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1. 24 2 OPTICAL 25 271 BELLOWS ADAPTER wececcccccecccnccacceneensesneeaaenneeneeaneeneeaneeneeanenueeusennenueenneauesnaenes 25 2 8 LASER SOURCES ccc ccc ccccceceececceceecencencecencenceceecensenseneeaeacenseneecesaensenseneeneaess 25 PINE S Cc EEE 26 Version 1 1 rev 04 June 2015 2 ANDOR company SECTION 3 INSTALLATION 1 2er erreur nnn nnn 27 3 1 LOCATION AND MOUNTING e cernere nennen nnn 27 3 2 VENTILATION 27 3 3 RR RRRRRRCRRRSRSRRRRRSRRRRRRRRERRRDRR DA 27 3 4 FILTER 27 3 5 CLASSIFICATION OF AN INSTALLED SYSTEM 28 SECTION 4 OPERATION2. 29 4 1 EMERGENCY MAINS DISCONNECTION enn mH nnn 29 4 2 POWER UP 29 4 3 POWER DOWN SEQUENCE 30 4 4 RISK 30 4 4 1 HOUSINGS 30 4 4 2 HAZARDS DUE TO MOISTURE OR LIQUIDS eee eere nnn nnn 30 4 5 USING THE DISKOVERY 31 4 5 1 LCD TOUCHPAD USER INTERFACE eene nnne nennen nnne nnn nnn 31 4 5 2 COMPUTER CONTROL UTILITY APPLICATION GUI ses 33 4 6 DISKOVERY CONTROL 37 4 ADDITIONAL 38 4 7 1
3. eere nnne nnn nnn 42 6 1 2 Access DOOR OPEN ERROR MESSAGE een nnne 42 6 1 3 No LASER POWER AT DISKOVERY enun n rana 42 6 1 4 Low LASER POWER DISKOVERY eere rennen nennen nnne nnn 42 6 1 5 Poor UNIFORMITY OVER CAMERA enne nnne nnne nhe n nenne ann n nua 42 6 2 PROBLEM REPORTING FORM cereum nennen enr 43 SECTION 7 TECHNICAL SPECIFICATIONS een 44 7 1 DISKOVERY GENERAL SPECIFICATIONS ern nnn 44 7 2 BELLOWS 44 7 3 ENVIRONMENTAL SPECIFICATIONS enr mH mmn 44 7 4 POWER SPECIFICATIONS 44 APPENDIX MECHANICAL DRAWINGS eere nnne 45 APPENDIX 46 APPENDIX C OTHER INFORMATION e rrr mmm 47 a Version 1 1 rev 04 June 2015 4 ANDOR
4. the Control Utility application s GUI Ex Filters This button available only on units that possess an excitation filter wheel opens the window shown in Figure 18 and allows the user to select an excitation filter from up to a maximum of 4 Figure 18 Excitation filter wheel screen with sample filter names shown Version 1 1 rev 04 June 2015 32 ANDOR company OPERATION Memory The memory button opens the window shown in Figure 19 and allows the user to save and recall up to 4 different Diskovery configuration settings RECALL FROM oTARTUP Figure 19 Configuration Memory Buttons A 5 2 COMPUTER CONTROL UTILITY APPLICATION GUI The following sections explain how to connect to the Diskovery through the Control Utility application Connection After launching the application a computer connection to the Diskovery Platform must be established through one of the computer s COM ports A right click on the button on the bottom left corner of the GUI will present four options Connection Reset Device TIRF Setup and Help Select Connection ILLUMINATION CONFOCAL EMCCD Full EMCCD 1 3 Connection Reset Device Button Names TIRF Setup Help Figure 20 Connection menu with TIRF Version 1 1 rev 04 June 2015 33 ANDOR company OPERATION Under Port Name select Spectral Diskovery Platform as shown in Figure 21 Select Port Port Name Spectral Diskovery Platform COM
5. 2 5 2 3 2 ON OFF SwitcH A power switch enables power to Diskovery to be switched on or off 2 3 3 USB The Diskovery is controlled by the control PC through the USB 2 0 connection 2 3 4 NTERLOCK OUTPUT An Interlock connection BNC type must be connected to the laser source so that if the Disk Cassette Access Door Section 2 4 3 is opened to exchange discs the Interlock signal is open circuited and the laser source shuts off all lasers In addition the confocal disk stops spinning and is withdrawn from the optical path The installed Diskovery platform includes an interlock system for the Diskovery and microscope that is tied into the laser sources Remote Interlock circuit refer to Section 2 9 2 3 5 FIBRE INPUT The fibre Input Shown unconnected in figure 8 is used for to connect to the Borealis BCU multi mode output This enables the Diskovery to offer both Spinning disk confocal microscopy and Borealis Wide Field BWF modes through this optical connection Version 1 1 rev 04 June 2015 22 ANDOR company 2 4 INTERFACES AND CONTROLS FRONT PANEL There are a number of interfaces on the front panel of the Diskovery these are outlined below Diskovery Manual Dichroic LCD Touchpad LCD Dimmer Disk Cassette Selector Module Control Access Door Figure 12 Front Panel of the Diskovery 2 4 1 LCD TOUCHPAD The LCD screen is located on the front panel and provides touchpad controls to allow ma
6. 2015 41 ANDOR company TROUBLESHOOTING SECTION 6 TROUBLESHOOTING 6 1 TROUBLESHOOTING EXAMPLES The following examples provide guidance for some basic troubleshooting scenarios Refer also to the operation and troubleshooting information provided with your other system components Contact your Spectral or Andor customer support representative if you require further assistance 6 1 1 No EXTERNAL POWER ERROR MESSAGE Error Message No External Power 1 Check to make sure the 12V power supply is securely plugged in 6 1 2 Confocal ACCESS DOOR OPEN ERROR MESSAGE Error Message Confocal Access Door Open Error 1 Check that the Confocal Access Door is closed correctly Message 6 1 8 No LASER Power AT DISKOVERY 1 Check that disk door is closed Door is interlocked to shut lasers off if opened 2 In TIRF mode check that TIRF is enabled 3 In widefield mode check that disk is in OUT position No laser power out of Diskovery 6 1 4 Low LASER POWER AT D ISKOVERY Low laser power at 1 Contact your customer support representative Diskovery PP 6 1 5 Poor UNIFORMITY OVER CAMERA FOV Poor uniformity over Diskovery FOV 1 Contact your customer support representative _ Version 1 1 rev 04 June 2015 42 ANDOR company TROUBLESHOOTING 6 2 PROBLEM REPORTING FORM Please have
7. 60825 1 Beam Conditioning Unit Homogenizes the light from the laser source to permit uniform illumination across the image Confocal Spinning Disk Confocal Laser Scanning Microscopy Direct Current Change of the spatial distribution of a beam of radiation by scattering in many directions by a surface or medium Electromagnetic Compatibility Electrostatic Discharge European Union Fibre Optic connector Angled Physical Contact Input Output Generic input and output electrical signal connections Integrated Laser Engine Control for up to 4 individual lasers ILE 400 and 7 in ILE 700 Primary Secondary configuration All viewing conditions whereby the eye is exposed to the direct or specularly reflected laser beam in contrast to viewing of for example diffuse reflections Light Emitting Diode Laser Merge Module Combines multiple laser wavelengths into a single fibre optic cable output Multi mode fibre Multi mode fibres have a much larger core diameter than single mode SM fibres This enables improved transmission of longer wavelengths Nominal Ocular Hazard Distance Distance from the output aperture at which the beam irradiance or radiant exposure equals the appropriate corneal maximum permissible exposure MPE Maximum Permissible Exposure Level of laser radiation to which under normal circumstances persons may be exposed without suffering adverse effects The MPE levels represent the maximum level to which the eye
8. ANY MAINTENANCE CHECKS e DO NOT USE EQUIPMENT THAT IS DAMAGED e CONTACT YOUR ANDOR REPRESENTATIVE IF THERE ARE ANY QUERIES OR ISSUES WITH YOUR DISKOVERY SYSTEM 5 1 CLEANING AND DECONTAMINATION The most critical aspect of maintenance by the user is to ensure that the system is in a clean environment that is suitable for sensitive electro optical equipment The laboratory should be free of dust fumes and other materials that could affect the system e To clean the product only use a damp lint free cloth on the external housing of the unit Do not wet the connectors Use water only do not use solvents cleaning agents or aerosols 5 2 REGULAR CHECKS The state of the product should be checked regularly especially the integrity of the enclosure and the mains cable On a Daily Basis e Visually inspect the system e Perform any maintenance activities suggested by the microscope and camera manufacturer s On a Weekly Basis Ensure that all power cables are firmly in place e Check the optical cables and connections to ensure that the locks are in place and no damage has occurred to the optical fibres connecting the various elements of the system 5 3 ANNUAL ELECTRICAL SAFETY CHECKS It is advisable to check the integrity of the insulation and protective earth of the product on an annual basis e g U K PAT testing 95 Version 1 1 rev 04 June
