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1050 Series of HPLC Modules Service Handbook

1. Keyboard Figure 11 Block Diagram of Keyboard Electronics S KDI lt x O ia BUZZER rozs wW Z aoa amp DRIVER BUZZER SS m N m T CY yO O J16 Vee TO DCB PY YY BUZZER gt _ LED DATA DRIVER BUS Hq e INTERFACE lt lt J AD A KEY WR a a t lt lt lt MATRIX CTRL CLK lt lt LINES gt RESET lt gt cs lt BUSY lt ses ME Vec gt Vss gt GND gt J17 Vv aw ANODE CPU LATCH BUSY WR DRIVER VACUUM FLD cs GRID 5 5V Ld 5 DC DC z WFD CONVERTER 5 604 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 6 Figure 12 VWD Electronic Information Pre Amplifier Boards Pre Amplifier Boards Repair Level Board Part Numbers for Pre Amplifiers Item Part Number Pre Amplifier Board Sample 79853 66507 Pre Amplifier Board Reference 79853 66508 Sensor Sample 79853 61109 Sensor Reference 79853 61110 The light absorbtion from the deuterium lamp flow cell is detected by the sample and the reference photodiode Its current is then amplified by the pre amplifiers The signal then is routed to the DCB The wires from the photodiodes are soldered onto the board Photodiode Assemblies Ws
2. new lens assembly r OQ na new mirror 1 assembly new beam splitter aN assembly o a m new standard flow cell Service Handbook for 1050 Series of HPLC Modules 11 2001 707 NOTE NOTE VWD Enhanced Optical Unit Information Support Considerations Support Considerations Prefix Change The enhanced Optical Unit D version was introduced in production units in June 1995 Since the detector appears to look the same as before a prefix change was made All units with prefix 3522 J 04305 and above have the new optical installed Some units with a prefix lower than 3522 J 04305 have been installed on customer sites prior to the official shipments Identification Following identifications for the enhanced D version are available e Prefix and serial number 3522 J 04305 and above rear of instrument e firmware revision 4 31 press CTRL 12 ENTER ENTER DOWN e label on the optical unit ENHANCED ILLUMINATION SYSTEM e handle of new reference slit looking out of the optical s cover plate see Figure 266 on page 715 Compatibility Matrix Due to a redesign several components are usable in the enhanced D version on
3. Service Handbook for 1050 Series of HPLC Modules 11 2001 689 VWD Parts Information Optical Unit C Optical Unit C Inner Parts Bottom Table 5 Optical Unit C Inner Parts Bottom Item Description Part Nmber Item Description Part Nmber Optical Unit comple assembly 79853 60015 7 Filter Assembly see page 692 Plate Optical Base 79853 04109 7 Position Sensor Assembly Filter 79853 61107 1 Optical Body N A 8 Cable Assembly Sample 79853 61607 2 Plate Sample Sensor 79853 04110 9 Cable Assembly Reference 79853 61608 3 Position Sensor Assembly 79853 61106 Spacer Optical metal foot 79853 24701 Grating 4 Plate Reference Sensor 79853 04111 Insulator Optical rubber foot 79853 85401 5 Spring 1 Grating 79853 29102 Foot Kit Optical 79853 22005 6 Grating Assembly see page 691 Figure 6 Optical Unit C Inner Parts Bottom 31 690 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 6 Figure 7 VWD Parts Information Grating Assembly Grating Assembly Grating Assembly Description Part number 1 Grating 79853 64605 Hex Screw M3 14 mm lg 79853 29103 79853 64606 79853 61106 2 Spring 2 Grating 3 Gasket 4 Grating Motor Assembly
4. Sample Reference Service Handbook for 1050 Series of HPLC Modules 11 2001 605 Table 7 NOTE Figure 13 VWD Electronic Information Power Supply Connection Board PSC Power Supply Connection Board PSC Repair Level Board Part Numbers for PSC Board Item Part Number PSC Board for DCB 66511 79853 66512 PSC Board for DCB 66506 OBSOLETE 79853 66509 OBSOLETE If the DCB board is replaced by 79853 66511 version the PSC board MUST be changed to 79853 66512 This board connects the Power Supply DPS A with the DCB and the Deuterium Lamp Assembly The wires of the lamp cable are soldered in Board Layout PSC O PSC BOARD O J20 GND HEATER ANODE 606 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 8 Parallel Interface Microprocessor Memories Firmware Description GPIB Address Setting VWD Electronic Information GPIB Communication Interface GPIB Communication Interface Repair Level Board Firmware Part Numbers for GPIB Interface Board Item Part Number GPIB Board with cable 79853 68711 GPIB Cable to DCB Board Firmware ROM GPIB 79853 13004 Dual direction transceiver for data bus between master and slave CPU s 8 bit aux code from DCB to GPIB board 3 bit control code from GPIB board to DCB Single chip microprocessor with 1 Mbyte address capability 512 byte in
5. NS CTRL _ H THH ul Service Handbook for 1050 Series of HPLC Modules 11 2001 569 NOTE Figure 4 VWD Hardware Information Leak Sensor Assembly Leak Sensor Assembly A drain is located at the bottom of the cell compartment and can be led to the waste container or the 1050 waste handling system the VWD has to stand on top of another module when the leak interface is not used A leak sensor is located behind the front panel assembly Figure 209 Solvent would cool the leak sensor self heating thermistor The resulting change in resistance measured by comparators on the Detector Controller Board DCB would generate an error message and switch the deuterium lamp OFF In the 1050 System remote mode the leak message will turn off the pump Leak Sensor Assembly 4 DOK Lever N Thermistor Leak out Leak sensor assy 570 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Fan Assemblies Fan Assemblies The instrument is equipped with two fans On the 79853C VWD the fans are of different type HIGH type this fan is located close to the lamp housing and runs with a higher speed LOW type this fan is located at the rear under the GPIB interface and runs with a lower spe
6. Service Handbook for 1050 Series of HPLC Modules 11 2001 623 20 0th CALIB Table 5 VWD Diagnostic amp Troubleshooting Information Service Control Functions Zero Order Calibration This control function is used for the electrical calibration of the zero order beam of the monochrometer The step number corresponds to the number in 31 SET WL PARAM The grating is driven by the stepper motor through steps 150 to 250 searching for a maximum The step number with the maximum voltage is displayed and if accepted written into the EEROM The intensity of the zero order light is reduced by inserting a filter in the calibration position automatically Prior to the use of this function the grating assembly should be pre aligned on the zero order light using control function 40 0TH TEST OFF Control Function Zero Order Calibration Key Operation Display Description 20 0th CALIB Select function ENTER nn step CHANGE display current parameter or change to 200 ENTER or to continue calibration or CLEAR to abort wait appr 20 seconds nn step x xxxV starts the calibration scan from 150 step to 250step No key entry is allowed here Possible error message at this point may be CALIB FAILURE Indicates that the lamp is OFF DATA OVERFLOW Indicates that too much light reaches the sample photo diode Reduce light and insert paper between cell and sample diode 624 Service Handbook for 1050 Series
7. VWD General Information This chapter provides general information about the 1050 Variable Wavelength Detectors VWD General Information This chapter gives general information on e about this detector e repair policy e specifications 560 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD General Information About the Detector About the Detector The 1050 Variable Wavelength Detector VWD is a detector of the modular type liquid chromatograph 1050 Series This is a standalone grating photodiode type general purpose detector The performance and features match the requirements of the routine analysis and QC QA analysis The 1050 VWD is a standard size detector of 1050 modular type LC series and can be build up with other LC modules such as pump and automatic sampler Since it is standalone type it can be also used as an ordinary LC detector It has a functional keyboard and 16 character fluorescent display which provides you easy operation Versions vs Support Periods EOS 79853A e The 79853A VWD was shipped between May 1988 and January 1992 The support with parts ended November 1 2000 79853C e The 79853C VWD replaced the model 79853A VWD in January 1992 The end of support EOS will be August 1 2006 with all 1050 series HPLC modules e The 79853C VWD got a redesigned optical unit D enhanced optical see VWD Enhanced Optical Unit Information on page 705 that replaced
8. C version In case of replacing a C optical unit with an enhanced D optical unit the new standard flow cell is required Support of Previous Optical Units The parts for the C optical unit will continue to be available as a repair part as long as the 79853C is supported 08 2006 In case the complete C optical must be replaced use the D upgrade mentioned on Part Numbers for Enhanced D Optical Unit on page 709 706 Service Handbook for 1050 Series of HPLC Modules 11 2001 Figure 1 new entrance slit assembly new reference slit VWD Enhanced Optical Unit Information Introduction Introduction To overcome wander problems due to temperature variations of the lab environment the optical unit of the 79853C Variable Wavelength Detector VWD has been modified Following hardware modifications were implemented in June 1995 different coupling of the lamp housing lens between lamp housing and optical casting area around mirror M1 and M2 has been redesigned to eliminate one mirror result is a mirror 1 assembly with a plane mirror redesigned entrance slit holder Slits are changeable standard test beam splitter assembly no longer vertically adjustable reference slit assembly redesigned for better optimization new standard flow cell with different aperture material and different inlet capillary Optical Path of Enhanced Optical Unit
9. with STD to improve temperature flow cell D in June 1995 stability see VWD Enhanced Optical Unit Information on page 705 730 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 2 VWD Additional Information DCB ROM Firmware Revisions DCB ROM Firmware Revisions DCB ROM Firmware Revisions Revision Major Changes Comments 4 08 4 09 PHOENIX problem won t stop when running with A D Concerter for example ABORT 421 CTRL 45 COMPENSATE bug fix calibrated wavelength deviation might exceed the specification CTRL 22 HOLMIUMCHECK for GLP reasons added 4 22 New algorithm for CTRL 45 COMPENSATE and CTRL 22 HOLMIUMCHECK 4 23 Removed bug Incorrect wavelength setting during stepper motor intialisation bewteen 536 4 nm and 600 nm Used for first internal Waldbronn Avondale units and some demo units Not released Used for internal Waldbronn Avondale tests Released for 79853C in January 1992 For replacements use part number 79853 13005 4 24 NEEDS GPIB ROM version 0 20 or above 79853 13004 Released for 79853C in March 1992 Started with serial number 3152300489 For replacements use part number 79853 13005 4 24 NEEDS GPIB ROM version 0 20 or above 79853 13004 Released for 79853C in April 1992 Started with serial number 3217J00603 For replacements use part number 79853 13005 4 24 NEEDS GPIB ROM version 0 20 or above 79853 13004 S
10. 5 Screws M3 6 mm long Photo Sensor Grating Assembly Service Handbook for 1050 Series of HPLC Modules 11 2001 691 VWD Parts Information Filter Assembly SSS Filter Assembly Table 7 Filter Assembly Description Part number 1 Filter Assembly 79853 67903 2 Lever Position Sensor 3 Position Sensor Filter 79853 61107 4 Screws M3 6 mm long Figure 8 Filter Assembly S an S SA rose A SA SA SS S S S EEA fos A K BS ST Ps Sospan Tanana S ES OSS ESS se weet 692 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 8 NOTE VWD Parts Information Standard Flow Cell C SST Ti Standard Flow Cell C SST Ti Standard Flow Cell C SST Ti Item Description Part Nmber Oty 1 Cell Screw 79853 27201 2 Conical Spring 10 pk 79853 27203 10 3 Ring 2 pk 79853 27202 2 4 Gasket 1 PTFE 5 Window Quartz 6 Gasket 2 PTFE 7 Gasket 3 PTFE STD Flow Cell 8 mm SST see Note below STD Flow Cell 8 mm Ti 79853 60011 PEEK Inlet Tubing Kit 5062 8522 Cell Kit STD includes items 4 2x 5 2x 6 79853 68718 1x and 7 1x 5 Window Quartz Kit 79853 68719 2 4 Gasket 1 PTFE Kit STD 10 pk 79853 68720 10 6 Gasket 2 PTFE Kit STD 5 pk 79853 68721 5 7 Gasket 3 PTFE Kit STD 5 pk 79853 68722 5 The original STD flow cell 79853 60008 was replaced in June 1995 by the D version 79853
11. During Display Test every dot on the VFD module is set and you have to confirm it by yourself If display is black or shows missing dots 1 Check connection of flat ribbon cable DCB to KDI LL Replace KDI The Leak Sensor Test checks the leak sensor and the leak sensor circuit but not for a leak resulting from the cell The voltage applied is measured using built in 8 bit ADC A D Converter of the CPU as well as temperature compensation voltage from a second thermistor If the LS gt LL L SENSOR TROUBLE is displayed The range during turn on should be LS 0 63 4 06 V and LL 2 82 4 00 V 11 Use 33 LEAK S VOLT to verify the values see message LEAK DETECTED 1 Check connection of leak sensor to DCB LI Replace leak sensor m Replace DCB During Voltage Test this message is displayed if the voltages exceeds the tolerance If a voltage is not correct it is displayed for a short moment for example 24V TROUBLE 1 Check voltages using function 34 VOLTAGE TEST DC Voltages Voltages Used for 12 V 1 V filter and grating motor 15 V 15 V 1 V analog circuits 24 V 4 8V leak sensor fans m Replace DCB 1 Replace Power Supply Service Handbook for 1050 Series of HPLC Modules 11 2001 613 ADC TROUBLE EEROM DATA LOST EEROM DATA LOSTO EEROM DATA LOST1 EEROM DATA LOST2 EEROM DATA LOST3 NOTE VWD Diagnostic amp Troubleshooting Information Error Messages Bef
12. Figure 7 Signal Output Display Out Signal Analog Out Digital In Out Control of Deuterium Lamp VWD Electronic Information Detector Controller Board DCB D A Conversion VOLTAGE REFERENCE 10V 12 BIT LOW PASS DAC FILTER 6 MHz gt DA INT lt x PW DATA BUS __ 16 BIT COUNTER ADDRESS BUS __ cs DAC DATA BUS YF LATCHES cs DA RNG The signal output is classified into three types Display Out Analog Out Digital In Out The display module receives ASCII codes via Keyboard Interface Board via the Data Bus On the Display Module the ASCII code is converted to display code and gives output on fluorescent display with 5 x 7 dots and 16 characters The Signal Analog Out is available at a BNC connector at the rear of the instrument It is generated by 20 bit D A converter The conversion cycle of the D A converter is about 5 5 ms The output of the D A converter is filtered by a low pass filter The output can be switched to Full Scale Output Impedance 1V 1000 Ohm 0 1 V 100 Ohm The digital signal is delivered to the GPIB Interface option via the Data Bus and is converted into an GPIB signal The deuterium lamp is ignited using Anode Current Trigger Voltage and Heater Voltage supplied from the Power Supply Unit The ignition procedures are controlled by the CPU signal The heater voltage is switched fro
13. You can repeat the procedure again Service Handbook for 1050 Series of HPLC Modules 11 2001 627 VWD Diagnostic amp Troubleshooting Information Service Control Functions Wavelength Calibration Check 22 HOLMIUMCHECK This control function is used for the automatic check of the instruments WL calibrations using the some specific lines on the Holmium oxide filter which is moved into the light path Table 7 Control Function HOLMIUMCHECK Key Operation Display Description 22 HOLMIUMCHECK Select function ENTER CHECK CLEAR to leave function ENTER to start the calibration check 360 8nm automatic verification 418 5nm automatic verification 536 4nm automatic verification HOLMIUM CHECK OK if within specification CLEAR to leave function DOWN 360 8nm 360 7nm displays measured value DOWN 418 5nm 418 4nm displays measured value DOWN 536 4nm 536 4nm displays measured value CLEAR 22 HOLMIUMCHECK to leave function If this test was not successful perform 20 0th CALIB and 21 WL CALIBRATION 628 Service Handbook for 1050 Series of HPLC Modules 11 2001 31 SET WL PARAM Table 8 VWD Diagnostic amp Troubleshooting Information Service Control Functions SET WL Parameter This control function is used for the confirmation of monochrometer parameters or for changing them directly PARM WLO is the number of steps of stepper motor from the position sensor while PARM WL656 is the number of step
14. sample and reference the information from the grating and filter position sensor leak sensor remote control lines and the GPIB interface Optical Unit contains all optical parts Keyboard Display entry and display of parameter GPIB Interface communication with external controllers via GPIB 566 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Overview Figure 1 Block Diagram of VWD PRE AMP FLOW CELL D2 LA D BS STEPPER MOTOR U POSITION Sener POSITION GRATING SENSOR CD STEPPER a MOTOR y Y 1 Y GAI FI CONTROL CONTROL i t MULTI Lens g PT D EXING DATA PROCESSING on FANS FILTERING i ADC REMOTE _ LOG CONVERSION SCALING pm ANALOG OUT USER INTERFACE DCB KEYBOARD DISPLAY INTERFACE pipe I DISPLAY Service Handbook for 1050 Series of HPLC Modules 11 2001 567 Figure 2 SPHERICAL MIRROR M3 SPHERICAL MIRROR M4 VWD Hardware Information Optical System Overview Optical System Overview Figure 207 shows the optical diagram of the 79853C VWD The radiation from the deuterium lamp is focused on a spherical mirror M1 The light beam passes then a plane mirror M2 the cutoff filter the entrance slit a spherical mi
