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F-900 Operation Manual

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1. 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 40 sales Qfelixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 6 Connect the in tubing to the intake port on the instrument 7 To use the buffer bottle make sure that the tubing is clear from obstruction Connect the In tubing to the unit Place the buffer bottle in a location that has stable CO2 concentration away from operator cars furnace photosynthesizing plants Here ambient air CO gt levels should be approximately 400 ppm Note The instrument shown in these images is a Cl 340 Photosynthesis Analyzer The buffer tank system technique can be used to help stabilize the ambient intake of any IRGA CO sensor images courtesy of ZealQuest China 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 41 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 O Calibration The offset and gain from the previous calibration can be seen under Calibration Parameters These values are achieved by setting a zero and a span for the unit To re calibrate pressurized N gt gas and O2 gas will be needed If O2 gas is not available ambient air may be used Annual re calibration is recommended but if the environment changes frequently re calibration may be required more often To set the zero of the O2 sensor follow the same instructions fo
2. INSTRUMENTS APPHED FOOD SCIENGE F 900 Portable Ethylene Analyzer 900 Portable Ethylene Analyzer DOCUMENT OVERVIEW Mod 1 EE EE EN Ea Taa ae 2 SDS Il CAI ea E E E E EEA R 3 TEN PNG 5 aS ge KE EEE EET yaeamemesens nos sae 6 Kane 7 PT 8 PAN 8 MATEN 9 FORS NNN E 10 EIERE EE EE EE EE EEE EN NE EE NE JE EN 11 CATENE Ae eg EE EE EE 12 F 900 CO2 PPM Sensor and Battery Power 13 Measure Menu Taking a Measurement rrrnnonnrnnnnnnrnnnnnrrnnnnnrnnnnnsrnnnnnsennnnernnnnnsrnnnnesnnnnnernnnnssrnnnnesennnnssnnnnssennnnnsennn 14 EE DASE TE EEA EN RE T 14 Ge El Be e 15 Monitor ee TEE 16 F 900RK Using the Research Kit Fruit Chamber 20 NNN MOO O oien E E E E E E A 22 Ethylene Sensor Zero CaliDration siiceciissccinssiccsovassnassverssasenecdsvedenebadsaveidonssiusuadessuesivodusrsedeessvdeenddaveiseawdasvesdansasdarsaneas 28 Mr 28 Automatically Set Zero Offset Autocorrechlon 28 Stil Ie UN EE VE EE EE NR EE EE NE EE EE 29 Sop leegent egene ee e Seege EE 29 ANN 29 ELT Se LEE 31 e140 Cl de EE 34 e E ANNO 0 EEE EEE EE E 34 ENN 35 NN NNN 35 ON 36 NNN 42 mio sm Die KR 43 SJ OE NE asses aeapeai aneaedeneapeaneneene 43 MTU 44 NTN 48 Se NII gt or EE 50 PENN 50 VANN 51 VG 51 MOV e 52 VNR 52 Other View Menu Options EEE 53 GPS System E E 53 E EE 54 EEE 54 DTS 55 EE 55 MN 57 Data Maier E ge EE EE a 58 Deer 59 Gun VS Reie E EEE Se cme ee E 60 Cleaning NNN 64 Maintaining the Electrochemical S
3. Dispersion Properties See solubility in water Solubility Easily soluble in cold water hot water Insoluble in methanol diethyl ether n octanol SECTION 10 STABILITY AND REACTIVITY DATA Stability The product is stable Instability Temperature Not available Conditions of Instability Not available Incompatibility with various substances Extremely reactive or incompatible with alkalis Slightly reactive to reactive with metals Corrosivity Extremely corrosive in presence of aluminum of zinc Highly corrosive in presence of steel of copper Slightly corrosive to corrosive in presence of stainless steel 304 of stainless steel 316 Non corrosive in presence of glass Special Remarks on Reactivity Reacts violently with water especially when water is added to the product Sulfuric acid Special Remarks on Corrosivity Not available Polymerization No SECTION 11 TOXICOLOGICAL INFORMATION Routes of Entry Eye contact Inhalation Ingestion Toxicity to Animals LD50 Not available LC50 Not available Chronic Effects on Humans The substance is toxic to lungs mucous membranes Other Toxic Effects on Humans Extremely hazardous in case of skin contact corrosive irritant of ingestion of inhalation Special Remarks on Toxicity to Animals Not available Special Remarks on Chronic Effects on Humans Not available Special Remarks on other Toxic Effects on Humans Not available 1554 NE 3 Ave Camas WA 98607
4. please visit the F 900 software webpage http felixinstruments com support f 900 support The line underneath this states Felix Instruments Below this a menu will appear on the display with the following options Measure Setup View and File You are now at the F 900 Main Menu After the instrument is powered on the uptime and battery life remaining will be displayed on the bottom line The uptime or time the instrument has been powered on is displayed in hours minutes followed by the battery life in percent Note If the F 900 does not power on eject the SDcard or disconnect the mini USB cable from the front of the instrument Then power the F 900 on Re insert the SDcard or reconnect the charger INSTRUMENTS F 000 v4 4 0 108 Felix Instruments F 900 Portable Ethylene Analyzer Figure 8 The options on the Main Menu of the F 900 From the Main Menu there are four menu options Measure Setup View and File Use the UP and DOWN arrows to switch between menu options and the RIGHT arrow to select Press the POWER button to power off the F 900 Note To force a power off and reset of the F 900 at any point a user may press and hold the POWER button for 10 seconds This emergency hard shut down feature exists to address hardware or software problems and should not be used except when needed in general simply pressing the Power button in the typical manner will power off the F 900 1554 NE 3 Ave Camas WA 9
5. Optional Sensors Included Yes No C02 IRGA ppm CO2 ER 3 02 Gi se C2H4 STD MG NOTES 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 85 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 86 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 201 Warranty Registration Card INSTRUMENTS APPLIED FOOD SCIENCE 1554 NE 34 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 e mail sales felixinstruments com Web www felixinstruments com PRODUCT REGISTRATION CARD Please complete and return this form to Felix Instruments within 30 days to validate your Warranty on Parts and Labor Registration Information Your Name Title Company University Address City State Zip Country Email Phone Fax Felix Instruments Serial Number s Purchase Date Purchase Price FOLD ON DOTTED LINE Your opinions will help improve our service Please answer the following questions What was the basis of your product selection Representative Recommendation o Price Product Features o Product Design o Technical Specifications o Brand Name o Warranty o Service o Other O N What other competing brands did you consider 3 Where did yo
6. Temperature Ar Optional PD c02 02 a After 15 minutes the ethylene concentration of the gas in the closed loop should be close to or equal 0 ppm Choose the Set Zeros to correct the offset baseline shift of the C2H4 sensors by pressing Enter twice when C2H4 is highlighted Ethylene C2H4 F Outlet Automatically Set Zero Offset Autocorrection Please see Setup gt Calibration gt Offset Autocorrection on page 34 for additional information or to enable disable the automatic set zero feature The automatic offset will stop a measurement to set the zero and then re start the measurement automatically This should be used during continuous monitoring or experiments longer than 24 hours The offset autocorrection procedure typically takes about 20 minutes and takes place during Sensor Stability the first screen when measurement mode is initiated The default interval between offset autocorrections is 24 hours for continuous use or during the next start up for units that are not running continuously If the unit is exposed to concentrations greater than 200 ppm during the measurement period the offset autocorrection will be initiated the next time the user enters Sensor Stability even if less than 24 hours have passed 1554 NE 3 Ave Camas WA 98607 USA sales felixinstruments com Phone 360 833 8835 Fax 360 333 1914 http www felixinstruments com 28 5 F 900 Instruction Manual rev 4 2 2015 Setu
7. The F 900 includes the consumable potassium permanganate which is used to scrub ethylene and other hydrocarbons from the gas stream If the instrument has an optional CO2 sensor soda lime is included which is used to scrub carbon dioxide from the gas stream and to create a 0 ppm CU gas for the CO zero calibration F 900 Research Kit Accessories The F 900 Research Kit F 900RK includes parts to non destructively sample fruit in the field The F 900RK arrives in a hard sided carrying case with a fruit chamber and wand with flexible tubing to sample fruit or plants The fruit chamber includes a closed chamber lid and a lid with a seal to allow sampling of fruit still intact on the plant The F 900RK includes all the parts and accessories that arrive with the F 900 as well as an external conditioning tube sample bag injection port assembly glass syringe needle and a jar of silica gel which can be used to dehumidify the gas Figure 7 The F 900RK arrives with a fruit chamber and two lids closed chamber lid left and slit chamber lid right 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 10 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Operating Instructions To turn the instrument on press the green power button The top of the display reads F 900 and the current firmware version the unit is running For information on the latest firmware version
8. g f 4 i een ee RH In Temperature Ar Optional N c02 02 SETTINGS Chamber OUT On Chamber IN Off Closed Loop On Set zeros C2H4 e Ethylene C2H4 Uptime 01 15 Bat 75 Figure 11 Measurement Settings menu display left flow path of internal gas stream right Electrochemical Sensor Response The lag in sensor response is a natural phenomenon for charged porous membrane electrodes The platinum electrode can be thought of as a series of cylindrical pores Each of these pores will have a double layer in the radial direction The interactions between the molecules convection from air movement electric fields and the concentration gradient result in a lag following a change in concentration Additionally a lag is created due to the volume of internal tubing the instrument contains Because of the volume of internal tubing it takes about 120 seconds to see a response by the sensor Auto Escape Feature An upper limit auto escape feature acts as a safety feature of the unit This safety feature is always on If the sensor detects over 200 ppm ethylene the F 900 will auto escape from monitor mode to 1 Kontturi et al Ionic Transport Processes In Electrochemistry and Membrane Science Oxford University Pess 2008 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 18 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 prevent poisonin
9. value format to be opened with Microsoft Excel or other spreadsheet program 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 29 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 In the Setup Autosave menu the top line of the display will read enter and save and the directional arrows Next to is Autosave and Yes or No indicating whether this feature is turned on or off Below Autosave is the Interval option where the user can set the time interval This is the length of time in seconds between saves when the autosave feature is selected The default autosave interval is 10 seconds Autosave Autosave Interval To set the instrument up to automatically store measurements press the RIGHT arrow key to highlight the word no on the display then press the UP DOWN arrow to switch to yes Press the SAVE key to save the configuration After saving it will exit to the Setup Menu To set the instrument up to not automatically save measurements toggle the UP DOWN arrow key until no is on the display then press the SAVE key to save the configuration When Autosave is highlighted press the UP DOWN arrow to get to the Interval line and then use the RIGHT arrow key to highlight the time interval value Press the UP DOWN arrow key to change the time interval Press SAVE to save changes To avoid chan
10. 2 2015 The F 900 will perform an action at the high and low levels when set Ifthe concentration of ethylene rises above the Gas Hi the gas will be shut off If the ethylene concentration is lower than the Gas_Lo the gas will be turned on Similarly the fan control is used to turn on and off a fan based on ethylene concentration Ifthe ethylene concentration rises above the Fan_Hi level the F 900 will turn on the fan in order to evacuate a room or chamber and reduce the ethylene concentration The Fan Lo setting is to turn off the fan The Gas and Fan controls can be used together but be sure that the Fan_Lo setting is well above the Gas_Hi setting To connect a fan or gas control to the terminal block of the F 900 first pull off the removable portion of the terminal block pull straight out The piece in the figure below will separate from the F 900 Slide the wire from what is going to be controlled through the opening for pin 4 or pin 5 Use a screw driver to tighten and clamp onto the wire creating a connection The removable section of the terminal block is designed to stay with the fan or gas control So if the F 900 needs to be moved the terminal block can be disconnected easily 12 3 45 6 7 8 910 Figure 17 The removable piece of the F 900 terminal block The 10 pins of the terminal block have the following designations PIN Function 6 Alarmorerrorindicator gt 1554 NE 3 Ave Camas WA 98607 USA Phone 360 83
11. 8835 Fax 360 833 1914 22 sales Qfelixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 How GC Emulation Mode Works The patent pending Flow Injection Analysis method integrates the signal from the ethylene sensor for 90 seconds The resulting value reported as area counts is then compared against a user definable calibration point and an estimated concentration is determined Using GC emulation mode requires the use of a standard ethylene gas The user definable calibration point should be determined on a technician by technician basis as injection technique will alter the result Additionally setting the calibration point near the expected ethylene concentration will provide the most accurate estimation There are several improvements compared to a Gas Chromatograph No need for N2 Hz or bottled air Portable No heating or ovens required Predicts ethylene concentration without chromatography gt gt GC Emulation Mode Tips Ifinjection port does not have pressure release valves please disconnect and reconnect port at end of each measurement when result displayed to prevent pressure built up inside unit Ifthe expected C2H4 sample s concentration is gt 2ppm use a higher flow rate to increase signal detection Recommended flow rate is 250mL minute and can be set in Setup gt Flow menu In general higher injection volume will also increase signal detection Recommended
12. 900 a The F 900 and F 900RK ship with potassium permanganate KMnO04 an ethylene scrubber Purafil Fresh Air Sachets KMnO4 is sold at many Home and Garden stores and online The pellet vs crystal or powder form of KMnO is recommended i http www producefreshies com li http www water softeners filters com potassium permanganate free flowing 2 lbs php gclid CjwKEAjwqamhBRDeyKKuuYztxwQSJAA1luvG990d7hn1 UO9syxQjxnCTkdfFe22M bttL t8qgs7QhoC2Kfw wcB 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 69 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 b The F 900RK also ships with a humidity scrubber silica gel If the F 900 includes an optional C0 gt 2 sensor soda lime is provided as a CO2 scrubber Carolina Biological www carolina com is an online source for soda lime and Silica gel 7 What are some possible applications for the F 900 a The F 900 could be used to help optimize Controlled Atmosphere Storage Rooms and prevent losses Ethylene is an important and sensitive marker for ripening of fruits Other postharvest research applications are possible b In addition to plants some microorganisms including fungi and bacteria synthesize ethylene Microorganisms can cause great losses in the postharvest industry through disease and mold so research into ethylene and the pathogen host interaction is important A common plant pathog
