing uProcess Software CHAPTER 3 UPROCESS SOFTWARE Software Interface uDevices Window Sequencer Window Output Window Main Window Communicating with the uDevices Online and Offline Device Naming and Numbering Device Address Device Name Adding or Removing uDevices CHAPTER 4 REAL TIME DEVICE CONTROL Device Control Panel Common Controls Syringe Pump Control Panel Current Syringe Pump State Position Control Speed Control Optimizing Syringe Pump Performance Valves Control Panel Current Valve State Device Control Overview Window Syringe Pump Settings Automated Valve Settings CHAPTER 5 AUTOMATED CONTROL Opening Script Files Create a Script Using the Wizard Save a Scrip
8. Tooltips explain a tool s function Choose View gt Verbose Tooltips to view more extensive descriptions Figure 35 O Stop All uDevices and Automation Stop all uDevices and stop any running sequence Figure 35 Tooltips Provide Helpful Tool Descriptions Customizing the Dashboard Show Hide Dashboard Elements From the View menu choose Toolbars and Docking Windows From this menu option you can choose to show or hide the toolbar uDevices Window Sequencer Window and Output Window Auto Minimize Windows Click the push pin button m at the upper right of the uDevices Window Sequencer Window or the Output Window to minimize the window to the edge of the screen Roll over a minimized window to make it reappear it will automatically minimize when you roll the cursor off of the window The push pin button will be horizontal in this auto minimize mode Click the button again to turn it vertically and disable auto minimizing Detach Dock and Re Position Windows Click and drag the top of the uDevices Window Sequencer Window or the Output Window to detach it from the Dashboard and create a floating window Drag the floating window over the 42 Dashboard to dock it Positioning guides will appear as you do so to help you attach the window at the top bottom left or right of the uProcess Dashboard Figure 36 AOO m Windows XP 1 Parallels Desktop 2 oy We O E BO TESI m uDevices N x Fo
9. or to a USB port with optional RS232 to USB adapter aan Equipment PC compatible computer running Windows XP NT or 7 uProcess software installation disk included Electronic Interface Board EIB 12V power supply for EIB RS232 serial interface cable 20 pin communication cable Optional Equipment UPB or iBB breadboard RS 232 to USB adapter cable SPS0O1 Programmable Syringe Pumps SPS0O1 syringe glass plunger set Plunger installation wrench hex wrench syringe installation screws M3 label stickers AV201 automated valve 4VM4 valve manifold one manifold per up to four valves Ribbon connector Valve installation screws 2 28 Cautions and Warnings When working with uProcess devices and accompanying electronics follow standard procedures to protect your devices from excessive static electricity power fluctuations etc Read and understand all documentation for your individual uProcess devices and components before connecting the equipment CAUTION Extra care must be taken when using the electronic components with fluids Breadboards are designed with the electronics on the left side take care not to introduce liquids to this side of the breadboard Getting Help This guide is your main source for information on operating uProcess software Choose Help gt About uProcess then click the Open User Manual link to browse this manual electronically in Adobe Acrobat PDF format The
10. 0 0 7 2 Sequencing Script lt WaitAllDone gt H Reagent Syringe SPS501 20 ul Fill Syringe ot Reagent Manifold 4 M01 Reagent Syringe SetFlowRate 50 000 ulmin Open_Reservoir Reagent Syringe MoveTo 10 000 ul iam lt Wait llDone gt e Reagent Manifold SetValves 1000 Open Process t lt WaitAllDone gt Reagent Manifold SetValves 3 0 0 0 HE Fill_Syringe lt Pause gt 1000 ms ey Deliver_Reagent eae ee glans me allel Reagent Syringe SetFlowRate 10 000 ul min a Reagent Syringe MoveTo 0 000 ul AF lt WaitallDone gt lt WaitAllDone gt fe Open_Process lt Loop gt Fill Syringe 10 Reagent Manifold SetValves 3000 H lt Pause gt 1000 ms Deliver_Reagent Reagent Syringe SetFlowRate 10 000 ul min f Reagent Syringe MoveTo 0 000 ul H lt WaitallDone gt lt Loop gt Fill_Syringe 10 Output 22 18 44 958 Parsing script 22 18 44 958 Parsing succeeded with no errors in 17 lines 22 18 44 958 All udevices declared in the script are connected 22 18 44 958 The script is ready to run rr 3 TATER N a s AE SALTI T a OANA ENEA ae ALN 25 uDevices Sequencer M 4 gt gt I Script Parsing 4 Sequence Status A Communications Sequence idle Ready AP NUM SCF Figure 32 The Complete Example Script 23 Press the Run button to initiate the automated script When a script is running the current step will highlighted in the Main Window to allow tracking of the script progress The status of the device
11. Reservoir Reagent Manifold WaitAllDone gt Fill Syringe Reagent Syringe Reagent Syringe WaitAllDone gt Open Process Reagent Manifold Pause 1000 ms Deliver Reagent Reagent Syringe Reagent Syringe WaitAllDone gt Loop Fill Syringe s Output 226 39 630 Parsing script Bele SPS01 20 ul 4VMO1 SetValves 1 0 0 O SetFlowRate 50 000 ul mii MoveTo 10 000 ul SetValve s 3 0 0 O SetFlowRate 10 000 ul mii MoveTo 0 000 ul 10 222 39 630 Error on line 6 Unexpected characters found Syringe 2226 39 630 Eror on line 17 Label not found Fill Syringe eer a9 630 Parsing tailed with 2 errors in 17 lines 222 39 630 Allubevices declared in the script are connected fe 2F 99 630 The script cannot be run until the script eror are resolved N 4 H 3 Seript Parsing 4 Sequence Status NUM Figure 23 Sample script showing errors in Lines 6 and 17 Missing Devices All of the devices referenced in a process must be available for the process to function If you open a script which calls out a device that is not present error messages will appear in the uDevices Windows the Script Parsing tab of the Output Window and the Main Window Figure 24 31 P uProcess File Edit View Actions Help MEN ed hs i b uDevices EIB on COMI Syringe 1 Addr 07 Syringe 1 4 Reagent Manifold Addr 12 vone FATE Missing devices target 0 000 u 000 ul 10 000 ulmin P Reage
12. default ul s ni min nl s Example Reagent SetFlowRate 50 ul s MoveTo SyringeName MoveTo vol units sets syringe move to position SyringeName defined name of syringe must be defined in script header vol syringe volume to move to 2 units volume units acceptable units include ul default ml nl Example Reagent MoveTo 20 5 ul Stop DeviceName Stop _ stops device DeviceName_ defined name of 4VM or syringe must be defined in script header Note The Stop command is added via the Process Wizard using the x button Stop this uDevice in the Device Control Overview window Example Reagent Stop Pause lt Pause gt duration units pauses script for specified duration Duration length of pause units pause duration units acceptable units include ms default S uS Example lt Pause gt 1 s WaitAllDone lt WaitAllDone gt pauses script until all processes are complete Example lt WaitAllDone gt WaitDone lt WaitDone gt DeviceName _ pauses script until all defined device is complete DeviceName_ defined name of 4VM or syringe must be defined in script header Example lt WaitDone gt Reagent Note The WaitDone function cannot be added using the Process Wizard Loop lt Loop gt StepName cycles _ loops back to named step for specified number of cycles SteoName defined name of step in script cycles number of times to loop Example lt Loop gt FillStep 6 28 Note leave blank for endless loop
