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8 x 50mL Individually Sealed Rotor for General

1. 1 c C Solution 555 20 Magnesium Chloride Mercaptoacetic Acid Methyl Alcohol Methylene Chloride Thermo Scientific co co c co lt C anaj C BUNAN co lt CO lt cC zZ lt c C CO CO CO co co C DELRIN Mm DA nm CO lt nm co Mm nm G wm lt lt co zal co cO wm Z POLYALOMER co c a lt c c C c cO WM aj WM GW lt POLYCARBONATE co o wm lt c lt cC WM cc CO cO co a GW cO cO POLYPROPYLENE G co ajl nl o nl 75003694 8 50 sealed C CO CO C lt C lt C C YM lt C NM YM C C TITANIUM lt B chemical Compatibility Chart lt PET POLYCLEAR 1 2 5 5 a Methyl Ethyl Ketone 55 UUS S MS 5 UU 5
2. 2009 Thermo Fisher Scientific Inc All rights reserved AutoLoc is either a registered trademark or a trademark of Thermo Fisher Scientific All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history 50121058 3 p
3. Start loading the rotor on the outer ring If you only centrifuge one sample place a test tube with water in the opposite position to balance the load Improper Loading 4 2 75003694 8x50 sealed Thermo Scientific 4 Rotor loading Maximum Loading Maximum Loading The rotor can run at high speeds The rotor design has sufficient reserve stability even when spinning at top speed The safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adaptor exceeds the maximum permissible load Reduce the fill level Reduce the speed Use the table or formula below Actual Load g RPM 189 6 700 199 6529 209 6371 219 6224 229 6087 239 5958 249 5837 259 5723 269 5616 279 5514 289 5418 299 5327 319 5157 Recalculate the maximum permissible speed according to the following formula and select the calculated maximum speed for the centrifuge _ Maximum permissible load 7 max Effective load admissible speed na Maximum speed Cycle Counter The lifetime of rotors is dependent on the amount of mechanical load Do not exceed the number of cycles recommended for rotors and buckets Thermo Scientific 75003694 8x50 sealed 4 3 4 Rotor loading Cycle Counter The maximum number of cycles is given in Rotor Specifications on page 1 1 WARNING
4. 60Hz ventilated 1 3 Heraeus 230 50Hz 60Hz refrigerated 1 2 Heraeus 230V 50Hz 60Hz ventilated 1 2 Improper loading 4 2 M Maintenance 6 1 Thermo Scientific Maximum loading 4 3 P Placing Oll aee pire te bend 5 2 Precautions nq io Esn iii Diele oun eio un Heu UD iii Proper loading aaa aan 4 2 0 EN 5 3 R RG eiue cda Odd A 1 Removing the rotor 3 3 Rotor installation 3 2 5 Scope f supply ili Service technician 6 5 Sorvall 100V 50 60Hz refrigerated 1 6 Sorvall 100V 50 60Hz ventilated 1 6 Sorvall 120V 60Hz refrigerated 1 5 Sorvall 120V 60Hz ventilated 1 5 Sorvall 230V 50Hz 60Hz refrigerated 1 4 Sorvall 230V 50Hz 60Hz ventilated 1 4 T Thermo Scientific 120V 60Hz refrigerated 1 8 Thermo Scientific 120V 60Hz ventilated 1 8 Thermo Scientific 230V 50Hz 60Hz refrigerated 1 7 Thermo Scientific 230V 50Hz 60Hz ventilated 75003694 8x50 sealed 1 1 Part of Thermo Fisher Scientific SCIENTIFIC
5. Portor down UK Table 1 10 Sorvall 100V 50 60Hz refrigerated Centrifuge Sorvall Legend X1R Sorvall ST 16R Catalog 75004263 75004383 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8 189 8 189 maximum speed Nmax rpm 6 500 6 500 maximum value at nmax 4 997 4 997 min radius 143 B 9 14 3 B 9 Pitch angle 45 45 Accel braking time s 40 35 40 35 Aerosol tight yes yes permissible temperature range 121 121 autoclavable C ested by HPA Porton down UK 1 6 75003694 8x50 sealed Thermo Scientific 1 Rotor Specifications Thermo Scientific Thermo Scientific Table 1 11 Thermo Scientific 230V 50Hz 60Hz ventilated Centrifuge Thermo Scientific SL 16 Catalog 75004000 Weight empty kg of rotor 3 3 max cycle number 50 000 Maximum permissible load g 8x189 maximum speed Nmax rpm 6700 maximum RCF value at nmax 7177 max min radius cm 14 3 6 9 Pitch angle 45 Accel braking time s 30 35 sample heating at C referred to 12 ambient temperature of 23 C running time 60 minutes Aerosol tight permissible temperature range 121 autoclavable ested HPA Porton down Table 1 12 Thermo Scientific 230V 50Hz 60Hz refrigerated Centrifuge Thermo Scientific SL 16R Catalog 75004030 Weight
