User Manual - Leica Microsystems
Contents
1. Contents Copyright 2 Function of the Microscopes 3 The Reliable Route to Success 26 General Notes 4 Overview of an M Series Microscope 27 Important Safety Notes 5 The Correct Interpupillary Distance 28 Symbols Used 6 Using the Eyepieces 29 Safety Instructions 7 Focusing 30 Adjusting the Resistance of the Focus Drive 31 Changing the Magnification Zoom 32 Congratulations 14 Click Stops and Magnification Levels 33 Maximum Compatibility 15 On We Go 16 Magnification Factor of the Eyepieces 35 Health Notes 36 Base and Focusing Column 18 Dioptric Correction 37 Microscope Carrier 19 Dioptric Correction and Parfocality 38 Optics Carrier 20 Graticules 39 Tube 21 Eyepieces 22 Objective 23 TL ST Transmitted Light Base for Leica M60 B and The Different Types of Objectives 41 TL5000 Ergo Transmitted Light Base for Leica M80 B 24 Leica M series User Manual 11 Bases Leica TL ST Transmitted Light Base Controls Leica M60 B 43 Leica TL ST Transmitted Light Base Operation 44 Leica TL ST Transmitted Light Base Lamp Replacement 45 Leica TL5000 Ergo Transmitted Light Base Controls Leica M80 B 46 Leica TL5000 Ergo Transmitted Light Base Operation 47 Specifications for the Bases Leica TL ST Transmitted Light Base 50 Leica TL5000 Ergo Transmitted Light Base 51 Appendix Calculating the Total Magnification and Field of View Diameter 53 Care Maintena2. Leica MICROSYSTEMS Living up to Life User Manual Leica M60 B Leica M80 B ji sg CE Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH Reproduction of text or illustrations in whole or in part by print photocopy microfilm or other method including electronic systems is not allowed without express written permission from Leica Microsystems CMS GmbH The instructions contained in the following documentation reflect state of the art tech nology We have compiled the texts and illus trations as accurately as possible Still we are always grateful for comments and suggestions regarding potential mistakes within this docu mentation The information included in this manual may be changed without prior notice Leica M series Revision 1 0 published March 14 2013 by Leica Microsystems Wetzlar GmbH Ernst Leitz StraBe 17 37 D 35578 Wetzlar Germany http www leica microsystems com Responsible for contents Marketing CMS User Manual 2 Function of the Microscopes The Leica M60 B and Leica M80 B microscopes for which this User Manual has been written are designed for routine examinations of cell and tissue cultures liquids and sediments This includes examining specimens taken from the human body for the purpose of gaining infor mation about physiological or pathological conditions or inborn anomalies or testing for safety and compatibility
3. Do not touch new halogen lamps with your bare fingers this drastically reduces the service life of the lamp Leica M series Changing lamps 1 Unscrew the two screws on the cooling unit and pull the cooling unit out along with the lamp 2 Carefully pull out the lamp and mount by pulling them upwards 3 Disconnect the lamp from the mount 4 Insert the new lamp into the mount and reinsert the lamp holder User Manual Safety precautions A When inserting the lamp ensure that the cables are inside the two metal clamps This prevents the cables from getting caught during insertion Leica TL5000 Ergo Transmitted Light Base Controls Leica M80 B 1 2 x d Rear side of the TL5000 Ergo Left side ofthe TL5000 Ergo Right side ofthe TL5000 Ergo 1 A Hu for the Le 1 si display for o ana Ae 1 On Off switch for the light source Press and 2 Power supply connection the aperture or or alance in DF mode hold for5 seconds tovesel the basoto factory 2 Control for the size of the aperture bal default settings nia 2 Controls for controlling the brightness inten 3 BF button for bright field press and si hold for 2 seconds to de activate the automatic aperture 4 RC button for Rottermann Contrast in the RC mode press and hold the button to define the aperture size 5 DF button for dark field Refer also to the separately provided RE O User Manual for the TL5000 Ergo trans mitte
4. for potential recipi ents or for monitoring therapeutic measures IVD The above named microscopes comply with the Council Directive 98 79 EC concerning in vitro diagnostics Leica M series The manufacturer assumes no liabil A ity for damage caused by or any risks arising from using the microscope for other purposes than those for which they are intended or not using them within the speci fications of Leica Microsystems CMS GmbH In such cases the Declaration of Conformity shall be invalid Leica M60 B nameplate Leica Leica MicrosystemsCMS GmbH Ernst Leitz Str 17 37 D 35578 Wetzlar al 1 2012 Model M60 8 VERO 04200000050 100 240V 60 60Hz max 30W A ivo Q Lamp 12V 20W Made in easpore User Manual These IVD instruments are not A intended for use in the patient environ ment defined by DIN VDE 0100 710 Nor are they designed to be combined with medical instruments in accordance with EN 60601 1 If a microscopeis electrically connected toa medical instrument in accordance with EN 60601 1 the requirements listed in EN 60601 1 1 shall apply Not suitable for examining potentially infec tious specimens This type of instrument may be operated by trained laboratory personnel only Leica M80 B nameplate Leica Leica MicrosystemsCMS GmbH Ernst Leitz Str 17 37 D 35578 Wetzlar 012 Model M80 B IL LKMAKELKANKKKA 33 V DC 130W max A Made in ngapere General Notes Use
