DynaChip Processor Manual - Thermo Fisher Scientific
Contents
1. Processor Maintenance 4 1 Tube Rinsing Daily rinsing Daily rinsing of all DynaChip Processor tubing is required for reliable test runs First step of the script that performs the runs fills all tubing and channels with appropriate buffer Tubing should be washed if the machine will not be used in the following 24 hours Washing can also be carried out from the main screen of the Processor module choose the Instrument button e During this pre test procedure tubing and channels are rinsed with the appropriate assay buffer provided in one of the external Buffer Bottles The Wash unit 5b of Liquid Handling Head is positioned above the right of two rear position in the Reagent Reservoir Rack 4b to dispense and collect wash buffer NOTE Ensure that one empty reservoir bottles have been placed into both rear positions of rack 4b e After completion of the day s experiments and if the machine will not be used in the following 24 hours select the button that display wash tubing The software give instruction with what to do Make sure that the wash bottle is filled with water The wash procedure can also be started from the service section of the software see software manual Ensure that all tubings are properly inserted into the appropriate bottles and or After completion of the rinsing procedure remove tubing from Wash Buffer Bottles and start wash procedure dry without the application of any buffer to allow the system to2. Adapter for insertion of the Pipetting part 5a enabling the movement of the ArrayStrip Frame in XY direction along the tracks Well reading position EY e MES y B El Fig 4 The ArrayStrip frame can be moved in XY direction for moving into reading position 2 2 1 Installation Instrument Unpacking 8 Setup Unpacking To unpack the DynaChip Processor instrument the transportation box needs to be in an upright position to open The DynaChip Processor instrument needs at least 600 mm x 800 mm space and a safe and stable standing position at bench level CAUTION Heavy weight of approx 30 kg The DynaChip Processor lid swings upwards when opened the height is 970mm from the instrument base to the top of the lid Carefully lift and place the DynaChip Processor in position CAUTION Heavy weight of approx 30 kg Inspect for any obvious signs of damage and report any damage immediately Ensure free ventilation of the instrument The ventilation slots on side and bottom walls must not be covered Before any further installation of instrument and computer ensure that the surrounding temperature is ambient Allow temperature equilibration for at least 6 hours in order to avoid condensation collecting within the instrument Check level of cooling liquid Tf necessary top up cooling liquid see paragraph 4 2 For transportation the instrument is secured with transportation interlocks Open lid and
3. for anchoring the Sample Plate and Tip Rack Metal pins 2 2 1 Rack holding Sample Plate and Tip Racks Unpack tray holding Sample Plate and Tip Racks and fix onto the 8 metal pins for mounting Insert the tray with its movable clamps facing the front side of the DynaChip Processor see fig 9 The clamps enable the secure fixing of the Tip Racks which is necessary during processing Fig 9 At the front mount the tray holding Sample Plate and Tip Racks onto metal pins and ensure that the movable clamps are facing the front of the instrument movable clamps Loading of the tray with one or two Tip Racks can be done inside or outside of the DynaChip Processor see also paragraph 3 2 For loading open clamps and insert Tip Racks Close the clamps to fix the racks securely into place which is necessary during processing 2 2 2 Reservoir Racks e Unpack both Reagent Reservoir Racks and place them in their appropriate positions 4a amp b see also figs 2 amp 8 Make sure these are placed correctly the notched corners of the racks need to face the rear of the instrument Fig 10 Inserted reagent Reservoir Racks here already containing reservoir bottles ensure correct positioning marked by notched corners of the racks Notched corners must face the rear of DynaChip Processor e Attach frame for waste bags for pipette tips on front side of DynaChip Processor and insert waste bag d Fig 11 Fix frame for
