3 Cleaning & Maintaining the MagPurix 12 system
Contents
1. and humidity is 30 to 8096RH non condensing A location that is flat and stable with no vibration A location away from direct sunlight Block the sunlight by closing curtains or blinds as necessary A location which is well ventilated and not dusty A location where the temperature does not go up and down www ZINEXTS com Page 10 Ver V1 00 suddenly Warming a cold room suddenly or moving the MagPurix 12 system from a room with low temperature to a warm room may cause condensation inside the device resulting in abnormal extraction B A location where the temperature and humidity are kept within the specified range far from water taps water heaters humidifiers air conditioners and heaters m A location far from objects which generate strong magnetic fields motors transformers TV audio speakers magnets etc Bringing the MagPurix 12 system close to any type of magnetic field may cause a malfunction AN Warning Do not use the MagPurix 12 system to a location where it is wet or can be splashed with water It may cause a device failure fire or electric shock When relocating the MagPurix 12 system disconnect the plug from the outlet first If the power cable is damaged this may cause a device failure fire injury or electric shock AN Caution Do not use the MagPurix 12 system in an unstable place such as a slanted surface or a place subject to vibrations It may cause injury or device failure Do no2. MagPurix 12 system Check that the following are included in the package Contact your local representative if you notice any missing part s ZZINEXTS MagPurix 12s Automatic Nuc Q ty A MagPurix Instrument 1 B Power Cord 1 C Sample Tube Rack 1 D Barcode Reader 1 E User s Manual 1 www ZINEXTS com Page 8 Ver V1 00 Please make allthe components are free from damages as soon as you get the system If any damage found please contact your local representative for the instant support Note Zinexts s global warranty does not cover the damages from the transportation or improper operation 1 2 Contents of Reagent Kits Reagent Cartridge s Reaction Chamber s Tip Holder Pestle essential for some Kit types Filtered tip Sample tube Elute tube ommoomw gt www ZINEXTS com Page 9 Ver V1 00 Note Reagent kits are separated purchase Please contact your local agents or representatives to get further info The contents of reagent kits will be various Refer to the handbook of kits enclosed in reagent box for details 1 3 Operating Environment Condition Use the MagPurix 12 system in a location that meets the following conditions B The space for the allocation of the MagPurix 12 system system must be remained 5 10 cm from instrument to side walls B A location where power can be provided Be sure to use the attached AC adapter B A location where the temperature is 15 to 30 C
3. Repairs to the MagPurix 12 system should only be performed by such agencies as are specifically authorized by the ZINEXTS LIFE SCIENCE CORPORATION Only original the ZINEXTS LIFE SCIENCE CORPORATION replacement parts should be used If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired About Reagent Kits N CAUTION Note When handling any of the kits refer to the respective handbook Reagents in each kit should be handled by observing the safety information and precautions regarding the kit Extraction should be performed in an appropriate laboratory or workplace The kits are not supplied with the MagPurix 12 system Select the desired kit s and order it them separately www ZINEXTS com Page 6 Ver V1 00 About Samples N BIOHAZARD B Always wear appropriate gloves a mask and safety goggles etc when handling any infectious samples About Infectious Wastes B When handling or disposing of infectious materials follow the laboratory guideline or the law regarding infectious waste to perform proper incineration fusion sterilization and or disinfection B When you use a third party to dispose of it outsource this work an operator licensed to handle medical waste subject to special control and give them the manifest of the medical waste at the same time www ZINEXTS com Page 7 Ver V1 00 1 Installation 1 1 Composition of the
4. expect 2 Remove the Cartridges and plastic disposables from the instrument and then dispose of them www ZINEXTS com Page 23 Ver V1 00 3 Cleaning amp Maintaining the MagPurix 12 system Two types of maintenance are performed on the the MagPurix 12 system Instrument as listed in the table For details on each type of maintenance see below Maintenance Type Performed by Schedule Routine Cleaning of Sample Tray After each use Cleaning of instrument Bi weekly body outside Cleaning of instrument Bi weekly body inside Service Engineer B Clean the Sample Tray with mild detergent and rinse with deionized water Allow the parts to dry before use Clean and disinfect the platform surface by wiping with deionized water followed by 70 ethanol B Clean the instrument body by removing dust gently with a dry soft cloth If the outside of the MagPurix 12 system is heavily soiled or if any samples that may cause infection are adhered to the outside of the the MagPurix 12 system wipe with a soft paper tissue soaked with 0 5 sodium hypochlorite solution or ethanol www ZINEXTS com Page 24 Ver V1 00 4 Troubleshooting Probie ease sown Instrument Problems No power the LCD Screen remains blank when the power is turned on LCD Screen turns on when the power is on but the self testing program does not run Protocol stops after an initial start Bubbles formed during extraction Presence of buffer