9. REMOVING THE DISK CASSETTE weseccsccccccccceccenccnccenecanenneeneeaneeneeanesneeaaeenesnesanesass 38 4 7 2 EXCHANGING A DISK CASSETTE cssescscccnccccceceeacceneenecnneeneeneeaneeneeanesneeanesesaneanesass 39 4 7 3 OPERATING THE DISKOVERY EXCITATION FILTER WHEEL eee eee eee 40 4 7 31 CHECK IF THE EXCITATION FILTER WHEEL IS ACTIVE I E INSTALLED 40 4 7 3 2 TO MOVE THE EXCITATION FILTER WHEEL TO ONE OF THE PRESET FILTER POSITIONS setis RR REFER DOCERE ORDRE KR ERR RA 40 4 7 3 3 TO QUERY THE CURRENT POSITION OF THE EXCITATION FILTER WHEEL 40 Version 1 1 rev 04 June 2015 3 ANDOR company SECTION 5 41 5 1 CLEANING AND DECONTAMINATION eene nnne 41 5 2 REGULAR 41 5 3 ANNUAL ELECTRICAL SAFETY 41 SECTION 6 TROUBLESHOOTING 42 6 1 TROUBLESHOOTING EXAMPLES eee nnne 42 6 1 1 No ExrERNAL POWER ERROR MESSAGE
10. The following test criteria should be applied if a system is felt to be fully enclosed e g uses a stage cover and or environmental enclosure Assess if any of the laser emissions are open to Human Access as defined by 1 Ability of the human body to meet laser radiation emitted by the laser product i e radiation that can be intercepted outside of the protective housing or 2 Ability of a cylindrical probe with a diameter of 100 mm and a length of 100 mm to intercept levels of radiation of Class 3B and below or 3 Ability of ahuman hand or arm to intercept levels of radiation above the AEL of Class 3B 4 Also for levels of radiation within the protective housing that are equivalent to Class 3B or Class 4 ability of any part of the human body to meet hazardous laser radiation that can be reflected directly by any single introduced flat surface from the interior of the product through any opening in its protective housing The standard Ophir PD 300W sensor and power meter can be used to assess accessible power levels in conjunction with suitable Safety Glasses and applicable Safe Systems of Work Any queries should be referred to the Andor Product Laser Safety Officer for guidance lf Human Access is not possible then the device should be reclassified as a Class 1 device This involves the following steps 1 Remove all other Explanatory Classification Labels EXCEPT those on the Laser Sources these count as Removable Laser Sources a
11. a Class Laser Product Left and for a Class 4 Laser Product Right EXPLANATION OF PRODUCT SYMBOLS This symbol found on the power input connection indicates that the Diskovery requires a Direct Current DC power supply Information on the supplied power supply and requirements is detailed in Section 2 This label is located on the Diskovery sled mounting platform to the right of the confocal output This indicates that the Diskovery requires a two person for transport and installation refer to Section 3 Version 1 1 rev 04 June 2015 14 INTRODUCTION SECTION 1 INTRODUCTION ANDOR company This manual provides an overview of the Diskovery and its integration and function as a multi modal imaging system Diskovery developed by Spectral offers several applications within the same package Spinning disk confocal microscopy total internal reflection fluorescence TIRF microscopy and widefield super resolution localization microscopy Thus Diskovery has the following features e Multiple techniques can be used without changing set ups on the same sample Computer or manual control using a LCD touchpad interface display e Flexible bellows attachment to the microscope e Switchable camera lens tubes Diskovery is manufactured by f SPECTRAL Spectral Applied Research Inc A Division of Andor Technology 2 East Beaver Creek Rd Bldg 2 Richmond HIII ON Canada LAB 2N3 Web ww
12. company Revision History Hev A 24 Dec 2014 Initial Release for production 1 0 21 May 2015 Updated presentation and information all sections 1 04 Jun 2015 Updated Figure 8 to show compliance label and serial ID for both Laser Class 3B and Class 4 Diskovery units _ Te Version 1 1 rev 04 June 2015 5 ANDOR company Safety and Warning Information PLEASE READ THIS INFORMATION FIRST CAUTION USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE 1 Ifthe equipment is used in a manner not specified by Spectral or Andor the protection provided by the equipment may be impaired 2 Do not position this product so that it is difficult to operate the Mains disconnecting device See SECTION 4 1 Emergency Mains Disconnection 3 Before using the system please follow and adhere to all warnings safety manual handling and operating instructions located either on the product or in this User Guide 4 Users must be authorised and trained personnel only otherwise this may result in personal injury and or equipment damage and impaired system performance 5 Diskovery weighs over 18 kg 40 lbs depending on system configuration and therefore requires due consideration during manual handling for transport and installation When mounted on the sled the Diskovery and sled weighs 27 Kg 60 Ibs and must be lifted b
13. into an assembled system of components which consists of a laser source a Borealis beam conditioning unit BCU the Diskovery instrument and a research grade microscope The recommended light source integrated separately provides selected laser output s via either single mode or multi mode optical fibres The multi mode fibre is connected to a Borealis BCU integrated separately the output of which is connected to one input of Diskovery The Diskovery is intended to provide three imaging modes in one instrument Spinning disk confocal microscopy using the multi mode input from the BCU Borealis Wide Field BWF using the multi mode input from the BCU Total internal reflection fluorescence TIRF microscopy via single mode input from the laser source Figure 9 shows the Diskovery without the laser source or the BCU Figure 9 The Diskovery shown with Ultra EMCCD and Zyla sCMOS cameras attached Version 1 1 rev 04 June 2015 20 ANDOR PRODUCT OVERVIEW company 2 2 SYSTEM CONFIGURATIONS The Diskovery instrument is configured with BWF and Confocal capability The instrument can be upgraded for additional TIRF capability Thus there are two models available CR DISK CONF containing BWF and DISK CR DISK TIRF consisting of the TIRF module as an upgrade to the above The Diskovery instrument is usually mounted onto a support sled so that a camera can be mounted for the horizontal out
14. is only likely if the beam is a small focused spot Note There exist some theoretical but rare viewing conditions where viewing a diffuse reflection could exceed the Maximum Permitted Exposure MPE For example for Class 3B lasers having powers approaching the AEL lengthy viewing of greater than 10 s of true diffuse reflections of visible radiation and viewing at distances less than 13 cm between the diffusing surface and the cornea can exceed the MPE Laser products for which intrabeam viewing and skin exposure is hazardous and for which the viewing of diffuse reflections may be hazardous These lasers also often represent a fire hazard Version 1 1 rev 04 June 2015 9 ANDOR an Oxford Instruments company LASER CLASSIFICATION LABELLING LOCATIONS The Access Panel and Classification Explanatory Labels of the Diskovery are located as shown If the Diskovery is to be re classified by the site s trained and competent individual e g the site s Laser Safety Officer then they must ensure that labels of the correct classification and wavelength range are placed in all of the locations indicated below A further explanation of these labels is described in the following sections Figure 2 Camera 2 Panel Label Attached if Second Camera is not Present Diskovery Top View Version 1 1 rev 04 June 2015 10 NDOR company Figure 3 TIRF Housing Panels Diskovery Top View Figure 4 TIRF Blanking P
