Essential Information for the Aerospray® Gram Slide Stainer
Contents
1. 1 Disassemble and clean all nozzles Do not mix or interchange nozzles or nozzle parts 2 Remove Pick up Tube from Reagent B lodine bottle Then A Flush 500 mL DI water through the line B Flush 100 mL prepared SS 029C through the line Block flow at nozzle holder with a gloved finger for a few seconds during flushing Stop flushing just before 100 mL is gone Soak in line 1 hour to overnight as practical C Flush prepared SS 029C out of line with 500 mL of DI water D Reinstall Reagent B lodine and flush 100 mL through the line to remove DI water 3 Reinstall nozzles Always return nozzles to the same location a 2B o IS 4 Perform SPRAY PATTERN and SPRAY VOLUME tests EN Record results from end of month SPRAY VOLUME test on 30 P preventive maintenance chart NOTE As you enter a new month the Volume After Monthly Cleaning number becomes A A A Previous Months Ending Volume number 5 Disinfect reusable bottles with a 1 10 dilution of bleach Rinse Reagent Lot Record Corrective Action Log Reagent Reagent Reagent C D E Reagent Reagent B gt NININININ Ia a a a a a aja aj J NINNIN thoroughly with deionized water Instructions for Reagent Lot Record Corrective Action Log Instructions B The Corrective Action Log is optional Document each sa ae aa oes PM Each time you replace a reagent bottle record lot number from the problem occurrence ae 1 the date 2 a brief ANNUAL a CMA new2. m Procedure for Use 8 7g Refer to Operating Instructions in Section 6f n Internal Quality Control 8 7k Refer to Spray Pattern Test Spray Volume Slide Pattern Test and Interpreting Results in Section 6 o Performance Verification Maintenance and Safe Waste Disposal 8 7n I Performance Verification To verify performances see the Spray Pattern Test Spray Volume Test and Interpreting Results in Section 6 Il Maintenance The Aerospray stainer requires little maintenance To help document the maintenance use the following procedures and Preventive Maintenance chart DAILY At end of each shift or if instrument will be idle for more than 8 hours 1 Runa CLEAN cycle Leave instrument in CLEAN TO REPRIME 2 Use a spray bottle filled with methanol or ethanol spray the front of each nozzle and clean each nozzle orifice with nozzle brush Spray the stainer bowl and exterior case using methanol or ethanol Wipe clean with a paper towel Initial completion of daily procedure on preventive maintenance PM chart 5 When ready to use the instrument again press CLEAN to reprime reagent lines Runa SPRAY PATTERN test to verify nozzle performance before staining Should any pattern appear abnormal repeat step 2 to clean nozzle orifice m WEEKLY 1 Perform SPRAY VOLUME test as described in Section 6 j Record the volume collected from each nozzle on PM chart 2 If volume trends lower or spray pattern is abnor
3. SS 041AA Gram Reagent A Decolorizer with Acetone and Safranine contains 70 to 80 Isopropyl Alcohol 20 to 30 Acetone lt 1 Safranine SS 041 AAF Gram Reagent A Decolorizer with Acetone and Fuchsin contains 70 to 80 Isopropyl Alcohol 20 to 30 Acetone 0 1 0 2 Basic Fuchsin SS 141A Gram Reagent A Safranine Concentrate when diluted with isopropanol acetone as directed contains 70 to 80 Isopropyl Alcohol 20 to 30 Acetone lt 1 Safranine SS 141A Gram Reagent A Safranine Concentrate contains 25 30 Deionized Water 5 10 Safranine SS 141 AF Gram Reagent A Fuchsin Concentrate when diluted with isopropanol acetone as directed contains 70 to 80 Isopropyl Alcohol 20 to 30 Acetone 0 1 0 2 Basic Fuchsin SS 141AF Gram Reagent A Fuchsin Concentrate contains 25 30 Deionized Water 2 4 Basic Fuchsin SS 041B Gram Reagent B Iodine contains 92 98 Deionized water lt 1 Iodine lt 1 Potassium Iodide SS 141B Gram Iodine Concentrate when diluted as directed contains 92 98 Deionized water lt 1 Iodine lt 1 Potassium Iodide SS 141B Gram Iodine Concentrate contains DOC 00309 ENA doc Page 15 of 27 Wescor Inc 60 70 Deionized Water 5 10 Potassium Iodide 2 5 5 Iodine SS 041C Gram Reagent C Crystal Violet contains 95 99 Deionized water 0 1 0 2 Crystal Violet SS 141C Gram Crystal Violet Concentrate when diluted as directed contains 95 99 Deionized water 0 1 0 2 Crystal Violet SS 141C Gram Rea
4. and the desired numbered keypad The stainer allows selecting decolorizer applications on a scale of 1 to 9 1 being light for thin smears and 9 being heavy decolorization suitable for thick specimen smears To change the decolorizer settings press PROG Press 1 for the decolorizer option Press the numbered keypad that corresponds with the desired cycle The setting selected remains current until changed or power is interrupted iv Stain Adjustments After finding the decolorizer setting that works for the desired application adjust the amount of crystal violet Reagent C and iodine Reagent B Both reagents have 3 settings The default setting for crystal violet and iodine is Medium which works well for most specimens To adjust the amount of crystal violet and or iodine applied to the slides press PROG to open the program menu Press 3 to select the Adjust Stain Menu Select either 1 for crystal violet or 2 for iodine Select 1 2 or 3 for Low Medium or High amounts of stain Stain adjustments are saved even if the decolorizer settings are changed v Load Carousel Press the center button to remove the carousel lid Load the 12 slide carousel with labeled end of the slide facing the outer rim of the carousel Load the 30 slide carousel with labeled end of the slide facing the carousel hub Smears in either carousel must face clockwise The stainer can be programmed to save reagent when staining partial loads in either the 12 slide or
5. be disposed of either by routing to an authorized local facility approved for handling hazardous materials or returning the equipment to Wescor Inc END DOC 00309 ENA doc Page 27 of 27 Wescor Inc
6. bottle in the box that corresponds to the reagent changed and description of the nature of problem and 3 any current months numeric date corrective action taken to resolve or correct problem 1 Check internal and exterior tubing and fittings for cracks leaks or any type of deterioration Replace as needed DOC 00309 ENA doc Page 22 of 27 Wescor Inc p Troubleshooting If you suspect a problem with reagent delivery diagnose the problem by assessing the performance of each spray nozzle Press the prime buttons and observe each nozzle for uniform dispersion pattern shape and direction of spray Test spray pattern and spray volume to determine which nozzle is not functioning properly Use the instructions under Disassembly and Cleaning to clean any nozzle that does not appear to function normally Disassembly and Cleaning The automatic clean cycle eliminates the evaporative accumulation of reagent solute in the nozzle system Foreign material may however get into the system and eventually plug the nozzle If this happens manually disassemble and clean the spray nozzle as follows DOC 00309 ENA doc 1 Slide the nozzle tool over the spray nozzle and turn counter clockwise to loosen and remove 2 While taking the nozzle apart become familiar with the nozzle its parts and assembly NOTE The following procedure requires using the Nozzle Maintenance Kit Use the kit to prevent mixing nozzles or nozzle parts When removing Aer