13. An example of climacteric fruit is bananas they are picked and shipped green and then ripen ata later time often in the store or home Climacteric fruits include but are not limited to apples apricots avocados bananas cantaloupes figs guavas kiwis mangoes nectarines peaches pears persimmons plums and tomatoes Source Wikepedia com 2 What should I do if I see moisture develop in the tubing when monitoring a fruit in the chamber a There is a protective hydrophobic filter inside the IN port on the front of the F 900 This will prevent moisture from fully entering the instrument Silica gel in a conditioning chamber can be used to dry out the gas stream either before or after the gas passes the electrochemical sensor Refer to the flow path diagram in the Setup gt Chamber section of the User Manual for more information 3 Canthe data output be directly linked to the computer and by what type of connection can be used And is it MAC and or PC compatible a The unit has a SD card and the format can be read on any Mac or PC Data can also be downloaded via USB See the Setup gt Connection section on page 50 for more information on linking the F 900 to a computer 4 How do I change how the data appears in the csv file a If data isn t displaying properly try saving the file as a csv file type and re opening it If saving as a csv does not fix how the data is displayed you may need to change the separator value on
14. Out and it should be turned on Possible reasons to condition the air returning to the fruit chamber include scrubbing humidity with silica gel or scrubbing CO gt with soda lime Build up of carbon dioxide may inhibit fruit respiration at high concentrations If the sensor fails to consume all the ethylene KMnO should be used to scrub ethylene Figure 12 Using conditioned air to replenish the fruit chamber by connecting the IN and the OUT left using ambient air to replenish the fruit chamber by disconnecting the OUT right 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 20 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 To sample a fruit Open a data file to save the measurements to Attach the chamber to the F 900 by screwing on the wand to the top of the chamber Next connect one of the tubes from the wand to the IN port on the front of the F 900 Connect the black power chamber cable to the left of the display to power the fans in the fruit chamber Place the fruit inside the chamber and close it Observe and record the rate of change of ethylene over time with the F 900 Monitor Mode 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 21 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 GC Emulation Mode To use the F 900 to analyze a small s
15. Phone 360 833 8835 Fax 360 833 1914 5 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Chamber In Chamber Out Solids gt eee EEE EEE e Closed Loop pl O Intake Outlet Figure 4 Flow path diagram of the F 900 ethylene analyzer Dashed line indicates optional flow path capabilities Interfering Gases No analytical method is completely specific Gases present in the environment other than the target gas of ameasurement may affect instrument response Interferences are not necessarily linear and may also exhibit time dependent characteristics The following table details the approximate concentration in parts per million of interfering gas required to cause a 1 ppm detection in the ethylene analyzer Please note that the response values given are not absolute 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 6 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Table 1 Interfering Gas and the F 900 sensor INTERFERING GAS acetone acetylene CO CO CO ethyl ethylene gi H isopropyl N O NH alchohol glycol d alcohol Negative interference Ripening fruit emit a complex mixture of hydrocarbons including ethylene Oxidation of these other gases in the electrochemical sensor cannot be readily distinguished from ethylene This causes the ethylen
16. USA Phone 360 833 8835 Fax 360 833 1914 79 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 SECTION 12 ECOLOGICAL INFORMATION Ecotoxicity Not available BOD5 and COD Not available Products of Biodegradation Possibly hazardous short term degradation products are not likely However long term degradation products may arise Toxicity of the Products of Biodegradation The products of degradation are more toxic Special Remarks on the Products of Biodegradation Not available SECTION 13 DISPOSAL CONSIDERATIONS Waste Disposal SECTION 14 TRANSPORT INFORMATION DOT Classification CLASS 8 Corrosive liquid Identification Sulfuric acid solution UN2796 PG II Special Provisions for Transport Not available SECTION 15 OTHER REGULATORY INFORMATION Federal and State Regulations TSCA 8 b inventory Sulfuric acid Water Other Regulations OSHA Hazardous by definition of Hazard Communication Standard 29 CFR 1910 1200 Other Classifications WHMIS Canada CLASS D 2A Material causing other toxic effects VERY TOXIC CLASS E Corrosive liquid DSCL EEC R35 Causes severe burns HMIS U S A Health Hazard 1 Fire Hazard 0 Reactivity 0 Personal Protection National Fire Protection Association U S A Health 1 Flammability 0 Reactivity 0 Specific hazard Protective Equipment Gloves Full suit Vapor respirator Be sure to use an appro
17. Yes std offset 1919652 ER1 offset 0054782 std gain 00302 4 interval hour 024 duration min 030 requestion Yes Sensitivity Correction The sensitivity correction is for adjusting the internal gain value of the C2H4 sensors Ethylene gas standards are required as well as installation and use of separate software program The Sensitivity Correction feature is currently in process will be completed in a future version of firmware This will replace the manual internal gain adjustment 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 35 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 CO Calibration To access the Calibrate CO2 feature of the F 900 press the RIGHT arrow key when Calibration is highlighted on the Setup Menu Press enter to continue to the Calibration mode Select the CO2 sensor to be calibrated PPM or PCT CO2 CALIBRATION CO2 PPM CO2 PCT The CO2 sensor needs to be enabled in the SETUP gt SENSOR menu to access the SETUP gt CO2 menu To calibrate the CO2 PPM sensor 400 ppm or 600 ppm standard C0 gt gas is recommended To Calibrate the COz PCT 4 20 C0 gt is recommended Calibration Parameters will display the coefficients from the previous calibration Re calibration allows the user to re calibrate the sensor setting a new zero and span for the selected sensor CO2 PPM zero calibration is recommended weekly CO2 PCT zero
18. and what the area being used for the peak counts can be found here However it is recommended to run the standard calibration injection protocol above and have the F 900 automatically provide the peak counts 1 Take 3 measurements of a calibration gas standard 2 Determine the average of the 3 peak counts 3 Set the value for peak counts to the average and enter the ppb concentration in the calibration menu Setup gt Calibration gt press Enter to continue gt C2H4 gt Standard gt Injection parameters Injection parameters peak counts 1103698 concentration 0001000 calibrated Volume 50 0 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 27 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 201 Ethylene Sensor Zero Calibration The baseline or zero of the ethylene sensor is known to drift The zero calibration should be performed daily This can be done manually or automatically as described below In both cases potassium permanganate KMnO4 is used to scrub ethylene from the gas and set the zero Manually Set Zero Chamber In Water A Please see Measurement gt Settings menu on Chamber Out Solids page 17 for additional information 1 Place potassium permanganate KMnO3 in Chamber Out 2 Enable Chamber Out On and enable Closed loop On 3 Let the F 900 run with KMn0O in the closed loop for 10 15 minutes RH In
19. calibration is recommended monthly However if the unit is in an environment where the temperature fluctuates frequently zero calibration may be necessary more often than the recommended intervals The Measure gt Settings menu can be used to carry out the zero calibration on the CO2 sensors Connect the 0 ppm CO2 gas to the intake of the instrument Allow the gas to run for 20 30 minutes before accessing the Measure gt Settings menu Press the down arrow to get to set zeros Press the right arrow to get to the next column and use the up down arrows to find CO2 When CO2 is highlighted press ENTER twice to set the zero SETTINGS Chamber OUT Off Chamber IN Off Closed Loop Off Set zeros CO2 Uptime 01 15 Bat 75 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 36 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Alternatively a CO2 scrubber such as soda lime can be placed in Chamber OUT In this case use the following settings SETTINGS Chamber OUT On Chamber IN Off Closed Loop On Set zeros C02 Uptime 01 15 Bat 75 Turn the ethylene C2H4 sensors on for the CO calibration Directions for using a buffer system in place of a standard gas may be found at the end of the section Figure 14 Calibrating the CO2 sensor using standardized gas The CO2 PPM IRGA zero calibration will drift with time and temperature therefore weekly
20. calibration is recommended when using the CO2 PPM sensor If performing the CO2 span calibration it should be carried out immediately after the CO2 zero calibration The CO2 span or gain calibration does NOT need to be performed every time the C02 zero is set When performing the CO2 span calibration use standardized gas with at least 100 ppm COz but not over the range of the CO2 sensor 2000 ppm or 3000 ppm check the production test check sheet at 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 37 sales Qfelixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 the end of the manual for CO2 sensor information The recommended standard gas concentration for the high resolution CO sensor is between 400 and 600 ppm The recommended concentration for the low resolution CO2 sensor is 4 20 CO2 Calibrating the CO2 sensor with standardized gas is fairly simple and takes 20 30 minutes Remember to always use a T connection to vent excess gas and prevent damage to the internal components 1 Goto Setup gt Calibration press enter then select CO2 Select the CO2 sensor to calibrate 2 Go to Re calibration and press the right arrow You will hear the valves open and the pump begin to run 3 The display will read Setup N2 and press enter If you have pure N2 gas 0 ppm COz connect it to the inlet of the device Be sure to have a T in the line so that the instrument a
21. felixinstruments com F 900 Instruction Manual rev 4 2 2015 Calibration Results Zero 0 span 600 0000 If access to standardized CO gas is limited ambient air can be used to calibrate the C0 gt 2 span Ambient air should only be used if a buffer tank system is implemented The buffer tank system will help stabilize fluctuations in CO gt levels providing an ambient air with approximately 400 ppm CO depending on proximity to urbanized areas Tips for Calibrating the F 900 CO2 Sensor with a buffer system The operator should stand away from the F 900 Use extra tubing to get the intake source away from human breath Use a buffer tank system to stabilize the intake source CO Span Buffer Tank System Instructions 1 Find and clean an empty bottle with cap which is at least 3L in volume 2 Drill two small holes in the cap of the buffer bottle 1 hole is for the intake tube and 1 hole is for the out tubing 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 39 sales Qfelixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 3 Inserta short plastic tube into the cap of the buffer bottle for the out 4 Insert a longer plastic tube into the cap of the buffer bottle that will connect to the IN of the instrument Use hot glue or otherwise Seal the tubing to the cap with no leaks 5 Make sure that the tube and cap has no leaks
22. http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 If this message is seen make sure the F 900 is connected via mini USB the driver is installed the F 900 is powered on After the firmware update is successful it is recommended to let the F 900 measure with the Closed Loop ON overnight for 12 hours before using again 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 63 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Cleaning and Maintenance The F 900 housing can be wiped down with a damp cloth if the unit becomes dirty or dusty Please make sure to always keep the IN and OUT ports on the front panel clear from any obstructions The water in the electrochemical cell of the unit needs to be replenished periodically using distilled water The exact hours of use before this will depend on the humidity and temperature of the environment the F 900 is typically used in Warm or low humidity dry environments will required more frequent refills Please contact support Qfelixinstruments com for more information or to schedule instrument servicing Maintaining the Electrochemical Sensor The liquid reservoir must contain a sufficient amount of electrolyte at all times The electrocatalytic sensor operates utilizing water and therefore requires replenishment of the electrolyte solution 1 ml day while operating using continuous mode
23. injection volume is 10 15ml Once injection volume is set please make sure to maintain the same volume of injection throughout all measurements The F 900 requires some warm up time when using GC emulation mode It is recommended to verify your calibration point after it is set for the first time Inject the same sample and compare the displayed result with the calibration concentration Reset the calibration point if needed For example if the calibration point is 5 0 ppm and your verifying sample read 5 9 ppm reset the calibration point by pressing Enter Repeat this until samples are reported consistently C2H4 5 9ppm Set as Calibration Yes Enter No Stop Note Injection mode standard error at two sigma is 10 of calibration concentration 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 23 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 GC Emulation Mode Operation Procedure Section 1 Warm up 1 Turn on GC Emulation mode in the Setup gt Measure menu Press Save to save changes and exit to the main menu Measure GC Emulation 2 Enter the measure menu and wait for the instrument to stabilize SENSOR STABILITY File file01 Flow 200 mL Initialization Automatic Environment Stabilizing Please Wait 42 Uptime 00 11 Bat 98 3 Follow the instructions on the display and connect the inline injection port Press Enter S
24. pressing the RIGHT arrow key when Measure is highlighted on the Main Menu display Make sure the IN and OUT ports on the front panel of the instrument are clear from any obstructions Create and open a file before starting a measurement otherwise the default filename on the SDcard is File01 If sampling ambient air turn the unit on and leave the front ports free of obstruction If sampling a container or pallet connect the black tubing to the In port and place the end of the tubing into the container or pallet to be sampled If using the F 900RK place the fruit to be sampled in the fruit chamber and connect it to the IN port on the unit It is up to the specific setup whether the OUT tubing is connected to the OUT port with Chamber Out filled with potassium permanganate KMnO04 and set to on If left disconnected from the OUT port the fruit chamber is allowed to replenish with ambient air Sensor Stability The F 900 will display the Sensor Stability screen after pressing the RIGHT arrow to get into the Measure Menu The F 900 will begin to warm up The display will show the File that is open and the Flow of gas being pumped through the instrument Below this will be whether the initialization is automatic or manual If the initialization is automatic the F 900 will precede directly to the Graph View and Monitor View once the sensors have stabilized If the initialization is manual Monitor View will not be triggered until the u
25. should increase when you connect the USB charger V_3volt is the digital voltage from the CPU and the V_Analog is the converted analog voltage View Voltages V_System 5 98v V_Charger 4 64v V_3volt 3 31v V_Analog 2 07v View Battery The View Battery menu displays indicates on the top line if the unit s Charging is On or Off or if the unit is connected via USB cable to acomputer Press the UP DOWN arrow to change the display and see the more information about the battery The QBAT value shows the battery level in hexadecimal follow immediately by the battery level in percent VBAT shows the voltage of the battery and TBAT indicates if the temperature of the battery is ok The RO shows the output status of the battery charger Press the LEFT arrow or STOP to exit to the View Menu Off a413 60 3 840V OK Off v6 When the instrument is connected to the charger the top line will indicated Charge On Charge Off indicates that the unit is not charging USB Host in the upper left corner indicates that the unit is connected to the computer or that the power supplied by the charger is not full charging voltage unplug any other devices using the same charger 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 52 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 The QBAT hexadecimal number should be changing and the battery percent increasing