13. manifold is identified by an address and name Note Clicking on the device name in the uDevices Window will cause the indicator light on the device to flash red green This feature aids in quickly identifying each device Device Address Each device attached to an EIB must be assigned a unique address between 0 and 110 If you purchased several devices at the same time from LabSmith they will be preconfigured with unique addresses Device addresses are shown next to each device in the uDevices Window and also on in the Address field of the Device Control Panel Note If multiple devices on the same EIB have identical addresses they may not appear in the uDevices Window list or they may appear as one device Operating in this condition can cause unpredictable results If two devices with the same address are installed one will need to be removed before the address can be changed Note The address assigned to each device is independent of its physical position on a breadboard On the control panel for each EIB devices will be displayed in numerical order based on the device address not their position on the breadboard To change a device address 1 Select the device in the uDevices Window 2 Choose Configuration 3 Select a Device Address from the dropdown menu and click OK You can also right click on the device icon in the uDevices Window and choose Change Address Tip Use the included yellow labels to mark the device addr
14. so the syringe won t stop prematurely lt WaitAllDone gt Open Process Reagent Manifold SetValves 3 0 0 0 lt Pause gt 1000 ms Deliver Reagent Reagent Syringe SetFlowRate 10 000 ul min Reagent Syringe MoveTo 0 000 ul lt WaitAllDone gt this command is commented out lt Pause gt 60 s allow the syringe to run for 60 s Reagent Syringe lt Stop gt stop the syringe lt Loop gt Fill Syringe 10 39 40 APPENDIX A MENUS TOOLBARS WINDOW CONTROLS Menu Bar The menus contain the following functions File Edit View Actions Open Close Save Print or create a new automated process Cut copy or paste text while editing an automated process Choose which uProcess windows and toolbars are displayed Run Pause or Stop an automated process control hardware communications and update firmware Click and drag the three dots at the left of the menu bar to tear it from the main window as a floating palette Click and drag the floating palette back into the main window to re dock it Toolbar Toolbar buttons provide one click shortcuts to common tasks Buttons are gray when those functions are not currently available Ee id Ba d New ctrl n ad Open ctrl o a Save ctrl s ry Cut ctrl x Ja Copy ctrl c a Paste ctrl v Print ctrl p About uProcess p ll La Run Pause Quit po gt n ao Figure 34 uProcess Toolbar Buttons Create a new automated process Open an ex
15. to construct edit and monitor sequence programs Its contents change depending upon what is selected in the uDevices Window or Sequencer Window The Main Window cannot be undocked or hidden 13 Communicating with the uDevices Once the uDevice hardware and uProcess software have been installed you are ready to communicate with the devices Launch the uProcess software Right click on Interfaces in the uDevices Window and choose New Interface Select the computer port to which the EIB is connected and click OK If multiple EIBs are installed repeat this step for each EIB Your uProcess dashboard should now look similar to Figure 7 X Untitled uProcess File Edit View Actions Help Joa 4 A uDevices 3 a Interfaces S EIB on COM11 LabSmith 5P501 Addr 27 LabSmith SP501 Addr 53 Se EIB on COMI B 4 Valve Manifold Addr 01 LabSmith SPSO1 Addr 108 LabSmith SPS01 Volume target 6 9 Flow rate 54 ul 0 46 LabSmith SPS01 Volume Flow rate target B i J uljmin J 4Valve Manifold valve 1 Valve 2 Valve 3 Valve 4 Open Clopen Clopen Clopen C close C close C close C close Clopen 6 Cl open 6 Cl open B C Open 6 54 ul 0 46 LabSmith SPS01 volume Flow rate target ul min P ay 54 ul Ea uDevices Jas Sequencer Output M 4 gt gt Script Parsing 4 Sequence Status Z Communications F Sequence idl
16. 2 Duration fms 100 Slew Current 135 Duration fms 10 End Current 151 Duration ims 250 default Firmware Hardware version 02 00 00 05 Firmware version 00 04 Firmware up bo date Figure 37 Configure Device Dialog Box for a Syringe Pump and Valve Manifold Changing the Syringe Pump Glass Assembly The SPS01 syringe glass and plunger can be removed from the pump body to be cleaned or exchanged with a different syringe size To remove the syringe glass and plunger 1 Supply power to the syringe pump 2 Inthe uProcess uDevices Window select the syringe 45 3 Enter a Set Point of 0 to extend the syringe plunger to the 0 volume position Figure 38 Reagent Syringe x Y Pump Properties Sons f Online V Monitor 2 Click Apply to extend plunger 2 Syringe Size Standard 20 ul Speed Control ul min lt J 0 186 i j 1130 Position Monitoring amp Control ce ul J 0 000 ul 21 ul 00u g s ul Set Point Actual 1 Enter 0 Setpoint Figure 38 Set the Set Point to 0 and click Apply to extend the plunger Click Offline to take the syringe offline Unplug the syringe from the breadboard Using the supplied hex wrench loosen the screw located on the top side of the syringe Figure 39 Figure 39 Loosen the Retaining Screw 7 Gently pull the syringe glass straight out of the syringe body being careful not to bend the syringe plunger 8 While h
17. All steps will be performed in order While a script is running you can click the Pause t toolbar button to pause the automated process Click Pause again to continue the script from that point 26 Click the Quit Process ad toolbar button to quit running the automated script Note When you Pause or Quit a script devices that were moving will continue until they O reach their target states Click the Stop All Devices __ _ toolbar button to stop all device activity While an automated script is running the Sequence Progress tab of the Output Window will indicate the current status of the script and will report any errors encountered Figure 22 Output 20 42 21 875 Script Message Script started 2042 21 05 Scnpt Running Fill Syringe 20 44 26 640 Script Running Deliver Reagent Figure 22 Sequence Progress Tab of the Output Window Automated Script Functions SetValves ManifoldName SetValves x x x x sets valve positions ManifoldName defined name of 4VM must be defined in script header XXXX position of valves 1 2 3 and 4 respectively where x 0 no change X 1 open valve to A x 2 close valve x 3 open valve to B Example 4vM A SetValves 10 3 2 SetFlowRate SyringeName SetFlowRate rate units sets syringe flow rate SyringeName defined name of syringe must be defined in script header rate syringe flow rate positive number units flow rate units acceptable units include ul min
18. Do nok wait then Continue End if this is the last step T times 0 unlimited Cancel Figure 29 Step 1 Settings 13 Click OK to add this step to the script To view the process thus far click the Sequencer Window tab then click on any step in the sequencing script Figure 30 Select Interfaces in the uDevices Window to return to the Device Control Overview Window and continue building the script 35 36 2 uProcess File Edit View Actions Help OF EET Oh a EE Sequencer vaceee Reagent Syringe SPSO1 20 ul Reagent Manifold 4VM01 gt i Open_Reservoir e x x Reagent Manifold Set Valves 10 0 0 7 Sequencing Script lt WaitAllDone gt et Reagent Syringe SP501 20 ul Fill Syringe et Reagent Manifold 4V MO1 Reagent Syringe SetFlowRate 50 000 ul min Open_Reservoir Reagent Syringe MoveTo 10 000 ul lt Wai gt Reagent Manifold SetValves 1000 Sues Done H lt WaitAllDone gt Fill_Syringe 2 Reagent Syringe SetFlowRate 50 000 ul min E Reagent Syringe MoveTo 10 000 ul HE lt WaitAllDone gt Output 22 09 19 271 Parsing script 22 09 19 271 Parsing succeeded with no errors in 3 lines 22 09 19 271 All uDevices declared in the script are connected 22 09 19 271 The script is read Fa uDevices Sequencer M 4 gt gt Seript Parsing 4 Sequence Status 4 Communications 14 15 16 17 18 19 20 21 Sequence idle Ready NUM Fi
