Home

Typhoon™ FLA 7000

1. gt gt CAUTION Always wear cotton gloves when handling storage phosphor screens Laser safety WARNING Never detach the inner cover screwed to this instrument If it is detached laser beam may leak with a risk of loss of vision gt gt CAUTION Using procedures or adjustments other than those specified in this manual may result in hazardous exposure to laser radiation Radiation safety This instrument is not equipped with any radioisotope RI or radiation generating unit and is therefore not regulated by radiation hazard prevention laws However the 16 Radiation safety instrument is capable of reading Storage phosphor screens which may be polluted by radioisotopes RI A CAUTION If radioisotope RI pollution occurs stop use of the instrument immediately and follow the instructions of your radiation administrator Typhoon FLA 7000 User Manual 28 9607 62 AC 2 Safety instructions 2 6 Radiation hazard prevention 2 6 Radiation hazard prevention Controlled area Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation Hazards due to Radioisotope and so forth Prime Minister s Office ordinance No 56 defines the controlled area as a place where the dose equivalent related to external radiation exceeds the dose equivalent determined by the Director General of the Science and Technology Agency hereinafter referred to as the
2. ETET e Ee eere EREE 3 Confirm the uninstallation by clicking Yes when prompted 4 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Uninstalling Typhoon FLA 7000 Control Software under Windows 7 Step Action l Open the control panel and select Uninstall a program under Programs 54 Typhoon FLA 7000 User Manual 28 9607 62 AC Step 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software Action Select Control Software and then click Uninstall Confirm the uninstallation by clicking Yes when prompted If User Account Control UAC is enabled in Windows 7 a dialog displays the message An unidentified program wants to access your computer Click Allow Upgrading Typhoon Control Software Step FLA 7000 Action 1 2 Uninstall the current version of Typhoon FLA 7000 Control Software Install the new version of Typhoon FLA 7000 Control Software Follow the instructions in the installation chapter of Getting Started Typhoon FLA 7000 User Manual 28 9607 62 AC 55 7 Registering editing and deleting methods 7 7 1 56 Registering editing and deleting methods Note Method settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to change these settings Registering a new method Step Action
3. 9 8 Scanning the sample Click the Start Scan button to start reading the sample The scanning progress window opens 74 Typhoon FLA 7000 User Manual 28 9607 62 AC 9 Operation 9 8 Scanning the sample Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You cannot start reading again from the location where reading stopped 9 9 Adjusting the display parameters Step Action 1 Adjust the display parameters if desired Follow the instructions in Section 10 2 Display parameters explained on page 81 2 Click Return to return to the Reader Settings screen Typhoon FLA 7000 User Manual 28 9607 62 AC 75 9 Operation 9 10 Saving the image data using a different file name 9 10 Saving the image data using a different file name Step Action 1 After scanning the sample click the Save as button The following dialog opens co rx Swen Distro hat SER 2 Type a file name in the File name field 3 Select a file format in the Save as type drop down list 4 Save the image file by clicking the Save button 9 11 Viewing the image file in the analysis application Click the Launch button in the to open the image file in the registered analysis application Note If no analysis application is registered in Typhoon FLA 7
4. e Click the Change button to install to a different folder Click the Install button If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Click Install this driver software anyway in the Windows Security dialog Windows Security Click the Finish button The installation of Typhoon FLA 7000 Control Software is now completed 44 Typhoon FLA 7000 User Manual 28 9607 62 AC 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 5 4 Install Typhoon FLA 7000 Control Software Windows 7 Before you begin Log in using a Windows account with administrator privileges Disable the computer sleep function In order to prevent scanning errors the computer should be set to never sleep as described below 1 Open the Control Panel and click System and Security Click Change when the computer sleeps below Power Options 2 Select Never in the drop down menu by Put the computer to sleep then click Save changes Typhoon FLA 7000 User Manual 28 9607 62 AC 45 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 Installation of the USB Control Driver Windows 7 Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permissio
5. Noise 70 dB A or lower Degrees of protection provided by enclosure IP20 Typhoon FLA 7000 User Manual 28 9607 62 AC 101 For local office contact info rmation visit GE imagination at work and GE monogram are trademarks of General Electric Company www gelifescie nces com contact Typhoon is a trademark of GE Healthcare companies GE Healthcare Bio Sciences AB All third party trademarks are the property of their respective owners REN 2010 General Electric Company All rights reserved Bj rkgata n 30 First published Oct 2009 75184 U ppsala All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information Sweden www gelifesciences com quantitative_imaging GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan imagination at work 28 9607 62 AC 07 2010
6. 1 Click the Method button in the main window The Method Settings dialog box appears Method Settings Method Settings 532m Ej635nm Eg 635nm 532m E 532m 473nm 9473nm Gi650nm E 532m Typhoon FLA 7000 User Manual 28 9607 62 AC 7 Registering editing and deleting methods 7 1 Registering a new method Step Action 2 Click the Add button The following dialog box appears Method Name EAE Laser E8 532nm Filter rv520 2K cance 3 Select a laser in the Laser drop down box You can select lasers that are not currently loaded 4 Select a filter in the Filter drop down box You can select filters that are not currently loaded 5 Type a name in the Name field 6 Click the OK button The list in the Method Settings window now contains the new method with the selected laser and filter combination Note Methods containing lasers or filters that are not currently loaded cannot be selected in the Reader Condition screen Typhoon FLA 7000 User Manual 28 9607 62 AC 57 7 Registering editing and deleting methods 7 2 Editing a method 12 ro 58 Editing a method Step Action 1 Click the Method button in the main window The Method Settings dialog box appears Method Settings E p Method Settings 532m Cy5 Ege35nm R670 DDAO Ege35nm R670 Deep Purple E 532nm 0s580 EtBr Eg532nm 0580 FAM 3473nm v520 FITC Einm rs200 Phos
7. 100 um 150 s 50 um 210 s 25 um 330 S File Format Determines the file format used to save the reading data Gel Image File gel The standard file format is a gel file which contains square root encoded pixel data Gel Image File gel Tiff Image File tif In combination with a gel file a read image can also be saved in TIFF file format For TIFF files image data type is always set to Linear format Launch Appli cation Contains the path to the application used to analyze the data Select the analysis application by clicking the Select button Then browse to the software executable and click OK Typhoon FLA 7000 User Manual 28 9607 62 AC 83 10 Reading conditions display parameters and other settings 10 3 Other settings User account specific settings Changes to the settings in the Preferences dialog affect only the current Windows user account Changes made using a different user account do not affect the current user account 84 Typhoon FLA 7000 User Manual 28 9607 62 AC 11 About storage phosphor screens 11 About storage phosphor screens 11 1 Introduction The Storage phosphor screen is a radiation energy memory type two dimensional sensor which has an image recording layer consisting of polyester base material densely coated with accelerated phosphorescent fluorescent material of fine crystals e Exposure A Storage phosphor screen accumulates and stores radiation energy
8. CAUTION Activate the Typhoon FLA 7000 at least once every 30 days to execute calibration This calibration ensure that the SHG laser operates properly Failure to calibrate the Typhoon FLA 7000 every 30 days reduces the lifespan of the SHG laser Operation procedures 1 Turn on the Typhoon FLA 7000 and the computer 2 Wait until warm up and self diagnosis is completed and the scanner is ready Only the power lamp on the upper left panel is lit The automatic calibration has now been performed 3 Turn off the Typhoon FLA 7000 and the computer 90 Typhoon FLA 7000 User Manual 28 9607 62 AC 12 Maintenance Cleaning the stage CAUTION Wear gloves to prevent direct contact with chemical substances Step Action 1 Remove the stage from the main body of the instrument 2 Wipe the stage with a sponge moistened with a fluorescence free neutral detergent 3 Thoroughly rinse the stage with water and dry with a lint free cloth 4 Place the stage in the main body of the instrument Storing the Fluor or Multi stage Store the Fluor or Multi stage in the original packaging when they are not in use DO NOT store stages inside Typhoon FLA 7000 Typhoon FLA 7000 User Manual 28 9607 62 AC 91 13 Troubleshooting 13 Troubleshooting This chapter describes various problems that can foreseeably occur with the Typhoon FLA 7000 Control Software Suggestions of possible countermeasures are given Do the
9. 2 TT Fehm TTT el 7 3 Reader Settings Fluorescence ee oad Pixel Size Latitude 4 tees fia 7 SS ir 8 e ii eso pm wut so 800 1608 5 14 Ete j Part Description 1 Image folder specify where to save the file after the reading 2 File Name enter the name of a file to save image data 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software 4 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods 5 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity ji to increase the number of scans to decrease the number of scans Typhoon FLA 7000 User Manual 28 9607 62 AC 1 Introduction 1 4 Typhoon FLA 7000 Control Software Part Description 6 Set the scanning area 1 Select a stage in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Latitude Specify the dynamic range e L5 provides a larger detectable range than L4 e L4provides a finer density gra
