HistoPro 3030 H & E Stainer - Triangle Biomedical Sciences
Contents
1. 00 01 Low High Carrier Pickup Speed Ho Normal Fast Previous Step Figure 6 3 Editing Program Steps Screen Show Next Step 6 The station at which the process is performed can be programmed under Station The station number be incremented or decremented by pressing the and buttons until the desired station number is displayed Stations are numbered in the following way Note The following station numbers apply when the operator is facing the front of machine Figure 6 4 Station Numbering Station Features Samples are loaded at Load Station 30 and can be unloaded at Unload Stations 21 or Unload Station 22 The station number time at station agitation criticality of a process can be programmed for all programmable stations Stations 1 20 23 29 The rinse stations are located at Stations 5 9 Station 9 has a separate water feed inlet and Stations 5 8 are fed from one common inlet The www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 25 10 11 12 13 temperature can be controlled for processes that occur at Heat Stations 10 and 20 Carrier pick up speed can be programmed for processes that occur at Stations 1 9 11 19 and 23 30 The duration for which the process is performed can be programmed under Time at Station m s Duration time refers to the time for which the sample is completely submerged in the station vessel The p2. Tes TRIANGLE BIOMEDICAL SCIENCES INC 3014 CROASDAILE DRIVE DURHAM NORTH CAROLINA 27705 2507 USA 919 384 9393 FAX 919 384 9595 E MAIL tbs trianglebiomedical com HistoPro 3030 H amp E Stainer Operator s Manual v1 0 www trianglebiomedical com INNOVATIONS FOR SCIENCE TABLE OF CONTENTS Chapter Introduction 6 eni iere gests De eri eer e o e PR PEUPLE RE 4 Warranty THT ORM AION occ o ed rrr tope tas socie qeu bep koe teo on euentu ctas did ny sed asus 4 Definition of Various Symbols n Leid ei P ei FS Desa nta cR de 5 Product Descriptions n FR 6 Pine tional Description Ol Parts 245 9 oA oos ie hcp aa peo uev Ubi ia adu cese Ba Gau eU meee 7 Chapter2 661110108 11 Electrical Gharaeteristi 0 11 Physical War acteristics nate tt or iade suut e is des a Und dus dtd folds 11 Environmental Conditions ES 12 Iustallabon Cate BOEV ace lis 12 Pe UGTA AM Se Sct ae ce cuc T 12 Chapter 3 Installation INStr cti ns eonna eeraa a o eera E e EEE TEPE RE Ere 13 13 Ins eee sll vas 14 Components Checklist 14 Installation Procedufesuct adocmf occidi Det dolo tube te Reacts aee 14 Chapter 4 Operating Instructions de eee Dette esate d Iva e Pe e a e eee E Eee dee teense 15 Operating the HistoPro 3030 Stainer Le 15 15 Procedure n 15
3. Unload Stations Flow Control Valves Heating Stations Up Down Mechanism In Out Mechanism Charcoal Filter Computer Display Rinse Water Inlets Drain Connection Stations 1 4 11 19 23 29 Stations 5 8 with a common water inlet and station 9 with a separate water inlet Station 30 Stations 21 and 22 Flow Control Valves can control the flow rate of the rinse water Each rinse station has its own dedicated valve The water flow can be completely shut off to any station if you desire to use it as a regular reagent station Stations 10 and 20 are used for heating the slides The heater at the heating station will not turn ON if you select ambient temperature for a given program step in the heating station This will allow you to use the heating station as a regular reagent station The slide rack mounts on the bracket mounted on this mechanism At times this mechanism is referred to as Z axis mechanism also The up down mechanism mounts on this mechanism At times this mechanism is referred to as Y axis mechanism also The charcoal filter is located behind the top left bezel To access the filter pull the bezel straight out in the front The Active Matrix Display provides the information about the status of the system With the touchpad on the display you can program run and maintain the unit with ease There are two water inlets in the rear of the unit Looking from the back of the unit the inlet located to
4. The first step in running a program is to insert a rack of slides to be processed into the Load Station Station 30 as shown in Figure 7 1 Make sure to open the drawer fully properly insert the rack and fully close the drawer The Program Selection Screen appears with the list of the most recently run programs as shown in Figure 7 2 VIEW OTHER l SELECTIONS l If you wish to select a program which is not listed select to view the next group of defined programs You may need to select this button multiple times to cycle through all the program names You can also use the plus and minus buttons to select the first step number to process if you do not want to start at the first step of the program When you have located the program to run and have selected it the program name will appear at the top of the screen to start the processing of the new rack using the selected program definition If you change your mind select the mr button to return to the Main Menu Figure 7 1 Loading a new Rack for Processing in the Load Drawer www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 34 Run this program or select another Start at Step Figure 7 2 Program Selection Screen www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 35 Run Time Status Display While programs are running the screen in Figure 7 3 appears to show the status of each Hi