19. LabSmith web site www labsmith com also includes helpful applications notes and other technical information to help you use uProcess software and devices If you are unable to find the information you need please contact our technical support team at 925 292 5161 or email us at support labsmith com Be prepared to give the software version To determine the software version number select Help gt About uProcess About This Manual Several conventions are used throughout this manual to highlight important features and concepts Menu gt Option Menu bar selections will be shown in bold text with descending options separated by the gt symbol For example Help gt About uProcess indicates that you should click the Help menu and then click About uProcess Note Notes indicate helpful tips or additional information CAUTION These notes signify important considerations that will help you to avoid losing data or damaging hardware Italics When browsing the electronic version of this manual click on these italic cross references to view other pertinent sections of the manual Choose Help gt About uProcess then click the Open User Manual link to browse the electronic version of this manual CHAPTER 2 GETTING STARTED Connecting an Electronic Interface Board Though the devices you use with your uProcess system will vary some basic hardware is common to all uProcess setups Every system requires at least one Electronic Interfac
20. ample the syringe is set to deliver 10ul of reagent at 10ul min In order not to overshoot the delivery of the reagent the syringe will slow down just before reaching the target volume therefore the delivery time may be more than 1 minute This is most pronounced when delivering at high flow rates For some processes the infuse time may be more critical than the total delivery volume For this case you can use the lt Pause gt and lt Stop gt functions together to set the infuse time The script from the above example is shown below Reagent Syringe SPSO1 20 ul Reagent Manifold 4AVMO1 Open Reservoir Reagent Manifold SetValves 1 0 0 0 lt WaitAllDone gt Fill Syringe Reagent Syringe SetFlowRate 50 000 ul min Reagent Syringe MoveTo 10 000 ul lt WaitAllDone gt Open Process Reagent Manifold SetValves 3 0 0 0 lt Pause gt 1000 ms Deliver Reagent Reagent Syringe SetFlowRate 10 000 ul min Reagent Syringe MoveTo 0 000 ul lt WaitAllDone gt lt Loop gt Fill Syringe 10 38 The total volume delivered is 10ul which at 10ul min should take approximately 1 minute If a delivery time of exactly 1 minute is desired the script could be modified as follows changes are in bold Reagent Syringe SPS01 20 ul Reagent Manifold AVMO1 Open Reservoir Reagent Manifold SetValves 1 0 0 0 lt WaitAllDone gt Fill Syringe Reagent Syringe SetFlowRate 50 000 ul min Reagent Syringe MoveTo 11 000 ul provide extra volume
21. dividual devices and EIBs It shares the left side of the Dashboard with the Sequencer Window Click the tabs at the bottom of this area to switch between the two windows 12 Sequencer AX be Sequencing Script Reagent Syringe SP501 80 ul 4 Valve Manifold 4VM01 Fill_Syringe 4 Valve Manifold SetValves000 1 Reagent Syringe SetFlowRate 50 000 ul min Reagent Syringe MoveTo 105 574 ul lt WaitAlilDone gt Deliver_Reagent 4 Valve Manifold SetValves 0003 Reagent Syringe SetFlowRate 10 000 ul min Reagent Syringe MoveTo 0 000 ul lt WaitAllDone gt i Sequencer fl MI Figure 5 Sequencer Window Output Window The Output Window is used to monitor device communications or to debug and monitor a sequence program Figure 6 By default this window appears at the bottom of the dashboard 15 57 36 357 Parsing script 18 57 38 357 Parsing succeeded with no errors in 0 lines 15 57 38 357 All uDevices declared in the script are connected 15 57 38 357 The script is ready to run Figure 6 Output Window Showing Script Parsing Tab The Output Window contains three tabs Script Parsing displays any errors in the current automated process Sequence Status indicates which process is running and notes any errors incurred Communications shows the status and history of communication between the Electronic Interface Board and the computer Main Window The Main Window is used to control and monitor devices as well as
22. e Board EIB which can control up to 110 SPS01 Syringe Pumps and or 4VM Valve Manifolds Since each valve manifold can control up to four valves one EIB can thereby control hundreds of devices Although over 100 devices can be sequentially controlled on an EIB only 10 15 devices can be actuated simultaneously More than one EIB can be connected to the computer at one time when a very large number of devices or concurrent operations are required To connect an EIB to the computer Connect the serial interface cable from the COM port on the EIB to the computer s RS232 port An optional RS232 to USB adapter allows you to connect the EIB to a USB port instead Connect the power transformer from the EIB to a wall outlet The two indicator lights will flash when powered up after which the Power light will stay illuminated red The COM light will illuminate when the EIB is communicating with the computer Adding a Breadboard An EIB can be connected to a single syringe pump or valve manifold however it is typically used with one of the LabSmith breadboards uPB 5 uPB 8 iBB for mounting multiple components Figure 2 Between 5 and 8 uDevices syringe pumps or valve manifolds can be connected to a single breadboard The uPB 8 and iBB breadboards can be daisy chained together by connecting a ribbon cable from the female connector on the left side of the board to the male input on the next board Figure 2 uPB 05 and uPB 08 Breadboards wi
23. e Ready Figure 7 uProcess Dashboard The uDevices Window contains a tree structure listing each EIB and the devices installed under each In Figure 7 two EIBs are connected Two SPSO1 syringe pumps are connected to the EIB on COM11 A4VM and a SPSO1 syringe pump are connected to the EIB on COM1 The devices connected to a particular EIB can be hidden by clicking the button next to the EIB name in the uDevices Window Click the button next to the EIB to display its devices Note Breadboards will not appear as device in the uDevices tab since there are no functions to control on the breadboard itself The Main Window will show one of two views The default view is the Device Contro Overview Window which can also be viewed by clicking Interfaces in the uDevices Window The Device Control Overview allows limited control of all devices It is also the view that will be used to program sequences using the programming wizard see Create a Script Using the Wizard By clicking on one of the individual device names syringe pump or 4VM in the uDevices Window the Individual Device Control panel opens Figure 8 shows the control panel for a syringe pump while Figure 9 shows controls for a 4VM valve manifold 14 P uProcess File Edit View Actions Help Dea ee eee eee ee S uDevices Bz LabSmith SPS01 a Interfaces Pump Properties TE ey A i S EIB on COM11 Address 27 Syringe Size Standard 40 ul Config