10. 27 35 ACCESSO ES nahua a a a a aa e i aR 30 4 Installing and moving Typhoon FLA 7000 scssssssssssssessssesssssereressereres 34 AA SIRE CLITA SES eian oqo AERA I RLE ER 34 4 2 Transporting Typhoon FLA 7000 woeceecscccsssssssssssesssssccsssssssssssssssessessecssssssssusuesssesscsssssssssussssssseceees 35 4 3 CONNECtHIOnNS sssisssissistesscsssnassoaschssnsscesssedassenussccasstassabcodsseddudsodzascassnsbsaasedidedissosstssoaastssonateadasddeassssssccasastons 35 5 Installing Typhoon FLA 7000 Control Software sssssssessssessesssessessssessesesssses 37 5 1 Installation Sequence o cssessssesssssssssssssssssssssssssssssscsssssssssussssesscssssssssisussssesscessssssssussuecsssscesssssssssassees 37 5 2 Install Typhoon FLA 7000 Control Software Windows XP ssssesssesssscccsssssssseesesssssecsssesens 37 5 3 Install Typhoon FLA 7000 Control Software Windows Vista 41 5 4 Install Typhoon FLA 7000 Control Software WiINGOWS 7 ssssssessesssssccsssssssseeseessessesssssess 45 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software 53 7 Registering editing and deleting methods 56 7 1 Registering a MEW method wees 56 Le Editing ameti desarsinar Raa 58 La D letingamethod sssresimneniiernninann anna a a 58 8 Filter mod le Settings aise ances cesek sachs se cccadeciadeiccbad ecco bed etter kaiaa E 60 8 1 Registering a new filter NAME esssesssessssssscsssssssssssssesssessecssssssssssssssessecssssssssussssse
11. 8 Filter module settings 8 4 Installing and replacing filters Place the filter module in the scanner Step Action 1 Open the filter change door 3 Close the filter change door Register the filter change in Typhoon FLA 7000 Control Software Step Action 1 Click the Filter Module button in the main window U Filter Module 64 Typhoon FLA 7000 User Manual 28 9607 62 AC Step 8 Filter module settings 8 4 Installing and replacing filters Action Click the desired filter position on the filter tray Filter Module Module g IP 2 ij 520 g h Load Filter Module Select the filter to be registered from Filter List Filter Module Settings Filter Module Modulet 1 0 o 2 fi s20 3 fi Reo Load filter Module 4 p 0800 Cancel a r Click the Insert button to register the filter gt Insert Tip You can also drag the filter from Filter List to the desired filter position Click the OK button Typhoon FLA 7000 User Manual 28 9607 62 AC 65 9 Operation eel 2 i Operation Operation overview Using Typhoon FLA 7000 comprises a series of steps outlined below Detailed explanations are provided in subsequent chapters Turn on the Typhoon FLA 7000 Phosphorimaging samples Start the computer and launch the Typhoon FLA 7000 Control Software Expose the storage phosphor screen Set the reading parameters Place the stage
12. AC 3 System configuration 3 3 Accessories Fluor stage included The Fluor stage is used to read gels or membranes e Place the gel or membrane directly on the glass of the Fluor stage e Place the Fluor stage in the instrument Glass surface Membrane weight included The membrane weight is used to hold thin fluorescent samples flat against the Fluor stage gt gt Multi stage included The Multi stage is used to read gels with glass gel merely supported by glass or titer plates e Place the gel with the glass plate directly on the Multi stage or place a titer plate on the titer plate plugin attached to the instrument e Place the titer plate on the Multi stage Typhoon FLA 7000 User Manual 28 9607 62 AC 31 3 System configuration 3 3 Accessories Phosphor stage included The phosphor stage is used when reading storage phosphor screens The stage is magnetic to hold storage phosphor screens e Place the phosphor stage upside down on a flat surface e Place the storage phosphor screen on the phosphor stage with the reading surface facing away from the phosphor stage e Place the protective cover on top of the storage phosphor screen e Flip the phosphor stage over e Place the phosphor stage in Typhoon FLA 7000 Storage phosphor screen sticking side Power cord included Typhoon FLA 7000 uses a special AC power cable Be sure to use power cables specified in service manuals o
13. Director General the concentration of radioisotope in the air exceeds the concentration determined by the Director General or the radioisotope density on the surface polluted by radioisotope exceeds the density determined by the Director General Limit of superficial pollution Paragraph 3 of Article 4 of Notice No 15 of the Science and Technology Agency that determines the quantity etc of radiating isotope specifies that the density of radioisotope onthe surface polluted by radioisotope must be one tenth of the density defined in Article 8 Article 8 and Table 3 of this Notice define the limits as shown below 1 Superficial density of radioisotope that radiates alpha rays 4 Ba cm 2 Superficial density of radioisotope that does not radiate alpha rays 40 Bq cm Installation site of instrument This instrument is capable of reading not only Storage phosphor screens but also fluorescent pigment label samples non RI method Therefore it is recommended that the user should install it outside the controlled area and use RI indicated samples without contacting them with Storage phosphor screens directly However as described above the Storage phosphor screen surface may be polluted by radioisotope RI depending on the sample condition since the instrument sticks the sample to the 3H compatible Storage phosphor screen surface and exposes it in an auto radiography experiment of the 3H label sample When a sample is in direct contact
14. KD RIMES CSMOFIRCHCED TSE 2QITWAIEVIA WeECYESSRt AREN E CHER ICFLA 7000R ANE LIAN CCK EEL CCRT 1YS Oy DEMIR 1 Please set or exchange filter as directed in operation DIAKRUTIRAAUSS manual CLOBIECERI TIER 2 Please insert the filter module into FLA 7000 firmly CAUTION till it reaches the end Sa LENS JEFEATED AVOID EXPOSURE TO THE BEA ATTENTION RAYONNEMENT LASER VISIBLE DE CLASSE 3B A LOUVERTURE LE VERROUILLAGE EST DEJOUE EVITER L EXPOSITION AU RAYO Te NAF UVSBAOE VITBRICELAGHESICVY FUT lt KKS REATF VICLY b CSSUVUIVOEASOmMMUFCS 1 Please insert the stage so that it fits pins on the loading sectio 2 Sample thickness on the fluor stage is 30mm or less Typhoon FLA 7000 User Manual 28 9607 62 AC 21 22 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 Labels at rear of Typhoon FLA 7000 IFIMAGFAR IMAGE ANALYZER MOREL Tuphopp UA TIg0 SERIAL No MANUFACTURED Tha device compiles with part 15 of the FOC Rules Operation a subject to the folowing tine conditions 1 the device may not cause hard rtecterence and 2 tha device must accept any merierence received nchxing interference that may Cause undeseed operation The Cass A dgtal apparatus meets al requrements of the Canadian iterference Caunung Equpment Reparera Cot apparel remenque de la classe A respecte toutes tes exigences du Reviernentt sur ke matari boulieur dy Canada ze So
15. OMKEISAIB OCRKDU TURES F EDOM CERI TCR Typhoon FLA 7000 User Manual 28 9607 62 AC 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 Labels on left side of Typhoon FLA 7000 Power ON O Power OFF EEn Ta TOEMKLIS23 B OURAROPTRAD WES E LOMIE lt IF TCE CAUTION CLASS 38 VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM ATTENTION RAYONNEMENT LASER VISIBLE ET INVISIBLE DE CLASSE 3B A LOUVERTURE EVITER L EXPOSITION AU RAYON 2 9 Emergency procedures This section describes how to do an emergency shutdown of the Typhoon FLA 7000 instrument The section also describes the results of a power failure Emergency shutdown Step Action 1 Click the Stop button in the Typhoon FLA 7000 Control Software 2 Turn off the Typhoon FLA 7000 by pressing the power switch on the left side of the instrument body 3 Disconnect the power cord from the power outlet Typhoon FLA 7000 User Manual 28 9607 62 AC 23 2 Safety instructions 2 9 Emergency procedures Power failure The results of a power failure depends on the unit or units affected Unit affected by power failure Results Typhoon FLA 7000 instrument The reading is interrupted immediately The instrument is in an undefined state The data collected up to the time of the power failure is available in the file created when starting the scan Computer running the Typhoon FLA 7000 Control Software Th
16. Set The scanned image was overexposed due to a high PMT voltage setting Countermeasure Decrease the PMT voltage in the Reader Settings window door was opened Scanning stopped because the The door was opened during the scan Countermeasure Close the door and scan the image again Do not open the door until the scanning is finished Check the laser and filter The combination of the laser and filter might be inappropriate The selected method may be inappropriate Countermeasure Select another method or edit the method to suit the current application ware Sense Key 1H Error Code 2H 3H A scanner error was detected Please restart Typhoon FLA 7000 imager and Scanner Control Soft An error occurred during scanning Countermeasure Try to run the scan again Contact your GE Healthcare representative if the problem persists 96 Typhoon FLA 7000 User Manual 28 9607 62 AC 473 nm laser Appendix A Default sample detection methods Method name Filter type Cy 2 520 FITC Y520 SYBR Green Y520 SYPRO Ruby 0580 SYPRO Orange Y520 FAM Y520 Alexa Fluor 488 Y520 Digitize473 Y520 532 nm laser Method name Cy3 Filter type EtBR 0580 ROX 0580 Deep Purple 0580 Pro Q Diamond 0580 Digitize532 Typhoon FLA 7000 User Manual 28 9607 62 AC A Default sample detection method
17. and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numerique de la class A est conforme a la norme NMB 003 du Canada Typhoon FLA 7000 User Manual 28 9607 62 AC 1 Introduction 1 2 Regulatory information Note This is a class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Laser standards This instrument is a Class 1 IEC60825 1 2007 EN60825 1 2007 laser product E Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER MODEL Typhoon FLA 7000 SERIAL No MANUFACTURED FFTPT Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No 50 dated June 24 2007 405N101499 The following lasers can be installed i
18. button Typhoon FLA 7000 User Manual 28 9607 62 AC 37 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software Windows XP Step Action 6 8 10 11 38 Select Add a new hardware device and click the Next button a aa eutalied toy pe emmansiny rom te ht batom select an rate hae Gove tam chk Wed to come popene 3 a ptian pa a De NaS To wd harman ct home oh et chet AS a nee hana Srvc Select Install the hardware that manually select from a list Advanced and click the Next button Select Show All Devices and click the Next button From or bat baton select the Ippe of handiare pons ae witli 1 you do rot ea te han cacy pm mart cic howe A Dee Click the Have Disk button in the Add hardware wizard Insert the Typhoon FLA 7000 Control Software DVD and click the Browse button Select to install the driver from the Typhoon FLA 7000 Control Software DVD Lack a My Corpse 7 7 na G Oena Trehi C My Docuneris Sse F wy Congue Od Loca Dak IC Dan S STORE NGORI O Sheed Devaar Typhoon FLA 7000 User Manual 28 9607 62 AC Step 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software Windows XP Action 12 13 14 15 16 1 18 Open the USB Control folder Lock Typhoon FLA TOOD DUR function Select the DevMng inf file and click the Open button Lock L U58 Cornel Cli