6. SUUUSSUUSSSSUU Metrizamide MS S 555 55 5 S S 5 SS S S 5 5 5 55 Lactic Acid 100 5 MS U 555 MS 5 MS MS 5 5 Lactic Acid 20 5 5 MS M 59555555 MSMSS 5 N Butyl Alcohol 5 S U 8 5 M US MS S S S MMS M 5 5 N Butyl Phthalate SSU SSS 51010750 UUUM 00505 MMS US N N Dimethylformamide SS88USMS S8USUSUU SSUUSMSSSU Sodium Borate MS 88 SSS S S 8 88S US 5S8 8 858 85358 SMS SS Sodium Bromide USS SSS SSS SSES 885 8 S 58 858 SMS SS Sodium Carbonate 2 MUS SSS S SS SS S SS SSS SUS S85 8 85 S 85 SSS Sodium Dodecyl Sulfate SSS SSS SSS SS SSS 5555 SSS 85 85 5 Sodium Hypochlorite 5 UUMS MUS SMS 55 555 MS SS MUS MS Sodium lodide MS S SSS SS 5 SSS 5 SS S 5 5 5 5 55 Sodium Nitrate SSS SSSS 555 S 565 5 S 556555 USS 5 5 Sodium Sulfate USS 858 SY S S 835858 88535 88358 S S S SMS SS Sodium Sulfide 5 5 5 5 555 UU 5 55 M Sodium Sulfite SSS SSS S MS 8558 8 SM SS S 5 85 SS 5 5 5 Nickel Salts US 8 5 5 8 S 5 5 55 5 S SS S 5 5 S MS 85 8 Oils Petroleum S S 8 SUSSSSUUMSMUUSSSUSSSS Oils Other S S SMS 58555105 S SUS 65 5 5 55 MS Oleic Acid S 0558 UUS US SMS S85 8 S S S S S MUS MM Oxalic Acid UUMS S SUS S85 8S US US SSS 85 S UMSS Perchloric Acid 10 U U SUU SMM MUMS MM MS U S S Perchloric Acid 70 U U U UU SUMUUMUUUMMUMSUUSUS Phenol 5 USU SMM SUMUUSUMSMSUUSUMMMS Phenol 50 USU SUM SUMUUUUUSUMUUSUUUMS Phosphoric Acid 10 UUMS SSUSSSSU SSSSSSSSSUMUSS Phosphoric Acid conc U UMM U 5 MSUUMMSSSMSMSUMU S Physi
7. bleaching solutions or scrubbing powder Rinse the cavities out thoroughly Use a soft brush without metal bristles to remove stubborn residue Afterwards rinse with distilled water Place the rotors on a plastic grate with their cavities pointing down If drying boxes are used the temperature must never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with the cavities pointing down CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment 6 2 75003694 8x50 sealed Thermo Scientific 6 Maintenance and Care Disinfection Clean centrifuge and accessories as follows 1 Open the centrifuge Turn off the centrifuge Pull out the power supply plug Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Remove the centrifuge tubes and adaptors Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning CO d UU N Dry all of the rotors and accessorie after cleaning with a cloth or in a
8. radius 14 3 6 9 14 3 6 9 Pitch angle 45 45 Accel braking time s 30 35 30 35 sample heating at C referred to 12 12 ambient temperature of 23 C running time 60 minutes Aerosol tight yes yes permissible temperature range 121 121 autoclavable C ested HPA Porton down UK Table 1 6 Sorvall 230V 50Hz 60Hz refrigerated Centrifuge Sorvall Legend X1R Sorvall ST 16R Catalog 75004260 75004380 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8x189 8x189 maximum speed nmax rpm 6700 6700 maximum value at nmax 7177 7177 max min radius cm 143 6 9 143 6 9 Pitch angle 45 45 Accel braking time s 30 35 30 35 Aerosol tight yes yes permissible temperature range 121 121 autoclavable C Tested by HPA Porton down UK 1 4 75003694 8x50 sealed Thermo Scientific Table 1 7 Sorvall 120V 60Hz ventilated 1 Rotor Specifications Sorvall Centrifuge Sorvall Legend X1 Sorvall ST 16 Catalog 75004271 75004241 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8 189 8 189 maximum speed nmax rpm 6500 6500 maximum RCF value at nya 6755 6755 max min radius cm 143 6 9 14 3 6 9 Pitch angle 45 45 Accel braking time s 40 40 40 40 sample heating at nmax C referred to 11 11 ambient tempe
9. rotor and accessories thoroughly with water Dispose of the decontamination solution according to the applicable guidelines 10 Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the boldof the swing out rotor with bold grease 75003786 Before autoclaving clean rotor and accessories and described above Place the rotor on a flat surface Rotors and adapter can beautoclaved at 121 C The maximum permissible autoclave cycle is 20 minutes at 121 C Clean the rotor before autoclaving and rinse it with distilled water Remove all accessories tubes adapters from the rotor Place the rotor on a flat surface Note No chemical additives are permitted in the steam CAUTION Never exceed the permitted temperature and duration when autoclaving If the rotor shows signs of corrosion or wear it must be replaced Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers i
10. warm air cabinet at a maximum temperature of 50 C 8 Clean the housing of the centrifuge as needed After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the boldof the swing out rotor with bold grease 75003786 CAUTION When cleaning do not allow liquids especially organic solvents to get on the drive shaft the bearings the AutoLock or the locks Organic solvents break down the grease in the motor bearing The drive shaft could freeze up After some applications their might be ice in the rotor chamber Let the ice melt and drain it off Clean the rotor chamber as described above Disinfection Thermo Scientific Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts immediately Take other precautions if need be Use a sprayer whenever possible so that all surfaces are covered evenly The rotor chamber and the rotor should be treated preferably with a neutral disinfectant A disinfectant spray would be most suitable for this purpose so that the rotor and accessory surfaces are covered
