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Amersham™ Imager 600 Including

1. Tip Shift between lane profile image view and image tabs to verify the result of the band detection If the band detection is satisfactory then proceed to the final step in this table 2 If too many bands are detected then use the Sensitivity minus button to decrease the sensitivity If too few bands are detected then use the Sensitivity plus button to increase the sensitivity Amersham Imager 600 Operating Instructions 29 0645 17 AC 141 5 Operation 5 8 Analyze the image 5 8 3 Band detection Step Action 3 To make manual changes select one detection channel tab of Blue Green or Red above the image and select one or more relevant options e To create a new band point to the position in the image Result A new highlighted band appears e To shift a band position select the band and shift the band position up or down with the Position arrow buttons Position A Vv e Toadjust a band width select the band and adjust it with the Width plus and minus buttons Width e To delete a band select the band and press Delete e To delete all detected bands in the image select Clear 4 Repeat actions in step 3 for other detection channels if relevant 5 When you are done detecting bands press the Next button or select the MW button in the workflow The Previous button will bring you back to the previ ous step 142 Amersham Imager 600 Operating Instructions 29
2. Remaining tme Automatic exposure in progress 1 mn Exposing CCD status Blower postion 2013 November 11 10 33 Note Selecting Cancel will cancel the exposure and the Capture view will appear Amersham Imager 600 Operating Instructions 29 0645 17 AC 93 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Step Action 4 When the exposure is finished the image view appears displaying the im age s Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126 No colorimet ric marker was used The resulting image is displayed in gray scale i rams Linear Log Colorimetric marker was used The resulting images are displayed under Sample and Marker tabs e Select the relevant tab for the image you wish to display A selected tab is highlighted with blue text and frame Select both tabs simultaneously to see a composite color image of the Sample and Marker images 5 Select the Save button to save the images For other options after capturing see Options after capturing on page 102 94 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Chemiluminescence with semi automatic exposure Follow the instructions below to perform chemiluminescence capture using semi auto matic exposure time Ste
3. CCO status B Lower position 2013 November 11 10 52 9 Use the Select button to select which image or images you wish to save Then select the Save button Note If you select more than one image then the Library view will open after se lecting Save Amersham Imager 600 Operating Instructions 29 0645 17 AC 101 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Options after capturing Select one or more of the following actions after capturing e To save the image select Save This will allow you to reopen the image later to use the image tools to perform an analysis or to print the image See Section 5 7 Save and manage image files on page 121 for further instructions e To modify the display settings or view details of the image select the image tools to the right of the image See Image tools on page 118 for further instructions e To analyze the image select Analyze See Section 5 8 Analyze the image on page 133 for further instructions e To print the image select Print e To discard the image without saving select Done and confirm the following dialog 102 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 5 3 Colorimetric capture Introduction 5 Operation 5 5 Capture an image 5 5 3 Colorimetric capture Colorimetric capture is available in Amersham Imager 600 configurations as described in the following table Epi illumination Membrane Gel illu
4. The resulting images are displayed under Sample and Marker tabs e Select the relevant tab for the image you wish to display A selected tab is highlighted with blue text and frame Select both tabs simultaneously to see a composite color image of the Sample and Marker images ees 8 Select the Save button to save the images For other options after capturing see Options after capturing on page 102 Amersham Imager 600 Operating Instructions 29 0645 17 AC 99 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Chemiluminescence with incremental exposure Follow the instructions below to perform chemiluminescence capture with incremental exposure time Step Action 1 100 In the Capture tab select Chemiluminescence Result The options available for chemiluminescence capturing are displayed to the right on the screen Select Incremental exposure in the exposure drop down menu Capture Library te Chemiluminescence wi Chemiluminescence Repeat 12times J Stop when saturate Colorimetric Auto Semi auto Manual Incremental 4 Fluorescence Exposure Incremental Interval time 10s CCD status Lower position 2013 November 11 10 45 Set the number of repetitive exposures in the Repeat field using the plus and minus buttons Repeat 12 times Check the check box Stop when saturate if you wish the exposure to stop
5. Epi RGB Light ik Chemiluminescence Colorimetric Custom Trans UV 312 nm 4 Fluorescence Exposure Auto CCD status Lower position 2013 November 11 10 56 Result The options available for fluorescence capturing are displayed to the right on the screen Check the Epi RGB radio button and then check the relevant check box of Blue Green or Red Check the Colorimetric marker check box Select an exposure option in the Exposure drop down menu e Auto e Semi Auto e Manual press the Exposure time button and set an exposure time and then press Set For details see on page 55 If Auto or Manual exposure was chosen select Start Result A progress bar appears on the screen indicating the time remaining of the imaging process and the selected exposure option Proceed to the final step of this instruction If Semi Auto exposure was chosen select Next and wait for the pre exposure to finish Result A pre exposed image appears Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Step Action i When the pre exposure is finished a low resolution preview is displayed mAy p nt t H Q Q Ready Lower position 2013 November 12 12 17 Use the zoom tools and arrows to get an optimal view of the area of interest Choose Q to zoom in Choose Q to zoom out Note The colorimetric marker is
6. Comment imka names PONTO t445004_Ch Butts 2I 144502 Ch 20 4S Ch So soon Ija Amersham Imager 600 Operating Instructions 29 0645 17 AC 125 5 Operation 5 7 Save and manage image files 5 7 2 Save images File formats 126 Step Action e Select the location to save in the Where to save the file drop down menu Note Images can be saved on the instrument However when the instrument memory status is full the oldest file will automatically be deleted when a new image is saved It is therefore recommended to save the images on a USB flash drive or a network drive It is possible to expand storage capacity by connecting an external hard drive to the USB port at the rear of the instrument To personalize the image file name select the Optional image name prefix field to add a prefix to the auto generated image file name This opens a keypad view Type your text and then select Done to return to the previous view To add a comment select the Comment field This opens a keypad view Type you comment and then select Done to return to the previous view Select Save to save the file s The saved files can be accessed via the Library tab See Section 3 5 2 Description of the Library tab on page 44 for more details If capturing is finished for the day switch off the instrument by pressing the On Off button on the front panel Wait until the instrument is shut down LED lights are turned off Then p
7. 600 UV 600 QC 600 RGB Chemiluminescence with colorimetric marker Membrane Black Tray White Insert 600 600 UV 600 QC 600 RGB Colorimetric Epi illumination e Membrane e Gel Black Tray White Insert or White Trans Tray 600 600 UV 600 QC 600 RGB Colorimetric Trans illumination Fluorescence RGB Epi illumination e Gel e Membrane White Trans Tray Diffuser Board Black Tray 600 QC 600 RGB 600 RGB Fluorescence Trans UV Gel UV Trans Tray transparent Gel sheet 600 UC 600 QC 600 RGB Note With regards to third party patents related to sample preparation etcetera we recommend that you consult a lawyer or patent attorney on obtaining appropriate licenses 86 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 4 Place the tray in the instrument 5 4 Place the tray in the instrument Place the tray This section describes how to place the sample on the tray and how to place the tray in the correct tray position in Amersham Imager 600 Step Action 1 Select a tray and accessories suitable for the capturing method 2 For Chemiluminescence with colorimetric marker and Colorimetric Epi capturing place the White Insert inside the Black Tray 3 Place the sample s on the tray or on the White Insert Take care not to cover the areas outside any visible markings on the tray or the White Insert as they ma
8. Frequency 50 60 Hz Supply Voltage 100 240 V c US Max Power 250 W LISTED Protection Class IP21 nee Manufacturing Year ACEO Made in Japan 405N120051 Symbols used on the label The following symbols are found on the label on the instrument Meaning Warning Read the Operating Instruction before using the system Do not open any covers or replace parts unless specifically stated in the Operating Instruction The instrument complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand Amersham Imager 600 Operating Instructions 29 0645 17 AC 21 2 Safety instructions 2 2 Labels Meaning The instrument complies with applicable European directives This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized repre sentative of the manufacturer for information concerning the de commissioning of equipment This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics This symbol indicates that the instrument has been certified by a AeA Nationally Recognized Testing Laboratory NTRL An NRTL is an aBoRaTORY EQUIPMENT Organization that the Occupational Safety and Health Adminis
9. Unevenness appears on the image The LED is deteriorated Turn off the instrument and contact your GE representa from age tive Alamp inthe UV transil Turn off the instrument and contact your GE representa luminator is broken tive Noise appears in an image exposed using the UV Trans tray If Then The UV Trans Tray is de The UV Trans Tray is contaminated Clean the tray teriorated from age Artefacts appear ina Colorimetric Epi image If Then The correct tray is used Rotate the gel sample 90 positioning the gel so that the lanes of the gel are parallel to the long side of the tray Amersham Imager 600 Operating Instructions 29 0645 17 AC 159 7 Troubleshooting 7 3 Problems with the software 7 3 Problems with the software This section describes possible causes and corrective actions for problems with the software If the problem persists after taking the suggested corrective action contact your GE representative After exposure no image is displayed on the screen The light source does not function prop Contact your GE representative erly The sample is not in position Check the sample position The screen of Amersham Imager 600 is not displayed on the connected tablet computer Wireless access points are not properly Contact the network administrator or set connect a monitor mouse and keyboard directly with a cable The buttons do not appear onan external
10. A progress bar appears on the screen indicating the time remaining of the imaging process and the exposure time Proceed to the final step of this instruction F If Semi Auto exposure was selected select Next Result A low resolution preview appears 6 Point to the area of interest on the image There are two options i Use the button to point to a specific point to watch or Use the button to point out two diagonal corners of an area Then press Start exposure 7 When the exposure Is finished the image view appears displaying the image Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126 E oe Ca j i Split image 4 LI td Erprsue teria ts 8 Select the Save button to save the images For other options after capturing see Options after capturing on page 102 116 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 6 View the image 5 6 View the image Image view screen When the capture is performed a view with the captured image s is displayed on the screen The content on the screen varies with the capturing method and chosen exposure option The following illustration and table describe the image result view of a Fluorescence Epi RGB multichannel capture For a detailed description of the image result view see Sec tion 3 5 3 Description of the image view on page 46 2 1 E Display
11. be used in the subsequent analysis Move comer point gt 2 Open the background subtraction step with the button Background Background Background is used to select method for background subtraction 50 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 4 Description of the analysis workflow Step _Illustrationofstep Description 3 Open the band detection step with the button Band Detection ty Auto detect Bands Sensitivity Band Detection is used to detect the bands in each defined lane Edit individual band Position A v Width Delete Clear 4 Open the MW calibration step with the button MW Calibration 100bp DNA Edit Add new J 1500 EN MW Calibration is used to create a molecular weight z 1400 calibration curve based on a selected standard marker J 1300 t nnn Curve type linear log cubic spline View plot 5 Open the normalization step with the button Normalization Normakzation Please select control band s in each lane ede ee ee Normalization is used to select bands to be used for normalization Amersham Imager 600 Operating Instructions 29 0645 17 AC 51 3 Instrument description 3 5 Instrument software description 3 5 4 Description of the analysis workflow Step Illustration of step Description Open the summary with the button Summary Summary is used to present a
12. 3 Ifthe error persists refer to this troubleshooting guide for possible solutions 4 Ifthe error cannot be resolved with this troubleshooting guide contact your GE representative Amersham Imager 600 Operating Instructions 29 0645 17 AC 155 7 Troubleshooting 7 1 General problems 7 1 General problems This section describes possible causes and corrective actions for various problems with the instrument If the error persists after taking the suggested corrective actions contact your GE representative Atypical noise is heard Noise comes from the camera head then a camera head failure has occurred Then Turn off the power switch immediately and contact your GE representative Atypical odor is emitted Atypical odors are emitted from the camera head then a camera head failure has occurred Turn off the power switch immediately and contact your GE representative Atypical odors are emitted then an instru ment failure has occurred Smoke is emitted Smoke is emitted from the camera head then a camera head failure has occurred Turn off the power switch immediately and contact your GE representative Turn off the power switch immediately and contact your GE representative Smoke is emitted from the instrument then an instrument failure has occurred Turn off the power switch immediately and contact your GE representative The LED blinks The ERROR LED blinks a hardware error occurre
13. 5 Operation 5 8 Analyze the image 5 8 4 Molecular weight assignment Step Action 5 Select View plot to see the plot 6 Select Close to return to the previous view 7 When you are done select the Next button or select the Normalization button in the workflow The Previous button will bring you back to the previ ous step Note The calculated molecular weight is presented in the summarized table in Summary Add a molecular weight standard marker Step Action 1 Select the Add new button Result The following view appears Nome new imio Uag mane 0 Values 2 Select the Name field and type the name of the marker 3 Select the unit from the Unit drop down menu 4 Type a value and select the Set button Result The value will appear in the bottom of the list to the left on the screen 5 Select the Save button to save the added values and to return to the MW Calibration view Select Cancel to leave without saving changes 144 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 4 Molecular weight assignment Edit a marker Step Action 1 Select the Edit button Result The following view appears Hame 100bp DHA Liest mrt a hee 1500 ra 1400 d ij 1200 Hgg ipt o0 Bgo THD ao i He O O O Oo em WF AD k a ES 2 Select the value from the list The marked values are highlighted 3 Type a new value and select the
14. Bock j amp E Qa TE mA u b LE Minimum profile Use this option to subtract all the background below the minimum value in the lane profile view Roing dat X 1 Qa at A x CORON i 138 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 2 Background Subtraction Description Rolling ball Use this option to subtract the background below the graph as If erasing it using the radius of a ball A small radius will subtract more background than a large BBB orcs a Subtract background Step Action 1 If an image of the sample is displayed select the lane profile button WM Result A screen will appear displaying the lane profile graphically with the current lane number displayed in the drop down list and the lane profile button in blue 2 Choose one of the relevant method radio buttons e None e Rubber band e Minimum profile e Rolling ball and then select the Radius plus or minus buttons to set the radius for the virtual rolling ball eraser to set the new baseline of the graph Radius 30 f Tip The smaller the radius the more background will be removed Amersham Imager 600 Operating Instructions 29 0645 17 AC 139 5 Operation 5 8 Analyze the image 5 8 2 Background Subtraction Step Action 3 Select Subtract to subtract the background Result The lane profile view Is displayed with the background subtracted B
15. Imager 600 air intakes even if the other environmental require ments have been met Do not install the equipment near a window Avoid direct sunlight Ensure blinds are attached to nearby windows Do not place objects near the power outlet to ensure easy access to the power cord for disconnection in case of emergency Operating temperature humidity conditions Transportation storage conditions Temperature 18 C to 28 C with temperature fluctuation below 10 C per hour or lower Humidity 20 to 70 RH no dew condensation Note When the above conditions cannot be satisfied take appropriate actions Temperature 25 C to 70 C Humidity 5 to 95 RH no dew condensation Note When the above conditions cannot be satisfied take appropriate actions Amersham Imager 600 Operating Instructions 29 0645 17 AC 165 8 Specifications Parameter Condition Installation location conditions 4 Do not install the instrument where the temperature can vary widely with temperature fluctuation above 10 C per hour Do not install the instrument near a heat source such as a radi ator Do not install the instrument where It may get wet or flooded Do not install the instrument where it may be exposed to corro Sive gas Do not install the instrument in a dusty environment Do not install the instrument in a place constantly or excessively exposed to vibration or impacts 7 Donotinstall the instrument in