15. pushed to adjust the penetration depth to 120 nm The user can adjust the penetration depth by dragging the penetration depth slider OR by using the drop down box If the drop down box is used the Go button must be pushed to make the change The Hi Resolution button changes the penetration depth step size from 10 nm to 1 nm 488 rm 561 nm Hi Resolution New Depth nm 120 Current Depth nm Figure 26 TIRF Menu wavelengths selected and penetration depth set The last control on the screen is the Oblique Illumination button shown in Figure 26 Selection of this option takes the system out of TIRF and into sub critical angle oblique widefield illumination V 488 rm 561 nm Hi Resolution New Depth nm 120 Current Depth nm Figure 27 TIRF Menu Oblique Illumination Dirty TIRF Selected To exit the TIRF control the user should push the return button shown in Figure 27 Version 1 1 rev 04 June 2015 36 ANDOR company OPERATION 4 6 DISKOVERY CONTROL ABOUT The About screen gives information for the Diskovery including serial number and the software hardware and firmware versions Model DISKOVERY Part Diskovery Platform Serial 05 52013 Diskovery Graphical User Interface Control Application For technical support or problems with this application please contact 1 305 326 5040 Copyright 1989 2014 Spectral Applied Rese
16. 39 ANDOR company OPERATION 4 3 OPERATING THE DISKOVERY EXCITATION FILTER VV HEEL The Diskovery Platform uses a standard 4 line ILE laser engine which usually includes 405 nm 488 nm 561 nm and 638 nm lasers Occasionally the Diskovery Platform may be used with a non standard ILE laser engine configuration that includes additional or unconventional lasers with different wavelengths such as 440 nm 514 nm 730 nm etc thus necessitating the application of a motorized excitation filter wheel built into the Diskovery unit that carries various laser clean up excitation filters In this situation the combined activation of various lasers within the laser engine must be coordinated with the filter switching action of the excitation filter wheel which can be achieved by one of two methods e First as various laser are activated the excitation filter can be switched manually by the user by accessing the filter wheel position via the Diskovery Platform LCD touchpad interface screen or the Diskovery Platform computer control application See Figure 16 e Second the excitation filter wheel can be switched via RS 232 serial communication through the image acquisition software In this case the system installer must define the excitation filter wheel position for each image channel so that the filter wheel will automatically switch to the appropriate excitation filter when a certain combination of lasers is activated Further informatio
17. ANDOR an Oxford Instruments company Diskovery Version 1 1 revised 04 June 2015 User Guide andor com Andor Technology 2015 ANDOR company TABLE OF CONTENTS SAFETY AND WARNING INFORMATION 6 SECTION 1 15 1 1 TECHNICAL 16 1 2 17 1 3 COPYRIGHT AND PROTECTIVE NOTICES ern 17 1 4 TRADEMARKS AND PATENT INFORMATION err 17 1 5 SUPPLIED enne nennen nennen nnn rennen 18 1 06 OPTIONAL COMPONENTS 19 SECTION 2 PRODUCT OVERVIEW crecen erre nnne nene nra 20 2 1 GENERAL 20 2 2 SYSTEM 21 2 3 INTERFAC
18. AS IS WITHOUT WARRANTY CONDITION OR REPRESENTATION OF ANY KIND EITHER EXPRESS IMPLIED STATUTORY OR OTHERWISE INCLUDING BUT NOT LIMITED TO ANY WARRANTY OF MERCHANTABILITY NON INFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL ANDOR BE LIABLE FOR ANY LOSS OR DAMAGE WHETHER DIRECT INDIRECT SPECIAL INCIDENTAL CONSEQUENTIAL OR OTHERWISE HOWSOEVER CAUSED WHETHER ARISING IN CONTRACT TORT OR OTHERWISE ARISING OUT OF OR IN CONNECTION WITH THE USE OF THE INFORMATION PROVIDED HEREIN 1 3 COPYRIGHT AND PROTECTIVE NOTICES The copyright in this document and the associated drawings are the property of Andor Technology and all rights are reserved This document and the associated drawings are issued on condition that they are not copied reprinted or reproduced nor their contents disclosed The publication of information in this documentation does not imply freedom from any patent or proprietary right of Andor Technology or any third party 1 4 RADEMARKS AND PATENT INFORMATION opectral Applied Research Inc is a Division of Andor Technology Andor and the Andor logo are trademarks of Andor Technology Andor Technology is an Oxford Instruments company All other marks are property of their owners Changes are periodically made to the product and these will be incorporated into new editions of the manual New releases of the manual are available through MyAndor http my andor com login aspx Version 1 1 rev 04 June 2015 17 A
19. ES AND CONTROLS LEFT SIDE PANEL 22 2 31 POWER 22 2 3 2 ON OFF 22 PAS e NSO cease dade 22 2 3 4 INTERLOCK 22 2 99 FIBRE NPUT pe RR 22 2 4 INTERFACES AND CONTROLS FRONT PANE L cccccecceceeceecsceeees 23 240 T LCD E ERE 23 2 4 2 MANUAL DICHROIC SELECTOR MODULE 23 2 4 3 Disk CASSETTE ACCESS DOOR uiscscsccscccccnccnescecceneenenceccenesneseeneenesneseeneenennesseas 23 25 POWER SUPPLY MEE pM 23 26 DISKOVERY SYSTEM INTEGRATION mH nmn 24 2 6 1
20. Figure 21 Connection Select Port On the same menu as the Connection button is a Reset Device button You may be asked to reset the device during a remote troubleshooting session with a service engineer Diskovery Control This action will reset the device and initiate a calibration cycle Is this action required Figure 22 Reset Device Figure 23 shows the main screen once with the device connected It is almost identical to the Diskovery Platform LCD touchpad user interface main screen All buttons except for the TIRF button have been described above If the TIRF is button is selected TIRF control panel Figure 24 is shown CONFOCAL Figure 23 Main Screen E 6 8 Version 1 1 rev 04 June 2015 34 ANDOR company OPERATION Hi Resolution New Depth nm 120 Current Depth nm Figure 24 TIRF Menu Figure 25 shows the screen with the prisms inserted The user should now select the TIRF wavelength or wavelengths Hi Resolution New Depth nm 100 E Current Depth nm Figure 25 TIRF Menu no wavelengths selected MM M 2 e 9 Version 1 1 rev 04 June 2015 35 ANDOR company OPERATION Figure 26 shows two wavelengths selected and the Go button has been
21. NDOR company INTRODUCTION 1 5 SUPPLIED COMPONENTS Diskovery CR DISK CONF Diskovery with Confocal Bellows Adapter Microscope specific Camera Lens Tube Camera specific Power Supply with country specific power cord Hardware Guide in electronic format 1 Diskovery may be ordered with TIRF option see Section 1 6 2 Diskovery may be ordered and configured for 2 cameras and emission filter wheels see Section 1 6 3 Additional camera Lens Tubes may be supplied as ordered see Section 1 6 Version 1 1 rev 04 June 2015 18 ANDOR company INTRODUCTION 1 6 OPTIONAL COMPONENTS There are a range of optional components available for the Diskovery system including e Andor Ultra 897 888 EMCCD or Zyla and Neo sCMOS scientific cameras ASI FW 1000 High Speed Filter Wheel 8 position e 3D Camera Lens for Super Resolution e Bellows Adapter for Leica Auto focus microscope e Additional Confocal Disk and storage box Please contact your nearest Andor Sales Representative for further information on these and other system components available SS TT Version 1 1 rev 04 June 2015 19 ANDOR PRODUCT OVERVIEW company SECTION 2 PRODUCT OVERVIEW The Diskovery instrument is a multi modal imaging platform that is used with a research grade microscope to image biological samples for research applications 2 1 GENERAL The Diskovery product is intended to be integrated