15. 24 V 4 8V leak sensor fans Service Handbook for 1050 Series of HPLC Modules 11 2001 635 VWD Diagnostic amp Troubleshooting Information Service Control Functions ADC Noise 35 ADC NOISE The output signal of the ADC noise corresponds to 196 AD counts at the Analog Output with 1 V full scale setting The ADC noise must be within 10 counts 50 mV over a time of 10 minutes Figure 234 Table 13 Control Function ADC Noise Key Operation Display Description 35 ADC NOISE Select function ENTER TESTING ADC ADC noise is outputted Start plotter in ATTN 7 ZERO 50 Chartspeed 1 on HP 339X CLEAR 35 ADC NOISE Leaves function Figure 3 ADC Noise aoe ADC Noise Plot CHREY20W poe Noise 29mV 54841 S387 gt 07 hii i Ha an PAA A A AA WiN Z siad Un i i W Wal Wilt S460 74 4967 4887 2 B 4 6 3 Lo 636 Service Handbook for 1050 Series of HPLC Modules 11 2001 36 GRATING P S Table 14 VWD Diagnostic amp Troubleshooting Information Service Control Functions Grating Photo Sensor This control function can be used to check the function of the photo sensor and the drive mechanism of the grating The movement of the grating can be done automatically or step for step It will provide the step number and the voltage of photo sensor which detects the backward limit when the drive mechanism moves into the photo sensor The photo sensor voltage exceeds 3 V when the pho
16. 5 Cover PSC N A 19 Sheet Fan N A 6 Leak Sensor Board see page 687 20 Fan Assembly HIGH 79853 68502 7 Leak Sensor Assembly see page 687 21 Switch Bearing 79853 61903 8 Sheet Front Panel see page 687 22 Switch Bracket N A 9 Front Panel Assembly see page 687 23 Switch Shaft 79853 61901 10 Display Interface KDI 79853 66502 24 Switch Top 79853 61902 11 Display Module VFD 79853 66503 25 Cable DCB KDI 79853 61609 12 Cable KDI VFD 79853 61602 26 Optical Unit see page 688 13 STD Flow Cell Assembly see page 693 13 Semi micro Flow Cell Assembly see page 695 Transport Screw Kit contains 3 79853 68700 screws with washer and spacer 13 Ultra High Pressure Flow Cell see page 697 PEEK Inlet Tubing Kit 5062 8522 Assembly 13 Prep Flow Cell Assembly TI see page 698 PEEK Waste Tubing Kit 5062 8535 14 Bracket DPS N A If installed in instruments with PSC 66509 then PSC must be updated to 79853 66512 Service Handbook for 1050 Series of HPLC Modules 11 2001 683 VWD Parts Information Overall Diagram Overall Diagram Part 1 Figure 1 Service Handbook for 1050 Series of HPLC Modules 11 2001 684 Figure 2 VWD Parts Information Overall Diagram Overall Diagram Part 2 Service Handbook for 1050 Series of HPLC Modules 11 2001 685 VWD Parts Information Front Panel Parts Front Panel Parts Leak Interface Table 2 Leak Interface Description Part number Leak Interface Kit
17. Adjust the angle of the beam splitter inserting a hexagon wrench through the top cover screw hole to get maximum photocurrent for the reference Turn right to move image down and left for up Replace the blank screw in the top cover Repeat the 20 0TH CALIB and 21 WL CALIBRATION Refer to Replacement of Deuterium Lamp on page 652 Step 2 and 3 Use 45 COMPENSATE to compensate non linearity of the grating motor 674 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Cleaning of Optical Unit Parts Cleaning of Optical Unit Parts There are only a few parts within the optical unit that can be cleaned Table 2 Cleanable Optical Parts NOT CLEANABLE grating and all mirrors Touching or cleaning will result in decrease of reflaction intensity CLEANABLE filters holmium cutoff and 0 order calibration beam splitter photo diodes You can wipe the surface with ethanol In case you cannot remove stains the part has to be replaced Service Handbook for 1050 Series of HPLC Modules 11 2001 675 VWD Maintenance Information Upgrade to GPIB Upgrade to GPIB 1 Note the values for WL 0 and 656 nm using 31 SET WL PARAM just in case the EEROM looses the values Turn off the instrument Remove top cover of the instrument Remove plate at the rear panel above the fan Install the GPIB board in that location using the screws that hold the plate OOC O oe
18. Connect the GPIB cable to J12 of the DCB board GPIB Address Setting The GPIB address setting is done with a switch 1 at the rear of the GPIB board The factory setting is 10 position A Figure 12 Location of GPIB board HPIB Firmware J18 676 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 3 VWD Maintenance Information Performance Verification Performance Verification Noise and Drift Specifications Noise 1 5x 10 AU Drift 5 0 x 104 AU h What you need m m m a pump that can deliver bidistilled water at a rate of up to 1 ml min against a back pressure of about 200 bar a column we recommend our 100 x 4 6 mm i d 5 pm Hypersil ODS column a recording device that can accept the output signal from your detector and that has attenuation set to about 35 cm mV Preparations COOCOO Q Prime the pump and ensure there are no air bubbles in the system Thoroughly degas about 300 ml of bidistilled water Set pump to deliver bidstilled water at a flow rate of 1 ml min Set the attenuation to about 35 cm mV Set run time on recorder to 6 0 min Turn ON line power and deuterium lamp Set wavelength to 254 nm response time to 1 second and output voltage to 1V Service Handbook for 1050 Series of HPLC Modules 11 2001 677 Figure 13 VWD Maintenance Information Performanc
19. HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information This chapter provides information on error messages and diagnostic features of the 1050 Variable Wavelength Detectors VWD Diagnostic amp Troubleshooting Information This section provides information on the e diagnostic routines e error messages e user contol functions e service control functions 610 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information Self Diagnosis Self Diagnosis At power on and after lamp ignition the instrument checks itself for correct operation In case of malfunctions error messages will inform the operator on the fault During Power On The following tests are done automatically during power on They are descibed on the next pages together with the error message e Vaccuum Fluorescent display e ROM and RAM e Display e Leak sensor e Voltages e A D Converter e EEROM Data e Grating Drive During Normal Operation The following tests are done automatically during normal operation e for light intensity e for filter movement e for leaks Service Handbook for 1050 Series of HPLC Modules 11 2001 611 ROM TEST FAILED RAM TEST FAILED VWD Diagnostic amp Troubleshooting Information Error Messages Before Lamp Ignition Error Messages Before Lamp Ignition Error messages may come up during the power on state or the normal operat
20. LOCAL BUS LEAK SENSOR gt z LEAK LEVEL gt ADDRESS POS SENSOR FIL gt SELF N DECODER POS SENSOR GR gt CHECK P S STATUS gt A D O LAMP STATUS gt _ STEPPER Q t 15V 12V 24 4 MOTOR D v BUZZER lt CONTROL a o v ROM a see 32KBYTE W VOLTAGEL_s 415V INPUT 19V REGU LATOR 15V o Ha 7 z RAM o Rg ud bay meee OUTPUT GNDA gt GNDD gt a PORT EEPROM 2KBYTE Lh ies ma YD Vv oe E lt Al Zz lt O oO A lt QO Service Handbook for 1050 Series of HPLC Modules 11 2001 593 Signal Processing VWD Electronic Information Detector Controller Board DCB Analog Sections The light beam from the SAMPLE and REFERENCE enters the photodiode respectively Its current output is converted into a voltage by pre amplifier The gain of the pre amplifier can be changed into four stages with CPU control The voltage signal from the pre amplifiers are multiplexed and converted into digital form by a 18 bit A D Converter The conversion time of A D Converter is 25 ms and is performed cyclically in the order of Zero Sample Reference Zero GNDA Electrically zero calibration is done while balancing Digital data is transmitted to the CPU digitally filtered based on response time and converted into the absorbance unit using the equation shown below AU log REF log SAMP The input voltage range
21. STD 79853 27203 10 pk 3 Ring same as STD 79853 27202 2 pk 4 Gasket 1 PTFE same as STD 79853 68720 10 pk 5 Window 1 Quartz same as STD 79853 68719 2 pk 6 Gasket 2 PTFE 0 1 for 0 9 ul 6 Gasket 2 PTFE 0 2 for 1 8 ul 6 Gasket 2 PTFE 0 5 for 4 4 ul 6 Gasket 2 PTFE 1 0 for 8 8 ul 7 Window 2 Quartz 8 Gasket 3 PTFE Preparative Flow Cell complete 79853 60012 PEEK Tubing Assembly 5062 8522 Cell Kit PREP includes items 4 1x 5 1x 79853 68725 6 1 of each size 7 1x and 8 1x Window Quartz Kit PREP includes items 5 1x 79853 68726 7 1x Gasket Kit PREP includes one of each sizeof 79853 68727 gasket 2 698 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information Preparative Flow Cell Ti Figure 12 Preparative Flow Cell Ti av SS ah Service Handbook for 1050 Series of HPLC Modules 11 2001 699 VWD Parts Information Cuvette Holder Cuvette Holder Table 13 Control Module Parts Item Description Part Number Cuvette Holder 79853 60016 For informationon the use of the cuvette holder refer to Using the Cuvette Holder on page 658 Figure 13 Cuvette Holder Q 700 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 14 VWD Parts Information Accessories Accessories These parts are shipped with the 79853C VWD Accessories Description Part number QTY Manual Get
22. clean certain parts of the flow cell you should have a clean working area Refer to Standard Flow Cell C SST Ti on page 693 and the following pages for detailed flow cell schematics Flow Cell Maintenance Kits There are several maintenance kits available with replacement parts for the flow cells For details see Standard Flow Cell C SST Ti on page 693 and the following pages Replacing Cell Parts For the detailed procedures refer to the Operating Manual LI Remove flow cell 1 Unscrew the cell screw using a 6 mm hexagon wrench see Figure 238 on page 656 item 1 LJ Remove inner parts carefully and place them in the correct order in front of you 1 Replace window gaskets or other parts as needed Once a gasket has been compressed or spoiled on the surface DO NOT use it again A used gasket may cause leakage 1 Insert all parts in the correct order LI Tighten the cell screw 1 Perform a Leak Test on page 657 Service Handbook for 1050 Series of HPLC Modules 11 2001 655 VWD Maintenance Information Flow Cell Maintenance Figure 2 Standard Flow Cell Flushing Procedure Cleaning the cell by using a glass syringe m m m m m Flush with iso Propanol Flush with bidistilled water Flush with nitric acid water 5 95 Flush with bidistilled water Flush with iso Propanol CAUTION This concentration of nitric acide is dangerous and proper
23. describe the replacements of parts separately It is important that only one assembly mirror grating beam splitter is changed and calibrated at a time Otherwise you will lose correct optical assembly alignment during the calibration process Do not remove the Entrance Slit Holder nor loosen it Otherwise the optical unit has to be exchanged completely The photocurrent readings with test slit installed are much lower than with the standard slit 712 Service Handbook for 1050 Series of HPLC Modules 11 2001 NOTE Figure 3 VWD Enhanced Optical Unit Information Installing the Test Slit Installing the Test Slit The small diameter of the test slit allows a straight forward alignment of the optical path It enables the lamp image to be positioned optimally This ensures correct illumination of the Entrance Slit and the Reference Slit This procedure has to be carried out at the beginning of all replacement procedures Turn on the detector and the lamp Set the wavelength to 250 nm Carefully remove the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 Remove the standard slit from the entrance slit holder using a pair of tweezers and place it safe Replacing the Entrance Slit entrance slit assembly entrance slit mirror 1 assembly test slit Service Han
24. from the instrument Remove main cover and disconnect all connectors from DCB Replace DCB after loosening the 3 fixing screws Fix all screws and reconnect all connectors to the DCB OO COCO O Oo Carefully remove the firmware ROM from removed DCB and insert it on new DCB L Replace main cover and turn the instrument ON L Turn lamp on and wait some minutes to stabilize the lamp L1 Perform functions 20 0th CALIB 21 WL CALIBRATION 44 DAC CALIB and 45 COMPENSATE to input the detector specific parameters into the EEROM DCB Firmware LI Disconnect the power from the instrument Remove main cover Carefully replace the firmware ROM Replace main cover and turn the instrument ON m m m m Turn lamp on and wait some minutes to stabilize the lamp Service Handbook for 1050 Series of HPLC Modules 11 2001 661 VWD Maintenance Information Replacing Display Boards Replacing Display Boards L Remove front panel assembly and disconnect cables between front panel and DCB LI Unscrew the six screws which hold the plastic part at the metal frame except the two screws that fix the leak assembly LI Disconnect the grounding cable and the cable between the two display boards Ll Replace the defective board 1 To loosen the flat cable 3 from the keyboard press a flat screwdriver onto the two noses on each side of the connector 1 to release the upper part 2 of the connector Figure 4 Rel
25. of the integrator is 0 5 9 5 V 594 Service Handbook for 1050 Series of HPLC Modules 11 2001 Figure 5 VWD Electronic Information Detector Controller Board DCB Block Diagram DCB A D Conversion A S INPUT 7s CHANNEL J P CANNEL SELECT 2 STAGE ELECT p AAAA GAIN LATCH DATA BUS VV VV y Z INPUT gt STAGE m Y VOLTAGE D A e oe REFERENCE SELF CHECK tov lt BNC VOLTAGE Z K SWITCH 1 2 kHz Y m INTEGRATOR AND COMPARATOR V 8 2V SWITCH K FLIP FLOP K 24 MHz REFERENCE VOLTAGE CONTROL we 10V we tu 4 BIT x 4 LSB Z COUNTER TO CPU 9 3X 16 BIT COUNTER V K 1 2 kHz 3 2 gt DATA BUS 16 MSB A D INT lt CONVERSION COUNTER 16 USB PERIOD INHIBIT COUNTER K ADDRESS BUS lt CS Service Handbook for 1050 Series of HPLC Modules 11 2001 595 A D Converter Figure 6 D A Converter VWD Electronic Information Detector Controller Board DCB Method for the A D conversion is feedback type Pulse Width Modulation PWM A D Converter INTEGRATOR COMPARATOR PWM U1 v OUTPUT p rT a COUNTER CPU fl
26. the original C optical unit in June 1995 The end of support EOS for the C optical unit parts will be August 1 2006 with all 1050 series HPLC modules Service Handbook for 1050 Series of HPLC Modules 11 2001 561 VWD General Information Repair Policy Repair Policy The 1050 VWD is designed that you can access all components easily You can recalibrate wavelength using control functions Customers are able to maintain certain parts of the 1050 VWD see Operator s Handbook For details on repair policy refer to Repair Policy on page 38 in chapter 1050 Common Information 562 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 1 VWD General Information Specifications Specifications Specifications of 79853C VWD Detection Type Noise Drift Wavelength Range Wavelength Accuracy Wavelength Reproducibility Bandwidth Linear Absorbance Range Response Time Spectra Light Source Flow Cells Display Control Double beam photometer lt 1 5x 10 AU peak to peak at 254 nm flowing water at 1 ml min 1 second response time 10 90 standard flow cell lt 5x107 AU hour at 254 nm after warm up 190 600 nm settable in 1 nm increments 2 nm 0 3 nm 6 5 nm Better than 1 up to 1 2 AU using acetone at 265 nm 0 25 1 or 4 seconds 10 90 user selectable Storage of 1 spectrum during run scan rate 10 nm sec range from 190 to 600 nm selecta
27. 045 Shows the photocurrent of reference and sample side with gain 1 DOWN G2 R0 227 S0 089 Shows the photocurrent of reference and sample side with gain 2 DOWN G4 R0 455 S0 179 Shows the photocurrent of reference and sample side with gain 4 DOWN G8 R0 914 S0 358 Shows the photocurrent of reference and sample side with gain 8 The displayed values should double each time If not change wavelength to 500 nm or change DCB CLEAR 42 PREAMP GAIN Leaves function 644 Service Handbook for 1050 Series of HPLC Modules 11 2001 43 EEROM TEST Table 22 VWD Diagnostic amp Troubleshooting Information Service Control Functions EEROM Test This control function checks the EEROM and is used as factory test Control Function EEROM Test Key Operation Display Description 43 EEROM TEST Select function ENTER EEROM TEST The blinking indicates the checked section EEROM TEST OK Test was successful If test failes replace DCB CLEAR 43 EEROM TEST Leaves function Service Handbook for 1050 Series of HPLC Modules 11 2001 645 44 DAC CALIB Table 23 VWD Diagnostic amp Troubleshooting Information Service Control Functions DAC Calibration This control function does a scaling between lower 12 bits and upper 8 bits of DAC and an automatic recalibration of the DAC internal voltages It should be performed when the noise is higher than expected and a DAC non linearity is found The test ru
28. 1050 Series of HPLC Modules Service Handbook Variable Wavelength Detector 79853C our lt S A A oes gt oe Agilent Technologies Copyright Agilent Technologies 2001 All rights reserved Reproduction adaption or translation without prior written permission is prohibited except as allowed under the copyright laws Part No NONE 11 2001 Printed in Germany Warranty The information contained in this document is subject to change without notice Agilent Technologies makes no warranty of any kind with regard to this material including but not limited to the implied warranties or merchantability and fitness for a particular purpose Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material IMPORTANT NOTE This version of the 1050 service manual includes all sections from the 01050 90102 edition 4 1995 The series I opticals information 79854A MWD and the 79853A VWD information has been removed products went out of support during 2000 Part numbers have been updated as of 11 2001 Contact your local Agilent support office in case of part number issues or upgrades The latest version of