11. 00 6 9 14 3 1852 3839 5000 6 9 14 3 1929 3997 5100 6 9 14 3 2006 4158 5200 6 9 14 3 2086 4323 5300 6 9 14 3 2167 4491 5400 6 9 14 3 2249 4662 5500 6 9 14 3 2334 4836 5600 6 9 14 3 2419 5014 5650 6 9 14 3 2463 5104 5800 6 9 14 3 2595 5378 5900 6 9 14 3 2685 5565 6000 6 9 14 3 2117 5755 6100 6 9 14 3 2870 5949 6200 6 9 14 3 2965 6146 6300 6 9 14 3 3062 6345 6400 6 9 14 3 3160 6548 6500 6 9 14 3 3259 6755 6600 6 9 14 3 3360 6964 6700 6 9 14 3 3463 7177 75003694 8x50 sealed Thermo Scientific Chemical Compatibility Chart 2 g 5 E E z z 5 t 2 TT E iib 111032 2 S02 298 222 2 mercaptoethanol 5 5 U SMS SUSSUSS 5555 US 8 8 5 5 5 Acetaldehyde U U MU UU MS U U Acetone MS U 5 MS 5 UUS US UUUS S US S U U Acetonitrile SSU SMS S 5 US UMU U SMUU S SS 5 UU Alconox UUS SSS 555555 MSSSS S 358 SS S SS U Alcohol s lt S SSMSSS MS T Aluminum Chloride UUSSSSUSSSSMSSSS SSSSSMUUSS Formic Acid 100 5 MU U U SMUUS 5 U 5 U S U Ammonium Acetate 5 S U 555 SSS S 5 5 S U 5555558555535 Ammonium Carbonate MS 556565 565656556565 SSS SE 85 S MSSS Ammonium Hydroxide 10 UUS US SMS S85 8 SUMS SS S S S S S SMS Ammonium Hydroxide 28 UUS US UMS 885 S USUMS SS 85 S S S SMS Ammonium Hydroxide UU UU S UMS S SUS UU SSS MS SSS U Ammonium Phosphate U 5 SS S 5 85 5585 5 SSM 858 835 SMS 58558 Ammonium Sulfate
12. 1 4 Sorvall 120V 60Hz ventilated 1 5 Sorvall 120V 60Hz reftrigerated ua suaiaqanahu 1 5 Sorvall 100V 50 60Hz ventilated 1 6 Sorvall 100V 50 60Hz refrigerated 1 6 Thermo Sot EO u s a aaa Abas 1 7 Thermo Scientific 230V 50Hz 60Hz ventilated 1 7 Thermo Scientific 230V 50Hz 60Hz refrigerated 1 7 Thermo Scientific 120V 60Hz ventilated a 1 8 Thermo Scientific 120V 60Hz refrigerated 1 8 Chapter2 Accessories u u earner 2 1 Chapter3 lll ttt 3 1 TP 3 2 Removing th rOt r 3 3 berti dece 4 1 Betorea RUD MNT 4 2 Proper Loading at sanan diac 4 2 proper Loading aeu aaa q qua Gu enoi Ce STC aaa eiia are 4 2 Maximum Loading hanya antennae 4 3 Cycle teats 4 3 Chapter5 Aerosol tight Applications I U uu u 5 1 Basic Wet Hs 5 2 lu AST D m TT X 5 2 BE M 5 2 Checking the Aeros
13. 400 Belgium 32 2 482 30 30 Germany 08001 536 376 49 6184 90 6940 France 33 2 2803 2180 33 2 2803 2000 Italy 39 02 02 95059 341 Netherlands 31 76 571 4440 Nordic Baltic Countries 35 89 329 100 Russia 7 812 703 42 15 Thermo Scientific 75003694 8x50 sealed C 1 C Contact Information Spain Portugal Switzerland UK Ireland China 34 932 23 09 18 34 932 23 09 18 41 44 454 12 12 44 870 609 9203 86 21 6865 4588 India Japan Other Asian Countries Latin America Other Countries 86 10 8419 3588 91 22 6716 2200 81 45 453 9220 852 2885 4613 1 866 984 3766 49 6184 90 6940 0 2 75003694 8x50 sealed 433 2 2803 2180 Thermo Scientific Index A AJACCESSOEIGS 2 1 Aerosol tight Applications 5 1 Aerosol tight rotors 3 3 POLL e P 6 5 B Basic 5 2 Before a 4 2 C 6 1 Checking the Aerosol Tightness 5 2 Chemical Compatibility Chart B 1 paa Mo adi 6 2 Cleaning agents 6 3 Contact Information C 1 0001 025 ansa resents 4 3 D Decontamination 6 4 Disinfection 2 6 3 F Fill Level 5 2 H Heraeus 120V 60Hz refrigerated 1 3 Heraeus 120V
14. 50 000 Maximum permissible load g 8 189 8 189 maximum speed Nmax rpm 6500 6500 maximum RCF value at nmax 6755 6755 max min radius cm 14 3 6 9 14 3 6 9 Pitch angle 45 45 Accel braking time s 40 40 40 40 sample heating at nmax C referred to 11 11 ambient temperature of 23 running time 60 minutes Aerosol tight yes yes permissible temperature range 121 121 autoclavable C Table 1 4 Heraeus 120V 60Hz refrigerated ested Porton down Centrifuge Heraeus Multifuge X1 R Heraeus Megafuge 16R Catalog 75004251 75004271 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8 189 8 189 maximum speed Nmax rpm 6700 6700 maximum RCF value at nmax 7177 7177 max min radius cm 14 3 6 8 14 3 6 8 Pitch angle 45 45 Accel braking time s 40 40 40 40 Aerosol tight yes yes permissible temperature range 121 121 autoclavable C ested by HPA Porton down Thermo Scientific 75003694 8x50 sealed 1 3 1 Rotor Specifications Sorvall Sorvall Table 1 5 Sorvall 230V 50Hz 60Hz ventilated Centrifuge Sorvall Legend X1 Sorvall ST 16 Catalog 75004220 75004240 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8x189 8x189 maximum speed Nmax rpm 6700 6700 maximum RCF value at nra 7177 7177 max min