22. PERSON LIFT ONCE INSTALLED AND SECURED TO THE OPTICAL BENCH IT SHOULD BE NECESSARY TO MOVE OR ADJUST THE MOUNTING OF THE DISKOVERY OR OTHER COMPONENTS IF THIS IS REQUIRED PLEASE CONTACT YOUR ANDOR REPRESENTATIVE FOR FURTHER INFORMATION e ANY INSTALLATION STEPS INVOLVING LASERS MUST BE PERFORMED BY QUALIFIED PERSONNEL USING PERTINENT LASER SAFETY PROTOCOLS e SETUP AND OPERATION OF OTHER SYSTEM COMPONENTS ARE DESCRIBED IN THEIR RESPECTIVE GUIDES AND MANUALS 3 1 LOCATION AND MOUNTING e Temperature and humidity must meet the specifications defined in SECTION 7 e Operational vibrations should be reduced as much as possible for stability of the imaging train e tisrecommended that the Disokvery is mounted on a damped optical table Please contact your Andor Representative for assistance in specifying and ordering a suitable table e Power cabling and control cables should be routed to prevent accidents damage and accidental unplugging while avoiding bend radii of less than 30 mm 3 2 VENTILATION Do not cover equipment during operation 3 3 ASSEMBLY This product requires no assembly by the user and is recommended to be installed by an installation engineer authorized by Spectral Andor or authorized distributor 3 4 FILTER WHEELS One or two emission filter wheels are generally provided with the Diskovery These will be installed by the technician and locked in place These devices are not user accessible If it is ne
23. Please refer to section 2 5 for guidance on the assessment and classification procedure Version 1 1 rev 04 June 2015 8 ANDOR company LASER SAFETY INFORMATION All relevant safety compliance information is visibly displayed on the product as per the International Laser Safety Standard IEC 60825 1 and the U S Laser Product Performance Standard 21 CFR 1040 10 As the Diskovery system will form part of a unique configuration of components it cannot be classified in isolation Therefore when integrated it may form part of a system with one of the classifications described below A label in the format depicted below will be included for application by the System Integrator following assessment and classification The Diskovery may be configured as part of a larger system that includes multiple Class 3B and Class 4 lasers A number of separate emission wavelengths can also be available in any one system In some very rare occasions another classification label may be used this will be explained in additional documentation as appropriate Class 3B Laser products that are normally hazardous when intrabeam ocular exposure occurs i e within the Nominal Ocular Hazard Distance NOHD including accidental short time exposure Viewing diffuse reflections is normally safe Class 3B lasers which approach the Accessible Emission Limits AEL for Class 3B may produce minor skin injuries or even pose a risk of igniting flammable materials However this
24. UCT OVERVIEW company 2 9 INTERLOCKS The laser safety interlock system connects the laser source e g ILE to the Diskovery and the microscope in series as shown in Figure 15 The laser source provides a small current to test if the interlocking circuit is complete The interlock will cut power output from the lasers if the Disk Cassette Access Door Sections 2 4 3 and 4 7 1 is opened or if an open circuit is detected in any other part of the interlock system Microscope interlock connections shown below depend on the model but typically include interlock connections for the eyepieces and microscope arm Microscope Interlock connections microscope arm and eye pieces 6 06 ILE Interlock Diskovery Interlock Microscope Connection Connection Rear Panel Left Side Panel Figure 15 Outline of Interlock connections for the laser source Diskovery and microscope Version 1 1 rev 04 June 2015 26 ANDOR company INSTALLATION SECTION 3 INSTALLATION WARNINGS e THE DISKOVERY SYSTEM MUST BE INSTALLED BY AN AUTHORIZED INSTALLATION ENGINEER ACCORDING TO THE INFORMATION PROVIDED BY SPECTRAL OR ANDOR e THE DISKOVERY WEIGHS OVER 18 KGS 40 LBS DUE CARE MUST BE TAKEN WHEN LIFTING THE DISKOVERY AS OUTLINED IN THE ANDOR INSTALLATION AND SERVICE MANUAL WHEN MOUNTED TO THE SLED THE TOTAL WEIGHT OF THE DISKOVERY AND SLED IS 27 KGS 60LBS AND REQUIRES A TWO
25. anel If TIRF Module is not present Figure 5 Diskovery Right Hand Side Locations of Laser Classification and Explanatory Labels Version 1 1 rev 04 June 2015 11 ANDOR company CAUTION CLASS 38 LASER RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM Diskovery ANDOR e Figure 6 Diskovery Front Panel Locations of Laser Classification and Explanatory Labels APERTURE The System Integrator MUST ensure that the final system s Laser Aperture is suitably labelled e g the microscope s Objective is identified by a label on the microscope s stage top An example is shown below The appropriate aperture label is included with the documentation for application by the System Integrator ifi LASER APERTURE A Laser emission from microscope objective Figure 7 Example of Laser emission warning label on microscope DESCRIPTION OF EMITTED RADIATION FROM THE LASER PRODUCT Notes Wavelengths 400 785 nm Exact outputs will depend on the integrated laser source please refer to it s Explanatory Label and User Documentation Beam Divergence 0 3 1 4 NA Exact divergence will be dependent on the objective in use please refer to the microscope s User Documentation Maximum Power or lt 1W The maximum output power will depend on the integrated laser source and the configured optical elements but will be significantly Syll CUMS less than 1W Class 4 Pulse Duration N A All recommended laser sources for the Borealis Product a
26. arch Inc All Rights Reserved SW Version 1 0 1 0 JA Spe Research FW Version 000 000 031 HW Version 2 0 2 Figure 28 The Diskovery About Screen Version 1 1 rev 04 June 2015 37 ANDOR company OPERATION 4 ADDITIONAL PROCEDURES The Diskovery Platform confocal pinhole disk cassette is designed to be user exchangeable with other disk cassettes permitting the user to image their microscopic specimens with varying degrees of confocality resolution and image signal strength WARNING e When handling an unmounted disk cassette avoid contact with the transparent cassette optical windows e The confocal pinhole disk cassette should only be accessed on a Diskovery Platform system that has been properly interlocked 4 1 REMOVING THE DISK CASSETTE To remove a confocal pinhole disk cassette from the Diskovery Platform perform the following steps 1 Turn off the lasers 2 Push the exchange button on the LCD or GUI and wait until the display indicates that the disk has finished moving to the exchange position 3 Pull down the confocal disk cassette access door Figure 29 to access the disk cassette Figure 29 Opening Confocal Door to Access Cassette Note If the access door is opened while the disk is moving it will automatically stop The door must be closed again to allow the disk to finish moving to the exchange position 4 Insert a 3 mm hexagonal Allen driver into the confocal disk casse