26. the repair or replacement of any part of the instrument without charge if Felix Instruments examination discloses that part to have been defective in material or workmanship and in no event shall exceed the furnishing of replacement parts F 0 B the factory where originally manufactured No equipment may be repaired or altered by anyone not authorized by Felix Instruments Material and equipment covered hereby which is not manufactured by Seller is to be covered only by the warranty of its manufacturer Seller shall not be liable to the Buyer for loss damage or injury to persons including death or to property or things whatsoever including but without limitation products processed by the use of the equipment or for damages of any kind or nature including but without limitation loss of anticipated profits occasioned by or arising out of installation operation use misuse nonuse repair or replacement of said material and equipment or out of the use of any method or process for which the same may be employed The purchaser is to pack ship or deliver the instrument to Felix Instruments in Camas Washington USA within 30 days after Felix Instruments has received written notice of the defect at the customer s expense No other arrangements may be made unless otherwise approved in writing by Felix Instruments The use of this equipment constitutes Buyer s acceptance of the terms set forth in this warranty There are no understand
27. to adjust the internal gain reading for the CH PPB sensor To adjust the internal gain of the GH PPM sensor standard gas between 5 100 ppm could be used The timeframe for adjusting the gain depends on the specific sensor and its use and could be needed as frequently as every 2 months Run the calibration gas for at least 15 minutes to ensure correctly reading calibration standard Use the following formula to find the new internal gain and update it in the Calibration Parameters menu where solving for x is the new internal gain The measured concentration is what the F 900 is reading the calibration gas as Enter the temperature on the display of the F 900 as the calibrated T when the gain is adjusted A measured concentration Original gain standard gas concentration 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 34 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Offset Autocorrection Offset autocorrection allows the unit to adjust the baseline or zero for the ethylene sensors which may drift from the zero set at the time of calibration The most recent adjustments to the offset of the high resolution and low resolution sensors and the gain of the high resolution sensor are visible in this menu The offset autocorrection procedure typically takes about 20 minutes and takes place during Sensor Stability the first screen when measur
28. water There is a fill line on the back of the instrument Next set the length of the measuring period in minutes This is the time the F 900 will take to do a measurement When using the water trap a settling time of at least 3 minutes is required before being able to record a measurement At a measuring time of 5 minutes the reading is settled but the overall measurement will take longer A measuring period of 3 5 minutes is sufficient to achieve an accurate reading After 5 minutes you run the risk of reaching saturation Actual times will depend on application environment The length of time required for cleaning will be dependent on the make up of the gas es being sampled The more interfering gases that are present the longer the water will need to be cleaned The main reason for the cleaning step is to keep the water in the maximum adsorption range As the water becomes polluted with alcohols it will not filter as effectively This results in an increase in signal from interfering gasses escaping the filter A small measurement to cleaning time ratio is suggested because the longer the measure time with the filter the longer it will take to clean A recommendation of 4 minutes of measure and 12 minutes of cleaning is better than 20 minutes of measure and 60 minutes of cleaning When the F 900 has conditioning Chamber In turned on the Monitor Mode display top line will indicate CH_IN meaning that Chamber In is enabled Next the current st
29. www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Setup Calibration Current Calibration Parameters and re calibration steps for C2H4 CO2 and 02 sensors can be found in the Calibration Menu Press Enter to confirm and get into the menu The options at the Setup gt Calibration menu are C2H4 CO2 02 system DAC and flow C2H4 Calibration Re calibration or changes to any of the Calibration Parameters for C2H4 must be performed under the supervision of a Felix Instruments technician only The options from the C2H4 Calibration menu are for the C2H4 PPM sensor C2H4 PPB offset autocorrection or sensitivity correction Calibration Parameters are set by a Felix Instruments technician during factory calibration and are specific to each instrument The internal offset is the ADC voltage output from the sensor when no ethylene is present and gain describes the linear increase in the ADC voltage output from the sensor in the presence of ethylene The RH T slope and offset are applied to the ADC voltage to correct for shifts in relative humidity and temperature that may occur during the sample per Calibration Parameters Internal offset Internal gain Calibrated RH Calibrated t RH slope RH offset tslope t offset The ethylene sensor calibration will drift over time Occasional recalibration of the sensors can be carried out using calibration gas in the range of each ethylene sensor For example 1 ppm C2H gas can be used
30. 07 USA Phone 360 833 8835 Fax 360 833 1914 25 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 10 Enter the concentration of the calibration gas in ppb 1 ppm 1000 ppb The concentration of the calibration gas is printed on a tag or sticker on the bottle of gas Press Save to see ethylene value in ppm on display Results Enter Repeat Stop Done peak count 2729747 2 00ppm CO2 0 03 02 21 1 Uptime 00 52 Bat 90 11 Press Enter to repeat and inject a sample Press Stop to exit to main menu Section 3 Sample Injection 12 Follow the same steps but this time inject the sample gas using the same volume of sample as for the calibration gas 13 Choose No by pressing Stop when asked Set as calibration to view the ethylene concentration on the display peak count 2729747 1 92ppm CO2 0 05 02 20 4 Uptime 00 58 Bat 85 14 Press the Enter to start another measurement Press Stop to exit to the Main Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 26 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Section 4 Optional Setting GC Emulation Mode Calibration Manually If performing a calibration injection and verifying the accuracy of the calibration it is no longer necessary to carry out the following steps If questions as to how the calibration is working
31. 1 24 24 1 24 5 24 5 24 6 24 6 24 7 22 9 23 23 1 23 3 23 4 23 3 23 0 23 1 23 8 87 4 87 1 86 8 85 8 85 5 84 3 84 1 42 2 42 1 A 59 3 64 4 66 66 7 67 1 67 2 67 1 67 2 67 2 Figure 13 Example data spreadsheet for a F 900 measurement 101 6 101 6 101 6 101 6 101 6 101 6 101 6 100 4 100 4 100 4 100 4 100 4 100 4 100 4 100 4 100 4 100 4 100 4 100 4 201 REMEMBER Always save the data files to the computer before making changes or starting analysis 1554 NE 3 Ave Camas WA 98607 USA sales felixinstruments com Phone 360 833 8835 Fax 360 833 1914 59 http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Firmware Update Transfer all data files from the SDcard and DELETE all data files on SDcard before doing the firmware update Leaving data files from older firmware versions may corrupt the SDcard Several files should be accessed from the software CD or downloaded from the F 900 software webpage http felixinstruments com support f 900 support software Always double check the webpage for the latest compatible firmware version depending on the serial number of the F 900 Please contact Felix Instruments technical support with questions about firmware updates e Setup application F 900 Package Setup32 exe or F 900 Package Setup64 exe e Firmware code file F 900 ver x xx bin F 900 Driver Installation Procedure 1 Connect the F 900 to the computer usi
32. 1995 Carr J Hampson N The Lead Dioxide Electrode Chemical Reviews volume 72 6 679 703 1972 DOI 10 1021 cr60280a003 Cristescu S Mandon J Arslanov D De Pessemier J Hermans C and F Harren Current methods for detecting ethylene in plants Annals of Botany 2012 DOI 10 1093 aob mcs259 Golding J Shearer D McGlasson W Wyllie S Relationships between Respiration Ethylene and Aroma Production in Ripening Banana Journal of Agriculture and Food Chemistry volume 47 1646 1651 1999 DOI 10 1021 jf980906c Hale C Coombe B Hawker J Effects of ethylene and 2 chloroethylphosphonic Acid on the ripening of grapes Plant Physiology volume 45 5 p 620 1970 DOI http dx doi org 10 1104 pp 45 5 620 Hodgson A Jacquinot P Jordan L and P Hauser Amperometric Gas Sensors of High Sensitivity Electroanalysis volume 11 10 11 1999 DOI 10 1002 SICI 1521 4109 199907 11 10 11 lt 782 AID ELAN782 gt 3 0 C0 2 S Kim G H Wills R Effect of ethylene on storage life of lettuce Journal of the Science of Food and Agriculture volume 69 2 p 197 1995 DOI 10 1002 jsfa 2740690209 Kissinger P Bott A Electrochemistry for the Non Electrochemist Current Separations volume 20 2 51 53 2002 Kou L Turner E Luo Y Extending the Shelf Life of Edible Flowers with Controlled Release of 1 Methylcyclopropene and Modified Atmosphere Packagin
33. 3 8835 Fax 360 833 1914 49 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Setup Board The Setup Board Menu should only be changed by a Felix Instruments technician This menu allows the user the select the type of hardware platform that the unit operates on and should only be changed at installation or if hardware is upgraded Setup Connection Setup gt Connection feature will be fully available in the next version of firmware This feature allows a connection to a computer via USB or WiFi mode Highlight Connect and press Enter to establish a connection Not connected will appear if the connection is not successful Currently the USB connection can be established Mode USB WiFi Group STAB CALI Connect 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 50 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 View Menu The View Menu can be accessed from the main menu screen by pressing the RIGHT arrow key when View is highlighted on the display This menu allows the user to navigate to and see the files as well as check on many features of the F 900 Options from the View menu are Files Voltage Battery Flash Time GPS and SDcard View Files The View Files menu allows the user to review the filenames and file size of any files on the F 900 To view collected and saved dat
34. 4 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 32 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 To dehydrate the sensor place the instrument in closed loop mode with silica gel humidity scrubber in one of the conditioning chambers Enable the chamber with the silica gel and let run overnight Press stop to exit this mode and return to the Setup Sensor Menu Re weight the instrument to determine if more hydration is needed Finally reset the hydration value in the Cell Hydration menu to the current weight minus the shipped weight using the Up Down arrows to adjust the value for weight Current weight original weight RH Sync feature will synchronize the two relative humidity sensors inside the instrument upstream and downstream of the ethylene sensor This should only be done at the instruction of a technician RH reset serves to calibrate the relative humidity sensor This calibration requires N gt gas and should only be done by a technician The calibration has two parts The first part of the calibration will set the zero or baseline for each relative humidity sensor The second part of the calibration will set the span known value for both RH sensors This requires precise control of temperature and humidity of the entire instrument 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 33 sales felixinstruments com http
35. 8607 USA Phone 360 833 8835 Fax 360 833 1914 11 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Charging the Internal Battery The F 900 arrives with a wall charger that can be used to recharge the F 900 battery as well as for continually powering the unit during long term monitoring To charge the F 900 Connect the mini USB cable to the front panel of the F 900 Plug the charger into the wall Check the charge status on off at the View Battery menu Figure 9 The battery charger setup of the F 900 The display will flash powering off on the top line when you hit the power key Power Off will flash on the display as the instrument is powered off For fastest charging of the F 900 use the highest rated port on the charger and do not connect any other USB devices to the charger 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 12 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 To use the F 900 in Monitor Mode to continuously monitor fruit storage rooms or other locations or to run the instrument for days or weeks at a time connect the mini USB cable to the charger cable and to the connection port on the front panel Plug the charger into an electricity source F 900 CO PPM Sensor and Battery Power When the CO2 PPM IRGA sensor is not being actively used it should be turn
36. Also the voltage will begin to increase slowly and the RO will switch to 1d The Board refers to the hardware version of the unit Features described in this manual are associated with all versions of hardware When discrepancies occur they are listed along with the version that they are associated with Other View Menu Options The View Flash option is only accessible by Felix Instruments technicians The View Time screen displays the current time on the instrument Press the LEFT arrow or STOP to exit back to the View Menu The View SDcard screen indicates if an SDcard is present in the unit as well as other information about the SDcard If no SDcard is in the instrument or if the SDcard is not fully inserted no SDcard will appear on the display lt stop gt SDcard Present CardSize 3935MB NumSectors 8058880 BlockSize 512B GPS System Features The F 900 has a built in GPS system which can relay the longitude latitude and altitude of the instrument when performing measurements The View GPS screen displays the mode of the GPS sensor SEN GPGGA as well as the values for latitude longitude altitude and indicates the number of satellites currently connected to Press the UP DOWN arrow to display the latitude longitude and altitude information only lt stop gt lt save gt lt gt SEN GPGGA SAT 05 TIM 015546 806 LAT no fix LON no fix ALT no fix The GPS uses GMT or Gree
37. Chamber In distilled water for most ethylene measurements Place the empty chambers back on the instrument before taking measurements If Chamber In or Chamber Out are selected when the unit enters Closed Loop then they will be included It is recommended to use potassium permanganate KMnO04 in an enabled conditioning chamber when using the closed loop feature MADE i USA Figure 15 Solids Chamber and Water Chamber on back of instrument 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 44 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Chamber Out is on the left of the back panel If the Outlet gas stream is connected to the field kit chamber or to the inlet of the unit it can be used to condition the incoming gas after it leaves the Sensor Enabling Chamber Out the F 900 can use soda lime to scrub CO gt from the gas stream Similarly silica gel is used to scrub water from air and create a 0 relative humidity gas Most commonly potassium permanganate is used to scrub hydrocarbons from the gas stream Potassium permanganate beads not dust should be sourced locally by the user The silica gel has an added color indicator that changes from blue to pink when replacement is necessary Chamber Out is the last chamber before the gas OUT port Chamber Out is primarily used with potassium permanganate KMn04 for cleaning water and setting the instrument