5. YES NO Time at Station m s Criticality lt 01 00 Carrier Pickup Speed Normal Fast Figure 6 6 Flow Control Station Step Setting 15 To add another step select m A new Editing Program Steps Screen will appear with an incremented Step Number and default settings Follow Steps 8 13 to program newly added steps 16 To browse through and edit each of the defined program steps use the lt and gt arrow buttons to display the previous and next step where applicable 17 Once all desired steps have been programmed select iu A summary screen similar to the screen shown in Figure 6 7 will be displayed www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 28 Program 1 Sample Program Sta Time Temp Agit Crit Flow Spd 1 3 00 No Low Edit Program 2 1 30 Yes Low 10 2 30 55 Low Name 11 2 00 No Low 9 1 00 No Low Edit Step Unload at Station Add New Step Insert New Step Delete Step Unload Station Figure 6 7 Program Steps Summary Screen 18 Racks may be unloaded at Station 21 and 22 To program the station at which racks are Unload unloaded select Station the Program Steps Summary Screen The following screen shown in Figure 6 8 will be displayed NOTE Racks are by default unloaded at Station 21 unless otherwise specified www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 29 Program 6
6. 10 and 20 See Figure 6 5 Temperature refers to the temperature of air located in the station vessel The temperature can be incremented or decremented by pressing the and buttons until the desired temperature is displayed The temperature is programmed as degrees Celsius and ranges from 40 to 70 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 26 Sample Program Step 3 Station Agitation YES NO Time at Station m s Criticality lt Low High gt Figure 6 5 Heating Station Step Setting 14 The flow rate can be programmed under Flow Rate mL min for processes that take place at Rinse Station 9 Flow rate refers to the rate of water flow at the variable flow control station The programmed flow rate can be incremented or decremented by pressing the and buttons until the desired flow rate is displayed The flow rate is programmed as milliliters per minute and can be set from 0 to 2000 in 500 mL min increments It should be noted that the software controlled Flow Rate Adjustment for Station 9 is an optional feature and may not be present in all models Note The flow rate can be manually adjusted for processes that take place at Stations 5 8 with a screwdriver Screwdriver not provided www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 27 Sample Program Step 5 Station Agitation 9
7. New Program Figure 6 8 Unload Station Selection Screen 19 To program Station 21 as the unloading station select To program Station 22 as the unloading station select station The system automatically returns to the previous screen after a selection has been made 20 After reviewing the program summary select to save changes and return to to cancel and lose all changes Main Menu or Editing Programs EDIT To edit a program select from the Main Menu 2 Use the up and down arrow keys Figure 6 1 to navigate through the list of existing programs Highlight the program to be edited 3 When the desired program is highlighted select Program Edit Program 4 To rename the program select Name from the Program Steps Summary Screen A keypad as shown in Figure 6 2 will appear onscreen Delete the current name of the www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 30 10 11 Clear All rogram by selecting Type in the desired name of the program and select to save MN Edit Step To edit a process highlight the desired step number and select The Editing Program Steps Screen as shown in Figure 6 3 will be displayed Change the appropriate settings by following Steps 8 13 of the Creating Programs section Once all desired steps have been edited select to return to the previous screen To insert a process prior to
8. Unload Drawers Ensure that proper reagents are loaded in the Stainer at proper locations for the protocols that you will be running Now you can load the slides in a Slide Rack open the Load Drawer install the rack in the drawer and close the drawer The program Selection Screen will automatically appear on the Display Select the desired program and press RUN Once the rack has been picked up from the Load Drawer you can add another rack for processing When the staining is completed the Stainer will put the processed rack in the selected Unload Drawer Open the Unload Drawer with the rack remove the rack and close the drawer Failure to remove the processed rack in a timely manner may delay the processing of the other racks Shutdown Procedure 1 2 3 Once all racks are processed for the day remove them all from the Unload Drawers Shut off the water supply You can remove the reagent vessels and store them in a refrigerator or you can leave them in the Stainer You can place the lids over the reagent vessels to minimize the evaporation or you can leave the vessels uncovered As you prefer Make sure that you www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 15 load the refreshed reagents next time you use the stainer and remove all the lids before starting the next processing run 4 You can leave the Stainer ON overnight or you can choose to power it down Welcome Screen After powering up the HistoPro 303
9. an existing step navigate through the list of programmed steps using the up and down arrow keys Highlight the step that occurs after the one to be Insert New added and select Step to add and edit the new step Once all desired settings have been programmed select to return to the previous screen To add a process after a programmed step navigate through the list of programmed steps using the up and down arrow keys Highlight the step that occurs before the one to be Add New added and select 19 to add and edit the new step all the desired settings have been programmed select to return to previous screen To delete a process navigate through the list of programmed steps using the up and down arrow keys Highlight the step to be deleted and select Step to be asked to confirm the permanent step removal After making all the necessary changes review the Program Steps Summary Screen to ensure the entire program definition is correct Select to save changes and return to Main Menu Select to discard changes and return to Main Menu You will receive a discard changes confirmation message to verify the request to exit without saving Note Programs numbered 41 50 cannot be edited using the HistoPro 3030 stainer software They can only be viewed in the editor and not modified These programs are created and edited by using the HistoProgramEditor Windows application software See the section on Importing and Exportin