7. if instrument will be idle for more than 8 hours 1 Run a CLEAN cycle Leave instrument in CLEAN TO REPRIME mode 2 Use a spray bottle filled with methanol or ethanol and spray the front of each nozzle and clean each nozzle orifice with nozzle brush 3 Spray the stainer bowl and exterior case using methanol or ethanol Wipe clean with a paper towel 4 Initial completion of daily procedure on preventive maintenance PM chart 5 When you are ready to use the instrument again press CLEAN to reprime reagent lines Run a SPRAY PATTERN test to verify nozzle performance prior to staining Should any pattern appear abnormal repeat step 2 to clean nozzle orifice WEEKLY 1 Perform SPRAY VOLUME test Record volume collected from each nozzle on PM chart 2 If volume trends lower or spray pattern is abnormal disassemble and clean affected nozzle s Do not mix or interchange nozzles or nozzle parts Always return nozzles to same location in stainer Repeat SPRAY PATTERN and SPRAY VOLUME tests on cleaned nozzle s 3 Wipe down nozzles carousel tray and carousel lid using methanol or ethanol spray bottle or prepared SS 029C Nozzle Cleaning Solution with a paper towel 4 Slowly pour 200 300 mL of water into instrument drain to prevent build up of paper fibers precipitates etc Verify drain is flowing properly and not allowing fluid to back up in bowl or flow out of air vent on case back 5 Initial completion of weekly PM MONTHLY
8. 0309 ENA doc Page 10 of 27 Wescor Inc A list of Accessories follows A list of Supplies follows d In Vitro Diagnostic Use 8 7a and 8 4g Nozzle Cleaning Solution 355 mL SS 029 The symbol on the label recognizes the diagnostic use described above Intended purpose e Storage and Handling Conditions 8 7a and 8 4h Reagents are marked with a storage temperature range of 15 to 30 C degrees Do not freeze reagents or store in direct sunlight Temperatures slightly outside these specified limits for a short duration will not harm the reagents If the stainer will be idle for more than a week the following procedure prevents nozzle plugging problems when ready to use the instrument again Do this for each reagent line e Carefully loosen the ring cap from the reagent bottle e Lift the dip tube out of the bottle and wipe off any remaining reagent from the tube e Install a cap on the reagent bottle and set aside e Place the dip tube in a bottle of methanol or ethanol e Flush at least 250 mL of methanol or ethanol through the line and spray nozzle Leave methanol or ethanol in the line e Leave flushing fluids in the reagent lines during storage Do not run reagent lines dry e One at a time remove and disassemble each spray nozzle After removing o ring place metal nozzle parts in a 50 mL centrifuge tube provided in the Aerospray Maintenance Kit filled with methanol ethanol or prepared SS 029C DOC 00309 ENA d
9. E To prevent mixing nozzle parts use the tube stand inside the Nozzle Maintenance Kit Place tube containing nozzle parts in the punched hole that corresponds with the reagent line that the nozzle came from 12 Rinse parts in the tube with deionized water Continue rinsing until cleaning solution is completely removed from parts and tube 13 Rinse parts in the tube with methanol ethanol or prepared SS 029C then remove parts from tube 14 Reassemble nozzles and return nozzles to their original locations in the stainer Page 23 of 27 Wescor Inc o Nozzle Components Reassembly 1 Use a swab to apply a small amount of silicone lubricant SS 103 included with the instrument to the threads of the compression screw to prevent binding 2 Insert the swirl cone into the compression screw Hold this assembly and nozzle housing in a vertical position HOLD THIS POSITION UNTIL THE NOZZLE REASSEMBLY IS COMPLETED 3 Insert the long end of the hex wrench into the screw up to the stop Turn the compression screw into the nozzle housing Tighten firmly with the nozzle tool and hex wrench If assembled correctly the compression screw should screw into the nozzle housing approximately 1 4 inch 4 Replace the O ring 5 Install the spray nozzle by turning it in a clockwise direction Be sure to return each nozzle to its original position Use the nozzle tool and tighten to a snug fit DO NOT OVERTIGHTEN 6 Prime the nozzle and check s
10. WESTOR Document DOC 00309 EN Revision A Date Issued 2009 05 19 CO Number 09087 Essential Information for the Aerospray Gram Slide Stainer Cytocentrifuge Model 7320 its Accessories and Supplies DOC 00309 ENA doc Page 1 of 27 Wescor Inc Table of Contents 1 2 3 INTRODUCTION 00d aaaea aa a a aa aaa 3 SY1MBOESUSED S 2D asa aa 5 SYMBOLS USTD ON INSTRUMENTS AND REAGENTS A eN 5 HAZARDS 8 3 cilicioan 7 sR KANDA Bee i PT Ae rado dd dida asa 7 INTENDED PURPOSE 8 dis id 10 IDENTIFICATION LOT EXPIRATION DATES BS Ouija iaa 10 DETAILS FROM LABELS AND USER S MANUALS 8 7A AND 8 4A cssccccsssssssssssccssssssccssssssssssscsssssssssssssssssessees 10 Az MANUFACTURER NAME AND ADDRESS O A AND SA A A ass 10 B AUTHORIZED REPRESENTATIVE NAME AND ADDRESS 8 7A AND 8 4 A ococccncnnononononnnnnnnnnononoonnnnnnnnnnnnconcnnnnnnnnnn nono noncnnnnnnnnn rr nrnnnnnnnnoos 10 C IDENTIFICATION OF THE DEVICE ACCESSORY OR SUPPLY ITEM 8 7A AND 8 4B ccccssssscccccccceccceceesseeeeececeeceseeeesesseaseeeeeceeeeeeeaaes 10 Di INVITRO DIAGNOSTICO USES TA AND AO ias 11 E STORAGE AND HANDLING CONDITIONS O 7A AND SAA aaa 11 E OPERATING INSTRUCTION SAAND SA A adds 12 G APPROPRIATE WARNINGS AND PRECAUTIONS 8 7A AND 8 4J cccccssssssssnscccccecceccsssnaaeeeeeceececeecesseseesaaeescesesessesaaaaaaaeeceseceeeceseaeeaees 14 H COMPOSITION OF CRITICAL CHEMICALS IN REAGENT PRODUCTS 8 7B ccccsseeesessssnse