24. elect the correct glass size 7 Click on the device in the uDevices Window 8 Select Configuration 9 Choose the syringe pump glass size from the Size drop down list 10 Follow instructions for calibrating below CAUTION Ensure you are using the correct size plunger for your syringe glass Calibrating the Syringe Pump Each SPS01 syringe pump is calibrated before shipment However each glass plunger assembly may have slight length variations up to 1 and there can also be variations in the placement of the glass in the syringe body Each time the syringe glass is removed or moved the syringe pump should be recalibrated Calibrating the syringe pump allows uProcess to find the plunger stopping point thereby minimizing the dead volume within the syringe and allowing correct calculation of the syringe volume Note Calibrating the syringe will run the syringe to its maximum extents and will update its calibration values The procedure may take up to 1 minute To calibrate the syringe 1 Select the device in the uDevices Window 2 Select Configuration to open the Syringe Pump Configuration dialog box Figure 42 47 Configure LabSmith P 01 Device Address custom Size 230 mm diameter Calibration Full uk 1070 Full In botla Calibrate Firmware Hardware version 01 02 00 05 Firmware wersion 01 15 Update Firmware Firmware up to date Figure 41 Syringe Pump Configuration Dialog Box
25. ess on each syringe pump or valve manifold for easy identification 16 Device Name The default device name for the syringe pump is LabSmith SPSO1 The default device name for the 4VM is 4 Valve Manifold You can rename the devices The name may have a maximum of 16 characters and may not include lt gt or Names may include tabs and spaces but may not start or end with them Names are case sensitive If you will be using automated processes each installed device name must be unique To change a device name 1 Select the device in the uDevices Window 2 Choose Configuration 3 Type the new device name in the Name field You can also right click on the device icon in the uDevices Window and choose Rename Adding or Removing uDevices To add or remove a device 3 Click the Online toolbar button Oo to toggle all interfaces offline Physically add or remove the uDevice s to or from the breadboard Click the Online toolbar button to toggle all interfaces back online Click the Rescan toolbar button to check for new devices Any added devices should now appear in the uDevices Window while any removed devices will be gone Note To rescan an individual EIB right click its icon in the uDevices Window then choose Rescan Devices 17 18 CHAPTER 4 REAL TIME DEVICE CONTROL uProcess lets you control connected devices manually or as part of an automated process D
26. evice Control Panel When you click on a device in the uDevices Window its Device Control Panel will appear in the Main Window as shown in Figure 8 and Figure 9 Common Controls All devices have the following commands in common Figure 11 9 S Configuration Figure 11 Controls Common to All uDevice Control Panels Configuration Opens the Configure dialog box for the device Detailed descriptions of the configuration options are described in Device Naming and Numbering Changing the Syringe Pump Glass Assembly and Calibrating the Syringe Pump Online When this box is checked uProcess will send commands and receive data from the device The EIB to which the device is connected must also be online Uncheck the box to take the particular device offline Monitor When this box is checked uProcess will continuously read and display the device s status Uncheck the box to stop monitoring activity for the device O Apply Settings Now updates the uDevice to the selected settings Update Continuously updates the uDevice continuously as the settings change x Stop the uDevice stops the current action of the device and deselects the Update Continuously button Note Pressing the Stop button in a Device Control Panel affects only the selected device To stop all devices use the Stop All uDevices IO toolbar button or the Stop the uDevices button in the Device Control Overview Window Syringe Pump Control Panel In the uDe
27. gent Syringe SPSO1 80 ul Valve Manifold 1 4VM01 Fill Syringe Valve Manifold 1 SetValves 0 0 OU 1 Reagent Syringe SetFlowRate 50 000 ul min Reagent Syringe MoveTo 100 ul Waita Al lLDone gt Deliver Reagent Valve Manifold 1 SetValves U00 3 Reagent Syringe SetFlowRate 10 000 ul min Reagent Syringe MoveTo 0 000 ul lt Waita Al lDone gt Figure 20 Text of an Automated Process Create a Script Using the Wizard The Process Wizard provides a simplified interface for quickly generating automated scripts Note Each installed syringe pump and 4VM must have a unique name regardless of whether it is used in an automated script See Device Name To create a script using the Process Wizard 1 Click the New toolbar button or choose File gt New If a process is currently open its contents will be copied into this new script Select the uDevices Window Click Interfaces to view the Device Control Overview Window Set the initial target state for each device 5 Click the Save as New Sequence Step button The Creating a New Sequence Step dialog box will open Figure 27 25 Creating a new sequence step New step name Stepi After changing the ubevice settings C Pause 4000 ms wait until all devices are Finished O Do not wait then O Continue End if this is the last step ooo times 0 unlimited i Cancel Figure 21 Creating a New Process Step Dialog Box 6 If desired enter a descripti
28. gure 30 Step 1 Shown in the Sequence Tab and Main Window Set the position of Valve 1 to Open B and depress the 9 button for the Reagent Syringe Click the Save As a New Sequence Step button and name the new step Open Process Choose Pause and set the time for 1 s 1000 ms Choose Continue and select OK to go on to the next step O button Set the Target Volume to 0 and the Flow Rate to 10ul min and depress the for the Reagent Valve Click the Save as a New Sequence Step button Name the step Deliver _Reagent Select wait until all devices have finished Select Loop to Step and choose Fill Syringe from the drop down menu Set the loop count to 10 and the process will repeat for 10 iterations Figure 37 Creating a new sequence step Mew step name Deliver_Reagent After changing the ubevice settings C Pause 4000 ms Wait until all devices are finished C Do not wait then O Continue End if this is the last step Loop to step Fill_ Syringe times 0 unlimited Figure 31 Deliver_Reagent Step Settings 22 Click OK Again select the Sequencer Window tab and click on a step in the script to view the complete script Figure 32 P uProcess File Edit View Actions Help Bea Ree eee eee ON eee ee G 2l Sequencer v ax Reagent Syringe SPS01 20 ul Reagent Manifold 4 MO1 ii Open_Reservoir Reagent Manifold SetYalves 10