16. Owete 4 Use the search function to limit the list e Search by date Use the date format YYYYDDMM Example 20133011 e Search by keyword Select Search to perform the search Delete the information in the search fields and select Search to reset the list 5 Select the files to be copied or deleted by checking the file s check box Note A maximum number of 5 files can be copied at one time You may repeat this step to copy additional files 6 e Select the Copy button to copy the selected files e Select the Delete button to delete the selected files Note The copied files will be downloaded as a zip file with an automatically gener ated name The download location and procedure will vary with the chosen web browser s download settings 80 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 Operation About this chapter This chapter describes how to operate Amersham Imager 600 Prior to operating the Amersham Imager 600 system it is important that you have read and understood the safety information Make sure to read the relevant safety instructions prior to operation See Section 2 1 Safety precautions on page 13 for details WARNING Do not operate Amersham Imager 600 in any other way than de scribed in the User Manual In this chapter Section See page 5 1 Operation flow chart 82 5 2 Turn on the instrument 83 5 3 Select tray 86 5 4 Place the tray in the instrum
17. Rotate 90 Split image Exposure times Blue 0 9s Green 1 0s Red 1 45 Print Analyze Save Function The captured image is displayed Tabs for captured images Use the tab to select or deselect images of the different detection channels Note A displayed channel is indicated with a tab in blue text and blue frame All three channels can be selected simultaneously A non displayed channel is indicated with a black text and gray frame Image tools menu Use the buttons to choose tools to modify the image display and to examine the image The selected image tool options Amersham Imager 600 Operating Instructions 29 0645 17 AC 117 5 Operation 5 6 View the image Image tools A captured image can be visually modified and examined with the image tools found to the right on the screen The image tools can be used on images immediately after capture and on a previously saved image Use one or several of the tools to modify or to examine the image Note The original image data remains unaltered The following table describes each tool in the image tools menu in detail Description Illustration The Display tool is displayed by default after capturing or opening an image Select the Invert check box to invert the display of the im Disploy age that is to change from white background and black Invert bands to black background and white bands Rotate Use the Rotate 90 degrees left or ri
18. Set button Result The new value will appear in the list Note You may need to scroll to see the new value 4 Select the Save button to save the edited values and to return to the MW Calibration view Select Cancel to leave without saving changes Delete will delete the entire marker group Note To delete a value select the X next to the value Amersham Imager 600 Operating Instructions 29 0645 17 AC 145 5 Operation 5 8 Analyze the image 5 8 5 Normalization 5 8 5 Normalization Description This is the fifth step in the analysis workflow The selected image is displayed on screen and the button Normalization is highlighted in the workflow Normalization calculates band volumes relative to one or more reference bands Select reference band s in the lane and all other bands in that lane will be normalized against the selected band s If multiple reference bands are selected the reference value is set as the average of the selected band volumes Normalization is useful when comparing relative band volumes in different lanes The options for normalization are presented next to the image Normalize bands 146 Follow the instructions below to perform normalization Step Action 1 Select one or two control bands in a lane Result The selected band s appear highlighted Note Control bands can only be selected in single channel view cae ETAT o 7 A Please select comrol bandis in each lane 123
19. a place exposed to direct sunlight Operation site Indoor use Maximum operating altitude 2000 m or lower Noise 70 dB A or lower Fast Lmax 60dBi A and average Leq lt 54dB A 1 m from the instru ment Protection provided by enclosure IP21 Overvoltage category Transient overvoltage category Il Rated pollution applied Pollution degree 2 166 Amersham Imager 600 Operating Instructions 29 0645 17 AC Index A Amersham Imager 600 Feature overview 32 Hardware components 35 Analyze 49 C CE Conformity 8 CE marking 10 Connect to a network 73 DHCP network 74 Static IP network 75 Contrast tool 118 D Display tool 118 E Emergency procedure 25 F File formats 126 H Hardware components 35 Installation 60 Site requirements 61 164 Transport 63 Intended use 6 Intensity tool 118 K Keypad 54 L Labels 21 LED Indicators 38 M Maintenance 70 150 Amersham Imager 600 151 Amersham Imager 600 Operating Instructions 29 0645 17 AC Index Sample trays and filter 153 Manufacturing information 8 Monitor requirements 19 Moving instrument 63 Multichannel imaging 108 N Notes and tips 7 O Operation Place the sample 87 Sample and tray combina tions 86 Save the image 121 P Place the sample 87 Print Printer 46 Purpose of this document 5 R Recycling information 26 Regulatory compliance 10 S Safety noti
20. and tips Note Anote is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text for example Power Amersham Imager 600 Operating Instructions 29 0645 17 AC 7 1 Introduction 1 2 Regulatory information 1 2 Regulatory information This section describes the directives and standards fulfilled by Amersham Imager 600 Manufacturing information The table below summarizes the required manufacturing information For further infor mation see the EC Declaration of Conformity DoC document Requirement Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden CE conformity This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the EC Declaration of Conformity is available on request Directive 2006 42 EC Machinery Directive MD 2004 108 EC Electromagnetic Compatibility EMC Directive International Standards This product fulfills the requirements of the following standards Standard Description IEC 61
21. are displayed The illustration below de scribes the main components of the image view 3 2 co Sample Marker Q c x Display Invert Rotate 90 90 Split image Function Display of the captured image Note Incremental exposure will result in several images displayed two at a time as they are exposed Navigate among the images using forward and back ward arrows to the right of the image CCO status Lower positon 2013 November 11 10 52 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 3 Description of the image view Function 2 Tabs for captured images A blue text and frame highlights the displayed imagels Note By selecting two or more images a composite image is displayed For more information on displayed images for the different capturing methods see Section 5 5 Capture an image on page 89 If the colorimetric marker option was checked the tabs will display Sample and Marker If a multichannel fluores cence capture was per formed the tabs will dis play the selected channels of Blue Green and Red e 3 Information icon Use to access additional image and exposure information such as image file name and exposure time Amersham Imager 600 Operating Instructions 29 0645 17 AC 47 3 Instrument descrip
22. automatic sample is of interest A short pre exposure is performed which results in an image This allows you to select the area of the sample image to focus on The software automatically calculates the exposure time for the specified area Note Semi automatic exposure is not available for multichannel fluorescence capture The intensity of the im Select Manual exposure and set the exposure time man age is inadequate after ually automatic exposure or a known exposure time is required Fluorescence Epi RGB This method is used for fluorescence imaging single or multichannel Follow the instructions below to perform Fluorescence Epi RGB capture 108 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Step Action i In the Capture tab select Fluorescence Result The options available for fluorescence capturing are displayed to the right on the screen Capture Library e Fluorescence Epi RGB Light walk Chemiluminescence Ka Colorimetric Custom Trans UV 312 nm 4 Fluorescence Esir rE e CCD status amp amp Lower position 2013 November 11 10 56 2 Check the Epi RGB radio button and then check the relevant detection channel check boxes of Blue Green and Red Note More than one check box can be checked for simultaneous multichannel capture Lape Library ia Ep Rue vik Chem uminescence Blue wf Grees
23. automatically by the DHCP server Workgroup WORKGROUP Contact your system administrator for proper settings B Ready amp Lower position 2013 September 9 10 59 3 If desired check the Workgroup field and use the keypad to enter the workgroup Note For detailed information on how to use the keypad see Keypad view on page 54 4 Select OK Result An automatic connection to the DHCP network is established within a couple of seconds Tip Reserve the IP address to Amersham Imager 600 for easier access of its net work resources Connect to a Static IP network Follow the instructions below to connect to a Static IP network Step Action 1 Connect the network cable to the Ethernet port on Amersham Imager 600 Amersham Imager 600 Operating Instructions 29 0645 17 AC 75 4 Installation 4 4 Network 4 4 1 Network setup Step Action 2 Check the Static IP radio button in the Network window Capture Library i Network DHCP IP address Static IF Subnet mask Static IP Enter network Gateway settings manually Contact your system Workgroup administrator for proper settings CCD status H Lower position 2013 November 11 10 29 3 Select the 1 IP address field and use the keypad to enter the IP address 2 Subnet mask field and use the keypad to enter the subnet mask If desired select the e Gateway field and use the keypad to enter the router e Workgroup field and use the keypad to enter the wo
24. configurations are described in the following table 600 600UV 600QC 600 RGB Epi RGB Custom Trans UV Note The Custom method allows for different combinations of the blue green or red light sources and an emission filter of choice Fluorescence capture options The table below illustrates sample types for fluorescence capturing Sample type Tray and accessory Light source location in placement only in the side lower tray position Gel e g Black Tray Epi Blue Green and Red Cy 2 Cy 3 Cy 5 fluorescence top Membrane e g ECL Plex Black Tray Epi Blue Green and Red Cy 2 ECL Plex Cy 3 fluorescence top ECL Plex Cy 5 Gel e g EtBr SYBR Green UV Trans Tray transpar Trans UV bottom ent gel sheet Amersham Imager 600 Operating Instructions 29 0645 17 AC 107 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Fluorescence exposure options There are three exposure options for fluorescence capturing In the following table the benefits of each exposure option are described Optimal dynamic range Select Auto for automatic exposure is required A short pre exposure is performed to determine the signal intensity The system will use this information to calculate which exposure time will give the highest possible signal below saturation to enable accurate quantification of the sample A specific area of a Select Semi
25. from the device Step Action 1 Select the file s to delete 2 Select the Delete button Result The following window will appear Delete x Are you sure to delete selected image files 3 Select Yes button to delete the file s Copy or move files Follow the instruction to copy and move files Step Action i Select the file s to copy or move 130 Amersham Imager 600 Operating Instructions 29 0645 17 AC Step 5 Operation 5 7 Save and manage image files 5 7 3 File manage options Action Select the Copy Move button The following window will appear Capture brary a Copy Move Move or copy selected files to USB 2 Selected files 4 2 0 1 s pt06 20131010_153426_Ch A 4 2 0 1 Pt06 UR 70131010 232708 Ch 4 2 0 2 s Pt06 UR 20131010 237117 Ch Move CCD status Lower position 2013 November 11 10 43 Select the location to copy or move the file s to in the drop down menu in the Move or copy selected files to field Select the Copy button to copy the file s to the desired location Select the Move button to move the file s to the desired location Note When a file is copied the file will remain in the original location thus be stored in two places Rename files Follow the instruction to rename files Step Action 1 2 Select the file to rename Select the Rename button Result The keypad will appear Use the keypad to change the name of t
26. is ready for use e Orange The CCD temperature is not within specifications Wait for a green light before proceeding Method sensitive displays The display on the screen shows the selected image capturing method and presents the options relevant for that method Depending on system configuration you may or may not have access to all methods The following section describes all available method displays in the Amersham Imager 600 product family For details on system configurations see Section 3 4 Instrument hardware description on page 54 Chemiluminescence The illustration below shows the main components of the Capture tab with the default setting Chemiluminescence image capturing method and automatic exposure time Auto Capture Library tei kis Chemiluminescence oy 2 Colorimetric marker 1 ee E o Colorimetric 4 Fluorescence CCD status Mj Lower position 2013 November 11 10 30 Part Function i Chemiluminescence button The selected chemiluminescence image capturing method Amersham Imager 600 Operating Instructions 29 0645 17 AC 41 3 Instrument description 3 5 Instrument software description 3 5 1 Description of the Capture tab Function Colorimetric marker check box Use the check box for samples with col orimetric marker Exposure drop down menu Use to select the exposure option for chemilu minescence image capturing Auto Semi Auto Manual and Increme
27. lower tray position 2 If relevant point to an area of interest to zoom in on 3 Adjust the brightness of the image to a satisfactory level by using the plus and minus buttons next to the Brightness value 4 Adjust the focus to a satisfactory level by using the arrows above and below the Focus value 5 Select OK to save the settings Cancel will close the view without saving changes 6 The new focus will be available for captures until a new capturing method is selected or until the instrument enters standby mode Prior to the capture confirm the use of the new focus with Yes Selecting the No button cancels the adjustments to focus and the normal fixed focus setting default is once again in use 68 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 3 System settings 4 3 3 Instrument indicator 4 3 3 Instrument indicator The Instrument indicator is a useful tool when several Amersham Imager 600 instruments are used in the same location It identifies which instrument is connected to a certain tablet computer Select the Instrument indicator button This activates the Run LED light on the instrument it is connected to Power Run Error go A oO Amersham Imager 600 Operating Instructions 29 0645 17 AC 69 4 Installation 4 3 System settings 4 3 4 Service 4 3 4 Service Introduction In the Service view the following information can be found Capture Library pe Service
28. make sure to restart the system at least once a day to remove temporary files that may take up unnecessary memory space Amersham Imager 600 Operating Instructions 29 0645 17 AC 149 6 Maintenance 6 Maintenance About this chapter To ensure optimal performance Amersham Imager 600 and accessories should be maintained regularly This chapter describes the maintenance that should be performed on a regular basis For all safety cautions and notices relevant to maintenance please see Maintenance on page 19 WARNING Electrical shock hazard All instrument repairs or modifications should be performed by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation WARNING Do not use excessive amounts of liquids for cleaning the Amersham Imager 600 instrument This may result in instrument malfunction or electric shock NOTICE When performing maintenance of the equipment remove the tablet computer There is risk of injury if it falls on your foot In this chapter Section See page 6 1 Instrument 151 6 2 Accessories 152 6 3 Regular inspections 154 150 Amersham Imager 600 Operating Instructions 29 0645 17 AC 6 Maintenance 6 1 Instrument 6 1 Instrument Use a soft dry cloth to clean the exterior of Amersham Imager 600 For marks that are hard to remove wipe with a soft cloth slightly dampened with water and a neutr
29. monitor Then If The monitor resolution is too low Use monitors with SVGA resolution or higher 160 Amersham Imager 600 Operating Instructions 29 0645 17 AC 7 Troubleshooting 7 5 Problems with the software The software is non responding Transferring large amounts of files to a Avoid transferring during operation connected computer during operation can impair the system s performance Problems with tablet computer connection The tablet computer screen turns white when starting the software Make sure you are connected to the cor rect network on the tablet computer that is same network as Amersham Imager 600 If the instrument has recently been switched on it may take a few minutes for the instrument to come online The wireless network can be detected before the instrumented is fully connected Normally the instrument becomes online within 5 minutes Restart the software on the tablet com puter The software display on the tablet com puter screen has frozen after a long time of inactivity Press the home button on the tablet computer and restart the software No or few button presses are registered Use gentle and distinct touches on the tablet computer to control buttons in the software A light and short tap is best registered by the software Amersham Imager 600 Operating Instructions 29 0645 17 AC 161 7 Troubleshooting 7 3 Problems with the software T