29. 15V a Input JI Wel _ es oes 15V 19 a i B a A 15V 2 ae SAME f KIAK gle T H Voltage Switch J2 ov I K Selector Stepper 12V 19 awien Motor 5V J8 j j j 7 5 7 g g Position 5V 5V z Sensor 5 J7 Stepper 12V ov fe Motor 5V Power Supply Unit J6 717 J4 20 Posion 5V 027 Sensor J5 i Connection Board Leak de Sensor en het HPB B J3 5V lt lt X lt IF Board Fan Q lt e 24 p12 78 11 Fan Q lt e 24 10 Remote Display Key Disp Control odule IF Board Controller Board D Remote KIELE IE 5v Control bi7 16 E DTe Test 1 Connector Analog Out v Key Service Handbook for 1050 Series of HPLC Modules 11 2001 589 Table 2 NOTE Main Functions VWD Electronic Information Detector Controller Board DCB Detector Controller Board DCB Repair Level EPROM and Board Part Numbers for DCB tem Part Number Comment DCB Exchange 79853 69511 for use with PSC 66512 DCB 79853 66511 for use with PSC 66512 DCB 79853 66506 OBSOLETE for use with PSC 66509 OBSOLETE Firmware ROM DCB 79853 13005 If the DCB board is replaced by 79853 66511 version the PSC board MUST be changed to 79853 66512 The main functions of the Detector Controller Board DCB are CPU signal processing display analog output digital input output A D conversion D A conversion control of Optical Unit deuterium lamp ignition g
30. 2 Step 2 and 3 If grating has been moved use 45 COMPENSATE to compensate non linearity of the grating motor Set wavelength to 254nm Place cover on optical unit do not fix the screws at this time Excute control function 16 PHOTOCURRENT Remove the blank screw for mirror M4 adjustment Figure 243 on page 666 on the top cover leave the blank screw for beam splitter adjustment in Adjust the angle of the mirror M4 with inserting a hexagon wrench 1 5 mm through the top cover screw hole to get maximum photocurrent for the sample Turn right to move image down and left for up Replace the blank screw in the top cover Excute the 20 0th CALIB and21 WL CALIBRATION Refer to Replacement of Deuterium Lamp on page 652 Step 2 and 3 Install the beam splitter 8 Service Handbook for 1050 Series of HPLC Modules 11 2001 673 VWD Maintenance Information Optical Alignment Procedures Procedure 3 Reference Beam Alignment For items refer to Figure 247 on page 672 m m L Excute 40 0TH TEST ON with calibrated step value displayed Open the top cover and adjust the angle and direction of the beam splitter 8 so that the light beam illuminates the center of the reference slit 12 Place the cover on the optical unit Leave 40 0TH TEST ON and set wavelength to 254 nm Remove the blank screw for beam splitter adjustment Figure 243 on page 666 in the top cover Excute 16 PHOTOCURRENT
31. 4 Replace the spring 3 gasket 4 and complete motor assembly 5 Reinstall the grating 1 Refer to Optical Alignment Procedures on page 671 Grating Assembly Service Handbook for 1050 Series of HPLC Modules 11 2001 669 Figure 10 NOTE VWD Maintenance Information Replacements in the Optical Unit Replacing Filter Assembly Parts m m m m m Refer to Refer to Removing the Optical Unit on page 666 Remove the bottom plate of the optical unit Unscrew the 2 screws that fix the filter assembly Take out the complete assembly Replace the complete filter assembly sensor lever or the sensor Filter Assembly Replacing Mirrors Beamsplitter and Slits 1 Open the optical untit s top cover Ll Replace the item that need to be replaced 1 Refer to Optical Alignment Procedures on page 671 DO NOT touch the surface of the mirrors beam splitter and the grating 670 Service Handbook for 1050 Series of HPLC Modules 11 2001 WARNING VWD Maintenance Information Optical Alignment Procedures Optical Alignment Procedures Procedure 1 alignment after exchange of a specific part Procedure 2 alignment of complete optical sample path Procedure 3 alignment of complete optical reference path Be sure to wear a pair of safety or sun glasses Since the deuterium lamp emits intensive ultraviolet light it is dangerous to p
32. 53 68729 10 pk 5 Gasket 2 POLYIMIDE 79853 68730 5 pk High Pressure Flow Cell SST replaced by UHP Cell see Ultra High Pressure Flow Cell SST on page 697 PEEK Tubing Assembly 5062 8522 Cell Kit HP includes items 2 2x 3 2x 4 2x 79853 68728 and 5 1x NOTE The gaskets windows and rings are not compatible with the Ultra High Pressure Cell 79853 600013 that replaced the high pressure flow cell 79853 60009 For parts identification refer to Ultra High Pressure Flow Cell SST on page 697 696 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information Ultra High Pressure Flow Cell SST Ultra High Pressure Flow Cell SST Table 11 Ultra High Pressure Flow Cell SST Item Description Part Nmber Oty 1 Cell Screw 79853 27200 2 Ring PEEK UHP 3 Window Quartz Kit UHP 79853 68734 2 pk 4 Gasket 1 POLYIMIDE Kit UHP 79853 68737 2 pk 5 Gasket 2 POLYIMIDE Kit UHP 79853 68738 2 pk Ultra High Pressure Flow Cell SST 79853 60013 PEEK Tubing Assembly 5062 8522 Cell Kit UHP includes items 2 1x 3 2x 4 79853 68733 2x and 5 2x Figure 11 Ultra High Pressure Flow Cell SST Service Handbook for 1050 Series of HPLC Modules 11 2001 697 VWD Parts Information Preparative Flow Cell Ti Preparative Flow Cell Ti Table 12 Preparative Flow Cell Titanium Item Description Part Nmber Oty 1 Cell Screw same as STD 79853 27201 2 Conical Spring same as
33. 60000 For parts ID refer to Standard Flow Cell D Repair Parts on page 710 Service Handbook for 1050 Series of HPLC Modules 11 2001 693 VWD Parts Information Standard Flow Cell C SST Ti Standard Flow Cell C SST Ti Figure 9 a S NS Service Handbook for 1050 Series of HPLC Modules 11 2001 694 Table 9 Figure 10 VWD Parts Information Semi Micro Flow Cell SST Semi Micro Flow Cell SST Semi Micro Flow Cell SST Ti Item Description Part Nmber Oty 1 Cell Screw same as STD 2 Conical Spring same as STD 3 Ring same as STD 4 Gasket 1 PTFE same as STD 10 pk 5 Window Quartz same as STD 6 Gasket 2 PTFE Micro Semi Micro Flow Cell complete PEEK Capillary 400 mm Ig ID 0 12 mm Fitting for PEEK capillary 79853 27201 79853 27203 79853 27202 79853 68720 79853 68719 79853 68724 79853 60010 9021 1823 0100 1516 Cell Kit Micro includes items 4 2x 5 2x and 79853 68723 6 2x 10 p 2 pk 10 p 2 pk 10 p 2 pk Semi Micro Flow Cell SST Service Handbook for 1050 Series of HPLC Modules 11 2001 695 VWD Parts Information High Pressure Flow Cell SST High Pressure Flow Cell SST Table 10 High Pressure Flow Cell SST Item Description Part Nmber Oty 1 Cell Screw same as STD 79853 27201 2 Ring POLYIMIDE 3 Window Quartz 79853 68740 2 pk 4 Gasket 1 POLYIMIDE 798
34. 7 Gasket 3 D PTFE Qty 5 79853 68745 Figure 2 Standard Flow Cell D Repair Parts 710 Service Handbook for 1050 Series of HPLC Modules 11 2001 WARNING VWD Enhanced Optical Unit Information Repair and Mainenance Repair and Mainenance These procedures need special knowledge on servicing the 79853C VWD and should be done by trained Service Engineers only These procedures should be carried out in a room where the light can be reduced Since the deuterium lamp emits intensive ultraviolet light it is dangerous to perform optical alignment without eye protection Tools required 1 Test Slit supplied with mirror or grating assembly Pozi Driv PT1 hexagonal wrench 1 5 mm m m 1 hexagonal wrench 2 5 mm m pair of tweezers not too sharp points Pre requisites M Assure that the flow cell is clean flushed with water and bubble free L1 Remove detector from system J Place the detector on a bench LJ Remove the main cover Service Handbook for 1050 Series of HPLC Modules 11 2001 711 NOTE WARNING NOTE VWD Enhanced Optical Unit Information Additional Information Additional Information For additional information about replacements and the use of the Service control Functions refer to VWD Maintenance Information on page 649 and Service Control Functions on page 622 Replacements and Calibrations The following procedures
35. Assembly Replace and calibrate one mirror at a time Install the test slit see Installing the Test Slit on page 713 Carefully replace the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 3 Execute CTRL 20 0th CALIB 4 Activate service function CTRL 40 OTH TEST ON 5 Carefully remove the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 Unlock the reference aperture Unlocking the Reference Aperture on page 723 Center the reference slit on the white image by moving the aperture up or down see Figure 267 The image diameter is nearly equal to the reference slit diameter Algning the Reference Slit aise handle of reference aperture reference image 716 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Enhanced Optical Unit Information Replacing Mirror 3 or 4 Assembly 8 Install new mirror 3 or 4 assembly 10 11 12 13 14 15 16 Position the white image precisely onto the reference slit horizontally by rotating the mirror vertically using the setscrew of the mirror Carefully replace the cover of the optical unit Take care for the reference aperture handle De activate CTRL 40 0TH TEST OFF press CLEAR CLEAR and BALANCE Set A 250 nm Activate CTRL 16 PHOTOCURRENT Optimize the sample readings using the setscrew of mirr
36. CURRENT Optimize the sample readings using the setscrew of mirror 4 using the setscrew of mirror 4 through the hole in the optical unit cover see Figure 266 on page 715 Optimize the reference readings see Optimizing the Reference Readings on page 725 Install the standard slit and perform electronic calibrations see Installing the Standard Slit on page 726 720 Service Handbook for 1050 Series of HPLC Modules 11 2001 Figure 6 O location of lens assembly W 0 fy ee es cis 0 n casting of optical unit 9 VWD Enhanced Optical Unit Information Cleaning or Replacing the Lens Cleaning or Replacing the Lens The lens is located between lamp housing and casting and can be cleaned or replaced 1 Turn the detector off 2 Disconnect the lamp connector and all other connectors to the DCB board 3 Remove the optical unit completely from the instrument 4 Unscrew the four screws of the lamp housing and remove lamp housing Lens Assembly Location L Dzi lens assembly rS ULA spring washer lamp housing with lamp NOTE For easier repositioning the lens ring is marked with color paint see Figure 269 on page 722 The position of the marker could differ from instrument to instrument and may be different to the position shown in the figure 5 Remove clean or replace the lens If reusing old lens use markings for repositioning Service Handbook
37. D Enhanced Optical Unit Information This chapter provides information about the enhanced optical unit D 157 Compatibility 158 Support of Previous Optical Units 158 Introduction 159 Support Considerations 160 Service Handbook for 1050 Series of HPLC Modules 11 2001 7 Contents Prefix Change 160 Identification 160 Compatibility Matrix 160 Part Numbers for Enhanced D Optical Unit 161 Standard Flow Cell D Repair Parts 162 Repair and Mainenance 163 Tools required 163 Pre requisites 163 Additional Information 164 Replacements and Calibrations 164 Installing the Test Slit 165 Replacing Mirror 1 Assembly 167 Replacing Mirror 3 or 4 Assembly 168 Replacing the Grating or Grating Motor 170 Replacing the Beam Splitter 172 Cleaning or Replacing the Lens 173 Unlocking the Reference Aperture 175 Optimizing the Reference Readings 177 Installing the Standard Slit 178 8 VWD Additional Information This chapter provides additional information about the 1050 Variable Wavelength Detectors 179 Product History 181 Prefix Changes 181 DCB ROM Firmware Revisions 183 GPIB ROM Firmware Revisions 185 Hardware Changes and Service Notes 186 Modified Pre Amplifier Gain 186 8 Service Handbook for 1050 Series of HPLC Modules 11 2001 Contents Important Service Note 186 Service Handbook for 1050 Series of HPLC Modules 11 2001 Contents 10 Service Handbook for 1050 Series of HPLC Modules 11 2001
38. DJUSTMENT Removing the Optical Unit m m m m O Disconnect power from instrument Remove flow cell Remove top cover Disconnect all cables that go from the optical to the Detector Controller Board DCB Place the instrument on its left or right side Remove all nuts that fix the optical unit Take out the optical unit 666 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Replacements in the Optical Unit Replacing the PSC Board m m m m m L L Replacing Pre amplifiers or Photodiodes m m m m Refer to Removing the Optical Unit on page 666 Remove the PSC protection cover 2 screws Loosen the PSC board 3 screws Remove the lamp supply connector from the fan assembly Fed the wire carefully between fan cover and power supply cabinet If not possible loosen the fan cover Replace the PSC board and reuse the old cable to the DCB board When replacing the PSC protection cover take care for correct routing of the wire at the right side Remove the top cover and the front panel assembly Refer to Figure 244 for next steps Remove the cover that protects the pre amplifier To replace the pre amplifier boards LJ disconnect cable from pre amplifier board LJ unscrew the board LJ unsolder the wire from the photodiode LJ replace the board To replace the photodiode L remove cover plate of photodiode LJ un
39. O BALANCE 7 CLEAR 3 4 OUTP 1 RANGE om Gitte 0 Oo O JOL J ENTER JOS fH N N a NA NAN WwW Pe dh eco Sere A 602 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 5 Keyboard Display Interface Display Module VWD Electronic Information Keyboard Keyboard Electronics KDI VFD Repair Level Board Part Numbers for Keyboard Electronics Item Part Number Keyboard Display Interface KDI 79853 66502 Display Module Board VFD 79853 66503 Behind the frontpanel two electronic boards are located Keyboard Interface Board KDI and the Display Module Board VFD The Keyboard Interface Board KDI is connected to the DCB board and contains e interface between key matrix 6 x 4 and DATA bus e LED driver for the status messages ERROR NOT READY LAMP and RUN e buzzer The Display Module Board VFD is connected to the KDI and contains e CPU for control of latch driver e latch driver for the Vacuum Fluorescence Display FLD e DC DC converter to provide the voltages for the FLD Refer to Replacing Display Boards on page 662 when replacing this board Service Handbook for 1050 Series of HPLC Modules 11 2001 603 VWD Electronic Information
40. PLE and REFERENCE on D Diode Sample from C used for SAMPLE and REFERENCE on D Grating Assembly same part number as before but test slit added Firmware D rev 4 31 for D only added also to 79853 69014 15 and 79853 69511 DCB Slit Kit D Test slit plus STD slit with C version optical units with C version optical units part number 79853 68109 should only be used for 79853C optical until stock has expired NOTE The part numbers 79853 68110 68111 68112 68113 and 64605 include beside the test slit in addition a seal to close the hole for Mirror 4 adjustment setscrew Close the hole with this seal during replacements see Figure 266 on page 715 for the location Service Handbook for 1050 Series of HPLC Modules 11 2001 709 VWD Enhanced Optical Unit Information Standard Flow Cell D Repair Parts Standard Flow Cell D Repair Parts Table 2 Standard Flow Cell D Repair Parts Item Description Part Number STD Flow Cell D complete assembly Cell Screw Kits Cell Kit STD D consists of two windows two gaskets 1 one gasket 2 and one gasket 3 79853 60000 79853 27200 79853 68741 2 Conical Spring D Qty 10 79853 29100 3 Ring SST D Oty 2 79853 22500 5 Window Quartz D Oty 2 79853 68742 4 Gasket 1 D PTFE Qty 10 79853 68743 6 Gasket 2 D Aperture gold Qty 5 79853 68744
41. SCREWS AND SPACERS FOR TRANSPOTATION APPROX M4 12mm LONG PLUS SHAPED SCREW YOU CAN KEEP THE THREE SCREWS AND SPACERS ON THE REAR PANEL OF THE UNIT AS SHOWN IN THE FIGURE BELOW Cr p eens pema O o lt a MN E s anu Service Handbook for 1050 Series of HPLC Modules 11 2001 651 VWD Maintenance Information Replacement of Deuterium Lamp Replacement of Deuterium Lamp If the Deuterium lamp no longer ignites or the lamp emission has become too low and noisy the lamp has to be replaced The deuterium lamp is exchangeable by the user No adjustment is necessary For accurate wavelength setting excute user control function 20 0th CALIB and 21 WLCalibration after exchanging the lamp Step 1 Replacement 1 Turn the lamp off _J Loosen the two screws at the rear of the instrument which fix the cover and remove them Remove top cover Unplug the lamp and remove it Exchange the lamp no adjustment is required Reconnect the lamp Replace top cover OOOO O O Turn the lamp on 652 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Replacement of Deuterium Lamp Step 2 Oth Order Calibration The instrument scans for the maximum of the zero order light During Oth order calibration a filter for reduc
42. TE WARNING VWD Maintenance Information Replacements in the Optical Unit Replacements in the Optical Unit The repair level of optical unit parts is component level Refer to section Optical Unit C on page 688 for additional parts The information in this chapter is based on the original optical unit version C In June 1995 this optical was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 Be sure to wear a pair of safety or sun glasses Since the deuterium lamp emits intensive ultraviolet light it is dangerous to perform optical alignment without eye protection Be careful not to expose your eyes directly to the light coming from the lamp Service Handbook for 1050 Series of HPLC Modules 11 2001 665 VWD Maintenance Information Replacements in the Optical Unit Figure 7 Optical Unit BLANK SCREW FOR M4 MIRROR ADJUSTMENT 3 G9 CAUTION WARNING S REPAIR PROCEDURES REQUIRING ACCESS nowt REMOVE THIS COVER TO THE OPTICAL COMPARTMENT UNDER THIS COVER MUST BE DONE ONLY BY AL eta ee Gi wank niet ouR AUTHORIZED SEAVICE PERSONAL amp BLANK SCREW FOR BEAM SPLITTER A