11. agent A Fuchsin Concentrate when diluted with isopropanol and acetone as directed are associated with the following Risk and Safety Phrases European Symbols of Danger are F and T S45 In case of accident or if you feel unwell seek medical advice immediately show the label where possible Avoid exposure obtain special instructions before use Reagents SS 041A Gram Reagent A Decolorizer with Safranin and SS 141A Gram Reagent A Safranin Concentrate when diluted with isopropanol and methanol as directed are associated with the following Risk and Safety Phrases European Symbols of danger are F and T Highly Flammable R 23 24123 Toxic by inhalation in contact with skin and 1f swallowed R 39 23 24 25 Toxic danger of very serious irreversible effects through inhalation in contact with skin and if swallowed SS 041AF Gram Reagent A Decolorizer with Fuchsin and SS 141 AF Gram Reagent A Fuchsin Concentrate when diluted with isopropanol and methanol as directed are associated with the following Risk and Safety Phrases European Symbols of danger are F and T Highly Flammable R 23 24 25 Toxic by inhalation in contact with skin and if swallowed R 39 23 24 25 Toxic danger of very serious irreversible effects through inhalation in contact with skin and if swallowed DOC 00309 ENA doc Page 7 of 27 Wescor Inc S 36 37 Wear suitable protective clothing and gloves In case of accident or if you feel unwell seek med
12. art for daily weekly and monthly maintenance is considered maximum Field experience suggests the more the Aerospray Slide Stainer is used the less frequently it requires preventive maintenance Do preventive maintenance at the level necessary to keep nozzle performance within specification and slides staining correctly New Spray Volume If nozzle was cleaned Page 21 of 27 Drain Line Flush see back page weekly step 4 Initial Warning Always wear eye and hand protection when performing preventive maintenance on the Aerospray Slide Stainer This Preventive Maintenance Chart may be reproduced in its entirety by Aerospray users Reagent A WESCOR INC 370 West 1700 South Logan Utah 84321 USA 435 752 6011 Previous Months Ending Volume Monthly Volume After Monthly Cleaning Disinfect DI Water Bottle Y when cleaned Not Applicable Not Applicable Not Applicable Not Applicable Annually or as needed Volume relationships between nozzles are as important as the actual volumes collected Investigation should center on whether the nozzles are delivering close to the same volumes Wescor Inc 800 453 2725 Fax 435 752 4127 email wescor wescor com Wescor Inc Clean Reagent Nozzles and Instrument Initial Check internal and exterior tubing and fittings Date tat Supervisor Approval Preventive Maintenance Procedure DAILY At end of each shift or
13. attern Test and which reagent line is being tested The instrument sprays a short burst of the corresponding reagent f The spray pattern must be round and uniform If the spray pattern is abnormal a clogged nozzle may be the reason Resolution to this problem is usually achieved by doing one or more of the following O AO TP Very Pattern Abnormal Pattern Abnormal Pattern Wipe the nozzle orifice with methanol or ethanol then run a CLEAN cycle Briskly push the bristles of the nozzle cleaning brush into the nozzle orifice Repeat several times to allow bristles to remove debris blocking the orifice 3 Disassemble and clean the nozzle NO g Press STOP to exit Pattern Test h Ifthe spray pattern is normal but staining results are abnormal perform a Slide Pattern Test Slide Pattern Test a Place a 26 mm x 76 mm 1 X 3 piece of paper in slots 1 and 2 of the carousel with a blocking slide in the slot before the paper slides Replace the carousel lid Load the carousel into the instrument and close the lid Press VOLUME TEST Press 1 Press the manual prime button for the reagent line to be tested This sprays the paper slide and reveals the pattern of stain application to the slide Remove the paper slides Repeat steps a to e for each reagent line Press STOP to exit the Pattern Test The pattern on the slide should be uniform without any continuous lines or streaks If continuous lines or streaks on t
14. ceecceccessssneaeeeeeececeeceeseseseessaeecceeceeseeeeesnaaeeeees 15 L STORAGE CONDITIONS AND S HE ELITE SAO ata AA A 17 Es PERFORMANCE OF DEVICES ID Aid 17 Ke SPECIAL EQUIPMENT REQUIREDES TES AAA AA AAA ES ita 18 ls YPELOP SPECIMEN TO BE USED CO IF heiir a OE onsets dead tacuisandaw esse AA N A ANA 18 Me PROCEDURE FOR GSE O TO Nocio ana tess SA Ia 19 Ne INTERNAL OA Y CON TROD 651K reri tods 19 O PERFORMANCE VERIFICATION MAINTENANCE AND SAFE WASTE DISPOSAL 8 7N ccccsscccceeeesseessssseaeeeeceseesessssnseceeeeececeeeeeeeeeeess 19 Pi TROUBLESHOOTING cis En EATE E A EEEE E EE OE E EA ES 23 O SETUPAND PRE USE PROCEDURES STO aia 24 R PROCESS FOR CLEANING DECONTAMINATION 8 TQ ii ii tt a ti 26 S ENVIRONMENTAL SPECIFICATIONS AND PRECAUTIONS 8 7R ccsssssssscccccecsecccesesssseeececcceecesseeesessaaaaeceeessessesaaaeaeeeeeeeseeeceeseeeeeseceeeceeses 2 E DISPOSAGOF DEVICES Sa ia ee 2 DOC 00309 ENA doc Page 2 of 27 Wescor Inc 1 Introduction This document contains the information required by the In Vitro Diagnostic Directive 98 79 EC Annex I Essential Requirements Part B Section 8 Information supplied by the manufacturer for the Aerospray Gram Slide Stainer Cytocentrifuge Model 7320 its accessories and supplies In particular it describes any symbols used on labels and on the instrument hazards associated with the stain reagents used the intended purpose of the device lot numbers and expiration dates a
15. e and its reagents Rear Panel Label M EXHAUST VENT U S PATENT NUMBER 5 009 185 OTHER FOREIGN PATENTS APPLY INPUT 100 240 V7 50 60 Hz 85 VA E T2A250V EC REP MT Promedt Consulting GmbH Altenhofstrasse 80 D 66386 St Ingbert GERMANY ME Aerospray 7320 User s Manual INACCORDANCE WITH UL 61010 1 IEC EN 61010 1 CAN CSA C22 2 No 61010 1 04 Model Serial Number Label Aerospray Gram Slide Stainer Cytocentrifuge Model 7320 Side Panel Membrane Switch Label oN mn l Made in United States Wescor Inc 370 West 1700 South Logan UT 84321 USA Dx B C D AN CAUTION REFER TO USER S MANUAL FOR REPLENISHMENT PROCEDURE DOC 00309 ENA doc Page 14 of 27 Wescor Inc h Composition of Critical Chemicals in Reagent Products 8 7b SS 041A Gram Reagent A Decolorizer with Safranine contains 55 65 Isopropyl Alcohol 35 45 Methyl Alcohol lt 1 Safranine SS 041AF Gram Reagent A Decolorizer with Fuchsin contains 55 65 Isopropyl Alcohol 35 45 Methyl Alcohol 0 1 0 2 Basic Fuchsin SS 141A Gram Reagent A Safranine Concentrate when diluted with isopropanol methanol as directed contains 55 65 Isopropyl Alcohol 35 45 Methyl Alcohol lt 1 Safranine SS 141AF Gram Reagent A Fuchsin Concentrate when diluted with isopropanol methanol as directed contains 55 65 Isopropyl Alcohol 35 45 Methyl Alcohol 0 1 0 2 Basic Fuchsin
