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Agilent 1290 Infinity Binary Pump User Manual

1. 10 Click Install to begin installation Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 11 Files load when finished the BootP Settings screen appears BootP Settings a BootP Tab File Create Tab File Edit BootP Addresses Logging BootP Log File E Default Settings Subnet mask GO 0 00 Gateway GO 0 0 0 Cancel Help Figure 51 BootP Settings screen 12 In the Default Settings part of the screen if known you can enter the subnet mask and gateway Defaults can be used The default subnet mask is 255 255 255 0 The default gateway is 192 168 254 11 13 On the BootP Settings screen click OK The Agilent BootP Service Setup screen indicates completion 14 Click Finish to exit the Agilent BootP Service Setup screen 15 Remove the DVD from the drive This completes installation 16 Start BootP Service in the Windows services On the Windows desktop click right on Computer icon select Manage gt Services and Applications gt Services Select the Agilent BootP Service and click Start Agilent 1290 Infinity Binary Pump User Manual 271 13 LAN Configuration 278 Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address select the Do you want to log BootP requests check box 1 Open BootP Settings from Start gt All Programs gt Agilent BootP Service gt EditBootPSett
2. Accessory Kit p n G4220 68705 5042 9974 8710 0510 8710 1924 5023 0240 8710 2392 9301 0411 9301 1337 5067 4657 5067 4658 0100 1710 G4220 67000 8710 1534 5065 9978 5181 1519 01018 22707 01018 23702 5042 9972 Parts and Materials for Maintenance 10 Description Accessory kit Tubing Flex 1 5 m Wrench open 1 4 5 16 inch Wrench open 14 mm Hex driver 4 slitted Hex key 4mm15 cm long T handle Syringe Plastic Syringe adapter Capillary ST 0 17 mm x 300 mm Pump to Autosampler SST Capillary 450 x 0 17 mm Pump to Thermostatted Autosampler Mounting Tool for Tubing Connections Waste tubes Wrench 4 mm both ends open end Tubing 1 mm i d 3 mm o d silicone 5 m for seal wash option CAN cable Agilent module to module 1 m PTFE frits pack of 5 Insert tool Tubing grommet Agilent 1290 Infinity Binary Pump User Manual 225 10 Parts and Materials for Maintenance Tools Item p n Description 5067 4699 1290 Infinity pump service kit 1 G4220 20012 Torque wrench 2 25 Nm 2 G4220 20013 4 mm hex bit 3 G4220 20014 2 5 mm Hex Bit 4 G4220 20015 Adapter in square to hex 5023 0285 Replacement kit for 1290 Infinity pump head alignment tool piston handle G4220 44000 Protective Foam G6500 88888 Hexalobular Key Set 8710 0803 Wrench 9 16 inch 7 16 inch The 1290 Infinity pump service kit 5067 4699 includes pump head alignment tool and items 1 4 Figure 35 Pump head alignment tool 22
3. e Minimize exposure to light and air oxygen e Choose a bottle size which fits to your application and usage e Acids accelerate polymerization If possible avoid such additives or refresh solvents more frequently e Pure acetonitrile polymerizes faster If your application allows add about 5 water and adjust gradient compositions e Do not leave acetonitrile in unused systems to avoid aging If not in use flush all solvent lines with a mixture of water and 10 isopropanol In case of blocked valves flush the system with hot water Knock at valves flush them see Releasing a Stuck Inlet Valve on page 153 or ultrasonicate them e g in methanol Handling of Acids Acids can corrode stainless steel and other materials in the flow path of LC systems For stainless steel the minimum pH is 2 3 for corrosive acids and pH 1 for non corrosive acids Please note that for non volatile acids like phosphoric acid or perchloric acid concentrations increase after evaporation of water This means that originally diluted acids can damage parts over time e g because of liquid which has left the solvent path through micro leaks Such systems should be flushed regularly with pure water and may require shorter maintenance cycles Using a seal wash option should be considered for protecting pump heads Agilent 1290 Infinity Binary Pump User Manual 73 4 Using the Pump Normal Phase Applications CAUTION Current valves used wi
4. Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides Agilent 1290 Infinity Binary Pump User Manual 131 9 Maintenance CAUTION Safety standards for external equipment gt If you connect externa
5. correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s documentation Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact y
6. detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Table 12 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out D
7. gt Avoid loops gt Tubes must not sag gt Do not bend tubes gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module Agilent 1290 Infinity Binary Pump User Manual 37 3 Installing the Module sa i tallatinn Infn Inst lle iON INnTori Warning Attention Warnung Attenzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant tout d placement Entfernen Sie das Netzkabel bevor Sie das Gerat bewegen Scollegare il cavo di alimentazione prima di spostare lo strumento Desconectar el aparato de la red antes de moverlo L 2J I Figure 8 Warning label illustration for correct waste tubing 38 Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 Removing the Transport Foam Removing the Transport Foam 1 Open the front cover of the module 2 Carfully remove the protective foam 3 Close the front cover Agilent 1290 Infinity Binary Pump User Manual 39 3 Installing the Module Installing the Pump Installing the Pump Parts required Description 1 Pump 1 Power cord 1 Agilent Control Software and or Instant Pilot G4208 Preparations Locate bench space Provide power connections Unpack the pump 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the module
8. gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Incorrect line voltage at the module Shock hazard or damage of your instrument can result if the devices are connected to line voltage higher than specified gt Connect your module to the specified line voltage CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 20 Agilent 1290 Infinity Binary Pump User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a po
9. insufficient power of drive encoder LED 112 interfaces 249 internet 298 J Jet Weaver 149 remove capillary connections 150 L Lab Advisor Software tests 120 LAN automatic configuration with Bootp 274 Bootp amp Store 267 Bootp 266 Agilent 1290 Infinity Binary Pump User Manual cable 239 configuration switche 265 configuration 261 initialization mode selection 266 link configuration selection 273 manual configuration with telnet 285 manual configuration 284 PC and User Interface Software Setup 290 TCP IP parameter configuration 264 using default 268 using stored 268 what you have to do first 262 eak sensor open 98 eak sensor short 97 eak 100 ine frequency 23 ine voltage 23 ink configuration selection 273 ost CAN partner 97 M MAC address determine 279 MAC address 262 maintenance feedback 260 introduction 129 replacing firmware 196 manual configuration of LAN 284 message cover violation 101 ignition without cover 100 100 remote timeout 96 minimum flow rate 51 non operating altitude 23 non operating temperature 23 normal phase 74 seals 74 0 operating Altitude 23 operating temperature 23 optimization achieving higher resolution 81 stack configuration 30 outlet valve 144 overcurrent of pump drive 109 overcurrent of solvent selection valve SSV 109 P packaging damaged 28 parts damaged 29 missing 29 system tool kit 228 PC and User Interface Software Setup 290 perform
10. G4220 60028 64220 20020 Spanner double open ended 12X14 mm Chrome Torque wrench 2 25 Nm Bit Torx 10x25 mm Description Outlet valve primary pump head Internal gold seal for Outlet Valve 1 Close the shut off valves to avoid solvent leaks 2 Lift up the capillary and remove it from the primary pump head 144 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Outlet Valve 3 A gold seal between outlet valve and heat exchanger capillary is used for a tight connection The seal can be replaced separately as needed Hex screw es iS N Gold seal lt a R Aa A Heat exchanger Se A 4 Unscrew the outlet valve with a 14 mm wrench 5 Insert the outlet valve into the pump head Using a torque wrench set 10 Nm and close the outlet valve 6 Insert the heat exchanger capillary into the outlet of the outlet valve Using a torque wrench with a 2 5 mm hex bit set 3 Nm and close the hex screw at the top of the outlet Agilent 1290 Infinity Binary Pump User Manual 145 Maintenance Replacing the Outlet Valve 7 146 Open the shut off valves and purge the system to remove air Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Solvent Selection Valve SSV Replacing the Solvent Selection Valve SSV When In case of problems with the solve
11. Py Pump heads Purge valve Pressure sensor The pump is shipped with tubing and capillary connections installed between solvent selection valve degassing unit pump heads pressure sensor purge valve and Jet Weaver This section describes the installation of additional flow connections Agilent 1290 Infinity Binary Pump User Manual 43 3 Installing the Module Parts required p n Description 1 Other modules 1 G4220 68705 Accessory kit 1 G5067 1531 Solvent Cabinet Kit Preparations Pump is installed in the LC system WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Remove the front cover by pressing the snap fasteners on both sides Figure 11 Removing the Front Cover 44 Agilent 1290 Infinity Binary Pump User Manual A2 Al Installing the Module 3 Flow connections to the pump 2 Place the solvent cabinet on top of the module stack that includes the 1290 Infinity Binary Pump
12. 24 This Binary pump has been introduced together with the Agilent 1290 Infinity Liquid Chromatograph 10 Agilent 1290 Infinity Binary Pump User Manual Introduction 1 Overview of the Binary Pump The Agilent 1290 Infinity Binary Pump comprises two identical pumps integrated into one housing Binary gradients are created by high pressure mixing A degassing unit is included for applications that require best flow stability especially at low flow rates for maximum detector sensitivity The flow path of the pump has been optimized for minimal delay of gradients Typical applications are high throughput methods with fast gradients on high resolution 2 1 mm columns The pump is capable of delivering flow in the range of 0 05 5 mL min against up to 1200 bar A solvent selection valve allows forming binary mixtures isocratic or gradient from one of two solvents per channel Active seal wash optional is available for use with concentrated buffer solutions Agilent 1290 Infinity Binary Pump User Manual 11 1 Introduction Pump Principle 12 The Binary Pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by two pump assemblies which can generate pressure up to 1200 bar Each channel comprises a pump assembly including independent pump drive for each piston pump
13. 3 Put the four bottles into the solvent cabinet and screw a bottle head assembly onto each bottle 4 Install the shutoff valve panel at the top left corner of the instrument 5 Connect the solvent tubes from the bottle head assemblies to the inlet connectors Al A2 B1 and B2 of the solvent selection valves Use the brown bottle for the aqueous solvent usually channel A1 ee Ca ADO AN AA RET SS SE I was SANS U S Agilent 1290 Infinity Binary Pump User Manual 45 3 46 Installing the Module 6 Label the tubes accordingly using the supplied stickers and fix the tubes in the clips of solvent cabinet and 1290 Infinity Binary Pump 7 Connect the outlet of the Jet Weaver to the autosampler 8 Connect Waste tubes G4220 67000 to the purge valve outlets at ports 5 and 6 9 Connect the corrugated waste tube to the outlet of the leak panel 10 Route the corrugated waste tube to a waste container 11 Route drain tubes coming from modules on top of the pump through the pump 12 Purge your system prior to the first use see Priming the Pump on page 64 Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 Installation of Seal Wash Option Installation of Seal Wash Option To wash solvent reservoir To waste container Figure 12 Binary Pump with Seal Wash Function The 1290 Infinity Binary Pump is optio
14. 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 240 Agilent 1290 Infinity Binary Pump User Manual Identifying Cables 11 Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module Agilent 1290 Infinity Binary Pump User Manual 241 11 Identifying Cables Agilent 1200 Module to Printer 242 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 12 Hardware Information Firmware Description 244 Electrical Connections 247 Rear View of the Module 248 Interfaces 249 Overview Interfaces 252 Setting the 8 bit Configuration Switch 256 Special Settings 258 Instrument Layout 259 Early Maintenance Feedback 260 This chapter describes the pump in more detail on hardware and electronics ait Agilent Technologies 243 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system M
15. Cable Overview 230 Analog cables 232 Remote Cables 234 BCD Cables 237 CAN LAN Cable 239 RS 232 Cable Kit 240 Agilent 1200 Module to Printer 241 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ott Agilent Technologies 229 11 Identifying Cables Cable Overview 230 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 234 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose Agilent 1290 Infinity Binary Pump User Manual CAN cables p n 5181 1516 5181 1519 LAN cables p n 5023 0203 5023 0202 RS 232 cables p n G1530 60600 RS232 61601 5181 1561 Identifying Cables 11 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m