19. instructions 2 Safety instructions 2 1 Introduction The Typhoon FLA 7000 is powered by mains voltage and is used to image samples that may be hazardous Before installing operating or maintaining the equipment you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment 2 2 General precautions WARNING Do not use the equipment if smoke strange noises or strange odors can be perceived or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE Healthcare representative to request repair WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Do not place the equipment on unstable tables or on inclined surfaces as the equipment could be dropped or fall resulting in injury Typhoon FLA 7000 User Manual 28 9607 62 AC 15 2 Safety instructions 2 3 Personal protection 2 2 4 2 5 Personal protection CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples
20. to detect tritium place the sample directly on the storage phosphor screen A tritium storage phosphor screen can be used only once Open the top cover of the cassette Unlock the cassette by sliding the buttons on both sides up Typhoon FLA 7000 User Manual 28 9607 62 AC 11 About storage phosphor screens 11 5 Exposing the storage phosphor screen Step Action 4 Place the sample on the cassette Make sure that the sample surface faces Note Keep the radio imaging sample away from the edge of the storage phosphor screen Otherwise the recorded data may become corrupted 5 Place the storage phosphor screen in the cassette with the exposure surface of the storage phosphor screen facing the sample Make sure that the notch of the storage phosphor screen is in the front left corner of the cassette as shown below 6 Close the cover of the cassette Press on the cover until it clicks into place Typhoon FLA 7000 User Manual 28 9607 62 AC 89 12 Maintenance 12 Maintenance Cleaning the outside of Typhoon FLA 7000 Clean the outside with a moist soft cloth and a mild detergent Wipe afterwards with a dry soft cloth Maintenance of the SHG laser If you use the SHG laser in the Typhoon FLA 7000 it will require periodical calibration When the Typhoon FLA 7000 is switched on it automatically executes calibration You do not need to do a manual calibration if the Typhoon FLA 7000 is used at least once a month
21. user information and a general description of Typhoon FLA 7000 and its intended use 1 1 Important user information Read this before using the Typhoon FLA 7000 All users must read this entire manual to fully understand the safe use of Typhoon FLA 7000 Intended use Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging ECL Plus Westerns visible fluorescence and gel documentation Typhoon FLA 7000 is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of Typhoon FLA 7000 See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Typhoon FLA 7000 User Manual 28 9607 62 AC 5 1 Introduction 1 1 Important user information Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A Note is used to indicate information th
22. while it is exposed It is exposed in close contact with an RI sample in a cassette like X ray film e Scanning The recording surface of an exposed Storage phosphor screen is scanned with a laser beam inside Typhoon FLA 7000 and emits fluorescent light according to the exposure level A photo multiplier tube PMT detects the fluorescent light and converts it into electric signals A radiation image recorded on the Storage phosphor screen during exposure is read as digital image information at the maximum resolution of 25 um per pixel 40 pixels mm and recorded in the analyzer unit e Erasure You may reuse a general purpose Storage phosphor screen by erasing an after image 11 2 Compatible storage phosphor screens Storage phosphor screens with magnetic layers for sticking on the phosphor stage are usable 11 3 Handling precautions Check the exposure environment NOTICE Avoid exposing storage phosphor screens in places where the environmental radiation may be increased for example rooms with concrete walls or in basements Do not stack cassettes during exposure Typhoon FLA 7000 User Manual 28 9607 62 AC 85 11 About storage phosphor screens 11 3 Handling precautions Wear gloves CAUTION Always wear cotton gloves when handling storage phosphor screens Use a suction rod when taking the storage phosphor screen out of the cassette Prying the storage phosphor out may result in the edge pe
23. with a Storage phosphor screen it is generally known that the sample for making an auto radiogram contains a relatively small quantity of radioisotope However the degree of superficial pollution of the Storage phosphor screen is greatly influenced by the dryness of the sample and dose of radioisotope in an experiment and may exceed the limits mentioned in Limit of superficial pollution on page 17 When the instrument reads a Storage phosphor screen with a polluted non exposure area it may be polluted The degree of such superficial pollution greatly differs with users operation conditions Superficial pollution may exceed the limit mentioned above Note As mentioned above install this instrument in the RI controlled area if the user uses Rl indicated samples that will be in direct contact with Storage phosphor screens Typhoon FLA 7000 User Manual 28 9607 62 AC 17 2 Safety instructions 2 6 Radiation hazard prevention Removal from the controlled area If it is necessary to move the instrument and its laboratory which were installed and have been used in the controlled area from the controlled area make sure that the degree of the superficial pollution is below the limits mentioned in Limit of superficial pollution above 2 7 Electric safety WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a si
24. 000 Control Software the Launch button is greyed out and inactive If desired register or change the analysis application 9 12 Turning off Typhoon FLA 7000 Step Action 1 Turn off Typhoon FLA 7000 by pressing the power switch on the left side of the instrument body 76 Typhoon FLA 7000 User Manual 28 9607 62 AC 10 Reading conditions display parameters and other settings 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Save condition Load condition Image n E File Name Image Folder Cogan FenGE Meare Typhoon FLA TODD Date File Name iru mateo Pixel size Comment a Sora ae Method M Qera co im ae Latitude von MQ 7608 c 200 pm A Sampling Area e C Stage PMT A BICIDIEIFIG Sampling c Mode Mode Grid Free O AAE il Size Reading Time about 1m45s LE z Reading Tir Top E Top RO Fer om O Start scan j Start Scan Image folder Specify where to save the file after the reading Note When reading more than once and reading of 1ch 2ch is implemented simultaneously a folder with a name set by File Name is created automatically at the specified position and the image data will be saved in the folder Typhoon FLA 7000 User Manual 28 9607 62 AC 77 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained File Name Enter the name of a file t
25. 000 User Manual 28 9607 62 AC 67 9 Operation 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software Start Typhoon FLA 7000 Control Software 9 4 68 Step Action 1 Make sure that Typhoon FLA 7000 has completed the warm up after which only the power lamp on the upper left panel on the front of the Typhoon FLA 7000 is lit Start Typhoon FLA 7000 Control Software from the Start menu or use the desktop shortcut The main window of the Typhoon FLA 7000 Control Software is displayed Fae Ee Help ONTROL SOFTWARE Mogeavon at work a re a pi The condition is displayed in the Status area of the main window Status messages are as follows Message Explanation Disconnected Cannot recognize Typhoon FLA 7000 Please check connection and power Warm up Typhoon FLA 7000 is in self diagnosis Please wait Ready The unit is ready to use Selecting the reading mode Click the button that corresponds to the desired reading mode Typhoon FLA 7000 User Manual 28 9607 62 AC 9 Operation 9 4 Selecting the reading mode To Click this button Read a fluorescent sample Read a phosphorimaging sample Digiizatio HiT Read a digitization sample 9 5 Setting the reading conditions Set the reading conditions by following the instructions in Chapter 10 Reading conditions display parameters and other settings on page 77 9 6 Placing
26. 29 x 283 x 35 Approx 2 Fluor stage 429 x 283 x 35 Approx 1 Multi stage 429 x 283 x 33 Approx 1 3 FLA Image Eraser 603 x 512 x 163 14 5 Note The Reading block is a standard unit The other units are optional Power supply Parameter Data Input voltage 100 to 240V AC Allowable variations in 10 voltage Frequency 50 to 60 Hz Rated current 15to3 0A 100 Typhoon FLA 7000 User Manual 28 9607 62 AC Environmental conditions B Specifications Parameter Data Operating conditions Temperature 15 to 30 C Humidity 30 to 70 no dew condensation Non operating condi tions Temperature 10 to 40 C Humidity 20 to 70 no dew condensation Transportation amp stor age conditions within 96 hours Temperature 25 to 70 C Humidity 10 to 80 no dew condensation Heat radiation 151 W h reader block FLA Image Eraser Lighting It is recommended to lower the lighting level to about 20 lux when moving a sample from the cassette onto the Phosphor stage after exposure 20 lux is the brightness level at which most people can read newspaper sized text after he she becomes accustomed to the brightness Location of use Indoor use only Maximum altitude for use 2 000 m above sea level Overvoltage category Transient overvoltage category II Applicable Pollution Rating Pollution Degree 2 Noise levels Parameter Data
27. 3 Loading a filter combination Step Action 1 Click the Filter Module button in the main window U FiterModuie Click the Load Filter Module button Select a filter module to load in the Load Filter Module dialog box Click the OK button Click the OK button to return to the main window CAUTION When removing the filter module make sure to click the Filter Module button and remove the filter module after the window changes to the Filter Settings window If the filter module is forcefully removed the area where the filter comes in contact with the PMTs may be damaged Typhoon FLA 7000 User Manual 28 9607 62 AC 61 8 Filter module settings 8 4 Installing and replacing filters 8 4 Installing and replacing filters Remove the filter module from the scanner Step Action 1 Click the Filter Module button in the main window UY Fitter Module Astatus message appears Wait until the message is closed before proceed ing to the next step 2 Open the filter change door 3 Pull filter module straight out 62 Typhoon FLA 7000 User Manual 28 9607 62 AC 8 Filter module settings 8 4 Installing and replacing filters Remove and replace filters in the filter module Step Action 1 Press the notch on the bottom of the filter module and pull out the filter Push the new filter in until it locks in place Typhoon FLA 7000 User Manual 28 9607 62 AC 63