16. e label where possible Reagents SS 141C Gram Reagent C Crystal Violet Concentrate is associated with the following Risk and Safety Phrases European Symbols of Danger are T and N R 20 21 22 Harmful by inhalation in contact with skin and if swallowed R 68 20 2 1 22 Harmful possible risk of irreversible effects through inhalation in contact with skin and if swallowed May cause sensitization by skin contact Toxic to aquatic organisms May cause long term adverse effects in the aquatic environment Keep container tightly closed S 36 37 Wear suitable protective clothing and gloves Avoid exposure obtain special instructions before use In case of accident or if you feel unwell seek medical advice immediately show the label where possible SS 029 Aerospray Nozzle Cleaning Solution and SS 029C when diluted as instructed are associated with the following Risk and Safety Phrases European Symbols of Danger are F and T Keep away from sources of ignition No smoking DOC 00309 ENA doc Page 8 of 27 Wescor Inc R11 Highly Flammable R 23 24 25 Toxic by inhalation in contact with skin and if swallowed R 39 23 24 25 Toxic danger of very serious irreversible effects through inhalation in contact with skin and if swallowed S 16 S 36 37 S 45 SS 029C Aerospray Nozzle Cleaning Solution is associated with the following Risk and Safety Phrases European Symbol of Danger 1s None None None SS 230 Aerospra
17. es Five reagent dip tubes A through E connect the reagents to the internal pumps and spray nozzles Manual Priming Buttons These buttons operate the corresponding pumps for priming DOC 00309 ENA doc Page 3 of 27 Wescor Inc Aerospray Gram Slide Stainer Cytocentrifuge Rear Panel 7 Exhaust Vent 0 During operation air is drawn through og openings at the rear of the instrument lid and expelled through the exhaust vent Power Switch The main power switch turns the stainer On 1 and Off O When the instrument is connected to the proper power source and the power switch is on the front panel power indicator is lit Fuse Door To get to the main fuses turn the power off disconnect the power cord and use a small screwdriver to open the fuse door Follow normal safety precautions Drain Port Provides connection for 5 16 inch inside diameter drain tube AC 041 This port must be connected to a drain or vented waste container Volume Control Adjusts the volume of the signal tone sounded when a staining or cytocentrifuge cycle is complete Turn clockwise to increase volume Power Entry Module Provides connection for a standard IEC 320 type power cord provided DOC 00309 ENA doc Page 4 of 27 Wescor Inc 2 Symbols Used 8 2 Symbols used on instruments and reagents Alternating Current AC Ec ner Authorized Representative in the European Community Batch Code AS Bio
18. es run a staining cycle by pressing RUN to start the cycle During the cycle the display shows the current decolorizer setting and reagent currently being applied At the bottom of the display an increasing bar graph and percentage complete symbol indicate the approximate time remaining in the cycle When the cycle is complete the signal tone sounds ix Clean Cycle X DOC 00309 ENA doc CAUTION Remove all specimen slides before using the clean or reprime cycles otherwise specimens will be ruined At the end of each shift or whenever the stainer will be idle for more than four hours use the CLEAN cycle to maintain nozzle performance Place an empty carousel in the stainer and close the lid Press CLEAN Leave the stainer with Press Clean to Reprime on the display while it is idle When ready to stain slides make sure there is a carousel in the stainer and press CLEAN to reprime the stainer After running the CLEAN cycle spray nozzle faces with methanol ethanol or prepared Nozzle Cleaning Solution SS 029C Use a nozzle brush to clean the nozzle orifices when patterns are less than optimal Emergency Stop The STOP button interrupts any cycle immediately Pressing STOP during a clean or reprime cycle establishes a warning routine that cannot be escaped until a clean and reprime cycle is repeated Page 13 of 27 Wescor Inc g Appropriate Warnings and Precautions 8 7a and 8 4j Following are warning labels for the devic
19. gent C Crystal Violet Concentrate contains 45 55 Deionized Water lt 5 Crystal Violet SS MeOH Reagent Grade Methanol contains gt 99 5 Methyl Alcohol Reagent Grade Anhydrous SS 029 Nozzle Cleaning Solution contains 40 50 Methyl Alcohol 1 5 Oxalic Acid SS 029C SS 029CG Nozzle Cleaning Solution Concentrate 95 99 Deionized Water 1 5 Oxalic Acid SS 230 Aerospray Stain Residue Solvent contains 70 85 Dimethyl sulfoxide SS 133 Decontamination Solution Concentrate contains lt 30 Germicidal Detergent gt 70 Deionized Water SS 133 Decontamination Solution when diluted as directed contains lt 2 Germicidal Detergent gt 98 Deionized Water DOC 00309 ENA doc Page 16 of 27 Wescor Inc i Storage Conditions and Shelf Life 8 7c Reagents should be stored at room temperature away from direct light Expiration dates are shown on each reagent label with the international symbol gg j Performance of Device 8 7d This is not a measurement instrument however running a spray pattern and volume test can check instrument performance Run this test if any abnormal results are observed after completing a staining cycle DOC 00309 ENA doc Spray Pattern Test Remove the carousel from the instrument Press VOLUME TEST Press for Pattern Test Hold a sheet of white paper in front of the carousel hub Press the prime button that corresponds with the reagent line to be tested The display shows the selected P
20. he paper slides are observed disassemble and clean the defective nozzle ao gs Sua rh o Spray Volume Test a To test the volume of stain being delivered press VOLUME TEST Press 2 to select Volume Test c Hold a small container such as the 14 mL centrifuge tube included with the Aerospray Maintenance Kit to capture the spray from the desired nozzle Press the corresponding prime button The pump for that position runs for 20 seconds e Place the centrifuge tube with the collected reagent in the tub stand of the maintenance kit Place the tube in the position that corresponds to the reagent line being tested Use the information found in Interpreting Results to determine the results of the Spray Volume test f To exit Volume Test press STOP Page 17 of 27 Wescor Inc iv Interpreting Results When factory new the B C D and E nozzles should deliver 9 0 to 11 0 mL over the 20 second spray sequence The A nozzle should deliver between 10 and 12 mL Older nozzles may exhibit higher volumes The instrument stains properly with reagent deliveries above and below these levels Volume relationships between nozzles are at least as important as the actual volumes collected and your investigation should center on whether these nozzle groups are delivering close to the same levels When operating correctly nozzles B C and D front should deliver approximately the same volume of stain The D rear nozzle volume may be slightly highe