5. in the Cartridge Tray Leakage from Filtered Tips or uneven liquid handling between Filtered Tips Blockage of tips www ZINEXTS com AC power cord is not connected Forget to remove the packing stuff from instrument Cartridge s Plasticwares Reaction Chamber Tip Holder Filtered Tip Pestle Sample Tube Elute Tube incorrectly loaded on the the MagPurix 12 system Problem with motion sensors Missed adding sample or sample volume is lower than the recommended volume Motor movements may not be smooth incorrect placement of plasticware or leakage from tips Air leakage on the Filtered Tip Use the correct cords MagPurix Reagent Kits proper motor movements Check AC power cord connections at both ends or Turn off the instrument and remove the packing stuff Technical problem Contact your local representative or agent Turn off the power and then turn it on again to stop the program The system will move back to initial state Re load them according to the instructions shown in Note you could not resume the protocol after stop and you may lose your samples Turn off the power and remove all samples and plasticwares Contact your local representatives Be sure to add the sample to tubes prior to starting the protocol To ensure proper mixing of reagents in the tip and prevent bubble formation during mixing make sure the sample volume is at least the recommended volume list
6. MagPurix 12s Automated Nucleic Acid Purification System Instruction Manual UMO1001 www ZINEXTS com Page 1 Ver V1 00 TABLE OF CONTENTS Page INTRODUCTION see 3 SAFETY INFORMATION u uhodux ine EN uExhE a 4 1 0 INSTALLATION 2 8 1 1 Composition of the MagPurix 12 System 8 1 2 Contents of Reagent Kits eeeeeeeeeeseeeeeeeeeenenn 9 1 3 Operating Environment Condition eeeeseeesssss 9 1 4 Unpack the MagPurix 12 System eeeeeeeeeeeses 11 Eo VS U VIEW P n 14 2 0 GETTING STARTED u u 15 2 1 Lurningtr the DOW CM nern 15 Bee FOO al lO A A EE E E EE 16 2o EXV ACUO 6 e rer 16 2 4 After Extraction Operation cccccccccsecccsececeeeeeeeeseeseeeseeeeseeeeas 23 3 0 Cleaning amp Maintaining the MagPurix 12 Instrument 24 40 TFOUDIESNOOUING Ee TEILT 25 5O SpeciCatlONS sonens ee ae 27 IAP PEIN DI oei see 28 www ZINEXTS com Page 2 Ver V1 00 INTRODUCTION Thank you for purchase of the MagPurix 12 system which is a fully automated standalone robot that can purify nucleic acids within 30 45 minutes With advanced magnetic bead separation technology it enables you to have high quality extraction results Moreover the most user friendly interface makes users free from troublesome parameter settings and maintenance This guide contains important information regard
7. S com Page 13 Ver V1 00 1 5 Overall View B Front view with Sliding Door closed LCD Screen Control Panel Sliding Door Moving Handler B Front view with Sliding Door opened UV Lamp Operational Area www ZINEXTS com Page 14 Ver V1 00 B Back View Power Switch Power Inlet 1 x Barcode Reader Port amp 1 x USB Port 2 Getting Started Biohazard Always wear appropriate gloves a mask and safety goggles during any biohazardous operations in extraction process Even when touching the device after any operation with a biohazard risk wear appropriate gloves and a mask since the device may be contaminated Important Before starting extraction put on appropriate gloves a mask and safety goggles if required by the operation In the operation from preparing samples to extraction completion be careful not to contaminate the samples with sweat saliva etc 2 1 Turning on the power www ZINEXTS com Page 15 Ver V1 00 1 Make sure that the power cable are connected securely to the the MagPurix 12 system 2 Connect the plug of the power cable to the outlet 2 2 Preparation The following preparations are required for extraction operation B liems to be prepared Gloves Mask Safety Goggles Reagent Cartridge Reaction Chamber Tip Holder Pestle Option Filtered tip Sample tube Elute tube www ZINEXTS com Page 16 Ver V1 00 B For the preparation of s
8. amples refer to the handbook of each kit Provided with the Reagent Kits 2 3 Extraction 1 Turn the power switch on the system will process self testing and then go to steady mode Note The system will block main functions before the completion of self testing process 2 Open the sliding door and remove the sample rack from the instrument www ZINEXTS com Page 17 Ver V1 00 3 Take out Reaction Cartridges and all plastics disposables Reaction Chamber Tip Holder Filtered Tip Pestle Sample Tube Elute Tube from the Reagent Kit box You need one Reaction Cartridge and one set of plastic plastic disposables per sample www ZINEXTS com Page 18 Ver V1 00 4 Place reagent cartridges Important Cartridge should be placed in a flat and stable table when setting up the Reagent Cartridge Set Cartridges in the order of the number from left to right Make sure that Cartridges are inserted in to the Cartridge Tray tightly You will hear a click if the cartridge is placed correctly on the tray You can load 1 12 cartridges on the tray depending on the number of samples that you wish to process 5 Place Reaction Chamber s 6 Place Tip Holder s www ZINEXTS com Page 19 Ver V1 00 MXN S E C um LS FEKKSA memet i ne CS 9 Place Sample Tube and Elute Tube to Sample Rack on the bench www ZINEXTS com Page 20 Ver V1 00 10 Load the sampl