5. in clean rooms The Leica M series can be used in clean rooms without any problems Cleaning Do not use any unsuitable cleaning agents chemicals or techniques for cleaning Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and products could be contaminated by abraded particles In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Leica M series The cleaning of glass surfaces and objec tives in particular should be carried out exclusively as outlined in the brochure Cleaning of Microscope Optics The infor mation can be downloaded at http www leica microsystems com prod ucts Select your product and go to the Down load page For additional information refer to page 55 User Manual Servicing k Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Responsibilities of person in charge of instrument Ensure that the Leica stereomicroscope is operated maintained and repaired by authorized and trained personnel only Important Safety Notes User Manual This User Manual describes the special func tions of the individual modules of the Leica M stereomicroscopy series and contains impor tant instructions for their operational safety
6. instruments and accessories described in this User Manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims as well as product liability and the Declaration of Conformity User Manual Place of use k Only use the instruments in enclosed dust free rooms and between 10 C and 40 C Protect the devices from oil chemi cals and extreme humidity If using the devices outdoors protect them from dust and moisture Never use electrical devices outdoors Electrical components must be placed at least 10 cm away from the wall and away from flammable substances Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort micrographic images for example In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Safety Instructions continued Responsibilities of person in charge of instrument These Safety Instructions must be available at the workplace Ensure that The M series of stereomicroscopes and accessories are operated maintained and repaired by authorized and trained person nel only All operators have r
7. maintenance and accessories You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the User Manual and the safety instructions from the supplier Leica M series Before installing operating or using the instru ments read the user manuals listed above In particular please follow all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals User Manual We guarantee the quality of our products Our guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Symbols Used Warning Safety hazard This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged instruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged instruments Leica M series Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor
8. to the highest level the specimen is always brought into sharp focus If not repeat the process Graticules Use Leica graticules make counting easier particu larly for workstations that are not equipped with a digital camera The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces 1 Screw the insert off of the eyepiece Leica M series ra Clamp the graticule on the insert applying moderate pressure Ensure that the grati cule fits tightly Screw the insert and graticule firmly into place and replace the eyepiece in the tube You can now align the graticule by rotating User Manual the eyepiece in the tube and then tighten ing it using the clamping screw Objectives and Optical Accessories The Different Types of Objectives To meet the various requirements regarding imaging properties there is a choice of high quality interchangeable planachromatic and planapochromatic objectives and also lower priced interchangeable achromatic objectives Achromatic objectives are particularly suited for three dimensional specimens with high contrast structures k Flat field planachromatic objectives are particularly well suited for studying flat objects such as wafers and thin sections With planapochromatic objectives the finest structures are visible with high contrast The sophisticated apochromatic correcti