38. Depending on the mode of operation and the environment in which it is used the sensor cell may need to be replenished with distilled water every 2 3 weeks if Chamber In is not set to ON and filled with distilled water The electrolyte level should be monitored on regular basis and prior to operation for an extended period of time The analyzer will arrive filled with the proper electrolyte The refill or replenishment of the sensor is almost fully automated and uses PolarCept Only replenish with distilled water Refilling is needed on a regular basis if the unit is being run with dry gas and Chamber In is not on and filled with water If Chamber In is run continuously with distilled water the level of water in the sensor should not change very much and the levels need only be checked every 6 months Check the sensor every 2 3 weeks if run continuously in very dry environments lt 20 RH It is recommended to refill the sensor before the liquid level drops 20 ml How often the reservoir needs to be refilled will depend on the conditions the instrument is used in temperature relative humidity Note A dry gas being fed into the F 900 at 25 C will cause an expected water loss of 0 1 ml hr at 30 Ca dry gas will cause a loss of 0 3 ml hr Expected water loss can be up to 3 ml day when feeding dry gas through the F 900 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 64 sales felixinstruments com http www felix
39. Full Scale Resolution lt 2 up to 3000ppm Ez aaa i Operating environment 0 C 55 C 5 95 humidity non condensing 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 4 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Theory of Operation The ethylene sensors in the F 900 are electrochemical sensors With this type of sensor the gas sample travels across the surface of the electrode and diffuses into the electrochemical where ethylene is oxidized The current created by the oxidation is measured and converted to parts per million ppm of ethylene Figure 3 Diagram of an electrochemical sensor A working electrode R reference electrode C counter electrode E electrolyte Image credit doi 10 1093 aob mcs259 The F 900 flow rate is regulated by a pump and a flow meter Additional optional sensors are enabled disabled in the Setup Menu see page 31 as well as conditioning chambers on the back of the instrument see page 44 The temperature and relative humidity sensors are located inside the instrument Therefore the temperature sensor is subject to heating by the F 900 internal electronics The temperature reading is often 4 5 C above ambient and in turn relative humidity readings are affected The use of water in Chamber In Polarcept see page 7 will also affect relative humidity readings 1554 NE 3 Ave Camas WA 98607 USA
40. a either download files via USB or eject the SDcard and insert it in a computer In the View Files mode the top line of the display will show the word View on the left and Files on the right Below this the column of the right contains the filename and the column on the right contains the file size The options are use the UP or DOWN arrow keys to scroll through the files press the LEFT arrow or STOP to exit to the View Menu View FileO1 RoomA2 Fruit7 Note A file with a negative number for the file size is corrupt This file should be deleted and re created 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 51 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 View Voltage The View Voltage screen shows the current voltage of the instrument Press the RIGHT arrow when Voltage is highlighted to check the unit s voltage If any of these numbers are rapidly changing after the instrument has warmed up please contact technical support at support Qfelixinstruments com Press the LEFT arrow or STOP to exit back to the View Menu Voltage is set for each individual unit before leaving the Felix Instruments factory and is board specific For default voltage values please see the Production Test Check Sheet at the end of the Instruction Manual for factory voltage settings V_System is the voltage of the control board The voltage for V_Charger
41. a file use the UP or DOWN arrow keys to select a file Once a file is selected to be deleted press the RIGHT arrow key to delete the file Press ENTER to confirm Or to escape without deleting the file press the LEFT arrow or STOP key The display will indicate Done when the file is erased Press STOP or the LEFT arrow to go back to the File Menu Create a File Press the RIGHT arrow when Create is highlighted on the display to get the unit into the create files mode The top line of the screen will read Name on the left and a file name e g file00 on the right The bottom line will read enter at the left stop at the center and the left right arrows appear at the right Name file00 ENTER TO CREATE A FILE STOP TO CANCEL NOTE The STOP key can be pressed at any time to abort the file creation process and to return to this point 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 55 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Pressing the arrow keys will allow the user to enter an alpha or numeric selection for a six character file name The RIGHT LEFT arrow keys select which character in the file name to edit and the UP DOWN arrow keys are used to choose a character The chart lists the available characters Although special characters are seen as options while creating a filename it is cu
42. ample in a syringe the GC Emulation Mode should be used With the simple addition of our inline injection port small volumes of analyte can be accurately measured The use of GC emulation mode requires an ethylene standard preferably in the same concentration range of the sample to be measured f 3 4 mi d H 900 Portable Ethylene Analyzer Figure 13 GC emulation port connected to front of instrument for small volume samples The GC emulation mode employs a patent pending Flow Inject Analysis method to determine ethylene concentrations ranging from 0 5 200ppmv with sample volumes from 5 15ml Just like a GC a simple estimation accurate up to 0 1ppmv is made by comparing the area counts of the unknown sample to a known concentration As shown by Equation 1 the injection volume is determined by the expected concentration of ethylene and the detection limits of the sensor If interfering gases are expected their expected concentrations must be subtracted from the reported ethylene concentration Injection Volume Expected Ethylene Concentration 30mL Exceeding the lower limit of Equation 1 will decrease the accuracy and precision of the result Exceeding the upper limit will affect the sensitivity of the instrument and will cause the instrument to under estimate measurements for up to 24 hours A new calibration point may be required Equation 1 0 2ppmv gt ZO0ppmv 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833
43. as WA 98607 USA Phone 800 767 0119 U S and Canada 360 833 8835 Fax 360 833 1914 Internet http www felixinstruments com E mail support felixinstruments com Customer Service Customer Service Representatives answer questions about specifications and pricing and sell all of the Felix Instruments products Customers sometimes find that they need Felix Instruments to upgrade recalibrate or repair their system In order for Felix Instruments to offer these services the customer must first contact us and obtain a Return Merchandise Authorization RMA number Please contact a customer Service representative for specific instructions when returning a product 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 67 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Frequently Asked Questions If there are any questions about the F 900 please check the Frequently Asked Questions below as well as the Felix Instruments support webpage at http felixinstruments com support f 900 support 1 What type of fruit produces ethylene a Climacteric fruits refer to fruits that have high respiration rate during the fruit s ripening During the ripening process of climacteric fruits the production of a phytohormone ethylene dramatically increases up to 1000 fold of the basal ethylene level Climacteric fruits are ones that are able to ripen after being picked
44. asuring without an SDcard the user must press Enter at the warning screen Pressing any other button will return to the main menu Each time the F 900 tries to autosave the unit will flash write error instead of saved at the top of the display 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 19 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 F 900RK Using the Research Kit Fruit Chamber To use the F 900 Research Kit in the field or laboratory attach a hose of the chamber wand to the IN port on the front panel Slide the end of the wand into the chamber and tighten the thumb screw Make sure the connection is snug The fans inside the chamber will begin to spin when the connection is established The fruit chamber available are 2L in volume The chamber either has a flat bottom or a bottom slit to allow for non destructive sampling A fruit stem or petiole that is attached to the plant is passed through the slit and then closed in the chamber This allows C2H4 levels to be recorded during ripening without damaging the fruit Leaf chambers available for the CI 340 Photosynthesis System are compatible and interchangeable with the F 900 Research Kit If the tube is not connected to the OUT port ambient air will be used to replenish the fruit chamber If the gas returning to the fruit chamber needs to be conditioned consumables should be placed in Chamber
45. ate measure or cleaning will be shown If the F 900 is in state of Measure the data will reflect the current measurement If the F 900 is in a state of Cleaning Clean is shown in the upper right corner and Closed loop at the bottom of the display The F 900 has two hydrophobic filters in line with the internal tubing This is to protect the F 900 in case any water is sucked into the instrument when using PolarCept B_SPEC MEASURE 0 160 ppm HUMIDITY 47 0 TEMPERATURE 28 7C PRESSURE 100 4KPa To avoid drawing water into the internal tubing of the F 900 when using PolarCept 1 Fill conditioning Chamber In only to the fill line and never completely fill the conditioning chamber with water 2 Always keep the top panel of the F 900 facing up and do not tilt the instrument when there is water in the conditioning chamber When operating in SPECIAL mode do not operate the instrument with the display panel facing upwards 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 47 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 3 Do not transport or tip the F 900 if moisture is in Chamber In Before transport or moving the F 900 Chamber In should be completely dry to prevent even small drops of liquid from entering the unit 4 Ifthe Relative Humidity sensor reads high open the top panel of the unit and inspect hoses for liquid If liquid has accumul
46. ated at the hydrophobic blue filter it should be disconnected and drained If liquid has penetrated the hydrophobic filter the unit will need to be serviced to prevent circuit board failure Contact support felixinstruments com with any questions Setup Terminals The Setup gt Terminals Menu is where the F 900 can be setup to have an external fan or gas control connected to the terminal block The terminal block is located between the chambers for consumables on the back panel of the F 900 The F 900 can turn on and off the gas or a fan based on the measured ethylene concentration of the room or chamber In this way the F 900 could be used to control a fan set to evacuate a store room if ethylene concentrations increase past a set point Other control applications using the terminal block are possible such as controlling ethylene scrubbers Setup Terminals VALUE PPB GAS_HI 00000 GAS_LO 00000 FAN_HI 00000 FAN_LO 00000 CONTROL DISABLE Press the UP DOWN arrow to switch between parameters Press the LEFT arrow to switch to the column containing the values in ppb parts per billion Use the UP DOWN arrow keys to set the desired level Press the LEFT arrow STOP or SAVE to exit back to the Setup Menu Any changes made will automatically be saved 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 48 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4
47. card on the computer using Microsoft Excel or Notepad The figure below is an example data spreadsheet Data values included are the date and time of the measurement the ethylene level in ppm the mode monitor measure or cleaning the CO2 and 02 concentration the temperature of the gas stream in degrees Celsius the relative humidity RH of the gas stream in percent the atmospheric pressure KPa and the flow rate of the gas stream in ml min In this example when the CO or 02 sensors are not being used the concentration values are Zero E C D E F G H J Date Time C2H4 ppmv Mode 9 11 2014 9 11 2014 9 11 2014 9 11 2014 9 11 2014 9 11 2014 9 11 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 9 28 2014 16 49 59 16 50 20 16 50 41 16 52 07 16 52 28 16 54 26 16 54 47 14 55 48 14 56 09 14 56 30 15 45 57 15 46 18 15 46 39 15 47 00 15 47 21 15 47 42 15 48 03 15 48 24 15 48 45 0 849 0 843 0 835 0 433 0 796 0 785 0 776 0 0 0 0 796 monitor monitor monitor monitor monitor monitor monitor GC emulation GC emulation GC emulation sm measure sm measure sm measure sm measure sm measure sm clean sm clean sm clean sm clean CO ppm 02 Temp C RH 2 Pressure KPa Flow mL min 1978 1 1656 1411 9 1368 2 1027 6 954 7 872 3 0 0 0 20 37 20 41 20 46 20 43 20 55 20 49 20 54 0 DIE CH 23 5 23 6 23
48. computer application used to view the files can also be used to re save them It is recommended that the user does not work from the original file on the SDcard after the measurement has been made because if any changes are made to the original file it can break the alignment of the file causing error and data loss In order to keep the file system in sync perform any editing or further calculations on the copied file saved to the computer hard drive Do not edit the files directly on the SDcard Do not connect the USB cable to the instrument and the computer while performing measurements If the user tries to operate the instrument through the computer the files are subject to becoming out of sync breaking alignment and causing errors in the files This can also lead to problems with saving data If an error occurs in a specific data file it can be deleted and created again Go to the File Menu on the instrument clear the file where the error has occurred If the file has been transferred to the computer delete it on the computer also Once the corrupted files have been deleted create the file again if it has been properly cleared the same file name can be used and re take the measurement 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 58 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Data Files on the Computer Open the data files saved on the SD
49. concentration gradient Electrochemical Cell sensor DL for bubbling through trap Figure 5 Top diagram of measure period during use of Polarcept Bottom diagram of cleaning period of Polarcept 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 8 sales Qfelixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Unpacking the F 900 The F 900 base unit arrives with the F 900 strap and several accessory parts to use with the instrument Included are the heavy duty USB cable charger tubing accessory kit potassium permanganate KMn04 and an F 900 Instruction Manual Several different styles of connector pieces and various lengths of tubing that do not off gas ex Viton or Teflon are provided as part of the tubing accessory kit Male and female quick connectors can be added to tubing and used to connect sample bags or custom chambers to the F 900 F 900 Portable Ethylene Analyzer Figure 6 Accessories and parts that arrive with the F 900 A F 900 ethylene analyzer B power supply and heavy duty USB cable C Viton tubing D Teflon tubing with quick connects E GC emulation port F assorted quick connects G GC emulation sampling syringe H Silica gel in conditioning chamber 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 9 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015
50. cted on the display Cell Hydration menu features options and details about the water status of the electrochemical sensor The weight is set to 0 00 grams when the electrochemical cell is completely full prior to it being shipped from Felix Instruments As the cell loses water the weight will become a negative value If the weight is greater than 10 0 g the instrument should be hydrated by 10 cc 1cc 1 g A positive value indicates hydration a negative value indicates dehydration of the cell A message on the display to check cell hydration indicates 2 possibilities the ethylene sensor is dehydrate or overhydrated Rehydrating the ethylene sensor is critical to maintain the instrument and accurate readings Cell Hydration Weight g 000 297709 RH sync RH reset When this message appears weigh the entire F 900 with no water or consumables in the conditioning chambers Compare this weight to the weight on the Production Check Sheet on the last page of this manual To rehydrate the electrochemical sensor the instrument should be in the closed loop mode Distilled water should be placed in Chamber IN and it should be enabled Let the instrument run for 12 hours to rehydrate the ethylene sensor Press stop to exit this mode and return to the Setup Sensor Menu Re weigh the instrument to determine if more hydration is needed Always have fresh distilled water in Chamber In when rehydrating the electrochemical cell 155