9. e s to Sample Tube Note Pretreatments are essential for some sample types before loading to Sample Tube Please refer to the handbook of reagent kits for details Make sure the caps of Elute Tube are open as the figure shown above www ZINEXTS com Page 21 Ver V1 00 11 Place Sample Rack on the instrument platform Note Use two hands to handle the Sample Tray Make sure the Sample Tray be placed correctly in the instrument 12 Close the Sliding Door 13 Scan the protocol barcodes to select purification protocol sample volume and elute volume Note There is one protocol barcode paper enclosed in the reagent kit box Protocol s name sample volume and elution volume will be shown on LCD screen after protocol barcode is scanned 14 Follow the instructions displayed on LCD screen to double check the operating steps being completed before program running 15 Push Enter to confirm Instrument will start to run the protocol program automatically until whole processes are completed Note www ZINEXTS com Page 22 Ver V1 00 Ittakes from 30 to 45 minutes to complete the extraction according to reagent types Find the detailed time consumption by checking the handbook of your reagent Kits 2 4 After Extraction Operation 1 Take out the Elute Tubes The extraction yields in them Note You can apply quality checking or do downstream study or storage them as what you
10. ed in the handbook supplied with the Perform preventive maintenance annually to ensure Swap the air leaked Tip with new one Too much starting Decrease the amount of starting material Use the Page 25 Ver V 1 00 material causing recommended amount of starting material as listed in clumps or aggregates the Reagent Kit manual Handbook DNA Quality Problems Decrease the amount of starting material used Be sure to add Proteinase K during lysis if included in the Incomplete lysis protocol Make sure that the sample is completely immersed in the Lysis Buffer Be sure to process sample immediately after collection or Poor quality of starting store the sample at appropriate temperature The yield and materia Low DNA yield the quality of DNA isolated depend on the starting material During shipping some magnetic bead solution may adhere Insufficient amount of to the sealing foil of the cartridge To collect any bead magnetic beads added solution from the foil tap the cartridge to deposit the bead solution at the bottom of the well Ensure that the lysate does not contain any particulate Clogged Tips resulting material that can clog the tip sprout If needed centrifuge in DNA loss ES the sample prior to the MagPurix purification otore cartridge containing the beads at room temperature Do not freeze the cartridge as the beads may be irreparably No DNA Magnetic beads stored damaged recovered or handled improper
11. ing the safe use of the MagPurix 12 system Please read this manual carefully before you start to run the system at the first time especially for Safety Information If there is any question about how to install or operate it please contact our certified distributors agents or email to our technical support center support zinexts com MagPurix Magnetic Bead Purification Process www ZINEXTS com Page 3 Ver V1 00 SAFFETY INFORMATION The meanings of safety precaution marks are as follows N WARNING WARNING indicates a dangerous condition that may lead to death or serious injury N BIOHAZARD BIOHAZARD indicates exposure to a biological agent or condition that may be infectious or harmful N CAUTION CAUTION indicates the possibility of danger that may lead to minor or moderate personal injury CAUTION may also indicate an accident of property damage Important Important shows the important notes for usage as well as prohibited actions Note Note indicates the notes procedures that should be obeyed and Supplementary information for use For your safety and that of others follow the guidelines provided in the following pages concerning the use of the MagPurix 12 system www ZINEXTS com Page 4 Ver V1 00 About Instrument N WARNING Ignoring the following notations may lead to fire or electric shock In countries other than Taiwan US and Canada use a power cable that mee