30. move images from the instrument to ensure available storage space If the instrument flash drive runs full the oldest images will automatically be removed when a new image is captured Tip An external hard drive can be added to the USB port at the rear of the instru ment to expand available storage capacity e Onan external USB flash drive e Ona network drive Note Network access is possible providing the instrument has been granted access to the network location For more details see Section 4 4 Network on page 72 Connect a USB flash drive A USB flash driver is automatically installed when the USB flash drive is connected to Amersham Imager 600 Note Password protected USB flash drives are not supported Add new save location Follow the instructions to add a location for file saving Note To enable access to a location for saving files over the network the specific folder must be shared and read write privileges must be added for the Amersham Imager 600 instrument See Section 4 4 Network on page 72 Step Action 1 Select the settings icon in the top right hand corner of the screen it 122 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 7 Save and manage image files 5 7 1 Save locations Step Action 2 Select the Save locations button Result The following screen appears Capture Library a ASS temine Of edi koriin fo sine images afer capture Ge copying in Gbrary i
31. network connections and set system settings Note Installation or transport of the Amersham Imager 600 instrument must be per formed by GE authorized personnel The instrument contains parts that may be damaged unless they are secured for transportation and handled correctly In this chapter Section See page 4 1 Site requirements 61 4 2 Instrument setup 63 4 3 System settings 64 4 4 Network 72 60 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 1 Site requirements 4 1 Site requirements Environmental conditions The following table describes the environmental requirements for Amersham Imager 600 CAUTION Do not use the instrument in a room with a temperature above 28 C Higher temperatures does not allow the CCD to cool down properly to 25 C Parameter Condition Free space required around Front of instrument the operation panel side 40 cm Amersham Imager 600 Right 10 cm Left 10 cm Rear 10 cm Top 10 cm Placement A stable laboratory bench with a load capacity of 980 N 100 kg or higher The bench must be level within 2 two degrees Other conditions 1 Take into consideration the work flow and ancillary facilities when planning the installation 2 Any required construction and electricity air conditioning work must be completed prior to installation 3 Itis not desirable to have a heat source near the Amersham Imager 600 air inta
32. not displayed in the preview 8 Choose one of the two options displayed to the right of the image to point to the area of interest Choose Ed then point to one point of interest in the image Result The selected point will be highlighted with an X Choose and then point to two diagonal corners of an area of interest in the image Result The selected area will be highlighted with a square Use the right hand side arrow buttons to move the marked point or area 9 Select Start exposure and wait for the exposure to finish Amersham Imager 600 Operating Instructions 29 0645 17 AC 113 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Step Action 10 When the exposure is finished the image view appears displaying the images Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126 e Select the relevant tab of Sample and Marker for the image you wish to display A selected tab is highlighted with blue text and frame e Select both tabs simultaneously to see a composite color image of the Sample and Marker images starve i H o FS f m _ gi ET 11 Select the Save button to save the images For other options after capturing see Options after capturing on page 102 Fluorescence Custom The Custom option is designed to allow for various customized combinations of wave lengths of the illuminating light and f
33. or when opening an already captured image The workflow is performed in five steps and results in a summary After completing a step navigate to the next step by selecting the Next button For more details see Sec tion 5 8 Analyze the image on page 133 Description The following illustration shows the start screen and first step of the analysis 2 3 Sample Marker WA Lane Creation Number of lanes 42345678 n Al S Lane width Function Contrast button Use the button to change the contrast of the image Image view button Use the button to activate the image view Lane profile button Use the button to display separate graphical lane profiles Zoom out button Use the button to zoom out Zoom in button Use the button to zoom in Analyze workflow buttons Use the buttons to navigate in the analysis workflow Amersham Imager 600 Operating Instructions 29 0645 17 AC 49 3 Instrument description 3 5 Instrument software description 3 5 4 Description of the analysis workflow Analysis options The illustration below describes the workflow steps and options Step Illustration of step Description Background method None Rubber band Minimum profile Rolling ball Radius 20 Subtract 1 Open the lane creation step with the button Lane Creation Number of lanes M Lanes Lane width 76 Lane Creation is used to define and position lanes to
34. power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect Amersham Imager 600 Operating Instructions 29 0645 17 AC 25 2 Safety instructions 2 4 Recycling information 2 4 Recycling information Introduction This section contains information about the decommissioning of Amersham Imager 600 Decontamination The equipment and the accessories must be clean from contaminants before decom missioning and all local regulations must be followed with regard to waste disposal Samples are to be disposed of according to local regulations Disposal general instructions When taking the Amersham Imager 600 out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substance The lamps in Amersham Imager 600 configurations 600 QC 600 UV and 600 RGB contain mercury vapour These must be recyled or disposed of in a manner compliant with national and local environmental regulations Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of the equipment 26 Amers
35. tablet computer 157 7 Troubleshooting 7 2 Problems with image quality 7 2 Problems with image quality An appropriate image does not appear An image is overex posed Shorten the exposure time An image is underex posed Prolong the exposure time The focus is not correct ly adjusted Adjust the focus temporarily See Section 4 3 2 Focus on page 67 If the problem persists contact your GE representative The tray white insert or window covering the light source are dirty Clean the tray white insert or the window covering the light source The wrong sample tray is used Change to the correct sample tray The wrong light source is selected Select the correct light source The size of the object exposed does not coin cide with the exposed area Light leaks on the image The door to the instru ment is not completely closed Place the sample correctly on the sample tray and place the tray in the correct tray position Close the door to the instrument and expose the image again the instrument is ex posed to direct sunlight Avoid direct sunlight Amersham Imager 600 Operating Instructions 29 0645 17 AC 7 Troubleshooting 7 2 Problems with image quality The image appears misty Dew is condensing in Turn off the instrument with the On Off button on the the optical system front panel and wait until the ambient environments meets the specifications
36. wf Red i Colorimetric E COO status M Lower position 2013 Hovener 11 11 13 3 Choose an exposure option in the Exposure drop down menu e Auto and proceed to step 6 or e Manual and proceed to the next step Amersham Imager 600 Operating Instructions 29 0645 17 AC 109 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Step Action 4 110 If exposure option Manual was selected then select the Exposure time Set button Exposure time Set Note If more than one detection channel was selected the Exposure time button displays Set If only one detection channel was selected the button displays the latest set exposure time Result This opens the Set Exposure time view Set the exposure time for each detection channel by selecting the color tab enter the value Liteary ei You can use beth the rumene keys and tha butions bo sal fie CC status amp Lower position 2013 November 11 1231 When all values are entered press Set Result This will close the view For details see on page 55 Select Start A progress bar appears on the screen indicating the time re maining of the imaging process and the selected exposure option Amersham Imager 600 Operating Instructions 29 0645 17 AC Step Action 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture 7 When the exposure is finished the image view appears displaying the images Amersham Imager 600 crea
37. 010 1 EN 61010 1 Safety requirements for electrical EN 61010 1 lat IEC 61010 2 010 IEC equipment for measurement con est edition 61010 2 081 UL 61010 1 trol and laboratory use aligned with EN CAN CSA C22 2 No 61010 2 081 61010 1 EN 61326 1 IEC 61326 1 Electrical equipment for measure EN 61326 1 EN FCC Part 15 B Class A ment control and laboratory use 301 489 1 ICES 003 Class A EN 301 EMC requirements aligned with 489 1 EN 301 489 17 2004 108 EC 8 Amersham Imager 600 Operating Instructions 29 0645 17 AC 1 Introduction 1 2 Regulatory information Standard Description EN ISO 12100 Safety of machinery General princi ples for design Risk assessment and risk reduction EN 60529 Degrees of protection provided by enclosures UL94 V2 Standard for safety of flammability of plastic materials for parts in de vices and appliances testing FCC standard re This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the Federal Communications Commission FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not in stalled and used in accordance with the instruction manual may cause harmful interfer ence to radio communications Operation o
38. 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 4 Molecular weight assignment 5 8 4 Molecular weight assignment Description This is the fourth step in the analysis workflow The selected image is displayed on the screen and the button MW is highlighted in the workflow The options for molecular weight assignment are presented beside the image Molecular weight is calculated using standard molecular weight MW markers Assign molecular weight The following illustration and table below describe how to assign molecular weight Note Molecular weight calibration can only be performed in single channel view Step Action 1 Select the lane containing the molecular weight marker Result The selected lane appears highlighted Color Eli MW Calibration Rainbow marker g aQ mM A AA t 2 Select the standard molecular weight marker from the drop down menu If the correct marker is missing e Addamissing marker by selecting Add new button See instructions below e Edita marker by selecting the Edit button See instruction below 3 Deselect any missing bands in the sample marker lane by unchecking rele vant check boxes 4 Select the fitting function by marking one of the Curve type radio buttons e Lin log which presents a linear fit to logarithmic data e Cubic spline which presents a smooth polynomial that is piecewise defined Amersham Imager 600 Operating Instructions 29 0645 17 AC 143
39. 13 21 25 26 2 7 Table of Contents 535 Color mere Cape sirana Ensa eemerT Tem rete tre Terre ee AAR 5 5 4 Fluorescence CODEC ssaccsascapsvsacuracunssessitiietkashcssoia taeepan teow btenctieaeeaetsieusivieainetdaaamanutomnenie MIS NMS ccs scetr cee sect E E E E E N TAE VCO mandge WMTW SS serenana E STEE Sr SAVE VOCG NOS ssena A ERTA NEE 5 7 2 SOVE OOS a tiearaeeirt dienes E aan esta ei ocean 575 Filemaondge OPUNE greaenneer ane tem aun te ntnT Br kn AR 56 Anayze tne 9 6 01 6 sreda arn ne on on TTT et OCOD Stee ee 5 8 1 Lane cred syror eres rrr TE TA AE EE ee E eee eee Te 582 Backoround Suo roco seai EE een oe See 5 05 Band Gele annan NERE ter ever TSA ER 5 8 4 Molecular weight ASSIQNMENE seccssscsssssessssesssessssessssecsssesssecsssssssessssecssuessuecssecsssecessecsseeeseeesses 5 8 5 Ne g 60 01 a O a e E E E E Tents te tye 5 8 6 SUMMATFY nonsssssssnnsssssssnessssssssssnssssssssssssrissssesntsnsssttenssssntttnssnstenensstensssssttttnsssstotnssntotnssssnnotnnsnenetesseneent S9 Tumore SI raies o C P E a ante gocsseseetesaneeecieenes 6 1 IS TMD E a E E E E AET E E AAE E E E EE E A A Ce A O a E ee eee ee ee 6 3 Regular MS 91 21616 0 9 ca een noe Poo nnnE et ne PS nt Er Da enone vO en TIS iN UNC aa aeras vegeta teneceeseiees exer ateetaninteseinseidatmsarsieuieee ra E GL Genara poen aera vO E worn ne aE Le FOI winimage gudhi y eessen occa teanctsaechsesteeapacaiecoeeeeaceeaste 7 3 Propilems with tne RO aaier AA aR eesoaie
40. 456 H Oo e i eb a la 4 Q QA am AAt tS Ee 2 When you are done select the Next button or select the Summary button in the workflow The Previous button will bring you back to the previous step Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 6 Summary 5 8 6 Summary Description This is the final view of the analysis workflow The analyzed image is displayed on the screen and the Summary button is highlighted in the workflow The illustration and table below describe the summary view Function The analyzed image Tabs for displaying details Print button Use to print the summary on a connected printer Columns displaying Ch the detection channel Lane the number of the lane change the sort order by selecting up or down arrow in the column heading Band the number of the band from top to bottom Volume the integrated signal intensity in the specific band Lane the relative band volume within a lane in percentage Normalized value the band volume relative to the control band volume Mw the calculated Mw Rf the relative mobility of the band Amersham Imager 600 Operating Instructions 29 0645 17 AC 147 5 Operation 5 8 Analyze the image 5 8 6 Summary Finalize and save analysis 148 Step Action 1 Review the summary 2 If the analysis is completed press Done Result The image view appears 3 To
41. Always keep the Operating Instructions at hand when operating Amersham Imager 600 Do not operate Amersham Imager 600 in any other way than described in the user documentation If you do you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use Amersham Imager 600 is a CCD camera system that produces digitial images of samples in gels or membranes in three different modes chemiluminescence colorimetric and fluorescence Amersham Imager 600 is intended for research use only and shall not be used in clinical procedures or for diagnostic purposes Safety notices This user documentation contains safety notices WARNING CAUTION and NOTICE concerning the safe use of the product See definitions below WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood 6 Amersham Imager 600 Operating Instructions 29 0645 17 AC 1 Introduction 1 1 Important user information CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes
42. Amersham Imager 600 Including 600 600 UV 600 OC 600 RGB Operating Instructions Original instructions Page intentionally left blank Table of Contents Table of Contents L MoCo eea IL WMO COMMIS SIO UE ONY ssrin n EE O eae 1 2 Regulatory TM TOUIG VOI zest deen cass E E NE E E 2 Safety instructions esseeseeseeseesseseesecrserserseeseeseesseeseesesseeseescessesseeseeseeseeseesseesesee PL E O ea E E E A EE 2 2 EIEI a E A AEE AET E A E E A A AE 2 5 Emergency ROCCO SS sarcosracnienova aniar a E S 24 Recycling OIA USL CNOA eapsette ce cace cs Sete E E E Ei 2 5 Declaration of Hazardous Substances DOHS sssssssssssssssssssssssrsrsressssssssssesrrreesessssssssrereresesssss 3 WASEPUNIONC GOSCEIDTION sessccssssunununenentiesirsa nin al MAI AUC NO aaee EESE ENOR tenia ae 5 2 FEU E OVET ION enen 3 3 Illustration of the instrument s sesssesessssssesssssssssssssssssssssessestrettetrereereerensnsssssnssssssssrsrestrerrrrerrrereee 34 WAST VSN AAAS OSC ON egissent 35 nstrumentsoftware description seirian R RE ER Jal Description ofthe Capture tab essasianninsasiman n n 3 5 2 Description of the Library tab as cies acca scutes stcesessnadta ansageaeeacaada hawiaevideconseavess apace 3 5 3 Description of the image view sccccsccchscsescecesssectcncstsvasintusetesivusisctedesisessstcacentsvciowssinsctedeasbiarieen 3 5 4 Description of the analysis workflow eccssssssssssesssessssssessssessssecsssesssecsssssssess
43. Imager 600 Operating Instructions 29 0645 17 AC 83 5 Operation 5 2 Turn on the instrument Step Action 4 Select the icon for Amersham Imager 600 on the desktop screen of the connected tablet computer Result 1 A welcome screen appears Welcome to Amersham Imager 600 2 The following screen will be displayed Amersham Imager 600 84 Amersham Imager 600 Operating Instructions 29 0645 17 AC Step Action 5 Operation 5 2 Turn on the instrument 5 Select the Continue button Result The following screen will be displayed wis Cheruluminescence Capture Library Chemiluminescence lt Colorimetric marker i Colorimetric 4 Fluorescence CCD status Wi lower position 2013 November 18 14 27 6 Wait for the CCD status to turn green Capture e Colorimetric 4 Fluorescence wit Chemiluminesc Library Chemiluminescence Colorimetric marker ence Lower position 2013 November 11 10 30 Result Image capturing can begin Amersham Imager 600 Operating Instructions 29 0645 17 AC 85 5 Operation 5 3 Select tray 5 3 Select tray The following table describes the suitable tray and accessory for each capturing method Note Use the correct tray to the selected exposure for optimal image quality Image capturing method Sample type Tray and accessory Available in Chemiluminescence Membrane Black Tray configura tion 600
44. Operating Instructions 29 0645 17 AC 133 5 Operation 5 8 Analyze the image Lane Creation E T Number of lanes 4 234567 GCE Lane width e Move corner point 3 NA Next W Background Bands MW Normalization Summary 8 1 Function Illustration 1 The current step in the analysis workflow highlighted 2 Back button Use the button to close the analysis workflow 134 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image Function Illustration Image view buttons Contrast button Use to adjust the contrast of the dis played image Press a finger on the screen and make a horizontal motion with your finger or movement of the cursor to adjust the brightness A vertical motion will adjust the contrast Lane profile button Use the button to view a selected lane profile Use the drop down list to select which lane to view Image view button Use the button to display the image Zoom buttons Use the button to zoom in on or zoom out of the image Navigation arrow buttons Use the button to move the image on the screen The automatically created lane grid and lane numbers The displayed image tab highlighted with a blue frame Options and steps associated to the current step in the analysis Help icon Use to reveal additional information on the selected step in the analysis Next button Use to ope
