Milliliter Flow M.P.C Modular Pumping Component User`s Manual
Contents
1. Component will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode When start ing the pump RATE mode will be the default mode To operate in Volume mode set a target volume and the pump will change modes to suit desired operation Smooth Flow New micro stepping pump profiles deliver very smooth and consistent flow CE Mark Approved The modular pump meets all relevant European safety requirements for laboratory equipment when used in accordance with guidlines outlined in this manual Publication 5415 002 REV B 2 Harvard Apparatus m Modular Pumping Component ml Modular Syringe Pump input connections RS 232 connection is thru 9 pin OTOR D Sub as shown CONNECTION JP4 ENCODER CONNECTION JP3 Defaults Settings Dia 4 61mm I1ml Address 00 UT POWER amp S232 CONNECTION JP1 tt tg JP1 1 24 VOLTS DC RETURN JP1 2 RUN STOP INPUT JP1 3 12V JP1 4 RUNNING OUTPUT JP1 5 24 VOLTS DC POSITIVE JP 1 6 RS232 GROUND JP1 7 RS232 REC DATA JP1 8 RS232 TRANS DATA JP 1 9 RS232 GROUND Rate 1 0ml hr Baud 9600 Mode RATE Packing List 1 Main Unit 2 6 ft motor encoder extension cable 3 6 ft RS 232 DC power extension cable 4 6 oz jar of lubricant Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component Installation Location Requirements for the Syringe Pump A sturdy level clean and dry su2. 0 35 23 80 60 26 60 140 38 40 Popper amp Sons Inc Perfektum Glass Size Diameter 0 25 cc 3 45 mm 0 5 3 45 1 4 50 2 8 92 3 8 99 5 11 70 10 14 70 20 19 58 30 22 70 50 29 00 100 35 70 Publication 5415 002 REV B Becton Dickinson Plastic Plasticpak Size Diameter 1 cc 4 78 mm 3 8 66 5 12 06 10 14 50 20 19 13 30 21 70 50 60 26 70 SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Air Tite All Plastic Size Diameter 2 5 CC 9 60 mm 5 0 12 45 10 15 90 20 20 05 30 22 50 50 29 00 Unimetrics Series 4000 amp 5000 Size Diameter 10 ul 0 46 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 Size Diameter 5 ul 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 25 23 0 50 32 6 Appendix C Flow Rates nominal syringe size Harvard Apparatus mIl Modular Pumping Component Publication 5415 002 REV B 5 Harvard Apparatus mIl Modular Pumping Component Appendix D Serial Communication Commands Queries and Responses After each transmission to the pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The
3. 80 RH Well Ventilated Room Altitude up to 2000 m DC Voltage Fluctuation not to Exceed 10 of Nominal Transient Overvoltage Category II Pump is Rated Pollution Degree 2 in Accordance with IEC 664 Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal Caution Pinch Hot Hazard Surface Caution ESD Sensitive Device Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component Introduction Theory of Operation The new ml Modular Pumping Component is designed as a low cost highly pre cise single syringe infusion pump module capable of low to moderate back pres sures The module includes a mechanism hardware and software and requires the addition of a power supply and user interface A dual ml Modular Pumping Component version can be produced upon customer request Typically the ml Modular Pumping Component holds one syringe of any make from 0 5 ul to 60ml The diameter of the syringe s is entered via your PC or other controller and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow This unit is designed to operate inside an appropriate ly rated fire electrical mechanical enclosure or cabinet The board may be removed for remote operation Features Two Modes of Operation Constant Flow Rate and Volume Dispense The ml Modular Pumping
4. Check Syringe Often The ml Modular Pumping Component will shut itself off when the syringe is empty or is otherwise overloaded Although this presents no hazard to the user or the pump it is prudent to check the syringe from time to time Maintenance Keep the ml Modular Pumping Component clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw It is important to keep the leadscrew clean and lubricated To clean the exterior surfaces above the baseplate use a lint free cloth to remove loose dust For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alcohol If the pump does not work properly contact Harvard Apparatus for appropri ate instructions Protecting Small Fragile Syringes The ml Modular Pumping Component will hold microliter size syringes down to 0 5pl size These small syringes have fine wire plungers that may be damaged if allowed to bottom out The ml Modular Pumping Component is equipped with an adjustable stop collar on one of the guide rods Use the provided hex wrench to position and lock the stop collar to prevent damage to small syringes Publication 5415 002 REV B s Harvard Apparatus mIl Modular Pumping Component HYPER TERMINAL Terminal Emulation Software Remote Control Via The RS 232 Interface Using HYPERTERMINAL Normally inclu