43. Then the filter is always ON e The reference light beam is focussed far from the reference diode Flow cell should be clean and bubble free Check connection of filter motor Oc Check correct operation of filter with 39 FILTER TEST and photo sensor of filter with 37 FILTER P S L Check beam splitter alignment 616 Service Handbook for 1050 Series of HPLC Modules 11 2001 LEAK DETECTED Table 2 DATA UNDERFLOW DATA OVERFLOW VWD Diagnostic amp Troubleshooting Information Error Messages During Normal Operation Error Messages During Normal Operation LJ Enter service mode function 33 LEAK S VOLT and check leak sensor voltages Error condition is when LS gt LL The normal range should be Working Ranges for LS and LL LS signal LL signal 2 35 2 85 V 2 95 3 45 V 1 Ifthere is no leakage check the connection leak sensor to DCB LI Replace Leak Detector board 1 Replace Leak Sensor This message may come up only during BALANCING when the sample or referrence voltage is lower than 1 mV 1 Check whether the flow cell is in correct position and the screws are tightened 1 Check the connection of pre amplifier sample to DCB 1 Clean cell windows LI Replace photo diode assembly m Replace DCB This message may come up only during BALANCING when the sample or referrence voltage exceeds 9 4 V Check whether the flow cell is in correct position and the screw
44. ak handling leak output signal for shutdown pumping system Low voltages in major maintenance areas Environment Temperature range 5 to 55 C Humidity lt 95 non condensing Power Requirements Line voltage 100 120 or 220 240 VAC 10 Line frequency 48 66 Hz Power consumption 150 VA max Dimensions Height 133 mm 5 2 inch Width 325 mm 12 8 inch Depth 545 mm 21 5 inch Weight 14 kg 31 Ib 564 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information This chapter provides hardware information about the 1050 Variable Wavelength Detectors NOTE Table 1 VWD Hardware Information Overview The information in this chapter is based on the original optical unit version C In June 1995 this optical was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 Figure 206 on page 567 shows the block diagrams of the 1050 VWD 79853C The main components are Main Components Overview Component Purpose Power Supply DPS A provides all voltages within the instruments Detector Controller Board controls power supply grating stepper motor DCB cutoff filter keyboard display and GPIB interface It processes the signals coming from the pre amplifier boards
45. alibration Key Operation Display Description 21 WL CALIBRATION Select function ENTER 656nm xxxx step Displays the wavelength for calibration and steps based on present PARAM WL 656 ENTER or To continue calibration or CLEAR to abort Wait appr 30 seconds Xxxxstep y yyyV Starts the calibration scan from 656 nm 50 steps and seeks for maximum light intensity No key entry is allowed here Possible error message at this point may be CALIB FAILURE Indicates that there was no maximum found see CAL B FAILURE on page 619 DATA UNDERFLOW Indicates that not enough light reaches the sample photodiode lt 30 mV The next line will be shown if no error message occured 626 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 6 VWD Diagnostic amp Troubleshooting Information Service Control Functions Control Function WL Calibration Description Key Operation Display Xxxxstep y yyyV ENTER 656nm xxxx step after 2 seconds 656nm xxxxstep ENTER CLEAR ENTER 656 yyyystepOK after 2 seconds 21 WL CALIBRATION Displays the step that gave the maximum light intensity Displays the wavelength and step that gave the maximum light intensity Displays the new parameter at 546 nm and prompts the confirmation Takes the displayed step number Rejects the displayed step New parameter is written in EEROM and monochrometer is reset with new parameter End of calibration
46. alog output signal New DCB 79853 66511 and PSC 79853 66512 Boards have been introduced This note describes the optimization for drift problems Introduction of Enhanced Optical Unit see VWD Enhanced Optical Unit Information on page 705 Cuvette Holder for Wavelength Verification with Certified Standard Solutions available see Cuvette Holder on page 700 Check of photometric accuracy information on path lengths of flow cells see Correction factors for 79853C flow cells on page 575 734 Service Handbook for 1050 Series of HPLC Modules 11 2001 oe Agilent Technologies In This Book This manual contains technical information about the Agilent 1050 liquid chromatographs This manual is available as electronic version Adobe Acrobat Reader file only
47. arameters set by key input wavelength range response time mode The main clock 24 MHz is divided and distributed to 24 MHz A D Converter Count Pulse 12 MHz CPU Clock 6 MHz D A Converter Count Pulse 3 MHz GPIB Controller Key Display Controller Clock 1 2 KHz A D Converter Integration period After the power turns on for about 100 ms the RESET signal is supplied to CPU GPIB Controller and Key Display controller Also anytime when power voltage becomes less than 4 7 V the RESET signal is generated The CPU controls directly the Buzzer and the Stepper Motor Driver to move the filters on the filter assembly The stepper motor for the grating the input and the output port are connected to the internal BUS DATA ADDRESS and CONTROL and are controlled by the CPU The grating motor and the filter motor are driven by a motor controller IC and phase pattern are coming from CPU 592 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Electronic Information Detector Controller Board DCB Figure 4 Block Diagram DCB Digital TO ee A D KEYBOARDP A _ 6MHz CLOCK ND HP IB MHz GENERATION 1 2kHz 1 2MHz a LAMP CONTROL lt O DC MOTOR gt 5 CHANNEL SELECT C DRIVER FILTER Fi MUX SIGNAL TI z
48. attention to safety should be given Also the nitric acid flushing procedure is not a certain cure for a dirty cell It is to be used as a last attempt to salvage the cell before certain cell parts have to be replaced 656 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Flow Cell Maintenance Leak Test Remove the flow cell and connect the outlet tubing of the flow cell directly to the pump Then flow an appropriate solvent for example Isopropanol at 10 ml min After several minutes check the cell visually If it is necessary to check it for higher pressure put an appropriate stainless steel tubing which can build up a back pressure CAUTION DO NOT apply higher back pressure to the flow cell than maximum pressure of the flow cell Table 1 Pressure Rating on Flow Cells Type Pressure Standard Flow Cell 40 bar Ultra High Pressure Cell 400 bar Preparative Flow Cell 40 bar Semi Micro Flow Cell 40 bar Service Handbook for 1050 Series of HPLC Modules 11 2001 657 VWD Maintenance Information Using the Cuvette Holder Using the Cuvette Holder When required Tools required Parts required If your own standard should be used to checkout the instrument None Cuvette Holder 79853 60016 Cuvette with the standard e g NIST certified holmium oxide sample Figure 3 This cuvette holder can be placed instead of a flow cell in the variable wavelengt
49. avelengths 630 Service Handbook for 1050 Series of HPLC Modules 11 2001 32 FIX SIGNAL Table 9 NOTE VWD Diagnostic amp Troubleshooting Information Service Control Functions Fix Signal If the 1050 VWD shows noise or drift problems this function can help to isolate the cause The reference or the sample diode can be set to an fixed electrical value to remove influences from the light path This function can also be used to retrieve the instrument profile of the instrument Control Function FIX SIGNAL Key Operation Display Description 32 FIX SIGNAL Select function ENTER REF CH FIXED The photocurrent of reference photo diode is a fixed electrical value DOWN or UP Select the parameter NORMAL NOT FIXED Return to normal condition neither photocurrrent is fixed SMP CH FIXED The photocurrent of sample photo diode is a fixed value ENTER 32 FIX SIGNAL Control function is displayed again If either channel is fixed the ERROR status lamp is blinking even after returning to analysis mode from service control mode This is an alarm to show that the instrument is abnormal condition You can return to normal condition by executing NORMAL NOT FIXED or switching off the power Service Handbook for 1050 Series of HPLC Modules 11 2001 631 Use of FIX SIGNAL VWD Diagnostic amp Troubleshooting Information Service Control Functions Use this function for L checking the noise
50. ble Post run plotting speed from 1 to 50 nm sec selectable plotting of background corrected spectrum Deuterium lamp from 1090 40 50 DAD Standard 14 ul volume 8 mm pathlength cell with 40 bar 588 psi pressure maximum Optional micro preparative high pressure cells are available Single line 16 character fluorescent display with real time display of operating parameters and or absorbance Integrated keyboard with function keys parameter editing during run possible keyboard lock optional control by computer or 3396 Series II integrator Service Handbook for 1050 Series of HPLC Modules 11 2001 563 Table 1 VWD General Information Specifications Specifications of 79853C VWD Parameters Wavelength output range response time zero offset balance spectrum acquisition Diagnostic Aids Wavelength calibration check with Holmium oxide filter Time programs Time programmable wavelength and output range storage of up to 5 time programs Analog Output One output user configurable as recorder or integrator For recorder 100 mV or 1 V output range from 0 001 to 4 AU user selectable for integrator 100 mV 2 AU full scale or 1 V 2 AU full scale user selectable Communications START input output STOP input output READY output SHUTDOWN output for synchronization with other LC modules Safety Aids Extensive diagnostics error detection and display via front panel LED s Leak detection safe le
51. ce Control Functions Figure 2 Example of Intensity Profiles DEFAULT D ADC CHANNEL A Fixed Reference 190 200 DEFAULT D ADC CHANNEL A Fixed Sample Service Handbook for 1050 Series of HPLC Modules 11 2001 633 33 LEAK S VOLT Table 10 VWD Diagnostic amp Troubleshooting Information Service Control Functions Leak Sensor Voltage You can check the leak sensor with this contol function in case of trouble Control Function Leak Sensor Voltage Key Operation Display Description 33 LEAK S VOLT Select function ENTER LS 2 70 LL 3 30 Displays LS Leak Sensor Voltage and LL Temperature compensation voltage Error condition is when LS gt LL The normal ranges are LS signal 2 35 2 85 V LL signal 2 95 3 45 V CLEAR 33 LEAK S VOLT Leaves function 634 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 11 Table 12 VWD Diagnostic amp Troubleshooting Information Service Control Functions Voltage Test 34 VOLTAGE TEST You can check some but not all voltages with this contol function Control Function Voltage Test Key Operation Display Description 34 VOLTAGE TEST Select function ENTER 15 0 22 9 Displays first set of voltages DOWN or UP 12 1 15 0 Displays second set of voltages CLEAR 34 VOLTAGE TEST Leaves function DC Voltages Voltages Used for 12 V 1 V filter and grating motor 15 V 15 V 1 V analog circuits
52. contains 79853 68731 Top Bottom and Tubing Figure 3 Leak Interface Top w 3 S 2 2 Zz Zz Tubing KZ rw ZZ z Bottom e 686 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information Front Panel Parts Font Panel Table 3 Front Panel Description Part number Front Panel Complete 79853 60203 Front Panel 79853 60202 1 Leak Sensor Board 79853 66510 2 Leak Sensor Assembly 79853 66111 3 Display Module Board VFD 79853 66503 A Cable KDI VFD 79853 61602 5 Display Interface KDI 79853 66502 6 Cable DCB KDI 79853 61609 O ring Leak Assembly 79853 82501 Figure 4 Front Panel 4 3 CN J17 Tire 1 J20 O 16 pi _ 6 2 1 Service Handbook for 1050 Series of HPLC Modules 11 2001 687 VWD Parts Information Optical Unit C Optical Unit C The parts identification of the optical unit is splitted into four sections e Optical Unit Inner Parts Top e Optical Unit Inner Parts Bottom e Grating Assembly e Filter Assembly e Flow Cell Assemblies NOTE The information in this chapter is based on the original optical unit version C In Jun
53. dbook for 1050 Series of HPLC Modules 11 2001 713 VWD Enhanced Optical Unit Information Installing the Test Slit 5 Carefully insert the test slit with round hole into the entrance slit holder The slit must sit flat on the holder with the white side towards the incoming light 714 Service Handbook for 1050 Series of HPLC Modules 11 2001 N NOTE Figure 4 aperture handle of reference O OH VWD Enhanced Optical Unit Information Replacing Mirror 1 Assembly Replacing Mirror 1 Assembly Install the Test Slit see Installing the Test Slit on page 713 Remove mirror 1 assembly Install new Mirror 1 assembly DO NOT remove or change its position of adapter plate underneath the mirror 1 assembly Position the lamp image onto the test slit hole horizontally by rotating the mirror vertically using the setscrew on the mirror Fix the mirror Install the Standard Slit and perform grating calibrations see Installing the Standard Slit on page 726 Carefully replace the cover of the optical unit Take care for the reference aperture handle Reassemble the detector Handle of the Reference Aperture hole seal of Mirror 4 for alignment cover of optical unit Service Handbook for 1050 Series of HPLC Modules 11 2001 715 NOTE Figure 5 VWD Enhanced Optical Unit Information Replacing Mirror 3 or 4 Assembly Replacing Mirror 3 or 4
54. e see Unlocking the Reference Aperture on page 723 Optimize the reference readings with the reference slit see Optimizing the Reference Readings on page 725 Install the standard slit and perform electronic calibrations see Installing the Standard Slit on page 726 Service Handbook for 1050 Series of HPLC Modules 11 2001 719 VWD Enhanced Optical Unit Information Replacing the Beam Splitter Replacing the Beam Splitter Install the test slit see Installing the Test Slit on page 713 Execute CTRL 20 Oth CALIB Carefully remove the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 Activate service function CTRL 40 OTH TEST ON 5 Unlock the reference aperture see Unlocking the Reference Aperture on page 723 Center the reference slit on the white image by moving the aperture up or down The image diameter is nearly equal to the reference slit diameter 7 Install the new beam splitter assembly 10 11 12 13 14 15 16 17 Position horizontally the white image center of the beam splitter onto the reference slit Fix the beam splitter after precise image fit Correct new vertical position with reference slit Carefully replace the cover of the optical unit Take care for the reference aperture handle De activate CTRL 40 OTH TEST OFF press CLEAR CLEAR and BALANCE Set A 250 nm Activate CTRL 16 PHOTO
55. e 1995 this optical was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 For complete optical unit C replacements use part number 79853 60015 This upgrades to D version see Part Numbers for Enhanced D Optical Unit on page 709 688 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information Optical Unit C Optical Unit C Inner Parts Top Table 4 Optical Unit C Inner Parts TOP Item Description Part Nmber Item Description Part Nmber Optical Unit complete assembly 79853 69015 7 Grating Assembly see page 691 Plate Optical Top 79853 04108 8 Beam Splitter 79853 20402 1 Deuterium Lamp Assembly 79883 60002 9 Pre Amplifier Board SAMPLE 79853 66507 Lamp housing 79853 22006 10 Sensor Sample 79853 61109 2 Filter Assembly see page 692 11 Pre Amplifier Board REF 79853 66508 3 Entrance Slit Assembly 79853 23103 12 Sensor Reference 79853 61110 4 Mirror 1 79853 68107 Reference Slit 79853 23104 5 Mirror 2 79853 68108 Spacer Reference Sensor 79853 24702 6 Mirror 3 or 4 79853 68109 PTFE Ring 53C 79853 24500 Figure 5 Optical Unit C Inner Parts TOP
56. e Verification Starting a run L Give the optical unit time to warm up and stabilize J Start a run The baseline noise should not exceed 7 5 pV 1 5 x 10 AUI equivalent to 4 5 mm at attenuation 3 on a 3390 2 3 integrator and equivalent to 9 mm at attenuation 3 on a 3394 6 integrator Example of Noise Plot mal NVv 0102 0 ADC CHANNEL A 146 4 146 3 eT 146 2 146 1 4 146 Time gt 5 0 10 0 15 0 20 0 25 0 30 0 35 0 40 0 45 0 50 0 55 0 This plot was taken with e a 79853C VWD e an 35900C A D converter e a LC DOS workstation e flow cell windows removed but gasket 2 installed It shows that the drift on this example is lt 1 x 104 AU hr and the noise at about lt 1 x 10 AU 678 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 4 VWD Maintenance Information Performance Verification Scaling Factors The table below shows the scaling factors for the 339XA family of integrators The 3390A 3392A and 3393A have a full scale deflection of 75 mm The 3394A and the 3396A have a full scale deflection of 150 mm they also have an attenuation range between 8 and 36 Scaling Factors on 339X integrators ATTN mV full scale mAU full scale 3 0 125 2 0 25 1 0 5 0 1 co A N o ama N oo A O N o N 1024 0 25 0 5 1 co A N 1024 2048 Service Handbook for 1050 Series of HPLC Modules 11 2001 679 VWD Maintenance Inf
57. each DAC bit used for DAC calibration These parameters can be re written at the factory only J Perform 41 DAC TEST If OK then leave it as it is If NOT OK then continue 614 Service Handbook for 1050 Series of HPLC Modules 11 2001 WL SET TROUBLE VWD Diagnostic amp Troubleshooting Information Error Messages Before Lamp Ignition L Perform 44 DAC CALIB The default values of the offset parameters are taken now LJ Perform 41 DAC TEST If OK then leave it as it is If NOT OK then replace DCB board During the initialization of the grating motor position the motor moves backward to the home position where position sensor detects the limit If it is not able to detect the limit WL SET TROUBLE is displayed 11 Check connection of position sensor and grating drive motor 11 Switch OFF lamp remove top cover of optical unit to observe movement of grating mirror changing wavelength If grating will not rotate after changing the wavelength replace DCB or the Grating Driver Assy LJ Using 36 GRATING P S you can move the grating shaft automatically or stepwise by pressing the down up key Normally in the position of about 200 steps the output voltage of the position sensor will change from LOW to HIGH detecting the limit position If the output voltage will never change the position sensor is defective or the grating drive assembly has a problem and has to be replaced Service Handbook for 1050 Seri