28. 4 3 Connections CAUTION e Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed e Use only the Typhoon FLA 7000 Control Software during reading Electrical power Connect the power cord of the Typhoon FLA 7000 to a grounded power outlet WARNING Use only power cords delivered or approved by GE Healthcare 36 Typhoon FLA 7000 User Manual 28 9607 62 AC 5 Installing Typhoon FLA 7000 Control Software 5 Installing Typhoon FLA 7000 Control Software Administrator privileges required All tasks related to software installation or uninstallation require a computer account with administrator privileges 5 1 Installation sequence Software installation is performed in the following sequence 1 Install the USB control driver 2 Install the USB function driver Windows XP only 3 Install the Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software Windows XP Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows XP Step Action 1 Disconnect Typhoon FLA 7000 from the computer 2 Open the control panel and select Printers and Other Hardware 3 Click Add Hardware to open Add hardware wizard 4 Click the Next button in Add hardware wizard 5 Select Yes have already connected the hardware and click the Next
29. Business SP2 32 bit or Microsoft Windows 7 Professional 32 bit Internal memory 1GB Processor Intel Core 2 Duo processor Hard drive 80 GB Monitor resolution 1280 x 1024 pixels Other requirements One USB 2 0 port DVD ROM drive 10 Typhoon FLA 7000 User Manual 28 9607 62 AC 1 Introduction 1 4 Typhoon FLA 7000 Control Software Overview of the main window The Typhoon FLA 7000 Control Software is used to control use and supervise the Typhoon FLA 7000 S Typhoon FLA 7000 CONTROL SOFTWARE imagination gt work Part Function 1 Phosphorimaging button click to read a storage phosphor screen 2 Fluorescence button click to read a fluorescent sample 3 Digitization button click to perform digitization 4 Method button click to register or erase a combination of laser and filter 5 Filter Module button click to change or register a filter 6 Preferences button click to set the image file format scan mode correction mode and to enable or disable the ND filter 7 Filter displays the loaded filters 8 Laser displays the status of the loaded laser units Typhoon FLA 7000 User Manual 28 9607 62 AC 11 1 Introduction 1 4 Typhoon FLA 7000 Control Software Overview of the reader settings window 12 10 ea a Fle Edt ep 1 Save Condition Load Condition 11 Image Folder Crogan Fter GE Meathose Typhoon FLA OUD Date
30. Deveces wath Removable Storage 4 Desttop Liane Poppy Ouk Onve A DVO CO RW Dewe Computer Typncen FLA 7000 v1 2 w OB O bytes tree at 271 MS USER_DRY F Renal eee Fie gane et Fiesel ype Seno Homation rrt 48 Typhoon FLA 7000 User Manual 28 9607 62 AC 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 11 Select the USB Control folder and click the Open button 12 Select the file DevMng and click the Open button oS Losk USB Corts OF oh Bs Name Date modded Type as Files Currently om the Oise 1 Recent Ploces 6 Deviing 1270 2000 34 PM Setup Infc Desktop Libraries Computer we i y Network tee tnt Hes ctype Setup Wtomaton Caf Cree 13 Click the OK button in the Install from disk dialog 14 Click the Next button in the Add Hardware wizard Add Hardware Select the device driver you wart to install for this hardware Select the manufacturer and model of your hardasre device and then click Nest you have disk that contains the driver you want to install click Have Dak Medet FUVLAS USB Device Management Driver Uh Thh driver is not digitally signed Brett 15 Click the Next button once again TLUAVLAS USU Device Managemere Dever To start inntaifing your mew hardware click Nest Typhoon FLA 7000 User Manual 28 9607 62 AC 49 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Ty
31. GE Healthcare Typhoon FLA 7000 User Manual Table of Contents Table of Contents L 52 0 eee ere et ere eo anina err enter oai Terr i 5 1 1 Important user information 5 12 R g l tory INFORTTICION ssssssssssssseosnseisssocossesseisasnseseessoossessocensvvensvesoocsosecsbenssosovesbenssosesedentsevvssiestioreaneeny 6 1 3 The Typhoon FLA 7000 laser SCONMME ssssssssssssccssessssssssssssssscsscssssssssssssussscescessssssssssnssessessessees 9 1 4 Typhoon FLA 7000 Control Software ccsssssssessssssssssssssssssssesssesccsssssssssussssssssecssssssssusssessssecesee 10 2 Safety instructos essiccare ctackiceedace chacksccetacacasetisoasaresaecizadelackssechaceadtteideasseates 15 QU INTHODUCHON ssssanctscissceannanuiecctannamaiinccumsumnanunnuiannnieniunndsmmanains 15 2 2 General FSC CUEIOMS assisssciessscesesicGisescistesacdaaaaasssseedeadesasaaaaa EE NNA 15 2 3 Personal protection 16 Ze LaSer SAE a A E E T 16 25 Rodation Sde aeee a A A 16 2 6 Radiation hazard prevention 17 Deh EEEE EAN S TEE EAE EOE E EA E 18 28 Labelson Typhoon FLA 7 00 cscssssisssscsesssetsacicessoscannsenvsssioesoascescenstevaasbussodoensnnnccevesisnnsabigonecbeathons 19 2 9 Emergency procedures 23 2 10 Recycling and disposal 24 3 System configUratior iskisi castias kide anei neiaen ekiaren kidoel ki deea Enaki a 25 31 P rtsAnd PU CEIOMS sssini iost Bakare tanani EEEREN iana 25 32 Instrument Dody sersemiuna nnn tE ENN E RE I
32. J i os J eet __ e Click the Next button to install the software in the default folder C Program Files e Click the Change button to select a different location Typhoon FLA 7000 User Manual 28 9607 62 AC 51 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 6 Click the Install button IE Typhoon FA 7000 Irstaiisiseid Wizard f Ready to Install the Program The wizned ie ready to begin inetallation Cock Install to begn the rstalaton M you want to review or change any of your instalation settings cick Rack Chek Cancel to ent the ward y ee r a cancel 7 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified programs wants access to your computer Click Allow 8 Click Install this driver software anyway in the Windows Security dialog Windows Secunty gt Don t install this driver software You should check your manufacturer s website for updated driver software for your device i Only install driver software obtained from your manufacturer s website or dnc Uniigned software from other sources may harm your computer or steal infermation v See details 9 Click the Finish button to complete the installation GB Typhoon Fua 7000 irotaiisiseid Wizard InstaBShiekd Wizard Completed The instats
33. Module Settings window Error message Meaning and countermeasure Filter module has not been set up The filter module is not installed or properly Click the filter module button to configured set up the filter module Countermeasure Install the filter module See Section 8 4 Installing and replacing filters on page 62 Please enter a filter name The filter has no name Countermeasure Type a name in the Name field The filter name has already been Thename of the filter is being used for another used Please change the name filter Countermeasure Choose another name for this filter or change the name for the filter al ready having this name The maximum number of the filters No more filters can be stored in the software you can register is 50 Please delete an unnecessary filter before registeringa a new filter Countermeasure Delete a filter before storing anew one Typhoon FLA 7000 User Manual 28 9607 62 AC 93 13 Troubleshooting Errors and warnings in the Method Settings window Error message Meaning and countermeasure Please input a method name No name has been assigned to the method Countermeasure Type a method name in the Name field The maximum number of the methods is 50 The maximum number of methods are stored No more methods can be registered Countermeasure Use an existing method or delete a method before registering a new one This method nam
34. and the sample in the Typhoon FLA 7000 Read the sample Preparations before starting Typhoon FLA 7000 Checklist before starting Typhoon FLA 7000 66 e Typhoon FLA 7000 is placed on a sturdy horizontal surface e Typhoon FLA 7000 is connected to a grounded wall outlet e The air intake fan on the rear side of Typhoon FLA 7000 is unobstructed e The air exhaust fan on the rear side of Typhoon FLA 7000 is unobstructed e There are no objects on top of Typhoon FLA 7000 e There is no stage in the stage rack Typhoon FLA 7000 User Manual 28 9607 62 AC 9 Operation 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software Start Typhoon FLA 7000 CAUTION Do not insert a storage phosphor screen in Typhoon FLA 7000 before turning on the machine If a storage phosphor screen is detected during the self diagnosis of the Typhoon FLA 7000 the sensitivity of the storage phosphor screen may deteriorate and reduce the quality of the scanned data Step Action 1 Push the power switch on the left side of the body to the I position 2 When the power switch is turned on the indicator lamps change as shown below After turning on the power POWER I susv erron swich HE During self diagnosis or startup POWER I ousy emnon adjustment ronen susv emnon Ready for operation Typhoon FLA 7
35. at all items in the packaging list are included Should you find any external damages or if any items on the packaging list are missing notify the delivery company and contact GE Healthcare for further advice Store Typhoon FLA 7000 in an environment according to Appendix B Specifications on page 99 until the product is unpacked and installed 34 Typhoon FLA 7000 User Manual 28 9607 62 AC 4 Installing and moving Typhoon FLA 7000 4 2 Transporting Typhoon FLA 7000 4 2 Transporting Typhoon FLA 7000 CAUTION Typhoon FLA 7000 must be secured before long distance transports Contact GE Healthcare for help and advice before transporting Typhoon FLA 7000 long distances The Typhoon FLA 7000 weighs approximately 62 kg and requires at least two persons to lift and move Precautions before moving the instrument Step Action 1 Turn off the Typhoon FLA 7000 2 Turn off the computer and any peripheral devices 3 Disconnect the power cords and the USB connection 4 3 Connections CAUTION Only authorized service personnel is allowed to install Typhoon FLA 7000 Contact your local GE Healthcare representative for help and advice Communication Step Action 1 Connect a USB cable to the USB port on the left side of the Typhoon FLA 7000 2 Connect the other end of the USB cable to a USB port on the computer Typhoon FLA 7000 User Manual 28 9607 62 AC 35 4 Installing and moving Typhoon FLA 7000
36. at is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information This section lists the directives and standards that are fulfilled by the Typhoon FLA 7000 system 6 Typhoon FLA 7000 User Manual 28 9607 62 AC 1 Introduction 1 2 Regulatory information Manufacturing information Requirements Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rk gatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden Sept 2009 Identity of person authorized to sign See EC Declaration of Conformity Declaration of Conformity International standards This product fulfills the requirements of the following standards Standard Description Notes EN 61010 1 IEC 61010 1 UL Safety requirements for electrical 61010 1 IEC 61010 2 81 equipment for measurement CAN CSA C22 2 No 61010 control and laboratory use 1 EN 61326 1 EMC emissions and immunity Harmonized with VCCI Class A requirements for electrical 2004 108 EC equipment for meas