4. the processor this is used for accessing the archive Ensure that instrument and control computer are properly connected and turned ON Check if green status LED on the front part is ON If not fuses might have to be replaced refer to paragraph 4 4 Test run was aborted Ensure that cover has not been opened during test run This will lead to test abortion In all other cases note error code and message and contact customer service for further support Test run was aborted by accidental Restart ASP instrument After its initialisation procedure only a new test pushing the red emergency run can be started No results Inappropriate liquid volume ensure the user follow the DynaChip IFU In all other cases as listed below contact Technical Service for further support No transfer of liguid by pipetting part during dispensing steps No transfer of liguid or transfer of insufficient amounts of liguid during dispensing steps No aspiration of liguid during aspirating steps Pipettor Unit does not work properly or cannot pick up pipette tips No cooling of reagent reservoir Control if cooling unit was turned ON green status LED of its power rack 4a supply must be ON Check for correct level of cooling fluid If reguired insert cooling fluid refer to paragraph 4 2 Failure of analysis If image in software shows developed markers on only part of the chip or general precipitated substrate then
5. the instrument The ventilation slots on side and bottom walls must not be covered It is not allowed to use device within explosive atmosphere The DynaChip Processor needs a safe and stable standing position at bench level The DynaChip Processor Workstation is designed to work properly only if the surrounding room temperature is between 15 35 C 59 95 F Important Operation Notes Do not open any of the closed parts of the instrument or the casing Any damage resulting from improper use will result in the loss of Warranty rights Avoid any contamination of the Optical Unit of the instrument with liquids Do not move ArrayStrip Frame by hand Load only new unspoiled unused pipette tips If DynaChip Processor is not in use always keep the lid closed to prevent dust particles from collecting inside the optical components For transportation use only the original DynaChip Processor wrapping Please turn off DynaChip Processor when not in use Warnings To avoid any damage and injury the DynaChip Processor must be switched off immediately in case of abnormal noises smoke or the smell of fire if the power supply cable is damaged if liquid entered the instrument Immediate switch off is performed by pushing the red STOP bottom on the front of the instrument Please inform the customer service if any of these incidents have occurred 17 6 2 Technical Details DynaChip Processor Component
6. waste bags at two slots installed at left front side of the station 2 3 Tube Connecting For dispensing and aspirating steps with the Liguid Handling Head liguid is transferred to and from this head utilising two channels The channels are linked to the external Buffer Bottle and Waste Bottle via tubing connected to the three ports at the rear of the instrument Fig 6 f and Fig 12 For the DynaChip processor only port In1 and Out is used The dispensing channel is linked to the external Buffer Bottle by the In1 port The aspirating channel is linked to the Waste Bottle by the OUT port NOTE During operation the waste port OUT must always be connected by appropriate tubing to an external Waste Bottle NOTE Only use tubing provided with the instrument or specified according to the list in appendix 5 4 nt aq Fig 12 Rear side of DynaChip Processor see also fig 6 f with ports In1 and In2 for the connection of external Buffer Bottle and port OUT for the Waste Bottle OUT port 2 3 1 External Buffer Bottles 1 amp 2 For rinsing and wash steps requiring higher or repeated volumes of buffer or water the Liquid Handling Head needs to be connected the external Buffer Bottles e Connect one end of provided tubing to the Inlet 1 In1 and or Inlet 2 In2 on the rear side of the DynaChip Processor fig 12 e Connect other end of tubing into appropriate external Buffer Bottle s The bottle