15. 694 8x50 sealed 3 1 3 AutoLock Rotor installation Rotor installation CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge This rotor is equipped with an AutoLock system This system is used to automatically lock the rotor to the centrifuge spindle The rotor does not have to be bolted onto the centrifuge spindle Proceed as follows 1 Open the door of the centrifuge and if necessary remove any dust foreign objects or residue from the chamber AutoLock and o ring must be clean and undamaged AutoLock O ring Figure 3 1 AutoLock 2 Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle The rotor clicks automatically into place CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then it may be necessary to press it onto the centrifuge spindle with a small amount of pressure 3 Check if the rotor is properly installed by lifting it slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle WARNING If the rotor cannot be properly locked in place after several attempts then the AutoLoc is defective and you are not permitted to operate the rotor Check for any damage to the rotor Damaged rotors must not be used Keep the hub area clear of objects 3 2 75003694 8 50 sealed Thermo Scientific 3 AutoLock Remo
16. R GLASS THRMOSET c POLYTHERMIDE co POLYVINYL CHLORIDE a a a RULONA TEFLON Glutaraldehyde N co lt lt CELLULOSE ACETATE BUTYRATE Glycerol Guanidine Hydrochloride Haemo Sol Hexane vc c c c AUINUM a c aj co S c ANODICCOATING for ALUMINUM MAI Mm nD nD co clol m a a co CO co co an Mm a Z lt C STAINLESS STEEL Isobutyl Alcohol Isopropyl Alcohol lodoacetic Acid Potassium Bromide Potassium Carbonate Potassium Chloride Potassium Hydroxide 5 MAI nm nn nm co MI DA nn co WD MW WM WM NM c c NYLON co co m co a a a aj c C SIICONERUBBER Potassium Hydroxide conc C aj CO nM lt Potassium Permanganate Calcium Chloride Calcium Hypochlorite c co C C C Z MN aj lt CO CO CO MAI MD MD MD DA DDN lt co Kerosene lt lt CO CO CO YM FIC C A WM YM YM co C NEOPRENE MA ML MA WM TI ND DANA A NA A NDA NA NA co Sodium Chloride 10 S Sodium Chloride sat d Carbon Tetrachloride Aqua Regia C co gt Mm Mm WMD WD nD co 0 lt Mm mA Wm C
17. RULONA TEFLON A 9 NM Al co co c cc nm C a co C TITANIUM lt m Z al ajal m m wm n n lt co aj ajaj c aj ajaj ajaja co co co aj ajaj co co aj 0 ajaj o nm o c coco ajl ajaj lt vaj co 0 m vaj co co co oco c Z vaj aj oaj c aj Citric Acid 10 Thermo Scientific 75003694 8x50 sealed B 5 B chemical Compatibility Chart Polyethyleneterephthalate Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material Chemical resistance data is included only as a guide to product use Because no organized chemical resistance data exists for materials under the stress of centrifugation when in doubt we recommend pretesting sample lots B 6 75003694 8x50 sealed Thermo Scientific Contact Information United States 866 9 1 866 984 3766 Canada 1 866 984 3766 Austria 43 1 801
18. Replace the rotor when the specified number of cycles is reached Due to the mechanical load a rotor can break and thus damage the centrifuge Service life examples Usage profile Maximum service life at 50 000 cycles frequent use 7 years 30 runs day 220 days year 4 4 75003694 8x50 sealed Thermo Scientific Aerosol tight Applications Thermo Scientific 75003694 8x50 sealed 5 1 5 Aerosol tight Applications Basic Principles Basic Principles CAUTION When centrifuging hazardous samples do not open aerosol tight rotors or buckets unless placed in a safety cabinet Always bear in mind the maximum permitted fill levels Be sure to check all seals before starting any aeorsol tight applications Check that the sample containers are well suited for the desired centrifugation process Placing O ring The o ring fullfils its purpose best when it is neither stressed nor bulked Meaning the o ring should be equally placed in the groove of the lid Place the o ring as follows 1 Place the o ring above the groove 2 Push the o ring on two opposite places into the groove Make sure the rest of the o ring is equally distributed 3 Push the centers of the loose parts into the groove 4 Push the remaining o ring into place Note If the o ring seems to be too long or too short take it off the lid and repeat the process Fill Level The tubes are only to be filled to a level which ensures that the sa
19. UMS SS US S SS S S S SE S S 55 55 5 S US 501 Amyl Alcohol 5 MU 5 5 M S MS 555 U S M Aniline SS UUS US MS UUUUUUU 5 MUUS 555 US Sodium Hydroxide 196 U MS 55 5 MS 5 SMMS S SSS SMS 5 U Sodium Hydroxide 1096 U MU U MMS S US UUS 8 8 S S SMS 5 0 Barium Salts MUS SSS 5 5 5585 SS S SM S358 835 SMS SSS Benzene 550105 05 005 UUUMUMUUUS U US US Benzyl Alcohol S UU M M M S U UU U U U U MS M 5 5 Boric Acid US SMS SUS S85 85 8 85 85 SUS S SS 8585 5 SSS Thermo Scientific 75003694 8x50 sealed B 1 B chemical Compatibility Chart 5 2 5 5 8 ae m E E og 2115 CHEMICAL z EE Cesium Acetate M 5 5 5 5 5 5 5 9 55 8 5 5 5 5 5 MS S5 Cesium Bromide MS S 5 5 5 8 5 5 5 85 355 8 5 5 5 5 5 MS 8 Cesium Chloride 55105855 5 5 655 65 5 5 S 8 5 5 5 5 5 MS 8 8 Cesium Formate MSS SSS 855535355 5 S o o o o 5 MS 8 8 Cesium lodide MSS SSS 8555555 5S S 5 S SMS 8 S Cesium Sulfate MS S SS S 85555535 5 5 o 5 5 5 5 MS 8 5 Chloroform UUUUS SMUSUUMUMUUUMMUUSUUUMS Chromic Acid 1096 U U US UU 5565 US SMUMS SUMSMUSSS Chromic Acid 50 U U U U U SUUSMUMSSUMS U M S Cresol Mixture S 5 U S SUUUUUU U U US 5 5 SUS Cyclohexane 8 5 5 555 US US S UUUMS MUMMS UMMU 5 Deoxycholate 5 5 5 S O 5 5 85 85 85 5 5 9 S 5 5 5 85 O 85 5 5 9 Distilled Wat