10. and represent the one load station and two unload stations Finally each station that is occupied by a rack 1s filled with a color that is unique to each rack in progress www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 36 This can help to visualize which stations are occupied and follow each rack as they move from station to station according to the program To view detailed information on a rack of slides currently being processed hold your stylus on the icon of the station in question for a couple of seconds If that station is currently holds a rack of slides a popup window will momentarily appear at the top of the screen This Rack Status Popup Display shown in Figure 7 4 displays five items of information The STAION NUMBER appears at the left of this window while the PROGRAM NUMBER and the STEP NUMBER appear at the right and the RACK NUMBER and the TIME REMAINING in this step appear in he center Rack Number Program Number Remaining Time 00 00 41 Step Number Station Status Figure 7 4 Rack Status Popup Display Unloading Racks When a rack has completed processing as determined by the steps in the selected program it will be dropped off in the designated unload station Station 21 or Station 22 The stainer will beep once and the LED on the front of the unload drawer will begin flashing In addition the Stainer Running Screen will show the unload station icon as flashing This is the indicat
11. the stainer the Cancel Racks button will appear in addition to the Cancel One Rack button 5 Select pr to immediately cancel all processing racks and re initialize the stainer to the startup state 6 Select cancer to view the Cancel Rack Selection Screen as shown in Figure 7 6 7 If applicable use the plus and minus buttons to cycle through each currently running rack so that the desired rack number is displayed The program number and station number for each rack appears in order for you to verify your selection www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 38 8 Based on which unload stations are not occupied select whether to move the cancelled Unload 21 Unload 22 rack to unload station or or select to manually remove the rack from the stainer 9 Select the ee button to complete the cancellation or the ww button to return to the previous screen 10 If you chose to manually remove the rack you must open the stainer and remove the cancelled rack from its station when prompted and before resuming processing None Figure 7 6 Cancel Rack Selection Screen www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 39 Running Multiple Racks The HistoPro 3030 stainer is designed to run multiple slide racks at the same time and optimizes a schedule to process all running racks as quickly as possible The following guidelines apply when run
12. to select one of those options at all times Please refer to the section of documentation for each feature for more information on the user interface screens and options offered to the end user Utility Menu Time Settings Agitation Setup Export Programs Temperature Flow Adjustments Reserved 2 Import Programs Change Password Figure 5 4 Utility Main Menu Screen Time Settings To change the current date and time for stainer display and logging purposes edit the time and date fields as shown in Figure 5 5 The plus and minus sign buttons that appear above and below the hours minutes month day and year fields allow you to modify the values Please be aware that the time value is displayed in 24 hour format Select the Accept button to save the settings or Cancel to ignore the changes www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 18 Figure 5 5 Time Settings Screen Agitation Setup To change the number of dips performed during agitation operations edit the number as shown in Figure 5 6 The plus and minus buttons that appear above and below the dips per station number can be selected to modify the desired value Select the Accept button to save the setting or Cancel to ignore the changes Figure 5 6 Agitation Setup Screen www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 19 Temperature and Flow Adjustments To change the temperature and flow control adjustme
13. 0 the following screens are displayed in order Please wait until all the initialization steps have been completed before attempting to use the stainer HMS730 Sta in er Version 1 0 System Initialization in progress Initializing Software Figure 5 1 Startup and Stainer Initialization Manually inspect the stainer to ensure that all stations are free from slide carriers then select to continue as shown in Figure 5 2 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 16 Figure 5 2 Welcome Confirmation Screen The following Main Menu screen will be displayed Figure 5 3 Proper operation of the system can by achieved by making selections from this and the software screens that follow To make a selection gently press upon the desired button with a stylus 05 33 43 AM Saturday Aug 12 2006 To Run a Program UTILITIES Open Load drawer add Slides Close Load drawer and select Program SERVICE Figure 5 3 Stainer Software Main Menu www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 17 Changing Default Settings The HistoPro 3030 software offers a user friendly Operator Interface to set up and run the stainer To select different functions locate the necessary button on the LCD screen To make a selection gently press upon the button with your finger or a touch screen stylus stylus not provided The display prompts the user about what options are available and how