7. art position after finishing initialisation procedure Fig 15 Green LED light shining after switching power ON The Red STOP button for immediate abortion of the DynaChip Processor program 12 3 Preparations 3 1 Tube Rinsing If the user fail to wash the tubing and the machine was subsequently left for more than 24 hours a rinse procedure should be carried out as described in the section 4 1 3 2 Tip Racks and Tip Waste Bag Fill Tip Racks with the number of pipette tips required for the next run to be carried out Loading of Tip Racks can be performed inside or outside the instrument Load one racks onto the DynaChip Processor by opening the securing clamps or gates of the rack at front of machine and sliding them into place Close the securing clamps gates there should be an audible sound as they shut If processing 1 11 strips only one rack is required in position 3a For a full 12 strip run a second rack should be placed in position 3b For information on tips for the DynaChip Processor please refer to paragraph 5 3 of the appendix NOTE Load tip racks only with new unspoiled tips 3 3 Cooling Unit If cooling of the Reagent Reservoir Rack at position 4a is required start Cooling Unit at least 15 min before adding any reagent bottle or starting a test run Switch on the Cooling Unit at the rear of the instrument see Fig 5 e A green LED on the power supply of the unit indicates its status 4 DynaChip
8. d with separate Pipetting parts enabling to run two different modes of liquid handling during test processing fig 3 1 Pipetting 5a Dispensing and aspirating of defined liquid volumes up to 250 ul from reagent reservoirs 4a amp b or from Sample Plate into DynaChip strip wells 2 This part picks up pipette tips out of the tip racks 3a amp b Used tips will be discarded into the waste bag 9 This part also moves the DynaChip ArrayStrip Frame into position for reading 7 2 Wash 5b Washing of chips with liquids from external buffer utilising the aspirating and dispensing channels during defined time intervals The washing buffer is dispensed from the external wash container The removed liquid is discarded into external waste container with aspirator channel Fig 3 Liquid Handling Head Wash part 5b allows simultaneous aspirating and dispensing of solutions Pipetting part 5a runs pipetting steps 1 5 Additional DynaChip Processor functions For several DynaChip Processor processing steps the Sample Plate needs to be moved in XY directions during these steps the Pipetting part of the Liquid Handling Head Fig 2 5a without applied tip is docking into the magnetic holder of the ArrayStrip Frame figure below enabling the XY movement for the following functions For array image detection at the end of a test each well of the ArrayStrip Frame is moved into reading position 7 see Fig 2
9. dry NOTE The tubing entering waste container must stay inside the container to collect remaining liquids For rinsing after longer periods of a non operating DynaChip Processor or before changing assays see also paragraph 5 1 13 4 2 Cooling System The DynaChip Processor is provided with a separate Cooling System enabling the cooling of the Reagent Reservoir at position 4a Cooling Fluid The cooling system generally does not reguire any maintenance However the level of the cooling fluid needs to be checked regularly In case the level inside the filler neck is below 5 mm additional cooling fluid needs to be added as described in the figure below Fig 20 For filling the cooling fluid remove black plug of the filler neck protruding from the cooling unit see also fig 6 Carefully fill with fluid using a syringe or pipette until 3 4 of the visible part of the filler neck is full Add fluid slowly to avoid foam formation After finishing close neck with plug NOTE Please use only cooling fluid specified by Supplier 4 3 Cleaning of Detection Window Check detection window regularly for contamination with liquid or dust particles For reliable array imaging the window must be free of any lint or liquid film Carefully clean soiled window using a soft cloth and water Avoid touching the window with any sharp edged object 4 4 Replacing Fuses If replacement of one or more fuses is required use only fuses
10. failure may be due to inappropriate wash buffer volume Ensure the user follow the DynaChip IFU 15 6 Appendix The DynaChip Processor is designed to be used in laboratories laboratory environment Only trained and authorised personnel are permitted to use the DynaChip Processor The Supplier is not responsible for any injury or damage caused by improper use of the DynaChip Processor Please read the following chapter carefully to avoid any misuse 6 1 Safety Information The DynaChip Processor was developed considering EU standards EN 55011 EN 61000 6 2 EN 61010 1 It complies with the following standards of the EU 89 336 EWG and 73 23 EWG Please observe the following caution labels located on the DynaChip Processor Plate at rear of instrument Disconnect mains before opening any closed part or casing of the instrument Warning sign on DynaChip Processor Lid Consider all safety notes when opening DynaChip Processor Lid Risk of injury by bruising Load instrument only with components and consumables specified in appendix 5 3 and specified by your Supplier Do not insert any other plates than ArrayStrip Frame and microtitreplates authorised by SUPPLIER Do not use any flammable explosive or corrosive material in combination with the device or its components WARNING Device is working using electrical current Do not open instrument casing or modify outside of descriptions in manual T