37. ck the OK button in the Install from disk dialog Click the Next button in the Add hardware wizard Click the Next button again Click the Continue Anyway button in the Hardware Installation dialog Hardware Installation The software you are installing for this hardware FLA LAS USB Device Managment Driver has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system in the future Microsoft stronaly ends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing STP Click the Finish button to complete the driver installation Typhoon FLA 7000 User Manual 28 9607 62 AC 39 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software Windows XP Install the USB function driver Windows XP Step Action 1 Connect the computer and the Typhoon FLA 7000 with a USB cable and turn ON the power switch of Typhoon FLA 7000 The scanner is automatically detected by the computer In the Found New Hardware Wizard dialog choose No not this time Welcome to the Found New Hardware Wizard Click the Next button in the Found New Hardware Wizard dialog Insert the installation DVD Select Install the software automatically Recommended Click the Next b
38. d instali the hardware automata aby Recommended bratal the hardware that manually select from a let Advanced Typhoon FLA 7000 User Manual 28 9607 62 AC 47 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 7 Select Show All Devices and click the Next button nS a From the bat below asliect the type of hardware you sre installing H you do net see the hardware category yeu want chick Show All Devices Common hardware typet SOuplay adapters FLALAS USE Device KADA ATAATAST controlions AP IEEE 12844 compatible printer PTE LIBEL A devices IEEE 1298 Bas host controllers FBilmaging devices If intrars dences E n e 8 Click the Have Disk button wn E Select the device driver you wart to install for this hardware Select the manufacturer and model of your hardware device and then click Next you have a disk that contains the driver you want to install click Have Dak E l yohoon FLA 7000 Control Software Insert the Typhoon FLA 7000 Control Software DVD and click the Browse button i Insert the manufacturer s installation disk and then make sure that the correct drive is selected below ox 10 Select to install the driver from the Typhoon FLA 7000 Control Software DVD Leake JE Computar 2 Mord Disk Deves 2 a HP RECOVERY Oy i
39. dation if the signals of the sample areas are in the correct range Mode Specify the mode for the reading area borders e Select Grid to limit the borders to the grid lines e Select Free for an arbitrary area e Select All to use the entire Fluor stage as the reading area 10 Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual 11 Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 12 File Size the estimated size of the result data file is presented 13 Reading Time the estimated time required for the scan is present ed 14 Top return to the main window 15 Start Scan start the scan The sample must be loaded before starting a scan Typhoon FLA 7000 User Manual 28 9607 62 AC 13 1 Introduction 1 4 Typhoon FLA 7000 Control Software System requirements Parameter Minimum requirement Operating system Microsoft Windows XP Professional SP3 32 bit or Microsoft Windows Vista Business SP2 32 bit or Microsoft Windows 7 Professional 32 bit Internal memory 1GB Processor Intel Core 2 Duo processor Hard drive 80 GB Monitor resolution 1280 x 1024 pixels Other requirements One USB 2 0 port DVD ROM drive 14 Typhoon FLA 7000 User Manual 28 9607 62 AC 2 Safety
40. dialog opens Enter a name for the conditions in the text field and click OK to save Load condition Click this button to load previously saved conditions Clicking the button opens a dialog box with a list of previously saved condition sets Load a set of stored conditions by selecting it in the list then click OK Remove a set of stored conditions by selecting it then click Remove Confirm the removal by clicking the Yes button in the next dialog box Pixel size Set the pixel size for reading Pixel Size Click to select from one of the four types Asample C 25 um with a smaller pixel size is analyzed in finer detail amp 50 pm C 100 pm C 200 pm Typhoon FLA 7000 User Manual 28 9607 62 AC 79 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Latitude This setting specifies the dynamic range The dynamic range that can be detected is larger with L5 than with L4 If the signals of the sample are in the L4 range the density gradation is represented in more detail if L4 is selected Stage Select the stage which will be used in the scan The image under Sampling Area changes to reflect the selection Sampling area Displays the area or areas to be scanned as red boxes e Change the size of the area or areas to be scanned by clicking and dragging the edges of the red box with the mouse e Change the position of the area or areas to be scanned by clicking and d
41. e computer shuts down immediately The run continues but no data is saved 2 10 Recycling and disposal General instructions for disposal When taking the Typhoon FLA 7000 out of service the different materials must be separated and recycled according to national and local environmental regulations Specific instructions for disposal Measure the superficial radio isotope pollution of the instrument body and storage phosphor screen as mentioned in the radiation hazard prevention section of the User Manual If the pollution level exceeds the limit dispose of the instrument body as radioactive waste Otherwise dispose of the materials according to applicable laws and regulations for disposal of industrial waste 24 Typhoon FLA 7000 User Manual 28 9607 62 AC 3 System configuration 3 System configuration 3 1 Parts and functions The parts and their functions are explained separately in this chapter ssi zeron Part Function 1 Power switch located between the AC connector and the USB connector 2 Indicator lamps 3 Knob 4 Lid Typhoon FLA 7000 User Manual 28 9607 62 AC 25 3 System configuration 3 1 Parts and functions Part Function 5 Setting block 6 Filter change door 7 Filter module slot 8 Filter module 9 Filter 10 Phosphor stage 11 Suction rod 12 Protection cover for storage phosphor screen 13 Fluor stage 14 Dig
42. e dialog box that appears see below Typhoon FLA 7000 User Manual 28 9607 62 AC 59 8 Filter module settings 8 Filter module settings Introduction Filter module settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to register these settings When a filter is changed in the filter module of Typhoon FLA 7000 the new or changed filter must be registered in the Typhoon FLA 7000 Control Software A filter which has not been registered can not be used for analysis and is not displayed in the Typhoon FLA 7000 Control Software 8 1 Registering a new filter name Step Action 1 Click the Filter Module button in the main window QY Filter Module 2 Click the Add button The following dialog box appears Name IEEE Icon m y ok 5 Type the filter name in the Name field 4 Select an icon in the Icon drop down list 5 Finalize the filter addition by clicking the OK button 6 Click the OK button to return to the main window 8 2 Saving a filter combination Step Action 1 Click the Filter Module button in the main window Uy Fitermodue 60 Typhoon FLA 7000 User Manual 28 9607 62 AC Step 8 Filter module settings 8 2 Saving a filter combination Action Click the Save Filter Module button Type a name for the filter combination in the File name field Click the OK button Click the OK button to return to the main window 8
43. e has been already used Please change the name The assigned name is being used by a different method Countermeasure Type a different name in the Name field Errors and warnings in the Reader Settings window Error message Meaning and countermeasure Failed to retrieve image data from the scanner Check the scanner connection There was a problem transferring data to the com puter Countermeasure Check the scanner connection to the computer Cannot detect Typhoon FLA 7000 Please check connec tion and power The scanner was not detected by the computer Countermeasure Check that Typhoon FLA 7000 is properly connected Follow the instructions in Chapter 4 Installing and moving Typhoon FLA 7000 on page 34 Typhoon FLA 7000 imager is running self diagnosis mode Please wait The self diagnosis of Typhoon FLA 7000 is running Countermeasure Wait until the self diagnosis is finished the proceed with the scan 94 Typhoon FLA 7000 User Manual 28 9607 62 AC 13 Troubleshooting Error message Meaning and countermeasure A laser error was detected Please retry the scan or contact service There was an error with the laser Countermeasure Try to scan again Countermeasure Restart the instrument and Ty phoon FLA 7000 Control Software then try to run the scan again Countermeasure Scan using a different laser Please select image folder No image folder i
44. ed Proceed by cliking Install this driver software anyway Click the Finish button in the Add Hardware wizard to complete the instal lation Install Typhoon FLA 7000 Control Software and USB function driver Windows Vista Note Step During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action 2 Insert the Typhoon FLA 7000 Control Software DVD Typhoon FLA 7000 User Manual 28 9607 62 AC 43 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software Windows Vista Step Action 2 3 Locate and double click the file Typhoon FLA 7000 msi In the Typhoon FLA 7000 InstallShield Wizard dialog click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Select destination folder in the dialog EY Typhoon UA 7000 intsinis viad E Drstmetson v ter Coch ret to eta te Pa Sete oF ich Oange 2 eta 9 SA tater tatsi Tarao PUA 7000 te Frog me Fane cs e Click the Next button to install the software at the default folder C Pro gram Files
45. eling off making the storage phosphor screen unusable Handle the storage phosphor screen carefully e Do not scratch or bend the storage phosphor screen e Keep the storage phosphor screen free from dust Protect the storage phosphor screen from water and volatile solvents e Dry the sample thoroughly before exposing it e Wrap wet samples in plastic film and make sure that no liquid exits Use double layers if the sample contains volatile solvents e fwater enters the Storage phosphor screen the sensitivity of the storage phosphor screen is reduced e Volatile solvents may deform the protective film on the storage phosphor screen Protect exposed storage phosphor screens from light Protect exposed storage phosphor screens from light until the reading is finished 86 Typhoon FLA 7000 User Manual 28 9607 62 AC 11 About storage phosphor screens 11 3 Handling precautions Store the storage phosphor screen correctly Remove the storage phosphor screen from the Typhoon FLA 7000 main unit when not in use Store the storage phosphor screens in a horizontal position protected from moisture 114 Preparing the storage phosphor screen Procure necessary tools and items Prepare by acquiring the following items e Storage phosphor screen cassette e Radio isotope labeled samples e Gloves e Plastic film e Soft lint free tissues e Ethanol Clean the storage phosphor screen and the cassette Clean the sur