20. empty kg of rotor 3 3 max cycle number 50 000 Maximum permissible load g 8x189 maximum speed Nmax rpm 6700 maximum RCF value at nmax 7177 min radius cm 14 3 6 9 Pitch angle 9 45 Accel braking time s 30 35 Aerosol tight yes permissible temperature range 121 autoclavable C ested APA Porton down Thermo Scientific 75003694 8x50 sealed 1 7 1 Rotor Specifications Thermo Scientific Table 1 13 Thermo Scientific 120V 60Hz ventilated Centrifuge Thermo Scientific SL 16 Catalog 75004001 Weight empty kg of rotor 3 3 max cycle number 50 000 Maximum permissible load g 8 189 maximum speed nmax rpm 6500 maximum value at nmax 6755 max min radius cm 14 3 6 9 Pitch angle 45 Accel braking time s 40 40 sample heating at nmax C referred to 11 ambient temperature of 23 C running time 60 minutes Aerosol tight yes permissible temperature range 121 autoclavable C Tested by HPA Porton down UK C Table 1 14 Thermo Scientific 120V 60Hz refrigerated Centrifuge Thermo Scientific SL 16R Catalog 75004031 Weight empty kg of rotor 3 3 max cycle number 50 000 Maximum permissible load g 8x189 maximum speed nmax rpm 6700 maximum RCF value at Nmax 7177 min radius 14 3 6 9 Pitch angle 45 Accel braking time s 40 40 Aerosol tight yes
21. er S 5 5 5 85 S 85 85 8 8 8 85 88 88 8 8 8 8 8 8 8 8 8 8 5 Dextran 5 5 5 9 535 8 5 59 8858585 5 5 55 5 5 55 MS S S Diethyl Ether S88uUuUuusssusuusuuuuuuuuussssMUu Diethyl Ketone S U U M S U S MU UU MM U S S UU Diethylpyrocarbonate 5 5 U 5 5 5 o U S U S U 555 MS 8 8 8 5 5 Dimethylsulfoxide 55 U US 8 8 S US S US D U 55 UU S 85585501 Dioxane MS UU S S MMS U US U MU U MMMU 5 555001 Ferric Chloride UU S MS M S S 5 5 MU S 5 Acetic Acid Glacial S88UuUuussuMsSusuuuuuMSsSuMusSsuus U Acetic Acid 5 SSMS 88 MS 88 55 MS 88 88 8S8 MS SMS SM Acetic Acid 60 55 UU S SU SMSUUMUSMSMSMSMUSMU Ethyl Acetate MMUUSSMMSSUSUMUWU 85105 MMS U U Ethyl Alcohol 50 959585 5 5 MS S 8 8 S US US S88 8 8 SSS MS MU Ethyl Alcohol 95 S S US SMS S S 5 S US U 555 MS 5505 MU Ethylene Dichloride S U U S M U U 5 U UJ U U lU U US 0 5 5 Ethylene Glycol 8 5 5 5 85 8 85 8 8 8 8 8 s U SS S S 58558555 MS MS Ethylene Oxide Vapor S U U S U 5 5 M 59595 US US 85501 Ficoll Hypaque MS S 8 5 5 85 5 5 85 88 85 8 88 88 585 MS 8 8 B 2 75003694 8x50 sealed Thermo Scientific CHEMICAL Hydrofluoric Acid 10 MATERIAL POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy ETHYLENE PROPYLENE GLASS B Chemical Compatibility Chart POLYCLEAR CLEARCRIMP CCCLEARCRIMP TYGON VITON Hydrofluoric Acid 50 Co POLYRTHYLENE POLYSULFONE lt Hydrochloric Acid conc Formaldehyde 4096 CC C POLYESTE
22. evenly CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Observe the safety precautions and handling instructions for the cleaning agents used 75003694 8x50 sealed 6 3 6 Maintenance and Care Decontamination Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants Disinfect the rotor and accessories as follows 1 Open the centrifuge Turn off the centrifuge Pull out the power supply plug 2 3 4 Grasp the rotor with both hands and lift it vertically off the centrifuge spindle 5 Remove the centrifuge tubes and adaptors and dispose of them or disinfect them 6 Treat the rotor and accessories according to the instructions for the disinfectant spray or soak in solution Adhere strictly to the given application times sure the disinfectant can drain off the rotor 8 Rinse the rotor and rotor lid thoroughly with water and then rub down 9 Dispose of the disinfectant according to the applicable guidelines 10 Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the boldof the
23. ilated Centrifuge Heraeus Multifuge X1 Heraeus Megafuge 16 Catalog 75004210 75004230 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8x189 8 189 maximum speed rpm 6700 6700 maximum RCF value at nmax 7177 7177 min radius cm 14 3 6 9 14 3 6 9 Pitch angle 45 45 Accel braking time s 30 35 30 35 sample heating at C referredto 12 12 ambient temperature of 23 running time 6 minutes Aerosol tight yes yes permissible temperature range 121 121 autoclavable C ested Dy HPA Porton down UK Table 1 2 Heraeus 230V 50Hz 60Hz refrigerated Centrifuge Heraeus Multifuge X1 R Heraeus Megafuge 16R Catalog 75004250 75004270 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8x189 8x189 maximum speed Nmax rpm 6700 6700 maximum RCF value 7177 7177 min radius 143 6 9 143 6 9 Pitch angle 45 45 Accel braking time s 30 35 30 35 Aerosol tight yes yes permissible temperature range 121 121 autoclavable C ested by Porton down UK 1 2 75003694 8x50 sealed Thermo Scientific Table 1 3 Heraeus 120V 60Hz ventilated 1 Rotor Specifications Heraeus Centrifuge Heraeus Multifuge X1 Heraeus Megafuge 16 Catalog 75004211 75004231 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000