21. ical advice immediately show label where possible Avoid exposure obtain special instructions before use Reagents SS 041B Gram Reagent B Iodine and SS 141B Gram Iodine Concentrate when diluted as directed are associated with the following Risk and Safety Phrases European Symbols of Danger are None Reagents SS 041C Gram Reagent C Crystal Violet and SS 141C Gram Crystal Violet Concentrate when diluted as directed are associated with the following Risk and Safety Phrases European Symbols of Danger are T S 36 37 Wear suitable protective clothing and gloves In case of accident or if you feel unwell seek medical advice immediately show label where possible Avoid exposure obtain special instructions before use Reagents SS 141B Gram Iodine Concentrate is associated with the following Risk and Safety Phrases European Symbols of Danger are None Reagents SS 141A Gram Reagent A Safranine Concentrate is associated with the following Risk and Safety Phrases European Symbol of Danger is X1 In case of contact with eyes rinse immediately with plenty of water and seek medical advice Reagents SS 141AF Gram Reagent A Fuchsin Concentrate 1s associated with the following Risk and Safety Phrases European Symbol of Danger is T S 45 In case of accident or if you feel unwell seek medical advice immediately show the label where possible S 53 In case of accident or if you feel unwell seek medical advice immediately show th
22. lide Stainer Cytocentrifuge is intended for use by medical professionals to stain specimens that typically include microbes for identification of Gram Positive or Gram Negative Characteristics as a step of standard laboratory practice in diagnosing disease in humans Addition of the Cytopro rotor allows preparation of slides by cytocentrifugation before staining 5 Identification Lot Expiration Dates 8 6 The device and all accessories and supplies are clearly identified Labels on reagents include lot number and expiration date information as shown in Section 6 6 Details from Labels and User s Manuals 8 7a and 8 4a a Manufacturer Name and Address 8 7a and 8 4a Wescor Inc 370 West 1700 South Logan UT 84321 8212 USA Phone 1 435 752 6011 Fax 1 435 752 4127 Email service wescor com www wescor com b Authorized Representative Name and Address 8 7a and 8 4a Medical Technology Promedt Consulting Altenhofstrafe 80 66386 St Ingbert Germany Tel 49 6894 581020 Fax 49 6894 581021 Email info mt procons com c Identification of the Device Accessory or Supply Item 8 7a and 8 4b The model number and name is on the front panel of the instrument Accessories and supplies are labeled with names and product numbers Aerospray Gram Slide Stainer Cytocentrifuge Faceplate Label Print 0003 AEROSPRAY CLEAN VOLUME TEST ROTATION POWER 7320 GRAM SLIDE STAINER CYTOCENTRIFUGE DOC 0
23. logical Hazards Biological Risks Catalog Number Model Number Caution Consult Accompanying Documents Attention see instructions for use Dior MY Joanna CIA VD In vitro Diagnostic Device In vitro Diagnostic Medical Device Me Keep away from sunlight Keep away from heat i moe rE DOC 00309 ENA doc Page 5 of 27 Wescor Inc Off Power o on Power o0 RoHS Pollution Control Serial Number Temperature Limitation indicates high and low limits normal room temperatures are specified for all Wescor Reagents Use By Volume Control General Warning Caution Risk of Danger Warning Biological Hazard Corrosive Environment Hazard Flammable Harmful Irritant Oxidant Toxic Product to be used for manual cleaning only Do pump product through instrument DOC 00309 ENA doc Page 6 of 27 Wescor Inc 3 Hazards 8 3 a Risk and Safety Phrases Reagents SS 041AA Gram Reagent A Decolorizer with Acetone and Safranine and SS 141A Gram Reagent A Safranin Concentrate when diluted with isopropanol and acetone as directed are associated with the following Risk and Safety Phrases European Symbols of Danger are F and X1 Keep away from sources of ignition No smoking S 24 25 Avoid contact with skin and eyes In case of contact with eyes rinse immediately with plenty of water and seek medical advice SS 041 AAF Gram Reagent A Decolorizer with Acetone and Fuchsin and SS 141 AF Gram Re
24. mal disassemble and clean affected nozzle s Do not mix or interchange nozzles or nozzle parts Always return nozzles to same location in stainer Repeat SPRAY PATTERN and SPRAY VOLUME tests on cleaned nozzle s 3 Wipe down nozzles carousel tray and carousel lid using methanol or ethanol spray bottle or prepared SS 029C Nozzle Cleaning Solution with a paper towel 4 Slowly pour 200 300 mL of water into instrument drain to prevent build up of paper fibers precipitates etc Verify drain is flowing properly and not allowing fluid to back up in bowl or flow out of air vent on case back 5 Initial completion of weekly PM MONTHLY DOC 00309 ENA doc Page 19 of 27 Wescor Inc Disassemble and clean all nozzles as described in the following section Section 6 p Do not mix or interchange nozzles or nozzle parts 2 Remove Pick up Tube from Reagent B Iodine bottle Then A Flush 500 mL DI water through the line B Flush 100 mL prepared SS 029C through the line Block flow at nozzle holder with a gloved finger for a few seconds during flushing Stop flushing just before 100 mL is gone Soak in line 1 hour to overnight as practical C Flush prepared SS 029C out of line with 500 mL of DI water D Reinstall Reagent B Iodine and flush 100 mL through the line to remove DI water 3 Reinstall nozzles Always return nozzles to the same location in stainer 4 Perform SPRAY PATTERN and SPRAY VOLUME tests Record results from end
25. nal sputum bronchial washes Medium Smears CSF urine wounds etc 5 Medium and Sputum bronchial washes wounds 6 Thick Smears blood cultures etc 7a Thick Smears Sputum bronchial washes tissues blood cultures etc 8 Very thick smears stools blood 9 cultures etc Over decolorization and Weak Control Cultures It is well known that weakly gram positive cultures such as Bacillus sp and Streptococcus sp can be easily over decolorized especially when cultures are old or stressed When decolorizing such cultures by hand de colorization can be limited to just a few seconds as the process can be judged by eye Such cultures tend to be over decolorized in the Aerospray unless the decolorizer application 1s limited by running at setting 1 or 2 A strongly positive culture such as 18 DOC 00309 ENA doc Page 18 of 27 Wescor Inc hour Staphylococcus should remain mostly positive even at decolorizer setting 9 In addition samples at the extreme edges of the slide may under decolorize or even over decolorize depending on the state of the nozzle spray pattern For the best results use the center half of the slide for specimen loading Fouled nozzles usually cause over decolorization of normal specimens in the Aerospray If over decolorization problems persist even though nozzle performance is within specifications and the iodine and other reagents are not expired contact an authorized representative for assistance