5. Milliliter Flow M P C Modular Pumping Component User s Manual MAI 70 2203 HARVARD APPARATUS Publication 5415 002 REV B Publication 5415 002 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its pro
6. a reasonable period of time for com pletion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If prac tical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and CAUTION is not for clinical use on human or veterinary FOR RESEARCH USE ONLY patients It is intended for research use only NOT FOR CLINICAL USE ON PATIENTS Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component General Safety Summary Please read the following safety precautions to ensure proper use of your modular syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Appropriate Power Supply Use only an SELV approved power supply Be sure to observe proper polarity of con nections when hooking up DC supply v
7. ded with most Windows operating systems ml Modular Pumping Component 1 Connect the Power RS 232 cable supplied between the ml Modular Pumping Component RS 232 IN port and a PC s serial port 2 On the PC running a Microsoft Windows Operating System select START PROGRAMS ACCESSORIES HYPERTERMINAL HYPERTERMINAL to start the Hyperterminal application If Hyperterminal is not available install it from the Microsoft Windows Operating System Install disks or CD ROM 3 Set up the appropriate COMPORT for the following re aa Bravenboer SS Configure ASCII Setup Baud Rate 9600 Echo ped characters locally Data Bits 8 Line delay 0 Parity None Character delay 0 Stop Bits 2 Wrap lines Flow Cntrl None Emulation Auto Detect Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component HYPER TERMINAL Terminal Emulation Software You may want to save the setup information under a descriptive filename 4 At the PC launch hyperterminal with the above setup specifications if it is not already running Type VER at the PC keyboard and verify that the pump module s version is displayed at the PC terminal 5 Type RUN to start the pump type STP to stop the pump After starting the pump 0 gt should be displayed indicating address 0 pump is infusing After stopping the pump 0 should be displayed Publication 5415 002 REV B 7 Harvard Apparatus mIl Modular Pumping Comp
8. ducts and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required Modular Pumping Component mI Harvard Apparatus Table of Contents SUBJECT PAGE NO General Information Warranty and Repairs 2 General Safety Summary cccsseeeeeeeeeeeeeeeeees 3 4 Introduction Theory of Operation iecec2ecigecaeiesecs nese sees sees 5 Features iii e tierce A E 5 Input Connections sfancsctsutannce Vehcecuasnanecteacnivetune 6 Packing LIS tesne oer a e aeara apaa 6 Installation Initial Setup amp Location Requirements 7 Loading the Syringe eeeeceeeeeeeeeeeeeeeeeeeeeeeee 7 Operation Getting Started Working WIth the PUMP ccsscsccccsecessennnseeaaanees 8 Check SVINGC eevatiisuisinsinin arial wnetnmataeaves 8 Maintenance mannen lian rette 8 Protecting Small Fragile Syringes 8 Hyper Terminal scenen 9 10 Appendices A Specificatl NS z i oers srki inr inatin 11 B Table of Popular Syringe Diameters 12 Gi Flow Rates rene herrer erkende 13 D Serial Communications Commands Queries and Responses 0000 14 15 Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component General Information Serial Number All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the underside of the mounti
9. ng plate Calibration All syringe pumps are designed and manufactured to meet their performance spec ifications at all rated voltages and frequencies Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Outside the U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authori zation number has been issued by our customer service department The customer is responsible for shipping charges Please allow
10. oltage CAUTION FAILURETO MAINTAIN POLARITY MAY RESULT IN DAMAGE TO THE UNIT AND WILL VOID THE WARRANTY 24 Vdc positive PIN 5 of 9 Pin D Sub 24 Vdc return PIN 1 of 9 Pin D Sub see input connections diagram Ground the Product This product is grounded through a ground stud located on the under side of the base plate To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the prod uct ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Any signal wire connec tions to the unit must be no longer than 3 meters Avoid Contact with Heatsinks Some components normally operate at temperatures exceeding 70 deg C To avoid injury do not touch any heatsinks Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component General Safety Summary Place Product in Proper Environment Enviromental Conditions Indoor use only Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to