24. lowing general safety regulations must be followed Do not remove the magnet at the rotor bottom Do not use rotors which show any signs of corrosion and or cracks Useonly with rotors that have been loaded properly Never run the rotor with missing stainless steel tube holders Never overload the rotor Use only accessories which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they have been approved for the speed or the RCF value of the rotor Thermo Scientific 75003694 8x50 sealed iii Preface Please observe the safety instructions Please pay particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 125 m This symbol refers to general hazards ATTENTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to biological hazards Observe the information contained in the instruction manual to keep yourself and your environment safe iv 75003694 8x50 sealed Thermo Scientific Rotor Specifications Thermo Scientific 75003694 8x50 sealed 1 1 1 Rotor Specifications Heraeus Heraeus Table 1 1 Heraeus 230V 50Hz 60Hz vent
25. mple is unable to reach the top of the tube during centrifugation Therefore fill the tube only 2 3 of the rated level Checking the Aerosol Tightness The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in accordance with the EN 61010 2 020 Appendix AA Whether or not a rotor is aerosol tight depends primarily on proper handling Check as needed to make sure your rotor is aerosol tight The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks scratches and embrittlement is extremely important Aerosol tight applications are not possible if the lids are open 5 2 75003694 8x50 sealed Thermo Scientific Ouick Test Thermo Scientific 5 Aerosol tight Applications Checking the Aerosol Tightness Aerosol tightness requires the correct operation when filling the sample vessels and closing the rotor lid As a quick test it is possible to test the aerosol tight buckets using the following process Lubricate all seals lightly Always use the special grease 7600 3500 when lubricating the seals Fill the bucket with approx 10 ml of carbonated mineral water Close the bucket as explained in the handling instructions Shake the bucket vigorously using your hands This releases the carbonic acid gas which is bound in the water resulting in excess pressure Do not apply pressure to the lid when doing so Leaks can be detected by esca
26. nspection and service contracts for this work Thermo Scientific 75003694 8x50 sealed 6 5 RCF Values Speed rpm Rinax RCF RCF 300 6 9 14 3 7 14 400 6 9 143 12 26 500 6 9 14 3 19 40 600 6 9 14 3 28 58 700 6 9 14 3 38 78 800 6 9 14 3 49 102 900 6 14 3 62 129 1000 6 9 14 3 77 160 1100 6 9 14 3 93 193 1200 6 9 14 3 111 230 1300 6 9 14 3 130 270 1400 6 9 14 3 151 313 1500 6 9 14 3 174 360 1600 6 9 14 3 197 409 1700 6 9 143 223 462 1800 6 9 14 3 250 518 1900 6 9 14 3 278 577 2000 6 14 3 309 639 2100 6 9 143 340 705 2200 6 9 14 3 373 774 2300 6 9 14 3 408 846 2400 6 9 143 444 921 2500 6 9 14 3 482 999 2600 6 9 14 3 521 1081 2700 6 9 143 562 1165 2800 6 9 14 3 605 1253 2900 6 9 14 3 649 1345 3000 6 9 14 3 694 1439 Thermo Scientific 75003694 8 50 sealed A 1 A RCF Values A 2 Speed rpm ir ET RCF Rmin RCF 3100 6 9 14 3 741 1536 3200 6 9 14 3 790 1637 3300 6 9 14 3 840 1741 3400 6 9 14 3 892 1848 3500 6 9 14 3 945 1958 3600 6 9 14 3 1000 2072 3700 6 9 14 3 1056 2189 3800 6 9 14 3 1114 2309 3900 6 9 14 3 1173 2432 4000 6 9 14 3 1234 2558 4100 6 9 14 3 1297 2687 4200 6 9 14 3 1361 2820 4300 6 9 14 3 1426 2956 4400 6 9 14 3 1493 3095 4500 6 9 14 3 1562 3237 4600 6 9 14 3 1632 3383 4700 6 9 14 3 1704 3532 4800 6 9 14 3 1777 3683 49
27. oacetic Acid aj o z c c c C C aj aj C C STAINLESS STEEL c o Zz wm a a co co Mi na co lt lt lt lt lt lt o o o c DERI c c z lt Trichloroethane c c C C C a c C c mM WM co cc co c POLYVINYL CHLORIDE lt c c c c c z c c aj aj a BUNAN co a aj c c c c c c c c c aj aj a NYLON c c c a c c a C c c POLYCARBONATE c c c c c C c c a c c a a c SILICONE RUBBER GIG GC C V cn 2 Wn 2 gt CI C gt l lt Trichloroethylene Trisodium Phosphate 26019 Tris Buffer neutral pH Triton X 1009 C anl co Hydrogen Peroxide 10 Hydrogen Peroxide 3 Xylene CO Zinc Chloride Zinc Sulfate MN cO mM C cO co c c lt cC C wm a POLYALOMER ecc c co cC wm C a C cO C POLYRTHYLENE A c C c lt lt Z c cO A CO PODYPROPYLENE co co nl al nl al nl al nl al nl aml nl aml wm wm co co aj wm a a a Co