46. face of the storage phosphor screen and the inside of cassette with a soft tissue to remove dust and stains Erase the storage phosphor screen The FLA Image Eraser can erase the storage phosphor screen in around 15 minutes if it is not overly exposed For details refer to the FLA Image Eraser User Manual Typhoon FLA 7000 User Manual 28 9607 62 AC 87 11 About storage phosphor screens 11 4 Preparing the storage phosphor screen Dim the lighting e Dim the ambient light to 20 lux or less before opening or moving the storage phosphor screen without a cover e Protect the phosphor stage with the protective cover when removing an exposed storage phosphor screen from the cassette and placing it on the Phosphor stage Set the exposure time Set the exposure time so that the exposure finishes right before the reading starts Note Tip The exposure time of the storage phosphor screen is approximately one twentieth of the time required for X ray film Take this into consideration when setting the initial exposure time For increased image quality minimize the time between exposing the storage phosphor screen and reading it with the Typhoon FLA 7000 11 5 Exposing the storage phosphor screen Step Action 1 2 88 Erase the storage phosphor screen completely Wrap the radio isotope sample with plastic film Make sure not to wrinkle the wrapping film Note When using a tritium storage phosphor screen
47. following if an error occurs Step Action 1 Take note of the error code and error message on the monitor 2 Turn off the power to Typhoon FLA 7000 and the computer then turn them on again after about ten seconds 3 Try to perform the action again If the error persists contact your GE Healthcare representative General errors and warnings Error message Meaning and countermeasure 92 Failed to open User Manual Please note that a PDF reader e g Adobe Reader is needed Failed to open End User License Agreement Please note that a PDF reader e g Adobe Reader is needed Failed to open Getting Started Please note that a PDF reader e g Adobe Reader is needed The software required to read the online PDF documentation is miss ing Countermeasure Install software for viewing PDF documents The disk capacity is insufficient The available disk space is insuffi cient Countermeasure Free up disk space on the computer Countermeasure Store the data on a different disk No disk space See above Typhoon FLA 7000 User Manual 28 9607 62 AC 13 Troubleshooting Errors and warnings in the main window Error message Meaning and countermeasure Cannot detect Typhoon FLA 7000 The scanner is not detected Please check connection and power Countermeasure Check that the scanner is turned on and connected to the computer Errors and warnings in the Filter
48. held wizard has successfully netaled Typhoon FLA 7000 Chick Finish to ext the wizard Launch Typhoon FLA 7000 o 52 Typhoon FLA 7000 User Manual 28 9607 62 AC 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software Before you begin Log in using a Windows account with administrator privileges Uninstalling Typhoon FLA 7000 Control Software under Windows XP Step Action 1 Open the control panel and select Add or Remove Programs 2 Select Typhoon FLA 7000 and click the Remove button wren anae Aswan Vat Vad 1801 E vec tenet Cogn E Vern tere Lerm Sattar Units 1B Lote for Weed bterrnt Gopher 8 XRT VA D becats Updo bor erate remat Coch 8 HOOT 3 Confirm the uninstallation by clicking Yes when prompted Note Correction files created during calibration such as shading data are required by the Typhoon FLA 7000 Control Software They are not deleted during the uninstallation and remain in the Data folder of Typhoon FLA 7000 Control Software folder Typhoon FLA 7000 User Manual 28 9607 62 AC 53 6 Uninstalling and upgrading Typhoon FLA 7000 Control Software Uninstalling Typhoon FLA 7000 Control Software under Windows Vista Step Action 1 Open the control panel and Select Uninstall a program under Programs 2 Select Typhoon FLA 7000 and then click Uninstall a OOA marma roye reye mitaaa 2
49. ing in Click the magnifying glass symbol and left click the display area or Click the magnifying glass symbol and right click the display area or select a magnification ratio further down the Magnification drop down list Typhoon FLA 7000 User Manual 28 9607 62 AC Zooming out Click the magnifying glass symbol and right click the display area or Click the magnifying glass symbol and left click the display area or select a magnification ratio further up the Magnification drop down list 81 10 Reading conditions display parameters and other settings 10 2 Display parameters explained Intensity graph The intensity graph displays the intensity levels of the image in histogram format The Low and High values below the graph correspond to the range of light intensities displayed These values are displayed in the graph as vertical red lines To display a larger or smaller interval of light intensities drag the lines using the mouse to adjust the Low and High values Curve Select the type of tone curve used in the intensity graph Setting Description Exponential The light intensities are displayed using an exponential tone curve Linear The light intensities are displayed using a linear tone curve Sigmoid The light intensities are displayed using a sigmoid S shaped tone curve Auto Range Scope Check this option to automatically adjust the range of light intensities for opti
50. itizing fluorescent plate 15 Membrane weight included in Fluor stage 26 Typhoon FLA 7000 User Manual 28 9607 62 AC 3 System configuration 3 2 Instrument body 3 2 Instrument body Power switch The power switch is located on the left side of the Typhoon FLA 7000 Inlet Cooling fans The instrument has two cooling fans on the back side CAUTION Do not block the cooling fans with a wall or objects If they are blocked the instrument may become damaged Typhoon FLA 7000 User Manual 28 9607 62 AC 27 3 System configuration 3 2 Instrument body Air intake fan Exhaust fan Indicator lamps Three indicator lamps indicate the instrument status 28 Typhoon FLA 7000 User Manual 28 9607 62 AC 3 System configuration 3 2 Instrument body Stage setting block Stage setting block Filter module The filter module is located behind the filter door Filter module Filter module slot 5 Filter change door Typhoon FLA 7000 User Manual 28 9607 62 AC 29 3 System configuration 3 2 Instrument body USB port The USB port is located on the left side of Typhoon FLA 7000 USB label 3 3 Accessories Digitization plate included The digitization plate is a fluorescent plate that emits light when exposed to a laser beam This plate is used when the digitization mode is used 30 Typhoon FLA 7000 User Manual 28 9607 62
51. mum display results 10 3 Other settings Preferences gt Preferences Click the Preferences button in the main window to display a tabbed dialog box where various options related to sample reading can be viewed and changed Not all options are relevant in all scanning modes 82 Typhoon FLA 7000 User Manual 28 9607 62 AC 10 Reading conditions display parameters and other settings 10 3 Other settings Preferences e eee x al p For fluorescence moc de ND Fiter Scan Mode G ot G Standard Con Quick tonite beter te rwar G Gimme rte a D G imaga He gn PF brag He C are septate f a A Setting Description Correction Mode Auto Use specific image shading correction data that were adjusted in accordance with each laser Manual The correction method must be selected individually in the Reader Settings window Note The correction mode settings list is registered by a serviceman Please contact the dealer where you purchased Typhoon FLA 7000 or contact GE Healthcare ND Filter Enables the use of an ND filter to adjust the light intensity Use the ND filter if the signal from the sample is very strong Select On to enable the ND filter Select Off to disable the ND filter Scan Mode Determines the scanning speed Standard Mode 200 um 210 s 100 um 210 s 50 um 330 s 25 um 450 s Quick Mode 200 um 150 s
52. n FLA 7000 nstaltStockd Wizard s Wekome to the InstalShiek Wizard for Typhoon FLA 7000 The instattchveld R Wizard wil natal Typhoon PLA 7000 on you computer To contnue cick Next WARNING This program a protected by cogyngnt law and iternabons Peabes SSS CE ee E 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information GRP Typhoon FLA 7000 InstallShocla Weard s Lu eme Agreement Pame raad the following krae ayeeman caret dy GE Healthcare STANDARD SOFTWARE END USER LICENSE AGREEMENT FOR LIFE SCIENCES SOFTWARE PRODUCTS IMPORTANT PLEASE READ CAREFULLY THIS S THE LICENSE AGREEMENT THAT END USER IS REQUIRED TO mieh aopa USING GE HEALTHCARE SOFTWARE OR E a oe THIS LICENSE BEFORE PROCEEDING Wi DOWNLOADING ANDOR ipt the terms in the Kerwe agreement gna 1 gio not accept the terms n the kerse agreement trata eect SS SSS Se os J ee m De Select I accept the terms in the license agreement and click the Next button 5 Select the destination folder in the dialog IE Typhoon FUA 7000 Irotaisiseid Wizard Destination Folder ick Next to instal to thie fader or cick Change to inetal to a different foicker natal Typhoon PLA 7000 to C Program Pies Orange i J J J
53. n Typhoon FLA 7000 Laser and class Wavelength Maximum power LD laser class 3B 473 nm 20 mW CW SHG laser class 3B 532 nm 10 mW CW LD laser class 3B 635 nm 45 mW CW LD laser class 3B 650 nm 80 mW CW Note The beam divergence of all laser modules is collimated Regulatory compliance of connected equipment Any equipment connected to the Typhoon FLA 7000 should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked 1 3 The Typhoon FLA 7000 laser scanner The compact Typhoon FLA 7000 is an image analyzer that offers high performance multifunctionality and high speed reading Benefits include Typhoon FLA 7000 User Manual 28 9607 62 AC 9 1 Introduction 1 3 The Typhoon FLA 7000 laser scanner e Detection of high sensitivity autoradiographs using storage phosphor screens e Four lasers and multiple filters enable the use of a variety of fluorescent dyes and detection methods e Data is aquired through high resolution sensitivity and wide range linearity e Compatibility with various types of samples for example fluorescent labels fluorescent dyes CBB stain silver stain X ray film and storage phosphor screens 1 4 Typhoon FLA 7000 Control Software System requirements Parameter Minimum requirement Operating system Microsoft Windows XP Professional SP3 32 bit or Microsoft Windows Vista