16. Configuration Switch LAN Configuration 13 Configuration Switch The configuration switch can be accessed at the rear of the module D SS amp YcCZ 4c JC C c a gt S aorta REMOTE my O Je sfc 65 gt CSS SoS SS CZ gt C AS 2332 use 2 oman eee I 9c gt ae Cl Gee gt gt ia m m Q Qa Oo 0 m Qa Qa oO LLL Scapa D SSZ nnoaaagaacaaa Figure 44 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above NOTE To perform any LAN configuration SW1 and SW2 must be set to OFF Table 17 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Initialization mode selection on page 266 Link Configuration speed and duplex mode determined by auto negotiation for details see Link configuration selection on page 273 Agilent 1290 Infinity Binary Pump User Manual 265 13 LAN Configuration Initialization mode selection Table 18 The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default ON OFF OFF DHCP 1 Requires firmware B 06 40 or above Modules without LAN on board see G1369C LAN Interface Card 266 Bootp When the initializa
17. Failed Probable cause Suggested actions 1 Damaged blank nut poorly shaped from Before investigating any other possible sources over tightening of failure make sure that the blank nut you are using is in a good condition and properly tightened 2 Pump leakages Perform the Pump Head Leak test 3 Loose or leaky fittings Tighten the fittings or replace capillaries 4 Autosampler leakages Perform the Autosampler Leak test 5 Themostatted Column Compartment valve Replace the TCC valve rotor seal leakages Notice the difference between error in the test and a failed result An error is caused by an abnormal termination during the operation of the test whereas a failed result indicates that the test result were not within the specified limits Agilent 1290 Infinity Binary Pump User Manual 123 8 Test Functions and Calibrations Pump Head Leak Test The test determines the leakage of the individual pump heads by blocking each pump head separately and pressurizing to a specified level The flow is delivered by the secondary piston and thereby the parts between the outlet ball valve and the purge valve are tested for leak tightness The test allows for setting the pressure at which the test is performed The leak rate of high pressure parts is not always a linear function and therefore it is recommended to perform the test at a pressure that corresponds to the normal operating pressure of the system When Excessive pressure ripple or suspec
18. Figure 46 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp Agilent 1290 Infinity Binary Pump User Manual 267 13 LAN Configuration 268 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 47 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 19 on page 268 Default Active Parameter Parameter Figure 48 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Table 19 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 Since the default IP address is a so called local addre
19. Infinity Binary Pump User Manual 281 13 LAN Configuration Changing the IP Address of an Instrument Using the Agilent BootP Service Agilent BootP Service starts automatically when your PC reboots To change Agilent BootP Service settings you must stop the service make the changes and then restart the service Stopping the Agilent BootP Service 1 From the Windows control panel select Administrative Tools gt Services The Services screen appears RE Be aton yew teb s B iDRBI r gt r gt s n Select an tem to view ts description Boverrdeted Transat Coor P pnp 2S Figure 54 Windows Services screen 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools screen 282 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 Editing the IP address and other parameters in EditBootPSettings 1 Select Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 When the BootP Settings screen is first opened it shows the default settings from installation 3 Press Edit BootP Addresses to edit the Tab File x Host Name IP Address Comment Gateway 006000111599 Agier LC1 1011 10 Agiert LET ngtt 285 255 2550 0000 005000222883 Ager C2 101 1 1 102 Agiert LC2 left 252552550 0000 Figure 55 Edit BootP Adresses screen 4 In the Edit BootP Addresses screen press Add to create a new
20. Parts for details Inserting the backup seal wrongly may limit the life time of the pump head gt Please note the correct orientation of the backup seal CAUTION Limited life time of the pump head INETI Damage of the pump piston The pump piston is very sensitive to shearing forces from the side gt Use the alignment piston of the pump head alignment tool for the alignment procedure described below 176 Agilent 1290 Infinity Binary Pump User Manual CAUTION Maintenance 9 Wrong orientation of pins on support ring Assembling the pump head without paying attention to the correct orientation of the pins on the support ring can lead to leaks or damage of the piston and pump head gt Observe pins on the support ring which help assembling the parts of the pump head in the correct orientation CAUTION Damage of the pump head assembly When installing the pump head assembly the pump drives need to be in maintenance position where they are retracted Using the pump drive in default position will damage the pump head assembly gt Bring the pump drive to the maintenance position CAUTION This procedure describes how to assemble the secondary pump head using the pump head alignment tool Assembling the primary pump head can be done accordingly The secondary pump head has the heat exchanger capillary which must fit into the openings of the alignment tool whereas the primary pump head does not have a hea
21. Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1290 Infinity Binary Pump User Manual 235 11 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 236 Agilent 1290 Infinity Binary Pump User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White gr
22. Pressure Limits Max 1200 bar 17400 psi for flow rates up to 2 mL min For flow rates between 2 mL min and 5 mL min the maximum pressure ramps down to 800 bar 11600 psi Min any value between 0 and the upper pressure limit setting Timetable Advanced External Contacts Sets the maximum and minimum pressure limits for the pump Max is the maximum pressure limit at which the pump will switch itself off protecting the analytical system against over pressure Minis the minimum limit at which the pump will switch itself off for example if any solvent reservoir is empty this prevents system damage by pumping air See Timetable Settings on page 63 See Advanced Settings on page 62 The External Contacts section enables you to set up the switching of the external contacts The External Contacts section is present only when a BCD external contacts board is installed Agilent 1290 Infinity Binary Pump User Manual 61 4 Using the Pump Advanced Settings The Binary Pump advanced method setup parameters are in three sections e Minimum Stroke e Compressibility e Maximum Flow Gradient Table6 Advanced method parameters Parameter Limits Description Minimum Stroke Compressibility Maximum Flow Gradient 20 100 pL 1 000 1000 000 mL min mi nin steps of 0 001 mL min min Default value 100 000 mL min min The Stroke Volume is used for optimizing between per
23. Pump User Manual Error Information 7 Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause Suggested actions 1 Leak detected in another module with a Fix the leak in the external instrument before CAN connection to the system restarting the module 2 Leak detected in an external instrument Fix the leak in the external instrument before with a remote connection to the system restarting the module 3 Shut down in an external instrument with a Check external instruments for a shut down remote connection to the system condition Agilent 1290 Infinity Binary Pump User Manual 95 7 96 Error Information Remote Timeout Error ID 0070 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up
24. REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces The module includes a DC Out 24 VDC power line that is intended to be used with certain modules that operate as CAN slaves for example external valves The line has a limited output of 0 5 A 1 7 A as of August 2011 and is self resetting Agilent 1290 Infinity Binary Pump User Manual 255 12 Hardware Information Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e Default is ALL switches DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode For normal operation use the default best settings SX Agilent Tecdnsiogies VUeEUUUYUYUY 256 Figure 41 Location of Configuration Switch example shows a G4212A DAD Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Table 14 8 bit Conf
25. The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal CAN Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product 9 The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up gt WARNING ahaa alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 294 Agilent 1290 Infinity Binary Pump User Manual Appendix 14 General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the pr
26. What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small molecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and that can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be offset by reducing the temperature of the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve shows that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns Agilent 1290 Infinity Binary Pump User Manual Optimizing Performance 5 In isocratic separations increasing the retention factor k results in better resoluti
27. alignment tool Lubricate the alignment piston with isopropanol or another suitable solvent before insertion Alignment piston 5 CAUTION Damage to the pump head gt Using the alignment tool is mandatory gt Not using will break the pump head 6 Insert the pump head to the pump head alignment tool which is included to the 1290 Infinity Service Kit p n 5067 4699 There are openings for the seal wash support ring and heat exchanger of the secondary pump head Observe the correct orientation of all parts Pump head alignment tool rear al h Ds Pump head alignment tool front 180 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 7 Close the tool by closing the 3 screws at the connection ring Heat exchanger capillary CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 8 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the central alignment screw entra alignment screw Agilent 1290 Infinity Binary Pump User Manual 181 9 Maintenance CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wre
28. available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1290 Infinity Binary Pump User Manual Appendix 14 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical an
29. column 6 Observe the pressure fluctuations The system is air free as soon as the pressure is stable 7 Change solvents and column according to the analytical conditions and purge the system to change solvents Agilent 1290 Infinity Binary Pump User Manual 65 4 Using the Pump Solvent Information 66 Introduction Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 52 Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Agilent 1290 Infinity Binary Pump User Manual Materials in Flow Path Following materials a Using the Pump 4 re used in the flow path of this module Part Materials Degasser chamber Ultra clean tubings Microfluidic structures2 SSV Passive inlet valve Outlet valve Pump head Pistons Piston wash seals Pressure sensor Automatic purge valve TFE PDD copolymer PFA internal tubings PEEK inlets FEP tubings ETFE fittings PFA tubings PEEK fittings SST PEEK FEP PFA Alz03 based ceramic ruby sapphire SST SST gold ruby ZrO9 based ceramic ta
30. describes the tests for the module 9 Maintenance This chapter describes the maintenance of the Agilent 1290 Infinity Binary Pump 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the pump in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the detector to the Agilent ChemStation PC 14 Appendix This chapter provides addition information on safety legal and web Agilent 1290 Infinity Binary Pump User Manual Contents Contents 1 Introduction 9 Features 10 Overview of the Binary Pump 11 Pump Principle 12 System Overview 15 2 Site Requirements and Specifications 19 Site Requirements 20 Physical Specifications 23 Specifications 24 3 Installing the Module 27 Unpacking the Module 28 Optimizing the Stack Configuration 30 Installation Information on Leak and Waste Handling 35 Removing the Transport Foam 39 Installing the Pump 40 Flow connections to the pump 43 Installation of Seal Wash Option 47 4 Usingthe Pump 49 Leak and Waste Handling 50 Preparing the Binary Pump 51 Algae Growth in HPLC Systems 52 Setting up the Pump with the Instrument Control Interface 54 Priming the Pump 64 Solvent Information 66 Normal Ph
31. detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual 99 7 Error Information Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Open Cover Error ID 0205 The top foam has been removed Probable cause Suggested actions 1 Foam not activating the sensor Please contact your Agilent service representative 2 Defective sensor or main board Please contact your Agilent service representative 100 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Cover Violation Error ID 7461 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is remo
32. fuses can result in fire hazard gt For continued protection against fire hazard replace line fuses only with the same type and ratings gt Only use the fuses specified for this instrument The use of other fuses or materials is prohibited 194 There are more fuses inside the instrument If replacing the main power fuse does not resolve the issue please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual Maintenance Replacing the Main Power Fuses 1 Use a screwdriver for removing the main fuse carrier from the compartment next to the main power plug Remove the fuse from the carrier 2 Install the new fuse 10A to the carrier and insert the carrier to the fuse compartment BAB FFA Zo 06 250 KK MQ x TALEO X a Ws a AN Agilent 1290 Infinity Binary Pump User Manual 195 9 Maintenance Replacing Module Firmware When Tools required OR OR Parts required Preparations 196 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version De