45. RNING Power cord Only use power cords with approved plugs delivered or approved by GE WARNING Protective ground Amersham Imager 600 must always be connected to a grounded power outlet CAUTION Amersham Imager 600 must be secured before transport or re location Contact GE for instructions before transporting the instru ment gt PrP gt D Amersham Imager 600 Operating Instructions 29 0645 17 AC 15 2 Safety instructions 2 1 Safety precautions O NOTICE Do not connect telephone lines to the Ethernet port Only the IEC 60950 I VL 60950 1 standard non shielded cables are appropriate for connection to this connector NOTICE Only use IEC 60950 1 certified equipment and cables to connect to the instrument Operation WARNING Do not use the instrument within or near a sink or in humid above 70 RH or dusty environments This can result in fire or electric shock WARNING In case of thunder do not touch the power supply plug as this can result in electric shock CAUTION Always use appropriate Personal Protective Equipment PPE during operation and maintenance of Amersham Imager 600 e Protective eyewear e Protective gloves e Protective footware preferably with steel lining 16 CAUTION Reagents used to prepare the sample should be used in accordance with the manufacturer instructions Amersham Imag
46. Serial number Software version 0 4 4 Firmware version 291 FPGA Version 2557891634 Software update Export log Ready Lower position 2013 November 6 10 08 e Instrument serial number e Software version e Firmware version e FPGA version The view also displays buttons for software update and export log functions Update software Follow the instructions below to update the software for Amersham Imager 600 Step Action 1 Connect a USB flash drive containing the update file provided by GE stored at the root level of the USB flash drive 2 Select the Software update button 3 Confirm USB insertion by selecting the OK button 4 Select the Yes button to update the software 70 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 3 System settings 4 3 4 Service Step Action 5 To return to the Settings window select the OK button Export log Follow the instructions below to export a log from Amersham Imager 600 Step Action 1 Select the Export log button Result A text file will be created on the instrument containing the export log file 2 Insert a USB flash drive and confirm insertion by selecting the OK button Result The export log file will be transferred to the USB flash drive 3 To return to the Settings view select the OK button Amersham Imager 600 Operating Instructions 29 0645 17 AC 71 4 Installation 4 4 Network 44 Network Introduction Via
47. a network connection you can save image files to a network location access files stored on a network location from the instrument and copy and delete image files from a computer This section contains information on network setup options virus protection network settings and file access In this section Section See page 4 4 1 Network setup 73 4 4 2 Network access from instrument 77 4 4 3 Instrument access from network 79 72 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 4 Network 4 4 1 Network setup 4 4 1 Network setup Virus protection The technical solution for Amersham Imager 600 protects it from virus transfer from in fected USB flash drives Each restart of the instrument resets the software to its original installation Note Ifyou for any reason suspect that the instrument has been infected by a computer virus restart the instrument as soon as possible Network connection options NOTICE Do not connect telephone lines to the Ethernet port Only the IEC 60950 I VL 60950 1 standard non shielded cables are appropriate for connection to this connector Connection to a network enables the instrument to access files from and to save files to a network location There are two options for network setup 1 DHCP 2 Static IP Note Ifa DHCP network is used the IP address will be assigned automatically by the DHCP server If a Static IP network is used the networ
48. ackground Background method Intensity 30000 None 25000 Rubber band 20000 z Minimum profile 15000 Sol Rolling ball 5000 Radius 5 10 15 20 5 3 35 30 Distance mm 0 1 Q Q Subtract m A Lanes Background Bands MW Normalization Summary 4 To view the result in any lane select the lane number to view in the drop down menu 5 When you are done select the Next button or select the Bands button in the workflow The Previous button will bring you back to the previous step 140 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 3 Band detection 5 8 3 Band detection Description This is the third step of the analysis workflow The options for band detection are present ed next to the image Bands can be detected automatically and or manually However manual bands can only be added in single channel view Bands modified manually are highlighted in the image The bands resulting from multichannel imaging are color marked in the image or lane profile according to the detection channel used For example a band detected in the green channel is displayed with a green frame etc Detecting bands Follow the instructions below to detect bands Step Action 1 Select the Auto detect button to detect the bands automatically W s 123 6789 Senatity 7 _ Eat ew etual bend e r Postion A A
49. al de tergent or 70 ethanol then wipe with a new clean dry cloth To clean the interior of the instrument use a soft cloth slightly dampened with distilled water or 70 ethanol Make sure to wipe the interior dry Do not use any neutral domestic detergent as the detergent residue may fluoresce when an image is captured Amersham Imager 600 Operating Instructions 29 0645 17 AC 151 6 Maintenance 6 2 Accessories 6 2 Accessories Introduction Amersham Imager 600 accessories must be cleaned after use This section describes recommended cleaning agents and how to clean the accessories Recommended cleaning agents NOTICE Cleaning agents should be for biochemical use to avoid that residues fluoresce when images are captured The accessories may be washed with water and one of the following e 70 ethanol e Mild neutral detergent e lsopropanol e 6 H0 Cleaning sample trays white insert and diffuser board CAUTION Wear gloves while cleaning There may be residues of hazardous chemicals NOTICE Do not use organic solvents These may damage the surface 152 Amersham Imager 600 Operating Instructions 29 0645 17 AC 6 Maintenance 6 2 Accessories NOTICE Do not use abrasive cleaning materials such as a scouring pad for cleaning This may scratch the surface After cleaning trays inserts and or diffuser boards wash the accessory in water using a
50. ata and damage the Compact flash drive Only turn off power in an emergency situation NOTICE Do not open the instrument door during exposure when the RUN light indicates operation This will stop the exposure and data will not be saved NOTICE Transferring large amounts of files to a connected computer during operation can impair the system s performance 18 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 1 Safety precautions NOTICE Do not touch the light source windows as scratches or dirt may impair performance NOTICE The tablet computer cannot be recharged with this instrument NOTICE When connecting an external monitor to the system use a monitor with SVGA resolution With a low resolution monitor operation buttons cannot be seen Maintenance WARNING Electrical shock hazard All instrument repairs or modifications should be performed by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation WARNING Do not use excessive amounts of liquids for cleaning the Amersham Imager 600 instrument This may result in instrument malfunction or electric shock Amersham Imager 600 Operating Instructions 29 0645 17 AC 19 2 Safety instructions 2 1 Safety precautions CAUTION Always use appropriate Personal Protective Equi
51. capture 107 89 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 1 Available methods 5 5 1 Available methods This section describes how to capture an image The table below describes the available methods for each Amersham Imager 600 con figuration Chemiluminescence Chemiluminescence with colorimetric marker Colorimetric Epi illumination Colorimetric Trans illumination Fluorescence UV trans illumination Fluorescence Blue Green Red Epi illumination single channel or multichannnel Fluorescence Blue Green Red Epi illumination with colorimetric marker single channel NOTICE Do not open the instrument door during exposure when the RUN light indicates operation This will stop the exposure and data will not be saved 90 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture 5 5 2 Chemiluminescence capture Introduction The chemiluminescence image capturing method is available in all configurations of Amersham Imager 600 The instrument captures the light which is emitted during the chemical reaction on the membrane When a colorimetric marker is present in the sample an additional color image can be captured using a white light source Chemiluminescence image capturing is performed on one to four membranes placed on a Black Tray Chemi
52. ces 6 Safety precautions 13 Installation precautions 15 Mainenance precautions 19 Operation precautions 16 Personal protection 14 Safety precautionsGeneral precautions General precautions 13 Sample and tray combina tions 86 Save location 122 Save the image 121 Settings Date and time 65 Maintenance 70 Save location 122 167 Index Settings options 53 Single channel imaging 108 112 Site requirements 61 164 Software File formats 126 Keypad 54 Specifications 163 Start screen 40 T Tool Web 79 Tools Contrast tool 119 Display tool 118 Intensity tool 120 Zoom tool 118 Troubleshooting 155 Two channel imaging 108 Typographical conventions 7 U User information important 6 V Virus protection 73 W Web tool 79 Windows 7 77 Z Zoom tool 118 Amersham Imager 600 Operating Instructions 29 0645 17 AC Page intentionally left blank For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative_imaging GE and GE monogram are trademarks of General Electric Company Amersham Cy and ECL Plex are trademarks of General Electric Company Safari is a trademark of Apple Inc SYBR is a trademark of Life Technologies Corporation Windows 7 is a registered trademark of Microsoft Corporation 2014 General Electric Company All rights reserved F
53. ck vents and ensure that they are kept free of dust and dirt Blockage can cause overheating and malfunctioning To ensure adequate cooling ensure there is at least 40 cm of free space in front of the instrument and at least 10 cm on all other sides to walls or other equipment Personal protection WARNING Hazardous substances When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of the system 14 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 1 Safety precautions CAUTION The instrument is equipped with a safety interlock If the interlock is out of order or has been tampered with UV and visible light may be emitted which may cause skin burn and impair vision Do not open the Instrument door during operation when the RUN indicator is lit If the door is opened and the instrument keeps run ning turn off the instrument immediately and contact GE service CAUTION Do not touch the UV unit after UV exposure There is a risk of skin burn Installation WARNING As the equipment is heavy contact a GE service person when moving or relocating the main body of the equipment If the equipment drops it may be broken or you may get seriously injured if it drops on your foot WA
54. con e 8 Select the Save locations button 9 Select the Add new button 10 Browse to the location or type the name of the computer in the field Loca tion Example HXX DTKJ8Q9 clients region division compa ny com AmershamImager600 Amersham Imager 600 Operating Instructions 29 0645 17 AC 77 4 Installation 4 4 Network 4 4 2 Network access from instrument Step Action 11 Select OK Result This will allow for sharing files from the computer to the instrument and from the instrument to the computer for users Note If password protected sharing is turned on you must have a user account and password on your computer for full access to shared items Password protected sharing is located in the Control panel under Advanced sharing settings Pass word protection is turned on by default 78 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 4 Network 4 4 3 Instrument access from network 4 4 3 Instrument access from network Amersham Imager 600 is equipped with a web tool which allows instrument access from a network The web tool can be used to copy or delete files on the instrument from a remote location To be able to access Amersham Imager 600 web tool the following conditions must be fulfilled e The instrument and the computer must be connected through a local network e The instrument s IP address must be known Your instrument s IP address is found in the Network view Open th
55. d If an error code is displayed make a note of it and contact your GE representative 156 Amersham Imager 600 Operating Instructions 29 0645 17 AC The instrument indicator LED lights do not light during startup diagnosis a pow er cable installation failure has occurred 7 Troubleshooting 7 1 General problems Connect the power cable correctly Operation is not stopped when the door is opened The light source is lit or the motor is run ning when the door to the instrument is open an interlock failure has occurred Turn off the instrument s Power switch immediately and contact your GE repre sentative Other hardware problems The door to the instrument cannot be opened or closed or the door cannot be locked because a foreign object is present in the locking section or the door sensor Remove the foreign object The door to the instrument cannot be opened or closed or the door cannot be locked and no foreign object is present The locking section is damaged Contact your GE representative the instrument cannot be cooled down properly The ambient air temperature is too high Lower the room temperature to 28 C or lower The instrument does not appear to re spond to software control Amersham Imager 600 Operating Instructions 29 0645 17 AC The instrument is not connected to the tablet computer Use the flash indicator tool to identify which instrument is connected to a cer tain
56. e Marker A jog composite image of chemiluminescence and colorimetric data in color and with set contrast Note The composite image is displayed if both tabs are selected simultaneously e A jpg image of the Marker image in color and with set contrast for presentation purposes Note The jpg image is included in the folder but it is not displayed on the screen Colorimetric A folder containing the following files e Sample A tif in gray scale for quantifica tion of colorimetric data e Color A jpg in color and with set contrast for presentation purposes Amersham Imager 600 Operating Instructions 29 0645 17 AC 127 5 Operation 5 7 Save and manage image files 5 7 2 Save images Imaging method Fluorescence Single channel imaging File format of saved image A folder containing the following files Blue Green Red A tif in gray scale for quantification of fluorescence data A jpg in gray scale and with set contrast for presentation purposes Note The jpg image is included in the folder but it is not displayed on the screen Fluorescence Single channel imaging with colorimetric marker A folder containing the following files Sample A tifin gray scale for quantifica tion of fluorescence data Marker A tif in gray scale for quantifica tion of colorimetric data Note Both the above files have the same pixel resolution Sample Marker A jog composite i
57. e jpg Gray scale jpg Amersham Imager 600 Operating Instructions 29 0645 17 AC 163 8 Specifications Parameter Emission filters Dimensions and weight Unit Amersham Imager 600 Power supply Parameter Input voltage Cy2 525BP20 Cy3 EtBr 605BP40 Cy5 705BP40 Dimensions Weight 360 W x 785 H x 485 D mm 100 to 240 V Voltage variation Frequency 10 50 60 Hz Max power Environmental conditions 250 W The following table describes the environmental requirements for Amersham Imager 600 164 CAUTION Do not use the instrument in a room with a temperature above 28 C Higher temperatures does not allow the CCD to cool down properly to 25 C Amersham Imager 600 Operating Instructions 29 0645 17 AC Parameter Free space required around Amersham Imager 600 8 Specifications Condition Front of instrument the operation panel side 40 cm Right 10 cm Left 10 cm Rear 10 cm Top 10 cm Placement A stable laboratory bench with a load capacity of 980 N 100 kg or higher The bench must be level within 2 two degrees Other conditions 1 Take into consideration the work flow and ancillary facilities when planning the installation Any required construction and electricity air conditioning work must be completed prior to installation It is not desirable to have a heat source near the Amersham
58. e view by selecting the settings icon in the top right hand corner and then select the Network button in the Settings view Note DHCP networks has a dynamic IP address assignment therefore the IP address may vary between connections When using a DHCP network connection check regularly that the IP address is still accurate If the IP address has changed use the new IP address in the Network view Access the web tool Follow the steps below to set up and access files via the web tool Step Action 1 Open the computer s web browser Z Type the instrument s IP address in the browser address field and select enter Example http 192 168 0 25 8080 Result A page asking for password is displayed E Amersham Imager 600 web tool lagin Amersham Imager 600 Operating Instructions 29 0645 17 AC 79 4 Installation 4 4 Network 4 4 3 Instrument access from network Step Action 3 Type the default password AI 600user and select Login Result This opens the Amersham Imager 600 web tool page Amersham Imager 600 web tool Amenia Image 600 web tool aloes you lo copy ood delete fen stoned cn Amentam Imager 00 hanco zabon OF chata ihe eng Mes the fes wil be sect none archiva Me fo aloza Image list Dais from Rayword Search ih aipate Cher T309S0_ 161545 Ch 201309148 anne corde 130919_ 188052 Fi 20720018 anne papardo i Soe ITIO Pipi tf 207130918 anne peper201 0818 Trga A 20130818 ceni 120130010 16 _F Copy