9. alogen illumination 1 Switch on the illumination of the base at the power switch 3 Focuson the specimen 3 Set the illumination to the desired intensity using the left control Leica M series Control of the transmitted light The TL ST transmitted light base has a slider that automatically moves the path folding mirror in the base when moved The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light User Manual Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background k Move the slider backwards until the desired effect is achieved Inclined transmitted light Transmitted light that traverses the object obliquely will provide additional resolution and information when observing semitransparent opaque objects Slowly pull the slider towards yourself until the desired effect is achieved 44 Leica TL ST Transmitted Light Base Lamp Replacement Changing the halogen lamp Before you change the lamp it is abso lutely necessary to unplug the power plug from the base to prevent the risk of elec tric shock The halogen lamp becomes very hot during operation Therefore to avoid being burned let the base cool off for approx 10 minutes after switching it off A
10. d light base Leica M series User Manual 46 Leica TL5000 Ergo Transmitted Light Base Operation The transmitted light base must only be connected to a grounded socket with a faultless power cable Failure to observe these warnings may result in serious personal injury or even death The LED illumination can be very bright Check and adjust the intensity of the illumination to a suitable brightness before looking through the eyepieces Leica M series Switching the transmitted light base on and off 2 Press the on off switch on the right side 1 Switch on the transmitted light base with once to turn on the illumination the power switch on the rear side gt Continued on next page User Manual 47 Leica TL5000 Ergo Transmitted Light Base Operation Continued 3 Look through the eyepiece and adjust the H The following assumes that the power light intensity using the control on the switch on the rear side of the device right side The intensity of the illumination is always switched on This switch is not is visualized by the LED scale mentioned in the remaining part of this User Manual 4 Press the on off switch on the right side once again to turn off the illumination on the base Leica M series User Manual 48 Specifications for the Bases Leica TL ST Transmitted Light Base Light source Halogen lamp 12 V 20 W Quick illuminant change Yes Illuminat
11. ead understood and observe this User Manual and particularly the safety regulations Repairs service work Repairs may only be carried out by Leica Microsystems trained service technicians Before opening the instruments switch off the power and unplug the power cable Avoid contact with powered electrical circuits which can lead to injury tapon Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components In order to prevent damage from vibrations disassemble all moving parts that accord ing to the user manual can be assembled and disassembled by the customer and pack them separately AE cia in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for following all applicable safety instruc tions laws and guidelines ca Once the product has reached the end ofits service life please contact Leica Service or Sales about disposal Please observe and comply with the national and federal laws and regulations that are equivalent to EC directives such as WEEE Like all electronic devices the micro scope its accessory components and Only original Leica Microsystems spare parts may be used consumables must never be disposed of with general household waste User Manual 8 Leica M series Safety Instructions continu
12. eces and objectives with special optics cleaning cloths and pure alcohol Copyright Leica Microsystems CMS GmbH Ernst Leitz StraBe 35578 Wetzlar Germany 2013 Phone 49 6441 29 0 Fax 49 6441 29 2599 LEICA and the Leica logos are registered trademarks of Leica IR GmbH Printed on chlorine free bleached paper 111 13 M H Revision 1 0 issued March 14th 2013 CE www leica microsystems com eca MICROSYSTEMS
13. ed Legal regulations Observe the generally applicable statu tory and country specific regulations for accident prevention and environmental protection EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity See page 54 Health risks Workplaces with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the dura tion of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arrangement of workplace work Leica M series assignments and work flow changing tasks frequently Thorough training of the person nel giving consideration to ergonomic and organizational aspects The ergonomic design and construction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum Direct contact with eyepieces can be a potential transmission path for bacterial and viral infec tions of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups User Manual Car eful handling Exercise particular care when setting up the instruments If it is specified that two or more people are required for setup compli a