11. on range Returns model and version number of firmware Returns target volume units in ml or pl depending on range value format nnnn nnn The returned value is an 8 character string with leading zeros converted to SP characters ACSII 32 decimal The fifth character is a decimal point ASCII 46 decimal Queries with string response RNG Returns range message character string either ML H ML M UL H UL M Error responses CR LF CR LF prompt Unrecognized command CR LF OOR CR LF prompt Entered value in out of range Publication 5415 002 REV B Declaration of Conformity Application of Council Directive s 73 23 EEC Standard s to which conformity is declared EN61010 1 1993 A2 1995 Manufacturer s Name Harvard Apparatus Inc Manufacturer s Address 84 October Hill Road Holliston Massachusetts 01746 U S A Type of Equipment Syringe Pump Model No 70 2202 High Force Pump Module I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date September 06 2002 C 1 8 Signature HARVARD BLE APPARATUS VP Engineering Operations Position
12. onent Appendix A ml Modular Pumping Component Specifications Accuracy 0 5 Reproducibility 0 1 Syringe Sizes 0 5mL min 50 60 mL B D Plastic max Flow Rate Min 0 0014mL hr w 0 5mL syringe Max 26 56mL min w 1mL syringe Linear Force 25lbs peak Drive Motor 0 9 Stepper Control L R Drive 0 75A per phase micro stepping Drive Ratio 2 4 1 Step Rate Min 6 8 sec step Max 416 7 msec step Pusher Travel Rate Min 2 9068 mm min Max 47 6 mm min Display None Keypad None Interface RS 232 Connectors RS 232 Power 9 pin D sub Power 15 to 40VDC 12W min use only a SELV approved Power Supply Dimensions 9 50 X 4 25 X 5 30 LX WX H 24 1cm X 10 8cm X 13 5cm Mounting Dimensions 9 00 X 3 75 Mounting holes for 4 8 screws 22 9cm X 9 5cm Weight 2 8 lbs 1 27kg Environmental Operating Temp 0 to 35 C natural convection cooling Storage Temp 20 to 70 C Humidity 20 to 80 RH non condensing Note Operating temperture range may be extended with forced air cooling Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component Appendix B Syringe Inside Diameter Terumo Stainless Steel Size Diameter Size Diameter 3 cc 8 95 mm 8 cc 9 525 mm 5 13 00 20 cc 19 130 10 15 80 50 cc 28 600 20 20 15 100 cc 34 900 30 23 10 60 29 10 Sherwood Monoject Plastic Size Diameter 1 cc 4 65 mm 3 8 94 6 12 70 12 15 90 20 20 4
13. prompt character indicates the status of the pump as follows prompt meaning ASCII code 3 When stopped ASCII 58 decimal gt When running forward ASCII 62 decimal When stalled ASCII 42 decimal Serial commands and their meanings Commands KEY Return to keyboard control Exit remote mode RUN Start infuse forward direction STP Stop motor CLV Clears volume accumulator to zero CLT Clears target volume to zero dispense disabled REV Reverses pumping direction Commands with numbers MMD number Set syringe diameter units are mm Rate is set to zero after MMD command ULM number Set flow rate and range units are microliters per minute MLM number Set flow rate and range units are milliliters per minute ULH number Set flow rate and range units are microliters per hour MLH number Set flow rate and range units are milliliters per hour 8 MLT number Set target infusion volume units are ml or pl depending on range Numbers can be between 0 and 1999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded Publication 5415 002 REV B 7 Harvard Apparatus mIl Modular Pumping Component Appendix D Serial Communication Queries DIA RAT VOL VER TAR Returns diameter value units in mm Returns rate value set in current range units Returns current accumulated infused volume units in ml or pl depending
14. rface Minimum of one inch 2cm clearance around the pump Appropriate environmental conditions Mount into an appropriately 953 rated enclosure or cabinet T 9 0 Recommended 8 63 22 86 Cabinet Cutout 21 9 Y Y 350 Dimensions 8 9 inches cm Mounting Holes Loading the Syringe for 8 Screw DN y e oN Release the syringe pusher 2 by pressing the bronze button 1 on the side of the pusher While holding the bronze button in slide the pusher to the left Raise the spring loaded syringe retainer 4 and swing it out of the way Lay the loaded syringe in the V shaped holder Swing the retainer so it holds the syringe in place Move the pusher so it makes contact with the syringe plunger Adjust pusher block bracket 3 amp Syringe retainer knobs 5 to capture plunger amp body of syringe HOT SURFACES Publication 5415 002 REV B Harvard Apparatus mIl Modular Pumping Component Operation Getting started Plug one end of the 6 ft Power RS232 extension cable supplied into JP1 connection on board Then plug the other end of the cable into your power supply and PC not included Refer to page 6 of this manual to reference the board connections Working with the pump The safest way to use the ml Modular Pumping Component is in the vol ume dispense mode The pump will automatically stop when target volume is dispensed
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