27. ave an established system for the safe use of lasers as per their national frameworks and Occupational Health and Safety legislation Laser Safety standards IEC 60285 1 and American National Standard Z136 1 2007 Safe use of Lasers may be useful references for good practice ASER PRODUCT CLASSIFICATION The Diskovery does not contain any embedded Laser Sources but it is intended to be integrated with a compatible Laser Source and has therefore been designed to safely accept and handle Laser Radiation The Diskovery unit and all ancillary components of the Diskovery system have been designed and labelled to be compliant with IEC 60825 1 and CDRH Title 21 CFR 1040 10 When assembled and integrated in the intended manner Diskovery will form part of a unique laser product formed by the entire system The Classification of this product will depend entirely on the Laser source connected and the associated research grade microscope Therefore the final assemblage must be assessed Classified and labelled in accordance with the requirements of IEC 60825 1 and CDRH Title 21 CFR 1040 10 This must be performed by a competent trained individual e g the site s Laser Safety Officer In the case of the System being installed by an Andor representative it is Andor s responsibility to assess and classify the final system The Customer Support Engineer will be competent and trained to perform this and ensure that the system is correctly classified and labelled
28. cessary to change or add a filter please contact the nearest Andor representative Note that only certain filters have been certified to date for Diskovery Version 1 1 rev 04 June 2015 27 ANDOR company INSTALLATION 3 5 CLASSIFICATION OF AN INSTALLED SYSTEM The following information provides a reference on how Andor classify a system which is configured with a number of system components one of which is a laser a source prior to installation Refer also to the Laser Safety Information Section in the preface of this manual The proposed installation scheme of all systems is captured and assessed for all orders received by Andor It is the responsibility of the System Integrator to ensure that the final assembled product is assessed and correctly classified and labelled according to IEC 60825 1 or the regional equivalent This assessment must be conducted by a trained and competent individual e g the site s Laser Safety Officer Most devices are classified as 3B during factory QC due to the known and measured powers from the selected laser source If the laser source is not provided by Andor and is of high power gt 500mW then the assembled system should have its maximum accessible emissions measured at the microscope s nose piece with no objective present The device documentation will include a Classification Label sheet which includes all required variations of the labels to allow reclassification of the system if required
29. de fibre via the BCU to the Diskovery BWF input and optionally via single mode fibre to the TIRF module if present where is is processed by the Diskovery The output of the Diskovery is delivered to a research grade microscope using coupling optics The fluorescence emission light returning from the microscope sample is returned to the Diskovery via the same path The Diskovery directs this emission light to the camera s after passing through an emission filter loaded in a manual paddle or a motorized filter wheel Figure 8 below provides a simplified schematic of the Diskovery Platform integrated as a larger system ILE Single mode Fibre option Microscope Multi mode Fibre Diskovery TIRF Input Excitation TT ME HU Emitted 3 Filter Wheel Emitted Support Sled with Emission Filter PHOreseenee Figure 13 Overview of the Diskovery integrated into a typical system 8 e 9 Version 1 1 rev 04 June 2015 24 ANDOR company PRODUCT OVERVIEW 2 7 OPTICAL PATHS An appropriate wavelength and power level for the specific experiment e g using a fluorescent probe is set for the ILE Laser combiner The light of the required wavelength and power passes to the Diskovery normally via the Borealis BCU The light is processed by the Diskovery as r
30. ded minimum bend radius is 30 mm Optical fibres are prone to damage by bending local to the connector The coupler is not designed to withstand pulling of the fibre If the fibre is pulled the system performance could be compromised or the system may fail SERIAL AND COMPLIANCE LABEL The label below is located on the rear panel of the Diskovery It indicates compliance with the applicable standards It also provides information on the manufacturer and identifies the model and its laser classification serial number and manufacturing date month year Spectral Applied Research Inc A Division of Andor Technology Model CR DSK CONF 4 2 East Beaver Creek Rd Blda 2 Serial No D8K52030 Richmond Hill ON L4B 2N3 Canada December 2014 905 326 5040 www spectral ca Spectral Applied Research Inc RES A Division of Andor Technology Model CR DSK CONF 3B 2 East Beaver Creek Rd Blda 2 Serial No D8K52030 Richmond Hill ON LAB 2N3 Canada December 2014 905 326 5040 www spectral ca This product canmpolies with IEG amp O0825 1 2007 and 21 CFR 1040 10 and 1040 11 except for deviations pursuant to Laser Notice Mo 50 dated 2007 06 24 L and 21 OFR 1040 10 and 1040 11 except Tor emi deviations pursuant to Laser Notice No 50 4 pa dated 2007 06 24 7 Figure 8 Compliance and Serial Identification labels on rear panel of the Diskovery Example shown for a Diskovery unit classified as
31. eable parts inside the product and the enclosure must not be opened Only authorised service personnel may service this equipment 23 Do not wet or spill liquids on the product and do not store or place liquids on the product 24 f spillage occurs on the product switch off power immediately and wipe off with a dry lint free cloth 25 f any ingress of liquids has occurred or is suspected unplug the mains cables do not use and contact Andor Customer Support 26 See SECTION 5 1 Cleaning and Decontamination 2 Do not expose the product to extreme hot or cold temperatures 28 Do not expose the product to open flames 29 Do not allow objects to fall on the product 30 Keep this User Guide in a safe place for future reference Version 1 1 rev 04 June 2015 7 ANDOR company LASER SAFETY CAUTION USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE This product is designed to be integrated with lasers that cover the entire visible soectrum and extend into the invisible infra red spectrum This section of the manual is designed to make the end user aware of the hazards of the product due to these lasers Laser safety hazards differ from those of normal light sources and users must be familiar with the hazardous properties of lasers as these are highly concentrated low divergence beams of radiation Andor recommend that all facilities h
32. epresented in Figure 14 and the output of the Diskovery is then delivered to the microscope via coupling optics The reflected scattered or radiated emission light emitted from the microscopic specimen typically living biological cellular samples re enters the Diskovery and is directed to one or two cameras typically EMCCD or sCMOS types The fluorescent light generated by the sample returns to the Diskovery where it is imaged onto the camera s Residual laser light is filtered along the return path using appropriate interference filters typically OD6 or greater loaded into a manual paddle or an emission filter wheel situated before the camera Beam Shaping TIRF Fibre In optics and Excitation Filter Wheel Fibre In for Spinning Disk Chromatic and Widefield Compensation Optics Spinning Disk In or out of path Dichroicless Beam Merge 1 2 Cameras Path to Removable Emission Microscope Filter Wheel Figure 14 A representation of the Optical Paths of the Diskovery 2 1 ADAPTER The bellows adaptor facilitates strain free alignment and connection of the Diskovery to the microscope Adaptors are specific to the microscope model 2 8 LASER SOURCES The Diskovery is compatible with a wide variety of laser sources including the Andor ILE and ALC 601 laser combiners Refer to the information supplied by the manufacturer for each laser source Version 1 1 rev 04 June 2015 25 ANDOR PROD