29. h Please refer to the capillary or tubing packaging for cutting instructions 2 Insert the capillary into the nut end of the one piece fitting until the tubing extends through the tapered ferrule end approximately 2 mm 3 Insert the one piece fitting into the component and turn the fitting to finger tighten CAUTION Do not over tighten the fitting Fittings should only be finger tight to avoid damage the 1 8 hex wrench can be used to tighten or loosen difficult to reach fittings 4 Gently pull on the capillary tubing to ensure that it is held securely by the fitting If the capillary comes free loosen the fitting and repeat steps 2 and 3 5 If the fitting leaks or the capillary cannot be secured the following troubleshooting items should be checked prior to further tightening the fitting a The capillary may be cut too short and so it is not protruding through the end of the fitting when installed b The capillary may not have a clean cut An angled or jagged edge will cause the fittings to leak c Broken capillary or other debris may be stuck inside the fitting or component Try flushing the port from the opposite side to clear the debris 6 If pressures greater than 10 000 psi are required first finger tighten all fittings and test for leaks at low pressure Then use the 1 8 hex wrench to tighten the fittings approximately 1 4 extra revolution 49 Figure 42 shows capillaries that are properly and
30. he text of a script click on the Sequencer window then select Sequencing Script The text will appear in the Main Window Here is a sample script Reagent Syringe SPSO1 20 ul Reagent Manifold AVMO1 Open Reservoir Reagent Manifold SetValves 1 0 0 0 lt WaitAllDone gt Fill Syringe Reagent Syringe SetFlowRate 50 000 ul min Reagent Syringe MoveTo 10 000 ul lt WaitAllDone gt Open Process Reagent Manifold SetValves 3 0 0 0 lt Pause gt 1000 ms Deliver Reagent Reagent Syringe SetFlowRate 10 000 ul min Reagent Syringe MoveTo 0 000 ul lt WaitAllDone gt lt Loop gt Fill Syringe 10 The first lines define the devices used in the script Note that the devices are defined by name rather than address so the names of all devices must be unique Below these declarations each step is listed in order For clarity all state changes within each step are indented below the step name When learning to edit the text it may prove helpful to create a script using the Wizard then to edit the generated text as the basis for your new script Most standard text editing functions are available including Cut Copy and Paste Other Sequence Script Functions Print Script Text Click the Print toolbar button to print out the text of the current process Script Errors uProcess will review the script as you create or edit it and will indicate errors as follows 1 Ifthe script has an error the Run button will be grayed out and the sc
31. improperly installed Red capillary does not protrude through the one piece fitting The fitting will leak and also there is a chance of compressing and damaging the tip of the one piece fitting during installation Yellow capillary may properly seal but the capillary is not fully seated in the interconnect creating dead volume Green capillary is properly installed Figure 42 T Interconnect Showing Properly Installed Capillary Green Improperly Seated Capillary Yellow and Capillary Not Protruding Through Fitting Red 50 51
32. isting automated process Save the current automated process While editing an automated process delete selected text and place it on the clipboard While editing an automated process copy selected text to the clipboard While editing an automated process paste text from the clipboard Print the text of an automated process Find the uProcess version number This window also contains links to the electronic version of this User Manual the LabSmith web site and LabSmith tech support Control the progress of an automated process 41 O Stop all devices Stop all activity of all uProcess devices J L a id Re scan devices Check for all connected uProcess devices Online Establish communication between the EIB and computer Tip You can also use keyboard shortcuts shown in parentheses in the list above to perform the commands To toggle toolbar buttons on or off click the down arrow on the right side of the toolbar then choose Add Remove Buttons gt Standard Choose Reset Toolbar to show all buttons Click and drag the three dots at the left side of the toolbar to tear it from the main window as a floating palette Click and drag the floating palette back into the main window to re dock it Choose View gt Toolbars and Docking Windows gt Standard to show or hide the toolbar Tooltips Each toolbar button and many other buttons in uProcess includes tooltip text that appears when you position your cursor over the button
33. izing Syringe Pump Performance The syringe pump is designed for optimal accuracy in the push infuse direction The pump motor design includes an anti backlash spring which can cause minor volume inaccuracy in the pull direction In addition when changing the syringe direction from pull to push or vice versa you may notice a slight delay before the syringe plunger starts advancing The volume inaccuracy and duration of the delay will vary depending on the syringe size set speed system back pressure and fluid viscosity To improve the timing and volume accuracy of an infusion we recommend pushing a small volume of fluid prior to starting the timed infusion For example if you want to pull 10 ul of fluid from a reservoir and then push infuse it into a process we recommend pulling 11 ul of fluid then push 1ul back into the reservoir before switching your valve to the infuse position Valves Control Panel Click a valve manifold in the uDevices Window to view its control panel Figure 16 21 Valve Manifold 1 Q Four Valve Driver Properties el Online Monitor Valve 1 Valve 2 Valve 3 Valve 4 C No change No change No change No change aa Oa Oa Oa O Closed dosed C closed Closed On Os OB OB In position Indeterminate Indeterminate Indeterminate Figure 16 Controls for a 4VM valve manifold and AV201 automated valves Note All 4 valve positions on a 4VM will be displayed regardless of how many valves are actua
34. le Syringe Pumps deliver volumes up to 100 microliters with resolution as low as 2 nanoliters The pump body design allows syringes to be easily removed and replaced providing a wide range of volumes with a single pump AV201 Automated Selector Valves provide 2 position 3 port switching with zero dead volumes and low swept volumes uProcess Software provides a simple interface for configuring and controlling uProcess devices and for creating automated processes for coordinated control This user guide will show you how to connect configure and control uProcess devices how to install and operate uProcess software and how to create automated flow control processes Please contact LabSmith or visit www absmith com for user manual software and firmware updates Equipment List uProcess software communicates with uProcess devices through the Electronic Interface Board EIB The EIB is typically connected via communication cable through one of the LabSmith breadboards uPB iBB etc enabling control over multiple devices Figure 1 Communication 4VM Valve Manifold AV201 Automated Valves EIB FPSO meqensies Sines Pore ad a SPS01 Syringe Pumps uPB 08 Breadboard Figure 1 uProcess System Components The EIB can also connect directly to a single SPSO1 syringe pump or to a 4VM valve manifold to operate up to four AV201 valves without a breadboard The EIB connects to the computer via the RS232 cable