59. electing the Yes button Edit save location This table describes how to edit a location for files saving Step Action 1 Select the settings icon in the top right hand corner of the screen 2 Select the Save locations button 3 Select the Edit button 4 If desired edit the name for the save location in the Name field 5 If desired edit the folder for the save location by selecting the Browse button next to the Location field Note The network settings must be properly configured before a network folder can be added as a location for saving files For information on how to configure a network see Section 4 4 Network on page 72 6 To save the changes select the OK button 124 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 7 Save and manage image files 5 7 2 Save images 5 7 2 Save images Saving the image Follow the instructions below to save the image s Step Action 1 Select Save to save the image or images i rags Linear Log Result The Save view opens The automatically generated image name s are displayed in the lower part of the view Where to save the file On instrament Optional image name prefix Comment image names 20131118_144726_Ch 20131196_144726_Ch Marker Note If a split image is saved then the screen will display the split suffix Example Wither ti dare thea Sie Cen airar l Ophonal imagE name prin
60. ent 87 5 5 Capture an image 89 5 6 View the image 117 5 7 Save and manage image files 121 5 8 Analyze the image 133 5 9 Turn off the instrument 149 Amersham Imager 600 Operating Instructions 29 0645 17 AC 81 5 Operation 5 1 Operation flow chart 5 1 Operation flow chart The general flow of operation is as follows Turn on the instrument Select a tray and place sample Place the tray in the instrument Capture the image Save the image file Analyze the image i 82 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 2 Turn on the instrument 5 2 Turn on the instrument The following section describes the startup process Note Ifusing a wired external monitor or touch screen it must be connected to the in strument before start up Step Action 1 Turn on power to the instrument by pressing the Power switch on the bottom right side of the instrument to the I position e l 2 Press the On Off button on the front of the instrument to activate it 4 Result The instrument s self diagnostic process will initialize Note The self diagnostic process will normally take approximately 5 minutes Connection to a network may take longer 3 If relevant connect a USB flash drive to the instrument for file storage The USB flash driver is automatically installed when the USB flash drive is connected to Amersham Imager 600 Note Password protected USB flash drives are not supported Amersham
61. er 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 1 Safety precautions CAUTION Do not lean on the sample door CAUTION Use the handle to open or close the instrument door CAUTION Do not place heavy objects on top of the instrument CAUTION Handle sample trays and tablet computers with care to avoid dropping them CAUTION Do not touch the light source in the instrument The light source may be hot CAUTION Do not leave samples in the instrument after exposure If left these may degrade and cause damage to the instrument CAUTION Do not disable the interlock of the equipment as UV light can be emitted if the door is opened NOTICE Do not use USB devices other than those specified This can cause instrument malfunction OP PP PEPE amp Amersham Imager 600 Operating Instructions 29 0645 17 AC 17 2 Safety instructions 2 1 Safety precautions NOTICE Do not use USB ports for recharging external devices This can cause instrument malfunction NOTICE Connection of multiple computers to one instrument may impair performance Simultaneous use can cause loss of data NOTICE Avoid connecting additional accessories such as computers monitors or keyboards to the instrument during operation as this can stop the exposure or cause loss of data NOTICE Do not turn off power during operation as this can cause loss of d
62. es and UV trans illu minator Color images of colorimetrically stained gels or membranes obtained by white light illumination Accurate quantification of stained gels using white trans illumination Intuitive design with easy to use image analysis workflow Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 3 Illustration of the instrument 3 3 Illustration of the instrument The Amersham Imager 600 instrument is operated from a wireless connected tablet computer It may also be operated from a wired touch screen or a monitor with mouse The illustration below displays Amersham Imager 600 setup with tablet computer Amersham Imager 600 Operating Instructions 29 0645 17 AC 33 3 Instrument description 3 4 Instrument hardware description 3 4 Instrument hardware description Main components in each configuration Amersham Imager 600 is available in four different configurations Different parts and functions of Amersham Imager 600 are available depending on configuration The table below describes the functions and components of the different instrument configurations Functions Amersham Amersham Amersham Amersham Imager 600 Imager 600 Imager 600 Imager 600 29 0834 61 UV QC RGB 29 0834 63 29 0834 64 29 0834 67 Control Software for image capture and analysis Chemiluminescence CCD camera head including lens Epi White light source Filter changer Trans UV l
63. f this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the inter ference at his own expense FCC WARNING Changes or modifications not expressly approved by the party respon sible for compliance could void the user s authority to operate the equipment Amersham Imager 600 Operating Instructions 29 0645 17 AC 9 1 Introduction 1 2 Regulatory information CE marking The CE marking and the corresponding EC Declaration of Conformity is valid for the in strument when it is e used asa stand alone unit or e connected to other products recommended or described in the user documentation and e used in the same state as it was delivered from GE except for alterations described in the user documentation Environmental Conformity Directive 2011 65 EU Annex II Restriction of Hazardous Substances ROHS Directive 2012 19 EU Waste Electrical and Electronic Equipment WEEE Directive ACPEIP Administration on the Control of Pollution Caused by Elec tronic Information Products China Restriction of Hazardous Substances ROHS Regulatory compliance of connected equipment Any equipment connected to Amersham Imager 600 should meet the safety require ments of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within EU con nected equipment must be CE marked Light sources The safety of the LED light sources in this instrume
64. for chemiluminescence capturing are displayed to the right on the screen If your sample contains a colorimetric marker then check the check box Colorimetric marker Select Manual exposure in the exposure drop down list rary ommur a nasaat Colorimetne Auto Serv auto Manual incremertal 44 Fucrescerce Exposuwre Maruca Exposure ume 24s CCO status A Lower position 2013 November 11 10 39 To change the exposure time select the exposure time field Result The Set exposure time view opens Library Sel exter bme You can vsa both the mamenc keys and the buttons to set tiene Mines Seconds iit s ok 4 o te E CCO sais A Lower postion 2013 Movember 11 10 40 Enter the new time and select Set to return to the previous view For detailed instructions see Section 3 5 5 Description of settings views on page 53 Select Start and wait for the exposure to finish Amersham Imager 600 Operating Instructions 29 0645 17 AC Step Action 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture 7 When the exposure is finished the image view appears displaying the im age s Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126 No colorimet ric marker was used The resulting image is displayed in gray scale G as Log Colorimetric marker was used
65. ge is inadequate af ter automatic exposure or a known exposure time is required Select Manual exposure and set the exposure time man ually A sequence of images is required will not be saved Select Incremental exposure and set the number of repe titions and interval time between exposures to capture sequential images Note Do not open the door during exposure as this will stop the exposure and the data Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Chemiluminescence with automatic exposure time Follow the instructions below to perform chemiluminescence capture using automatic exposure time Step Action 1 In the Capture tab select Chemiluminescence Result The options available for Chemiluminescence capturing are displayed to the right on the screen Automatic exposure is selected by default and the Exposure options field displays Auto Capture Library e wi Chemiluminescence Colorimetric 4 Fluorescence CCD status fH Lower position 2013 November 11 10 30 fs If your sample contains a colorimetric marker then select the check box Colorimetric marker V Colorimetric marker 3 Select Start Result The exposure will begin following a pre exposure A progress bar appears on the screen indicating the time remaining of the imaging process the exposure selected and the actual exposure time
66. ght buttons to rotate rae the image Rotation changes will be saved in the image file Split image Use the Split image buttons to split the image into separate a image files The selected split option is marked and red Picci Meds lines illustrate the split in the image Split changes will be 2 46 saved in the image file Open the Zoom tool by selecting the zoom button in the image tools menu Use the plus or minus buttons to zoom in or out in the im age When zooming in arrows appear in the image view Use the arrows or point to the image and drag to position the image on the screen If a different tool is selected when the image is zoomed in the selected zoom will be kept until saved Use the Reset button to return to the original zoom view 118 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 6 View the image Description Illustration Open the Contrast tool by selecting the contrast button in the image tools menu The contrast tool allows for modifications of image contrast Contrast and brightness and displays the results as modifications are made The software will automatically adjust the contrast if you select the Auto button Image histogram A histogram is displayed illustrating the intensity distribu tion plotting the number of pixels for each intensity value in the captured image The horizontal axis represents inten sity variations The vertical axis represent
67. ham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS 2 5 Declaration of Hazardous Substances DoHS Introduction The following product pollution control information is provided according to SJ T11364 2006 Marking for Control of Pollution caused by Electronic Information Products ARHHSI T11364 2006 BFS mm SRE MRR HRMS AAR Bel JAER Symbols used in pollution control label BF ST on RE Bl ne AB Meaning This symbol indicates the product contains hazardous materials in ex cess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Sub stances in Electronic Information Products The number in the symbol is the Environment friendly Use Period EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating conditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain the declared EFUP the product shall be operated normally according to the instructions and environmental conditions as defined in the product manual and periodic maintenance schedules specified in Product Maintenance Procedures shall be followed st
68. he Amersham Imager 600 icon has been Connect to the same network as accidently removed from the tablet Amersham Imager 600 Open the Safari computer web browser and type in the IP address of the instrument in the address field Once the instrument connect screen Is shown add this page to the home screen of the tablet computer by clicking on the square with an upward arrow next to the address field in the browser Tip Ifa USB dongle is used on the camera the default IP address is 192 168 173 1 162 Amersham Imager 600 Operating Instructions 29 0645 17 AC 8 Specifications About this chapter 8 Specifications This chapter contains the technical specifications for the Amersham Imager 600 system Imaging specifications Parameter Image sensor Peltier cooled 3 2 megapixel CCD Image sensor temperature 25C Cooling down time lt 5 minutes Lens F0 85 43 mm Light source Blue Epi light 460 nm Green Epi light 520 nm Red Epi light 630 nm UV transillumination light 312 nm White Epi light 470 to 635 nm White transillumination light 470 to 635 nm Operation Fully automated auto exposure no focus or other adjustment or calibrations needed Maximum sample size 160 X 220 mm Greyscale Exposure time 65 536 levels 16 bit 1 10 seconds to 1 hour Dynamic range 4 8 orders of magnitude Image output Gray scale 16 bit tif Color imag
69. he file Select Done to save the changes For detailed information on how to use the keypad see Keypad view on page 54 Amersham Imager 600 Operating Instructions 29 0645 17 AC 131 5 Operation 5 7 Save and manage image files 5 7 3 File manage options Open files Follow the instruction to open files Step Action 1 Select the file to open 2 Select the Open button to open the file 3 If desired use the Image tools to adjust the view of the image For detailed information on the Image tools see Image tools on page 118 4 When finished select the Done button to go back to the Library tab 132 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 Analyze the image Introduction This section describes how to analyze the image using the analysis tool The analysis workflow in Amersham Imager 600 allows you to perform a step by step analysis of the image data and to save the analysis results The workflow is performed in five steps and the final result is presented in a summary You can analyze a newly captured image or analyze a previously saved image Select the Analyze button in the image view to open the analysis workflow view Sample Marker i Display Invert Rotate Buen 90 3 90 Split image Exposure times 7 88 Print Done The following illustration and table describe the start view of the analysis workflow Amersham Imager 600
70. he typed text 54 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 5 Description of settings views Function Done button Use the button to save the changes Space button Use the button to create a space Cancel button Use the button to return to the previous window without saving the changes abc button Use the button to change to the lower case letters keypad 123 button Use the button to change to the numeric and symbol keypad Note Use abc or the ABC button to return to the alphabetic keypad The Set exposure time view appears when you select Manual or Incremental exposure inthe drop down menu and then select the Exposure time button or Interval time button It allows you to set the exposure time manually The illustration below shows the main components of the Set exposure time view Capture Library e Set exposure time You can use both the numeric keys and ths buttons to set time 3 4 n see 1 10 eT 1 2 a 5 6 0 00 1 ii z 7 8 9 lt 0 gt 5 _ Cancel Set 1 EEE CCD status Lower position 2013 November 11 10 23 Note The time displayed in the Set exposure time window is by default the exposure time used during the last capture The time can be set from 0 1 seconds up to 60 minutes Part Function 1 Cancel button Use to return to the Capture tab witho
71. i Ready Blower positis 2013 Gctober 11 1323 3 Select the Add new button Result The following screen appears Capture Library Add a new save location Name Make sure to use a name that is easy to understand Locahon Browse to network folder or input path of network folder Ready E Lower postion 2013 October 11 13 26 4 Select the Name field and use the keypad to type a name for the new loca tion 5 Select the Browse button next to the Location field Note The network settings need to be properly configured before a network folder can be added as a location for saving files For information on how to configure a network see Section 4 4 Network on page 72 6 In the Choose save location view mark the network folder you wish to add and select the Use this location button 7 Ensure that the path to this location appears in the Location field 8 Select the OK button to save the added location The Cancel button will leave the view without saving changes Amersham Imager 600 Operating Instructions 29 0645 17 AC 123 5 Operation 5 7 Save and manage image files 5 7 1 Save locations Remove save location Follow the instructions below to remove a location for files saving Step Action 1 Select the settings icon in the top right hand corner of the screen 2 Select the Save locations button 3 Select the location you wish to remove from the list 4 Select the Remove button 5 Confirm removal by s
72. ight source Trans White light source Blue Green and Red Epi light source 34 Amersham Imager 600 Operating Instructions 29 0645 17 AC Amersham Imager 600 exterior 3 Instrument description 3 4 Instrument hardware description The illustration below shows the main exterior hardware components of the instrument USB port 2 On Off button and LED indicators O AFH FHH AREENA Se bn Description A USB 2 0 port for connection of USB flash drive Note Password protected USB flash drives are not supported On Off button Press the button to turn on power Press again to turn off the power Power The LED is lit white when the instru ment is powered on Run The LED is lit blue when the instrument is operating or if the Instrument indicator is selected Error The LED is lit red when the instrument is malfunctioning Amersham Imager 600 Operating Instructions 29 0645 17 AC 55 3 Instrument description 3 4 Instrument hardware description Part Name Description Tablet computer dock The dock can hold tablet computers of the following dimensions and weight Width 222 to 242 mm Height 183 5 to 186 mm Depth 9 5 mm or less Weight 750 g or less Note Some tablet computers cannot fit due to the shape of corners Note The tablet computer cannot be recharged on the instrument Door Instrument door Power switch Toggle switch I P
73. ilters Please contact your GE representative for more details as the customized methods require Service Fluorescence Trans UV 114 Capturing with the Fluorescence image capturing method option Trans UV is performed using the UV Trans Tray transparent tray and a gel sheet Fluorescence Trans UV sample preparation Follow the instructions below to prepare the sample Step Action 1 Cut out a gel sheet slightly larger than the sample size Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Step Action 2 Place the gel sheet on the UV Trans Tray transparent tray 4 Proceed with the capturing Note After use wash the gel sheet with mild detergent rinse with water then dry well A gel sheet can be reused about 20 times Follow the instructions below to perform fluorescence capture using Fluorescence Trans UV Step Action 1 In the Capture tab select Fluorescence The options available for Fluores cence capturing are displayed to the right on the screen Z Choose the Trans UV radio button a Select an exposure option in the drop down menu e Auto or e Semi Auto or e Manual enter the exposure time and press Set Amersham Imager 600 Operating Instructions 29 0645 17 AC 115 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Step Action 4 If Auto or Manual exposure was selected select Start Result