11. he Reagent Reservoir 4a of the instrument a second power supply is required Insert plug fixed of Power Connector e Fig 5 into the appropriate connector of the instrument d Fig 5 then connect this unit with a second power supply cable into power socket WARNING The power supply for the Cooling Unit must be connected only to power sockets with an operating voltage of 90 264 V AC at 47 63 Hz e Place computer next to the DynaChip Processor instrument Connect appropriate power supply cable of computer with the related line connectors and plug them into a power socket e For communication between DynaChip Processor and computer plug both camera cable b Fig 6 or Fig 7 and serial cable c Fig 6 or Fig 7 into the appropriate port inlets of DynaChip Processor instrument Plug these cables into appropriate connectors of the computer Fig 7 plug in camera cable camera adapter and serial control cable COM adapter 2 2 Rack Installations For delivery the rack holding Sample Plate and Tip Racks and the two Reagent Reservoir Racks are removed and packed separately These need to be placed into the DynaChip Processor after transportation see Fig 8 below Fig 8 View into DynaChip Processor after removal of transportation interlocks with installed ArrayStrip Frame 2 and Incubator Unit 6 At the rear part the positions for the Reagent Reservoir Racks 4 can be seen in the front part the metal pins
12. he application of other hazardous or infectious material is in full responsibility of the user WARNING During instrument operation do not open Lid and do not perform any operations inside DynaChip Processor working area RISK of bruising CAUTION Do not touch incubator unit Hot surfaces NOTE Only devices cleared with a decontamination certificate as attached on the end of this manual will be accepted for inspection and service Electrical Power The DynaChip Processor is licensed for an operating voltage of 85 230 V at 50 60 Hz Do not run instrument at any other operating voltage The external Cooling Unit is licensed for an operating voltage of 90 264 V at 47 63 Hz Do not run instrument at any other operating voltage Connect instrument and external Cooling Unit only with the provided power supply cables to a power socket with a protective conductor earth ground Apply only cables and connectors without any defect and which are not twisted 16 The right Environment Before unpacking the reader ensure that the temperature of the reader has reached room temperature in order to avoid condensation collecting within the reader Allow temperature eguilibration for at least 6 hours The instrument was designed for use in a laboratory environment Keep free of dust harsh and explosive solvents and acidic vapours Protect the instrument against humidity gt 85 Avoid direct sunlight and vibration Ensure free ventilation of
13. hips 8 well strips for inserting into ArrayStrip Frame SUPPLIER Sample Plates 96 well microplates tested and authorised by Supplier NUNC U96 PP 0 5 ml catalogue no 267245 please contact Supplier for plates from other Suppliers DynaChip Processor Pipetting Tips 250 ul nominal volume capacity of 96 tips rack CyBio AG Germany cat no OL 2001 25 300 IVGN Cat 889 01D Tubings for external Reservoirs and Waste Container Silicone tubing with 01 6 03 2mm inner outer diameter 120 mm in length Novodirect D71558 Cooling Fluid cooling fluid based on propylene local Suppliers glycol 30 with corrosion inhibitor Fuses Delay fuse 5x20 mm 10A local Suppliers 6 4 Warranty Disclaimer SUPPLIER warrants that the supplied products meet the specifications contained in the technical data sheets and the specifications stated in the manuals SUPPLIER warrants that its products are of good quality and suitable for normal use SUPPLIER s obligation and the purchaser s exclusive remedy under this warranty is limited either to replacement at SUPPLIER s expense of any products which shall be defective in manufacture and which shall be returned to SUPPLIER transportation prepaid or at SUPPLIER s option refund of the purchase price Claims for merchandise damaged in transit must be submitted to the carrier This warranty shall not apply to any products which shall have been altered outs