14. ed area 50mm Power supply Input voltage 100 240 V frequency 50 60 Hz Energy consumption 30 W max Ambient temperature 10 40 C Connections Power plug Weight 7 4 kg Illumination modes Bright field Yes Dark field Yes single sided Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control No Integrated filter holder Yes Coated optics for increasing the color Yes temperature Matching of high num aperture No Remote control options No AntiShock Pads Yes Dimensions WxHxD 340x430x85 mm Leica M series User Manual Leica TL5000 Ergo Transmitted Light Base Light source Light source LED Illuminated field Bright field 65 mm Illuminated field Dark field Y 40 mm Relief Contrast System RC Yes Internal shutter lamp control Yes Integrated filter holder Yes Matching of high num aperture Yes Remote control options Yes AntiShock Pads Yes Dimensions WxHxD 412x341x46 mm Power supply Input 100 240 VAC 47 63 Hz 3 2 A Output 33 VDC 3 93 A 130 W MAX Connections Power supply Leica M series User Manual Appendix Leica M series User Manual 52 Calculating the Total Magnification and Field of View Diameter NFOV Field number of the eyepiece Field numbers are print
15. ed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Example MO 1x objective ME 25x 9 5 eyepiece Parameter MO Magnification of objective ME Magnification of eyepiece Z Magnification changer position q Tube factor e g 1 5x for coaxial incident light 1 6x for 45 ErgoTube r Factor 1 25x if the planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 Calculation example magnification in the binocular tube MTOT VIS MOxMExzxqxr or 1x 25x 4x 1 5 x 1 25 187 5x Z Zoom position 4 q Coaxial incident light 1 5x tube factor r Factor 1 25x Calculation example field of view diameter in the specimen Leica M series User Manual 53 Care Maintenance Contact Persons We hope you enjoy using your high perfor mance microscope Leica microscopes are renowned for their robustness and long service life Observing the following care and clean ing tips will ensure that even after years and decades your Leica microscope will continue to work as well as it did on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica M series EC Declaration of Conformity To download the EC Declaration of Conformity use this link http www leica microsystems com products stereo microscopes macroscope
16. h dioptric correction and eyecups Fastening screws for the eyepieces Ergo tube Magnification changer zoom Optics carrier Interchangeable objective Transmitted light base Column 10 Coarse fine focusing The Correct Interpupillary Distance The interpupillary distance is correctly set if you Adjusting the interpupillary distance 3 Slowly approach the eyepieces with your see a single circular image field when looking 1 Look into the eyepieces eyes until you can see the complete image at a specimen field without corner cutting 2 Hold the eyepieces with both hands Push If you are still a novice microscope user you the eyepieces together or separate them may need a short time to become accustomed until you see a circular image to this Not to worry after a little while it will become automatic Reference values The distance between eye and eyepiece measures approx 22 mm for 10 23B wide field eyepieces for eyeglass wearers Leica M series User Manual 28 Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification Furthermore all Leica eyepieces can be equipped with practical grati cules that enable measuring and quantifying of specimens Dioptric correction A built in dioptric correctio
17. idually depending on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus ing Leica M series User Manual 31 Changing the Magnification Zoom All M series microscopes have an integrated Zooming zoom The name indicates the zoom range 1 Look into the eyepieces covered 2 Focus on the specimen Leica M60B Zoom 6 1 k Leica M80B Zoom 8 1 3 Rotate the magnification changer until the desired magnification is configured The rotary knob for the zoom can be used either left or right handed Leica M series User Manual Click Stops and Magnification Levels The zoom button can optionally be operated either with or without click stops Continu ous zoom is possible when the click stops are disabled which many users find convenient On the other hand when the click stops are enabled photographs measurement results etc can be reproduced more accurately Leica M series Enabling and disabling click stops 1 Push the button towards the knob to enable the click stops 2 Push the button away from the knob to disable the click stops User Manual Magnification and fields of view The formula on page 53 provides addi tional information about how to calculate the magnifications and field of view diameters with consideration given to the position of the magnificati