14. IMITATION THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE TBS SHALL NOT IN ANY CIRCUMSTANCE BE LIABLE FOR ANY SPECIAL INDIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES Contact our Product Service Department for more information regarding your warranty Product Service Department Triangle Biomedical Sciences Inc LLC 3014 Croasdaile Drive Durham NC 27705 Phone 919 384 9393 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 4 Definition of Various Symbols Attention Consult accompanying documents This symbol is used to direct the user to refer to the documents for additional information regarding the system use or description Caution Electric Shock Hazard Disconnect Line Cord before Servicing Refer h Servicing to Qualified Service Personnel A Hot Surface Surfaces may be very hot to the touch www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 5 Product Description The HistoPro 3030 continuous load slide stainer offers flexibility and programmability for routine and special stains of samples mounted on glass slides This bench top unit contains 30 stations Specific functions of some of these stations are as follows Station Number How Many Function 30 1 Load Station 21 22 2 Unload Stations 5 6 7 8 4 Rinse Stations Manual Flow Control with a common inlet 9 1 Rinse Station Manual Flow Control with an independent inlet 10 20 2 Hea
15. NNOVATIONS FOR SCIENCE Page 41 Charcoal Filter behind top left bezel Figure 8 1 Charcoal Filter behind top left bezel www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 42 Service Mode The HistoPro 3030 stainer has a Service Menu as pictured in Figure 8 2 to allow trained support technicians to perform maintenance in the field Improper use of commands and the functionality available through this mode can damage the Stainer and void the warranty DO NOT USE SERVICE MODE if you do not have proper training to operate various commands available to exercise various functions Please contact your support technician if you are experiencing software or instrument problems with the HistoPro 3030 The following screen is available to test and service the stainer and can only be accessed from the Main Menu after entering the correct password Slave Control Program Test History Log Information Total Racks Processed Figure 8 2 Service Menu Screen www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 43 CHAPTER 9 SPARE PARTS AND ACCESSORIES Item Description Part No Reagent Vessels and Lids 8 each per Pack A12540 Slide Rack 1 per Pack A12541 Operator Manual 12537 Service Manual A12636 Water Inlet Hose with In Line Filter A12542 Heater Station Vessel A12543 Rinse Station vessel without A12544 Charcoal Filter A12637 3 Position Vessel Carrier 12545 4 Position V
16. Shutdown Procedure c 15 Welcome Screen 16 Chaneme Default Settings AN 18 Te Eb HEB SP ce 6 iier ater crocuses A ET ad aae vase CES uf 18 Azitain Setup eM 19 Temperature and Flow Adjustments pp 20 DSEGULIEV PASS WTO S os E E E EE E ENE A Sys 20 Chapter 3 BrogEamTm1 id 23 Creating Programs OO UL LL 23 sodes Geddes vient a Reste icit LEE 30 CU 32 Copying Programs Ede ERR OON 22 Importing and Exporting Programs AS 32 Chapter 6 Running Programs 34 Loading 34 Run Lone 36 Enloadme RACKS oo oO dtd utendo oem qv dtes aetas da deti c NUM 37 Cancelling ROSE 38 Runne Multiple 40 Chapter 7 Maintenance 2 1 eene e deside E E T S 41 Routine Maintenance P 41 Procedures xoc ador resi etta Mets dits ca beiden D Oe e nes niei ee 41 Charcoal Filter Replacement r au aep aea e Rates T at 41 Service ES 43 Chapter 8 Spare Parts and Accessories iscriere oreeson oia a i ananena 44 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 2 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 3 CHAPTER 2 INTRODUCTION Warranty Information Triangle Biomedical Sciences Inc TBS HistoPro 3030 products are warranted against defects in materials and workmanship under their p
17. essel Carrier 12547 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 44
18. g Programs for additional information www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 31 Deleting Programs EDIT To delete a program select from the Main Menu Navigate through the list of programs using the up and down 0 arrow keys until the program to be deleted is highlighted Select and confirm the operation by selecting Yes when prompted 2 4 Select to return to Main Menu Copying Programs EDIT 1 To copy a program select from the Main Menu 2 Navigate through the list of programs using the up and down arrow keys until the program to be copied is highlighted 3 Select Copy ea 4 Navigate through the list of programs using the up and down arrow keys until the desired blank slot to copy the program to is highlighted 5 Select mem to perform the copy operation 6 keypad as shown in Figure 6 2 will automatically be displayed Delete the name of the T f Clear All original program by selecting and name the duplicated program using the onscreen keypad After entering the desired name select to save 7 A summary of the copied program with its newly programmed name will be displayed 8 Select to save changes and return to Main Menu Importing and Exporting Programs The Utilities menu contains two buttons that allow the import and export of programs to and from the stainer see Figure 5 4 Please note the import and export operations are advanced fea