14. ide SUPPLIER nor shall it apply to any products which have been subjected to misuse or mishandling A Warranty for a period of one year is valid for all parts besides wear and tear parts and consumables ALL OTHER WARRANTIES EXPRESSED IMPLIED OR STATUTORY ARE HEREBY SPECIFICALLY EXCLUDED IN NO EVENT SHALL SUPPLIER BE LIABLE FOR ANY SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES 19 Decontamination Certificate Institute Name amp Address Instrument Serial No It has not been used in an invasive procedure The instrument has been cleaned and decontaminated in preparation for inspection service or repair ial This instrument has not been in contact with blood other body fluids or any infectious sample material The decontamination procedure is as outlined below The instrument could be contaminated The nature of risk and safety precautions to be adopted are as follows Signed Position Address Tel No 20 CONTACT DETAILS For country specific contact information visit our web site at www invitrogen com 11 Bassendale Road Croft Business Park Bromborough Wirral CH62 3GL U K Tel 44 151 346 1234 Fax 44 151 346 1223 e mail eurotech invitrogen com Copyright 2006 Dynal Biotech Ltd U K All rights reserved Printed 010 08 06 Rev 000
15. invitrogen DYNAL Invitrogen Corporation DynaChip Processor User Manual Rev 002 Table of Contents 1 7 IntroductiOr eii A dw SN WI FU ds GR FN AGA dn Fd AGM Flaidd di dn Mdd iddod 3 1 1 System OvervIew RT TER RES NR RR HN RR CN Y aden 3 1 2 DynacChip Processor Componentbn aiii 4 1 3 Schematic Overview of main DynaChip Processor Component Positions oocooconincononnnnonnnocncnoranaononooo 5 1 4 liquid Handling Head E eine Saati Ne a dl 5 1 5 Additional DynaChip Processor FUNCTIONS ccccccseseseescscscsssesesececscssssesesscescsesessssesescecsesessssesescecsesesseaeeececseseeaeses 6 2 Installati n ee iad ca cesseuch secede GYFF dodd snes ends E E RR E a R E e a 6 2 1 Instrument Unpacking amp SQtup cc cccccccsccssscscssesesesssscsessscesesssscscssssesessssssesssssescsescessssssescsssscssssesesceecseseseseseeesee 6 2 2 Rack Installations sarte 8 2 2 1 Rack holding Sample Plate and Tip RackS rosrsvsnavevrsvonrnrrvevevrsvenerrrerevesvenesrrverenssnenesrsverensrvenesrrnenenssvenesssserennsnen 9 2 2 2 RESENOIrR ks a eM A nd NE 9 2 3 Tube Connecting ua 10 2 3 1 External Buffer Bottles dl 8 amp 2 coord DDUG haw dak ana nanan 10 2 3 2 Waste Contalner ass 10 2 4 DynaGhip Processor da tele RA ee i ee RS 11 2 5 Communication TOS ici diia 11 A A O 13 3 1 TUD O o ry dd 13 3 2 Tip Racks and Tip Waste Bag c cccccccccscsesescsssseseseescscsesssesesessesesss
16. of the type and rating specified in appendix 6 3 The fuses are situated close to the power plug next to the red ON OFF switch To replace fuses open black lidded plate remove old fuses and carefully insert new ones ATTENTION Before opening lidded plate and replacing fuses disconnect power supply Fig 21 Push back spring of fuse holder by applying a small screwdriver Holder and fuse will then be automatically released and can be removed for replacing with new fuse 14 5 Trouble shooting If you encounter difficulties with the ASP instrument the following trouble shooting list may help to resolve them If a solution could not be found the gained information from these observations will help qualified service engineers to specify the appeared failure function and to solve the problem properly Error Description Solution ASP does not start Ensure that instrument and control computer are properly connected and turned ON Check if green status LED on the front part is ON If not fuses might have to be replaced refer to paragraph 4 4 After turning ON ASP instrument Ensure that instrument are properly connected and turned ON green Statis ED IS PORON Check fuses If necessary replace them refer to paragraph 4 4 No communication between the A dialog box will be displayed giving the options Abort retry or ignore computer and instrument If the user select ignore the software can be used independently of