26. nc 6 With the stainer lid closed apply decontamination solution to a cloth Wipe the exterior surfaces of the stainer Do not flood the display panel with excessive moisture Any moisture that seeps through could damage the internal electronics Repeat wipe down every 2 or 3 minutes for about 10 minutes total 7 Remove decontamination solution by thoroughly wiping surfaces with a cloth soaked in tap water 8 Immerse or generously spray the carousel and lid with disinfectant detergent Allow to sit for 20 to 30 minutes Do not autoclave the carousel or lid 9 Thoroughly rinse the carousel and lid with tap water NOTE These decontamination procedures are for routine use only If shipping the stainer to Wescor for repair or service contact Wescor s Service department for a current copy of the decontamination and shipping instructions before preparing and shipping the stainer Shipping stainers without decontaminating them according to these instructions is dangerous to service personnel and results in a significant decontamination charge s Environmental Specifications and Precautions 8 7r Device conforms to EMC directive 89 336 EC Using this instrument in a manner not specified by Wescor may impair the safety protection designed into the equipment and may lead to injury t Disposal of Device 8 7s The device is subject to the WEEE Directive 2002 96 EC and cannot be disposed of in a normal landfill Instead the equipment must
27. nd instructions for the use and maintenance of the device Some sub requirements of Section 8 are not applicable to this product but the applicable requirements are referenced herein This document is available in the official language of each EC member state where the product is sold that requires information in its own language Additional helpful information may be found in Wescor User s Manuals Service Manuals Technical Bulletins or other information supplied by Wescor or its Authorized Distributors for specific countries Some supplementary material is in the English language only Many of these materials may be found on Wescor s web site www wescor com A Document Packet 1s included with each Aerospray Gram Slide Stainer Cytocentrifuge which includes MSDS sheets a Declaration of Conformity Nozzle Cleaning Instructions an Installation Checklist and a User s Manual 8 1 The cytocentrifugation function is available with the addition of the Cytopro rotor and is described in a separate Essential Information document and manual Aerospray Gram Slide Stainer Cytocentrifuge Front and Side Slide Staining Carousels Hold 1 to 12 or 1 to 30 slides The carousel mounts on the drive hub rotating at approximately 20 rpm for staining and approximately 950 rpm for drying Reagent Spray Nozzle Each reagent has a separate spray nozzle s to dispense the correct amount of reagent Reagent Bottle Dip Tub
28. nder normal use but cannot be considered decontaminated The stainer exterior can also be contaminated by touching with contaminated gloves and requires routine surface cleaning For additional decontamination the following procedures provide low to intermediate disinfection If the stainer is contaminated by unusually hazardous or disinfection resistant organisms further treatment with appropriate procedures may be necessary If sending the stainer to Wescor for service or repair contact Wescor for current decontamination and shipping instructions 1 Mask the lid latch and locking pin holes with waterproof tape to protect the stainer Interior Place the stainer in a biological safety hood or well ventilated area Use prudent safety precautions including hand and eye protection CAUTION Do not flood the stainer bowl by overloading the drain Never allow fluid to rise above the base of the drive hub Do not spray fluids near openings in the stainer housing that lets fluid into the instrument interior This can cause severe damage 3 Spray inner bowl and inner lid with disinfectant detergent such as a 10 bleach solution or Wescor s Decontamination Solution SS 133 4 Repeat spray treatment every 2 or 3 minutes Leave solution on surfaces for approximately 10 minutes Do not allow cleaning solutions to dry on the stainer surfaces gt Rinse inner bowl and lid thoroughly with tap water DOC 00309 ENA doc Page 26 of 27 Wescor I
29. oc Page 11 of 27 Wescor Inc e Mark the tube to identify the correct reagent line Use the provided cleaning kit tube stand to store the tubes Place each tube holding nozzle parts in the position corresponding with the reagent line that the nozzle is removed from Be sure the nozzles are returned to their original position in the stainer e Flush the instrument drain tube with water to prevent build up of paper fibers precipitates or other materials CAUTION Do not subject stainer to freezing temperatures while aqueous fluids remain in any reagent lines Serious damage can result f Operating Instruction 8 7a and 8 4i i Initial Setup e Install the drain tube e Plug in the power cord and switch the power on e Install all reagent bottles e Prime all reagent lines e Run the CLEAN cycle twice to purge the reagent nozzles of precipitates and debris See Clean Cycle 6 f 1x NOTE Dirty nozzles cause most staining problems Check spray pattern and clean nozzles as necessary Select Alcohol Fixation To activate or deactivate the alcohol fixation function press PROG to open the program menu Press 2 to select the alcohol fixation menu Select 0 for Off 1 for Normal or 2 to turn alcohol fixation to High The display indicates the status of the alcohol fixation Deactivate the alcohol fixation function if hand fixing slides iii Selecting Decolorizer Cycle Change the decolorizer intensity as needed by pressing the PROG keypad
30. of month SPRAY VOLUME test on preventive maintenance chart NOTE When entering a new month the Volume After Monthly Cleaning number becomes Previous Months Ending Volume number 5 Disinfect reusable bottles with a 1 10 dilution of bleach Rinse thoroughly with deionized water 6 Initial completion of monthly PM 7 Supervisor check and initial ANNUALLY 1 Check internal and exterior tubing and fittings for cracks leaks or any type of deterioration Replace as needed DOC 00309 ENA doc Page 20 of 27 Wescor Inc Preventive Maintenance Chart Month Year Daily Clean Cycle and Wiping Daily Control Reagent 1 2 3 4 D Front 5 D Back 6 T 8 9 10 D Front 11 D Back 12 13 14 15 16 D Front 17 D Back 18 19 20 21 22 D Front 23 D Back at gt E optional 31 DOC 00309 ENA doc Spray Pattern Y if okay IR WESC Aerospray Gram Slide Stainer Cytocentrifuge Model 7320 Weekly Nozzle Performance Expected Spray Volume 10 12 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 10 12 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 10 12 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 10 12 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL 9 0 11 0 mL Measured Spray Volume Preventative Maintenance Chart procedures are located on reverse side The frequency set forth on this ch