35. lly installed In addition to the Common Controls described earlier you can view perform the following for each connected valve No change The valve will remain in its current position A The valve will allow flow to the left Closed The valve will close B The valve will open flow to the right The valve states are shown in Figure 17 CON LN D Vee Figure 17 Automated Valve Positions Looking Down at Valve A box to the right of the state buttons will be checked to show the valve s current state If Update Continuously is selected the valve will move immediately when you select a new position and the green button to the left of that position will be marked When Update Continuously is not selected choose the new position its green indicator will be marked then click Apply Settings Now to move the valve the check mark for the new position will be marked Current Valve State These features are only applicable when the Monitor box on the Control Panel is checked The valve position will remain Indeterminate until the valve direction is set or changed Once the position has been set the current valve state will be indicated by a check mark next to the correct position 22 CAUTION The software does not detect whether a valve is actually installed in each position in a manifold If the valve position is changed with no valve installed and then a valve is installed the position indicator could be incorrect
36. nt Syringe 21 ul 0 0 0 186 1130 Reagent Manifold Valve 1 Valve 2 valve 3 Valve 4 Open Open Open Open C close C close C close C close E Open B C Open B C Open B C Open B Reagent Syringe missing but used in script volume l Flow rate target ul ulmin J 20 0 ul 0 186 Output 22 36 40 771 Parsing script 22 36 40 771 Error on line 6 Unexpected characters found Syringe 22 36 40 771 Error on line 17 Label not found Fill_Syringe 22 36 40 771 Parsing failed with 2 errors in 17 lines 22 36 40 771 1 uDevice declared in the script has not been found 22 36 40 771 ot cannot be run until the script errors are resolved and the declared uDevices are found Ez uDevices fe Sequencer 4 4 gt bl Script Parsing f Sequence Status A Communications Sequence idle l 20 0 TAP NUM SC Figure 24 Error Message Referencing a missing device For this example the script references Reagent Syringe but the syringe is named Syringe 1 This error can be resolved by renaming the device see Device Naming and Numbering or changing the script To change the scrip Right click on Reagent Syringe under the Missing devices tab and select Rename Change the name to Syringe 1 and click OK Figure 25 shows how all instances of Reagent Syringe in the script have now been changed to Syringe 1 32 or uProcess MERE File Edit View Ac
37. o not soak or 48 submerse the entire syringe in any cleaning agent Prolonged contact with cleaning agents may damage bonded parts Sterilizing Syringes may be sterilized with appropriate sterilizing agents such as ethylene oxide Autoclaving Syringes can be autoclaved up to a temperature of 115 C However please be aware that over time autoclaving will shorten the syringes life expectancy Avoid rapid temperature changes which can lead to splitting of the syringe s glass barrel Stainless steel expands faster than glass upon heating so for most terminations autoclaving strains glued surfaces and may eventually lead to adhesive deterioration and leakage The plunger should be removed from the syringe before autoclaving Disinfecting If your application only requires disinfection we recommend the use of Microcide SQ Hamilton Part No 3995 01 This disinfectant is rated to eliminate the majority of commonly encountered bacteria viruses fungus and mildew Use of other common chemicals like 10 bleach acetone or ethanol are acceptable but are not rated to be as effective as Microcide SQ Capillary and Tubing Installation Guide LabSmith s CapTite one piece fittings enable connection of components valves syringe pumps interconnects bonded port connectors Luer Locks and cartridges to 360um capillary 1 32 tubing or 1 16 tubing To install the capillary or tubing 1 Cut the capillary or tubing to lengt
38. olding the wide end of the plunger installation wrench insert the wrench around the plunger tip and into the syringe until it engages with the hex head at the base of the syringe plunger Figure 40 Figure 40 Plunger Installation Wrench inserted into syringe CAUTION Be very careful not to damage Teflon end of the plunger Damage to the plunger can cause the syringe to leak 9 Turn the wrench counterclockwise approximately two revolutions to unscrew the plunger 10 Gently pull the wrench and plunger out of the syringe 46 CAUTION Do not operate the syringe without a plunger installed Doing so can damage the syringe To install the syringe glass and plunger 1 While holding the wide end of the plunger installation wrench insert the plunger assembly into the wrench such that the hexagonal feature on the plunger is engaged with the wrench 2 Gently insert the plunger assembly into the syringe and screw onto the shaft until hand tight approximately two revolutions 3 Carefully press the tapered glass syringe over the plunger assembly and into the syringe until it is seated in the syringe body 4 Use the hex wrench to tighten the screw located on the top side of the syringe Gently pull on the syringe glass to ensure it is secure in the syringe body The glass size value must be input in the software each time you change the glass size to allow uProcess to display and compute correct volumes and volume flow rates To s
39. owing the current valve position s will remain checked and the box showing the desired position will be shown as a solid green square Figure 19 When you click Apply Settings Now the valve s will change state and the new valve positions will be checked valve 1 valve 1 Valve 1 F Open _ Open F Open Close _ m Close J Close Open B Open B _ Open E Current State Click Close Click Apply Settings Now Figure 19 Changing a Valve s State in the Device Overview Window The indicator lights on the valve will illuminate when it is being actuated A red light indicates that it is being actuated to the A position while a green light indicates it is being actuated to the B position The indicator light on the 4VM manifold is green when the position of at least one valve is indeterminate The light will momentarily turn red whenever a valve has reached its open position 24 CHAPTER 5 AUTOMATED CONTROL An automated script is a series of steps for controlling one or more automated devices Each step can change the state of multiple devices A script can be created using the Process Wizard and it can be created or edited using the built in text editor Opening Script Files Each automated script is stored as a file with a usq extension To open an existing script click the Open A toolbar button then browse for the correct file Its contents will appear in the Main Window Figure 20 Fea
40. ript cannot be run 2 The Script Parsing tab of the Output window will display the message The script cannot be run until the script errors are resolved 3 The Script Parsing tab of the Output window will also list the line number and specific error 4 The step with the error will show a broken icon in the Sequencing Script list 30 In the example shown above the errors are in lines 6 and 17 The step name in line 6 Fill Syringe contains spaces which are not allowed The Loop command in line 17 is pointing to a step named Fill Syringe which cannot be found If Fill Syringe in line 6 is replaced with Fill Syringe the errors will be resolved and the script will be ready to run gt uProcess File Edit View Actions Help c bel a mG Sequencer 2 Sequencing Script PE heagent Syringe 5P501 20 ul Reagent Manifold 4 M01 Open Reservoir Reagent Manifold Setvalves 1000 Ec WaitAllbone gt Fill Syringe Reagent Syringe SetFlowRate 50 000 ulfmin Reagent Syringe MoveTo 10 000 ul Fc WaitAllbone gt i Open Process Reagent Manifold Setvalves 3000 F lt Pause gt 1000 ms Deliver Reagent Reagent Syringe SetFlowRate 10 000 ulmin Reagent Syringe MoveTo 0 000 ul E lt WaitAllDonie lt Loop gt Fill Syringe 10 ee tt a H E i a i E rh BB m TEEPEE E3 uDevices i Sequencer Sequence idle Ready aa wax keagent Syringe keagent Manifold Open