33. head inlet valve outlet valve solvent heat exchanger and an outlet filter The two channels are fed into a low volume mixing groove in an automatic purge valve and a Jet Weaver mixer with 35or 100 ul volume can be added downstream for optimum mixing performance A system pressure sensor for monitoring the pump pressure is attached to the purge valve normally connected in the B channel of the pump before the mixing groove in order to minimize delay volumes Agilent 1290 Infinity Binary Pump User Manual Jet Weaver l Purge valve Pressure sensor High pressure filter assembly Piston Outlet valve Seal Inlet valve Degasser Solvent selection valve Solvent bottles Introduction 1 Pump Principle Pump head B Pump head A Figure 1 The hydraulic path Agilent 1290 Infinity Binary Pump User Manual 13 1 14 Introduction In the user interface the solvent in use for each channel can be selected in order to get optimum flow and composition accuracy Although selecting the right solvent is not required for low ripple especially for composition accuracy across pressure it is vital for the compensation algorithm to use the right solvent properties The density of the solvents is increased under the influence of pressure and a certain displaced solvent will expand again when the pressure is released for example across the column In order to achieve the correct volumetric flow while sa
34. high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens Agilent 1290 Infinity Binary Pump User Manual 69 4 70 Using the Pump High concentrations of inorganic acids like nitric acid sulfuric aci
35. how firmly it has been tightened firm tightening reduces the lifetime If fitting has been overtightened replace it 1 Install fittings and capillaries 2 Tighten fittings and capillaries Agilent 1290 Infinity Binary Pump User Manual 135 9 Maintenance Replacing the Shutoff Valve Panel Replacing the Shutoff Valve Panel When If a shutoff valve is damaged or the panel needs to be removed for other repair procedures Parts required p n Description 1 5067 4124 Shutoff valve 1 G4220 40004 Shutoff valve panel 2 G4220 60035 Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit Preparations In order to avoid leaks remove tubings from the solvent bottles 1 Unscrew tubing connections between shutoff valves 2 Ifa single valve shall be replaced it can be pulled to the solvent bottles and the solvent selection valve front for removing it from its mounting 136 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Shutoff Valve Panel 3 Remove the shutoff valve panel by pulling it downwards Al B1 has PF fp A B A O v B TTE 4 After replacing the panel or after completion of other maintenance re install the panel and all flow connections Agilent 1290 Infinity Binary Pump User Manual 137 9 Maintenance Replacing the Pressure Sensor When No or invalid pressure signal Tools required
36. is OFF switch stands out i E LI Power switch Qy j F F Serial number Figure 9 Front of Binary Pump 40 Agilent 1290 Infinity Binary Pump User Manual APG Remote AN Bus Configuration switch Power supply for externa CAN valves AN Compact Flash Power plug Installing the Module 3 Installing the Pump 3 Connect the power cable to the power connector at the back of the 1290 Infinity Binary Pump 4 Connect the required interface cables to the rear of the 1290 Infinity Binary Pump CDe CC a ote lt JC IEE Y pia 2L IC a a gt DC DC ote IT TOC P 9 Ce h Ei COMPACT FLASH AS 232 USB LAN ZE Bans aoc SA SX CDC zat CLOC gt gt 2 gt D ZZ CDE gt D 0 gt C IC C BY gZ ye Z c IC ME gt a 3 V gt le C oa ia 3C IC Gz 5 c 5 iS y C b Ta cr mn i c JC SS c c c E i E o gt C he gt is she DC DC gt gt Ja afi 5 c gt gt 3 c 5 gt gt C C gt amp gt C C p p C BD amp gt CSO aC c DB SC EE ZD TAN ZS 5 CES Ca O oD Co 33 azz Gmaaapbe 3 CBZ q GQpaS ZD ag Qpaaazz C So apas4 C cma A an a m T a a C U SSaB
37. is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide AlyOg are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Agilent 1290 Infinity Binary Pump User Manual 71 4 Using the Pump Solvent Handling Handling of Buffers The following recommendations should be observed when using buffer solutions 72 Buffers and aqueous solutions are possible sources of algae contamination for avoiding related problems please read Algae Growth in HPLC Systems on page 52 For buffer concentrations of 0 1 M or higher using the seal wash option is strongly recommended Filter buffer solutions to avoid increased wear or blockages that are caused by undissolved crystals Always use solvent inlet filters Avoid conditions where mixing of buffers and organic solvents may cause precipitation as this impairs the reproducibility of chromatographic experiments and may also reduce the system life time For example in reversed phase chromatography avoid buffers especially phosphate buffers with a concentration higher than 20 mmol L For phosphate buffers avoid compositions containing more than 65 acetonitrile or other
38. of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 15 Boot Resident Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 1 0 0 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 16 Forced Cold Start Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 0 0 0 1 258 Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages
39. of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1290 Infinity Binary Pump User Manual 259 12 Hardware Information Early Maintenance Feedback 260 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the
40. p n Description 8710 2412 Hex key 2 5 mm 15 cm long straight handle 8710 0510 Wrench open 1 4 5 16 inch Screwdriver Parts required p n Description 1 G4220 60001 Pressure sensor 1200 bar Preparations Turn off pump flow switch off pump This procedure describes how to replace the pressure sensor In case the cable to the sensor shall be replaced as well please contact your Agilent service representative 138 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Pressure Sensor 1 Remove capillary connections between the pressure 2 Remove the screws that fix the pressure sensor to the sensor and purge valve chassis 3 Carefully pull out the pressure sensor for about 2 cm 4 Connect the new pressure sensor to the pressure sensor Then unscrew the cable from the pressure sensor connector Agilent 1290 Infinity Binary Pump User Manual 139 9 Maintenance Replacing the Pressure Sensor 5 Fix the pressure sensor to the instrument chassis 6 Connect the capillaries from the valve to the pressure sensor connect port 3 to the pressure sensor inlet and port 2 to the outlet 140 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Overview of Torques for Pump Head Procedures Overview of Torques for Pump Head Procedures Fig
41. primary pump head Heat exchanger with connection capillary soe f 2 Remove both pump heads from the link plate by pushing the clips at the rear of the plate Link plate 162 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Disassembling the Primary Pump Head SVTETT Damage of pump piston The pump piston is made of Zr04 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 Remove the outlet valve at the top of the pump head and 2 For disassembling the pump head remove the 4 hex the inlet valve at the bottom of the pump head screws at the rear of the pump head Outlet valve Inlet valve Agilent 1290 Infinity Binary Pump User Manual 163 9 Maintenance 3 Remove the front part of the pump head including pump chamber housing with pump seal and seal holder If the seal wash option is installed also remove the support ring with wash seal and gasket Support ring with wash seal Seal wash gasket Seal holder including backup ring 4 Remove the front part of the pump head including pump chamber housing with pump seal and seal holder Support ring including backup ring Seal Pump chamber housing 5 Remove the piston from the piston housing by p
42. seal for Outlet Valve 213 10 Parts and Materials for Maintenance Primary Pump Head Without Seal Wash Binary Pump Figure 28 Primary pump head Binary Pump without seal wash 214 Agilent 1290 Infinity Binary Pump User Manual Item p n 1 0515 1218 3 0905 1719 4 G4220 60015 5 6 7 5067 5678 8 G4220 60022 9 G4220 60028 10 G4220 20028 11 G4220 20020 Agilent 1290 Infinity Binary Pump User Manual 10 Parts and Materials for Maintenance Description Screw M5 40 mm long Pump Chamber Housing order pump head PE Seal Support ring including backup ring Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic Inlet valve primary pump head Outlet valve primary pump head Headless screw for 1290 Infinity pump heads Internal gold seal for Outlet Valve 215 10 Parts and Materials for Maintenance Secondary Pump Head Parts Secondary Pump Head With Seal Wash Binary Pump 16 Figure 29 Secondary pump head Binary Pump with seal wash 216 Agilent 1290 Infinity Binary Pump User Manual Item 10 11 12 13 14 15 16 17 18 Agilent 1290 Infinity Binary Pump User Manual p n 0515 1218 G4220 20003 G4220 81013 G4220 81012 64220 20028 64220 20001 0905 1719 G4220 60016 5062 2484 0905 1718 G4220 63010 2x 5067 5678 G4280 60026 01018 22707 5067 4728 Parts and Materials for Maintenance 10 Desc
43. the clips 1 and 2 Insert the pump clips to the holes in the binary pump pull the pump to the front 2 housing 152 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Releasing a Stuck Inlet Valve Releasing a Stuck Inlet Valve If inlet valve is stuck or if pump is not generating pressure after being turned off for an extended When period of time Before the system is turned off for an extended period of time it should be flushed with at NOTE least 10 IPA to prevent inlet valves from getting stuck 1 Remove the capillary connection from the outlet of the 2 Unscrew the tubing at the inlet valve secondary pump head Agilent 1290 Infinity Binary Pump User Manual 153 9 Maintenance Releasing a Stuck Inlet Valve 3 Attach a Luer lock syringe with adapter to the tubing and 4 Reconnect tubing to inlet valve fill it with solvent 5 Unscrew tubing at degassing unit and attach the syringe 6 Push solvent with syringe until it comes out at the top of syringe until it comes out at the top of to it the High Pressure Filter Assembly 154 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Releasing a Stuck Inlet Valve 7 Detach the syringe and reconnect the tubing into the degassing unit 9 Purge the system to remove air 8 Reinstall the capillary connection to the Hig
44. the descriptions in this manual For details refer to the Agilent Lab Advisor software help files Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 7 Error Information What Are ErrorMessages 93 General Error Messages 94 Timeout 94 Shutdown 95 Remote Timeout 96 Lost CAN Partner 97 Leak Sensor Short 97 Leak Sensor Open 98 Compensation Sensor Open 98 Compensation Sensor Short 99 Fan Failed 99 Leak 100 OpenCover 100 Cover Violation 101 Pump Error Messages 102 Pressure of binary pump above upper limit 102 Pressure below lower limit 103 Target pressure not reached for binary pump degasser 103 Degasser s pressure limit violation 104 Solvent counter exceeded limit 104 Waste counter limit exceeded 105 Flow rate limit exceeded 105 Binary pump shutdown during analysis 106 Reading the pump encoder tag failed 106 Writing the pump encoder tag failed 107 Pump drive blocked or encoder failed 107 Drive current too low 108 Drive Encoder failed 108 Ee Agilent Technologies 91 7 92 Error Information Drive current too high 108 Drive timeout 109 Overcurrent of pump drive 109 Overcurrent of solvent selection valve SSV 109 Deliver underrun 110 Defect connection between main board and pump drive encoder 110 Pump drive encoder defect 110 Purge valve failed 111 Reading of purge valve tag failed 111 Pump drive encoder rollover 111 Drive position limit 112 Insufficient power of driv
45. 0 60128 Seals PE seals pack of 2 0905 1420 Wash Seal PE 0905 1718 Agilent 1290 Infinity Binary Pump User Manual 75 4 Using the Pump Normal Phase Applications 76 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 5 Optimizing Performance Delay Volume and Extra Column Volume 78 Delay Volume 78 How to Configure the Optimum Delay Volume 79 How to Achieve Higher Resolution 81 Using Solvent Calibration Tables 84 This chapter gives hints on how to optimize the performance or use additional devices ot Agilent Technologies 77 5 Optimizing Performance Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even though this will have an effect on the chromatography Thi
46. 14 Appendix 293 General Safety Information 294 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 297 Radio Interference 298 Sound Emission 298 Agilent Technologies on Internet 298 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 1 Introduction Features 10 Overview of the Binary Pump 11 Pump Principle 12 System Overview 15 Leak and Waste Handling 15 This chapter gives an introduction to the pump instrument overview and internal connectors Apg Agilent Technologies 1 introduction Features The Binary pump is designed for highest performance GLP compliance and easy maintenance It includes the following features Seal wash for continued high lifetime of pump seals for buffer applications Solvent selection valve for method flexibility Jet Weaver for optimum mixing performance with a minimum of delay volume Automatic purge function for ease of use and unattended preparation of the system Auto tuning of the delivery cycle for compensation of elasticity and solvent volume change effects compressibility thermal expansion mixing contraction Solvent selection for optimum flow and composition accuracy Fast defill function for improved intake and delivery performance Two pistons in series design for increased reliability High resolution piston movement control for smooth and reliable motion For specifications see Table 2 on page