51. ction Repeated or prolonged inhalation of vapors may lead to chronic respiratory irritation SECTION 4 FIRST AID MEASURES Eye Contact Check for and remove any contact lenses Immediately flush eyes with running water for at least 15 minutes keeping eyelids open Cold water may be used Do not use an eye ointment Seek medical attention Skin Contact If the chemical got onto the clothed portion of the body remove the contaminated clothes as quickly as possible protecting your own hands and body Place the victim under a deluge shower If the chemical got on the victim s exposed skin such as the hands Gently and thoroughly wash the contaminated skin with running water and non abrasive soap Be particularly careful to clean folds crevices creases and groin Cold water may be used If irritation persists seek medical attention Wash contaminated clothing before reusing Serious Skin Contact Wash with a disinfectant soap and cover the contaminated skin with an anti bacterial cream Seek medical attention Inhalation Allow the victim to rest in a well ventilated area Seek immediate medical attention Serious Inhalation Evacuate the victim to a safe area as soon as possible Loosen tight clothing such as a collar tie belt or waistband If breathing is difficult administer oxygen If the victim is not breathing perform mouth to mouth resuscitation WARNING It may be hazardous to the person providing aid to give mouth to mouth resu
52. e F 900 13 How is Chamber In used to rehydrate the electrochemical cell a The electrochemical sensor can be rehydrated by putting distilled water in Chamber In If water is being used in Chamber In for separating out alcohols then refilling of the sensor will never be necessary Similarly if the sensor is used in a humid environment the sensor will not dry out 14 Where are temperature and humidity measured a The temperature and relative humidity sensors are located inside the F 900 before the electrochemical sensor There is an additional relative humidity sensor after the ethylene sensor to help track water loss 15 What type of membrane does the electrochemical sensor have a The electrochemical sensor uses a nafion or nafion like membrane 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 71 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 16 What is the emission rate of ethylene from the fruit taking into account the weight of the fruit or sample and the time measured a Flow rate 0 2L min 15 0L hr Sensitivity to C2H4 0 1 uL L or 100 nL L Therefore the F 900 optimally detects around 1500 nL hr If the specimen weighs 50 grams then the emission rate is 30 nL C2H4 gfw h The unit is nanoliters of ethylene per gram fresh weight per hour 17 What types of tubing materials have been found to off gas a Several types of mate
53. e being made The exterior Inbound and Outbound Chambers are turned On Off valves are closed causing gas to circulate internally Closed Loop On Off or the value displayed by the unit is set to zero set zeros Chamber Out is primarily used with potassium permanganate KMn04 for cleaning water and setting the instrument s offset It is recommended to use Chamber In with distilled water for most ethylene measurements The Set Zeros sets the current value displayed by the instrument to 0 000 ppm The ethylene sensor zero calibration should be performed every 24 hours to compensate for daily baseline drift Toggle between setting the zero for C2H4 CO2 or 02 Use appropriate scrubbers in Chamber Out to set the zero of other sensors CO2 soda lime To perform the C2H4 Set Zero 1 Place potassium permanganate KMnO04 in Chamber Out 2 Enable Chamber Out On and enable Closed loop On 3 Let the F 900 run with KMn0O in the closed loop for 20 30 minutes a After 15 minutes the ethylene concentration of the gas in the closed loop should be close to or equal 0 ppm 4 Press Enter when Set Zeros C2H4 is highlighted to correct the offset baseline shift of the ethylene sensors Press Enter again to confirm the zero calibration 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 17 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Chamber In Water Out Solids
54. e end of a file so files that were previously used can be re opened Open File01 RoomA2 Fruit7 Fruit9 An empty file will have a zero as the file size If the file is corrupt the file size will be a negative number Any file with a negative file size should be deleted and re created Only files with positive numbers or a zero for the file size can be opened to save data too Use the UP or DOWN keys to select the file to open Press the RIGHT arrow to open the file Done will flash across the screen if the file was opened successfully Press STOP or the LEFT arrow to go back to the File Menu Note After a file is created it must be opened in order to start saving data to it 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 57 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Data Transfer to a Computer To view the collected data on a computer remove the SDcard and insert it in an SDcard reader on the computer Or use the supplied USB cable and connect the instrument to the USB host computer The user can only view manipulate data on a computer via the USB connection or with the removable SDcard Data cannot be viewed after it is saved on the F 900 Once the instrument is connected to the computer via USB cable it is strongly recommended that the user copy and re save the files and images to the hard drive of the computer The
55. e value to be falsely high in the presence of interfering gas Felix Instruments has tested a method to absorb some of the competing gases and provide better ethylene measurements This method PolarCept uses distilled water in conditioning Chamber In and has been shown to filter out alcohols and produce less interference It is recommended to always use the PolarCept filter when measuring a gas mixture or interfering gases may be reported by the instrument PolarCept PolarCept is the use of distilled water in conditioning Chamber In to filter out or trap some of the interfering gases Gas from the sample environment is passed through the water trap Some of the polar molecules are held in the trap while ethylene passes through the water trap to the sensor where oxidation occurs CO is released as the oxidation product from the sensor The water being used can become saturated after several minutes so it must be cleaned of the trapped interfering gas molecules Trapped interfering gases are driven out of the water during the cleaning time by a concentration gradient 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 7 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 PolarCept Measure Ethylene Ethanol HA Source of Gases Electrochemical Cell sensor Ispamyl Acetate PolarCept Clean trapped polar molecules are driven out of the trap by a
56. ed off The CO2 PPM sensor draws a lot of power if trying to conserve battery life the sensor should be turned off when not being used The CO2 PPM sensor will draw power when turned on even if the F 900 is not actively measuring Always turn the CO2 PPM sensor to off for the fastest recharge either using the mini USB cable and computer or with a wall charger e The CO PPM sensor ranges from 0 2000 ppm The CO2 PCT ER sensor is from 0 20 and does not have the same power draw To turn off on the CO2 PPM sensor Go to Setup gt Sensor gt Sensor Selection When asked Are you sure you want to continue press Enter Press the DOWN arrow to highlight CO2 IRGA 1 2 3 4 Press the RIGHT arrow key to highlight ON OFF 5 Press the UP DOWN arrow key to toggle ON OFF the sensor 6 Press SAVE when the desired setting is highlighted This will turn off the CO2 sensor until you come back to this menu and turn it back on 7 Ifyou press ENTER the CO sensor setting will only be temporarily saved When the instrument is turned off and then back on the CO2 sensor will be enabled Setup Sensor C2H4 STD On C2H4 ER On C02 IRGA off COZ ER off 02 off 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 13 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Measure Menu Taking a Measurement To begin making measurements select the Measure Menu by
57. ement mode is initiated The default interval between offset autocorrections is 24 hours for continuous use or during the next start up for units that are not running continuously If the unit is exposed to concentrations greater than 200 ppm during the measurement period the offset autocorrection will be initiated the next time the user enters Sensor Stability even if less than 24 hours have passed Adjust enable If Yes is selected the unit will automatically adjust the offset every 24 hours or upon startup if more than 24 hours has passed between uses The offset will be corrected when the user first enters measurement mode and is in the Sensor Stability screen To enable the automatic offset adjustment and to ensure that the latest offset adjustment so that the unit is using the most recent zero change the Adjust enable line to Yes To return to factory calibration parameters select No Requesting If Requesting is reading No while adjust enable is on the unit has recently set the offset and will not enter the offset autocorrection the next time the Sensor Stability screen is entered If Requesting reads Yes the unit will begin resetting the offset the next time measurement mode is initiated and Sensor Stability is entered The offset autocorrection interval can be changed from 24 hours default to any user selected interval in hours Offset Autocorrection Adjust enable
58. en that produces ethylene is Botrytis cinerea c Measuring ethylene concentration in the air of rooms where young apple trees in pots are stored adjacent to refrigerated apple storage rooms in order to assess the safety to young apple trees During the winter months the safe level for storage of first year apple trees is below 50ppm d Commercial apple growers are could monitor the ethylene levels of empty refrigerated rooms before storing bare root nursery trees Rooms should be empty of ethylene before storing nursery trees and often growers have no way to monitor this e Monitoring ethylene emission from industrial sources Ethylene is of interest because it plays a role in atmospheric ozone chemistry This will be dependent on interfering gas and the source of industrial emissions 8 How long does it take to recharge the battery a About four hours The unit may be operated while the battery is charging 9 What happens in the cell runs out of water a Ifthe cell runs out of water the lead electrode could undergo sulfation This would affect the sensitivity by reducing the active area on the lead electrodes It is more likely that a lack of water in the cell decreases the activity of the electrolyte and increases the resistivity of the Nafion membrane Both will affect the background current and response time of the cell 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 70 sales felixinstruments c
59. ensor 64 VJ 66 TN 67 Customer SEVICE ET O 67 FEE ENES e Kette 68 Supporting Sciences EEE DEEE EEE E EEE AEE EENE SEENE EESE EEEE 73 FELIX INSTRUMENTS HARDW ARE WARRANTY eege EENE EEEE TENE EATEN REN EES REETA EE 75 APPENDIX I Material Safety Data Sheet for 2N Sulfuric Acid Solution 76 APPENDIX II Procedure for F 900 Validation Using Fruit rrrrrnnnnrrnnnnnnrrnnnnnnrrnnnnnnrrnnnnnnsrrnnnnssernnnnnnernrnnneernnnnnesrnnnnnssennnnnesennnnnsssennn 81 APPENDIX III Guidelines for Measuring Sample Bags rrrrnnnnrrnnnnnnrrnnnnnnrrnnnnnnrrnnnnnnsrnnnnnnnrnnnnnssennnnnnnsrnnnnnesennnnnesrnnnnnesennnnnesnnnnnssssnnnn 83 F 900 Production Test Check Sheet 85 Warranty Registration Card EEN 87 This instruction manual is written for firmware version 4 5 F 900 Instruction Manual rev 4 2 2015 Introduction Congratulations on the purchase of your new F 900 Portable Ethylene Gas Analyzer The F 900 provides accurate real time measurement of ethylene gas concentrations in a compact instrument suitable for field and laboratory use At the core of the instrument is an electrochemical sensor that offers extraordinary sensitivity to ethylene at levels as low as 0 04 ppm 40 ppb in air Ethylene production is known to increase in response to plant stress and during fruit ripening and maturity and until recently sensitive ethylene measurements were available only through elaborate and expensive gas sampling and gas chromatography analysis The inte
60. etup injection tube then press Enter Uptime 00 13 Bat 98 4 Enable or disable the Water Chamber to increase selectivity of C2H4 measurement Enable the chamber only if CO2 and 02 measurements are NOT involved Chamber In should be filled at least half full with fresh distilled water The distilled water in Chamber In should be replaced with fresh DI water every 10 injections Use Water Chamber C2H4 only Yes Enter No Stop 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 24 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 5 Wait for the environment to stabilize or press Enter to skip and continue to the next step Stabilizing environment Enter Skip C2H4 484ppm CO2 0 03 02 20 6 Humidity 51 3 Temperature 21 4C Flow 202mL Ready in 400 sec Uptime 00 45 Bat 92 Section 2 Calibration Injection 6 Enter the volume of sample gas that will be injected in cc 1 ml 1 cc Recommended sample volume ranges from 10 15 ml Press Save 7 Inject calibration gas then press Enter Remove the syringe from injection port Initial count 1609543 current count 1609383 peak count 1609616 runtime 51 CO2 0 03 02 20 6 Uptime 00 40 Bat 90 8 Wait for processing countdown to complete 9 Setthe sample as your calibration standard by pressing Enter for Yes when asked set as calibration 1554 NE 3 Ave Camas WA 986
61. files This mode allows the erasure of the file contents without changing the file name or set up This feature is useful if it is necessary to do a number of similar measurements The user can take these measurements to a particular file copy the results clear the file and be ready to take a new set of measurements to that file Clear File01 RoomA2 Fruit7 The top line of the display reads Clear on the left and file on the right Filenames are listed on the left and the file size is listed in the right column An empty file will have a zero as the file size Use the UP or DOWN keys to select the file to clear Press the RIGHT arrow to clear the file Press ENTER to confirm The display will indicate Done when the file is erased Press STOP or the LEFT arrow to go back to the File Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 54 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Delete a File Press the RIGHT arrow when Delete is highlighted on the display to get the unit into the delete file mode This mode will erase a file and filename completely from the memory SDcard Inthis mode the top line of the display reads Delete on the top left line and file on the right Below this the filename is listed on the left and the file size is listed on the right Delete FileO1 RoomA2 Fruit7 To delete
62. g Journal of Food Science volume 77 5 p S188 S193 2012 DOI 10 1111 j 1750 3841 2012 02683 x Lee Y Park D Park J Kim Y Fabrication and Optimization of a Nanoporous Platinum Electrode and a Non enzymatic 1554 NE 3 Ave Camas WA 98607 USA sales felixinstruments com Phone 360 833 8835 Fax 360 833 1914 73 http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Glucose Micro sensor on Silicon Sensors 8 Cavendish Banana to Various Storage 6154 6164 2008 Conditions International Journal of Fruit DOI 10 3390 s8096154 Science volume 13 4 p 373 2013 DOI 10 1080 15538362 2013 748378 Liu X and J Li Study on the Electrode Materials of Electrochemical Capacitor Pech J Bouzayen M Latche A Climacteric International Journal of Chemistry volume 3 fruit ripening Ethylene dependent and 2 2011 independent regulation of ripening pathways DOI 10 5539 ijc v3n2p198 in melon fruit Plant Science volume 175 1 2 p 114 2008 Morgan P W Another Look at Interpreting DOI 10 1016 j plantsci 2008 01 003 Research to Manage the Effects of Ethylene in Ambient Air Crop Science volume 51 pages Tseng S Chang P Chou S A Rapid and 903 913 2011 Simple Method for the Determination DOI 10 2135 cropsci2010 05 0280 of Ethephon Residue in Agricultural Products by GC with Headspace Sampling Journal of Obenland D Collin S Sievert J Negm F Food and Drug Analysi