58. ease of connector 662 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Replacing the Leak Interface Replacing the Leak Interface Leak Sensor Assembly Ll Remove the flow cell Ll Remove top cover J Disconnect leak cable from DCB and the flat ribbon cable from DCB to keyboard at the KDI board LI Unscrew the screws that fix the front panel 1 Remove front panel LI Replace sensor board or complete assembly Figure 5 Leak Sensor Assembly l J Z TE STA cre hs N Thermistor Lever Leak out Leak sensor assy Service Handbook for 1050 Series of HPLC Modules 11 2001 663 VWD Maintenance Information Replacing the Leak Interface Leak Interface 1 Carefully remove the leak interface 1 Replace the defective item All three parts top bottom and tubing are coming together as kit I Install the leak interface Figure 6 Leak Interface 664 Service Handbook for 1050 Series of HPLC Modules 11 2001 NO
59. ed The fans are connected to 24 VDC on the DCB Board Service Handbook for 1050 Series of HPLC Modules 11 2001 571 NOTE NOTE Figure 5 VWD Hardware Information Optical Unit Optical Unit The information in this chapter is based on the original optical unit version C In June 1995 this optical was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 The Optical Unit houses all parts from the deuterium lamp to the photodiode pre amplifiers The repair level is component The optical unit is also available as assembly Optical Unit 572 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Optical Unit Flow Cells There are several flow cells available as stainless steel or as titanium version see Table 138 on page 574 The flow cell Figure 211 can be exchanged easily No adjustments are necessary After disassembling of the flow cell gaskets and windows can be replaced refer to section Flow Cell Maintenance on page 655 There are several kits set up with replacement parts like gaskets and windows see Standard Flow Cell C SST Ti on page 693 and the following pages Figure 6 STD Flow Cell NOTE A
60. ed UP TESTING BIT 5 Bit 5 is tested UP TESTING BIT 6 Bit 6 is tested UP TESTING BIT 7 Bit 7 is tested CLEAR 41 DAC TEST Leaves function DAC DC Values Bit mV Decription 0 27 1 32 jump of 5 mV 2 42 jump of 10 mV 3 62 jump of 20 mV 4 104 jump of 40 mV 5 22 GND plus offset voltage 6 185 jump of 80 mV 7 511 about half of maximum output voltage If you do not see a linear response replace the DCB board 642 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information Service Control Functions An AC voltage is added to the DC output and should be lt 0 8 x 10 AU Figure 4 DAC Test Bit 0 AC mAU DAC TEST BIT 0 115 294 115 292 115 288 115 286 Figure 5 DAC Test Bit 7 AC AU DAC TEST BIT 7 1086 89 1086 88 1086 87 1086 86 1086 85 1086 84 1086 83 1086 82 If one or more tested bits are out of limit then perform 44 DAC CALIB Service Handbook for 1050 Series of HPLC Modules 11 2001 643 42 PREAMP GAIN Table 21 VWD Diagnostic amp Troubleshooting Information Service Control Functions Pre amplifier Gain This control function allows the check of gain switching on the DCB The gain can be switched to 1 2 4 and 8 Control Function Pre Amplifier Gain Key Operation Display Description Change wavelength to 500 nm to reduce intensity of light 42 PREAMP GAIN Select function ENTER G1 R0 115 S0
61. erform optical alignment without eye protection Be careful not to expose your eyes directly to the light coming from the lamp Procedure 1 Simple Alignment When replacing just a single part in the light path other than grating parts M4 and beam splitter this procedure can be performed LJ Execute 40 0th TEST which shows the step number from 31 SET WL PARM and watch the location of the images on the slit assemblies entrance and reference slit and the cell center Exchange the part Refer to Replacing for more information Position the part so that the image centers on entrance and reference slit Fix the part O O O O If image on exit slit is too high or low follow procedure Procedure 2 Sample Beam Alignment on page 672 L If image on reference slit is too high or low follow procedure Procedure 3 Reference Beam Alignment on page 674 4 Excute the 20 0th CALIB and21 WL CALIBRATION 11 Refer to Replacement of Deuterium Lamp on page 652 Step 2 and 3 Service Handbook for 1050 Series of HPLC Modules 11 2001 671 M1 M2 Entrance Slit M3 Figure 11 VWD Maintenance Information Optical Alignment Procedures Procedure 2 Sample Beam Alignment For items refer to Figure 247 m m m m L Remove the entrance slit 3 Turn the deuterium lamp 1 on Make sure the light spot covers over mirror M1 4 Adjust the direction of mirror M1 4 so that the light beam c
62. ervice Handbook for 1050 Series of HPLC Modules 11 2001 731 VWD Additional Information DCB ROM Firmware Revisions Table 2 DCB ROM Firmware Revisions Revision Major Changes Comments 4 24 Removed bug EEROM DATALOST1 Released for 79853C in June 1992 Removed bug Wavelength accuracy Started with serial number between 360 nm and 486 nm 3225J00773 For replacements use part number 79853 13005 4 24 NEEDS GPIB ROM version 0 20 or above 79853 13004 4 31 For Enhanced Optical Unit D only Released for 79853C in June 1995 Started with serial number 3522J04305 For replacements use part number 79853 13000 4 31 NEEDS GPIB ROM version 0 20 or above 79853 13004 732 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 3 VWD Additional Information GPIB ROM Firmware Revisions GPIB ROM Firmware Revisions The table below lists all GPIB ROM firmware revisions for the 79853A C GPIB ROM Revisions Revision Major Changes Comments 0 04 0 05 0 08 0 09 0 20 LC APPACK problem BUFFER OVERFLOW message preventing run buffer overflow Also useable for 79853C PHOENIX problem prevents hang ups at power on if VWD is switched on after PHOENIX has been switched on PHOENIX problem prevents VECTRA 486 hang ups additional changes for future PHOENIX enhancements First official realease for 79853A Released for 79853A Released for 79853A and 79853C Fo
63. es function 640 Service Handbook for 1050 Series of HPLC Modules 11 2001 40 0TH TEST OFF Table 18 VWD Diagnostic amp Troubleshooting Information Service Control Functions Zero Order Test This control function is used to move the stepper motor into the zero order position and has to be performed during the alignment of the grating Control Function Zero Order Test Key Operation Display Description 40 0TH TEST OFF Select function The test is still off and the grating is on set parameter wavelength ENTER Oth STEP NOnnn Take the number of steps from 31 SET WL PARM default or use 200 steps ENTER OTH TEST ON The stepper motor is moved to zero order position CLEAR 40 0TH TEST OFF Leaves function and grating is turned to set wavelength Service Handbook for 1050 Series of HPLC Modules 11 2001 641 41 DAC TEST Table 19 Table 20 VWD Diagnostic amp Troubleshooting Information Service Control Functions DAC Test This control function is used to check the D A converter by setting different bits An AC voltage is added to the DC output and should be lt 0 8 x 10 AU Control Function DAC Test Key Operation Display Description 41 DAC TEST Select function ENTER TESTING BIT 0 Bit 0 is tested UP TESTING BIT 1 Bit 1 is tested UP TESTING BIT 2 Bit 2 is tested UP TESTING BIT 3 Bit 3 is tested UP TESTING BIT 4 Bit 4 is test
64. es of HPLC Modules 11 2001 615 LAMP ERROR LOW ILLUMINATION FILTER ERROR VWD Diagnostic amp Troubleshooting Information Error Messages After Lamp Ignition Error Messages After Lamp Ignition 1 Check connections of lamp connector PSC board and DPS to DCB m Replace lamp DPS DCB Light intensity of deuterium lamp is checked after lamp ignition at the wavelength of 250 nm If the reference voltage at 250 nm is lt 0 6 V the detector will check the reference voltage at 500 nm and if lt 0 12 V LOW ILLUMINATION will be displayed In this case the detector will never return to the original wavelength It will remain at 500 nm This test is skipped if WL SET TROUBLE is displayed and unable to set the wavelength 1 Check the lamp image on the entrance slit If the image does not cover the slit properly adjust mirror M1 1 Check connection to pre amplifiers LI Replace the lamp for deterioration of lamp 1 Replace the mirrors M1 and M2 for deterioration of mirrors During Filter Test the second order light cutoff filter is tested by inserting it at the wavelength of gt 370 nm and measuring the change of light intensity If an error is found FILTER ERROR is displayed This message comes up if e The reference current at 220 nm is more than 2 00 and at 500 nm more than 1 16 of the value at 220 nm Then the filter is always off e The photocurrent at 220 nm is less than 2 00 and at 500 nm more than 0 04
65. for 1050 Series of HPLC Modules 11 2001 721 VWD Enhanced Optical Unit Information Cleaning or Replacing the Lens Figure 7 Lens Position Q marker lens assembly NOTE The more plane lens side with smaller aperture faces towards the lamp NOTE If a new lens is installed mirror 1 assembly has to be realigned after this procedure see Replacing Mirror 1 Assembly on page 715 6 Reassemble the flat spring 7 Replace the Lamp housing and tighten it 8 Reassemble the detector 722 Service Handbook for 1050 Series of HPLC Modules 11 2001 NOTE NOTE Figure 8 VWD Enhanced Optical Unit Information Unlocking the Reference Aperture Unlocking the Reference Aperture Only necessary if required during a replacement procedure For performance reasons the reference aperture is fixed by one screw only and has to be unlocked prior to any replacement calibration within the optical unit 1 Unscrew the front panel and place it in front of the detector to have access to the reference pre amplifier area When moving the front panel assure that the keyboard cable is not partially disconnected damage to the electronics is possible 2 Unscrew the right screw of the reference pre amplifier cover and loosen the left screw 3 Turn the cover counter clockwise until you can loosen the top screw of the photo diode holder
66. g Procedure 108 Leak Test 109 Using the Cuvette Holder 110 Replacing DCB Board and Firmware 113 DCB Board 113 DCB Firmware 113 Replacing Display Boards 114 Replacing the Leak Interface 115 Leak Sensor Assembly 115 Leak Interface 116 Replacements in the Optical Unit 117 Removing the Optical Unit 118 Replacing the PSC Board 119 Replacing Pre amplifiers or Photodiodes 119 Replacing Grating Assembly Parts 120 Replacing Filter Assembly Parts 122 Replacing Mirrors Beamsplitter and Slits 122 Optical Alignment Procedures 123 Procedure 1 Simple Alignment 123 Procedure 2 Sample Beam Alignment 124 Procedure 3 Reference Beam Alignment 126 Cleaning of Optical Unit Parts 127 Upgrade to GPIB 128 Performance Verification 129 What youneed 129 Preparations 129 Starting a run 130 Scaling Factors 131 6 Service Handbook for 1050 Series of HPLC Modules 11 2001 Contents 6 VWD Parts Information This chapter provides information on parts of the 1050 Vari able Wavelength Detectors 133 Overall Diagram 135 Front Panel Parts 138 Leak Interface 138 Font Panel 139 Optical Unit C 140 Optical Unit C Inner Parts Top 141 Optical Unit C Inner Parts Bottom 142 Grating Assembly 143 Filter Assembly 144 Standard Flow Cell C SST Ti 145 Semi Micro Flow Cell SST 147 High Pressure Flow Cell SST 148 Ultra High Pressure Flow Cell SST 149 Preparative Flow Cell Ti 150 Cuvette Holder 152 Accessories 153 Screws 154 7 VW
67. ge 697 for details 574 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Optical Unit Table 3 Correction factors for 79853C flow cells Path length Path length Correction Flow cell type Cellvolume Partnumber nominal actual factor Standard flow cell 14 ul 79853 60000 8mm 8 00 0 19 mm 8 8 05 Standard flow cell TI 14 ul 79853 6001 1 8mm 8 00 0 19 mm 8 8 00 Micro flow cell 1 ul 79853 60010 5mm 5 00 0 19 mm 5 5 00 Ultra High pressure flow cell 14 ul 79853 60013 8mm 8 00 0 19 mm 8 8 00 Ultra High Pressure Flow Cell Typical applications of the high pressure flow cells are e Hyphenated systems LC MS e Supercritical Fluid Chromatography SFC e Multidetector systems e Narrow bore column applications The main difference between the standard and high pressure flow cells is the design of the window assemblies The high pressure flow cells have different windows seal ring and gaskets see Figure 212 The seal rings support and hold the window and at the same time form the high pressure seal NOTE The gaskets windows and rings are not compatible with the high pressure Cell 79853 60009 OBSOLETE that has been replaced by the ultra high pressure Cellflow cell 79853 60013 See Ultra High Pressure Flow Cell SST on page 697 for details The following materials are in contact with solvents Stainless steel AISI 316 Quartz Kapton polyimide Kapton is a registered tradema
68. h detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications Cuvette Holder y T 658 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Using the Cuvette Holder O Preparation for this procedure are _J Remove the normal flow cell LI Install the cuvette holder in the instrument and perform a wavelength calibration _J Have cuvette with standard available 1 Locate the cuvette holder on the desk O C 1 A 2 Unscrew the bracket 3 Insert the cuvette with the sample into the holder z Light path Clear side Service Handbook for 1050 Series of HPLC Modules 11 2001 659 VWD Maintenance Information Using the Cuvette Holder 4 Replace the bracket and fix the cuvette 5 Install the cuvette holder in the instrument 6 Perform your verification CO 660 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Replacing DCB Board and Firmware Replacing DCB Board and Firmware When performing one of these tasks take care for electrostatic discharge protection to keep the electronics alive DCB Board Note the parameters from 31 SET WL PARAM Disconnect the power
69. hat the grating is not fixed on the shaft with the setscrew Set the Param A 200 using service function CTRL 31 SET A PARAM Activate service function CTRL 40 OTH TEST ON Turn the grating so that the center of the image is on the reference slit a small horizontal and vertical deviation can be accepted Fix the grating with the setscrew De activate and re activate CTRL 40 OTH TEST and check the position of the image on the reference slit If not correct loosen the grating and repeat steps 12 to 14 718 Service Handbook for 1050 Series of HPLC Modules 11 2001 15 16 17 18 19 20 21 22 23 24 25 26 VWD Enhanced Optical Unit Information Replacing the Grating or Grating Motor Do a vertical adjustment with Mirror 4 for precise vertical fit of image on reference slit using the setscrew of mirror 4 through the hole in the optical unit cover see Figure 266 on page 715 Carefully replace the cover of the optical unit Take care for the reference aperture handle De activate CTRL 40 OTH TEST OFF press CLEAR CLEAR and BALANCE Execute CTRL 20 Oth CALIB Activate CTRL 40 OTH TEST ON and readjust the Beam Splitter for ideal horizontal fit De activate CTRL 40 OTH TEST OFF Set A 250 nm Activate CTRL 16 PHOTOCURRENT Optimize the sample readings using the setscrew of mirror 4 through the hole in the optical unit cover see Figure 266 on page 715 Unlock the reference apertur
70. ing light intensity to prevent ADC data overflow will be inserted automatically Select control function 20 0th CALIB ENTER enters function 200step CHANGE displays the current parameter ENTER or CLEAR to continue or to abort 150step 0 000V instrument scans now for maximum from step 150 to 250 201step X XXXV maximum when calibration successfully finished press ENTER 201 step OK press ENTER to keep the new value and to leave function If CALIB FAILURE occurs press CLEAR to keep the old value and to leave function Service Handbook for 1050 Series of HPLC Modules 11 2001 653 VWD Maintenance Information Replacement of Deuterium Lamp Step 3 WL CALIBRATION The instrument scans for maximum light intensity of 656 nm line spectrum of deuterium lamp Enter control function 21 WL CALIBRATION ENTER enters function 656nm 1900step displays the current parameter ENTER or CLEAR to continue or to abort 1875step 0 000V the instrument scans now for for maximum for 100 steps 1902step X XXXV maximum when calibration successfully finished press ENTER 656 1902stepOK press ENTER to keep the new value and to leave function If CALIB FAILURE occurs press CLEAR to keep the old value and to leave function 654 Service Handbook for 1050 Series of HPLC Modules 11 2001 NOTE VWD Maintenance Information Flow Cell Maintenance Flow Cell Maintenance To replace or