31. ospray nozzle s be sure to return all nozzles to their original positions after cleaning This helps ensure consistent staining performance 3 Hold the spray nozzle with the nozzle tool and insert the 5 32 nozzle hex wrench into the compression screw Turn counter clockwise to loosen and remove DO NOT DROP COMPRESSION SCREW OR SWIRL CONE 4 Remove the o ring NOTE Excessive force used to loosen and remove the compression screw can damage the plastic nozzle tool If the compression screw cannot be readily loosened soak the nozzle in methanol ethanol or prepared SS 029C to remove residue If the problem continues use light penetrating oil and a 5 8 inch wrench to loosen the nozzle CAUTION Do not use hardened metal instruments to clean or scrape nozzle components 5 Place disassembled metal nozzle parts in one of the provided 50 mL centrifuge tubes 6 Fill tube to 25 mL mark with methanol ethanol or prepared SS 029C 7 Cap tube agitate and then soak parts until clean WARNING Always wear protective clothing and eye protection when using the SS 029C Dispose of used cleaning solution in the correct manner 8 Agitate again and pour off the solution 9 Use the provided cleaning wire AC 059 to clean the nozzle orifice 10 Remove any material in the swirl cone grooves by sliding the edge of a piece of paper through each groove 11 Inspect nozzle parts to ensure they are completely clean Repeat soaking if necessary NOT
32. ottles front to back in the following order A Decolorizer with Counterstain B Iodine C Crystal Violet D Deionized Water E Anhydrous Methanol or Ethanol s 0 5 water DOC 00309 ENA doc Page 24 of 27 Wescor Inc WARNING Reagents used in the Aerospray Stainer contain moderately hazardous chemicals that require care in handling Always use appropriate safety measures including gloves and eye protection when handling reagents CAUTION To avoid severe damage never use reagents containing organic solvents in this instrument unless they are supplied by Wescor or specified in official Wescor formulation instructions Remove the cap from each bottle Remove the central portion of the seal Insert the dip tubes into the reagent bottles and screw on the ring caps iv Aerospray Nozzle Maintenance Kit The Aerospray Nozzle Maintenance Kit AC 075 is provided to help maintain optimum performance from the reagent delivery system The kit contains tools and equipment to clean the nozzles check reagent delivery and to prime the instrument v Prime all reagent lines For peak stainer performance thoroughly purge and prime each reagent delivery line using the following instructions This procedure uses approximately 250 mL of reagent per line Extra reagent is included with the new instrument for this purpose Remove each spray nozzle with the provided nozzle tool by turning it counterclockwise Note the location of each nozzle so
33. pray pattern and spray volume before staining I Safe Waste Disposal Disposal of fluids may be governed by local regulations consult material safety data sheets q Setup and Pre use Procedures 8 70 I Installing Drain Tube Place the slide stainer on a level counter surface near a sink or suitable drain or waste container Attach the drain tube to the drain port on the back lower right of the unit Route the tube to a drain or vented waste container Make certain the tube remains below the drain port Run the tube continuously downward to the drain or waste container so that liquid cannot be trapped in the line Keep the tube as short as possible no longer than 1 8 meters NOTE Do not submerge the end of the drain tube in liquid this interferes with draining li Connecting Power 1 The power switch is located on the back panel in the power entry module 2 Make certain the switch is OFF O 3 Plug the female end of the power cord into the power entry module 85 to 264 Volts AC NOTE We recommend utilization of a power line surge protector to isolate the instrument from spikes and surges 4 Plug the male end of the power cord into a grounded power outlet 5 Turn the power switch ON I The power indicator on the front panel should now be on The display initially shows the software version NOTE Leave the power on except when the instrument is serviced or moved iii Install all reagent bottles Place the reagent b
34. r or lower Reagent A will almost always be higher Reagent E can be higher or lower than the other nozzles except Reagent A Low Volume Low volume from a spray nozzle is typically caused by reagent precipitate or foreign matter inside the nozzle If the CLEAN cycle does not help the best solution is to manually disassemble and clean the spray nozzle Excessive Volume If collecting excessive volume make sure the nozzle is assembled correctly see Reassembly in Section 60 If this fails to correct the problem contact an authorized representative or Wescor for assistance k Special Equipment Required 8 7e See the list of Accessories in Section 6c for the maintenance tools and supplies provided with each stainer Type of Specimen to be Used 8 7f The Aerospray Gram Slide Stainer Cytocentrifuge stains specimens applied to microscope slides Nine decolorizer settings allow compensation for slide specimen variation The chart below suggests recommended settings for various specimens All specimens should be as thin as possible If prepared as thin smears most specimens stain satisfactorily on settings 3 or 4 NOTE These are suggested settings only Each laboratory should establish its own protocol for gram staining DECOLORIZER THICKNESS SUGGESTED SETTING SPECIMEN 1 Very Thin Smears Weak reagent s instrument problem old culture s Y Thin Smears CSF urine peritoneal vag wounds 3 bronchial washes etc 4 Thin and Vagi