18. mation or explanations that are intended to provide clarity Explanatory notes This symbol within the text stands for addi tional information and explanations Figures 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Notes on how to dispose of the micro scope its components and expend China RoHS 50 year EFUP Environ ment Friendly Use Period IVD labeling nstrument for in vitro diagnostics wo IVD manufacturing date for example 11 2011 for MM YYYY November 2011 Safety Instructions Description t The individual modules fulfill the highest requirements for observation and docu mentation of Leica stereomicroscopes of the M series Intended use of instrument t Leica Microsystems microscopes are opti cal instruments for improving the visibility of objects or specimens through magnifi cation Accessories such as optical acces sories stands illumination cameras etc supplement the equipment configuration Non intended use Using the instrument in any way contrary to the specifications in the User Manual can lead to bodily harm and damage to objects Never use microscopes for in vivo examina tions or eye surgery if they are not expressly intended for such use Never install any other plug or unscrew optical systems Leica M series and mechanical parts unless expressly instructed to do so in the instructions The
19. n is available for eyeglass wearers For more information refer to page 37 Leica M series If you do not wear glasses 1 2 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise If an eyepiece is equipped with the inte If you wear glasses 1 Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear as otherwise the viewing distance is too great grated dioptric correction turn the value to the 0 mark User Manual 2 If an eyepiece is equipped with the inte grated dioptric correction turn the value to the 0 mark By the way one benefit of viewing with eyeglasses is a drastically lower risk of bacterial transmission see page 36 The soft material of the eyecup also ensures that your glasses will not be scratched even if they contact the eyepiece Focusing Focusing raises or lowers the stereomicroscope using the focusing drive The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective The focusing drive can be operated either left or right handed The coarse fine adjustment carries a load of up to 15 kg The resolution of the coarse fine adjust ment is 1 um Leica M series User Manual Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too loose or too tight Does the equipment tend to slide downwards The resistance can be adjusted indiv
20. nce Contact Persons 54 Leica M series User Manual 12 Leica M Series Leica M series User Manual 13 Congratulations The New Leica M Series Leica Microsystems introduces the Leica M60 B and M80 B two new high quality routine stereomicroscopes of the CMO product line from Leica Microsystems The optical brilliance and wide range of accessories make them the ideal stereomicroscopes for quality assurance and for in vitro diagnostics examinations The Leica M60 B and M80 B stereomicroscopes can be used for a wide range of routine applications with the 6 1 and 8 1 zoom and engaged precise click stops at predetermined magnifications The large working distance and brilliant imaging power show the finest details of your speci mens without losing the field of view over large specimens Leica M series Common to all three microscopes is the Leica range of accessories Whether the work requires a variety of illumination types a wide selec tion of objectives or the Leica swing arm system Leica Microsystems has a solution for everything Do you already own stereomicroscopic equipment and are thinking of switching to Leica No problem The Leica M60 B and M80 B fit into the same 76 mm diameter interface microscope carriers as with previous models and are therefore compatible with many suppliers They adapt easily to existing components and add the high quality imaging power of Leica stereomicroscopes to existing inspection
21. nce with this is mandatory Never spill any liquids on electrical instru ments This could cause the stereomicro scope and other equipment to become electrically live and damage people and instruments Never clean instruments using corro sive cleaning agents or those containing acetone For detailed information about care refer to the User Manual for the instru ment Check the power cables regularly Defective power cables can cause injuries Wait for bulbs to cool off before changing them Touching hot bulbs can cause burns Safety Instructions continued Light sources safety regulations External power supply for the TL5000 Ergo Light sources pose a potential irradia Leica M80 B tion risk glare UV radiation IR radiation Permitted power supply Therefore lamps have to be operated in SINPRO SPU130 110 closed housings and in installed condition Specifications k Never look directly into the beam path Input 100 240 V AC blinding hazard 47 63 Hz 3 2 A Do not select a white strongly reflective Output 33V DC background for the specimen 3 93 A max 130 W For indoor use only A Use only the power supply specified above Other power supplies must not be used If the original power supply fails or is damaged it must be replaced Repair is not permitted Original power supplies are avail able from your Leica branch office or Leica dealer Leica M series User Manual
