Instruction Manual
Contents
1. cccsecccseeeeseeeseeeeeeeeeees 11 Zee OMEGN TE Sta Oe esa gat Er eee 11 2 2 1 Electrical power supply ccc cceccceeecseeeseeeeeeeeneeesaeeseueeseeeseeeseueeseeenaess 11 BR PACUMATLIC DOWEL SUDDIY scrcicruiescieraisralcratetaiercdarcdatataratesatetalcsatesteiaiesats 11 2 2 3 SWITCHING ON the UNIt ccceseeceseeeececeseeeesceeesceeesceeesceeesceeesoeeesoeees 11 2 2 4 PNEUMATIC switching ON ccccceecceeeeseeeceeeceeeeneeecaeeceueeaeeeseeeseueeneeenaees 12 23 aM CHINOS SOD seca EEEE 12 2 3 1 Electrical turn off sssssssnsnnenkekk tk kk ERE REE E REE REEL R REE R ERE R REEL E ERR REE 12 292 FPneumalc UNOM EEEE 12 2 3 3 Emergency stop cccccccccseccsseeceeeeeeeeneeeseeeseueeseeeceeeseueeaeeeseeeseueeneeenaess 13 3 Use Sample Changer cccccccccccssccccececceeeeeceecesseeeesseecesseecesseesesseeeesseeeesageess 14 3 1 Start use and stop of the Sample changer cccecceseeeeeseeeeeeeeeeeeeeeeseees 14 Stel Checking the liguid levels 22 oa le re ars seE 14 3 1 2 Inspection and replacement of the Capillary cccccsecesseeeeeeeeeeeneees 15 3 1 3 Loading or unloading samples into the Sample Changev 0 17 User manual Release AO Page 7 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 1 General information 1 1 Bio SAXS Sample Changer description sample Changer for Bio SAXS is a scientific instrument installed at the ID14 3 beam line of2. Bio SAXS SAMPLE CHANGER ID14 3 EMBL ESRF User manual EMBL ESRF Bio SAXS Sample Changer 20 08 2010 User manual Release AO Page 2 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 User manual User manual Release AO Page 3 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 User manual Release AO Page 4 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 Release Release Date Name Noe 02 18 2011 HUET Julien Create Document es ns ERE BEER User manual Release AO Page 5 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 User manual Release AO Page 6 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 Contents Geer mIn SE EET ER E ede 3 1 General ATOM QU ON erriren a Ea ETEEN TE IENNE EO EATE EATEN 8 1 1 Bio SAXS Sample Changer description W u sssssssseseueneee keen nn kk enn 8 TL DEUS ampe Epo ure WW ea E 8 1 1 2 SCU Sample Changeur Unit i eccccsceceeseeeesseeeesseeeesseeeseaeees 8 Ml FGC AC eene E E E 8 TLF CORTOS TAICE eoe E EAE EAS 8 L F e E i OEE ET 8 12 Handling qualified personnel c ccccccseccceseeceeeeceeeeceeeceueeseueesueesseeeseaees 8 To I eneee EEEE EE EIEN EE EEE EEE EEEE EE 9 2 Sample Changer installation and start UP cccccccccssseecseeeeseeeeesaeeeeseeeeesaeees 11 2 1 Sample Changer installation cccccccccccseceeseeeeeseeeeeseeeesseseesseeeeseeeeesaees 11 See Bio SAXS Sample Changer Maintenance Quide
3. YELLOW by the ESRF safety can be investigated using the Sample Changer however cannot be recuperated back into PCR tubes or micro plates After the loading of a yellow sample into the exposure capillary the sample can be only evacuated into the waste canister during the cleaning cycle The sample defined GREEN by the ESRF safety can be investigated using the Sample Changer and can be recuperated into PCR tubes or micro plates after its loading into the exposure capillary or evacuated into the waste canister during the cleaning cycle lf during the manipulations of the WASTE canister a contamination occurs vaporize the KORSOLEX on an absorbing paper spray and absorbing paper are available in the experimental hutch and clean the contaminated surfaces The gloves and absorbing paper have to be thrown away after use into the yellow trash labelled DASRI Dechet d Activit de Soin a Risque Infectieux User manual Release AO Page 10 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 2 Sample Changer installation and start up 2 1 Sample Changer installation gt See Bio SAXS Sample Changer Maintenance guide 2 2 Sample Changer start up 2 2 1 Electrical power supply Sample Changer is permanently connected into the three phases networks of ESRF 2 2 2 Pneumatic power supply Sample changer is connected to the 6 bars air pressure network The type connector is quick 8mm Festo 2 2 3 Switching on the unit First switch on
4. emergency stop switch one in front of the SCU and one on the back of the SCU To turn off electrical and pneumatic power supply push one of two emergency stop switch To restart the SC unlock the emergency stop switch turn the button and push POWER ON blue switch on electronic rack User manual Release AO Page 13 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 3 Use Sample Changer 3 1 Start use and stop of the Sample changer Checking liquid tanks levels gt 4 1 1 Pneumatic switching on if necessary I 2 4 Electrical switching on 3 2 3 Starting BioSaxsSC PC and application software BSSC if necessary inspection of the POD 4 1 2 Load sample and buffers into the Sample Changer gt 4 1 3 Sample analyzing Load new sample and buffer into the SC and analysing gt 4 1 3 Unloading samples from the Sample Changer gt 4 1 4 Stop the application software and the BioSaxsSC computer Turn off the electronic rack gt 3 1 Turn off the pneumatics if necessary I 3 2 3 1 1 Checking the liquid levels If filling or changing the canisters USE GLOVES AND SAFETY GLASSES both can be found in the hutch Inspection of theH20 end DETERGENT canisters If the levels are low STOP THE POWER SUPPLY of the SC and fill H2O can distilled water DETERGENT can 2 Hellmanex Il 10 Ethanol 88 distilled water For the filling process just unscrew the blue cap fill the can