47. 4 Assembling the Pump Head 176 Replacing the Purge Valve Head 188 Replacing Parts of the High Pressure Filter Assembly 191 Installing the Valve Rail Kit 193 Replacing the Main Power Fuses 194 ote Agilent Technologies 127 9 Maintenance Replacing Module Firmware 196 Preparing the Pump Module for Transport 197 This chapter describes the maintenance of the Agilent 1290 Infinity Binary Pump 128 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Introduction to Maintenance Introduction to Maintenance Figure 18 on page 129 shows the main user accessible assemblies of the Agilent 1290 Infinity Binary Pump These parts can be accessed from the front simple repairs and don t require to remove the pump from the system stack Solvent selection valve cm SSV Eo o S _f _ f c i Leam JP P amp Jet Weaver Shutoff valve panel Ee l l xm N Leak funnel and tube f i ressure sensor Degasser 5 l al Left pump head assambl Right pump head assembly Purge valve Figure 18 Maintenance Parts Figure 19 on page 130 shows the flow connections between these main assemblies Agilent 1290 Infinity Binary Pump User Manual 129 9 Maintenance Introduction to Maintenance To autosampler From solvent reservoirs t econda pump head Channel A To waste Figure 19 Flow Connections 130 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Warnings and Cautions WARNING
48. 6 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Tools Alignment piston Tool handle Figure 36 Replacement kit for 1290 Infinity pump head alignment tool piston handle Figure 37 HPLC System Tool Kit Agilent 1290 Infinity Binary Pump User Manual 227 10 Parts and Materials for Maintenance HPLC System Tool Kit The HPLC System Tool Kit G4203 68708 contains some accessories and tools needed for installation and repair of the module p n Description 0100 1681 Adapter syringe seal wash tube 0100 1710 Mounting Tool for Tubing Connections 01018 23702 Insert tool 5023 0240 Hex driver 4 slitted 8710 0060 Hex key wrench 9 64 inch 8710 0510 2x Wrench open 1 4 5 16 inch 8710 0641 Hex key set 1 5 mm 8710 0899 Pozidriv screwdriver 8710 1534 Wrench 4 mm both ends open end 8710 1924 Wrench open 14 mm 8710 2392 Hex key 4 mm15 cm long T handle 8710 2393 Hex key 1 5 mm straight handle 10 cm 8710 2394 Hex key 9 64 inch 15 cm long T handle 8710 2409 Wrench open end 5 16 3 8 inch 8710 2411 Hex key 3 mm12 cm long 8710 2412 Hex key 2 5 mm 15 cm long straight handle 8710 2438 Hex key 2 0 mm 8710 2509 Screwdriver Torx TX8 8710 2594 Double open end wrench 4 mm x 5 mm 9301 0411 Syringe Plastic 9301 1337 Adapter syringe solvent tube with fitting 228 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 11 Identifying Cables
49. 6 are connected to waste capillaries The central port is connected to the Jet Weaver inlet o pressure sensor From pump channe To Jet Weaver o waste 190 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing Parts of the High Pressure Filter Assembly When For removing blockages and leaks in the high pressure filter assembly The filter frit in the outlet valve should be replaced regularly depending on the system usage Other parts are covered by the Agilent Preventive Maintenance PM Service Tools required p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20015 Adapter in square to hex 14 mm Hex bit Parts required p n Description 1 01018 22707 PTFE frits pack of 5 1 5067 4728 Seal cap CAUTION Leakage or damaged connection Opening the outlet of the primary pump head may cause leaks or damage the connection between the pump heads gt Do not open the outlet of the primary pump head This procedure describes replacements for channel A left pump head assembly and can be applied accordingly to channel B In both cases maintenance is done only at the secondary pump head outlet which hosts the filter frit When replacing a PTFE frit consider replacing the seal cap as well in order to prevent leaks Agilent 1290 Infinity Binary Pump User Manual 191 9 Maintenance Replacing Parts of the High Pressure Filter Assembly 1 Remove the capillary connection from the
50. Agilent 1290 Infinity Binary Pump User Manual 209 10 Parts and Materials for Maintenance Pump Head Assembly Parts Pump Head Assembly Parts Figure 26 Pump head assembly parts 210 Agilent 1290 Infinity Binary Pump User Manual Item p n 1 G4280 60026 2 G4220 60028 3 G4220 60022 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Description High Pressure Filter Assembly secondary pump head Outlet valve primary pump head Inlet valve primary pump head 211 10 Parts and Materials for Maintenance Primary Pump Head Parts Primary Pump Head With Seal Wash Binary Pump B Figure 27 Primary pump head Binary Pump with seal wash 212 Agilent 1290 Infinity Binary Pump User Manual Item p n 1 0515 1218 3 0905 1719 4 G4220 60016 5 5062 2484 6 0905 1718 7 G4220 63010 8 9 10 5067 5678 11 G4220 60022 12 G4220 60028 13 G4220 20028 14 G4220 20020 Agilent 1290 Infinity Binary Pump User Manual 10 Parts and Materials for Maintenance Description Screw M5 40 mm long Pump Chamber Housing order pump head PE Seal Seal holder including backup ring Gasket seal wash pack of 6 Wash Seal PE Support Ring Seal Wash Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic Inlet valve primary pump head Outlet valve primary pump head Headless screw for 1290 Infinity pump heads Internal gold
51. Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1290 Infinity Binary Pump User Manual 231 11 Identifying Cables Analog cables 232 One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 7 oe 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog an Center Center Analog Agilent 1290 Infinity Binary Pump User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog a 3 Red Analog S e Agilent 1290 Infinity Binary Pump User Manual 233 11 Identifying Cables Remote Cables 234 O
52. ID 22413 Internal error e Parameter None Probable cause Suggested actions 1 Internal error Please contact your Agilent service representative Defect connection between main board and pump drive encoder Error ID 22414 Defect connection between main board and pump drive encoder e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect connection between main board and Please contact your Agilent service pump drive encoder representative 2 Defect encoder Please contact your Agilent service representative Pump drive encoder defect Error ID 22415 Defect pump drive encoder e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect encoder Please contact your Agilent service representative 110 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Purge valve failed Error ID 22417 Lost steps of the purge valve encoder e Parameter None Probable cause Suggested actions 1 Purge valve drive mechanically blocked or e Check installation of purge valve head defect e Please contact your Agilent service representative Reading of purge valve tag failed Error ID 22420 Reading the purge valve tag failed e Parameter None Probable cause Suggested actions 1 Reading of purge valve tag failed Check cable connection 2 Purge valve head tag defect or empty Replace purge valve head 3 Purge valve tag reader is defect Please contact you
53. No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed 250 Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Table 11 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 2 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector s for signal output Agilent 1290 Infinity Binary Pump User Manual 251 12 Hardware Information 252 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g
54. No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes CAN DC OUT for CAN G4204A Quat Pump slaves G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B K1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B K1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes Agilent 1290 Infinity Binary Pump User Manual 249 12 Hardware Information Table 11 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No
55. ON UV Lamp Tag Use UV lamp anyway Cell Tag Use cell anyway Analog Out 1 OV 1 output range Analog Out 2 OV 1V_ output range UV lamp Stays off at power on LAN Subnet Mask 255 255 248 0 LAN Def Gateway 134 40 24 1 System I Controller DAD I I Figure 63 Instant Pilot LAN Configuration 5 Press the Edit button only visible if not in Edit mode perform the required changes and press the Done button 6 Leave the screen by clicking Exit Agilent 1290 Infinity Binary Pump User Manual 289 13 LAN Configuration PC and User Interface Software Setup Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see also Initialization mode selection on page 266 FIXED IP address Local Area Connection Properties 2 x Internet Protocol General General Connect using You can get IP setings assigned automatically if your network supports this capability Otiierwise you need to ask your network administrator for the appropriate IPiisettings a Xircom CreditCard Ethemet 10 100 Modem 56 C_Dbierrarrtatiireseautomatically Use the following IP address IP adiere 192 168 254 1 Subnet mask 255 255 248 0 Default gateway p Cc Install Uninstall Properties Ji ER Use the folo General Transmission Control Pro
56. P requests box must be unchecked when you have finished configuring instruments otherwise the log file will quickly fill up disk space 3 Click Edit BootP Addresses The Edit BootP Addresses screen appears 4 Click Add The Add BootP Entry screen appears x Mac Address Host Name ae Figure 53 Enable BootP logging 280 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 5 Make these entries for the instrument e MAC address e Host name Enter a Hostname of your choice The Host Name must begin with alpha characters i e LC1260 e IP address e Comment optional e Subnet mask e Gateway address optional The configuration information entered is saved in the Tab File Click OK Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK After each modification of the BootP settings G e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 282 or Restarting the Agilent BootP Service on page 283 oo N OO 10 Power cycle the Instrument OR If you changed the IP address power cycle the instrument for the changes to take effect 11 Use the PING utility to verify connectivity by opening a command window and typing Ping 192 168 254 11 for example The Tab File is located at C Documents and Settings All Users Application Data Agilent BootP TabFile Agilent 1290
57. TR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 40 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1290 Infinity Binary Pump User Manual 253 12 Hardware Information APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addi
58. Wi N h N a be Agilent Technologies Notices Agilent Technologies Inc 2010 2014 2015 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64220 90006 Rev E Edition 02 2015 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardwa
59. a system that is not pressurized for better chance of success Re run test 3 Damaged pump seals or pistons Exchange the pump head or pump seals NOTE A wrongly installed pump seal will reduce the life time of the pump seal considerably 4 Leaky outlet ball valve Exchange pump head or outlet ball valve 5 Leaky purge valve Exchange Purge valve rotor seal Notice the difference between error in the test and a failed result An error is caused by an abnormal termination during the operation of the test whereas a failed result indicates that the test result were not within the specified limits 126 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 9 Maintenance Introduction to Maintenance 129 Warnings and Cautions 131 Overview of Maintenance 133 Cleaning the Module 134 Installing Fittings and Capillaries 135 Replacing the Shutoff Valve Panel 136 Replacing the Pressure Sensor 138 Overview of Torques for Pump Head Procedures 141 Replacing the Inlet valve 142 Replacing the Outlet Valve 144 Replacing the Solvent Selection Valve SSV 147 Changing configuration or replacing the Jet Weaver 149 Replacing the Seal Wash Pump 152 Releasing a Stuck Inlet Valve 153 Replacing the Pump Heads 156 Disassembling the Pump Head 162 Disassembling the Primary Pump Head 163 Disassembling the Secondary Pump Head 167 Replacing the Heat Exchanger 171 Replacing Wash Seal and Gasket 17
60. act Agilent support Drive current too high Error ID 22409 The current consumption of the pump drive is too high Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit purge valve heat exchanger 2 Drive motor defect Please contact your Agilent service representative 108 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Drive timeout Error ID 22410 Drive is blocked mechanically fails during initialization e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Blockage of pump drive Drive motor defect Please contact your Agilent service representative Overcurrent of pump drive Error ID 22411 The current consumption of the pump drive is too high e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit purge valve heat exchanger 2 Drive motor defect Please contact your Agilent service representative Overcurrent of solvent selection valve SSV Error ID 22412 Overcurrent of solvent selection valve SSV Parameter None Probable cause Suggested actions 1 Valve defect Replace the solvent selection valve Agilent 1290 Infinity Binary Pump User Manual 109 7 Error Information Deliver underrun Error
61. ain System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote e error handling diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 244 Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using the following tools latest version should be used Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files on a USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the docu