19. he power switch in the back of the unit Now the Stainer can be turned on by operating the switch located in the front on the lower right hand side ON ee Ge Ee www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 14 CHAPTER 5 OPERATING INSTRUCTIONS Operating the HistoPro 3030 Stainer Set Up Procedure 1 2 3 Load appropriate reagents in the reagent vessels at stations 1 5 11 19 and 23 29 Load Xylene Xylene substitute or water as appropriate at unload stations 21 and 22 Make an appropriate drain connection from the drain fitting in the rear of the unit to a sink Make sure you follow your local regulations for draining the various chemicals Ensure that the drain line is not kinked and is at a level below the drain fitting from the system Pour about 2 liters of water in one of the rinse stations and ensure that the water drains from the unit without any restrictions Connect water supply in the rear of the unit for rinse stations Turn on the water supply You are ready to use the Stainer Operating Procedure 1 Turn ON the Stainer by operating power switch located in the front on lower right hand side Once the Stainer initializes the Mechanisms follow the procedures described in the later sections of this manual to set up various Staining Protocols Remove all the lids from the reagent vessels Remove all slide racks if any are present from the Stainer Do not forget to check the
20. heir programs It is recommended that each user analyze the set of programs that will be run and the desired throughput to determine the maximize number of racks to run at one time www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 40 CHAPTER 8 MAINTENANCE Routine Maintenance DO NOT use excessive amounts of solvent for cleaning DO NOT use the stainer for purposes other than its designed function DO practice good housekeeping and maintain the stainer in a clean condition DO switch off the stainer before removing plug DO ensure that replacement fuses are of the correct specification Cleaning Procedures HistoPro 3030 system is designed to properly function under recommended settings Please use the following instructions and cleaning procedures carefully e Disconnect line cord from stainer when cleaning The stainer should always be disconnected from the mains power supply when cleaning particularly if flammable solvents are used e Use only minimal quantities of solvent on an absorbent cloth We recommend using a xylene substitute for cleaning the stainer Charcoal Filter Replacement We recommend that you replace the charcoal filter every three months or at more appropriate interval based on your usage of the system The charcoal filter is located behind the top left bezel Pull the bezel straight out in front and you will be able to access the charcoal filter as shown Figure 8 1 www trianglebiomedical com I
21. ion to remove the rack from the stainer as soon as possible It is necessary to remove a rack from each unload station quickly in order to allow subsequent racks to be processed successfully If an unload station is occupied when a rack is scheduled to be sent to that station the stainer will start beeping and pause all operations until there is user intervention Once the drawer is cleared of the rack and the unload drawer is closed the beeping and flashing will stop and processing will automatically continue www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 37 Cancelling Racks While the stainer is processing a rack there are situations when a rack must be cancelled In addition sometimes the operator needs to temporarily stop the stainer to evaluate the state of the running racks and determine if any racks need to be cancelled and removed To cancel one or more racks from processing the following steps must be followed 1 Select from the Stainer Running Screen to pause the HistoPro 3030 2 The stainer will continue its current motion if necessary before going into a paused state The instrument has been PAUSED and will only complete its current motion Select RESUME or CANCEL RACK to continue Figure 7 5 Cancel Rack Option Screen 3 If you change your mind select to return to the previous screen and continue processing racks 4 When there is more than one rack currently being processed by
22. ipe only Figure 2 3 HistoPro 3030 Rear View www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 10 CHAPTER 3 SPECIFICATIONS Electrical Characteristics Power Input Voltage AC 100 115 volts or 230 volts Factory set for either 100 115 volts or 230 volts operation not user changeable 50 or 60 Hz Current 10 Amps max for 120 volts input 8 Amps max RMS for 230 volts input Power 1200 watts max Fuse Rating 10 Amp x 250 volts Time Delay Slo Blo fuse for 115 volts units 8 Amp x 250 volts Time Delay Slo Blo fuse for 230 volts units Display Programmability Display Active matrix TFT 6 5 display Screen Resistive touch pad Number of programs 50 Number of steps program 0 Physical Characteristics Dimensions Width 34 85 cm Depth 25 63 cm Height 18 48 cm Weight Actual Weight 65 Ibs 30 kg Capacity Number of Stations 30 Number of Rinse Stations 5 user selectable Rinse Water Flow Control Separate manual control for each Rinse Station Number of Rinse Water Inlets 2 one inlet feeds four stations the second inlet feeds one station Number of Load Station 1 Number of Unload Stations 2 Reagent Container Volume 350 ml Slide Carrier Capacity 30 slides Drying Station Temp Range 40 70 C www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 11 Environmental Conditions All HistoPro 3030 products are laboratory instruments and should be used in Medical Histo Patholog