54. n to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Disconnect Typhoon FLA 7000 from the computer 2 Open the control panel and click Hardware and Sound 3 Click Device Manager under Devices and Printers 46 Typhoon FLA 7000 User Manual 28 9607 62 AC 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 4 Select Action Add legacy hardware 5 Inthe Add Hardware dialog click the Next button Add Hardware Welcome to the Add Hardware Wizard Tha wezeg helps you matal dewer software to support older devices thet do met support Plug and Play end whch are not maomatk ady recognced by Windows You should omy use tha weeard you sre an advanced user or you have been deected here by technical support AL your hardware came with an installation CD it is recommended that you click Cancel to coce this wizaed and sse the manufacturer s CD to instal this hardware To continue cick Niet Ge God L 6 Select Install the hardware that I manually select from a list Advanced and click the Next button Add Hardware The wizard can halp you install othar hartuara The wizard can search for other hardware and automatically instali it for you Or if you know exactly which hardmare medel you went te matal you can select it from a bst What do you wart the wicard to de Search for an
55. nk or in humid or dusty environments Fire and electric shock could result WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could result gt gt gt gt 18 Typhoon FLA 7000 User Manual 28 9607 62 AC 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 2 8 Labels on Typhoon FLA 7000 Typhoon FLA 7000 serial number The Typhoon FLA 7000 serial number is located on a label on the back of the instrument GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER Model Typhoon FLA 7000 Serial No 123456 Frequency 50 60 Hz Supply Voltage 100 240 V c US Max Current 3 0 1 5A LISTED Protection Class IP20 PABORATORY el Manufacturing Year ACESO Manufacturer FUJIFILM Corporation TOKYO 106 8620 JAPAN MADE IN JAPAN 405N101498 Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation EMC in Australia and New Zealand The system complies with applicable European directives The system complies with the requirements for electromagne
56. o save image data This field is mandatory in order to start reading a sample Note The method name is automatically added to the specified name and is saved to a file Comment Enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software Method Set the method to use in the scan i he pull down menu select the method that Method Cys ponds with the sample The selected laser Cy3 after combination is displayed below the EtBr ROX Deep Purple Pro Q Diamond Alexa Fluor 633 DDAO Silver Stain CBB v In fluorescent mode up to 4 scans can be performed in a row all with individual methods PMT PMT 500 v 00 1000 You may set the voltage to be applied to the pho to multiplier tube PMT as an integral value within the predetermined range The larger the value is the higher the sensitivity 500V Value 1000v Smal lt Large Se sitivity Low lt i rich 78 Typhoon FLA 7000 User Manual 28 9607 62 AC 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Plus and minus buttons fluorescent mode only Click to increase the number of scans to decrease the number of scans Top button Click this button to return to the main window Save condition Click this button to save the current conditions settings to a file Save the current conditions by clicking Save Condition A text
57. ows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action Disconnect Typhoon FLA 7000 from the computer Open the control panel and click Classic View in the upper left corner Typhoon FLA 7000 User Manual 28 9607 62 AC 41 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software Windows Vista Step Action 3 Open Add Hardware 4 In the Add Hardware dialog click the Next button 5 Select Install the hardware that I manually select from a list Advanced and click the Next button 6 Select Show All Devices and click the Next button ERE 7 Click the Have Disk button 8 Insert the Typhoon FLA 7000 Control Software DVD and click the Browse button 9 Select to install the driver from the Typhoon FLA 7000 Control Software DVD 10 Select the USB Control folder and click Open 42 nun woepiwmenw gt OF i Ge fo fare fm tee oa gt Typhoon FLA 7000 User Manual 28 9607 62 AC Step 5 Installing Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software Windows Vista Action 11 12 13 14 15 16 Select the file DevMng and click the Open button Click the OK button in the dialog Install from disk Click the Next button in the wizard Add hardware Click the Next button once again The following warning is display
58. phoon FLA 7000 Control Software Windows 7 16 The following warning is displayed Proceed by clicking Install this driver software anyway f Windows Secunty s Don t install this driver software You should check your manufacturer s website for updated driver software for your device i Only install driver software abtained f Sic Untigned software from other so information v See details 5 2 17 Click the Finish button in the Add Hardware wizard to complete the installation Add Hardware Completing the Add Hardware Wizard The totowing hardware was instabes F FALAS USE Derice Management Driver L Winsen has finshed mitating the software for thes Serce To close thit wizard click Finish Installation of the Typhoon FLA 7000 Control Software and USB function driver Windows 7 Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Insert the Typhoon FLA 7000 Control Software DVD 2 Locate and double click the file Control Software msi 50 Typhoon FLA 7000 User Manual 28 9607 62 AC 5 Installing Typhoon FLA 7000 Control Software 5 4 Install Typhoon FLA 7000 Control Software Windows 7 3 Inthe Control Software InstallShield Wizard dialog click the Next button JB Typhoo
59. phor Gis50nm P Pro Q Diamond Gj532nm_ s 0580 E Select the desired method to edit Click the Edit button Enter the desired changes Click the OK button Finalize the changes by clicking the OK button in the Method Settings dialog box Note Default methods cannot be edited Names of default methods are surrounded by square brackets Deleting a method Note Default methods cannot be deleted Names of default methods are surrounded by square brackets Typhoon FLA 7000 User Manual 28 9607 62 AC 7 Registering editing and deleting methods 7 3 Deleting a method Step Action I Click the Method button in the main window The Method Settings dialog box appears Method Settings E Method Settings Method List Cy3 0580 Cy5 Ejs35nm s R670 DDAO Eys35nm JIR670 Deep Purple E 532nm 0580 EtBr G9532nm 0580 FAM E473nm Weys20 FITC 473nm BLY520 Phosphor Gs5onm BLIP Pro Q Diamond G532nm s 0580 E Delete Edit Add 2 Select the desired method to delete 3 Click the Delete button 4 Click the Yes button A confirmation dialog appears In the screenshots below the method name given is no name GZ Aeyou sure you want to delete this method no name 5 Finalize the changes by clicking the OK button in the Method Settings dialog box Note To undo the filter deletion click the Cancel button in the Method Settings dialog box Click Yes in th
60. r by service personnel Suction rod included 32 The suction rod is used to lift up or place the the storage phosphor screen on the phosphor stage Press the suction rod against the storage phosphor screen Place a finger on the end of the suction rod then lift up the storage phosphor screen Release the finger to release the storage phosphor screen Typhoon FLA 7000 User Manual 28 9607 62 AC 3 System configuration 3 3 Accessories Suction rod Titer plate TP plugin included The TP plugin is used when reading titer plate samples USB cable included The USB cable is used to connect Typhoon FLA 7000 to a computer Typhoon FLA 7000 User Manual 28 9607 62 AC 33 4 Installing and moving Typhoon FLA 7000 4 Installing and moving Typhoon FLA 7000 CAUTION Only authorized service personnel is allowed to install Typhoon FLA 7000 Contact your local GE Healthcare representative for help and advice 4 1 Site requirements Note The Typhoon FLA 7000 is intended for indoor use only Parameter Requirement Power supply 100 to 240 VAC 3 0 to 1 5 A Line frequency 50 to 60 Hz Placement Stable horizontal surface out of direct sunlight Ambient temperature 10 to 30 C Humidity 20 to 75 non condensing Maximum altitude 2000 m above sea level Initial delivery inspection Upon receiving Typhoon FLA 7000 e inspect the package for external damages e check th
61. rage phosphor screen next to the phosphor stage 5 1 Press one end of the suction rod against the storage phosphor screen 2 Cover the other end of the suction rod with a finger 3 Lift up the storage phosphor screen by the suction rod and place the storage phosphor screen on the phosphor stage 4 Release the storage phosphor screen by releasing your finger from the suction rod 4 Place the storage phosphor screen on the back of the phosphor stage with the white or blue reading surface of the storage phosphor screen facing up 72 Typhoon FLA 7000 User Manual 28 9607 62 AC 9 Operation 9 7 Placing the stage in Typhoon FLA 7000 9 7 Placing the stage in Typhoon FLA 7000 Placing the Fluor or Multi stage in the Typhoon FLA 7000 Step Action 1 Pull the knob up and open the lid 2 Position the stage so that the black triangle mark is aligned with the corre sponding mark on the setting block Fit the setting block pin in the hole of the stage 3 Pull the knob up and close the lid Typhoon FLA 7000 User Manual 28 9607 62 AC 73 9 Operation 9 7 Placing the stage in Typhoon FLA 7000 Placing the phosphor stage in Typhoon FLA 7000 Step Action 1 Pull the knob up and open the lid 2 Position the stage so that the black triangle mark is aligned with the corre sponding mark on the setting block 3 Fit the setting block pin in the hole of the stage 4 Pull the knob up and close the lid
62. ragging the red box with the mouse Mode Sets the selection behavior of Sampling area Setting Description Grid The sampling area is increased or decreased in steps corresponding to the grid of the stage or the positions of the titer plate plugin Free The sampling area is increased or decreased in a stepless fashion All The sampling area is expanded to the whole readable surface of the stage or titer plate plugin is selected File Size This area displays the estimated size of the image file as determined by the current settings 80 Typhoon FLA 7000 User Manual 28 9607 62 AC 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Reading Time This area displays the estimated reading time as determined by the current settings Scan button Click this button to start scanning the sample or samples using the current reading conditions 10 2 Display parameters explained Upper limit Lower limit EEEE deretan wei seadm teri Intensity graph a v 3 Curve Auto Range Scope Magnification ssm j A see eee en Magnification Select a ratio to zoom in and out of the display area If the magnification is high scroll bars appear on the right and bottom edges of the display area Use these scrollbars to view different parts of the display area There are several ways to zoom in and out of the display area see the table below Zoom