62. ance specifications 24 performance Optimization 77 physical specifications 23 power considerations 20 power consumption 23 power cords 21 power supply indicator 87 power switch 40 pressure below lower limit 103 pressure exceeded upper pressure limit 102 Agilent 1290 Infinity Binary Pump User Manual Index pressure sensor calibration wrong or missing 117 pressure sensor replace 138 ump drive blocked or encoder ailed 107 ump drive encoder defect 110 ump drive encoder error 115 ay ump drive encoder rollover 111 ump drive error 115 ump drive stop not found 116 ump drive stroke blocked 116 ump error messages 102 Vo 0 US Sa Ve Uo w ump head leak test error message 126 pump features 10 flow connections 43 overview 11 preparing 51 principle 12 purge valve failed 111 reading of purge valve tag failed 111 reading the pump encoder tag failed 106 remote cable 234 repairs replacing firmware 196 resolution Optimization 81 RS 232C cable 240 S safety class 295 safety general information 295 301 Index standards 23 symbols 294 Seal wash option installation 47 seal wash pump was missing when tried to turnon 117 seal wash pump replacing 152 shutdown 95 shutoff valve panel replacing 136 shutoff valves replacing 136 site requirements 19 power cords 21 45 51 solvent counter exceeded limit 104 solvent handling 72 solvent inlet filter 51 solvent cabinet special inte
63. aoc Site IIEL HE Figure 23 Flow connections of the pump Agilent 1290 Infinity Binary Pump User Manual Item p n 1 5067 4661 2 5067 4655 2x 3 5067 4656 2x 4 G4220 87000 5 5042 8507 5065 9978 6 G4220 40004 5067 4124 64220 60035 G4220 60007 G4220 68070 G4220 60070 G4220 60017 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Description Tubing kit 270 mm for connection of degassing unit to inlet valve set of 2 tubes Capillary ST 0 25 mm x 235 mm purge valve to pump head assemblies channel A and B 2x Capillary ST 0 25 mm x 80 mm purge valve to pressure sensor 2x Capillary ST 0 17 mm x 300 mm Valve to Jet Weaver Seal wash pump cartridge Tubing 1 mm i d 3 mm o d silicone 5 m Shutoff valve panel Shutoff valve Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit Bottle Head Assembly Ultra Clean Tubing Kit for G4220A includes bottle head assemblies and tubing connections within the pump Tubing Kit 140 mm Ultra Clean Tubing tubes from SSV to shutoff valve or degassing unit to MCGV Bottle Head Assembly Ultra Clean Tubing bottle heads and tubing to shutoff panel degasser 205 10 Parts and Materials for Maintenance Solvent Cabinet Kit Item p n 1 5067 4644 2 5065 9981 3 9301 1420 3x 4 9301 1450 5 5067 4124 4x 6 G4220 60007 4x 7 G4220 60035 4x 8 G4220 40004 9 5042 9967 206 Descr
64. ase Applications 74 Agilent 1290 Infinity Binary Pump User Manual Contents Optimizing Performance 77 Delay Volume and Extra Column Volume 78 How to Configure the Optimum Delay Volume 79 How to Achieve Higher Resolution 81 Using Solvent Calibration Tables 84 Troubleshooting and Diagnostics 85 Overview of the Module s Indicators and Test Functions 86 Status indicators 87 Available Tests vs User Interfaces 89 Agilent Lab Advisor Software 90 Error Information 91 What Are Error Messages 93 General Error Messages 94 Pump Error Messages 102 Test Functions and Calibrations 119 Introduction 120 System Pressure Test 121 Pump Head Leak Test 124 Maintenance 127 Introduction to Maintenance 129 Warnings and Cautions 131 Overview of Maintenance 133 Cleaning the Module 134 Installing Fittings and Capillaries 135 Replacing the Shutoff Valve Panel 136 Replacing the Pressure Sensor 138 Overview of Torques for Pump Head Procedures 141 Replacing the Inletvalve 142 Replacing the Outlet Valve 144 Replacing the Solvent Selection Valve SSV 147 Changing configuration or replacing the Jet Weaver 149 Replacing the Seal Wash Pump 152 Agilent 1290 Infinity Binary Pump User Manual Contents Releasing a Stuck Inlet Valve 153 Replacing the Pump Heads 156 Disassembling the Pump Head 162 Disassembling the Primary Pump Head 163 Disassembling the Secondary Pump Head 167 Replacing the Heat Exchanger 171 Replacing Wash Seal a
65. aste The condense drain outlet of the autosampler cooler 5 guides condensate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container Agilent 1290 Infinity Binary Pump User Manual 17 1 introduction System Overview 18 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 20 Physical Specifications 23 Specifications 24 This chapter provides information on environmental requirements physical and performance specifications ee Agilent Technologies 19 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Consideration The module power supply has wide ranging capabilities and accepts any line voltage in the range mentioned in Table 1 on page 23 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power
66. ation 126 This chapter describes the tests for the module Apg Agilent Technologies 119 8 Test Functions and Calibrations Introduction All tests are described based on the Agilent Lab Advisor Software B 01 04 Other user interfaces may not provide any test or just a few Table9 Interfaces and available test functions Interface Comment Available Function Agilent Instrument Utilities Maintenance tests available System pressure test Agilent Lab Advisor All tests are available System pressure test Pump head leak test Agilent ChemStation No tests available Pressure Adding of pressure to e Pressure ripple chromatographic signals Temperature main board possible Agilent Instant Pilot Some tests are available System pressure test Monitoring of values Pressure Pressure ripple Flow in case of operating pressure For details on the use of the interface refer to the interface documentation 120 Agilent 1290 Infinity Binary Pump User Manual Test Functions and Calibrations 8 System Pressure Test The test determines the leak rate of the system between pump outlet valves and a blank nut The blank nut can be positioned at different locations in the system before the flow cell to determine and verify the leak rate of individual modules and components The test allows for setting the pressure at which the test is performed The leak rate of high pressure parts is not always a linear function and therefore
67. ble Tests vs User Interfaces 89 Agilent Lab Advisor Software 90 Overview about the troubleshooting and diagnostic features Apg Agilent Technologies 85 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations Diagnostic Signals The module has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems These can be added like normal signals in the Agilent ChemStation software 86 Agilent 1290 Infinity Binary Pump User Manual Troubleshooting and Diagnostics 6 otatus indicators Status indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right in
68. ction between the power switch board and the main board 2 Defective power switch board 3 Defective main board Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective power switch board Please contact your Agilent service representative 2 Loose connection between the power Please contact your Agilent service switch board and the main board representative 3 Defective main board Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the
69. d Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 s This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex l this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Agilent 1290 Infinity Binary Pump User Manual 297 14 Appendix Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operato
70. d LAN cable MAC label LAN interface a iat m m ja ia im O m a oO m p0880060600666 Figure 43 Location of LAN interfaces and MAC label Agilent 1290 Infinity Binary Pump User Manual 263 13 LAN Configuration TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 271 e by manually setting the parameters using Telnet e by manually setting the parameters using the Instant Pilot G4208A The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 18 on page 266 264 Agilent 1290 Infinity Binary Pump User Manual
71. d and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCly 02 gt 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong ba
72. dicates the module status i ae LI Power switch i oY F lt Serial number Figure 13 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Agilent 1290 Infinity Binary Pump User Manual 87 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 88 When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different
73. e gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 22 Agilent 1290 Infinity Binary Pump User Manual Site Requirements and Specifications 2 Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 21 8 kg 48 Ibs Dimensions height x width 240 x 345 x 435 mm 9 3 x 13 5 x 17 x depth inches Line voltage 100 240 V 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 350 VA 270 W 922 BTU Maximum Ambient operating 4 55 C 39 131 F temperature Ambient non operating 40 70 C 40 158 F temperature Humidity lt 95 r h at 40 C 104 F Non condensing Operating altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15092 ft For storing the module Safety standards IEC CSA Installation category Il Pollution degree2 For indoor use only UL Agilent 1290 Infinity Binary Pump User Manual 23 2 Site Requirements and Specifications Specifications Table 2 Performance specifications Type Specification Comments Hydraulic system Settable flow range Flow precision Flow accuracy Pressure operating range Pressure pulsation Compressibility compensation Gradient formation Delay volume Composition range Composition precision Composition accuracy Two dual pistons in seri
74. e typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a pH range between 1 12 and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra
75. e STDC gt 9 2 z 2 c T e __9Cc TG i Configuration switch f NENG ee A 5 a oe COMPACT FLASH ower supply for externa a j CAN valves CAN DC 0UT CAH CAN E USB w ca ED ee C a2 ED en eID Ce eZ Oa pas ZZ CED aoe Sy ZB CLC SSE ZS CERE CDQ eza gt TID CW l DC SS z 20D gt gt 2c Ss amy IC IC gt A gt C IC D Z gt IC IC IC E Sa 3 C gt a 3 C3 AN T gt fe C 3 gt c i gt a fe Na dC c S 5 fa p c aye gt lt 3 C d SC a Cy C C 5C c J c ae c T gt Te E i 955 oO Compact Flash IC ye ye gt c 5 c c 3 c S lt ate SE gt fi SC lt 5 Se IC p aN IC D gt A pia C 2 PIAN DC DC C ri gt CS DC bie b D amp gt C Z D x CAC DC C iD Be SS Zo IDT Q ar a a CO oO ama SS SB aS 42 GBMmaaonpasas SS 6 SE fen Z SS Spaz O CS Sees i o l T E ilem Technologies Q cemeg 250VACG TIOAH SOE aie ena fem EET IM ww fe frm 1 TMU MUTANT MOM sxe Made in Germany lsat Power plug Figure 39 248 Rear of Binary Pump Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 11 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes
76. e encoderLED 112 Drive encoder error 112 Writing the purge valve tag failed 113 Current of primary pump drive too high 113 Current of secondary pump drive too high 114 Unknown purge valve type 114 Pump drive encoder error 115 Pump driveerror 115 Pump drive stroke blocked 116 Pump drive stop notfound 116 Pressure sensor calibration wrong or missing 117 Seal wash pump was missing when tried to turnon 117 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1290 Infinity Binary Pump User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on i
77. e5 L o l m oO G Comey ase mnb icasi amn 250VAG TIOAH Figure 10 Rear of Binary Pump In an Agilent 1290 Infinity System the individual modules are connected by CAN cables An Agilent 1200 Series Instant Pilot can be connected to the CAN bus of any module Connection to an Agilent data system is established through the built in LAN port of the detector The LAN port of the detector must be used as the detector generates the highest data rate of all modules For more information about connecting the Instant Pilot or Agilent Data System please refer to the respective user manual For setting up the LAN access see LAN Configuration on page 261 Agilent 1290 Infinity Binary Pump User Manual 41 3 42 Installing the Module 5 Turn on the power by pushing the button at the lower left hand side of the module The power button stays pressed in and the status LED should be green When the line power button stands out and the green light is off the module is turned off The module was shipped with default configuration settings For changing these settings refer to section Setting the 8 bit configuration switch Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 Flow connections to the pump Flow connections to the pump Jet Weaver Degasser T aA Solvent selection valve TEN N m Y
78. ecification Comments Solvent selection valve Default Standard part of the pump Integrated degassing unit Number of channels 2 Internal volume per channel 1 5 mL Control Agilent ChemStation for LC B 04 02 or above EZChrom Elite 3 3 2 SP1 or above OpenLAB 3 3 2 SP3 Masshunter B 02 01 SP1 or above Local control Agilent Instant Pilot G4208A B 02 08 or above Communications Controller area network CAN RS232C APG remote ready start stop and shutdown signals LAN Safety and maintenance Extensive diagnostics error detection and display through Agilent Lab Advisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas GLP features Early maintenance feedback EMF for continuos tracking of instrument usage in terms of purge valve switches and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclable Agilent 1290 Infinity Binary Pump User Manual 25 2 Site Requirements and Specifications Specifications 26 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 3 Installing the Module Unpacking the Module 28 Optimizing the Stack Configuration 30 One Stack Configuration 30 Two Stack Configuration 33 Installation Information on Leak and Waste Handling 35 Removing the Transp