74. ination wit Chemiluminescence Trans illumination quantitative analysis of gels F Colorimetric 44 Fluorescence CCD status Lower position _ 2013 November 11 10 45 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 3 Colorimetric capture Step Action 2 Choose Epi illumination or Trans illumination as preferred Note For Colorimetric Epi illumination capture position the gel so that the lanes of the gel are parallel to the long side of the tray This is important to avoid artefacts in the image Note For Trans illumination place a diffuser ai a board at the bottom of the instrument n with the matte side up before capturing 17 3 Select Start Result A progress bar appears on the screen indicating the time remaining of the imaging process and the exposure time Colorimetric Remaining time Automatic exposure in progress 1 min Calculating exposure time Cance CCD status amp Lower position 2013 November 11 10 56 Amersham Imager 600 Operating Instructions 29 0645 17 AC 105 5 Operation 5 5 Capture an image 5 5 3 Colorimetric capture Step Action 4 When the exposure is finished the image view appears displaying the im ages Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126 Select the relevant tab of Sample and Color f
75. irst published Jan 2014 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 0645 17 AC 03 2014 a1687
76. k details have to be entered manually Amersham Imager 600 Operating Instructions 29 0645 17 AC 73 4 Installation 4 4 Network 4 4 1 Network setup Connect to network Follow the instructions below to connect to a Network Step Action i Select the settings icon Result The Settings view will appear Capture Library re Settings System settings Focus Sleep Date amp Time Service Instrument indicator J Show mouse cursor Network settings Network Save locations CCD status M Lower position 2013 November 11 10 12 2 Select the Network button Result The Network view will appear Capture Library eS Network DHCP IP address Static IP Subnet mask Static IP Enter network Gateway settings manually Contact your system Workgroup administrator for proper settings CCD status Wy Lower position 2013 November 11 10 29 5 Select the type of network connection and follow the separate instructions below Connect to a DHCP network Follow the instructions below to connect to a DHCP network 74 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 4 Network 4 4 1 Network setup Step Action i Connect the network cable to the Ethernet port on Amersham Imager 600 2 Check the DHCP radio button in the Network window Capture Library ce Network DHCP IP address 197 168 0 17 static IP Subnet mask 255 255 255 0 DHEP IP address will be Gateway 192 168 0 1 assigned
77. kes even if the other environmental require ments have been met 4 Donotinstall the equipment near a window Avoid direct sunlight Ensure blinds are attached to nearby windows 5 Donot place objects near the power outlet to ensure easy access to the power cord for disconnection in case of emergency Amersham Imager 600 Operating Instructions 29 0645 17 AC 61 4 Installation 4 1 Site requirements Parameter Operating temperature humidity conditions Condition Temperature 18 C to 28 C with temperature fluctuation below 10 C per hour or lower Humidity 20 to 70 RH no dew condensation Note When the above conditions cannot be satisfied take appropriate actions Transportation storage conditions Temperature 25 C to 70 C Humidity 5 to 95 RH no dew condensation Note When the above conditions cannot be satisfied take appropriate actions Installation location conditions 1 Do not install the instrument where the temperature can vary widely with temperature fluctuation above 10 C per hour Do not install the instrument near a heat source such as a radi ator Do not install the instrument where It may get wet or flooded Do not install the instrument where it may be exposed to corro Sive gas Do not install the instrument in a dusty environment Do not install the instrument in a place constantly or excessively exposed to vibration or impacts 7 Donotinstall the instr
78. luminescence image capturing of samples with colorimetric marker is performed on one to four membranes placed on a Black Tray with White Insert There are two alternative tray positions 1 Upper tray position Use the upper tray position for a smaller field of view of a single membrane and shorter exposure time The field of view is 8 x 11 cm 2 Lower tray position Use the lower tray position for a larger field of view for one to four membranes The field of view is 16 x 22 cm Amersham Imager 600 Operating Instructions 29 0645 17 AC 91 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Chemiluminescence exposure options 92 An optimal dynamic range is required There are four exposure options for Chemiluminescence capturing The table below suggests the options to use in different situations Select Auto for automatic exposure A short pre exposure is performed to determine the signal intensity The system will use this information to calculate which exposure time will give the highest possible signal below saturation to enable accurate quantification of the sample A specific area of a sample is of interest Select Semi automatic A short pre exposure is performed which results in an image This allows you to select the area of the sample image to focus on The software automatically calculates the optimal exposure time for the specified area The intensity of the ima
79. mage of fluorescence and colorimetric data in color and with set contrast Note The composite image is displayed if both tabs are selected simultaneously A jpg of the Marker image in color and with set contrast for presentation purpos es Note The jpg image is included in the folder but it is not displayed on the screen 128 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 7 Save and manage image files 5 7 2 Save images Imaging method File format of saved image Fluorescence A folder containing the following files Multichannel imaging e Blue A tif in gray scale for quantification of fluorescence data Green A tif in gray scale for quantifica tion of fluorescence data Red A tif in gray scale for quantification of fluorescence data Note The number of tif files created correspond to the number of selected detection chan nels Blue Green Red A jpg composite im age of fluorescence data in color and with set contrast Note The composite image is displayed if two or three tabs are selected simultaneously Amersham Imager 600 Operating Instructions 29 0645 17 AC 129 5 Operation 5 7 Save and manage image files 5 7 3 File manage options 5 7 3 File manage options Select and deselect all The Select all and Deselect all buttons are used to select or deselect all of the files in the library Delete files Follow the instruction to delete files
80. ment Focus button Use to adjust focus Sleep button Use to set the time when the automatic CCD cooling shall stop after periods of non use Amersham Imager 600 Operating Instructions 29 0645 17 AC 53 3 Instrument description 3 5 Instrument software description 3 5 5 Description of settings views Part Function 8 Service button Use to view instrument serial number version number and for accessing Software update and Export log Save locations button Use to add new edit or remove network locations for image file storage Keypad view The keypad appears when you select text fields where typing is required Examples of text fields are Search Optional image name prefix Comment or Rename The following illustration and table show the main components of the keypad The image to the left shows the alphabetic view of the keypad with lower case letters The image to the right shows the numeric and symbol view of the keypad 1 1 Function al The text field where the typed text is displayed 2 Clear button Use the button to remove all typed text in the text field 3 Keyboard area Use the keyboard to type text 4 Delete button Use the button to delete one letter at a time 5 Enter button Use the button to create a new row 6 Shift button Use the button to change to upper case letters keypad 7 Navigation buttons Use the buttons to navigate back and forward in t
81. mination Epi White 600 600 600 UV QC RGB Trans illumination Gel Trans White Both options use white light imaging sources For Epi illumination the light source is lo cated at the top of the Amersham Imager 600 interior For Trans illumination the sample is illuminated from the light source at the bottom of the instrument interior When using Trans illumination the intensity data is converted to optical density OD values which provides a more quantitative result compared to Epi illumination The images are by default created with automatic exposure time The table below describes details on accessories and tray positions for colorimetric image capturing Epi illumination Tray and accessory Black Tray White In sert Tray position Lower tray position White Lower tray position Trans Tray 103 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 3 Colorimetric capture Tray and accessory Tray position Trans illumina White Lower tray position tion Colorimetric capture Trans Tray Diffuser Board Follow the instructions in the table to perform colorimetric capture Step Action j 104 In the Capture tab select Colorimetric Result The options available for colorimetric capturing are displayed to the right on the screen B Capture Library i ko Colorimetric Epi ilum
82. n overview of the result data from the analysis Use the Save button to save the analysis data together with the image file 52 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 5 Description of settings views 3 5 5 Description of settings views This section describes various settings views and options General setting view The settings options found in the settings view allows you to set specifications for the instrument The settings view is displayed by selecting the settings icon o The illustration below shows the main components of the Settings view For details see Section 4 3 System settings on page 64 6 Capture Library Settings Ses Focus Sleep oT 8 5 Date amp Time Service 4 3 2 instrument indicator h Show mouse cursor Network settings ee Network Save locations ToL CCD status M Lower position 2013 November 11 10 12 Function Back button Use to close the settings view and return to the previous view Network button Use to set up DHCP or Static IP network Show mouse cursor check box Use to display a mouse cursor on a con nected external monitor by checking the check box Instrument indicator button Use to activate the instrument connected to the tablet computer and light the instrument Run LED light Date amp Time button Use to set date and time on the instru
83. n the next step in the analysis workflow Amersham Imager 600 Operating Instructions 29 0645 17 AC 135 5 Operation 5 8 Analyze the image 5 8 1 Lane creation 5 8 1 Lane creation Description This is the first step of the analysis workflow The selected image appears on screen with a grid of automatically created lanes and the button Lanes marked in the workflow The options for lane creation are presented next to the image Use the Lane Creation options to the right to arrange the lane grid to correspond with the lanes in the image Sample Marker LWA Lane Creation Number of lanes 234567 sjt Lane width 70 Move corner point AN lt gt X QQ v i Lanes Ba 2 Zatic Adjust the lane grid 136 Follow the instructions below to adjust the lane grid to match lanes in the image Step Action 1 Select the minus and plus buttons adjacent to Number of lanes to increase or decrease the number of grid lanes to correspond with the number of lanes in the image 2 Select a grid corner circle Result The selected corner circle is highlighted Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 8 Analyze the image 5 8 1 Lane creation Step Action 3 Drag and drop the corner or use the Move corner point arrow buttons to move it to the correct lane position on the displayed image Repeat until all four corners are positioned corresponding to the lanes in
84. nents of the settings view see Section 3 5 5 Description of settings views on page 53 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 3 System settings 4 3 1 Date and time 4 3 1 Date and time Date and time view The following illustration describes the Date amp Time view Capture Library re Date amp Time 2 Date Time Year 2013 Hour 15 Month Oct Minute 08 Day 17 1 ae Cancel Lower position 2013 October 17 15 08 Function Cancel button Use the button to return to the Settings window without saving the changes Date field Use the plus and minus buttons next to Year Month and Day to set values for year month and day Time field Use the plus and minus buttons next to Hour and Minute to set values for hour and minute Ok button Use the button to save the changes to date and time and return to the Settings window Change date To change the date setting on the instrument select the plus or minus buttons next to the Year Month and or Day field to change the value of year month or day 15 Amersham Imager 600 Operating Instructions 29 0645 17 AC 65 4 Installation 4 3 System settings 4 3 1 Date and time Change time To change the time setting on the instrument select the plus or minus buttons next to the Hour or Minute field to change the value of hour or minute 15 Save
85. nsity results in saturation and prevents quan titative analysis Saturation occurs close to 65 500 intensity levels Print image Image files may be printed directly from the result image screen The printing option requires a printer to be connected to the instrument Print using the Print button Contact your GE representative for further details on printer installations 120 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 7 Save and manage image files 5 7 Save and manage image files Introduction After capturing the image s can be saved You can also copy move rename delete search sort and open files If the Amersham Imager 600 instrument is connected to a network you can access and manage files stored on the network location This section describes the locations where you can save files how to save a file the various file formats and the file management options NOTICE Transferring large amounts of files to a connected computer during operation can impair the system s performance Amersham Imager 600 Operating Instructions 29 0645 17 AC 121 5 Operation 5 7 Save and manage image files 5 7 1 Save locations 5 7 1 Save locations Introduction You can save files on several locations e On the instrument Note Amersham Imager 600 is equipped with a flash drive that allows for storage of a finite number of images on the instrument It is necessary to regularly re
86. nt has been assessed by risk analysis in accordance with EN 61010 1 Amersham Imager 600 depending on configuration is equipped with some or all light sources in the table below 10 Amersham Imager 600 Operating Instructions 29 0645 17 AC Light sources Blue LED Wavelength 460 nm Green LED 520 nm Red LED 630 nm White LED 470nm to 635 nm Trans UV illuminator 312 nm Amersham Imager 600 Operating Instructions 29 0645 17 AC 1 Introduction 1 2 Regulatory information 11 2 Safety instructions 2 Safety instructions About this chapter This chapter describes safety precautions safety labels and emergency procedures for the Amersham Imager 600 instrument in addition to information on the safe disposal of the instrument In this chapter Section See page 2 1 Safety precautions 13 2 2 Labels 21 2 3 Emergency procedure l5 2 4 Recycling information 26 2 5 Declaration of Hazardous Substances DoHS 27 12 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 1 Safety precautions 2 1 Safety precautions Introduction The Amersham Imager 600 instrument is powered by mains voltage may be equipped with a UV light source and is used to capture images of samples that may be hazardous Before installing operating or maintaining the instrument you must be aware of the hazards described in the user documentation Follow the instructions provided
87. ntal Note For more information on manual and incremental settings see on page 55 Start button Use the button to start capturing Colorimetric The illustration below shows the specific components of the Capture tab with the Col orimetric image capturing method selected For more details of the general components see Capture view on page 40 Capture Library e Colorimetric Epi illumination wi Chemiluminescence Trans illumination quantitative analysis of gels 1 Ss Colorimetric 4 Fluorescence CCD status Blower position 2013 November 11 10 45 Function Colorimetric image capturing method selected Options area for the colorimetric image capturing method e Epi illumination selected here e Trans illumination 42 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 1 Description of the Capture tab Fluorescence The illustration below shows the specific components of the Capture tab with the Fluo rescence image capturing method Depending on selected options the display changes and unavailable options are hidden greyed out For more details of the general components see Capture view on page 40 Epi RGB Light Filter Capture Library Fluorescence wi Chemiluminescence Colorimetric Custom Trans UV 312 nm EtBr Fluorescence t Expo
88. ons for example USB flash drive or network locations Select the drop down menu for more file location options 3 Search field Use the field to search for specific files 4 Clear button Use the button to clear the search field 5 Scroll buttons and bar Use the bar or buttons to navigate among files in the displayed list of stored files 6 The selected Sort option Select the drop down menu to view the file sorting options e Name name e Date date e Method method 44 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 2 Description of the Library tab Part Function The list displayed of the number of lists available Use the arrows to navi gate between lists The number of selected files Select all Deselect all button Use the buttons to select all files displayed or to deselect a selection of files Delete button Use the button to delete the selected marked file or files Rename button Use the button to rename a saved file Copy Move button Use the button to copy or move selected files Open button Use the button to open the selected file files Amersham Imager 600 Operating Instructions 29 0645 17 AC 45 3 Instrument description 3 5 Instrument software description 3 5 3 Description of the image view 35 5 3 46 Description of the image view After capturing the resulting image or images