28. ol Tightness ananassa 5 2 Thermo Scientific 75003694 8 50 sealed i Contents Chapter6 Maintenance and Care l uu tente tnnt 6 1 leaning 6 2 Cleaning z nn ente We cet e ass 6 2 u aa G cls 6 3 Decontamination aun eie pei te ueteres 6 4 AG0tOc ayt6 tod Aetio au beato 6 5 Service of Thermo Fisher Scientific iu soe Rost nana 6 5 EID mI A 1 Chemical Compatibility Chart senes B 1 Contact Informatii ee C 1 ii 75003694 8x50 sealed Thermo Scientific Preface Before starting to use the rotor read throughthis instruction manual carefully and follow the instructions The information contained in this instruction manual is the property of Thermo Fisher Scientific it is forbidden to copy or pass on this information without explicit approval Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the seller s warranty Scope of Supply Article Number Quantity Check 75003694 8x50 sealed 1 L 50121058 Instruction Manual 1 0 If any parts missing please contact the nearest Thermo Fisher Scientific representative Precautions In order to ensure safe operation of the 8x50 sealed the fol
29. ologic Media Serum Urine MS SS S SSS SS 585558 5 5 5 5 555 5 5 5 5 Picric Acid SSU SMS SS MS 0555 05555 US UMS MS B 4 75003694 8x50 sealed Thermo Scientific B Chemical Compatibility Chart CHEMICAL MATERIAL ETHYLENE PROPYLENE O coco Z C C C C lt ALUMINUM CELLULOSE ACETATE BUTYRATE POLYTHERMIDE POLYESTER GLASS THERMOSET Pyridine 5096 Rubidium Bromide Rubidium Chloride Sucrose co co POLYSULFONE Sucrose Alkaline a a a C PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP Sulfosalicylic Acid Nitric Acid 10 mM S WM WM WM Co ANODIC COATING for ALUMINUM Nj NM WM CO CO WM Co POLYURETHANE ROTOR PAINT Mm mM aj aj CO CO CO GLASS z co TYGON Nitric Acid 50 Nitric Acid 95 Hydrochloric Acid 10 Hydrochloric Acid 50 Sulfuric Acid 1096 c C a co a co NEOPRENE nin rn nln CO CO DM CO CO CO YM CO C NM YM YD lt coco co VITON Sulfuric Acid 50 c c c c a c C C a lt WM a a c COMPOSITE Carbon Fiber Epoxy gt cc c C lt Sulfuric Acid gt Stearic Acid Tetrahydrofuran Toluene lt lt Trichlor
30. permissible temperature range 121 autoclavable C ested HPA Porton down UK 1 8 75003694 8x50 sealed Thermo Scientific jJ_ Accessories Contents Rotor data on page 2 2 e Accessories on page 2 2 Thermo Scientific 75003694 8x50 sealed 2 1 2 Accessories Thermo Scientific General Purpose Rotors 8 50ml Individually Sealed Fixed Angle Rotor 8 x 50 Individually Sealed Rotor Rotor Specifications Capacity ml 8x50 Tube Dimensions mm 17 x 121 Angle 45 Maximum Speed rpm 6700 K Factor 4107 Net Weight kg 3 3 Max Speed may vary depending on centrifuge unit RCF Radius RCF x g Radius cm Maximum 7 177 14 3 3 459 6 9 Maximum Speed vary by centrifuge model 8 x 50ml Individually Sealed Fixed Angle Rotor Cat No Description 75003694 Accessories 8 x 50ml Individually Sealed Fixed Angle Rotor Cat No Description 75003789 O ring Refresher Kit Tube Ordering Information Sealing Assembly Required Adapters Required Accessories Required Tube Vol Fill Max Cat No Description Qty Speed Qty Qty No of Vol ml ml mm ipti ml ml rpm mm Cat No IPkg Description IPkg Places 50 50 Disposable Conical 17 x 121 2 2 75003694 8x50 sealed Thermo Scientific AutoLock M Contents Rotor installation on page 3 2 Removing the rotor on page 3 3 Thermo Scientific 75003
31. ping water or the sound of escaping gas Replace the seals if you detect any leaks Then repeat the test Dry the rotor rotor cover and the cover seal CAUTION Prior to each use the seals in the bucket are to be inspected in order to assure that they are correctly seated and are not worn or damaged Damaged seals are to be replaced immediately When loading the rotor ensure that the rotor lid closes securely Damaged or clouded rotor covers are to be replaced immediately 75003694 8x50 sealed 5 3 Maintenance and Care Thermo Scientific 75003694 8x50 sealed 6 1 6 Maintenance and Care Cleaning intervalls Cleaning intervalls For the sake of personal environmental and material protection it is your duty to clean and if neces sary disinfect the centrifuge on a regular basis Maintenance Recommended interval Clean rotor chamber daily or when polluted Clean rotor daily or when polluted Accessories daily or when polluted Cabinet month Ventilation holes Every six months Cleaning CAUTION Refrain from using any other cleaning or decontamination procedure than those recommended here if you are not entirely sure that the intended procedure is safe for the equipment Use only approved cleansers If in doubt contact Thermo Fisher Scientific When cleaning centrifug Use warm water with a neutral solvent Never use caustic cleaning agents such as soap suds phosphoric acid