35. sequence above Use a carousel in the instrument A fine cone of spray should come from each nozzle Run the Spray Volume and Spray Pattern tests After verifying nozzle performance run the CLEAN cycle vi Run the CLEAN cycle The CLEAN cycle is a two stage process that uses methanol or ethanol to purge the reagent nozzles of precipitates and debris then reprimes reagents into the nozzles Each stage of the cycle requires user input to continue the DOC 00309 ENA doc Page 25 of 27 Wescor Inc r process This allows delaying the second stage indefinitely The steps described below are for initial setup see following Note routine cleaning of the instrument or to keep the nozzles clean while the instrument is idle We recommend frequent use at least once per shift of the CLEAN cycle to ensure reliable nozzle performance NOTE Run the following CLEAN cycle routine twice during initial setup to help remove any air bubbles from the reagent lines and ready the instrument for slide staining e Place an empty carousel in the instrument and close the lid e Push CLEAN This sprays methanol or ethanol Reagent E through the nozzles and onto the carousel When the cycle is completed the display reads PRESS CLEAN TO REPRIME e Wipe nozzle orifices to remove all residual liquid e At this point the instrument stands by with methanol or ethanol remaining in the nozzles until pressing CLEAN again The instrument can be left in this
36. standby mode for extended periods to prevent clogging while the stainer is idle e Ifnot staining slides immediately leave the instrument in standby mode until ready to stain This is particularly important if the instrument will be idle for more than 4 hours Later when ready to stain slides make sure there is a carousel in the instrument then e Press CLEAN again This reprimes reagent into the nozzles making the instrument ready for staining CAUTION Do not place any carousel loaded with specimens in the instrument while CLEAN TO REPRIME appears on the display Specimens will be damaged if they contact excess reagents sprayed from the nozzles when you press CLEAN NOTE Pressing STOP during the clean or reprime cycles causes a warning to be displayed until a complete Clean and Reprime cycle is completed Process for Cleaning Decontamination 8 7q Under normal clinical use the Aerospray Gram Slide Stainer Cytocentrifuge poses very little risk of biological infection to laboratory workers The stainer is essentially an environmental surface which should be kept clean Only low level disinfection is required Biological contamination occurs only when specimens slough off the slides during staining These tend to be removed by the continual flow of reagents through the instrument In addition the reagents used in the staining and cleaning process are suitable for low to intermediate level disinfection The stainer is self cleaning u
37. the 30 slide carousel To use this feature place slides in the marked slots on the 12 slide or 30 slide carousel and enter the number of slides on the keypad Put the first slide in position 1 the second in position 2 and so on If there is an odd number of slides place a blank slide in the next position for balance If there are empty slots in the carousel place a blank blocking slide in the first empty slot clockwise from a specimen slide and another blank blocking slide directly across the carousel vi Load Instrument Replace the carousel lid by pressing the release button while lowering the lid over the indexing posts Release the button and press the lid handle lightly until it clicks into place Place the carousel into the stainer and close the stainer lid DOC 00309 ENA doc Page 12 of 27 Wescor Inc vii Program Number of Slides If staining a partial load enter the number of slides into the stainer by using the numbered keypads Slide selection defaults to Full Carousel at the end of the run or after pressing STOP This instrument treats anything over a partial load as a full carousel To stain more than a partial load there is no need to program the number of slides A partial load on the 12 slide carousel is anything from 1 to 6 slides A partial load on the 30 slide carousel is anything from 1 16 slides viii Running a Staining Cycle After programming the decolorizer setting fixation stain adjustments and number of slid
38. y Stain Residue Solvent is associated with the following Risk and Safety Phrases European Symbols of Danger are None one one SS 133 Decontamination Solution Concentrate is associated with the following Risk and Safety Phrases European Symbols of Danger is C R 34 R 22 R 67 S 24 25 S 26 In case of contact with eyes rinse immediately with plenty of water and seek medical advice S 36 37 39 Wear suitable protective clothing gloves and eye face protection S 45 In case of accident or if you feel unwell seek medical advice immediately show the label where possible SS MeOH Aerospray Reagent Grade Methanol is associated with the following Risk and Safety Phrases European Symbols of Danger are F and T R11 Highly Flammable R 23 24 25 Toxic by inhalation in contact with skin and if swallowed R 39 23 24 25 Toxic danger of very serious irreversible effects through inhalation in contact with skin and if swallowed Keep container tightly closed S 16 Keep away from sources of ignition No smoking S 36 37 Wear suitable protective clothing and gloves S 45 In case of accident or if you feel unwell seek medical advice immediately show label where possible SS 103 O ring Nozzle Thread Grease is associated with the following Risk and Safety Phrases European Symbols of Danger are None None None DOC 00309 ENA doc Page 9 of 27 Wescor Inc 4 Intended Purpose 8 5 The Aerospray Gram S
39. you can return it to the original location Place a carousel in the stainer to prevent stain from entering the motor shaft Briefly press each prime button Stain should appear within 10 seconds If no stain appears within 10 seconds there may be an air lock in the line Immediately stop priming Use Reagent Pump Priming Tool AC 069 part of Nozzle Maintenance Kit to remove the air lock If stain begins to appear within 10 seconds or once an air lock 1s resolved prime all reagent lines This can be done manually or by using Prime Lines or Volume Test functions When properly primed a steady stream of reagent no sputtering or breaks flows from each nozzle receptacle while pressing the corresponding prime button WARNING Never operate a dry pump longer than 10 seconds Simultaneously Priming All Lines Press VOLUME TEST Press 4 on the keypad to prime all the reagent lines The pumps run for 60 seconds After priming reagent lines replace the nozzles Return each nozzle to its original location for consistent staining performance Priming Individual Lines Prime manually for 60 seconds or press VOLUME TEST Press 2 on the Keypad Press the desired prime button The pump for that position runs for 20 seconds Repeat twice more to achieve 60 seconds of flow After priming all reagent lines replace the nozzles Return each nozzle to its original location for consistent staining performance With the nozzles installed repeat the priming
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