71. ion At Power ON During power on the instrument run automatically through different selftest routines If all test are passed the display shows HP 1050 VWD While initializing the CPU checks the response of the Vaccuum Fluorescent Display VFD module If there is no problem SELF DIAGNOSIS IN PROGRESS is displayed If there is no response from the VFD the ERROR lamp will light the buzzer is heard for 2 seconds and is halted 1 Check connection DCB KDI and KDI VFD module 1 Replace VFD Module Ll Replace KDI Board 1 Replace DCB Board 1 Replace DPS The ROM Test calculates the checksum and compares it with a stored value If a differrence is found ERROR LED lights ROM TEST FAILED is displayed and the CPU is halted Otherwise ROM TEST OK is displayed 1 Replace EPROM m Replace DCB During RAM Test firstly every RAM address is uniformly written Then in ascending order each address is tested for contents and then the data is inverted and written Then the same procedure is repeated in decending order with the inverted data This cycle is repeated twice If an error is found RAM TEST FAILED is displayed and further operation is prohibited Otherwise RAM TEST OK is displayed l Replace DCB 612 Service Handbook for 1050 Series of HPLC Modules 11 2001 Display Test L SENSOR TROUBLE POWER FAILURE Table 1 VWD Diagnostic amp Troubleshooting Information Error Messages Before Lamp Ignition
72. istory Product History Since introduction of the 79853C Variable Wavelength Detector in January 1992 following changes have been implemented Prefix Changes Prefix Changes Serial Number Changes Additional Information 3145 J 00101 Start of customer shipments amp empty 3152 J 00263 New amplification factor of reference See Modified side due to high output of new DAD Pre Amplifier Gain on lamps page 734 3152 J 00489 Introduction of DCB firmware revision See SN 01050 055 on 4 22 page 734 3217 J 00603 Introduction of DCB firmware revision See SN 01050 055 on 4 23 page 734 3225 J 00773 Introduction of DCB firmware revision See SN 01050 055 on 4 24 page 734 3225 J 01801 Introduction of PTFE ring in optical See SN 01050 068 on unit page 734 Volume changes on Preparative Flow See Table 138 on page Cell in September 1992 574 and Table 187 on page 698 3225 J 02011 Introduction of new DCB PSC boards See SN 01050 072 on page 734 3323 J 03117 Introduction of new lamp housing 3334 J 03255 Change of manufacturing process for to improve stability photo diodes mounting against humidity Ultra High Pressure cell replaced High Pressure Cell in 1993 Service Handbook for 1050 Series of HPLC Modules 11 2001 729 VWD Additional Information Product History Table 1 Prefix Changes Serial Number Changes Additional Information 3522 J 04305 Enhanced optical unit D
73. ium lamp If the motor assembly has to be exchanged it is necessary to do a compensation of motor tolerances Refer to Replacing Grating Assembly Parts on page 668 Grating Asembly The grating is coated with magnesium fluoride The grating surface should not be touched or cleaned see also Replacing Grating Assembly Parts on page 668 This will destroy the surface and reduce the light reflection Service Handbook for 1050 Series of HPLC Modules 11 2001 581 NOTE VWD Hardware Information Optical Unit Mirrors The instrument contains four mirrors M1 M2 M3 M4 Three of them are spherical type one plane On M2 M3 and M4 the beam height is adjustable Mirror M3 and M4 are identical The mirrors are coated with magnesium fluoride They should not be touched or cleaned see also Replacing Mirrors Beamsplitter and Slits on page 670 This will destroy the surface and reduce the light reflection Slit Assemblies The instrument has two slit assemblies The first slit is located at the light entry into the main optical compartment and focused the light on mirror M3 The second slit is in front of the reference diode Beam Splitter The beam splitter splits the light beam One part goes directly to the sample diode The reference diode gets the other part The height of the light beam is adjustable Refer to Replacing Mirrors Beamsplitter and Slits on page 670 for cleaning 582 Ser
74. ly Refer to Table 191 on page 709 for details Both optical unit versions C and D can be operated with firmware revision 4 24 79853 13005 Due to the modifications the photocurrent readings are about 50 of those of the original C opticals To make them comparable the firmware for the enhanced D version got a new revision and part number 708 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Enhanced Optical Unit Information Part Numbers for Enhanced D Optical Unit Part Numbers for Enhanced D Optical Unit Table 1 Enhanced D Version Part Numbers Part Number Description Comments 79853 60000 Standard Flow Cell backward compatible for details see Table 192 on page 710 79853 69014 Exchange D Optica when D optical should be replaced includes firmware 79853 13000 needs Standard Flow Cell 79853 60000 79853 69015 Exchange D Optica when C optical should be replaced 79853 68110 79853 68111 79853 68112 79853 68113 79853 66508 79853 61109 79853 64605 79853 13000 79853 68746 includes a Standard Flow Cell 79853 60000 and firmware 79853 13000 D Mirror 1 Assembly for D only includes test slit D Mirror 3 4 Assembly backward compatible includes test slit D Beam Splitter for D only includes test slit D Lens Assembly for D only includes test slit Pre amplifier Board REF from C used for SAM
75. m 2 5 V during ignition to 0 V after ignition Service Handbook for 1050 Series of HPLC Modules 11 2001 597 Control of Grating Assembly Control of Filter Assembly Remote Control NOTE Leak Detection VWD Electronic Information Detector Controller Board DCB The wavelength is set by rotating the grating with a 2 phase stepper motor The step angle of the stepper motor rotation 1 step angle 3 75 and is reduced to 1 300 by a gear The stepper motor is controlled and driven by DCB The CPU contrls the motor controller IC that drives the motor Stepper Motor reference position is determined by a plate fitted on the swing arm interrupting a beam of photo interrupter The output signal of the photo interrupter is read by a 8 bit ADC of the CPU The rotation of stepper motor controls the insertion of a diffterent filters into the light path cutoff holmium light reduction The stepper motor is controlled and driven by DCB The CPU controls the motor controller IC that drives the motor The REMOTE connectors communicate start or stop error and not ready signal inputs and outputs For detailed description of remote control refer to the 1050 Service Handbook chapter Common Information When the 79853C VWD is used in a system which is connected via remote the VWD should be switched on as first module Otherwise it may influence other modules at power on The leakage from a flow cell is detected by change of The
76. mation Optical Unit Filter Assembly On the 79853C VWD Figure 216 the Filter Assembly has a three filters 1 installed and is processor controlled It can move into four positions OPEN nothing in lightpath at wavelength lt 370 nm CUTOFF cut off filter in lightpath at wavelength gt 370 nm DARK 0 order calibration filter to reduce the light throughput to the photodiode during grating calibration at 0 order light HOLMIUM holmium filter for grating motor alignment A photo sensor 3 determines the correct position Refer to Replacing Filter Assembly Parts on page 670 for cleaning Filter Assembly 580 Service Handbook for 1050 Series of HPLC Modules 11 2001 Figure 12 NOTE VWD Hardware Information Optical Unit Grating Assembly and Motor The Grating has 1200 lines mm and is directly rotated by the Grating Drive Stepper Motor depending on the wavelength entry The whole range 190 600 nm is equal to 1238 steps 15 5 The step angle of the stepper motor rotation is 3 75 and is reduced to 1 300 by a gear mounted directly to the motor shaft The stepper motor is controlled and driven by the Detector Controller Board DCB The stepper motor reference position is determined by a plate fitted on the motor shaft interrupting a beam of a photo sensor The wavelength calibration of the grating is done at the 0 order light position and at at 656 nm which is the emission line of the deuter
77. ns automatically The initial calibration is done at the factory The lamp can be off but the instrument should be on for some time for stabilizing the instrument to reduce drift Control Function DAC Calibration Key Operation Display Description 44 DAC CALIB Select function ENTER Disconnect SIGNAL Cable and Press START START DAC SCALING Scaling starts SCALING 0 Step 0 of 7 is displayed f0 yyyy pre scaling done for step 0 Now you steps 1 to 7 are done DAC CALIB OK Calibration was successful ENTER 44 DAC CALIB Takes new factors and leaves function DAC CALIB FAILED Calibration was not successful CLEAR 44 DAC CALIB Keeps previous factors when calibratio failed and leaves function 646 Service Handbook for 1050 Series of HPLC Modules 11 2001 45 WL COMPENSATE NOTE Table 24 VWD Diagnostic amp Troubleshooting Information Service Control Functions Wavelength Compensation This control function is used to compensate non lineratity of the grating drive If the grating motor or the grating has been replaced or moved this function MUST be executed During this test the flow cell should be empty no windows or clean filled with water or methanol no air bubbles Absorption in the flow cell causes an error e First step is to search for the 656 nm line emission of the deuterium lamp within a window of 50 steps e Then the processor sets the ideal linear steps for four interpola
78. o 21 are described in detail in the User Documentation Service Handbook for 1050 Series of HPLC Modules 11 2001 621 NOTE VWD Diagnostic amp Troubleshooting Information Service Control Functions Service Control Functions These functions are secured by a PASSWORD because they are normally used by trained Service Engineers Misuse of certain function may result in a misalignment of the optical path or electronical values If the VWD is in service mode the ERROR status lamp blinks If the instrument enters into this mode accidentally the easiest way to abort from this mode is TURN OFF the power off the instrument Entering the Service Mode 1 Press CTRL 3 0 ENTER 2 30 SERVICE MODE ENTER 3 Pass Word 1 0 5 0 ENTER This control function is the entry point for all service control functions You can enter service control function only through this control function Select your desired control function using DOWN or UP Once you abort from this mode you have to execute this function again However if power has not switched off since last entry you can skip password by just pressing ENTER It is adviced that you will turn off the power after you finished using service control functions to avoid the accidental entry to service control mode In Table 151 on page 623 all service related functions are listed Due to different firmware versions and improvements on 79853C VWD the order
79. of the functions is different 622 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 4 CAUTION VWD Diagnostic amp Troubleshooting Information Service Control Functions The functions 20 21 and 22 are part of the User Functions but they are described in this section Service Control Functions Display Used for 20 20 0th CALIB Zero Order calibration 21 21 WL CALIBRATION 656 nm calibration 22 22 HOLMIUMCHECK Wavelength Calibration check 31 31 SET WL PARAM Parameter Set for Zero Order and 656 nm 32 32 FIX SIGNAL Fixed voltage to sample or reference path 33 33 LEAK S VOLT Shows leak sensor voltages 34 34 VOLTAGE TEST Shows DC Voltages 35 35 ADC NOISE Check of ADC noise 36 36 GRATING PS Check of Grating Sensor 37 37 FILTER P S Check of Filter Sensor 38 38 REMOTE TEST Check of Remote lines 39 39 FILTER CHECK Checks movement of Filter 40 40 0TH TEST OFF Positions the Zero Order Beam 41 41 DAC TEST Checks DAC 42 42 PREAMP GAIN Checks linearity of gains 43 43 EEROM TEST Checks EEROM data 44 44 DAC CALIB Calibrates the DAC 45 45 WL COMPENSATE Compensates non lineraties of grating Important parameters might be lost DO NOT use control functions 31 through 45 until you have read this paragraph and fully understand the functions and result of operation Some functions may change the monochrometer parameters and misuse of these functions leads to inaccurate wavelength setting
80. of HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information Service Control Functions Table 5 Control Function Zero Order Calibration Key Operation Display Description DATA UNDERFLOW Indicates that not enough light reaches the sample photo diode Check light path The next line will be shown if no error message occured nn step y yyyV Displays the step that gave the maximum light intensity ENTER nnn step OK Promps for confirmation to change the parameter ENTER Takes the displayed step number CLEAR Rejects the displayed step number ENTER nnn step New parameter is written in EEROM and monochrometer is reset with new parameter 20 0th CALIB End of Zero order beam calibration You may repeat the procedure again Service Handbook for 1050 Series of HPLC Modules 11 2001 625 21 WL CALIBRATION Table 6 VWD Diagnostic amp Troubleshooting Information Service Control Functions Wavelength Calibration This control function is used for the calibration of the monochrometer using the 656 nm line emission from the deuterium lamp The step number appeared in this control function is the number of steps of stepper motor from the 0 order beam to the 656 nm emission line Maximum light intensity is searched between 50 steps of 656 nm value If the maximum peak is found the step number is written into EEROM and corresponds to the value in31 SET WL PARAM Control Function WL C
81. of HPLC Modules 11 2001 619 Table 3 VWD Diagnostic amp Troubleshooting Information User Control Functions User Control Functions These functions are accessable for every instrument user They are used for parameter settings and special operating functions during normal work User Control Functions Display Used for 1 1 SAMPLE SCAN takes a sample scan 2 2 REF SCAN takes a reference scan 3 3 SPECTRUM OUT plots the spectrum 4 4 SCAN FROM defines scan range from 5 5 SCAN TO defines scan range to 6 6 ZERO OFFSET zero offset in 7 7 RESPONSETIME choices 0 25 1 and 4 s 8 8 AUTO LAMPOFF automatic lamp off 9 9 AUTO LAMP ON automatic lamp on 10 10 0UTP DEVICE integrator or recorder 11 11 0UTPUT VOLT 1 V or 100 mV 12 12 STATUS FW revision errors or status 13 13 START MODE local remote hpsystem 14 14 0UTPUT CHECK checks the output voltage 15 15 RESET reset to default 16 16 PHOTOCURRENT sample and reference diode s current 17 17 PARAM LOCK locks certain parameters 18 18 WL SHIFT WL change on display 19 19 POLARITY polarity of signal analog GPIB 20 20 0th CALIB Oth order calibration 620 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 3 VWD Diagnostic amp Troubleshooting Information User Control Functions User Control Functions Display Used for 21 21 WL CALIBRATION 656 nm calibration 22 22 HOLMIUMCHECK WL Calibration check Functions 1 t
82. of both sides separately LI taking the instrument profile of both sides separately With fixed signal either the reference or the sample side is supplied with a constant current So influences from the lamp effects only that side which is not fixed Influence due to a drifting or defective photo diode effects both a scan and the signal noise Small wavelength variations between fixed sample and reference scans are caused by the characteristics of the photo diodes Noise with Reference Signal Fixed If the noise is large the problem may be caused by light path flow cell or sample diode pre amplifier Noise with Sample Signal Fixed If the noise is large the problem may be caused by light path lamp or reference diode pre amplifier To take an instrument profile proceed as follows L Enter 32 FIX SIGNAL and set SMP CH FIXED L Press ENTER and leave function L Press BALANCE L Take areference scan 2 REF SCAN L Take asample scan 1 SAMPLE SCAN 1 Set integrator to ATTN CS10 and Zero10 L Press BALANCE L Enter 3 SPECTRUM OUT press ENTER ENTER 1 Start plot on integrator and press ENTER The intensity profile is now plotted on the integrator Ll Perform above steps with REF CH FIXED to check the light through put of the cell Figure 233 on page 633 shows both plots 632 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information Servi
83. ons 60 4 VWD Diagnostic amp Troubleshooting Information This chapter provides information on error messages and di agnostic features of the 1050 Variable Wavelength Detectors 61 Self Diagnosis 63 During Power On 63 During Normal Operation 63 Error Messages Before Lamp Ignition 64 At PowerON 64 Error Messages After Lamp Ignition 68 Error Messages During Normal Operation 69 Error Messages During Use of Control Functions 71 4 Service Handbook for 1050 Series of HPLC Modules 11 2001 Contents User Control Functions 72 Service Control Functions 74 Entering the Service Mode 74 Zero Order Calibration 76 Wavelength Calibration 78 Wavelength Calibration Check 80 SET WL Parameter 81 Fix Signal 83 Leak Sensor Voltage 86 Voltage Test 87 ADC Noise 88 Grating Photo Sensor 89 Filter Photo Sensor 90 Remote Test 91 Filter Check 92 Zero Order Test 93 DAC Test 94 Pre amplifier Gain 96 EEROM Test 97 DAC Calibration 98 Wavelength Compensation 99 5 VWD Maintenance Information This chapter provides provide procedures for service and main tenance of the 1050 Variable Wavelength Detectors 101 Warnings 102 Securing for Transport 103 Replacement of Deuterium Lamp 104 Step 1 Replacement 104 Step 2 Oth Order Calibration 105 Service Handbook for 1050 Series of HPLC Modules 11 2001 5 Contents Step 3 WL CALIBRATION 106 Flow Cell Maintenance 107 Flow Cell Maintenance Kits 107 Replacing Cell Parts 107 Flushin