23. may damage the stainer and void the warranty Power Sources This product should be operated from the type of power source indicated on the marking label and in the instructions Grounding This product is equipped with a grounded three wire plug Power Cord Protection Power supply cords should be routed so that they are not likely to be walked on or pinched by items placed upon or against them Pay particular attention to cords at plugs receptacles and the point where they exit the stainer Overloading Do not overload wall outlets and extension cords as this can result in a risk of fire or electric shock Servicing Do not attempt to service this stainer yourself as opening or removing covers may expose you to dangerous voltage or to other hazards Refer all servicing needs to qualified service personnel Replacement Parts When replacement parts are required be sure the service technician uses replacement parts specified by the manufacturer Unauthorized substitutions may result in fire electric shock or other hazards www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 13 Inspection e The HistoPro 3030 is carefully quality controlled prior to shipping The highest quality packaging materials have been used to ensure that the equipment is well protected during shipping Please follow these inspection instructions carefully e Carefully inspect the outer carton for any visible damage If any damage is no
24. ning multiple racks in the stainer simultaneously e You must wait until a rack is picked up from the Load Station and dropped off in the station designated by the first step of the selected program before inserting a new rack into the Load Station The Load Station indicator light on the front of the stainer will stop flashing green when it is ready to accept a new rack When a rack is already running and a new slide rack is inserted into the Load Station the software must determine the optimal schedule to run all racks simultaneously This processing may take several seconds so a Please Wait message will appear on the screen Please note that the stainer will finish any current motions before the scheduling calculation begins e When a new slide rack is inserted and the stainer is in the middle of processing an existing rack in a station marked as critical Step Criticalityz Yes the scheduling of the new rack will be delayed Once the existing rack has exited the critical station the new rack will be scheduled as usual e Running multiple racks at the same time is more efficient than waiting until each rack is completed before starting the next one However there are situations when there is insufficient time to process additional racks and not adversely affect the racks already in progress In those situations the new rack may not start processing immediately or it may cause the existing set of racks to take longer to complete each of t
25. ns off the password entry screen While viewing the Utility menu screen the Change Password button can be selected to modify the www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 20 current password The password can be set to a maximum of 25 characters and is not case sensitive Once the password is set the password entry screen as shown in Figure 5 8 appears to allow password entry The Edit Password Text button can be selected to view the onscreen keyboard for character entry Change Password Figure 5 8 Enter Password Screen After selecting the button the password entered will be validated and access to the requested screen will be allowed or denied as appropriate The change password screen 15 shown in Figure 5 9 and requires that you enter the current password to change the password to a new value www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 21 Figure 5 9 Change Password Screen www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 22 CHAPTER 6 PROGRAMMING The HistoPro 3030 slide stainer offers flexibility and programmability for routine and special stains The system can store up to 50 programs numbered 1 50 Note Programs 41 50 are reserved for servicing purposes These programs may not be edited by unauthorized personnel Please refer to the operators manual for the HistoProgramEditor software utility for more information on this feature Creating Pr
26. nt settings edit the values as shown in Figure 5 7 The temperature adjustment controls appear at the top with a separate setting for each temperature station Station 10 and Station 20 The value can be set in the range from negative to positive five using the plus and minus buttons The Test area for each temperature station can be used to turn on and off the heater for each station and physically test the stainer for accuracy The adjustment value is added to the temperature requested by each program that utilizes station 10 or 20 during processing The default value for each adjustment setting is zero The flow control adjustment works in a similar way except it accepts a positive or negative percent value from 10 to 10 The setting applies only to the variable flow control station which is Station 9 The test controls can be used to turn on and off the flow valve at a standard value and physically test the stainer for accuracy The adjustment value is used during rack processing to add or subtract a percentage of the program specified flow control for Station 9 Figure 5 7 Temperature and Flow Adjustment Screen Security and Passwords Operation of the stainer can be limited by implementing a password security scheme With the password featured turned on a valid password will be required to access the following screens from the Main Menu e Edit e Utility e Service By default the password is set to 0000 which tur
27. ograms EDIT 1 Select to display the following program listing screen No 1 Sample Program Figure 6 1 Select Program Screen 2 New programs are created by editing blank programs Blank programs are typically labeled with hyphens as shown in Figure 6 1 Use the up and down arrow keys to navigate through the list of existing programs numbered 1 40 3 Highlight the desired empty program slot and select Eat Program to create a new program www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 23 Edit Program 4 To name the program select Name as shown in Figure 6 7 Program Steps Summary Screen An onscreen keypad as shown in Figure 6 2 will appear New Program oeae gt 1 SPACE 4 Figure 6 2 Onscreen Keypad 5 Enter the name of the program Use the and buttons to switch between the uppercase keypad and the lowercase keypad while entering the desired program name 6 When the desired program name has been entered select to save Add New 7 To add the first step of the program select Step The following screen will appear with the step number clearly labeled in the heading as shown in Figure 6 3 www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 24 Current Step Number New Program Step 1 Station Agitation O YES NO Time at Station m s Criticality