5. 1 position the Sample Changer rotary switch placed into the beam line see following picture The two water baths are supplied User manual Release AO Page 11 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 In second time to switching on the machine use the 3 push buttons on the front of electronic rack amp gt Activate the MAIN SWITCH the switch green light is on Beckhoff PLC is turned on POWER ON et OFF light swich are on blue and red Activate the POWER ON switch motors and pneumatic are turn on if cover is closed 2 2 4 Pneumatic switching on lt Pressure switch valve Pressure ON OFF valve Pressure indicator Turn pressure ON OFF valve Pressure indicator must display 6 bars 0 2bars if necessary adjust the pressure with the pressure switch valve 2 3 Sample Changer stop 2 3 1 Electrical turn off To stop the Sample Changer use the electrical rack switch or the main rotary beam line switch Activate the POWER OFF switch motors and pneumatic are turn off Activate the MAIN SWITCH switch lights are off Beckhoff PLC is turned off just two water baths are supplied Activate rotary beam line switch and the machine is completely off 2 3 2 Pneumatic turn off Activate the Pressure ON OFF valve to turn off the pneumatic User manual Release AO Page 12 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 2 3 3 Emergency stop The Sample Changer has two
6. and close the cap again Inspection of the WASTE canister If the can ts full STOP THE POWER SUPPLY of the SC and unscrew the black cap see photo on next page pull out the level switch and the tube connection and wipe it with absorbent paper see photo next page put the level switch into a new WASTE can which contains 300ml of KORSOLEX Throw the absorbent paper into a yellow waste bin located in the hutch Carefully remove the full waste container to avoid spillage use the black cup of the new canister to close the full one bring the full waste can to a recycling point of the ESRF User manual Release AO Page 14 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 Inspection that there is no liquid in the safety tray If there is any liquid contact a member of the maintenance personal Containers holding fresh distilled water and cleaning solution should be available at the beam line If they do not contain enough water and ethanol can be obtained in the sample preparation laboratory near the beam line HELLMANEX II blue 11 bottle is available at the beam line 3 1 2 Inspection and replacement of the capillary changed by the LOCAL CONTACT of ID14 EHS or the qualified maintenance personnel The Sample Changer power supply must be cut i ATTENTION the exposure unit is under vacuum the capillary must be Inspect visually the capillary by using the application software If it is broken or di
7. e situated on the marble table one in front and second behind of the SCU They shut down the electric power supply as well as pneumatic of SC Sample Changer emergency stop button e The beam line emergency stop buttons are EMERGENCY situated on the walls of the experimental STOP hutch as shown on the photograph on right ARRET ATTENTION Pressing this button will shut FDR tere down electric power supply of the entire FE beam line ID14 i e all 4 end stations Beam line RE start up represents a complicated and time consuming task which can take several hours The beam line emergency stop button has to be used only in i the case of danger veg User manual Release AO Page 9 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 Chemical and biological risks The DETERGENT canister contains a solution of the HELMANEX II 2 which is a corrosive chemical The WASTE canister contains the KORSOLEX and the HELLAMNEX II in solution being both corrosives When manipulating these canisters the use of gloves and glasses is obligatory consult security sheets Information and security sheets of the HELLMANEX II can be found on www chem agilent com Library msds 8529EU French paf Information and security sheets of the KORSOLEX are on www bode ch com media sdatenblaetterbodefr korsolex_endo cleaner fr pdf The sample defined RED by the ESRF safety cannot be investigated using the sample Changer The sample defined