22. on allows these objectives to attain the highest color brilliance and fidelity Leica M series Achromatic objectives The 0 32x 0 5x 0 63x 0 8x 1x 1 5x 2x achromatic objectives offer countless variants for selecting the object field diameter magnifi cation ranges and working distances see page 53 Planachromatic objective 1x For the highest requirements for overall image quality we recommend equipping the micro scope with the 1x plan flat field objective which returns sharp contrast rich object fields all the way to the border User Manual Achromatic objectives with a long focal length For special applications achromatic objectives with long working distances and focal lengths of f 100 mm to 400 mm are available 41 Leica M series User Manual 42 Leica TL ST Transmitted Light Base Controls Leica M60 B Refer also to the separately provided User Manual for the TL ST transmitted light base Extension plate of the Transmitted Light Base Rear side of the TL ST transmitted light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 3 Main power switch 1 Adapter plate for easy assembly of focusing drives 2 Removable glass plate Controller for light intensity 4 Adjustment for path folding mirror UY Leica M series User Manual 43 Leica TL ST Transmitted Light Base Operation Light intensity control The left control adjusts the intensity of the 12V 20W h
23. on changer and the eyepiece and objective combination used Eyepieces Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the micro scope but also has a critical effect on the maximum magnification The magnification factor is between 10x and 40x The following eyepieces are available for the M series Magnification Dioptric correction Order number 10x 5 diopter settings 10 450 023 16x 5 diopter settings 10 450 024 25x 5 diopter settings 10 450 025 40x 5 diopter settings 10 450 026 Leica M series User Manual 35 Health Notes Potential sources of infection Direct contact with eyepieces is a A potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partner TO LRI ad 43 f 4 tO por 39 Le e Gs i la VAL R La CA 1045 ae SCA PT a Separate eyecups are an effective way of prevent ing infections Leica M series User Manual 36 Dioptric Correction All Leica eyepieces are also available with built in dioptric correction allowing the microscope to be used without glasses even by those with vision problems The correction comprises 5 diopter settings Leica M series Using the dioptric correction 1 Set the dioptric correction of both e
24. pecimens from the stage plate first 2 Screw the objective clockwise into the optics carrier User Manual TL ST Transmitted Light Base for Leica M60 B and TL5000 Ergo Transmitted Light Base for Leica M80 B Unpacking the base The base is delivered with the adapter plate installed Make sure the instruments are unpacked on a flat sufficiently dimensioned and non slip surface Refer also to the separately provided User Manual for the TL ST transmitted light base and the TL5000 Ergo transmitted light base Tools used k Allen key 3 mm Leica M series Focusing drive and column Unscrew the extension plate from the base using the Allen key provided 1 User Manual Attach your focusing drive column to the bottom using the 4 Allen screws Reattach the adapter plate to its original position using the 6 Allen screws Quick Start Guide The Reliable Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner and naturally you want to get right to work This manual will familiarize you with the finer details of your micro scope The following pages contain important practical information that makes using it every day easier Take time to read it it s worth it Leica M series User Manual 26 Overview of an M Series Microscope 2 3 4 3 6 7 8 Leica M series User Manual Eyepieces for eyeglass wearers wit
25. processes User Manual 14 Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series like its predecessors remains compatible with existing series This means that objectives bases tubes and so on can be reused Objectives All objectives of this new generation have the same reference focal length as the objectives of the MZ generation If you prefer you can there fore continue to use the previous Leica objec tive series Leica M series Tubes The interface between the optics carrier and the tube has remained the same so exist ing tubes fit the new M series The new tubes are designed for eyepieces with field number 23 while the predecessor models were only designed for field number 21 resulting in a smaller object field Eyepieces The new M series eyepieces have an audible and tangible click to provide immediate feed back in case of accidental adjustment User Manual On We Go If your new Leica microscope has already If on the other hand you are assembling your been assembled and commissioned by your Leica microscope yourself continue with the Leica consultant click here to skip through Assembly chapter which begins on page the installation instructions and go directly 17 to the Quick Start Guide on page 25 Leica M series User Manual 16 Assembly Leica M series User Manual 17 Base and Focusing Column The first step is to connect the focu