33. h 4 Confirm that the Emission LEDs turn on Version 1 1 rev 04 June 2015 29 ANDOR company OPERATION 5 Turn on laser source according to manufacturer s instructions Note that the ILE has an ON OFF switch at the rear of the unit and the ON OFF key on the front panel SESS EUR EE UTERE i ON OFF Switch on ON OFF ILE rear Key switch panel on ILE front Panel 6 The system can now be used as outlined in the user guides 7 Refer also to your user guides supplied with your image acquisition software e g Andor iQ 4 3 POWER DOWN SEQUENCE In order to turn the system off proceed as follows 1 Exit the control software Turn off the laser source 2 3 Turn off the Diskovery using the rocker switch 4 Turn off the BCU using the front panel toggle switch 5 Power off the control PC workstation 4 4 Risk MITIGATION 4 4 1 MECHANICAL HOUSINGS Once installed the Diskovery Platform microscope filter wheel s and camera s form the protective housings of the product No components panels connections or linkages should be loosened or removed to avoid exposure to hazardous radiation 4 4 2 HAZARDS DUE TO MOISTURE OR LIQUIDS Do not put any components including power cables or external power supply in places with high moisture or near water Version 1 1 rev 04 June 2015 30 ANDOR company OPERATION 4 5 USING THE DISKOVERY There are two ways to control the Diskovery 1 Using the Disko
34. ks emission LEDs and other safety features Ensuring that all interlocks are connected and functioning correctly We recommend that a copy of IEC 60285 1 is purchased by the laser safety officer for reference GUIDELINES FOR SAFE OPERATION OF LASER PRODUCTS 1 2 3 Read the safety instructions supplied with all equipment in the system Never look into a laser beam either directly or indirectly Do not attempt to disassemble the unit housing the lasers or any part of the system If there is a problem please contact Andor directly see SECTION 1 1 Help and Technical Support Restrict and control access to the area s where are in use to those persons who are trained in the dangers of lasers and trained on the safety precautions to be observed when working with lasers Ensure suitable laser warning signs are prominently displayed in the area the system operates If the system is not in use turn the laser off using the key switch On a daily basis or before every use verify that the laser interlock circuit is working by confirming that the laser emission indicator on the source turns off when any of the following are done e The microscope binocular eyepieces are in the open position e The articulated transmitted light arm on inverted microscopes is tilted back from the functional vertical position before using the system If it is necessary to visually locate and indicate the output of invisible wavelength
35. n is available in Diskovery Command Set Document SU DSK502 4 7 3 1 CHECK IF THE EXCITATION FILTER WHEEL IS ACTIVE I E INSTALLED Send the following serial command to the instrument ACTIVE FILTER The device will respond with ACTIVE FILTER Wzn n 0 Inactive n 1 Active 4 f 3 2 MOVE THE EXCITATION FILTER WHEEL TO ONE OF THE PRESET FILTER POSITIONS Send the following serial command to the instrument A PRESET FILTER Wn 0 lt lt 5 4 7 3 3 QUERY THE CURRENT POSITION OF THE EXCITATION FILTER WHEEL Send the following serial command to the instrument Q PRESET FILTER W The device will respond with PRESET FILTER Wzn 0 lt lt 5 These serial commands are supported from 0 0 31 of the Diskovery firmware The excitation filter identities and their loaded positions in the excitation filter wheel can be determined by observing the names and their ordering in the Ex Filter window of the Diskovery LCD touchpad interface screen or the Diskovery Platform control application see Figure 16 Alternatively the excitation filter identities and loaded filter wheel positions are documented in the shipping records that are delivered with each Diskovery Platform unit Version 1 1 rev 04 June 2015 40 ANDOR company MAINTENANCE SECTION 5 MAINTENANCE WARNINGS e THERE ARE NO USER MAINTENANCE PROCEDURES REQUIRED FOR THE DISKOVERY PLATFORM e THE SYSTEM SHOULD BE POWERED DOWN PRIOR TO USER PERFORMING
36. nd need to remain labelled and classified as stand alone products 2 Fita Class 1 Explanatory Label onto a permanently affixed surface which is easily visible before and during operation of the system 3 Ensure a Laser Hazard Symbol is clearly visible before and during operation and affix a label if not _ _ lt lt E Version 1 1 rev 04 June 2015 28 ANDOR company OPERATION SECTION 4 OPERATION WARNINGS e IF THE EQUIPMENT IS USED IN A MANNER NOT SPECIFIED BY SPECTRAL OR ANDOR SYSTEM DISTRIBUTORS THE PROTECTION PROVIDED BY THE EQUIPMENT MAY BE IMPAIRED e READ THE USER GUIDES SUPPLIED WITH YOUR SYSTEM COMPONENTS AND SOFTWARE PRIOR TO USE 4 1 EMERGENCY MAINS DISCONNECTION In case of emergency the disconnecting point of the equipment is the mains power cord connected to the external power supply or the mains socket switch WARNING SWITCH OFF THE POWER AT THE MAINS SOCKET AND REMOVE THE MAINS LEAD FROM THE EXTERNAL POWER SUPPLY 4 2 PowER UP SEQUENCE 1 Turn on and log in to the control PC workstation 2 Turn on the Diskovery using the rocker switch on the left side panel shown below Fibre In Interlock Output ON OFF Switch Turn on the BCU using the toggle switch on the front panel refer to the Borealis Hardware Guide Borealis ANDOR Emission Laser Emission Status LEDs Output ON OFF Toggle Switc
37. nual as well as the computer controlled operation via sofwate such as uManager and Metamorph A LCD dimmer button is adjacent to the screen that allows the brightness of the LSD screen to be adjusted if necessary For future information please refer to Section 4 5 1 2 4 2 MANUAL SELECTOR MODULE The Manual Dichroic Selector Module enables the user to easily select the required dichroic or bypass The dichroics installed in each position are indicated on a label the Module 2 4 3 Disk Cassette ACCESS DOOR The Disk Cassette Access Door enables access to the Disk Cassette so that the Disk Cassette may be exchanged For further information on exchanging cassettes please refer to Section 4 7 1 The Disk Cassette Access Door is held closed magnetically Microswitches indicate if the door is open or closed and are linked to the laser safety interlock system Section 2 9 Therefore if the door is opened lasers are switched off 2 5 POWER SUPPLY The Diskovery is supplied with a 12V DC 5 5A certified power supply Do not use any other power supply For further information please refer to Section 7 4 PSU Specifications Summary 100 240 V AC 50 60 Hz 12 V DC 5 5 A Version 1 1 rev 04 June 2015 23 ANDOR company PRODUCT OVERVIEW 2 6 DISKOVERY SYSTEM INTEGRATION 2 6 1 OvERMEW The integration of the Diskovery system involves the connection of the laser engine such as the ILE to the Diskovery unit via multi mo
38. or skin can be exposed without consequential injury immediately or after a long time and are related to the wavelength of the laser radiation the pulse duration or exposure duration the tissue at risk and for visible and near infra red laser radiation in the range 400 nm to 1 400 nm the size of the retinal image Maximum permissible exposure levels are in the existing state of knowledge specified in Annex A of IEC 60825 1 Reflection from a surface that can be considered a beam including reflections from mirrored surfaces Total Internal Reflection Fluorescence Microscopy le ee Version 1 1 rev 04 June 2015 46 ANDOR company OTHER INFORMATION APPENDIX C OTHER INFORMATION TERMS AND CONDITIONS OF SALE AND WARRANTY INFORMATION The terms and conditions of sale including warranty conditions will have been made available during the ordering process The current version may be viewed at http www andor com pdfs literature Andor Standard Warranty pdf Waste ELECTRONIC AND ELECTRICAL EQUIPMENT REGULATIONS 2006 WEEE The company s statement on the disposal of WEEE can be found in the Terms and Conditions 9 o Version 1 1 rev 04 June 2015 47