79. ed time Periodic a periodic wash will be applied for a defined period in minutes The option is available only when the pump has seal wash capability The seal wash capability is detected by the module itself If seal wash is installed it is recommended to use it in order to increase the primary seal lifetime Allows you to define when to use the seal wash Off The seal wash is inactive On when pump is on The seal wash is active only when the pump is on On all the time The seal wash is active when the pump is on or in standby mode Module can be turned on at a specified date time This feature can only be used if the module power switch is turned on 58 Agilent 1290 Infinity Binary Pump User Manual Table4 Pump control parameters Using the Pump 4 Parameter Limits Description Purge Time 0 100 00 min Setup and activation of Purge parameters The automatic purge valve can in steps of 0 01 be used for purging the system The process has been automated for ease Flow 0 000 of use 5 000 mL min for Off Turns off the purge each channel in steps of 0 001 10 000 mL min maximum Prime Conditioning at least 200 bar gt 500 bar is better On The device is purged Purge Flow Time and Composition during purge have to be defined As soon as the duration time of the purge ends the module automatically switches to analytical conditions again Select On to start priming Of
80. een 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Agilent 1290 Infinity Binary Pump User Manual 237 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 238 Agilent 1290 Infinity Binary Pump User Manual CAN LAN Cable Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Agilent 1290 Infinity Binary Pump User Manual 239 11 Identifying Cables RS 232 Cable Kit p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5
81. enance 10 To dry the pressurize 170 pump head an d air 1 2 a d to remove residues use nd 3 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Heat Exchanger Tools required p n Description Wrench 19 mm 5023 2501 Screwdriver Torx T10 Parts required p n Description G4220 81013 Heat Exchanger secondary pump head only Channel A G4220 81012 Heat Exchanger secondary pump head only Channel B G4220 20028 Headless screw for 1290 Infinity pump heads G4220 20001 Spacer Fitting Preparations Remove the pump head assembly from the pump Remove the secondary pump head from the link plate CAUTION Loss of small spacer fitting Inside the secondary pump head is a small spacer fitting which can be dropped easily when removing the heat exchanger gt The heat exchanger does not need to be removed for pump head maintenance Agilent 1290 Infinity Binary Pump User Manual 171 9 Maintenance Replacing the Heat Exchanger 1 Remove the 19 mm screw at the front of the secondary pump head 2 Remove the front plate 3 Remove the screw at the bottom of the pump head Do not drop the golden spacer fitting Screw 4 Lift out the heat exchanger 172 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Heat Exchanger 5 If removed first insert the spacer fitting Then insert the new heat exchanger to the o
82. ent Revison _B 06 40 0007 A print On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 LAN MAC Address _0030D314F89E Board ID TYPE G1315 66565 SER MAC REV AC MFG Lamp 2140 0820 848728 Cell no info Exit v information on each module a 10 08 Figure 50 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules Table 20 G1369C LAN Interface Card configuration switch on the card SW 4 SW 5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP Agilent 1290 Infinity Binary Pump User Manual 271 13 LAN Configuration 272 Table 21 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation Lab Advisor Firmware Update Tool and use MAC address as host name e g 003043177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 270 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting netw
83. entry or select an existing line from the table and press Modify or Delete to change the IP address comment subnet mask for example in the Tab File If you change the IP address it will be necessary to power cycle the instrument for the changes to take effect 5 Leave Edit BootP Addresses by pressing Close 6 Exit BootP Settings by pressing OK Restarting the Agilent BootP Service 1 In the Windows control panel select Administrative Tools gt Services The Services screen appears see Figure 54 on page 282 2 Right click Agilent BootP Service and select Start 3 Close the Services and Administrative Tools screens Agilent 1290 Infinity Binary Pump User Manual 283 13 LAN Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 56 Manual Configuration Principle 284 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters set by any method the parameters may be altered by opening a Telne
84. ervice main board representative 2 Missing or defect tag Defect connection Please contact your Agilent service between tag and encoder representative 106 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Writing the pump encoder tag failed Error ID 22405 Writing the pump encoder tag has failed e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent service main board representative 2 Defect tag Defect connection between tag Please contact your Agilent service and encoder representative Pump drive blocked or encoder failed Error ID 22406 Pump drive blocked or encoder failed e Parameter None Probable cause Suggested actions 1 Blockage of the pump drive Drive encoder Please contact your Agilent service failed representative Agilent 1290 Infinity Binary Pump User Manual 107 7 Error Information Drive current too low Error ID 22407 The current consumption of the pump drive is too low Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Drive motor defect Please contact your Agilent service representative 2 Wrong missing connection of pump drive to Please contact your Agilent service main board representative Drive Encoder failed Error ID 22408 Drive encoder failed during pump drive calibration Probable cause Suggested actions 1 Internal error Cont
85. ervoirs Disconnect the solvent tubing from the inlet of primary pump heads for both solvent channels Use a syringe for removing liquid from the solvent tubings between solvent reservoir shutoff valve panel solvent selection valve degassing unit and pump inlets Switch the solvent selection valve if applicable Inlet primary pump head 2 Remove tubing and capillary connections to other modules and the solvent cabinet Remove tubing plugs Jet Weaver Outlet to Autosampler A Al Inlet Automatic Seal Wash optional behind shutoff valve panel B1 B2 m ZEX ALE Waste tubes Purge Valve Outlet Active Seal Wash optional 198 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Preparing the Pump Module for Transport 3 Remove the shutoff valve panel by pulling it downwards 4 You may keep internal tubing and capillary connections Jet Weaver Solvent selection valve Pump heads Purge valve Pressure sensor 5 Remove cable connections to other modules Remove the module from the stack Agilent 1290 Infinity Binary Pump User Manual 199 9 Maintenance Preparing the Pump Module for Transport 6 Carefully insert the Protective Foam to the front part of the instrument Do not damage any tubing or capillary connections 7 Close the front cover 8 For transport or shipment put the module and acce
86. es are available on the Internet Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets Agilent 1290 Infinity Binary Pump User Manual 35 3 Installing the Module Installation Information on Leak and Waste Handling Figure 7 Leak and waste handling overview typical stack configuration as an example 36 Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 1 Solvent cabinet Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the sampler s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 7 on page 36 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect capillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual WARNING Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off
87. es pumps with proprietary servo controlled variable stroke design power transmission by ball screws smooth motion control of pistons for active damping Setpoints 0 001 5 ml min in 0 001 ml min increments lt 0 07 RSD or 0 005 min SD whatever is greater 0 2 5 0 ml min 1 or 10 pl min whatever is greater Operating range up to 120 MPa 1200 bar up to 2 ml min 80 MPa 800 bar at 5 ml min lt 1 amplitude or lt 0 5 MPa 5 bar whatever is greater Automatic pre defined based on mobile phase selection High pressure binary mixing Jet Weaver V35 lt 45 ul Jet Weaver V100 lt 75 pl Settable range 0 100 lt 0 15 RSD or 0 01 min SD whatever is greater 0 35 absolute 5 95 0 2 5 0 ml min Executed in 300 pl step increments Based on retention time at constant room temperature Pumping degassed H20 at 10 MPa 100 bar At 1 ml min water JetWeaver generally recommended For applications requiring lowest delay volumes JetWeaver can be removed out of the flow path Delay volume is then solely determined by the volume of the connection capillary Recommended range 1 99 or 5 pl min per channel whatever is greater 0 2 5 0 ml min based on retention time at constant room temperature Water caffeine tracer 24 Agilent 1290 Infinity Binary Pump User Manual Site Requirements and Specifications 2 Table 2 Performance specifications Type Sp
88. ess gt 134 40 27 95 Subnet Mask gt 255 255 248 0 Def Gateway 134 409 24 1 stored IP Address 134 40 27 99 255 255 248 0 Gateway 134 40 24 1 Controllers no connections Figure 61 Telnet Change IP settings Agilent 1290 Infinity Binary Pump User Manual change of IP setting to Initialization mode is Using Stored active TCP IP settings stored TCP IP settings in non volatile memory connected to PC with controller software e g Agilent ChemStation here not connected 287 13 LAN Configuration 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters cx WINDOWS system32 cmd exe Agilent Technologies G4296A DEGHBH0006 gt exit Connection to host lost C V a Figure 62 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 134 40 27 99 288 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 With the Instant Pilot G4208A To configure the TCP IP parameters before connecting the module to the network the Instant Pilot G4208A can be used 1 From the Welcome screen press the More button 2 Select Configure 3 Press the DAD button 4 Scroll down to the LAN settings 7 Configure DAD lt Not Set gt Temperature Control
89. f to turn priming off The Prime function is helpful for filling empty solvent lines or if air has entered the pump heads The module draws solvent at high speed with all four pump drives simultaneously and dispenses it against the waste position of the automatic purge valve This is done 20 times before the process comes to an end Use this function if you see excessive pressure or composition ripple and you are sure that the solvent type aqueous organic or specific solvent solvent mix is correctly set and there is no evidence of leakage in the pump Conditioning may be necessary if the pump may contain air for example after running out of solvent after a long period of standby or after service or repair Agilent 1290 Infinity Binary Pump User Manual 59 4 Using the Pump Method Parameter Settings The Binary Pump method setup parameters are in eight sections e Flow Solvents A and B Stoptime e Posttime e Pressure Limits Timetable Advanced e External Contacts Table5 Method parameters Parameter Limits Description Flow Solvents A and B 0 00 5 00 mL min The flow is the rate of movement of eluent along the column It is in steps of 0 001 important that the flow rate is kept constant to ensure precise retention Recommended flow time and peak measurements Variations in flow rate can occur as a result range 0 05 of the partial failure of the pumping system air in the pumping
90. fe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed Agilent 1290 Infinity Binary Pump User Manual 1 1 Introduction System Overview Figure 2 Leak and waste handling concept overview typical stack configuration as an example 16 Agilent 1290 Infinity Binary Pump User Manual Introduction 1 The solvent cabinet 1 is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet The leak pan 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 8 A guides excessive overfill from one module to the next as the solvent flows into the next module s leak funnel 8 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to w
91. formance of the module and seal life time For performance a low stroke volume is beneficial as it divides disturbances into smaller packages but a larger volume is extending the life time of the pump seals If Automatic is activated the pump tries to achieve an optimized stroke volume for the Jet Weaver geometry Synchronized Select this option to synchronize the strokes for both channels the values for Channel B are set to the same as those for Channel A This is done to avoid floating disturbances affecting instrument performance The compressibility of the mobile phase has an effect on the performance of the pump For best flow accuracy and mixing performance you can set the parameter according to the mobile phase being used Use solvent types e Clear this check box to display the compressibility fields which allow you to enter compressibility values When the check box is selected the compressibility fields are not displayed and the enhanced compressibility calibration is enabled Select the required calibrated solvents from the drop down lists using the combo boxes in the Solvents section You can set a limit on the rate of change of the solvent flow to protect your analytical column You can set individual values for Flow ramp up and Flow ramp down 62 Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Timetable Settings Use the Timetable to program changes in the pump parameters during the anal