28. rescribed use and operating conditions for a period of twelve months from the date of shipment TBS will repair or replace products that prove to be defective within the warranty period without charge when shipped prepaid to the TBS office If the product is not registered by filling out and sending the warranty card to Triangle Biomedical Sciences Inc it will be warranted for a period of twelve months from its manufacturing date Warranty Exceptions Consumables bulbs fuses and other items of an expendable nature are not covered under this agreement User s failure to provide ample electrical power at constant voltage consistent with the specifications of the product Scratches dents and similar surface finish damage during normal use of the system are not covered under this agreement Misuse and unauthorized repairs or alterations are not covered under this agreement Acts of nature such as lightning strikes and floods are not covered and should be covered under owner s separate insurance policy All TBS products are sold on the condition that they be used and disposed of only within the scope of currently recognized critical standards related to human health and the physical environment EXCEPT FOR THE WARRANTY ABOVE TBS MAKES NO OTHER WARRANTY OF ANY KIND WITH REGARD TO ITS PRODUCTS WHETHER EXPRESS ARISING BY OPERATION OF LAW OR IMPLIED BY COURSE OF DEALING USAGE OF TRADE OR OTHERWISE INCLUDING WITHOUT L
29. rogrammed time can be incremented or decremented by pressing the t and __ buttons until the desired time number is displayed The duration time is displayed as minutes seconds Note Actual duration time may exceed programmed duration time when multiple programs are run simultaneously See Running Multiple Racks for more information Note The time can be incremented or decremented more quickly by selecting and holding the stylus on the plus or minus button and released when the desired value is reached Carrier Pickup Speed refers to the speed at which the carrier is removed from the reagent containing station vessel Desired carrier pick up speed settings may be programmed by appropriately selecting the Normal or Fast radio button that appears under the corresponding headings Agitation is a feature that relies on the carrier to move the slide rack in an up and down motion to ensure even stain distribution based on the agitation setting Agitation can be turned on or off for a station by selected the Yes or No radio button Criticality is a feature that ensures a rack is not left submerged in a station for longer than the specified duration time If the step is flagged as critical by selecting the Yes radio button the duration will not be extended to accommodate multiple rack processing The temperature can be programmed under Temperature deg C for processes that take place at Heating Stations
30. stoPro 3030 station while displaying monitoring information and providing a stop button The top of the display shows a line by line log of the actions taken by the stainer to process racks in each station in real time This information can be used to monitor the previous steps taken for a rack that is processing and shows rack movement heating station temperature and rinse station flow rate In addition the log lists when a scheduled rack will begin processing along with any notification or error conditions that occur during stainer operation Rack 1 1 lt lt SCHEDULED Start 10 07 30 Stop 10 13 11 10 07 33 Rack 1 Program 1 START HistoPro 10 07 33 Rack 1 Picking up from Load Station and moving to station 1 for dropoff 10 07 34 Picking up at 10 07 34 AM Rack 1 Dropping 3030 Log Station 1 is Occupied Figure 7 3 Stainer Running Screen The stations numbered 1 to 30 appear as they do in the physical stainer with the addition of color coding to indicate the state and purpose of each station Stations 5 to 9 are highlighted in light blue to indicate the are the rinse stations with Station 9 offering variable flow control Stations 10 and 20 are highlighted in orange to signify they are the heating stations In addition the current temperature in degrees Celsius is listed for each of these stations Stations 30 21 and 22 are green in color
31. the extreme right feeds water to stations 5 67 and 8 simultaneously When a slide rack is programmed to be in any of these stations the water will flow through all the stations The second inlet to the left of this common inlet feeds only to station 9 This allows you to use DI water distilled water or any other specialized buffer to be fed in only one station while using tap water for all other rinses You can use standard 1 1 4 drain pipe from your local hardware store to provide proper drainage of the rinse water and any spills Please do ensure that the drain water flows freely to the sink and the drain plumbing is free of kinks and is always at a level below the stainer bottom www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 7 Bezel Door for Charcoal Filter Computer and Display Load Drawer Station 30 Unload Drawer 1 Unload Drawer 2 Station 21 Station 22 Figure 2 1 HistoPro 3030 Front View www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 8 Rinse Station with an Heating Station 1 Independent Water Inlet Heating Station 2 4 Rinse Stations With Manual Flow Control Up Down Mechanism Flow Control Valves In Out Mechanism Pick Up Arm Figure 2 2 HistoPro 3030 Functional Areas www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 9 Rinse Water Inlet Feeds Stations 5 8 Drain Connection Rinse Water inlet Accepts 1 1 4 PVC Feeds Station 9 Drain P