8. o be performed by following qualified personnel FELISAZ Franck Tel 7188 E mail felisaz embl fr FODINGER Lukas Tel 7951 E mail foding embl fr GIRAUD Thierry Tel 2829 E mail giraud esrt fr CASEROTO Hugo Tel 2953 E mail hugo caserotto esrt fr GOBBO Alexandre Tel 7949 E mail goobo embl fr HUET Julien Tel 7714 E mail huet embl fr VILLARD Cyril Tel 7875 E mail cvillard embl fr User manual Release AO Page 8 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 1 3 Security In general the user has to follow security rules valid at the ESRF site In particular those which concern following operations the electric link of a given apparatus with a certified and protected socket the respect of compressed air use rules etc Stickers are put on some elements of Sample Changer It is important for your security that you follow the instructions written on them they prevent from possible risks electric chemical optic It is forbidden to dismount the fix protection covers which protect Sample Changer device itself AND also its users The movable Plexiglas cover is provided with a contact which shut down the power of motors pumps and compressed air when this cover is not in the closed position During a normal use the Sample Changer can be powered ON and OFF as described in the 2 of this document However in the case of emergency there are two ways how to stop it immediately e Two emergency stop buttons ar
9. rty Put SEU at room temperature Stop the Sample Changer and close the application software Close the two isolation valves as well as the valve of the vacuum pump Checking SEU is at room pressure by mean of ESRF BioSaxs application User manual Release AO Page 15 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 Unscrew the tube coming from the capillary on the syringe side following picture Unscrew here Unscrew and pull out sec ae POD black stop piece Unscrew Unscrew tube close to the POD black stop piece and tube connector close to needle Pull out the POD by mean of specific tool clamp closed to SCU Change the POD Replace the POD orient the alignment pin upwards Screw the POD black stop piece and the tube Reconnect the tube coming from the capillary to the syringe Activate the vacuum by the ESRF BioSaxs application software Wait until the vacuum is correct Restart the Sample Changer and the application software Clean the capillary and tubing with a normal cleaning cycle Note If the vacuum does not reach the correct level the capillary is User manual Release AO Page 16 EMBL ESRF Bio SAXS Sample Changer 20 08 2010 leaking and must be replaced once again If the problem persists contact a member of the maintenance personnel 3 1 3 Loading or unloading samples into the Sample Changer On BSSC application software click on LOAD Co
10. the ESRF which allows to expose a liquid solution into an X ray beam in order to analyse it The robot has 5 parts SEU Sample Exposure Unit SCU Sample Changer Unit the fluidic rack the controller rack and a PC 1 1 1 SEU Sample Exposure Unit The Sample Exposure Unit contains a quartz capillary glued into an inox fix Support pod placed in X ray beam under vacuum in which the analysed liquid is transferred a camera and a lighting system to visualise the capillary The cell is thermally controlled between 2 and 60 C 1 1 2 SCU Sample Changeur Unit The Sample Changer Unit is able to displace sample plates along three axes Its cleaning system contains two wells one with detergent and water second well with dry air 1 1 3 Fluidic rack The fluidic rack ensures sample recuperation after its X ray exposition in the SEU capillary cleaning and temperature regulation in the SCU and SEU using two independent liquid circuits 1 1 4 Controller rack The controller rack provides the supply of various Sample Changer modules by electricity and compressed air SEU SCU Fluidic rack It contains also a programmable automate to operate different modules 1 1 5 PC PC standard equipped with the application SC_BS allowing to run Sample Changer 1 2 Handling qualified personnel The Sample Changer maintenance and handling has to be carried out by designed personnel All interventions as dismounting reparation and others have t
11. ver locked must become blue Open cover es gr N Gh K as Dor FED Remove if necessary adhesive tape and PCR tubes of the samples and buffers Put in the PCR tubes with your sample of interest an buffers or well plates Cover if necessary with adhesive tape Close the cover On BSSC application software click on Scan and Parked Cover locked must become green NOTE Use only compatible well plates PCR Ependorf tubes with the SC Contact local contact to obtain the different parts references Check that the samples do not contain bubbles before putting them into the sample Changer User manual Release AO Page 17
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