89. or the image you wish to display A selected tab is highlighted with blue text and frame Sarie i p EN 5 Select the Save button to save the images For other options after capturing see Options after capturing on page 106 Options after capturing Select one or more of the following actions after capturing e To save the image select Save This will allow you to reopen the image later to use the image tools to perform an analysis or to print the image See Section 5 7 Save and manage image files on page 121 for further instructions e To modify the display settings or view details of the image select the image tools to the right of the image See Image tools on page 118 for further instructions e To analyze the image select Analyze See Section 5 8 Analyze the image on page 133 for further instructions e To print the image select Print e To discard the image without saving select Done and confirm the following dialog 106 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture 5 5 4 Fluorescence capture Introduction Fluorescence image capturing is performed with Epi illumination Blue Green and Red or Trans UV illumination The Epi illumination method allows for both single and multi channel detection Fluorescence capturing is performed using a light source and emission filter The methods available in Amersham Imager 600
90. overview 32 3 3 Illustration of the instrument 33 3 4 Instrument hardware description 34 3 5 Instrument software description 39 3 6 Accessories 57 30 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 1 Introduction 3 1 Introduction Amersham Imager 600 is an imaging instrument designed to identify and quantify pro teins or DNA in gels or membranes A cooled 3 2 megapixel CCD camera is used to capture high resolution digital images of protein and DNA bands in gels and on mem branes obtained by electrophoresis or western blotting separation methods The instru ment can capture images of chemiluminescent fluorescent and colorimetric samples depending on the system configuration and comes with analysis software The instrument can be used for research purposes in the academia and life sciences industry Amersham Imager 600 Operating Instructions 29 0645 17 AC 31 3 Instrument description 3 2 Feature overview 5 2 32 Feature overview The following features are offered by Amersham Imager 600 A highly sensitive CCD camera with 3 2 megapixels Fast detector cooling capacity Ready to use within 5 minutes after start up Tablet computer interface available Chemiluminescence imaging with high sensitivity Generation of a combined color image of a chemiluminescence sample image and a white light image of a color marker Fluorescence imaging using blue green and red Epi light sourc
91. ower ON O Power OFF Power connector Connector for attaching the AC power cord DVI D port Port for connecting a digital only DVI monitor USB port Port for additional external devices for exam ple a printer Note The tablet computer cannot be recharged with the instrument Ethernet port Connector for a network cable au 36 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 4 Instrument hardware description Amersham Imager 600 interior The illustration below shows the main hardware components inside the instrument Description Upper Tray guide Guides which support and position the tray Lower Tray guide Guides which support and position the tray Trans White light source White light Trans UV light source UV light Epi light source For configurations 600 600 UV and 600 QC White light source For configuration 600 RGB White light plus Blue Green and Red light sources Epi light source White light Filter changer Filter changer unit Amersham Imager 600 Operating Instructions 29 0645 17 AC 37 3 Instrument description 3 4 Instrument hardware description Amersham Imager 600 LED indicators The table below describes the different states of the LED indicators located at the front of the instrument to the right of the On Off button Power Run Error D 0O o POWER RUN ERROR Indication type and fre White Bl
92. p Action 1 In the Capture tab select Chemiluminescence Result The options available for chemiluminescence capturing are displayed to the right on the screen 2 If your sample contains a colorimetric marker then check the check box Colorimetric marker a Select Semi Auto exposure in the exposure drop down menu Library J Colorimetnc marker Colorimetric Aulo Semi auto Marsa Incremental Fluorescence it Exposure Semi auto L CCO status Blower position 2013 November 11 10 31 4 Select Next and wait for the pre exposure to finish 5 When the pre exposure is finished a low resolution preview image is dis played Exposure time Q Q mms Ready Lower position 2013 November 12 12 17 Use the zoom tools and arrows to get an optimal view of the area of interest Choose Q to zoom In Choose Q to zoom out Amersham Imager 600 Operating Instructions 29 0645 17 AC 95 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture 96 Step Action 6 Choose one of the two options displayed to the right of the image to point out the area of interest Choose cd then select one point of interest in the image Result The selected point is highlighted with an X Choose and then select two diagonal corners of an area of interest in the image Result The selected area is highlighted with a square Use the right hand side arrow button
93. pment PPE during operation and maintenance of Amersham Imager 600 e Protective eyewear e Protective gloves e Protective footware preferably with steel lining CAUTION Turn off the power switch before cleaning the inside of the instru ment CAUTION UV lamps in Amersham Imager configurations 600 UV 600 QC and 600 RGB contain mercury which must be recycled or disposed of in accordance with local regulations NOTICE Do not use abrasive cleaning materials such as a scouring pad for cleaning This may cause damage to the instrument NOTICE Dispose of the tablet computer as instructed in its user manual NOTICE When performing maintenance of the equipment remove the tablet computer There is risk of injury if it falls on your foot 20 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 2 Labels 2 2 Labels Introduction A label on the exterior of the Amersham Imager 600 instrument describes the specifica tions and precautions necessary to safely use the instrument This section lists and ex plains the symbols on the label and the additional symbols used on the instrument Amersham Imager 600 serial number The Amersham Imager 600 instrument serial number is found on the label located on the back of the instrument GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden LUMINESCENT IMAGE ANALYZER Model Amersham Imager 600 Serial No
94. ress the Power switch on the right side of the instrument to the O position Note To optimize the instrument s life time and to minimize unnecessary power consumption shut down the instrument when image captures and analyses have been completed for the day Ifthe Amersham Imager 600 instrument is used around the clock make sure to restart the system at least once a day to remove temporary files that may take up unnecessary memory space The saved image files are stored in the formats described in the following table Note The jpg images are created from the equivalent tif image to facilitate for presen tation purposes Amersham Imager 600 Operating Instructions 29 0645 17 AC Imaging method Chemiluminescence 5 Operation 5 7 Save and manage image files 5 7 2 Save images File format of saved image A folder containing the following files e Sample A tif image in gray scale for quantification of chemiluminescence data e A Jpg image in gray scale and with set contrast for presentation purposes Note The jpg image is included in the folder but it is not displayed on the screen Chemiluminescence with colorimetric marker A folder containing the following files e Sample A tif image in gray scale for quantification of chemiluminescence data e Marker A tif image in gray scale for quantification of colorimetric data Note Both the above files have the same pixel resolution e Sampl
95. rictly Consumables or certain parts may have their own label with an EFUP value less than the product Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures This product must not be disposed of as unsorted municipal waste and must be collected separately and handled properly after decom missioning Amersham Imager 600 Operating Instructions 29 0645 17 AC 27 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS Meaning ine RAAT mea SJ T11363 2006 HF ERT Ras BSMRNRBEK PREVARE EMA MERPHMFAA mY REA RAK REELS EAWREL 8588W RABSRERUMRE APERAS mRANM EMT ESR RIBAS UFaKSREHNRR WA 7A PRE Pir FA BB AY oR ERB DER m FAP PAN E RE AAEM HET REST PERNE RFER PE Roh A RE AE ALR E AB we RAIAR RR 3 ELLA PRUE FRAO AE LRN A SERRE RIS BIG ASE ERI EHEER ARUP AR BA AOE AF RARE FSR AP AEE ALS A RT A HE RANE ATLA RAELE List of hazardous substances and their concentrations mt ARS EMEARTRWEMREE Indication for each major part if substance exceeds limit Meaning Indicates that this toxic or hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in SJ T11363 2006 RTZASARUAEZARA IAA BSB ESI 711363 2006 PR EAN TE BY BR SE KRAT Indicates that this toxic or hazardous substance contained in at least one of the homogeneous materials used for this par
96. rk the field of view It is possible to place more than one membrane or gel provided they fit into the field of view There are two alternative tray positions with different fields of view 1 The upper tray position with a field of view of 8 x 11 cm Use the upper tray position for Chemiluminescence capturing for a smaller field of view of a single membrane and shorter exposure time 2 The lower tray position with a field of view of 16 x 22 cm Use the lower tray position for a larger field of view for one to four samples Amersham Imager 600 Operating Instructions 29 0645 17 AC 87 5 Operation 5 4 Place the tray in the instrument Step Action Note For Colorimetric Epi illumination position the gel so that the lanes are par allel to the long side of the tray as illustrated below This is important to avoid capturing artefacts in the image 4 Open the instrument door 5 Insert the tray in the correct tray position Note For Chemiluminescence capturing you may also select the upper tray position 1 in the illustration below 6 Close the door 88 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 5 Capture an image 5 Operatio n 5 5 Capture an image This section describes how to capture an image with any of the available methods In this section Section See page 5 5 1 Available methods 90 5 5 2 Chemiluminescence capture 91 5 5 3 Colorimetric capture 103 5 5 4 Fluorescence
97. rkgroup 4 Select OK to save the changes Result The connection to the network will be established within a couple of seconds 76 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 4 Network 4 4 2 Network access from instrument 4 4 2 Network access from instrument To be able to store and access files on a network location Amersham Imager 600 must have access permission to the specific computer or server where the files are to be stored The exact procedure for setting permissions for network file sharing depends on the computer s Operative System OS and your organization s network security settings Note Contact your GE representative for optional OS instructions or refer to your local IT support or your own computer s help files OS Windows 7 instructions Step Action 1 On the computer Open Control panel System and Security System and write down the name of the computer the network identification name 2 Create a new folder and name the folder Example AmershamImager600 3 Right click on the folder and select Properties Share with 4 Select which user or group you wish to grant permission for example users and check the check box Full control Note Limited permissions such as Read will not allow the instrument to export or analyze saved files on the computer 5 Select Share 6 Note the full computer name and go to the instrument 7 On the instrument Select the settings i
98. s 8 Specifications eseessessesseeseeseeseeseeseeseceseeserseeseeseesseeseeseesceseeseesseesesseeseeseeseeesessesses 103 107 117 121 122 125 130 133 136 138 141 143 146 147 149 Amersham Imager 600 Operating Instructions 29 0645 17 AC 1 Introduction 1 Introduction Purpose of this document The Operating Instructions provide you with the instructions needed to operate and maintain Amersham Imager 600 in a safe way Prerequisites In order to operate Amersham Imager 600 in the intended way the following prerequisites must be fulfilled e You have read and understood the safety instructions in this Operating Instruction e You should be acquainted with the use of general laboratory equipment and with handling of biological materials e The instrument is installed by GE representatives About this chapter This chapter contains important user information description of safety notices regula tory information and a general description of the intended use of Amersham Imager 600 In this chapter Section See page 1 1 Important user information 6 1 2 Regulatory information 8 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 1 Introduction 1 1 Important user information 1 1 Important user information Read this before operating Amersham Imager 600 All users must read the entire Operating Instructions before installing operating or maintaining Amersham Imager 600
99. s the number of pixels with a given intensity The blue and red lines indicate the min max range of the grayscale in the displayed image Use the blue and red arrows to modify the contrast Note For samples with colorimetric marker use the contrast tool for setting the contrast of the chemiluminescent sample The selected contrast is saved with the combined color im age Amersham Imager 600 Operating Instructions 29 0645 17 AC 119 5 Operation 5 6 View the image Description Illustration Open the Intensity tool by selecting the intensity button in the image tools menu Select an area of interest in the image The selected area will be displayed to the right of the image The maximum average and minimum intensities in the area of interest are displayed under the image Use the arrow buttons to make fine adjustments or point to a new area of interest in the main image Note Maximum 0 238 When multiple channels are selected for viewing only the inpri Ea Minimum 0 050 maximum value for each channel is displayed Note For incremental images the intensity tool is the only available tool Navigate between the images using the forward and backward arrows Point to a specific area of interest to dis play the maximum pixel intensisty and the average pixel intensity in the area Tip If the intensity is too low or too high consider to capture the image using a different exposure time Too high inte
100. s to move the highlighted point or area Select Start exposure and wait for the exposure to finish Amersham Imager 600 Operating Instructions 29 0645 17 AC Step Action 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture 8 When the exposure is finished the image view appears displaying the im age s Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126 No colorimet ric marker was used The resulting image is displayed in gray scale i rams ee Linear Log Colorimetric marker was used The resulting images are displayed under Sample and Marker tabs e Select the relevant tab for the image you wish to display A selected tab is highlighted with blue text and frame e Select both tabs simultaneously to see a composite color image of the Sample and Marker images 9 Select the Save button to save the images For other options after capturing see Options after capturing on page 102 Amersham Imager 600 Operating Instructions 29 0645 17 AC 97 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Chemiluminescence with manual exposure Follow the instructions below to perform chemiluminescence capture using manually set exposure time Step Action 1 98 In the Capture tab select Chemiluminescence Result The options available
101. save the analysis press Save Result The image file is updated with the analysis information 4 Choose a location to save the analysis by selecting the Where to save file drop down menu and select a location in the list 5 If desired edit the prefix of the image name by selecting the Image name prefix field Type the new prefix using the appearing keypad and select the Done button to save the changes 6 If desired add a comment by selecting the Comment field Type the com ment using the appearing keypad and select the Done button to save the changes 7 Select the Save button See Section 5 7 Save and manage image files on page 121 for details Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 9 Turn off the instrument 5 9 Turn off the instrument This section describes the instrument shutdown procedure Follow the instruction below to turn off the instrument when capturing and analysis is finished for the day Step Action 1 Press the On Off button on the front panel v Result The LED Power light shuts down 2 Press the Power switch button on the right side of the instrument to the O position Result The instrument is turned off Note To optimize the instrument s life time and to minimize unnecessary power con sumption shut down the instrument when image captures and analyses have been completed for the day If the Amersham Imager 600 instrument is used around the clock
102. soft sponge soaked in a cleaning agent After cleaning rinse the accessory immediately in water until no detergent remains and allow to air dry Cleaning gel sheets After use wash the gel sheet with mild detergent rinse with water then dry well Note A gel sheet can be reused about 20 times Amersham Imager 600 Operating Instructions 29 0645 17 AC 153 6 Maintenance 6 3 Regular inspections 6 3 Regular inspections Regularly inspect Amersham Imager 600 to ensure the following e The power supply plug is firmly secured in the power outlet e The power cord and supply plug do not become overheated e The power cord is not damaged in any way e The ventilation holes in the equipment are free from dust and dirt 154 Amersham Imager 600 Operating Instructions 29 0645 17 AC 7 Troubleshooting 7 Troubleshooting About this chapter This chapter describes various problems that may occur with Amersham Imager 600 and provides possible solutions to resolve problems In this chapter Section See page 7 1 General problems 156 7 2 Problems with image quality 158 7 3 Problems with the software 160 General troubleshooting procedure Follow this procedure if an error occurs 1 Take note of the error code and error message on the monitor 2 Turn off the instrument by pressing the On Off button on the front panel and then the Power switch on the right hand side and turn it back on again after about ten seconds