63. s 97 A Default sample detection methods 635 nm laser Method name Filter type Cy5 R670 Alexa Fluor 633 R670 DDAO R670 650 nm laser Method name Filter type Phosphorimaging IP CBB R710 98 Typhoon FLA 7000 User Manual 28 9607 62 AC B Specifications Appendix B Specifications Wavelengths supported e 473nm e 532nm e 635nm e 650nm Filter types supported IP imaging plate included 0580 included R670 included Y520 included R710 optional accessory Scanning specifications Reading mode Scanned image size Storage phosphor 20 cm x 40 cm maximum slide 18 cm x 36 cm valid field angle Fluorescent 24 cm x 40 cm maximum 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size Digitization 24 cm x 40 cm maximum 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size Parameter Data Pixel size 25 50 100 or 200 um selectable Typhoon FLA 7000 User Manual 28 9607 62 AC 99 B Specifications Parameter Data Gradation 16 bit Dynamic range Five orders of magnitude Maximum image capac 307 2 MB 25 um 76 8 MB 50 um ity 19 2 MB 100 um 4 8 MB 200 um Dimensions amp weight Unit Dimensions mm Weight kg wxdxh Reading block 940 x 556 x 360 62 projections not in cluded Storage phosphor screen cassette 227 5X4275x15 Approx 1 Phosphor stage 4
64. s 76 912 TIME oif TYDMOGM FLA 7000 ssh sssssctisaciseedeSssssesteveySececistegecsehzeeasnvsassbestessciteesaseassistvusladiscostitiotts 76 10 Reading conditions display parameters and other settings 0006 77 10 1 Reading conditions explained 77 10 2 Display parameters explained 81 10 3 Other Settings susineria oaii 82 11 About storage Phosphor SCreeNS scsecccrsseonicscteretineencianeumciine 85 TLL VEO AU CUI sip nasassssisacssazzzensanansalggscongasscsjtzsfieaanacdssnscnasnanlagdgusteanstsnonsssshansscgassesiotasssnsssectslsseeCbetdusnastend 85 11 2 Compatible storage phosphor screens 85 DTS Handling precauti NSi sass sscsssssndsessissvsso seooensdsasevsn cass ainni 85 11 4 Preparing the storage phosphor screen vsesesssesssssscsssssssssssssessesscssscssssssssssssessesecsessssssssuseseseees 87 11 5 Exposing the storage phosphor Screen csssesssessssssccssssssssssssesssssessscsssssssssssssssesecssssssssssusesecssees 88 12 MGIMRONGINGE ciesciccstessectacksceesedectsckcatacate sus ctsazctavsscvecaxebesbssvesaeatesecteeceretebetavhbaaeetazes 90 13 Troubleshooting nee lee Ee ra tyr ey ene erie Pe Orn rier 92 A Default sample detection Methods csssssssssscsesssscsssccesesceessssseseseeeseces 97 B CIN CCN ccs teceeccaicaerscatexysicvenesesactesaxeeuvctavencecies Tiru AEE EAE EAEra raini 99 4 Typhoon FLA 7000 User Manual 28 9607 62 AC 1 Introduction 1 Introduction In this chapter This chapter contains important
65. s selected Countermeasure Select an image folder by clicking the Browse button and navigating to a suitable folder Please input a file name No name has been assigned to the image file Countermeasure Type a name in the File Name field Please set PMT Voltage value to 500 1000 The PMT voltage setting is outside the permitted range Countermeasure Type a PMT voltage value be tween 500 and 1000 in the PMT field Please give a name to the condition No name was given to a condition before saving Countermeasure Type a name in the Condition Name field Failed to retrieve image data from the scanner Check the scanner connection The image data could not be retrieved from the scanner Countermeasure Check that the scanner is properly connected to the computer then try to run the scan again Countermeasure Disconnect all other USB devices from the computer and run the scan again Countermeasure Restart the computer and Ty phoon FLA 7000 Control Software then try to run the scan again The stage is not properly in serted Please insert the stage in its correct position The stage is not properly inserted Countermeasure Insert the stage properly Typhoon FLA 7000 User Manual 28 9607 62 AC 95 13 Troubleshooting Errors and warnings in the scan progress window Error message Meaning and countermeasure a lower PMT voltage Overexposure error occurred
66. sseccesssssssusseeses 60 8 2 SAVING A filter comDiNdtON sssesesiiiisnenoniimousmnsnonimianin na 60 Typhoon FLA 7000 User Manual 28 9607 62 AC 3 Table of Contents 8 3 Loading AILS CORBI OI issoria aaan a 61 84 Instalingiand replacing fiter S sass ccscccstosssssssssvscasevsasvascseazsctscevenadavovssusecceeceactinvaas dass aan 62 eE e1 eol RE ETE E A E E EEE EE 66 OT OPErdtlon OVEMVIEW in nessinssnnni ni R a aiaia 66 9 2 Preparations before starting Typhoon FLA 7000 66 9 3 Starting Typhoon FLA 7000 and Typhoon FLA 7000 Control Software wees 67 94 Selecting TAS SCAN ModE nnsianennnnn rnain na EAA ANA 68 9 5 Setting the redding COnditONS ssrsssiniisesssussnniununassisaninss 69 9 6 Placing the object to be scanned on the stage eseesssssessssssessssstesssttenssnesssnsssneeesneeeseeeeees 69 9 7 Placing the stage in Typhoon FLA 7000 uui eessssssssssssessssssssccssssssssssuessessessessssssussesesseceesssssssunsees 73 9 8 Scanning th sdmnplE a sescacacssccovessussanassvssessaedsioeiistzsencssssssssazesghbbecsbeduessstasesceessessbbouseontesnieessdtetsubass 74 9 9 Adjusting the display parameters ou eeccsssssssssssssssssssessessccssssssssussssssssessssssssssssseesssessecesssssssseeees 75 9 10 Saving the image data using a different file NAME ou eeseesessssesssssesssssssenstenesteneeesneesees 76 9 11 Viewing the image file in the analysis application oo eeesssssssesssessessccsssssssssusseessescccsssesssnusnee
67. the object to be scanned on the stage CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples Placing a gel sample on the Fluor stage Note e The sample must not be thicker than 30 mm For solutions the maximum height is 4 mm e Make sure there are no bubbles or gaps on the contact surface Step Action 1 Place the sample on the Fluor stage Typhoon FLA 7000 User Manual 28 9607 62 AC 69 9 Operation 9 6 Placing the object to be scanned on the stage Placing a titer plate on the Multi stage Step Action 1 Place the titer plate frame on the Multi stage P L J Z irFiasj ristisix ictmnminioie Placing a gel sample with glass on the Multi stage Step Action di Place a gel sample with glass on the Multi stage 70 Typhoon FLA 7000 User Manual 28 9607 62 AC 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 2 Carefully fold down the spring loaded glass holders on the glass 5 Tighten the screws on the glass holders as necessary Placing the storage phosphor screen on the phosphor stage CAUTION Always wear cotton gloves when handling storage phosphor screens Step Action 1 Turn down the lighting in the room Typhoon FLA 7000 User Manual 28 9607 62 AC 71 9 Operation 9 6 Placing the object to be scanned on the stage Step Action Z Place the cassette with the exposed sto
68. tic compliance Typhoon FLA 7000 User Manual 28 9607 62 AC 19 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 Labels concerning use of hazardous substances Meaning This symbol indicates that the waste of electrical and electronic equipment asd must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufac mam turer for information concerning the decommissioning of equipment Labels concerning laser light Meaning oid exposure to the laser he lid is open CAUTION Class 3B Laser product when Ta f SCRBUT 1 9 D 7ERRTEEI23B open and interlock defeated Avoid expo LOBIECERI CLEEN sure to the beam CAUTION CLASS 3B VISIBLE AND INVISIBLE LASER RADIATION WHEN OPEN AND INTERLOCKS Do not attempt to defeat the safety inter EFEATED AVOID EXPOSURE TO THE BEAM locks under the sample lid or behind the ATTENTION r RAYONNEMENT LASER VISIBLE ET INVISIBLE filter door or otherwise try to gain access DE CLASSE 3B A L OUVERTURE ET QUAND LE VERROUILLAGE EGT DEJOUE to the interior of the instrument through any other opening Exposure to laser light can cause injury Viewing the laser light directly can cause blindness 20 Typhoon FLA 7000 User Manual 28 9607 62 AC 2 Safety instructions 2 8 Labels on Typhoon FLA 7000 Labels at the front of Typhoon FLA 7000 NITWIORY F R FLA 7000A
69. urement FCC Part 15 B Class A control and laboratory use ICES 003 Class A EN ISO 12100 1 12100 2 Safety of machinery Basic con Harmonized with cepts general principles for de 2006 42 EC sign EN ISO 14121 1 14121 2 Safety of machinery Principles Harmonized with of risk assessment 2006 42 EC EN 60825 1 IEC 60825 1 Safety of laser products Harmonized with 2006 95 EC USA 21 CFR Chapter Sub Safety of laser products chapter J Part 1040 10 Laser Products Typhoon FLA 7000 User Manual 28 9607 62 AC N 1 Introduction 1 2 Regulatory information CE Conformity CE Marking This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of Conformity is available on request Directive Title 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive CE The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is e used as a stand alone unit or e connected to other CE marked GE Healthcare instruments or e connected to other products recommended or described in the user documentation and e used inthe same state as it was delivered from GE Healthcare except for alterations described in the user documentation Note This equipment has been tested
70. utton in the Found New Hardware Wizard dialog Click the Finish button to complete the installation Install Typhoon FLA 7000 Control Software Windows XP Step 1 2 40 Action Insert the Typhoon FLA 7000 Control Software DVD Locate and double click the file Typhoon FLA 7000 msi In the Typhoon FLA 7000 InstallShield Wizard click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Typhoon FLA 7000 User Manual 28 9607 62 AC Step 5 Installing Typhoon FLA 7000 Control Software 5 2 Install Typhoon FLA 7000 Control Software Windows XP Action 5 Select destination folder in the dialog e Click the Next button to install the software at the default folder C Pro gram Files e Click the Change button to install to a different folder Click the Install button in the installation dialog Click the Finish button to finish the installation of Typhoon FLA 7000 Control Software 5 3 Install Typhoon FLA 7000 Control Software Windows Vista Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows Vista Note Step During software installations you may be asked to confirm your actions in a dialog with the text Wind

Typhoon™ FLA 7000

Contents

Download Pdf Manuals

Related Search

Related Contents

Typhoon&trade; FLA 7000

Contents

Download Pdf Manuals

Related Search

Related Contents

Typhoon™ FLA 7000