84. or 4 through the hole in the optical unit cover see Figure 266 on page 715 Optimize the reference readings with the reference aperture see Optimizing the Reference Readings on page 725 Install the standard slit and perform electronic calibrations see Installing the Standard Slit on page 726 Service Handbook for 1050 Series of HPLC Modules 11 2001 717 VWD Enhanced Optical Unit Information Replacing the Grating or Grating Motor Replacing the Grating or Grating Motor To replace the Grating and or the Grating Motor refer to Replacing Grating Assembly Parts on page 668 and continue with the Alignment Procedure below Install the test slit see Installing the Test Slit on page 713 Carefully replace the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 3 Execute CTRL 20 Oth CALIB 4 Activate service function CTRL 40 OTH TEST ON 5 Carefully remove the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 Unlock the reference aperture see Unlocking the Reference Aperture on page 723 7 De activate CTRL 40 OTH TEST OFF 8 Center the reference slit on the white image by moving the aperture up or NOTE 1 2 6 9 NOTE 10 11 12 13 14 down The image diameter is nearly equal to the reference slit diameter Remove the grating and reassemble new grating Assure t
85. or service and maintenance of the 1050 Variable Wavelength Detectors NOTE WARNING VWD Maintenance Information The information in this chapter is based on the original optical unit version C In June 1995 this optical was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 Warnings Dangerous voltage is present in the cabinet though it is covered and insulated DO NOT TOUCH PARTS unless they are specified in the procedure Be careful when you have to work in the optical unit The lamp housing is hot Be sure to wear a pair of safety or sun glasses Since the deuterium lamp emits intensive ultraviolet light it is dangerous to perform optical alignment without eye protection Be careful not to expose your eyes directly to the light coming from the lamp 650 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information Securing for Transport Securing for Transport Figure 1 Securing for Transport KKK NOTICE xxx PRIOR TO SWITCH ON REMOVE THE SCREWS AND SPACERS ON THE BOTTOM SURFACE OF THE UNIT AS SHOWN IN THE FIGURE BELOW These screws and spacers secure that the optical system is protected from vibration or shock during transportation PROTECTION
86. ore Lamp Ignition During the A D Converter Test the 18 bit ADC for photocurrent acquisition is tested with multiplexer channel fixed at analog ground Pulse count for ground input is measured 20 times and calculate the average and the fluctuations If the value exceeds the pre determined value ADC TROUBLE is displayed 1 Replace DCB During EEROM Data Test various parameters such as monochrometer parameter and time table are stored in EEROM Electrically Erasable Read Only Memory in order to save the value in absence of power At initializing those values are checked using checksum If an error is found EEROM DATA LOST is displayed and default values are set Different types of EEROM DATA LOST messages are possible The key settable parameters wavelength responsetime and so on or time time tables are lost They are replaced by default values LJ Re enter the values m Replace DCB Wavelength parameter zero order are lost They are replaced by default values The monochromater parameters are differrent for each instrument 1 Execute 20 0th CALIB or enter the Oth order parameter directly using 31 SET WL PARAM L Execute 45 WL COMPENSATE The DAC parameters are lost The parameters are the scaling factors for each DAC bit and ZERO SPAN factors The lost parameters are recoverable LJ Execute 44 DAC CALIB The DAC parameters are lost The parameters are the offset parameter of the ADC reading for the output of
87. ormation Performance Verification 680 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information This chapter provides information on parts of the 1050 Variable Wavelength Detectors VWD Parts Information This chapter gives diagrams for parts identification and the complete parts listings respectively e Overall Diagram e Optical Unit e Flowcell e Flow Cell Kits e Accessory Kit NOTE The information in this chapter is based on the original optical unit version C In June 1995 this optical was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 682 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information Overall Diagram Overall Diagram Table 1 Overall Diagram Item Description Part Nmber Item Description Part Nmber 1 Power Supply 5061 3375 01050 69375 15 Cover Assembly 79853 64101 2 DCB Board Assembly see 79853 69511 16 GPIB Board Assembly 79853 68711 2 ROM DCB Firmware C only 79853 13005 16 ROM GPIB Firmware 79853 13004 2 ROM DCB Firmware D only 79853 13000 Cable DCB GPIB 79853 61610 3 PSC Board for DCB 79853 66511 79853 66512 17 Fan Assembly LOW 79853 68503 4 Cable PSC DCB 79853 61605 18 Cover Fan 79853 04102
88. otates the filter 1 step 7 5 to the left DOWN once rotates the filter 1 step 7 5 to the right 638 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information Service Control Functions Remote Test 38 REMOTE TEST This control function is to test the remote control line Disconnect the remote cables to avoid interferrence to and from other instruments Remote lines tested are START SHUT DOWN POWER DOWN READY and STOP PREPARE and START REQUEST are not tested Table 16 Control Function REMOTE Test Key Operation Display Description 38 REMOTE TEST Select function ENTER Disconnect REMOTE Cable and Press START START REMOTE LINE TEST The REMOTE LINE TEST starts REMOTE LINE OK When test is passed REMOTE FAILURE There s a trouble CLEAR 38 REMOTE TEST Leaves function Service Handbook for 1050 Series of HPLC Modules 11 2001 639 VWD Diagnostic amp Troubleshooting Information Service Control Functions Filter Check 39 FILTER CHECK This control function is used to check the motion of second order cutoff filter The cutoff filter returns to original position at the end of this control function Table 17 Control Function Filter Test Key Operation Display Description 39 FILTER CHECK Select function ENTER CUT FILTER OFF Displays present filter status DOWN or UP CUT FILTER ON Cutoff Filter moves in or out CLEAR 39 FILTER CHECK Leav
89. overs over mirror M2 5 Adjust the angle and direction of mirror M2 5 so that the light beam covers over mirror M3 6 Install the entrance slit 3 where the light beam illuminates the center of the slit Adjust the angle and direction of the mirror M3 6 so that the light beam covers over the grating mirror 7 Optical Unit Parts 5 A A OJ FeR J 2 6 LIN 1B e TaZ Il ae l Als 5 s ro e C 7 a8 H o O LI Me al z 10 6 el pA O ee io 9 Jom 7 aA 11 12 8 672 Service Handbook for 1050 Series of HPLC Modules 11 2001 Grating M4 Beam Splitter M4 fine tuning VWD Maintenance Information Optical Alignment Procedures m L O O O O Excute control function 40 0TH TEST OFF to set the grating motor position to 200 steps center step number Adjust the direction of the grating mirror 7 so that the light beam covers over the mirror M4 6 Remove the beam splitter 8 now so that you can see the center of the flow cell Adjust the angle and direction of the mirror M4 6 so that the light beam illuminates the center of the flow cell Leave control function 40 0TH TEST OFF and return to normal display Excute the 20 0th CALIB and 21 WL CALIBRATION Refer to Replacement of Deuterium Lamp on page 65
90. qo FLOP s VOLTAGE MICRO SWITCH Z PROCESSOR ES ES 3 FREQUENCY CLOCK Ls z DIVIDER 24MHz Positive or negative reference voltage is alternately added to the integrator Duty cycles of each reference voltage is controlled by the output of a comparator connected to the output of integrator Input voltage U1 is converted to pulse width so that sum of both reference voltages is balanced with the U1 Clock voltage and ES control this system and determines the period The sum of clock voltage in one cycle is set to zero N A a Pulse width which is proportional to input voltage U1 is counted and is converted into a digital output The A D converter has 18 bits resolution with 25 ms conversion time D A converter combines a Pulse Width Modulator PWM and a 12 bit DAC The lower 4 bits of the DAC pulse width modulated with 12 bit resolution is added to the upper 8 bits of the DAC The upper 8 bits are also modulated by fixed pulse width so that the lower 4 bits balances to LSB of the upper 8 bits pulse width is 15 16 The lower 4 bits are scaled to each bit of the upper 8 bits when using 44 DAC CALIBRATION The output of the 12 bit DAC is passed through a low pass filter and 20 bit resolution analog signal is outputted to the BNC Connector The cutoff frequency of a low pass filter is set at 17 Hz while conversion frequency is 183 Hz 596 Service Handbook for 1050 Series of HPLC Modules 11 2001
91. r J16 Pin Purpose Pin Purpose Pin Purpose Pin Purpose Pin Purpose 1 Photocurrent 2 Photocurrent 3 15V 4 15V 5 Digital GND REF SAMP 6 V 7 A DClock 8 RefVoltage 9 AnalogGND 10 1 2 kHz 10 V 600 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Electronic Information Power Supply DPS A Power Supply DPS A Repair Level Fuses and Exchange DPS A Table 4 Part Numbers for DPS A Item Part Number DPS A Exchange 01050 69375 DPS A New 5061 3375 Fuse for 110 V operation 3 A 2110 0003 Fuse for 220 V operation 2 A 2110 0002 For detailed information on the power supply refer the 1050 Service Handbook chapter 1050 Common Information Lamp Ignition The heater output made by a series regulator is in the pre heating status 2 5 V always After ignition a different output voltage is selected depending on the lamp type used In the 79853C VWD the heater is switched off after ignition Service Handbook for 1050 Series of HPLC Modules 11 2001 601 VWD Electronic Information Keyboard Keyboard The flat keys at the front panel is composed of 6 x 4 matrix Data is read by scanning at Key Display Controller on the Keyboard Display Interface Board Key entry is checked by reading the status of the controller by the CPU at every 10 ms The Key Display Controller also controls LED lamp lighting Figure 10 Keyboard O RUN O LAMP LAMP START STOP ERROR nor reapy ON OFF o0
92. r replacements use part number 79853 13004 0 2X NOT officially released Released for 79853A and 79853C in January 1992 Required for 79853C firmware revision 4 21 and above For replacements use part number 79853 13004 0 2X Service Handbook for 1050 Series of HPLC Modules 11 2001 733 NOTE SN 01050 055 SN 01050 068 SN 01050 072 SN 01050 085 SN 01050 104 SN 01050 107 SN 01050 112 VWD Additional Information Hardware Changes and Service Notes Hardware Changes and Service Notes Modified Pre Amplifier Gain First instruments where shipped with a pre amplifier gain of 100 on the reference side The new deuterium lamps 79883 60002 showed sometimes very high energies which gave very high numbers on the reference side 16 PHOTOCURRENT To prevent an overload the amplification factor was changed to 75 The feedback resistors on the reference side changed from 200 MOhm to 150 MOhm and on the sample side from 100 MOhm to 75 MOhm No revision change was made The modified pre amplifiers started with serial number 3145J00263 Important Service Note Service Note 1050 055 describes the introduction of the DCB firmware revision 4 24 and the problems with previous revisions in more detail This note describes problems with POM ring between lamp housing and optical casting Exchange POM ring against PTFE Ring 53C 79853 24500 This note describes problems with negative baseline jumps on an
93. rating movement filter movement leak detector remote control 590 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Electronic Information Detector Controller Board DCB Figure 3 Block Diagram DCB FILTER GRATING PRE AMPLIFIER POS MOTOR POS MOTO SAMP REF J5 J6 J7 J8 J2 J1 7 GAIN DPS J4 CONTROL CONTROL MULTI LEAK J3 PLEXING DATA PROCESSING FAN J10 Ji FILTERING ADC REMOTE CONTROL LOG CONVERSION N ANALOG i DAC OUT SCALING USER INTERFACE f DCB J9 J12 KEYBOARD DISPLAY HP IB Service Handbook for 1050 Series of HPLC Modules 11 2001 591 CPU ROM RAM EEPROM Clock VWD Electronic Information Detector Controller Board DCB Digital Section A 8 bit 1 chip Microprocessor 7810 is used as CPU The 7810 includes 256 byte RAM 8 channel 8 bit A D Converter 16 bit timer event counter 2 channel 8 bit timer and two 8 bit I O Ports The Data Bus is 8 bit multiplexed with the address The ROM contains the firmware of the the detector and can be exchanged separately In addition to the built in functions the 7810 has 64 Kbytel of external memory address and 32 Kbyte ROM 8 Kbyte SRAM and 2 Kbyte EEPROM are added The EEPROM contains e the time program e the grating calibration constant e D A converter calibration constant e p
94. rews of optical unit top cover plate for cell on the optical body blank plate of cell lock screws M4 12mmlg_ screw 702 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Parts Information Screws Table 15 Screws Location Size Length Type lamp house M4 14mmlg_ screw fan assemblies M4 35mmlg_ screw cell M5 10mmlg screw All screws are plus shaped type Service Handbook for 1050 Series of HPLC Modules 11 2001 703 VWD Parts Information Screws 704 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Enhanced Optical Unit Information This chapter provides information about the enhanced optical unit D NOTE VWD Enhanced Optical Unit Information Since June 1995 the design of the optical unit for the 79853C Variable Wavelength Detector VWD was changed to improve its performance under unstable temperature conditions Together with the enhanced optical unit D the standard flow cell was changed 79853 60000 In this document the term D is used for the new enhanced optical design and C for the original optical design Compatibility This new enhanced optical unit D is fully backward compatible with all 79853C VWDs shipped since January 1992 The new standard flow cell 79853 60000 is backward compatible with the C optical unit Some of the parts for the enhanced optical unit D are not usable in the
95. ription 1 GPIB Interface Firmware ROM GPIB 2 Fan Assembly LOW 3 Fan Assembly HIGH 4 Connection Board PSC 5 Deuterium Lamp Assembly 6 Sample Diode Assembly SDA 6 Pre Amplifier Board SAMP 7 Display Interface Board KDI 7 Display Module VFD 8 Leak Sensor Assembly 9 Reference Diode Assembly RDA 9 Pre Amplifier Board REF 10 Controller Board DCB 11 Firmware ROM DCB 12 Power Supply Assembly DPS A Service Handbook for 1050 Series of HPLC Modules 11 2001 587 VWD Electronic Information Location of Electronic Assemblies Figure 1 Location of Electronic 2 12 11 10 4 a CAUTION WARNING 6 13 g 7 8 588 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Electronic Information Interconnection Diagram Interconnection Diagram Figure 2 Interconnection Diagram gt i M eae o aE E E ET ERa z Optical Unit l D2 Lamp 105V 1 Ea 600V Ly 2 5V ov 1 i Fuses ta da i ea a e a eee EE PENNEN A EEEE a Secondary E o Raiga aa 15V 24 So L lac REF
96. rk of DuPont Recommended pH range 2 3 to 9 5 Service Handbook for 1050 Series of HPLC Modules 11 2001 575 VWD Hardware Information Optical Unit Figure 7 Exploded Diagram of High Pressure Flow Cell i Deuterium Lamp On the 79853C VWD the deuterium lamp Figure 213 is the high intensity lamp 79883 60002 which is same as in the 1040 90 50 series Diode Array Detectors The reduction of energy emission of the lamp Figure 214 on page 577 is time and wavelength dependent and is significantly higher within the first days and for wavelengths in the ultra violet range compared to the visible range change in transmission of lamp glass Usually the response maximum of the lamp is near 230 nm but can be shifted to a higher wavelength for an aged lamp It has no relevance for intensity degradation at other wavelengths The deuterium lamp filament is heated only during the ignition phase The deuterium lamp can be exchanged easily The lamp needs no adjustments 576 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Optical Unit Figure 8 Deuterium Lamp Figure 9 Intensity Degradation of Lamp 79883 60002 100 0 1000 e Measured wavelength is 230 nm e initial intensity about 20 higer than 79880 60002 e decrease in intensity is less with use Service Handbook for 1050 Series of HPLC Modules 11 2001 577 VWD Hardware Information Optical Uni
97. rmal Radiation Constant of NTC thermistor Wheatstone bridge is constructed from NTC thermistor and resistors on the DCB Its supply voltage is varied by controlling 24 V power with Switching Regulator controller The supply voltage is controlled to keep the thermistor at about 150 Ohm 150 C constantly If leakage occurs and the thermistor is soaked in the leaked liquid the thermal radiation constant are changed and gives higher supply voltage at both ends of wheatstone bridge This change is read by the CPU and compensated with ambient temperature Error condition is when LS gt LL The values are visable with 33 LEAK TEST 598 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Electronic Information Detector Controller Board DCB Figure 8 Block Diagram Leak Detection TP1 VOLTAGE EDAN J6 CONTROL 4 LEAK PEE SENSOR 3 E a LEAK SENSOR 1 LLS LEAK LEVEL weston FOR COMPENSATION 2 Change in Thermal Radiation Constant with surrounding temperature change is compensated by second NTC thermistor having same characteristics Service Handbook for 1050 Series of HPLC Modules 11 2001 599 Figure 9 Table 3 VWD Electronic Information Detector Controller Board DCB Board Layout DCB J12 J11 J10 CE ROM J9 ll s y J6 J16 HEH s E Ji J2 J3 pee omit ak Test Connecto