92. h Pressure Filter Assembly Agilent 1290 Infinity Binary Pump User Manual 155 9 Maintenance Replacing the Pump Heads Replacing the Pump Heads The Agilent 1290 Infinity Binary Pump has two pump assemblies for two solvent channels A and B which both consist of two pump drives and pump heads The solvent enters each pump through the primary pump head is transferred to the secondary pump head and leaves the outlet of the secondary pump head which is connected to the pump valve Figure 21 Pump head assembly left 156 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 In case of problems with the pump performance p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20015 Adapter in square to hex p n Description 1 G4220 60200 Pump Head Assembly Channel A left with Seal Wash Option 1 G4220 60210 Pump Head Assembly Channel B right with Seal Wash Option 1 G4220 60400 Pump Head Assembly Channel A left without Seal Wash Option 1 G4220 60410 Pump Head Assembly Channel B right without Seal Wash Option Switch off pump at the main power switch Remove the front cover Limitation of life time The pump head assembly is an exchange part which cannot be reassembled with standard tools Disassembling the pump head will strongly limit its life time gt Do not disassemble the pump head assembly Damage of connections Disassembling the flow con
93. ication 275 Installation of BootP Service 276 Two Methods to Determine the MAC Address 278 Assigning IP Addresses Using the Agilent BootP Service 279 Changing the IP Address of an Instrument Using the Agilent BootP Service 282 Manual Configuration 284 With Telnet 285 With the Instant Pilot G4208A 289 PC and User Interface Software Setup Setup 290 PC Setup for Local Configuration 290 User Interface Software Setup 291 This chapter provides information on connecting the detector to the Agilent ChemStation PC oh Agilent Technologies 261 13 LAN Configuration What You Have to Do First The module has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module see Figure 43 on page 263 G42204 65600 4587 Part number of the pump main board B 01 XX 0925 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Country of Origin Figure 42 MAC Label 262 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 What You Have to Do First 2 Connect the instrument s LAN interface see Figure 43 on page 263 to the PC network card using a crossover network cable point to point or a hub or switch using a standar
94. ifically this can be the case when UV detection is employed and a strongly UV absorbing compound has been added to the mobile phase This can have the effect of emphasizing any pump noise and the most common example is the use of trifluoroacetic acid TFA in the analysis of proteins and peptides The effect can be mitigated by increasing the mixer volume The following different Jet Weaver configurations are available The Jet Weaver 35 uL 100 uL G4220 60006 has two alternative volumes in the same unit The switch from the lower volume 35 ul to the higher volume 100 ul is done by uninstalling it turning it around from front to back and re installing it see Changing configuration or replacing the Jet Weaver on page 149 The mixing volume and hence delay volume is increased by 65 ul and the baseline performance with additives like TFA will be improved The configuration of the Jet Weaver is logged automatically by an attached RFID tag The 380 uL Jet Weaver high performance mixer is optionally available for demanding applications which use solvents in different channels for example A versus B that differ strongly in their UV Vis absorption for example by using trifluoroacetic acid TFA as a modifier which has a high absorbance Agilent 1290 Infinity Binary Pump User Manual 79 5 Optimizing Performance Solvent packages created by the pump may persist until the solvent reaches the detector flow cell Absorption fluctuat
95. iguration Switch with on board LAN Mode Function sW 1 SW 2 SW 3 SW 4 SW 5 SW6 SW7 SW 8 LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 x x Xx xX X 10 MBit half duplex 1 0 0 x xX X 10 MBit full duplex 1 0 1 x xX X 100 MBit half duplex 1 1 0 x X xX 100 MBit full duplex 1 1 1 x X x Bootp x Xx Xx 0 0 0 Bootp amp Store Xx Xx x 0 0 1 Using Stored x x Xx 0 1 0 DHCP Xx Xx xX 1 0 0 Using Default x X x 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 x Revert to Default Data Coldstart x x Xx 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 258 Agilent 1290 Infinity Binary Pump User Manual 257 12 Hardware Information Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions
96. ings 2 In BootP Settings check Do you want to log BootP requests to enable logging Logging V Do you want to log bootP requests BootP Log File Ea EP Figure 52 Enable BootP logging The log file is located in C Documents and Settings All Users Application Data Agilent BootP LogFile It contains a MAC address entry for each device that requests configuration information from BootP 3 Click OK to save the values or Cancel to discard them The editing ends 4 After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 282 or Restarting the Agilent BootP Service on page 283 5 Uncheck the Do you want to log BootP requests box after configuring instruments otherwise the log file will quickly fill up disk space Determining the MAC address directly from the LAN Interface card label 1 Turn off the instrument 2 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module See Figure 42 on page 262 and Figure 43 on page 263 3 Turn on the instrument Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 Assigning IP Addresses Using the Agilent BootP Service The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address Determining the MAC address of the ins
97. installation lsopropanol Best solvent to flush air out of the system When switching between reverse phase and Isopropanol lsopropanol is miscible with both normal phase normal phase both times and reverse phase solvents After an installation Ethanol or Methanol Alternative to lsopropanol second choice if no lsopropanol is available To clean the system when using buffers Bidistilled water Best solvent to re dissolve buffer cristals After a solvent change Bidistilled water Best solvent to re dissolve buffer cristals Before turning off system for an extended Organic or 10 period of time isopropanol in water The pump should never be used for priming empty tubings never let the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 64 Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 If the system has been run dry or air has diffused into the pump it might require additional steps to get rid of the air again Following the procedure below will give the best and fastest results 1 Change solvent to isopropanol on both channels 2 Turn on the Prime function 3 Purge the system with 10 ml composition 50 50 and for 10 min 4 Attach a column suitable for isopropanol and set the Max pressure limit to the limit of the column 5 Run the system at composition 50 50 and a flow rate that gives a pressure close to the limit of the
98. ions can then show up as baseline noise also referred to as mixing noise Applications like impurity quantitation or lowest level compound detection require minimizing this noise The 380 uL Jet Weaver strongly improves mixing and therefore reduces baseline noise and improves sensitivity in detection Patented Agilent microfluidic technology offers high mixing performance at a low internal volume of 380 uL 80 Agilent 1290 Infinity Binary Pump User Manual Optimizing Performance 5 How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section explains how resolution can be increased by examining the following points e Optimize selectivity e Smaller particle size packing e Longer Columns e Shallower gradients faster flow Resolution between two peaks is described by the resolution equation 1 a 1 k Rs VN 2 i 4 a k where R resolution e N plate count measure of column efficiency e o selectivity between two peaks e ko retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selec
99. iption Solvent Cabinet Kit 1290 Infinity Pump includes the following parts Solvent cabinet 1200 Infinity including all plastic parts Solvent bottle transparent Solvent bottle amber Shutoff valve Bottle Head Assembly Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit Shutoff valve panel Tubing clip set of 5 clips Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Seal Wash Option Figure 24 Seal wash pump p n Description 5042 8507 Seal wash pump cartridge 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5 m Agilent 1290 Infinity Binary Pump User Manual 207 10 Parts and Materials for Maintenance Pump Head Assemblies Figure 25 Pump head assembly left with seal wash option Figure 25 on page 208 exemplarily shows the pump head with seal wash option for channel A of the pump There are several pump heads available see Table 10 on page 209 208 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Table 10 Pump head assemblies Pump Head Assembly Channel A Pump Head Assembly Channel B left with Seal Wash Option right with Seal Wash Option G4220 60200 G4220 60210 New parts Pump Head Assembly Channel A Pump Head Assembly Channel B left without Seal Wash Option right without Seal Wash Option G4220 60400 G4220 60410 All pump head assemblies are complete including valves and filter assemblies
100. it is recommended to perform the test at a pressure that corresponds to the normal operating pressure of the system When In case of a suspected leak To verify successful execution of maintenance tasks Parts required p n Description 1 01080 83202 Blank nut Preparations Solvents must be present in both channels Agilent 1290 Infinity Binary Pump User Manual 121 8 Test Functions and Calibrations System Pressure Test 1 Run the System Pressure Test with the recommended user interface for further information see Online Help of user interface Limits Signals Test Name System Pressure Test Description No description Module 642204 LPO0000005 Approx Time 5 min Status Running E Test Procedure Result Y Name Value 1 Repare pump pressure tesi Maximum system pressure 1200 bar Y Enter the test pressure Channel A2 Done A Configure purge process and parameter Remaining purge time 0 min 0 sec y a gt Insert blank nut System checking leak rate of system Evaluate results Nn Po DMN Restore system configuration Figure 14 System Pressure Test Result System Pressure Test Enter the test pressure Enter the pressure at which the test will be executed 1 200 bar Figure 15 System Pressure Test dynamic pressure input 122 Agilent 1290 Infinity Binary Pump User Manual Test Functions and Calibrations 8 System Pressure Test Evaluation Test
101. ith concentrations of 0 1 M or higher are being pumped for long periods of time Never leave an unused pump with water in a channel for an extended period of time 2 3 days Always flush with organic solvent or add 10 isopropanol to water Agilent 1290 Infinity Binary Pump User Manual 51 4 Using the Pump Algae Growth in HPLC Systems 52 The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure e Column filters to plug giving high system pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common Agilen
102. ive stop not found Error ID 22442 The maximum stroke is too long e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Wiper shifted Please contact your Agilent service representative 2 Pump drive spindle is defect Please contact your Agilent service representative 116 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Pressure sensor calibration wrong or missing Error ID 22443 Pressure sensor calibration wrong or missing Parameter None Probable cause Suggested actions 1 Pressure sensor calibration wrong or e Replace pressure sensor missing e Please contact your Agilent service representative Seal wash pump was missing when tried to turn on Error ID 22499 The seal wash pump has not been detected while being configured or detected before Probable cause Suggested actions 1 Defect cable connection to seal wash pump Check cable connection 2 Defect seal wash pump motor Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual 117 7 Error Information Pump Error Messages 118 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 8 Test Functions and Calibrations Introduction 120 System Pressure Test 121 System Pressure Test Evaluation 123 Pump Head Leak Test 124 Pump Head Leak Test Evalu
103. kup ring Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic High Pressure Filter Assembly secondary pump head PTFE frits pack of 5 Seal cap 219 10 Parts and Materials for Maintenance Purge Valve Purge Valve Figure 31 Purge valve parts 220 Agilent 1290 Infinity Binary Pump User Manual Item p n 5067 4119 1 1535 4857 2 5068 0004 3 5068 0005 4 1535 4045 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Description Purge valve head Stator screws Purge Valve Stator Purge Valve Rotor Seal polyimide 1200 bar Bearing ring 221 10 Parts and Materials for Maintenance Cover Parts Cover Parts Figure 32 Cover Parts Figure 33 Tubing plug and tubing grommet Tubing grommet Tubing plug 222 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Item p n Description 1 5067 4613 Cabinet Kit Side Covers left right Top Cover Tubing Plug Base Cover and Leak Seal 2 5042 9949 Tubing Plug Plastic 5042 9972 Tubing grommet 3 5067 4612 Front Cover 1290 Infinity Binary Pump 4 5067 4634 Valve Rail Kit Agilent 1290 Infinity Binary Pump User Manual 223 10 Parts and Materials for Maintenance Leak Parts Figure 34 Leak funnel p n Description 5041 8388 Leak funnel Fuses Item p n Description 1 2110 1004 2x Fuse 10 At 224 Agilent 1290 Infinity Binary Pump User Manual