103. ssecessecesecssesestes 3 5 5 Descriptio OF Settings VIEWS anernissiiretiiireiiic n tinte C aN eE E E e A N E a NE E Metla assener aL E T a EE A AEE E A EAA EE 4 2 eramen SEUN serana a R CE a E a NO er A E EEA EE A E 4 3 1 Do eana a0 lt geen ane mera eno nee ene Panne Tee Tn nT me Tn ero 4 3 2 FOCUS pee ene Or er OT neon ter scr POM no ONO tne er re On eC aan er er ee 45 5 Talli ge gai cig at NdT O seeni 4 3 4 Se aca exerts A E cua cenertrtattan te tay pets E tentang overseas AA NENO Ge ene een TEE OORT amt eT OPE OE NT TERT E ULE aT Tete 4 4 1 CHEVY 0 S OTUD pena eee ee nn EE OT eee 4 4 2 Network access from instrument oasis octaectnnt tacctrcsisitesetenrtat sare ccincbnesaenaetess used 4 4 3 Instrument access TOI NEtWOFK cc acc a ta ch eet vetaaean unreacted dene bated I aaeee cena exter cces eos esciensis aberrant ensue pvacs on xcteeseeceararetornemcensanceeeseeeneeee Sol OPE O ON 9 EE tne ee nner eT ree ve eee ee eer ee ae TOR US A SUNN esc cece EE A so E E C41 e Senne ne ne ONE enn one A er Sen TOs 5 4 Place the tray in the instrument oou eeecceccesssssssssssecssecssecssecsssessussssscssecssecssscssecssecssessssessseesseessecssecs 55 O18 601180 9 lg 6 0 lt siise paeas EEE er OO ne aS nC On 651 PVN NUON S eiria rere iea cet esas ee iene 55 2 Chemiluminescence CAptUTe asics crs acsctcseisteascescccesslduesceriaasih tease cere ienia tues Amersham Imager 600 Operating Instructions 29 0645 17 AC 12
104. sure Auto CCD status Lower position 2013 November 11 10 56 Function Fluorescence image capturing method selected Options for the selected image capturing method e Epi RGB selected here e Custom e Trans UV Exposure options e Auto selected here e Semi Auto e Manual Note For more information on manual settings see on page 55 Amersham Imager 600 Operating Instructions 29 0645 17 AC 43 3 Instrument description 3 5 Instrument software description 3 5 2 Description of the Library tab 3 5 2 Description of the Library tab The Library tab allows you to find and manage image files The illustration below shows the main components of the Library tab Capture Library A On instrument a ed E Search Clear Sort Date V 2 1 ho131105 145157 Fl Custom 145157_Fl Custom 20131105 142553 Fl Blue 20131105_142329 Fi 20131105_141413_Co Selectall Tm 9 20131105_141023 Co one file selected _ 8 Delete p 20131105_140832 06_Ch 10 20131105_140610_Ch Rename 11 20131105_135833_Ch Copy Move mm 20131105_132642_Ch 12 20131023_131738_Fl Red v Open DN CCD status Lower position 2013 November 11 13 33 13 Function l The list of files in the selected location The selected file is highlighted in blue Note You may need to scroll or navigate between views to see all files 2 The selected file location here on the instrument Use to view file storage opti
105. t for use with Black Tray where applicable Amersham Imager 600 Operating Instructions 29 0645 17 AC 57 3 Instrument description 3 6 Accessories Description Diffuser Board marked Diffuser Board for white light Trans il lumination Code no 29 0834 20 Relevant for con figuration 600 QC 600 RGB Amersham Imager 600 Operating Instructions 29 0645 17 All configurations AC power cord for North America 19 2448 01 All configurations AC power cord for Eu rope 80 6480 33 All configurations DVD containing transla tions of Amersham Imager 600 Operating Instructions Additional accessories to order 58 Description Gel Sheets 17x25 for UV Trans tray 29 0902 97 Code no 29 0834 57 All configurations Relevant for con figuration 600 UV 600 QC 600 RGB Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 6 Accessories Upgrade options Part Description Relevant for con figuration AI600 Upgrade 600 to 600 UV 29 0834 22 AI600 Upgrade 600 UV to 600 QC 29 0834 24 AI600 Upgrade 600 QC to 600 RGB 29 0834 25 Al600 Upgrade 600 UV to 600 RGB 29 0834 26 Amersham Imager 600 Operating Instructions 29 0645 17 AC 59 4 Installation 4 Installation About this chapter This chapter contains information on how to prepare the site for Amersham Imager 600 how to set up
106. t description 3 5 Instrument software description 3 5 1 Description of the Capture tab 3 5 1 Description of the Capture tab Capture view The Capture tab is displayed as the default start screen when Amersham Imager 600 and the tablet computer are turned on and the start up procedure has finished It allows you to select image capturing method and set details for the exposure The display changes depending on the choice of method The following illustration describes the basic elements of the Capture tab Chemiluminescence 2 3 N Capture Library es 5 D 1 Colorimetric marker wit Chemiluminescence e Colorimetric Fluorescence CCD status a position 2013 November 11 ga j 9 8 7 Function 1 The selected image capturing method 2 Capture The tab for selecting image capturing method and starting ex posure 3 Area displaying options for the selected method 4 The settings icon for accessing the Settings view 5 The help icon for accessing context sensitive online help 6 Start The start exposure button 7 The date and time 8 The position of the inserted tray 40 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 1 Description of the Capture tab Part Function The instrument CCD status displaying one of e Green The CCD temperature is within specifications and the instru ment
107. t is above the limit requirement in SJ T11363 2006 e Data listed in the table represents best information available at the time of publication RRBZASA SVR ZLSEBRMEHR HRAAPHe ew SJ T11363 2006 REM E BY RSZK 28 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 5 Declaration of Hazardous Substances DoHS List of hazardous substances Component Hazardous substance name SESEMRATE BBE EN Pb Hg Cd Cr6 PBB PBDE 1 Ro m AN B RRA SRAM Amersham Imager 600 29 0834 61 1 Amersham Imager 600 UV 29 0834 63 Amersham Imager 600 QC 29 0834 64 Amersham Imager 600 RGB 29 0834 67 1 The product has not been tested as per the Chinese standard SJ T11363 2006 Requirements for Concen tration Limits for Certain Hazardous Substances in Electronic Information Product Amersham Imager 600 Operating Instructions 29 0645 17 AC 29 3 Instrument description 3 Instrument description About this chapter This chapter presents an overview of Amersham Imager 600 features and different in strument configurations It also contains descriptions of hardware components software displays and functions Note Due to cosmetic changes to the software the screen captures in this manual may differ sligtly compared to the acutal software However the general functions are the same In this chapter Section See page 3 1 Introduction 31 3 2 Feature
108. tes a folder containing the created image files which can be saved The display and number of images created depends on the number of detection channels selected in step 2 For details see File formats on page 126 gue Green Ree Three detection channels were se lected Select the relevant tab of Blue Green or Red for the image you wish to display A selected tab is highlighted with blue text and frame Select two or more tabs simultaneously to see a composite color image Two detection chan nels were selected Select the relevant tab of Blue Green or Red for the image you wish to display A selected tab is highlighted with blue text and frame Select both tabs simultaneously to see a composite color image One detection chan nel was selected Sample One image is displayed 8 Select the Save button to save the images For other options after capturing see Options after capturing on page 102 Amersham Imager 600 Operating Instructions 29 0645 17 AC 111 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture Fluorescence Epi RGB with colorimetric marker This method is used for samples containing a colorimetric marker and is only available for single channel capture Follow the instructions below to perform Fluorescence single channel capture with a colorimetric marker Step Action 1 112 In the Capture tab select Fluorescence Library e
109. the changes to date and time Select the Ok button to save the changes to the Date amp time view Select the Cancel button to leave the view without saving changes 66 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 3 System settings 4 3 2 Focus 4 3 2 Focus Focus view The focus is normally fixed For thick samples this fixed focus may not be adequate and you may need to temporarily adjust it 1 2 3 4 Focus 7 66 5 Default aie Brightness Zoom Select position on the image Cancel p i 1 7 a 9 To CCD status Lower position 2013 November 11 13 33 Function The image area which displays the image of sample Double arrow buttons Use the buttons to adjust focus in larger steps The focus value Single arrow buttons Use the buttons to adjust focus in smaller steps Default button Use the button to reset focus to the default value Note The default value differs between instruments The brightness value Plus and minus buttons Use the buttons to adjust the brightness value OK button Use the button to save the new settings Cancel button Use the button to leave the view without saving changes Amersham Imager 600 Operating Instructions 29 0645 17 AC 67 4 Installation 4 3 System settings 4 3 2 Focus Adjust focus Step Action 1 Prepare and place a sample on a tray and place the tray in the instrument s
110. the image Sample MMarker Lane Creation Number of lanes 4 TU j Lane width H Tan Move comer point ii JUL A lt gt 0 Q A v m 4 Select the minus and plus buttons adjacent to Lane width to increase or decrease the lane fill factor Note If the value is 100 then the grid contains only the lanes Using a lesser per centage creates an empty space between lanes 5 When you are done select the Next button or select the Background button in the workflow The Back button will bring you back to the captured image Amersham Imager 600 Operating Instructions 29 0645 17 AC 137 5 Operation 5 8 Analyze the image 5 8 2 Background Subtraction 5 8 2 Background Subtraction Description This is the second step of the analysis workflow The options for background subtraction are presented next to the image Tip Use the lane profile button to display separate graphical lane profiles with the current lane number displayed in the dropdown list A The illustration and tables below describe the background subtraction options Note The selected method subtracts background for all lanes in the image Option Description None Use this option when no background should be subtracted from the image Rubber band Use this option to subtract the background below a base line between the lowest points on the lane profile as though a rubber band was stretched under the profile
111. tion 3 5 Instrument software description 3 5 3 Description of the image view Part Function The image tools menu Use the tools to modify the settings of the captured image and to view details y Display Contrast Intensity tool For more information on the image tools see Image tools on page 118 The selected image tool and options view Display is the default view Done button Use the button to leave the view without saving Note A dialog appears asking to confirm the cancellation This prevents deleting images by accident Save button Use the button to save the image See Section 5 7 Save and manage image files on page 121 for further details Analyze button Use the button to open Analyze view See Section 5 8 Ana lyze the image on page 133 for further details Print button Use the button to print the image Note The print function requires that the instrument is connected to a USB con nected printer For installation of a printer contact your GE representative 48 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 4 Description of the analysis workflow 3 5 4 Description of the analysis workflow This section describes the analysis workflow options The workflow allows you to perform an analysis of the image data Open the analysis workflow by selecting the Analyze button The button is available after capturing
112. to avoid personal injury or damage to the instrument General precautions WARNING Do not operate Amersham Imager 600 in any other way than de scribed in the User Manual WARNING Use of this equipment in other ways than those specified in the user documentation may result in physical damage because of exposure to irradiated light or electric shock or injury from touching an operating part WARNING Do not use the instrument if smoke atypical noises or odors can be perceived or if the instrument becomes unusually hot as this may result in fire or electric shock Stop using the instrument immediately turn off the power switch and unplug the instrument from the power outlet Contact your local GE representative to request repair Amersham Imager 600 Operating Instructions 29 0645 17 AC 13 2 Safety instructions 2 1 Safety precautions WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock If the power supply cords are damaged contact your local GE representative for replacements WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect WARNING Do not blo
113. tration OSHA has recognized as meeting the legal requirements in USA title 29 of the Code of Federal Regulations 29 CFR Part 1910 7 22 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 2 Labels Position of label and additional symbols This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numerique de la classe A est conforme a la norme NMB 003 du Canada Note For details on symbols see Section 3 4 Instrument hardware description on page 54 Symbol Description On Off switch Amersham Imager 600 Operating Instructions 29 0645 17 AC 23 2 Safety instructions 2 2 Labels Description Power switch Power ON O Power OFF Connector for DVI D monitor USB port Ethernet port Caution hot surface 24 Amersham Imager 600 Operating Instructions 29 0645 17 AC 2 Safety instructions 2 3 Emergency procedure 2 5 Emergency procedure In case of emergency e Turn off the power by pushing the Power switch to the O position on the bottom right hand side of the instrument e Disconnect the power cord from the wall socket WARNING Access to
114. uelight Redlight quency light Power up Flashing 1 Hz ON 0 5s OFF 0 55 Ready Power Steady light on Busy Run Steady light Flashing 1 Hz ON 0 5s OFF 0 5s Shutdown Flashing 1 Hz ON 0 5s OFF 0 5s Power save Flashing slowly 0 5 Hz ON 0 5s OFF 1 5s 38 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 Instrument description 3 5 Instrument software description 3 5 Instrument software description Introduction The Amersham Imager 600 software is designed to guide you through the workflow of capturing images and analyzing the image data The software is normally operated on a wireless connected tablet computer placed in the dock on the instrument front This section describes the main components and functions of the software Note The software can be controlled from a handheld tablet computer a wire connected touch screen or a monitor plus mouse Note The available functions may vary depending on the system configuration For details on system configurations see Section 3 4 Instrument hardware descrip tion on page 34 In this section This section contains the following Section See page 3 5 1 Description of the Capture tab 40 3 5 2 Description of the Library tab 44 3 5 3 Description of the image view 46 3 5 4 Description of the analysis workflow 49 3 5 5 Description of settings views 53 Amersham Imager 600 Operating Instructions 29 0645 17 AC 39 3 Instrumen
115. ument in a place exposed to direct sunlight Operation site Indoor use Maximum operating altitude 2000 m or lower Noise 70 dB A or lower Fast Lmax 60dBI A and average Leq lt 54dB A 1 m from the instru ment Protection provided by enclosure IP21 Overvoltage category Transient overvoltage category Il Rated pollution applied 62 Pollution degree 2 Amersham Imager 600 Operating Instructions 29 0645 17 AC 4 Installation 4 2 Instrument setup 4 2 Instrument setup Introduction Amersham Imager 600 is initially installed by GE service representatives Amersham Imager 600 must be secured before long distance transports Contact GE for instructions before transport or relocation of the the instrument For connecting additional monitors or peripheral equipment contact GE for instructions Amersham Imager 600 Operating Instructions 29 0645 17 AC 63 4 Installation 4 3 System settings 4 5 System settings Introduction 64 This section describes how to set system specific settings System settings are found under the Settings view Open the settings view by selecting the settings icon found in the top right hand corner of the Capture and Library tabs Capture Library Chemiluminescence Colorimetric marker ui Chemiluminescence Colorimetric 4 Fluorescence Exposure ccDstatus Ss Lower position _ 2013 November 11 13 33 For the general compo
116. ut saving changes 2 Reset button Use to reset the the exposure time to zero Amersham Imager 600 Operating Instructions 29 0645 17 AC 55 3 Instrument description 3 5 Instrument software description 3 5 5 Description of settings views Function Time units area Use the plus or minus buttons to increase or decrease the exposure time by Minutes Seconds or 1 10 sec Keypad Use the right and left arrow buttons to navigate between the Minutes Seconds and 1 10 sec fields The active field appears blue in the time units area Use the numeric keys to set the time in the selected field Arrow buttons Use the buttons to navigate between minutes seconds and 1 10 s in the time units area Set button Use the button to save the changes made 56 Amersham Imager 600 Operating Instructions 29 0645 17 AC 3 6 Accessories Accessories delivered with the instrument 3 Instrument description 3 6 Accessories The following table shows the accessories of Amersham Imager 600 Depending on configuration the included accessories may vary Part Description Code no Relevant for con figuration F Black tray marked 29 0834 17 All configurations Black Tray UV Trans tray marked 29 0834 19 600 UV UV Trans Tray 600 QC transparent 600 RGB White Trans tray 29 0834 18 600 QC marked White Trans 600 RGB Tray White Insert marked 29 0880 60 All configurations White Inser
117. when the pixel intensity of the accumulated exposed images reaches satu ration Note The Stop when saturate function overrides the set repetitive exposures Set Interval time by pressing the value Result This opens the Set interval time view Listary Lei Set imbere tim You can upe byth the numeric kgya dred the i buibor ip sai time Mimes Seconds g Ready Lower postion 2013 Nowember 10 13 53 Amersham Imager 600 Operating Instructions 29 0645 17 AC 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture Step Action 6 Enter the value and select Set to to save the value and to return to the pre vious view The Reset button resets the value to zero 7 Select the Start button and images are captured repeatedly for the set time period or until saturate Result The number of images matching the selected repetitions are displayed as they are exposed A progress bar indicates the time remaining of the imaging process 1 10s Stop Exposure in coes EE CCO status A Lower position 2013 November 11 10 51 8 When the exposure is finished the image view appears displaying the im age s You can navigate among the images by selecting the left or right ar rows You can also use the intensity tool to check pixel intensity Amersham Imager 600 creates a folder containing the created image files which can be saved For details see File formats on page 126

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