12. ly Make sure that the beads are in solution at all times and do not dry Dried beads are non functional Remove any magnetic beads using a magnetic separator or Magnetic beads present Eluate centrifuge the sample in a microcentrifuge for 1 minute at in the eluate containing DNA maximum speed is discolored DNA contaminated with Minimize the amount of blood or blood stained sample used heme s 20 ul blood spot for forensics sample To prevent bubble formation during mixing make sure the Bubbles formed during gt sample volume is at least the recommended volume listed mixing steps in the manual supplied with Reagent Kits DNA is sheared Purified DNA Aliquot purified DNA and store at 4 C short term or 20 C or degraded repeatedly frozen and long term Avoid repeated freezing and thawing thawed DNA contaminated with Maintain a sterile environment while working i e wear DNases gloves and use DNase free reagents www ZINEXTS com Page 26 Ver V1 00 5 Specifications The MagPurix 12 system Was T 2777 Instrument Dimensions CE LV directive 73 23 EEC EMC directive 89 336 EEC EMC EN61326 1 Safety EN60101 1 www ZINEXTS com Page 27 Ver V1 00 APPENDIX B Replacement Optional Parts Barcode Reader A01001 Power Cord A01002 Filttered Tip A02001 Sample Tube A02002 Elute Tube A02003 B Product Guarantees The guaranty period shall be one year from the deliver
13. stem in its original shipping box or some other equivalent box www ZINEXTS com Page 29 Ver V1 00
14. t use the MagPurix 12 system in direct sunlight or close to a heating device It may shorten the life of the MagPurix 12 system or cause a trouble Operating Conditions ems Conditions Temperature C During operation 15 30 00 www ZINEXTS com Page 11 Ver V1 00 Max wet bulb During operation 29 non condensing temperature C During down time 29 non condensing Temperature gradient C hr 12 or less non condensing Humidity gradient RH day 30 or less non condensing Altitude operating m 1600 or less 1 4 Unpack the MagPurix 12 system 1 Open the packing box and take out the instrument and related accessories Important The MagPurix 12 system has a weight of more than 45 kgs It should be lift and moved by two persons Hold the moving handler of the instrument from two sides to move it out from the box B Do not hold the plastic outer covering B Do not hold the front panel B Do not hold the door AN Caution Improper handling of the movement of the MagPurix 12 system will lead to instrument damages www ZINEXTS com Page 12 Ver V1 00 2 For correct and safe use of MagPurix 12s install itin a location that is close to electrical outlet and has enough space for installation and operation Important Keep the shipping box They will be needed again when transporting the MagPurix 12 system 3 Connect Barcode Reader with the Instrument EU If www ZINEXT
15. ts your country s standard or contact your local distributor Use the AC adapter provided Do not use the MagPurix 12 system with voltage other than the voltage specified on the device Do not overload the power outlet with too many devices Do not use the MagPurix 12 system with a damaged power plug or a loose socket If there is dust on the prongs of the power plug or on the plug socket remove it with a dry cloth When you disconnect the plug from the outlet be sure to hold the power plug itself Do not pull the power cable For maintenance disconnect the power plug from the outlet Do not touch the power plug when you hear the crash of thunder Do not pour any liquid on the MagPurix 12 system Do not place any objects containing liquid on the MagPurix 12 system Doing so may cause a device failure fire or electric shock B Inthe event the device overheats starts to smoke or smells strange immediately unplug the power cable N CAUTION B Never attempt to remodel the MagPurix 12 system without the manufacturer s permission Doing so may cause fire or electric shock B Do not place or drop objects on the MagPurix 12 system Also refrain from bumping or knocking it as doing so may cause a failure or malfunction of the MagPurix 12 system www ZINEXTS com Page 5 Ver V1 00 If any liquid materials are left inside the device wipe it up a soft paper tissue etc Otherwise the MagPurix 12 system may be damaged
16. y of the product Any part which fails to function properly under normal use provided that all warnings and cautions in this manual are observed during the guarantee period will be repaired by the ZINEXTS LIFE SCIENCE CORPORATION free of charge f the problem is due to the causes listed below some charge may be applied for the repair even within the guarantee period B Any failures due to improper use or that are affected by other devices except for those approved by the ZINEXTS LIFE SCIENCE CORPORATION B Any malfunctions or damages during transportation or due to dropping of the product by a user www ZINEXTS com Page 28 Ver V1 00 B About Services Before you ask for device repairs refer to 4 Troubleshooting to check the problems If the problems cannot be solved contact your local representative or the relative customer support B Notes for Transporting Shipping the MagPurix 12 system When transporting shipping the MagPurix 12 system to a new location perform the following tasks Warning Before carrying the MagPurix 12 system disconnect the plug from the outlet If the power cable is damaged this may cause a device failure fire injury or electric shock When transporting shipping the MagPurix 12 system be sure to perform the following tasks Remove all attached parts Sample Rack Barcode Reader from the MagPurix 12 system After completing the above tasks pack the MagPurix 12 sy
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