63. g of the electrode This will stop the measurement If the sensor becomes poisoned it will continually auto escape with the error message sensor out of bounds If this happens use potassium permanganate KMnO in Chamber Out and set the instrument to Closed Loop This will begin to clean the gas inside the instrument If possible the F 900 ethylene analyzer should be removed from the high concentration environment Flow Block Error The display may show flow blocked and a very low flow rate less than 40 ml min for several reasons If this message appears on the display of the F 900 ensure that the intake on the front of the F 900 is free from obstruction and not covered If connected to tubing or a fruit chamber disconnect and reconnect the tubing from the In port If using water in Chamber IN removed the top panel of the F 900 and ensure no water has entered the F 900 There is a white hydrophobic filter directly down the flow path Chamber In to be checked for water as well as a hydrophobic filter near the in port on the front of the F 900 If water is found inside the tubing or filters STOP the measurement and allow the instrument to dry out Be careful no further water damage occurs when turning the pump back on No SDcard Present If the autosave is enabled and no SDcard is present the user will be notified by seeing NO SDCARD on the Sensor Status display where the FILE and filename usually are In order to continue me
64. ging any configuration press the STOP key to exit back to the Setup Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 30 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Setup Sensor The Setup Sensor Menu has three options Sensor Selection Sensor Voltammetry and Cell Hydration Sensor Selection allows the user to turn on off different sensors of the F 900 After selecting any of the menu options from the Setup Sensor menu the display will prompt Are you sure you want to continue Press Enter to continue to the selection Not all units are built with all sensors so see the Production Check Sheet at the end of the manual to see which sensors are included in your unit All units are equipped with a High Resolution CH ethylene sensor STD or PPB and an extended range C2Ha ethylene sensor ER or PPM The C2H4 PPB measures from 0 2 ppm The C2H4 PPM measures from 2 200 ppm Optional sensors available for carbon dioxide are the PPM IRGA or the PCT ER which gives values in percent There is also an additional sensor available for O2 percent oxygen Sensor Selection Press the RIGHT arrow when Sensor Selection is highlighted to see the list of sensors Setup Sensor C2H4 STD On C gt Ha ER On CO2 IRGA ER 02 Use the UP DOWN arrows to select the sensor to adjust and then the LEFT RIGHT arrow keys to switch between columns Once in
65. hargeable Li Ion battery provides 4 hours of monitoring in the field 9 gt gt gt gt gt gt e Multiple configurations to accommodate in situ research and controlled atmosphere monitoring Instantaneous and accumulated measurement modes Monitor and Graph View Quick connect sampling ports Data saves in csv commas separated value file Configured for domestic and international operation 110 VAC 60 Hz or 230 VAC 50 Hz Non destructive measurements Mini USB chargeable and mini USB data download GPS and time date stamped data records External wire terminals for fixed location monitoring or control applications 9 gt gt amp gt gt UD GC emulation mode for small sample volumes table Ethylene Analyzer yy Figure 2 The removable SDcard on the F 900 makes data transfer quick and simple 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 2 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Specifications Be JE Open Loop Monitoring 5 sec intervals Sample Rate Closed loop lt 3 min WE SE 5 adjusted for temperature and humidity NG FG 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 3 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Optional Sensors 0 3000ppm Optiona Full Scale Resolution
66. ht it is 2 000 ppm The y axis scale is set by the highest value shown in the buffer This range will scale vertically dependent on the highest concentration of ethylene measured If the concentration is small the dynamic range will reflect this For example the initial value was 0 162 ppm and there is a sudden peak to 7 578 ppm As the spike or peak occurs the graph will scale to 10 000 ppm If the sample returns to 0 168 ppm the scale will remain at 10 000ppm until the graph and the buffer are cleared The scale of the graph can be increased or decreased by using the up and down keys Pressing the up arrow will zoom in on the data minimizing the visible range Using the down key will increase the scale up to 200 ppm Note that the minimum range of data visible down to 0 100 ppm depends on the current data being graphed For example if the current data is 3 486 ppm the user may use the up Key to zoom in to the 5 ppm range but cannot zoom in to a narrower 2 ppm range The graph begins on the left side and moves with the vertical line towards the right as more data points are added Once the time line reaches the right side of the display the data will be compressed to the far left The graph refreshes every 1 hour The time scale relates directly to the vertical position line In other words the line moves to the right as new data points are added Every time a data point is added the time scale is incremented For time under 10 minutes
67. ill Corrosive liquid Stop leak if without risk Absorb with DRY earth sand or other non combustible material Do not get water inside container Do not touch spilled material Use water spray curtain to divert vapor drift Prevent entry into sewers basements or confined areas dike if needed Call for assistance on disposal Neutralize the residue with a dilute solution of sodium carbonate Be careful that the product is not present at a concentration level above TLV Check TLV on the MSDS and with local authorities SECTION 7 HANDLING AND STORAGE Precautions Keep container dry Do not breathe gas fumes vapor spray Never add water to this product In case of insufficient ventilation wear suitable respiratory equipment If ingested seek medical advice immediately and show the container or the label Avoid contact with skin and eyes Keep away from incompatibles such as alkalis May corrode metallic surfaces Store in a metallic or coated fiberboard drum using a strong polyethylene inner package Storage May corrode metallic surfaces Store in a metallic or coated fiberboard drum using a strong polyethylene inner package Corrosive materials should be stored in a separate safety storage cabinet or room SECTION 8 EXPOSURE CONTROLS PERSONAL PROTECTION Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective threshold limit value Personal Protecti
68. ings representations or warranties of any kind express implied statutory or otherwise including but without limitation the implied warranties of merchantability and fitness for a particular purpose not expressly set forth herein 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 75 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 APPENDIX I Material Safety Data Sheet for 2N Sulfuric Acid Solution SECTION 1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION Product Name Sulfuric Acid 2N Catalog Codes CASH Mixture RTECS Not applicable TSCA TSCA 8 b inventory Sulfuric acid Water Cl Not applicable Synonym Chemical Name Not applicable Chemical Formula Not applicable Contact Information Felix Instruments Applied Food Science 1554 NE 3 Ave Camas WA 98607 USA Phone 1 800 767 0119 U S and Canada 1 360 833 8835 CHEMTREC 24HR Emergency Telephone call 1 800 424 9300 International CHEMTREC call 1 703 527 3887 For non emergency assistance call 1 281 441 4400 SECTION 2 COMPOSTION AND INFORMATION ON INGREDIENTS Composition Name CAS by Weight Sulfuric acid 7664 93 9 98 7732 18 5 90 2 Toxicological Data on Ingredients Sulfuric acid ORAL LD50 Acute 2140 mg kg Rat VAPOR LC50 Acute 255 ppm 4 hour s Rat SECTION 3 HAZARDS IDENTIFICATION Potential Acute Health Effects Extremely ha
69. instruments com F 900 Instruction Manual rev 4 2 2015 Use Conditioning Chamber In to Replenish the Sensor RECOMMENDED Conditioning Chamber In in special mode with distilled water can be used to help keep the electrochemical cell hydrated and avoid having to refill the sensor with the syringe method 1 Fill Chamber In to the fill line located on the back of unit using fresh clean distilled water Fill Chamber Out with potassium permanganate Turn on Chamber In and Chamber Out Enable Closed loop Allow the unit to run the closed loop with water in Chamber In for several hours or until the unit has returned to the overall sensor full weight 6 Details about the electrochemical cell status can be found in the Setup gt Sensor gt Cell Hydration menu Ee Note The refill dead time is proportional to the R H of the measured gas and the duration of measurement For example dry gas at lt 10 R H will have 1 1 measurement to dead time Room air at 25 30 R H will have 1 2 ratio Any unit that measures 40 R H should in theory never dry out Note Weighing the entire F 900 unit on an analytical balance when the sensor is full will allow you to monitor the water level of the sensor by keeping track of the weight loss of the whole F 900 unit Remember to always weigh the F 900 with the same setup and to follow the instructions above to remove and refill the sensor when it has lost 20 25 grams of water The weight of the in
70. it has set the zero and the span for the O z sensor the Calibration Results are displayed Press Save to save the values and return to the Calibration Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 42 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Setup Time and Date To change the time and date on the F 900 press the RIGHT key when Time is highlighted on the Setup menu This utility allows the user to set up the instrument in different time zones or to adjust the time after daylight savings To change the time on the instrument use the UP DOWN arrows to change the values Use the LEFT RIGHT arrow to highlight the appropriate column Then make the appropriate shift in time and press SAVE Setup Time 03 28 2013 16 33 24 Setup Flow The air flow of the F 900 is an important factor for taking good measurements The default flow rate is 200 ml min Flow rate is adjustable from 100 500 ml min A flow value below 100 ml min can negatively impact sensor functionality If measuring bottled gas a lower flow rate may be used in order to reduce gas waste If measuring open air a higher flow rate 300 ml min is acceptable The default flow rate is 200 ml min Setup Set Flow Actual Flow To make adjustments 1 Goto Setup gt Flow and press the RIGHT arrow Use the UP DOWN arrows to adjust the value The Set Flow value is found i
71. it in the GC gas chromatograph Record result in spreadsheet program such as Microsoft Excel 6 Vent the fruit chamber record the result in Excel and start method 2 The fruit chamber should be well vented to flush out any plant gases between measurements Method 2 Sampling with Filtration Trapping The purpose of this method is to determine what response the sensor has with trapping of interfering gases by H20 This can be thought of as the effect to the instantaneous measurement Procedure 1 Fill conditioning Chamber In with distilled water and clean the water using potassium permanganate in an in line column connected to the intake port of the F 900 until the background signal is below 20ppb 2 Load the sample specimen into the fruit chamber 3 Start measuring the fruit headspace with the F 900 4 After sampling the headspace for exactly 5 minutes take a syringe sample and run it in the GC gas chromatograph Record result in spreadsheet program such as Microsoft Excel 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 81 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 5 Ventthe fruit chamber record the result in Excel and start method 3 Method 3 Long Term Monitoring with Filtration The purpose of this method is to determine the effectiveness of the PolarCept filter for long term measurements This will require the trap to self rege
72. luded These bags are often used to collect ambient air samples to analyze later in a laboratory using a gas chromatograph or can be connected to the F 900 using the accessory parts Ensure that the bag material and fittings are appropriate for the compounds to be sampled Use only Teflon or Viton tubing to connect the sample bag to the pump to prevent sample loss by adsorption on tubing walls Before using flush the bag thoroughly with purified air or nitrogen Analyze the sample within 24 to 48 hours Long term storage of air contaminant mixtures in bags is not recommended Donot ship sample bags by air unless the cargo cabin is pressurized Do not overfill sample bags Secure polypropylene value when opening closing by holding side stem while turning entire upper portion of fitting one revolution Store bags flat Do not roll or crease bags during storage Donot use bags at temperatures above 140 F 60 C Bags are designed for single use only To Collect a Sample Using a Sample Bag 1 Flush the bag at least 3 times with purified air or nitrogen before use 2 To fill a bag connect tubing from the exhaust port of an air sample pump to the hose connection on the bag stem protruding from the side of the fittings 3 To open the shut off valve hold the side stem and turn the entire upper portion of the fitting including the brown syringe port and the white section to which it is attached co
73. n ml min to the far right The Actual Flow value will change to match the set flow Press SAVE to save changes and exit back to the Setup Menu ER SN Press STOP to exit back to the Setup Menu without saving 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 43 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Setup Chamber To access the Setup Chamber feature of the F 900 press the RIGHT arrow key when chamber is highlighted on the Setup Menu Here the user can turn on off the in line conditioning columns that are on the back of the instrument The settings in Setup Chamber are the default settings to start a measurement Once a measurement is in process conditioning chambers can be turned on off in the Measurement Settings menu The removable plastic containers are used to hold the consumables soda lime silica gel potassium permanganate and distilled water The purpose of the inbound chamber Chamber In is to condition the air before it reaches the sensor Chamber In additionally provides a special mode where distilled water is used to filter out alcohols before the electrochemical sensor The use of distilled water in the Chamber In is referred to as PolarCept The purpose of the outbound chamber Chamber Out Solids is to condition the air going back into the fruit chamber or room and occurs after the sensor It is recommended to use PolarCept
74. nd seals are not over pressurized Use the regulator on the gas tank to set the flow to just over 1 PSI This will ensure the unit is provided with a bit more than the necessary gas for it to operate at 200 ml min wasting calibration gas will happen at over 2 PSI If N2 gas is not accessible use the Soda Lime provided with the research kit Create a 0 ppm CO environment by filling the external conditioning tube with soda lime and connecting it to the inlet This will generate CO free air allowing the instrument to set the zero as it would with the N gt gas 4 Once the N2 gas or soda lime is connected press Enter The instrument will enter a screen that reads Internal Offset and count down from 99 5 When the offset is obtained the instrument will pause and allow you to calibrate to either the 20 range or 100 range For example if you need the instrument to read 50 press Save to calibrate to the 100 range 6 Inthe Setup Gas screen connect the CO2 use a T connection to not over pressurize the instrument and change the value to 00050 Press Save 7 The instrument will now set the internal gain and count down from about 46 8 The final calibration values will then be displayed Press Save to save the values completing the calibration and returning to the Calibration Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 38 sales felixinstruments com http www