39. pped In some units the user can also change the excitation filter if the excitation filter wheel is present Any combination of configuration settings can be saved and recalled at a later time Field of illumination size The user can select from one of four illumination sizes shown in the left hand side of the GUI The labelling can be customized to a particular application In general the top two sizes are for confocal or traditional widefield while the bottom two are smaller fields intended for high intensity illumination used in super resolution applications Confocal 1 Pinhole size select one of two available pinhole sizes 3 on some obsolete disks as shown in the figures above Change will be made in approximately 6 seconds 2 Exchange to exchange or remove confocal pinhole disk cassette the lasers should first be turned off The exchange button can then be pressed to move the disk cassette to the exchange position The disk cassette is removed through the access door after unlocking using a 3 mm driver see Section 4 7 1 3 Disk start stop This allows the disk rotation to be stopped This function is used mainly by the installer to check that the pinholes are well focused on the camera TIRF The TIRF functionality cannot be controlled through the Diskovery Platform LCD touchpad user interface screen User s can only enable or disable the TIRF capability through the touchpad The TIRF module can only be controlled through
40. put via imaging optics and an emission filter wheel A second camera for the vertical output can be added that interfaces with imaging optics and a second emission filter wheel A camera selector or an emission light splitter is provided that allows selection of either camera or both via a manual slide in mirror or a dichroic mirror respectively Camera mounted on vertical output Emission Filter Wheel for horizontal output Emission Filter Wheel for vertical output Camera mounted on horizontal output Disk Cassette Access Door Left Side Panel Support Sled Manual Dichroic LCD Selector module Touchscreen Figure 10 Overview of the main hardware features of a typical Diskovery configuration 9 9 Version 1 1 rev 04 June 2015 21 ANDOR company PRODUCT OVERVIEW 2 3 INTERFACES AND CONTROLS LEFT SIDE PANEL The connections and interfaces on the left side panel of the Diskovery are outlined in the following section Fibre Input Power Fibre In Input Interlock ep o Interlock Output ON OFF Switch USB Figure 11 Left Side Panel of the Diskovery 2 3 1 POWER INPUT A 2 5 mm input connector on the left side panel is used to provide DC power from the power supply unit to the Diskovery The Diskovery requires a Direct Current DC power supply This is provided by the supplied external PSU For further information please refer to Section
41. re Continuous Wave output Pulse Repetition N A All recommended laser sources for the Borealis Product are E Continuous Wave output Irregular Pulse N A All recommended laser sources for the Borealis Product are Pattern Continuous Wave output Version 1 1 rev 04 June 2015 12 ANDOR company DESCRIPTION OF EMITTED RADIATION FROM THE INTEGRATED LASER SOURCE This is dependent on the laser source integrated with the Diskovery system As there are multiple options available from Andor or Diskovery may be integrated with a customer s existing laser source please refer to the Laser Source s Explanatory Label and User Documentation for a description of its Emitted Radiation RECOMMENDED RESPONSIBILITIES OF A LASER SAFETY OFFICER These include but are not restricted to the following however national guidelines should also be referred to jP Ensuring all personnel requiring access to the product are fully trained in both using the product and the general use of Class 3B and Class 4 lasers see below Ensure users are familiar with the hazardous properties of lasers namely that laser safety hazards differ from those of normal light radiation sources as they are high intensity highly collimated beams of electromagnetic radiation Ensuring the equipment is used in a controlled area by trained end users in accordance with national guidelines Ensuring end users are familiar with the operation of the laser s key switch control interloc
42. s laser viewing cards for this purpose e g Thorlabs NIR Detector Card VCR5 for 700 1400 nm must be used along with the appropriate laser safety goggles Additional precautions may need to be implemented as the necessary precautions will be specific to each system installation configuration and typical mode of use The responsible Laser Safety Officer must assess and implement the necessary precautions to avoid possible exposure to hazardous radiation during use 9 9 Version 1 1 rev 04 June 2015 13 ANDOR company EYE PROTECTION Eye Protection is not required for the safe use of the device as the only radiation observable without intentional misuse is directionally stable diffuse and highly divergent from the designated aperture which is static and labelled If eye protection is deemed desirable by the local Laser Safety Officer Andor recommends the following products e 360 nm 510 nm Kentek KXP 4001 Spectacles 510 670 nm Kentek 6702 Spectacles 670 nm 800 nm Kentek KMZ 215C Spectacles VVORKING WITH OPTICAL FIBRES 1 Only service personnel authorized by Spectral or Andor should remove or inspect fibres 2 laser radiation passing through fibres is potentially hazardous so great care should be taken to avoid exposure to this radiation 3 The fibre can be easily damaged by bending or general mishandling Ensure that the minimum curvature is never exceeded when handling Recommen
43. supply cord is required ensure replacement is of same type and rating 16 While running an experiment try to keep room temperature as stable as possible 17 This equipment has not been designed and manufactured for the medical diagnosis of patients 18 Performance of the system may be adversely affected by rapidly changing environmental conditions or operation outside of the operating conditions specified in SECTION 7 TECHNICAL SPECIFICATIONS 19 Electromagnetic Compatibility This is a Class A product In a domestic environment this product may cause electromagnetic interference in which case the user may be required to take adequate measures 6 6 Version 1 1 rev 04 June 2015 6 ANDOR company 20 This product has been designed and tested to perform successfully in a normal basic electromagnetic environment e g a typical life science test laboratory as per the EU EMC Directive It is not designed to operate in a harsh electromagnetic environment e g close to the following equipment EMI RFI generators electrostatic field generators electromagnetic or radioactive devices plasma sources arc welders x ray instruments intense pulsed sources or other similar sources of high energy fields whose emissions are not within the normal range expected under the EU EMC Directive 21 Your product is a precision scientific instrument containing fragile components Always handle it with care 22 There are no user servic