98. rror M3 the grating again a spherical mirror M4 a beam splitter and the flow cell to the sample diode The beam through the flow cell is absorbed depending on the solutions in the cell where UV absorption takes place The intensity is converted to an electrical signal by means of the sample photodiode Part of the light is directed to the reference photodiode by the beam splitter to obtain reference signal for compensation of intensity fluctuation of the light source A slit in front of the reference photodiode focusses the light Wavelength selection is made by rotating the grating which is driven directly by a stepper motor This configuration allows fast change of the wavelength The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light Light Path of Detector i DEUTERIUM LAMP O CAL FILTER CUT OFF HOLMIUM ENTRANCE SLIT FILTER FILTER SPHERICAL MIRROR M1 SAMPLE PHOTODIODE FLOW CELL REFERENCE SA REFERENCE PHOTODIODE 568 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Leak Interface Assembly Leak Interface Assembly To route waste from a module standing above the 1050 VWD to a module below a leak interface can be installed at the detector It is part of the accessory kit Figure 3 Leak Interface O CC NOs miil J Q
99. s 11 2001 725 VWD Enhanced Optical Unit Information Installing the Standard Slit Installing the Standard Slit NOTE This procedure has to be carried out at the end of all replacement procedures 1 Carefully remove the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 2 Remove the test slit from the entrance slit holder using a pair of tweezers and place it safe Figure 10 Replacing the Entrance Slit man entrance slit assembly F test slit mirror 1 assembly A standard slit 3 Carefully insert the standard slit into the entrance slit holder The slit must sit plane on the holder 4 Carefully replace the cover of the optical unit Take care for the reference aperture handle see Figure 266 on page 715 5 Execute CTRL 20 Oth CALIB 6 Execute CTRL 21 l CALIBRATION 7 Reassemble the detector 726 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Additional Information This chapter provides additional information about the 1050 Variable Wavelength Detectors VWD Additional Information Since the introduction of the 79853C Variable Wavelength Detector in 1991 the following hardware and firmware changes have been implemented 728 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 1 VWD Additional Information Product H
100. s are tightened Replace DCB Service Handbook for 1050 Series of HPLC Modules 11 2001 617 VWD Diagnostic amp Troubleshooting Information Error Messages During Normal Operation ADC OVERFLOW 1 Execute BALANCE so that the proper pre amplifier gain is selected m Check whether the flow cell is in correct position and the screws are tightened Is apature gasket installed in flow cell Replace DCB No response for HPIB set address switch correct check connection to DCB and GPIB cable change GPIB board OO CO eo 618 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Diagnostic amp Troubleshooting Information Error Messages During Use of Control Functions Error Messages During Use of Control Functions CALIB FAILURE e When using 20 0th CALIB this message may come up because the lamp is turned OFF or to much light reaches the sample diode M Turn On the lamp LI Reduce light to sample diode e When using 21 WL CALIBRATION this message may come up under the following reasons If during scan the measured voltage of each step is the same Figure 232 a or is on an upslope Figure 232 b or is on a downslope Figure 232 c l Use other wavelength setting to get a different range 31 SET WL PARAM LJ Recalibrate grating with 21 WL CALIBRATION Figure 1 Calibration Failure START STOP SOF SIART X l i i PF AYA ZN START STOP Service Handbook for 1050 Series
101. s from 0 order beam As these values changes slightly from instrument to instrument they are originally calibrated and written into EEROM The parameter 360 8 nm 418 5 nm 486 nm and 536 4 nm cannot be changed These four parameters set with 45 COMPENSATE are used to compensate non linearity of the grating drive If PARM WL656 is changed these four parameters are automatically shifted Control Function SET WL PARAM Key Operation Display Description 31 SET WL PARAM Select function ENTER Parm WLO 200 Displays the 0 order light parameter same value found with 20 0th CALIB UP Select the parameter WL656 nm Parm 656yyyy Displays the step number at 656 nm same value found with 21 WL CALIBRATION Enter the parameter using numeric keys UP Parm 360 8yyyy Displays the step number at 360 8 nm UP Parm 418 5yyyy Displays the step number at 418 5 nm UP Parm 486 yyyy Displays the step number at 486 nm UP Parm 536 4yyyy Displays the step number at 536 4 nm Service Handbook for 1050 Series of HPLC Modules 11 2001 629 VWD Diagnostic amp Troubleshooting Information Service Control Functions Table 8 Control Function SET WL PARAM Key Operation Display Description ENTER 31 SET WL PARAM Control function is displayed again CLEAR On WLO or WL656 parameter reenters the displyed value In order to change both parameters you have to repeat the above procedure for both w
102. sheet Unlocking the Reference Slit handle of reference aperture 0 lock screw 0 reference diode assembly Service Handbook for 1050 Series of HPLC Modules 11 2001 723 nnn a A VWD Enhanced Optical Unit Information Unlocking the Reference Aperture Replace the reference pre amplifier cover to prevent stray light Fit the front panel with one screw at the right of the mainframe Turn on the detector and the lamp Set wavelength to 250 nm Return to your replacement procedure 724 Service Handbook for 1050 Series of HPLC Modules 11 2001 _SaaESSaBDBD9SSSSSS 1 2 3 4 5 6 Figure 9 VWD Enhanced Optical Unit Information Optimizing the Reference Readings Optimizing the Reference Readings Carefully replace the cover of the optical unit Take care for the reference aperture handle Activate CTRL 16 PHOTOCURRENT Shift reference aperture vertically for maximum reference readings Fix the reference slit with the top lock screw of the photo diode assembly Replace the reference photodiode cover Continue with the next step of the procedure of the assembly you are replacing Optimizing the Reference Readings _ fe lt sT handle of reference g 6 aperture L 0 lock screw 0 9 reference diode assembly ame S J a L LI Service Handbook for 1050 Series of HPLC Module
103. solder photodiode LJ unscrew photodiode LI replace photodiode Service Handbook for 1050 Series of HPLC Modules 11 2001 667 VWD Maintenance Information Replacements in the Optical Unit Location of Photodiodes Sample Reference Replacing Grating Assembly Parts Photo Sensor 1 Refer to Removing the Optical Unit on page 666 Ll Remove the bottom plate of the optical unit 1 Unscrew the photo sensor and replace it Grating For items refer to Figure 245 on page 669 1 Remove the top cover of the optical unit 1 Use a hex key 2 5 mm to loosen the grating screw 2 LI Replace the grating 1 1 Refer to Optical Alignment Procedures on page 671 668 Service Handbook for 1050 Series of HPLC Modules 11 2001 Figure 9 VWD Maintenance Information Replacements in the Optical Unit Grating Motor For items refer to Figure 245 on page 669 m m L Refer to Removing the Optical Unit on page 666 Refer to Replacing Grating Assembly Parts on page 668 and take grating out Remove the bottom plate of the optical unit Remove the two springs Unscrew the three screws 6 that fix the motor assembly 5 and take it out Keep spring 3 and gasket
104. t NOTE The lamp should be replaced only if the following two criterias are both fulfilled Baseline Noise with test cell has increased significantly Amount of counts of the lamp with test cell has decreased to less than 50 of the count record of this same lamp when newly installed The decision to replace the lamp due to criterium 2 alone is not relevant because the signal noise may be still within instrument specifications 578 Service Handbook for 1050 Series of HPLC Modules 11 2001 NOTE NOTE Figure 10 VWD Hardware Information Optical Unit Photodiodes Assemblies Two photodiode assemblies Figure 215 are installed in the optical unit The sample diode assembly is located at the right side of the optical unit The reference diode assembly is located in the front of the optical unit Neither the diodes nor the pre amplifier boards are interchangeable Refer to Replacing Pre amplifiers or Photodiodes on page 667 for cleaning Photodiode Assemblies Omm Open oO Li D m O QY O SY 9 L a u N N sample diode reference diode Service Handbook for 1050 Series of HPLC Modules 11 2001 579 NOTE Figure 11 VWD Hardware Infor
105. t the outlet of the flow cell a defined peek capillary from the accessories must be connected to build up a certain back pressure noise reduction See Standard Flow Cell C SST Ti on page 693 and the following pages for parts Service Handbook for 1050 Series of HPLC Modules 11 2001 573 Table 2 NOTE VWD Hardware Information Optical Unit Flow Cell Data STD UHP MICRO STD PREP SST SST SST TI TI Maximum Pressure 40 400 40 40 40 bar Pathlength 8 8 5 8 VAR mm Volume 14 14 1 14 VAR ul Inlet i d 025 0 25 0 10 0 25 0 80 mm Inlet length 555 555 555 555 67 mm Outlet i d 025 025 O25 0 25 0 80 mm Outlet length 67 67 67 67 100 mm Outer diameter 1 16 1 16 1 16 1 16 1 16 inch Used materials for SST flow cells SST quartz and PTFE or Polyimide HP cell Used materials for TI flow cells TI quartz and PTFE STD Standard Flow Cell HP High Pressure Flow Cell replaced by UHP early 1993 UHP Ultra High Pressure Flow Cell replaces HP early 1993 see details on Ultra High Pressure Flow Cell on page 575 PREP Variable Preparative Flow Cell with volume of 0 9 1 8 4 4 or 8 8 ul depending on which gasket is used MICRO Semi Micro Flow Cell The gaskets windows and rings are not compatible with the high pressure Cell 79853 60009 OBSOLETE that has been replaced by the ultra high pressure Cellflow cell 79853 60013 See Ultra High Pressure Flow Cell SST on pa
106. ternal RAM and 32 I O ports 32 Kbyte of ROM for program memory and 128 Kbyte RAM for the run buffer The GPIB board performs all interruption processing from the GPIB controller DCB and GPIB board communicate with hardware interrupts Receiving GPIB commands the GPIB board passes them to the DCB with 3 bit control code This control code shows the kind of bus data such as GPIB commands error codes or requesting data code and whether the data ended or not During run or monitor mode the DCB send chromatogram data immediately to the GPIB board with 8 bit aux code This aux code shows the kind of bus data such as chromatogram data parameters time tables remote line status or GPIB board control code and whether the data ended or not The GPIB board writes the received data into the run buffer The GPIB sends out the formatted data adding the start and the end record The GPIB address setting is done with a switch 1 at the rear of the GPIB board The factory setting is 10 position A Service Handbook for 1050 Series of HPLC Modules 11 2001 607 Figure 14 VWD Electronic Information GPIB Communication Interface Location of GPIB board J12 HPIB Firmware J18 d C WH GPIB Firmware Revisions Refer to GPIB ROM Firmware Revisions on page 733 for information on firmware revisions 608 Service Handbook for 1050 Series of
107. this manual is available as Adobe Acrobat Reader PDF version only and can be downloaded from the Agilent Technolgies web page www agilent com Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Contents 1 VWD General Information This chapter provides general information about the 1050 Variable Wavelength Detectors 11 About the Detector 13 Versions vs Support Periods EOS 13 79853A 13 79853C 13 Repair Policy 14 Specifications 15 2 VWD Hardware Information This chapter provides hardware information about the 1050 Variable Wavelength Detectors 17 Overview 18 Optical System Overview 20 Leak Interface Assembly 21 Leak Sensor Assembly 22 Fan Assemblies 23 Optical Unit 24 Flow Cells 25 Deuterium Lamp 28 Photodiodes Assemblies 31 Filter Assembly 32 Grating Assembly and Motor 33 Mirrors 34 Slit Assemblies 34 Beam Splitter 34 Enhanced Optical Unit D 35 Service Handbook for 1050 Series of HPLC Modules 11 2001 Contents 3 VWD Electronic Information This chapter provides electronic information about the 1050 Variable Wavelength Detectors 37 Location of Electronic Assemblies 39 Interconnection Diagram 41 Detector Controller Board DCB 42 Digital Section 44 Analog Sections 46 Power Supply DPS A 53 Keyboard 54 Keyboard Electronics KDI VFD 55 Pre Amplifier Boards 57 Power Supply Connection Board PSC 58 GPIB Communication Interface 59 GPIB Firmware Revisi
108. ting Ready 01050 90211 Cable Remote 5061 3378 PEEK Waste Accessory Kit 5062 8535 Standard Accessory Kit 79853 68701 includes Fitting 0100 1516 Fuse 250 V 2A 2110 0002 3 Fuse 250 V 3A 2110 0003 3 Wrench 1 4 5 16 inch 8710 0510 1 Screwdriver POZI 1 PT 3 8710 0899 1 Leak Interface Kit 79853 68731 1 Manual SOP 79853 90009 1 Service Handbook for 1050 Series of HPLC Modules 11 2001 701 Table 15 VWD Parts Information Screws Screws Below table lists all screws within the instrument They can be bought locally if needed Screws Location Size Length Type filter motor M2 3 4mmlg hexagon socket set screw with cup point grating motor M3 3mmlg hexagon socket set screw with cup point mirror adjustment M3 8mmlg hexagon socket set screw with dog point grating adjustment M3 14mmlg hexagon socket cap screw grating motor M3 4mmlg countersink screw to fix assemblies on the optical body coveron M3 6mmlg screw the DCB board plate of lamp house KDI and VFD board mirrors filter and grating position sensor M3 6mmlg screw with smaller head REF sensor plate M3 10mmlg screw SAMP and REF amplifier boards M3 18mmlg screw cover assembly optical unit top cover DCB M4 6mmig screw with lock and GPIB boards from rear panel washer to fix assemblies on the base DCB abd GPIB M4 5mmlg screw board leak detector assembly front panel bracket SAMP and REF covers optical unit bottom cover blank sc
109. tive points 360 8 nm 418 5 nm 486 nm and 536 4 nm 360 8 nm and 418 5 nm are absorption points of the holmium oxide spectra 536 4 nm is a line emission from the deuterium lamp e Next it scans each ideal interpolative point 25 steps as reference e Then the holmium oxide filter is inserted and it scans again for the three absorption points of holmium oxide spectra 25 steps e Ifallfound values are in limit then the parameters are written into EEROM visable with 31 SET WL PARAM Control Function WL Compenstation Key Operation Display Description 45 WL COMPENSATE Select function ENTER PARAM CHANGE ENTER to continue calibration CLEAR to abort calibration XXXstep y yyyV displays steps and voltage during calibration CALIB FINISHED new parameter written into EEROM Service Handbook for 1050 Series of HPLC Modules 11 2001 647 VWD Diagnostic amp Troubleshooting Information Service Control Functions Table 24 Control Function WL Compenstation Key Operation Display Description ENTER 45 WL COMPENSATE Takes new factors and leaves function WL CALIB FAILURE one or more peaks could not be found with 25 steps Parameters are set to previous values CLEAR 45 WL COMPENSATE Keeps previous factors when calibration failed and leaves function 648 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Maintenance Information This chapter provides provide procedures f
110. to sensor reaches at backward limit position A normal step value for change from LOW to HIGH is around 200 steps Control Function Grating Photo Sensor Key Operation Display Description 36 GRATING PS Select function ENTER STPxxx PSO 11 Present step of stepper motor and photo sensor voltage is displayed DOWN or UP STPxxx PSy yy You can move the stepper motor and observe the voltage ENTER STP nnn PS3 90 Starts automatically the search for the home position and displays the step number nnn and the corresponding voltage CLEAR 36 GRATING PS Leaves function Service Handbook for 1050 Series of HPLC Modules 11 2001 637 37 FILTER P S Table 15 VWD Diagnostic amp Troubleshooting Information Service Control Functions Filter Photo Sensor This control function can be used to check the function of the photosensor and the filter motor The movement is done step for step It will provide the step number and the voltage of photo sensor when the drive mechanism moves into the photo sensor Control Function Filter Photo Sensor Key Operation Display Description 37 FILTER PS Select function ENTER STPO PS4 98 Move to home position and shows steps and photo sensor voltage DOWN or UP STPxxx PSy yy You can move the stepper motor and observe the voltage When the motor leaves the photo sensor then PS changes to 0 01 CLEAR 37 FILTER PS Leaves function Pressing UP once r
111. vice Handbook for 1050 Series of HPLC Modules 11 2001 VWD Hardware Information Enhanced Optical Unit D Enhanced Optical Unit D In June 1995 this original optical unit was replaced by the enhanced version D to overcome baseline stability problems in unstable environments For details on this D version refer to section Enhanced Optical Unit Information VWD Enhanced Optical Unit Information on page 705 Service Handbook for 1050 Series of HPLC Modules 11 2001 583 VWD Hardware Information Enhanced Optical Unit D 584 Service Handbook for 1050 Series of HPLC Modules 11 2001 VWD Electronic Information This chapter provides electronic information about the 1050 Variable Wavelength Detectors VWD Electronic Information This chapter gives information about the electronic of the 1050 Variable Wavelength Detector e Overview e Interconnection Diagram e Detector Controller Board DCB e Power Supply DPS A e Keyboard electronics e Pre amplifier Boards e Power Supply Connection Board PSC e GPIB Communication Interface 586 Service Handbook for 1050 Series of HPLC Modules 11 2001 Table 1 VWD Electronic Information Location of Electronic Assemblies Location of Electronic Assemblies In the 1050 VWD the following electronic assemblies are available for item numbers refer to Figure 218 on page 588 Electronic Assemblies Item Desc

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