41. s and the step progression can be monitored real time As shown in the figure below the Device Control Overview Window shows the real time positions of the syringes and valves the Sequence Status tab of the Output Window shows which step is currently running and the number of iterations which have been completed The bottom left corner of the window shows how long the script has been running Figure 33 37 P uProcess File Edit View Actions Help EOF bp i Or wa Ol ae uDevices wx B Fl Interfaces iw EIB on COMI Reagent Syringe Addr 07 Reagent Syringe Reagent Manifold Addr 12 E AS Parata target 0 000 ul 8 341 oul 10 000 ulmin ja N y o S 21 ul 0 0 0 186 1130 Reagent Manifold Yalve 1 valve 2 Yalve 3 valve 4 fas C Open C Open C Open open C close C close C close C close Open B C Open B F Open B F Open B Output 23 02 45 583 Script Running Fill Syringe iteration 2 23 02 45 849 Script Running Open_Process iteration 2 23 02 46 865 Script Running Deliver_Reagent iteration 2 23 02 53 849 Script Running Fill Syringe iteration 3 23 02 57 146 Script Running Open Process iteration 3 23 02 58 146 Script Running i iterati Fa uDevices fa Sequencer M 4 gt gt Script Parsing Sequence Status 4 Communications Deliver_Reagent iter 00 00 53 141 Ready NUM Figure 33 Sequence Status Tab Syringe Pump Volume vs Delivery Time In the above ex
42. step to the script and go on to the next step 2 Sh ee Set the Target Volume for the syringe named Reagent Syringe to 10 ul and the Flow Rate to 50 ul min Figure 28 34 P uProcess File Edit View Actions Help BREA MEER e SEC b ii a QO 2l uDevices wx G El Interfaces Hg S EIB on COMI Reagent Syringe Addr 07 4 Reagent Manifold Addr 12 Reagent Syringe Volume Flow rate A target 10 000 ul 0 005 ul 50 000 ul min ja J E S cece 21 ul 0 0 0 186 1130 Reagent Manifold valve 1 Yalve 2 Valve 3 Valve 4 Open Open Open Open o C close C close C close C close OpenB lopen 6 OpenB OpenB Output 22 09 19 271 Parsing script 22 09 19 271 Parsing succeeded with no errors in 9 lines 22 09 19 271 All ubevices declared in the script are connected 25 uDevices fat Sequencer M 4 gt gt Script Parsing 4 Sequence Status A Communications Sequence idle Figure 28 Step Fill Syringe Settings Fr 9 Depress the Do Not Change uDevice Settings button next to the Reagent Valve 10 Click the Save As a New Sequence Step button 11 Name the new step Fill Syringe 12 Choose wait until all devices are finished and Continue Figure 29 Creating a new sequence step Mew step name Fill_Syringe After changing the ubevice settings O Fause 4000 ms Wait until all devices are Finished O
43. t File O O O NN ODDO VW WI ma a O O 10 11 11 11 12 13 13 14 15 16 16 17 17 19 19 19 19 20 20 21 21 21 22 23 24 24 25 25 25 26 Run Pause and Quit a Script Automated Script Functions General Formatting Rules Edit Automated Script Text Other Sequence Script Functions Script Errors Missing Devices Example Script Syringe Pump Volume vs Delivery Time APPENDIX A MENUS TOOLBARS WINDOW CONTROLS Menu Bar Toolbar Tooltips Customizing the Dashboard Show Hide Dashboard Elements Auto Minimize Windows Detach Dock and Re Position Windows APPENDIX B MAINTENANCE Updating Firmware Valve Actuation Profile Settings Changing the Syringe Pump Glass Assembly Calibrating the Syringe Pump Care of the Syringe Glass and Plunger Plunger Care Cleaning Sterilizing Autoclaving and Disinfecting Capillary and Tubing Installation Guide 26 27 29 29 30 30 31 33 38 41 41 41 42 42 42 42 42 45 45 45 45 47 48 48 48 49 CHAPTER 1 INTRODUCTION LabSmith s uProcess is a platform comprised of a suite of hardware and software products that simplify construction and control of microfluidic systems uProcess automated devices work together with CapTite microfluidic interconnect products to make it easy to build and rebuild micro and nano fluidic circuits All products are inter compatible and quick to assemble taking the hassle out of fluid routing SPS01 Programmab
44. th uDevices To add a breadboard connect the 20 pin communication cable from the Device Bus port on the EIB to the female connector on the breadboard Line up the tab on the communication cable with the notch on the connector Installing Automated Valves To install and configure AV201 automated valves Plug the VM4 valve manifold into one of the connectors located along the side of the breadboard Secure the automated valve s to the desired location on the breadboard using two breadboard mounting screws Use a ribbon connector to connect the automated valve to a port on the valve manifold Observe the label on the valve and manifold to ensure the cable is connected at the correct orientation Installing Syringe Pumps To install an SPSO1 programmable syringe pump plug the syringe pump body into one of the connectors located along the side of the breadboard Two M3 screws may optionally be used to more securely attach the syringe to the bottom of the breadboard Installing uProcess Software uProcess software runs on any PC compatible computer running Microsoft Windows XP NT or 7 The software requires approx 5MB of free disk space To install uProcess 1 Insert the uProcess for Windows CD 2 Double click SETUP EXE and follow the on screen instructions 3 Torun the software double click the uProcess icon on your desktop or choose Run gt uProcess gt uProcess 10 CHAPTER 3 UPROCESS SOFTWARE Software Interface
45. tions Help 1 cF bell l Amol gt uDevices Syringe 1 SPSO1l 20 ul Feagent Manifold AVH Open Reservoir Reagent Manifold SetValve s 1 0 0 O E Interfaces WaitallDone gt Sle EIB on COMI Fill Syringe a Syringe 1 Addr 07 A ie ee ee eae ulmin yringe 1 oveTo u a Reagent Manifold 4ddr 12 TaitillDone gt Open Process Reagent Manifold SetValves 3 0 0 O Fause 1000 ms Deliver Reagent Syringe 1 SetFlowRate 10 000 ulmin Syringe 1 MoveTo 0 000 ul lt WaitAllDone gt Loop Fill Syringe 10 Output 22 41 12 450 Parsing script 22 41 12 490 Parsing succeeded with no errors in 17 lines 22 41 12 490 All udevices declared in the script are connected 2241 12490 The script is ready to run F r ee Te TET M 1 1 Ee uDevices fat Sequencer H o4 H Script Parsing Sequence Status Sequence idle NUK Figure 25 All instances of Reagent Syringe have been changed to Syringe 1 in this script Example Script In the example shown in Figure 26 a syringe pump is attached to an automated valve a supply reservoir and a downstream process The syringe will first pull fluid from a reservoir connected to the A port of the valve The valve will then switch to the B position and the syringe will deliver the fluid to the downstream process Syringe Pump Downstream Process Figure 26 Hardware Setup for the Sample Process For this example the following devices are installed on one EIB
46. ur Valve Driver amp Interfaces P Reagent Syringe Addr 02 alve M told A t Valve Manifolc chy Dock at Top ae amp EIB on coms faton Fonie Monitor P Semple Syringe Addr 16 Valve 1 Valve 4 No change OA Dock at Right Side gt lt q Dock at Bottom 43 44 APPENDIX B MAINTENANCE Updating Firmware The firmware for all uProcess devices can be field updated as follows 1 Download the latest version of uProcess software from www labsmith com 2 Choose Actions gt Firmware gt Update All Firmware uProcess will upload the latest firmware associated with the installed software to all connected syringe pumps and 4VM manifolds Devices that are not attached will not be updated Note You can update the firmware for a particular device by clicking the Configuration button in the Device Control Panel Valve Actuation Profile Settings uProcess devices are factory configured for optimal performance From time to time it may be necessary to access and adjust these settings To do so from the Device Control Panel click the Configuration button which will open one of the Configure Device dialog boxes shown in Figure 37 Note Contact LabSmith before changing any of the Actuation Profile Settings Configure 4VM Mame Device Address Actuation profile settings Initial knob 100 Stall holdoff 3 Start Current 96 Duration frist SO Cruise Current 19