26. s routine manual Select the microscope type and go to the Download page User Manual Care Protect your microscope from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or replaced unless doing so is specifically permitted and described in this manual Protect your microscope from oil and grease Do not grease guide surfaces or mechani cal parts Care Maintenance Contact Persons continued Protection from dirt Dust and dirt will affect the quality of your results k Puta dust cover over the microscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces k Keep accessories in a dust free place when not in use Leica M series Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuit able cleaning agents and techniques can damage polymers User Manual Permitted measures Clean the microscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety regulations Remove dust with a bellows and a soft paintbrush Clean eyepi
27. sing column Assembling the column adapter 2 Install the base on the column using the ofthe M series to the corresponding base 1 Remove the three screws provided from the three screws column Tools used Allen key 3 mm Tip Place the column on the edge of a table to attach the base and have another person help you Leica M series User Manual 18 Microscope Carrier Tool Alternative installation options Allen key provided Depending on the desired working distance the microscope carrier can be installed in the standard way or upside down see figure Assembly The microscope carrier is fastened to the column using the screw provided Leica M series User Manual 19 Optics Carrier Tools used None Leica M series Assembling the Optics Carrier 1 Unscrew the clamping screw on the holder for the optics carrier 2 Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of each other User Manual 2 Tighten the clamping screw below the Optics carrier Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner as is the IC80 HD camera module for the Leica M80 B Refer also to the separately provided User Manual for the IC80 HD camera module The images captured with the camera may not be used for diagnosis In this case an eval
28. uation with the eyepieces is required Tools used No tools required Leica M series Preparations 1 Unscrew the positioning screw and remove the protective cover Assembling the tube 2 Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove User Manual While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct posi tion Eyepieces Tools used Preparation Inserting the eyepieces No tools required 1 If you want to use an optional graticule 3 Push the eyepieces into the tubes as far as insert it now page 39 they will go and check to ensure that they Magnification range fit tightly and accurately You can extend the overall magnification range 2 Removethe plastic tube guard using available 10x 16x 25x and 40x wide field eyepieces for eyeglass wearers 4 Securely tighten the clamping screws Leica M series User Manual 22 Objective Tools used No tools required Preparation 1 Remove the protective cap on the optics carrier by turning it Leica M series Attaching the objective Hold the objective firmly during assem bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2x planapochromatic objective which is very heavy Remove all s
29. yepieces to the mid position 0 diopter settings While wearing your glasses look through the eyepieces and focus on the specimen Rotate both eyepieces to the maximum value of 5 Hold one eye closed and rotate the other eyepiece in direction until the specimen appears sharp Then open the other eye and correct the diopter settings until the image is uniformly sharp User Manual Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched The prerequisite for this is the correct setting of the diopters and the parfocality The following adjustments have to be carried out only once by each user Preparations Move the lever of the video phototube to the observation position and open the diaphragm Leica M series Adjusting 1 Set the dioptric correction for both eyepieces to 0 Select the lowest magnification and focus on a flat specimen Select the highest magnification and read just the sharpness Select the lowest magnification again but do not look into the eyepieces Rotate the eyepieces counterclockwise in the direction as far as they will go 5 diopter settings User Manual 7 Look into the eyepieces 8 Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Select the highest magnification and refo cus if necessary Now if you adjust the magnification from the lowest
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