17. ontinue if not the run are aborted For loading of DynaChip Processor the opened Lid can be fixed into the grooves on the upper frame of the instrument Fig 13 Lid Illustration shows how the opened Lid can be fixed into the grooves on the upper frame of the instrument 2 5 Communication Test The communication test is part of the DynaChip Processor module in the DynaChip software The communication test is performed when the user launches the processor Before launching the processor ensure that the Lid is closed Fig 1 and that the green LED light at the front part of the DynaChip Processor is lit indicating the instrument has power and is ready for operation Fig 15 If there are no communication between the computer and instrument then a dialog box will be displayed giving the options Abort retry or ignore Once communication test is completed an automated hardware calibration process will start during which the Liquid Handling Head moves into several defined XY positions for adjustment After calibration is completed the unit moves into its starting position at the right hand side rear corner of the XY stage NOTE Start DynaChip Software only after switching on DynaChip Processor CAUTION To immediately abort the DynaChip Processor push the red STOP button Fig 15 NOTE The STOP button should only be used for emergency stops this is not the power button 11 Fig 14 Liguid Handling Head in St
18. rious injury 1 Introduction The DynaChip Processor is designed for the automated performance of multiplex tests in the DynaChip strip which are standard 8 well strips with a protein array chip integrated in the bottom of each well Up to 12 DynaChip strips can be placed into the DynaChip Processor for the parallel multi parameter testing of up to 96 individual samples The DynaChip Processor itself is a liquid handling machine with an integrated detection unit performing all liguid handling and incubation steps of an assay including array image detection and analysis Automated device control and array image analysis is done via the DynaChip software installed on an external computer connected to the DynaChip Processor 1 1 System Overview Fig 1 Overview DynaChip Processor and Computer 1 2 DynaChip Processor Components Sample plate 1 Array Strip Frame with DynaChip strips 2 Tip Racks 3a amp b Reagent Reservoir Racks with Reagent Bottles 4a amp b Liquid Handling Head 5 Incubation Unit not used in this instrument version 6 and Reading Position 7 Wash Fountain not in use in this instrument version 8 Tip Waste bag 9 Fig 2 Detailed insight view of all DynaChip Processor components for specifications see table below position name function 1 Sample plate Position of Sample plate containing all samples Capacity 1 96 samples 2 ArraySt
19. rip Frame Aluminium microplate frame for insertion of DynaChip strip Capacity 1 12 DynaChip strip Note Use only ArrayStrip frame provided with the Processor 3 Tip Racks Racks 3a and 3b containing DynaChip Processor pipetting tips Cat 889 01D Capacity up to 96 tips rack When processing 1 11 strips load one rack in position 1 if processing a full 12 strip run a second rack is required Note Use only specified tips with the DynaChip Processor 4 Reagent Reservoir Racks Two racks at positions 4a and 4b each for the insertion of up to 6 reservoir bottles for reagent solutions Capacity 2 x 6 containments for bottles of 20 ml nominal volume Note position 4a allows cooling of the inserted rack Note Insert only reservoir bottles provided by Supplier 5 Liquid Handling Head _ Central Liquid Handling Head for all dispensing aspirating and pipetting steps _ The unit is connected to external wash buffer reservoir and to a waste container 6 Incubation Unit Not in use 7 Reading Position _ Position for serial array image acquisition at the end of each test 8 Wash Fountain Not in use 9 Tip Waste Container for used tips 1 3 Schematic Overview of main DynaChip Processor Component Positions 2 Magazi G Ho Maga A 3 PE EOS 3a 1 3b 1 4 Liquid Handling Head The Liquid Handling Head consists of the wash unit combine
20. s Liquid Handling Head XY Unit with separate DynaChip Processor Aspirator and Dispenser channel and Pipetting part Incubation Unit thermoelectric heating Detection Unit pixel resolution 752 x582 Camera Interface camera SUB D 15 pole Computer Interface RS232 C SUB D 9 pole Computer Processor Disk Space 160 GB IDE RAM at least 512 MB Operating System MS Windows XP Pro Environmental Operating Temperature OT 15 35 C 59 95 F Incubator Temperature OT 70 C OT 158 F Cooling Unit minimum temperature 10 C Storage Temperature 10 50 C Relative Humidity no relative humidity leading to condensation within device Sunlight No direct sunlight Method of Disposal at the responsibility of the Supplier s customer Usage commercial Altitude up to 2000 m Power DynaChip Processor Supply 85 230 V AC 50 60 Hz Consumption 375 W T10A Fuses Delay fuse 5x20 mm 10 A Power Cooling Unit Supply 100 240 V AC 47 63 Hz Consumption 65 W max Physical Dimensions width x depth x height 588 x 770 x 563 mm height with opened lid 970 mm Weight approx 30 kg 18 6 3 Accessories and Disposables Accessory Specifications Supplier DynaChip Processor Reservoir Bottles 20 ml nominal volume Only bottles provided by Supplier DynaC