75. nerate between consecutive measurements The regeneration time is defaulted to 6 minutes however for some species a greater time may be needed By keeping the default value for avocados we can better compare results and will demonstrate the weakness of the method Procedure 1 Fill conditioning Chamber In with distilled water and clean the water using potassium permanganate in an in line column connected to the intake port of the F 900 until the background signal is below 20ppb 2 Verify that Chamber In is set to special with a sufficient cleaning period for the produce being monitored 3 Load the sample specimen into the fruit chamber 4 Start measuring the fruit headspace with the F 900 5 Allow the system to run for 5 measurement and cleaning cycles 6 Vent the chamber and load the data file to a computer 7 Switch to the next replicate and begin at method 1 again Outside AIR gt me p H Chamber VENT Cl 900 flow 200mL min Figure 14 Set up for fruit chamber using KMnO1 to scrub ethylene from the outside air 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 82 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 APPENDIX III Guidelines for Measuring Sample Bags The following guidelines are for collecting air samples in standard samples bags In order to collect an air sample you must pump air into the bag pump not inc
76. ng the mini USB to USB cable Launch F 900_Package_Setup32 exe or F 900_Package_Setup64 exe under Windows 64 bit 3 Set up device as prompted message below HA F 900 1 0 Setup Setting up your F 900 Power on the F 900 Connect the F 900 to your Computer via USB port Device Options Help Ed Driver C WinUSB v6 1 7600 16385 More Information WinUSB flibusbx USB ID libusb win32 i libusbk E WEID WinlJSB Microsoft 0 devices found 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 60 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 5 Inthe dropdown list select CI DFU Interface 1 device Driver libusb0 v1 2 6 0 USB ID 0485 5720 wc A 7 devices found Zadig v2 0 1 160 6 Atthe spinner list green arrow select libusb win32 Device Options Help sat Driver libusb v1 2 6 0 C libusb win32 v1 2 6 0 S More Information USED 093 570 01 wcp X 7 devices found fadig v2 0 1 160 7 Click Install Driver to proceed installing libusb driver This could take up to 1 minute to complete Note Wait for Windows obtaining driver process to complete before clicking Install driver Installing device driver software CI DFU Searching Windows Update Obtaining device driver software from Windows might take a while Ski dre Windows Updat 1554 NE 3 Ave Cama
77. nt Also using the water trap can help keep the electrochemical cell hydrated with longer periods of time between needing to refill the electrochemical sensor There are 3 options for Chamber In in the Setup gt Chamber menu 1 Chamber In set to OFF 2 Chamber In set to ON 3 Chamber In set to SPECIAL Use the UP DOWN arrows to select Chamber Out Chamber In or Measure or Cleaning Then use the RIGHT arrow to get to Off On Next use the UP DOWN arrow to switch between on or off or set the measure or cleaning period in minutes Chamber Menu CHAMBER A OUT Off CHAMBER_B IN SPECIAL MEASURE PERIOD 3 MIN CLEANING PERIOD 17 MIN To use soda lime or silica gel to condition the air stream before it is measured turn Chamber In to ON and place the consumable in the conditioning chamber Fill Chamber In less than half full with distilled water The water level of Chamber In should to be inspected every 120 hours when using PolarCept This mode may not be applicable at high ethylene concentrations When the F 900 is using the water to filter out alcohol the water must be cleaned by running a longer cleaning period than the measure period 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 46 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 To use the PolarCept water trap set Chamber In to SPECIAL Fill Chamber In less than half full with distilled
78. nt of the F 900 s portable design is to aid the research community in developing real world ethylene management applications for the benefit of growers processors and consumers The F 900 analyzes the concentration of ethylene in a gas sample for the purpose of research or ethylene management The F 900 employs electro chemical sensors as well as several other sensors for temperature humidity GPS location and optionally carbon dioxide C02 high and low range and oxygen 02 During a sample the subject gas travels across the surface of the electrode and diffuses into the electrochemical cell where ethylene is oxidized The current created by the oxidation is measured and interpreted as the concentration of ethylene reported in parts per million or ppm We hope you enjoy using your F 900 Portable Ethylene Gas Analyzer Figure 1 The F 900 Portable Ethylene Analyzer front panel and display 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 1 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Features High sensitivity to C2H4 0 ppm 200 ppm CO and O2 sensing capability optional Compact portable form factor 2 43Kg Real time continuous monitoring Internal data logging and storage 4GB SD card included Automatic data logging with adjustable interval User adjustable flow rate Transflective display enables easy viewing in direct sunlight Rec
79. nwich Mean Time for a standardized time The GMT is displayed as the time TIM on the GPS menu and is synchronized with minute and second alignment Often when the instrument is turned on indoors the latitude longitude and altitude will read no fix This occurs when no Satellite signal can be obtained by the instrument Powering up the F 900 outdoors will correct this problem and allow latitude longitude and altitude readings to be taken 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 53 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 File Menu The File Menu is accessed by highlighting File on the Main Menu and pressing the RIGHT arrow key Here the user can manipulate files on the F 900 with options to clear delete create and open files The F 900 uses an SDcard to store all measurements This means the user is able to create clear and delete any measurement file To view data on a computer simply insert the SDcard into the computer s SDcard reader The computer should automatically detect the SDcard as a new storage device and mount the drive so that measurement data will be accessible by any computer application The mini USB port can also be used to establish a USB connection with a computer to transfer data from the F 900 Clear a File Pressing the RIGHT arrow key when Clear is highlighted on the display enters the menu to clear
80. om http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 10 What are the advantages of the F 900RK over the standard F 900 a Both are portable but the research kit includes the other components as listed in the Unpacking the F 900 section of the User Manual such as the fruit chamber and wand and hard sided wheeled carrying case The Research Kit is designed to make measurement of fruits non destructively or in a closed chamber Fans are built into the fruit chamber to mix the air 11 What are the most commons uses for Chamber In and Chamber Out a Both chambers are optional but it is recommended to use Chamber In as PolarCept for most measurements The most common use for Chamber In is to separate light hydrocarbons such as alcohol from the gas stream which may interfere with the measurement The most common use for Chamber Out is to be filled with potassium permanganate so that it cleans all ethylene and other hydrocarbons from the gas stream so that any ethylene that is measured must have come from the sample Chamber OUT is also commonly used with KMnO to calibrate the zero of the ethylene sensors 12 During a monitoring experiment will the sealed fruit chamber influence the result as time goes on a The air should be scrubbed by enabling conditioning Chamber Out filled with potassium permanganate Attach the tubing from the wand that returns to the fruit chamber to the Out port on the front of th
81. on Face shield Full suit Vapor respirator Be sure to use an approved certified respirator or equivalent Gloves Boots Personal Protection in Case of a Large Spill Splash goggles Full suit Vapor respirator Boots Gloves A self contained breathing apparatus should be used to avoid inhalation of the product Suggested protective clothing might not be sufficient consult a specialist BEFORE handling this product Exposure Limits Sulfuric acid TWA 1 STEL 3 mg m3 from ACGIH Consult local authorities for acceptable exposure limits 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 78 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical state and appearance Liquid Odor Odorless Taste Not available Molecular Weight Not applicable Color Clear Colorless pH 1 soln water 2 Acidic Boiling Point The lowest known value is 100 C 212 F Water Melting Point Not available Critical Temperature Not available Specific Gravity The only known value is 1 Water 1 Water Vapor Pressure The highest known value is 17 535 mm of Hg 20 C Water Vapor Density The highest known value is 0 62 Air 1 Water Volatility Not available Odor Threshold Not available Water Oil Dist Coefficient The product is much more soluble in water lonicity in Water Not available
82. p Menu The F 900 has a number of utility functions that allow the user to manage the instrument s capabilities These functions are accessed by pressing the RIGHT arrow when Setup is highlighted on the Main Menu The setup utility options are Measure Autosave Sensor Time Flow Chamber Terminals and Board Use the UP DOWN arrows to select the desired option and then press the RIGHT arrow to enter the choice Press the LEFT arrow to exit to the Main Menu Setup Measure Autosave Sensor Calibration Time Flow Chamber Terminals Board Connection Setup Measure The Setup Measure menu allows the user to setup a measurement in 1 of 2 different modes Monitor Mode or GC Emulation Mode Instructions to use Monitor Mode are found on page 16 GC Emulation Mode is available with the F 900 Research Kit and requires the use of the inline injection port with septum To save changes in the Setup Measure menu press SAVE Use the UP and DOWN arrows to toggle between options Setup Autosave To select the Autosave feature press the RIGHT arrow key when Autosave is highlighted This allows the user to setup the instrument to automatically store measurements and to start a new measurement without explicitly saving the old one This mode is convenient when taking fast repetitive measurements as well as when it is not necessary to review each measurement immediately after taking it The files created by the F 900 are saved in csv comma separated
83. r the CO gt sensor zero calibration with the following changes 1 Use 0 ppm O gas 2 Highlight O2 in the Measure gt Settings menu and press ENTER twice when it is time to set the zero Calibrating the 02 sensor with standardized gas is fairly simple Remember to always use a T connection to vent excess gas and prevent damage to the internal components 1 Goto Calibration hit enter then 02 2 Go to Re calibration and press the right arrow You will hear the valves open and the pump begin to run 3 Use pressurized N to create zero 02 gas When the gas is flowing and connected to the unit press Enter 4 The unit will count down from as it sets the zero for the 02 sensor The humidity Temperature and Flow are displayed The flow should continue to read 200 mL throughout the calibration if it does not you may have a leak or obstructed tube Pressing enter at any time will move you into the next step Only do so if you want to use the previous zero calibration 5 Setup Calibration gas Enter the percent concentration where 1000 E 3 100 and 210 E 3 21 Use the LEFT RIGHT arrow keys to switch between placeholders and the UP DOWN arrows to change the values 6 Press save to initiate the calibration 7 The unit will count down from 19 as it sets the span for the O2 sensor Pressing enter at any time will move you into the next step Only do so if you want to use the previous span calibration 8 After the un
84. rial have been found to off gas interfering gases of the ethylene sensor such as Tygon Viton and Teflon have successfully been found to not off gas 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 72 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Supporting Sciences References The following list of citations represent current peer reviewed and other literature concerning electrochemical sensors the study of climatic fruit and ethylene Banks N Cleland D Cameron A Beaudry R Kader A Proposal for a Rationalized System of Units for Postharvest Research in Gas Exchange HortScience volume 30 6 p 1129 1131 1995 Bellincontro A Fardelli A De Santis D Botondi R Mencarelli F Postharvest ethylene and 1 MCP treatments both affect phenols anthocyanins and aromatic quality of Aleatico grapes and wine Australian Journal of Grape and Wine Research volume 12 2 p 141 149 2006 DOI 10 1111 j 1755 0238 2006 tb00054 x Both V Brackmann A Thewes F Ferreira D Wagner R Effect of storage under extremely low oxygen on the volatile composition of Royal Gala apples Food Chemistry volume 156 1 pages 50 57 2014 DOI http dx doi org 10 1016 j foodchem 2 014 01 094 Bott A Practical Problems in Voltammetry 3 Reference Electrodes for Voltammetry Current Separations volume 14 2
85. rrently recommended not to use special characters when naming files with firmware version 1 07 The filename must end in a number or else the file will be corrupt and no data will be saved to it ABCDEFGHIJKLMNOPQRSTUVWXYZ abcdefghijklmnopgqrstuvwxyz 0123456789 Pressing the SAVE key in the Create mode deletes the character under the cursor Note that characters cannot be inserted into an existing filename Should the file name be identical to another file name the instrument will display the message duplicate name on the top line of the display and enter on the bottom Fruit01 Create lt enter gt lt stop gt The F 900 will save all parameters for each measurement on the SDcard Once finished creating the file name press the ENTER key or press STOP to abort the process The F 900 will exit to the File Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 56 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Open File Press the RIGHT arrow when Open is highlighted on the display to get the unit into the open files mode The top line of the screen will read Open on the left and File on the right Filenames are listed on the left and the file size is listed in the right column The default file on the instrument is File01 Data will be saved into File01 unless another file is created and opened Data can be appended to th
86. s WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 61 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 8 Device drivers and Software package installation is done Under Windows Start menu you should be able to find shortcuts for Device s Controller and Firmware Upgrade Program A Felix Instruments rn F 900 Controller ch F 900 Firmware Upgrade F 900 Firmware Update Procedure CT S00 opened 1 After installing the driver open the F 900 Firmware ee EE Upgrade application Transfer Code to CI 203 or CI 900 2 Select File and Open a 3 Navigate to the F 900 ver x xx bin This is the code for the instrument 4 Follow the instructions in the software to hold down the power button 5 Release the power button when instructed 6 Wait for the flash to be erased and then written When the firmware transfer is complete the software will indicate reset sent followed by leaving open The F 900 will power off 7 Power on the F 900 and check the firmware version at the top of the display The Transfer Code software application will indicate that no instrument is found if the F 900 isnot connected to the computer isnot powered on driver is not properly installed No instrument found Remove then restore cable Leaving OPEN 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 62 sales felixinstruments com
87. s volume 8 3 213 Arpaia M Influence of maturity and ripening 217 2000 on aroma volatiles and flavor in Hass DOI 20003023586 avocado Postharvest Biology and Technology volume 71 41 50 2012 Vigier F Coutanceau C Perrard A Belgsir DOI http dx doi org 10 1016 j postharvbio E Lamy C Development of anode catalysts 2012 03 006 for a direct ethanol fuel cell Journal of Applied Electrochemistry volume 34 439 Opara U Al Yahyai R Al Waili N Al Said F 446 2004 Al Anj M Manickayasagan A Al Mahdouri DOI 10 1023 B JACH 0000016629 98535 ad A Postharvest Responses of Malindi 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 74 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 FELIX INSTRUMENTS HARDWARE WARRANTY Important Please Read Seller s Warranty and Liability Seller warrants new equipment of its own manufacturing against defective workmanship and materials for a period of one year of a single shift operation from date of receipt of equipment the results of ordinary wear and tear neglect misuse accident and excessive deterioration due to corrosion from any cause is not to be considered a defect Any defect must be called to the attention of Felix Instruments Camas Washington USA in writing within 90 days after receipt of the unit Seller s liability for defective parts is limited to