44. the following information when connecting customer support Owner Information Institution Name Vendor Information mf fmf Equipment Information Model Serial No Check List Cable Connections Emission LEDs Mains Power MEE What is the nature of the problem Any other observations S Version 1 1 rev 04 June 2015 ANDOR company TECHNICAL SPECIFICATIONS SECTION 7 TECHNICAL SPECIFICATIONS 7 1 DISKOVERY GENERAL SPECIFICATIONS Input wavelength range 400 785 nm Input power 2W maximum from combined wavelengths Output As classified under IEC 60825 1 or the regional equivalent 500 mW max Class 3B typical May be classed Class 4 with lt 1W max with some laser combinations refer to Laser Safety and Classification sections in the preface of this manual Excitation Uniformity 10 rms 15 rms at 405 nm Maximum frame rate fps 1000 1 ms exposure 2 BELLOWS ADAPTER Standard Length range mm 60 12 Compact Bellows ENVIRONMENTAL SPECIFICATIONS lt 70 non condensing Rated Pollution Pollution Degree 2 Normally only non conductive pollution occurs Occasionally however a temporary conductivity caused by condensation must be expected Ventilation Requirements Do not cover during operation 4 POWER SPECIFICATIONS Power Supply Output Connector 2 5 mm coaxial barrel power plug centre posi
45. tive inner outer diameter 2 5 5 5 mm Power Consumption Diskovery External Power Supply 32 W 40 W Typ Max Diskovery Only 21 W 24 W Typ Max Overvoltage Category CAT Il An overvoltage category of CAT Il means that the equipment is designed to cope with transient voltages above the rated supply that would be experienced by any product connected to a mains socket in a building Version 1 1 rev 04 June 2015 44 ANDOR company MECHANICAL DRAWINGS APPENDIX A MECHANICAL DRAWINGS All Dimensions mm inches Diskovery Platform Standard Bellows Coupling Adapter Optional Compact Bellows Coupling Adapter Version 1 1 rev 04 June 2015 45 GLOSSARY ANDOR company APPENDIX B GLOSSARY AC AC DC ADC AEL BCU CSU CLSM DC Diffuse Reflection EMC ESD EU FC APC lO ILE Intrabeam Viewing LED LMM MM NOHD MPE Specular Reflection TIRF Alternating Current Mains An electronic device that converts AC electricity usually mains into a DC voltage usually a safe low voltage Analogue to Digital Converter Converts an analogue voltage to a digital signal Accessible Emission Limit The maximum accessible emission permitted within a particular class Wherever the text refers to emission level not exceeding the AEL or similar wording it is implicit that the accessible emission is determined following the measurement criteria specified in Clause 9 of IEC
46. tte motorized stage lock Figures 30a and 30b p Figure 30 Accessing stage low screw a and Using a 3 mm hex driver to unlock the stage b 5 Turn the Allen driver counter clockwise until the disk cassette releases and slides out towards to the access door slightly Version 1 1 rev 04 June 2015 38 ANDOR company OPERATION 6 Grab the disk cassette s two rounded corners facing outwards towards the door and pull the cassette straight out of the Diskovery platform 7 Close the confocal pinhole disk cassette access door 8 Store the un mounted disk cassette in a dust free environment or the provided disk cassette box Figure 31 E E 3 P Figure 31 Diskovery cassette storage box 4 2 EXCHANGING A DISK CASSETTE 1 To exchange a confocal pinhole disk cassette into the Diskovery Platform perform the steps outlined in Section 4 7 1 to remove the existing Disk Cassette Installation of a different Disk Cassette is the opposite of the removal procedure 2 Hold the disk cassette to be loaded into the Diskovery carefully by the two rounded corners facing outwards and press the cassette straight into position Usea3 mm hex driver to lock the Disk Cassette to the stage see figure 30 4 Close the Disk Cassette Access Door 9 9 9 Version 1 1 rev 04 June 2015
47. very LCD touchpad user interface on the front panel of the instrument 2 Using the Diskovery Control Utility supplied with the instrument This computer application is required to operate the TIRF module 4 5 1 LCD ToucHPAD USER INTERFACE The graphical user interface GUI of the Control Utility application was designed to mimic the layout of the touchpad user interface screen The figures included in this section apply to both the touchpad screen and the computer GUI except where noted Figures 16 and 17 show the main control panel for versions with and without the optional TIRF module respectively Figure 16 shows the GUI as it appears in the computer Control Utility application On the touchpad LCD screen the text outside the green box is not present Also note that the button labelling can be customized by the installer so the screen may not be identical to that shown below CONFOCAL T 1 TO ke Le 1 Figure 17 Main Screen of the Diskovery Control Utility without TIRF Version 1 1 rev 04 June 2015 31 ANDOR company OPERATION The main control panel shows all of the available non TIRF functions The user can select the square field of illumination size the confocal disk pinhole size and the microscope objective magnification The user can also insert and remove the disk from the optical path Disk Out for widefield or TIRF The disk can also be sto
48. w spectral ca ANDOR an Oxford Instruments company Andor Technology An Oxford Instruments Company Millennium Way opringvale Business Park Belfast BT12 7AL Northern Ireland Web www andor com Version 1 1 rev 04 June 2015 15 ANDOR company INTRODUCTION 1 1 TECHNICAL SUPPORT If you have any questions regarding the use of this equipment please contact the representative from whom your system was purchased or Europe USA Andor Technology Andor Technology Millennium Way 425 Sullivan Avenue opringvale Business Park Suite 3 Belfast South Windsor BT12 7AL CT 06074 Northern Ireland USA Tel 44 0 28 9023 7126 Tel 1 860 290 9211 Fax 44 0 28 9031 0792 Fax 1 860 290 9566 www andor com contact_us support_request www andor com contact_us support_request Asia Pacific China Andor Technology Japan Andor Technology IS Building Room 1213 Building B 3 32 42 Higashi Shinagawa Luo Ke Time Square Shinagawa ku No 103 Huizhongli Tokyo 140 0002 Chaoyang District Japan Beijing 100101 PR Tel 81 3 6732 8968 China Fax 81 3 6732 8939 Tel 86 0 10 51294977 Fax 86 0 10 6445 5401 www andor com contact us support request www andor com contact us support request The latest contact details for your local representative can be found on our website Version 1 1 rev 04 June 2015 16 ANDOR company INTRODUCTION 1 2 DISCLAIMER THE INFORMATION CONTAINED HEREIN IS PROVIDED
49. y two people 6 Ensure fibre cables are not bent tightly as this may damage the internal optical fibre For transport or storage fibre cables should be coiled with a diameter of 300 mm or greater 7 Locate the laser source BCU and Diskovery within 2 m of each other so that the interconnecting fibres are not stretched bent or constrained 8 This product will be used with lasers 9 Technical Document IEC TR 60825 14 recommends the presence of a Laser Safety Officer LSO however national guidelines should be referred to 10 Do not attempt to bypass any safety interlocks They are provided to comply with the safety requirements of various regulatory agencies and must be employed to protect the operator 11 Protective earth is an integral part of the protection against electric shock in this product and is provided via the earth pin of the external power supply Ensure that this is plugged into the building earth system via the mains socket Do not tamper with any of the earthing measures 12 Only the correctly specified mains supply should be used 13 Only the AC DC external power supply provided with the product should be used 14 Only the power supply cord provided with the product should be used Should this not be correct for your geographical area contact your local Andor representative 15 Make sure the power supply cord is located so that it will not be subject to damage If replacement of the detachable power
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