32. ticed contact the shipping carrier and file a damage report before unpacking the stainer Failure to report visible damage may forfeit any claims for internal damages e Inspect the system for any visible damage If any shipping damage is visible retain all packing material intact with the stainer and file a claim with the final carrier Discarding any packaging materials prior to the carrier s inspection may void any shipper liability Components Checklist Each HistoPro 3030 stainer comes with the following components If an item is missing please contact Triangle Biomedical Sciences Inc Customer Service immediately at 919 384 9393 Item Quantity HistoPro 3030 Stainer 1 Power cord 1 Operator s Manual with Warranty Registration Card 1 Reagent Containers 23 Reagent Container Lids 23 Drying vessels for heater station Slide Rack Computer to be installed to the Stainer by the user Charcoal Filter Pre installed NN Installation Procedures Follow the instructions provided as SAFEGUARDS and INSPECTION procedures Remove the HistoPro 3030 stainer from the wooden crate and place on the countertop Remove all packaging materials Ensure that all the tie wraps have been removed and the mechanism is free to move Install the Computer Connect the power cord to the stainer and plug the power cord into an electrical outlet The software necessary to run the HistoPro 3030 stainer comes pre installed Turn t
33. ting Stations Tt should be noted that all the heating stations and rinse stations can be configured as reagent stations The stations are numbered from left to right and from the back row to the front row Stations 1 10 are located in the back row stations 11 20 are in the middle row and 21 30 are located in the front row This manual covers information about initial set up programming test protocols and slide staining processing operations only The details of the functions to be performed by the qualified service technicians are provided in the Service Manual Part 12536 The operator interface is quite intuitive however several sample tasks are described to minimize the learning curve Due to the continuous improvement of the product software it is possible that some of the newer product features are not described in this version of the manual or they may operate differently If you have any questions or comments about the information provided in this manual please contact our Product Service Department at 919 384 9393 or send an e mail to tbs trianglebiomedical com www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 6 Functional Description of Parts Refer to Figure 2 1 HistoPro 3030 Front View Figure 2 2 HistoPro 3030 Functional Areas and Figure 2 3 HistoPro 3030 Rear View The HistoPro 3030 H amp E Slide Stainer has the following functional areas Reagent Stations Rinse Stations Load Stations
34. tures and are recommended only for the person who is the HistoPro 3030 administrator www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 32 Export Programs Select to output the current set of programs from the stainer to an attached USB key An error message will appear if a valid USB Key is not plugged into the stainer The purpose of exporting programs from the HistoPro 3030 is to edit them on a Windows PC computer and share programs with multiple units Please refer to the instructions for the HistoProgramEditor external program for details on how to edit programs on a PC and transfer those programs from a computer to one or more HistoPro 3030 stainers After editing the programs on a PC using the HistoProgramEditor attach the USB Key to the stainer and select Import Programs The program definition files will automatically be imported to the HistoPro 3030 and will replace your current set of programs Please be cautious when importing programs since any changes to the current set of stainer programs since the last export will be lost upon import www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 33 CHAPTER 7 RUNNING PROGRAMS The primary purpose of the HistoPro 3030 stainer is to process slide racks using a pre defined program Each program follows a series of steps to move racks from station to station according to schedule and station processing parameters Loading Racks 1 2 3
35. y laboratory environments with the following conditions Ambient Temperature 62 to 90 F 17 32 C Relative Humidity 20 85 non condensing Ambient Pressure 29 31 74 79 mm of Hg Installation Category In reference to Installation category as defined in UL 61010 1 standard HistoPro 3030 products are categorized as Category II systems Pollution Degree In reference to Pollution Degree as defined in UL 61010 1 standard HistoPro 3030 products are categorized as Pollution Degree 2 systems www trianglebiomedical com INNOVATIONS FOR SCIENCE Page 12 CHAPTER 4 INSTALLATION INSTRUCTIONS Safeguards Before doing anything read this Please read all the instructions and retain for future reference Follow all warnings and instructions marked on the stainer 1 2 3 10 11 15 Read Instructions All the safety and operating instructions should be read before the stainer is operated Retain Instructions The safety and operating instructions should be retained for future reference Read Warnings All warnings on the stainer and in the operating manual should be followed Follow Instructions All operating and use instructions should be followed Attachments Do not use any attachments not recommended by the product manufacturer as they may cause hazards damage the stainer and void the warranty Accessories Do not use any accessories not recommended by the product manufacturer as they
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