21. secure it with the provided fixing notches Carefully remove interlocks as described in the following figure Carefully unplug red rubber interlock positioned at the top of the Liguid Handling Head Remove red styrofoam blocks Fig 5 DynaChip Processor after delivery with transportation interlocks In case the DynaChip Processor needs to be shipped back please insert the interlocks before moving the instrument During transportation avoid any strong impacts which may lead to alteration of both Liquid Handling Head and Optical Unit position Connecting Tubing and Electricity All electrical and tubing connectors are situated at the rear of the instrument Fig 6 Fig 6 rear view of DynaChip Processor with power connector and ON OFF switch a Camera Cable connector b RS232 Cable connector c connection for Cooling Unit of Reagent Reservoir Rack d and Power Connector for Cooling Unit of Reagent Reservoir Rack e input output connectors for Waste and external Buffer Reservoir tubing f e Connect the provided power supply cable with the instrument s line connector at the rear a Fig 5 and plug it into a power socket Ensure that your voltage corresponds to the operating voltage of 85 230 V AC at 50 60 Hz which is required for the DynaChip Processor WARNING The instrument must only be connected to power sockets with an operating voltage of 85 230 V AC at 50 60 Hz e To run the Cooling Unit for t
22. sesesesessesssseassesessessssesesesecsesasseacsececsesacseseeeeecsenees 13 3 3 Cooling UNE secs isc fs ed eee EG 13 4 DynaChip Processor Maintenance sesesesesesessssnsnensssssersrensnsnenenesenensesesensnenensssssnenenensssssensnsnenenenesenensesesenenenenessssnenenee 13 4 1 TUG RIMSING are 13 4 2 Cooling System ee rain ii tii TD GYN 14 4 3 Cleaning of Detection Window ccccccecesesesssssesesesesesesesesesesssssessscscnsssesesesesesesesesesesesecececaesesenensasscsenseseseseasasieeeeeseees 14 4 4 Replacing FUSES oi I beatin ae dee dew 14 5 Trouble SACO NI ae YFC TORF cn A AF UEF NU YI OO Fyn 15 O O NRO 16 6 1 Safety Information iii a Y Ddr iio 16 6 2 Technical Details A a 18 6 3 Accessories and Disposables ccccccccsssesescssssesessescscsessssesssesecsesessesesesescsessssesssesessesssseassesecsesasssaseeceesesecseaeeeeecsenees 19 6 4 Warranty Disclaimer sica a aii 19 Decontamination Certificate ooeosesecesessosscseoscoscesesecosessoscesesscosessoscosesscsseseoscosesscseesesscosessossesesseosessoscesessesseseoseesesseseesssse 20 Definition of User Attention Words NOTE Indicates information that is necessary for proper instrument operation CAUTION Indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices WARNING Indicates a potentially hazardous situation which if not avoided may result in death or in se
23. should have a volume capacity of at least 1 e g 11 glass bottle Place tubing in Bottle to ensure continuous availability of liquid 2 3 2 Waste Container To dissipate the liquids of the DynaChip Processor aspiration steps the Liquid Handling Head needs to be connected to an external Waste container e Connect one end of provided tubing to the outlet OUT on the rear side of the DynaChip Processor fig 12 e Connect other end of tubing into appropriate empty waste bottle s The bottle should have a volume capacity of at least 1 I e g 1 I glass bottle NOTE During automated processing of 12 ArrayStrips 96 samples in one run typical volumes of DynaChip Processor aspirated and discarded liquid is 0 5 11 NOTE Before beginning a new run ensure that waste container is emptied 10 2 4 DynaChip Processor Lid Always close Lid before starting any processing program Close Lid before launching the controlling software from the DynaChip Software because after launch the Liquid Handling Head will move as part of an initialisation procedure ATTENTION Risk of Injury CAUTION Do not open Lid during DynaChip Processor run An alarm will sound if lid are opened during a run Should the Lid be opened during test procedure all mechanical steps will automatically be interrupted which may lead to incorrect testing and test results If the Lid is closed within 5 minutes 300 seconds of opening the run will c
Download Pdf Manuals
Related Search