32. rature of 23 C running time 60 minutes Aerosol tight yes yes permissible temperature range 121 121 autoclavable C Table 1 8 Sorvall 120V 60Hz refrigerated Tested by Porton down Centrifuge Sorvall Legend X1R Sorvall ST 168 Catalog 75004261 75004381 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8x189 8x189 maximum speed Nmax rpm 6700 6700 maximum RCF value at nmax 7177 7177 max min radius cm 143 6 9 143 6 9 Pitch angle 45 45 Accel braking time s 40 40 40 40 Aerosol tight yes yes permissible temperature range 121 121 autoclavable C ested by Porton down UK Thermo Scientific 75003694 8x50 sealed 1 5 1 Rotor Specifications Sorvall Table 1 9 Sorvall 100V 50 60Hz ventilated Centrifuge Sorvall Legend 1X Sorvall ST 16 Catalog 75004223 75004243 Weight empty kg of rotor 3 3 3 3 max cycle number 50 000 50 000 Maximum permissible load g 8x189 8x189 maximum speed Nmax rpm 6 000 6 000 maximum value at nmax 5 755 5 755 min radius cm 14 3 6 9 14 3 6 9 Pitch angle 45 45 Accel braking time s 35 35 35 35 sample heating at Nmax C referredto 10 10 ambient temperature of 23 C running time 60 minutes Aerosol tight yes yes permissible temperature range 121 121 autoclavable C T sted Dy
33. rinted in October 2009 For Research Use Only Not for use in diagnostic procedures Centre of Emergency Preparedness and Response Health Protection Agency Porton Down Salisbury Wiltshire SP4 0JG United Kingdom Certificate of Containment Testing Containment testing of Thermo Scientific Vessel 75003787 Report No 77 08 B Report prepared for Thermo Fisher Issue Date 1 June 2009 Test Summary A Thermo Scientific vessel 75003787 with aerosol tight lid Max rcf 7177 x g was supplied by Thermo Fisher and containment tested at max rcf 7177 x g using the method described in Annex AA of EN 61010 2 020 The vessel was shown to contain a spill when tested in triplicate Report Written By Report Authorised By 410 MEN uU Table of Contents Prelat mmt RENE iii ili ili Chapter 1 Rotor Specifications I U U 1 1 lec AE 1 2 Heraeus 230V 50Hz 60Hz ventilated 1 2 Heraeus 230V 50Hz 60Hz refrigerated 1 2 Heraeus 120V ventilated aues nico 1 3 Heraeus 120V 60Hzrefrigerated 1 3 Sorvall 1 4 Sorvall 230V 50Hz 60Hz ventilated 1 4 Sorvall 230V 50Hz 60Hz refrigerated
34. swing out rotor with bold grease 75003786 Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation WARNING Radioactive material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts immediately Take other precautions if need be CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment For general radioactive decontamination use a solution of equal parts of 7096 ethanol 1096 SDS and water Disinfect the rotor and accessories as follows 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug 6 4 75003694 8x50 sealed Thermo Scientific QN Autoclaving 1 2 6 Maintenance and Care Autoclaving Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Remove the centrifuge tubes and adaptors and dispose of them or disinfect them Rinse the rotor first with ethanol and then with de ionized water Adhere strictly to the given application times Be sure the decontamination solution can drain off the rotor Rinse the
35. ving the rotor CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it a its handle Be sure to check all seals before starting any aeorsol tight applications 4 Close the centrifuge door Removing the rotor remove the rotor proceed as follows 1 Open the centrifuge door 2 Grab the rotor handle with both hands and press against the green AutoLock key At the same time pull the rotor directly upwards with both hands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this Thermo Scientific 75003694 8x50 sealed 3 3 Rotor loading Thermo Scientific 75003694 8x50 sealed 4 1 4 Rotor loading Before a Run Before a Run 1 Please read and observe the safety instructions contained in these operating instructions and in the instructions for use 2 Check the rotor and all accessory parts for damages such as cracks scratches or traces of corrosion 3 Check the rotor chamber the motor shaft and the 4 Check the rotor s suitability using the Chemical Compatibility Chart on page B 1 5 Make sure the tubes or bottles do not touch the lid Proper Loading To ensure safe operation of the centrifuge the rotor must be evenly loaded at all times All places must contain stainless steel tube holders Running the rotor with missing stainless steel tube holders is forbidden

8 x 50mL Individually Sealed Rotor for General

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