88. s offset To use Chamber Out to condition the air replenishing the fruit chamber enable Chamber Out in the Setup gt Chamber menu and fill Chamber Out with the appropriate consumable Connect the fruit chamber hoses to both the IN and OUT ports on the front of the F 900 Chamber In Chamber Water Out Solids A neh Flow Meter NA f t RH In Temperature Intake Outlet fr Figure 16 The back of the F 900 with removable and refillable containers for distilled water and KMnO left and a diagram showing the flow path of the gas stream right Phone 360 833 8835 Fax 360 833 1914 45 http www felixinstruments com 1554 NE 3 Ave Camas WA 98607 USA sales felixinstruments com F 900 Instruction Manual rev 4 2 2015 Keep the plastic containers on the F 900 even when empty to protect the brass intakes Always ensure the conditioning chamber is seated properly when screwing it on the F 900 Chamber In is on the right side of the back panel This chamber can be enabled in 2 different modes Chamber In should be filled with distilled water to help filter out interfering gases in the air stream By passing the gas sample through the water polar molecules are trapped or filtered from the airstream Using Chamber In with distilled water as a filter can help reduce the interference measured when alcohol or other light polar molecules known interfering gases for the F 900 electrochemical sensor are prese
89. scitation when the inhaled material is toxic infectious or corrosive Seek immediate medical attention Ingestion Do not induce vomiting Loosen tight clothing such as a collar tie belt or waistband If the victim is not breathing perform mouth to mouth resuscitation Seek immediate medical attention Serious Ingestion Not available SECTION 5 FIRE AND EXPLOSION DATA Flammability of the Product Non flammable Auto Ignition Temperature Not applicable Flash Points Not applicable Flammable Limits Not applicable Products of Combustion Not available Fire Hazards in Presence of Various Substances Not applicable Explosion Hazards in Presence of Various Substances Risks of explosion of the product in presence of mechanical impact Not available Risks of explosion of the product in presence of static discharge Not available Fire Fighting Media and Instructions Not applicable Special Remarks on Fire Hazards Not available Special Remarks on Explosion Hazards Not available 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 77 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 SECTION 6 ACCIDENTAL RELEASE MEASURES Small Spill Dilute with water and mop up or absorb with an inert dry material and place in an appropriate waste disposal container If necessary Neutralize the residue with a dilute solution of sodium carbonate Large Sp
90. ser presses the right arrow to enter Monitor View Press the DOWN arrow key to switch between automatic and manual initialization Press the RIGHT arrow to skip the Sensor Stability screen and proceed to the Graph View display If the offset autocorrection feature is enabled then the unit will run the set zero process automatically every 24 hours or at the user defined time interval The offset autocorrection utilizes Chamber Out which should be left full of KMnO even if Chamber Out is set to off during normal measurements For more details on the offset autocorrection see the Setup gt Calibration menu on page 34 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 14 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Graph Mode Graph Mode will be entered automatically when the unit has stabilized in Sensor Stability mode Or to enter Graph Mode press the RIGHT arrow when the F 900 is in Sensor Stability mode This view graphically displays the measured C2H value over time To switch Graph Mode into Monitor Mode press the right arrow To exit the measurement press stop GRAPH MODE 00 09 35 C2H4 0 205 ppm Uptime 14 52 13 Bat 98 Figure 10 Graph Mode with red arrow indicating direction of movement Directly below the graph is the current ethylene value Above the graph is the dynamic range on the left it is 1 000 ppm on the rig
91. strument completely assembled with a full sensor is found in the Production Test Check Sheet 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 65 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Warnings Read this manual carefully before using this manual should be read by anyone who will be operating the F 900 By following the guidelines the F 900 will function according to the specifications in this instruction manual All repairs should be performed by a Felix Instruments technician The analyzer must be used to measure the rate of ethylene in the presence of nitrogen oxygen and carbon dioxide All other gases in high concentration could lead to erroneous measurements or the destruction of the sensor Do not use the instrument in the presence high concentrations of aromatic solvents hydrocarbons or other volatile organic compounds such as in unventilated painting rooms or chemical storage rooms Damage to the analyzer can occur if the fluid reservoir level is too low or if the inlet and outlet gas connections are blocked while the pump operates This can cause false readings or irreparable damage to the sensor Ifsmoke comes out of the analyzer turn off the power immediately and contact Felix Instruments Applied Food Science Keep the analyzer in a clean and ventilated room at ambient temperature Do not allow fluid to aspira
92. te in the analyzer which can destroy the sensors Do not use the F 900 in a high RF radio frequency environment High RF will disrupt the F 900 ESD electrostatic discharge can also interfere with F 900 Do not tilt the F 900 when Chamber In contains water The analyzer has a reservoir containing 1 mol of sulfuric acid 2N H2S04 Avoid contact with skin and eyes If contact occurs rinse thoroughly with water or use an eyewash or safety shower Do not drink the contents of the fluid reservoirs as it could cause chemical burns See Appendix I for the safety data sheet and further information on sulfuric acid 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 66 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Technical Support If you have a question about the F 900 first look in the F 900 Operation Manual There is also online support available for the F 900 at http felixinstruments com support f 900 support If you cannot find the answer you can contact a Technical Support Representative located in your country Felix Instruments is committed to provide customers with high quality timely technical support Technical support representatives are to answer your technical questions by phone or by e mail at support felixinstruments com Felix Instruments contact information Felix Instruments Applied Food Science 1554 NE 3rd Ave Cam
93. ted inside the instrument Therefore the temperature sensor is subject to heating by the F 900 internal electronics The temperature reading is often 4 5 C above ambient and in turn relative humidity readings are affected The use of water in Chamber In Polarcept see page 7 will also affect relative humidity readings The detection rate Det Rate in nmol second is calculated by the following formula based on ethylene being an ideal gas e Detection rate n V P R T e Where volume V flow rate concentration L s ppb nL s e P pressure atm kPa 0 0098692 e R constant 0 0821 e T absolute t K 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 16 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 The detection rate starts calculating the ethylene emission rate from the fruit Emission rate is typically calculated with the following parameters and the weight of the fruit e Flow rate 0 2L min 15 0L hr e Sensitivity to C2H4 0 1 uL L or 100 nL L Therefore the F 900 optimally detects around 1500 nL hr If the specimen weighs 50 grams then the emission rate is 30 nL C2H4 gfw h The unit is nanoliters of ethylene per gram fresh weight per hour Measurement Settings The Settings screen is accessed by using the RIGHT arrow from Monitor Mode and provides a short list of variables which may be manipulated while measurements ar
94. the column with the On Off use the UP DOWN arrow to turn the sensor On Off Both ethylene sensors should always be turned On for measurements The firmware will control which ethylene sensor is used to measure the gas sample Ifsensors are not included in your instrument please set them to Off If not actively using the CO2 PPM IRGA sensor set it to Off to conserve battery life Press SAVE to save any changes Press STOP to exit to the Setup Menu 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 31 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Sensor Voltammetry should only be used if instructed by a CID technician Sensor voltammetry is the electrode recovery mode and is a useful tool if the electrochemical cell has been poisoned However running Sensor Voltammetry may cause the calibration to shift changing the data displayed on the screen During this process the pump will be turned off and a series of voltages changes will occur on the sensor First voltage changes serve to oxidize anything on the electrode followed by reduction and finally a re stabilization period for the sensor The cleaning will take about 40 minutes to complete and the sensor may be very sensitive and noisy for a period of time following the cleaning If Sensor Voltammetry is selected by mistake please press the STOP key to exit as instru
95. the graph is updating almost every second The bottom of the display shows the uptime of the F 900 and current battery level 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 15 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Monitor Mode MONITOR 3 26 ppm Humidity 47 0 Temperature 28 76 Pressure 101 0 kPa Flow 200 mL Det Rate 0 03 nmol s Uptime 01 17 Bat 95 The large bold value on the top line is the digitally filtered value of the raw ethylene measurements Next the HUMIDITY or relative humidity in percent of the gas stream in the instrument is displayed followed by the TEMPERATURE in degrees The PRESSURE or atmospheric pressure is displayed Press the DOWN arrow to view the FLOW in mL per minutes The default flow rate is 200 mL per minute If the unit has a high resolution CO2 sensor on board the CO2 value in ppm will be displayed below the ethylene value If the unit has a low resolution COz sensor or 02 sensor on board the value will be displayed in percentage If Autosave is enabled saved will flash at the top of the display each time the data is automatically saved to the file Press the STOP key to stop the measurement and exit to the Sensor Status display Press the LEFT arrow to enter GRAPH Mode Press the RIGHT arrow to get to the Measurement Settings menu The temperature and relative humidity sensors are loca
96. u first learn of this product o Advertisement in o Representative o Friend Colleague o Exhibit o Other 4 Who selected this product oI did o Research Group o University Department o Purchasing o Other 5 Comments Suggestions 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 87 sales felixinstruments com http www felixinstruments com 5
97. unterclockwise one revolution Turn on the pump and sample 4 Avoid filling any bag more than 80 of its maximum volume 5 When sampling is complete turn off the pump To close the shut off valve hold the side stem and turn the entire upper portion of the fitting clockwise until it is snug 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 83 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 6 To withdraw samples using a needle and syringe carefully insert the needle into the septum port in the center of the brown cap and pierce the septum Do not allow the needle to puncture the bag material when piercing the septum 7 Do not use the fitting valve as a handle or hanging device Although it is durable it is not intended for these uses Analyzing a Sample Bag with the F 900 Connect the sample bag to the IN port of the F 900 Open the sample bag to allow gas to start flowing to the F 900 Ittakes about 3 5 minutes to measure a sample bag if the concentration of ethylene before measuring the sample bag is low 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 84 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 F 900 Production Test Check Sheet SERIAL NUMBER C24 STANDARD E coud Er r 900 fully assembled 0 Z o o o
98. ved certified respirator or equivalent Wear appropriate respirator when ventilation is inadequate Face shield SECTION 16 OTHER INFORMATION References Not available Other Special Considerations Not available Created 12 21 2010 Last Updated 4 23 2012 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 80 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 APPENDIX II Procedure for F 900 Validation Using Fruit The following methods should be run consecutively on a given replicate and repeated for each new replicate new specimen At least 5 replicates for pre climacteric un gassed or still green apples bananas or avocados should be run The data from method 1 and 2 can be combined into a table The results from method 3 can be graphed as a figure to show the trend over time Method 1 Sampling with No Filtration Trapping The purpose of this method is to determine what response the sensor has without trapping of interfering gases This can be thought of as the baseline response Procedure 1 Turn on the F 900 verify chambers are set to off and flow is set to 200 ml min 2 Connect experimental setup as show in Figure 14 3 Load the sample specimen into the fruit chamber 4 Start measuring the fruit headspace with the F 900 5 After sampling the headspace for 5 minutes or a set period of time of your choosing take a syringe sample and run
99. your computer To change the separator in all csv text files 1 In Microsoft Windows click the Start button and then click Control Panel 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 68 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 2 Open the Regional and Language Options dialog box 3 Do one of the following a In Windows Vista and 7 click the Formats tab and then click Customize this format b In Windows XP click the Regional Options tab and then click Customize 4 Type a new separator in the List separator box For example type 5 Click OK twice NOTE After you change the list separator character for your computer all programs use the new character as a list separator You can change the character back to the default character by following the same procedure 5 How do I create and open a file to start using the F 900 a To start using the F 900 create a file to save data into Go to File gt Create Change the file00 to the desired file name and press save Next open the file to Save measurements Go to File gt Open and use the arrows to highlight the newly created file If the value next to the file name is a negative number this indicates an error You should delete the file and create it again Then open it and check that the value is zero Now you can save data into this file 6 What consumables come with the F
100. zardous in case of skin contact corrosive irritant of eye contact irritant of ingestion of inhalation Liquid or spray mist may produce tissue damage particularly on mucous membranes of eyes mouth and respiratory tract Skin contact may produce burns Inhalation of the spray mist may produce severe irritation of respiratory tract characterized by coughing choking or shortness of breath Inflammation of the eye is characterized by redness watering and itching Skin inflammation is characterized by itching scaling reddening or occasionally blistering 1554 NE 3 Ave Camas WA 98607 USA Phone 360 833 8835 Fax 360 833 1914 76 sales felixinstruments com http www felixinstruments com F 900 Instruction Manual rev 4 2 2015 Potential Chronic Health Effects Extremely hazardous in case of skin contact corrosive irritant of eye contact irritant of ingestion of inhalation Nonsensitizer for skin Non permeator by skin CARCINOGENIC EFFECTS Not available MUTAGENIC EFFECTS Not available TERATOGENIC EFFECTS Not available DEVELOPMENTAL TOXICITY Not available The substance is toxic to lungs mucous membranes Repeated or prolonged exposure to the substance can produce target organs damage Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation Repeated or prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infe

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