47. uration a am v ine v itor LabSmith 5P501 Addr 27 Saha ee LabSmith P501 Addr 53 Se EIB on COMI Speed Control 8 4 Valve Manifold Addr 01 ul min LabSmith SPS01 Addr 108 0 46 a i Bt me 2800 Position Monitoring amp Control Gy Set Point a sees aE TEN 54 ul 0 0 ul oO 23 uDevices A Sequencer Output 12 44 33 921 Communications port 11 closed 12 44 51 312 Communications port 11 opened 12 49 03 609 Communications port 1 closed Communications p p A Script Parsing A Sequence Status Communications A Sequence idle Ready Figure 8 Control Panel for Syringe Pump P uProcess File Edit View Actions Help Dea EE SET eee uDevices 4Valve Manifold P g a Interfaces Four Valve Driver Properties S EIB on COM11 Address 1 abSmith SPS01 Addr 27 Online Monitor LabSmith SP501 Addr 53 Se EIB on COMI B 4 Valve Manifold Addr 01 valve 1 Valve 2 valve 3 valve 4 LabSmith 5P501 Addr 110 No change No change No change No change Oa Oa Oa Oa O closed O closed O closed O closed OB OB OB Os Indeterminate Indeterminate Indeterminate Indeterminate 23 uDevices A Sequencer Output 12 49 03 609 Communications port 1 closed Communications port 1 opened Communications port 11 Error Failed to read from port Disconnecting Click Online to try to reconnect Communications port 11 close ia Script Parsing A Sequence Status Communications A
48. ve Step Name Step names may have a maximum of 16 characters and may not include blanks or gt or 7 Select how the script should respond after changing the uDevice settings a Select Pause then enter the duration to pause before continuing with the process The pause duration begins as soon as the step starts b If you select Wait until all devices are finished uProcess will wait until all devices have completed their functions before continuing on with the process c Select Do Not Wait to immediately continue on to the next step 8 Select how the script should continue after this step a Select Continue to continue to the next step in the process If a new step is not added the process will end b Select Loop to step to jump to another process step Select the target step from the drop down list then enter the number of times the step should loop c Click OK The step will appear in the Sequencer Window and its text will appear in the Main Window Save a Script File Click the Save toolbar button or choose File gt Save to save all changes to the current script file If the file has not yet been saved you will be prompted to provide a name and file location for it Choose File gt Save As to store the current script under a new file name Run Pause and Quit a Script When a script is open and error free the Run am toolbar button will be available Click Run to initiate the process
49. vices Window click on the icon for a syringe pump to view its Control Panel Figure 12 19 Reagent syringe S Pump Properties Address 2 Syringe Size Standard 20 ul Online Monitor Speed Control ulimin 0 186 1130 Position Monitoring amp Contral u 4 10 000 ul 71 ul 0 0 ul 4478 a Sek Point ul Figure 12 Controls for an SPS01 Programmable Syringe Pump In addition to the Common Controls described earlier you can view perform the following Current Syringe Pump State The following features are only applicable when the Monitor box on the Control Panel is checked The diagram at the bottom of the window shows the current pump state Figure 13 Max Volume Current Volume 21 ul EN e pase wa Motion Indicator r Stall Indicator Figure 13 Syringe Pump Diagram The Current Volume of the syringe is indicated by the colored portion of the syringe diagram When the plunger is stationary the fluid volume is shown in blue When the plunger is pulling loading the color will change between blue and red and when it is pushing dispensing the color will change between blue and green The Motion Indicator shows the direction the syringe plunger is moving Red syringe plunger is pulling loading Green syringe plunger pushing dispensing Grey syringe plunger is not moving The red Stall Indicator will appear if the syringe pump has stalled It will sta
50. y on until you click the Stop the uDevice x or Stop All uDevices JO button Position Control The position of the syringe plunger can be set using one of several methods Figure 14 20 Move in Increments Max 10 1 0 1 of Range Move in Move to Increments of Set Point KJ ju E i J Set Point 10 000 ul Actual D Move to Set Point Figure 14 Set Syringe Position 1 Move to a set point by clicking and dragging the Set Point slider bar to the desired volume Move to a set point by typing a volume into the field to the right of the Set Point slider Enter an Increment into the field above the Set Point slider then click the arrows on either side of the box to increase decrease the set volume Click the lt and gt buttons to move to the maximum extents of the plunger travel Click lt lt lt lt lt or lt to increase the volume by 10 1 or 0 1 of the maximum volume Similarly click gt gt gt gt gt or gt to decrease the volume by 10 1 or 0 1 In all cases the plunger will move to the new volume at the speed set in the Speed Control Speed Control To set the flow rate speed of the pump click and drag the Speed Control to the desired flow rate or type the flow rate into the ul min box Figure 15 The minimum and maximum flow rates for the syringe pump are shown below the slider bar Speed Control 100 ulfmin 0 186 1130 Figure 15 Speed Control Settings Optim
51. you click Apply Settings Now Click the button again to allow setting changes to be made 23 Click the Stop This uDevice button to stop the action of the particular device and leave all other devices unaffected Syringe Pump Settings These controls are similar to those on the syringe pump controls screen Click and drag the Target Volume slider or enter the desired volume in the Target box Click and drag the Flow Rate slider or enter the desired rate in the Flow Rate box If Apply Settings Continuously is selected then the pump will move immediately to the new target volume at the selected flow rate If Apply Settings Continuously is not selected the pump will remain at its current settings until you click Apply Settings Now The indicator light on the syringe pump will flash red green when it is first powered up It will glow green when the syringe is pushing and red when it Is pulling Automated Valve Settings The Automated Valve controls are similar to those on the Valves Control Panel For each 4VM manifold four valves are shown regardless of the number of valves actually attached For each valve you wish to change click the box next to Open A Closed or Open B these states are described in Valves Control Panel lf Apply Settings Continuously is selected then each valve will change position immediately and the box next to the new state will be checked If Apply Settings Continuously is not selected then the box sh

uProcess User Manual v1.0

Contents

Download Pdf Manuals

Related Search

Related Contents