104. l equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 132 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Overview of Maintenance The following pages describe maintenance simple repairs of the module that can be carried out without opening the main cover Agilent 1290 Infinity Binary Pump User Manual 133 9 Maintenance Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 134 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Installing Fittings and Capillaries WARNING Solvent can spray under high pressure gt Observe appropriate safety procedures for example goggles safety gloves and protective clothing when opening flow path CAUTION Deformation of fittings and seals Liquid drops under high pressure up to 1200 bar act like solid parts Tightening connections under high pressure can deform or destroy fittings and seals gt Never tighten flow connections under pressure The lifetime of a fitting depends on
105. lt X X X X gt sets new subnet mask gw lt X X X x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 286 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 5 Use the and press Enter to list the current settings E C WINDOWS system32 cmd exe telnet 134 40 27 95 gt LAN Status Page MAC Addre 6636D36A8838 Init Mode Using Stored TCP IP Properties active IP Address gt 134 40 27 95 Subnet Mask gt 255 255 248 0 Gateway 134 40 24 1 Controllers no connections Figure 60 Telnet Current settings in Using Stored mode information about the LAN interface MAC address initialization mode Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to PC with controller software e g Agilent ChemStation here not connected 6 Change the IP address in this example 134 40 27 99 and type to list current settings AWINDOWS system32 cmd_exe telnet 134 40 27 95 gt ip 134 40 27 99 gt LAN Status Page MAC Addre 6636D36AG838 sing Stored TCP IP Properties active IP Addr
106. m your Agilent ChemStation DVD located in folder BootP About Agilent BootP Service The Agilent BootP Service is used to assign the LAN Interface with an IP address The Agilent BootP Service is provided on the ChemStation DVD The Agilent BootP Service is installed on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN The BootP service must be running TCP IP network protocol and cannot run a DHCP server 274 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 How BootP Service Works When an instrument is powered on an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides its hardware MAC address as an identifier The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument The instrument receives its IP address and host name and maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent BootP Service must be running every time the instrument powers up If the Agilent BootP Service runs in the background the instrument will receive its IP address on power up The Agilent LAN Interface can be set to store the IP address and will not lose the IP address if power cycled Situation Cannot Establish LAN Communication If a LAN co
107. mage the pump head gt For handling the torque wrench setting and applying k the right torque consult the manual of your torque wrench ESNA D 13 For the secondary pump head assemble and install the COK Ar A high pressure filter assembly using the torque wrench Sy 14 mm hex wrench which is included to the 1290 E 2 A Infinity Service Kit p n 5067 4699 set to 16 Nm T 184 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Assembling the Pump Head 15 Lubricate the pump piston with isopropanol or another 16 Insert both pump heads to the link plate and make sure suitable solvent and insert it that the clips snap in that fix the pump heads NOTE Observe the correct orientation of the primary and the secondary pump head This is important for correct fixation of the heat exchanger and the capillaries as described in the following steps Link plate Agilent 1290 Infinity Binary Pump User Manual 185 9 Maintenance Assembling the Pump Head 17 Bring the pump drive to the maintenance position using the Lab Advisor user interface see Disassembling the Pump Head on page 162 Both pump drives must be retracted Correct Z ZB A A A g A A A A A A A A A CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your to
108. mentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 38 Firmware Update Mechanism Agilent 1290 Infinity Binary Pump User Manual 245 12 Hardware Information 246 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data t
109. mmunication with BootP service cannot be established check the following on the PC e Is the BootP service started During installation of BootP the service is not started automatically Does the Firewall block the BootP service Add the BootP service as an exception e Is the LAN Interface using the BootP mode instead of Using Stored or Using Default modes Agilent 1290 Infinity Binary Pump User Manual 275 13 LAN Configuration 276 Installation of BootP Service Before installing and configuring the Agilent BootP Service be sure to have the IP addresses of the computer and instruments on hand 1 2 3 Log on as Administrator or other user with Administrator privileges Close all Windows programs Insert the Agilent ChemStation software DVD into the drive If the setup program starts automatically click Cancel to stop it 4 Open Windows Explorer 5 Go to the BootP directory on the Agilent ChemStation DVD and double click BootPPackage msi If necessary click the Agilent BootP Service icon in the task bar The Welcome screen of the Agilent BootP Service Setup Wizard appears Click Next The End User License Agreement screen appears Read the terms indicate acceptance then click Next The Destination Folder selection screen appears Install BootP to the default folder or click Browse to choose another location Click Next The default location for installation is C Program Files Agilent BootPService
110. module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 258 Then try a firmware update see Replacing Module Firmware on page 196 If this does not help a main board replacement is required Agilent 1290 Infinity Binary Pump User Manual Troubleshooting and Diagnostics 6 Available Tests vs User Interfaces Depending on the user interface the available tests and the screens reports may vary see chapter Test Functions and Calibrations e Preferred tool should be the Agilent Lab Advisor software see Agilent Lab Advisor Software on page 90 e The Agilent ChemStation B 04 02 and above may not include any maintenance test functions e Screenshots used within these procedures are based on the Agilent Lab Advisor software Agilent 1290 Infinity Binary Pump User Manual 89 Troubleshooting and Diagnostics Agilent Lab Advisor Software 90 The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all co
111. mple passes through the detector it is necessary to correct for density related flow inaccuracies in the pump module In order to always deliver the best possible pressure stability the pump constantly tunes the delivery cycle for elasticity and dead volume effects With this feature the pump is able to deliver a stable and accurate flow without requiring individual calibration settings A further feature of the control and compensation algorithm is leak correction With this it is even possible to compensate for minor leaks in primary pump chamber inlet valve and seal without the performance of the pump being affected To increase the robustness of the pump it uses a fast defill function which reduces the delivery time of the primary piston thereby reducing the net effects of leaks considerably Furthermore each pump channel has only two valves on its two pump heads which also reduce the potential of failures In order to reduce stresses on the pump hardware it uses a smooth motion control which slowly increases or decreases the speed of the pistons to prevent shock movements In order to be able to control these movements a high resolution encoder unit is attached to the pump drives which resolves a revolution into 65000 steps and each step translates to a volume of about 300 pl Agilent 1290 Infinity Binary Pump User Manual Introduction System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for sa
112. n External Contacts board is installed The level of solvent in the bottle is denoted by the green area when the solvent level falls below the specified volume the area turns yellow when the bottle is empty the area turns red Clicking on the solvent bottle displays the Bottle Fillings dialog box The tooltip for the bottle shows the solvent name Go gt 0 oe Indicates that the ISET option is installed but with no active method gray or installed and active orange G4220A only i e Indicates the presence of a solvent selection valve Click the graphic to ke switch the valve the animation shows when the valve is switched bar The pressure setpoints The red line shows the current maximum pressure limit the green area shows the current pressure also shown as text The current solvent flow rate in mL min is displayed above the pressure display Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Instrument Signals The following pump signals are displayed Flow The current solvent flow rate in mL min Pressure The current pump ressure in bar psi or MPa see Instrument Configuration on page 54 Pressure Limit The current maximum pressure limit Composition A B The current solvent composition When a solvent selection valve is fitted the channels are shown in the graphic Context Menu The context menu of the dashboard panel contains the following commands Control Dis
113. n order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1290 Infinity Binary Pump User Manual 93 7 Error Information General Error Messages 94 General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the module as requested Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a period longer than the timeout threshold source of a not ready condition Restart the analysis where required Agilent 1290 Infinity Binary
114. n the degassing unit and the primary pump head inlet Remove the capillary connection at the top of the secondary pump head to the pump valve If installed remove the tubings of the seal wash option DO NOT REMOVE the capillary connection between the pump heads marked by the red X Agilent 1290 Infinity Binary Pump User Manual 4 Open the 4 screws holding the pump heads NOTE Open all screws step by step not screw by screw 159 9 Maintenance Replacing the Pump Heads 5 Remove the complete pump head assembly by holding it CAUTION with both hands and pulling it to the front CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 6 Install the pump head assembly by fixing the 4 screws using a 4 mm hex key and a torque wrench which are included to the 1290 Infinity Service Kit p n 5067 4699 set to 5 Nm Install screws step by step not screw by screw NOTE Do not further disassemble the pump head 160 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Pump Heads 7 Insert the heat exchanger capillary into the outlet of the primary pump head Using a torque key set 3 Nm and close the hex screw at the top
115. nally available with a seal wash function This option is recommended when using buffers or other non volatile solvents or additives that could deposit on pistons and seals It is used for regularly cleaning these parts automatically 1 Place a wash solvent reservoir into the solvent cabinet A mixture of distilled water and isopropanol 90 10 is a good choice for many applications 2 Put the solvent inlet tube into the solvent reservoir close it and connect the tube to the seal wash pump 3 Route the outlet of the wash tube into a waste container Agilent 1290 Infinity Binary Pump User Manual 47 3 Installing the Module Installation of Seal Wash Option 48 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 4 Using the Pump Leak and Waste Handling 50 Preparing the Binary Pump 51 Algae Growth in HPLC Systems 52 How to Prevent and or Reduce the Algae Problem 53 Setting up the Pump with the Instrument Control Interface 54 Overview 54 Instrument Configuration 54 The Pump User Interface Dashboard Panel 56 Control Settings 58 Method Parameter Settings 60 Priming the Pump 64 Solvent Information 66 Normal Phase Applications 74 This chapter explains the operational parameters of the Agilent 1290 Infinity Binary Pump Ee Agilent Technologies 49 4 Using the Pump Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The ha
116. nch 9 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the 4 screws at the rear of the alignment tool Tighten screws crosswise KN This procedure will align pump head parts to their correct positions and close the pump head tightly 10 Open the 3 screws which have closed the pump head alignment tool and take out the aligned pump head In case the pump head sticks inside the alignment tool you can use the handle and insert it to the rear of the tool for pushing out the pump head Heat exchanger capillary ool han GO i 182 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 11 For the primary pump head install the inlet valve and outlet valve using the torque wrench which is included to the 1290 Infinity Service Kit p n 5067 4699 Set 10 Nm for the inlet valve and 10 Nm for the outlet valve 12 Replace the filter frit and seal cap as desired Please note the correct orientation of the filter frit Hole Filter frit Seal cap Agilent 1290 Infinity Binary Pump User Manual 183 9 Maintenance CAUTION 14 Remove the alignment piston Damage to the pump head f Using a wrong torque will da
117. nd Gasket 174 Assembling the Pump Head 176 Replacing the Purge Valve Head 188 Replacing Parts of the High Pressure Filter Assembly 191 Installing the Valve Rail Kit 193 Replacing the Main Power Fuses 194 Replacing Module Firmware 196 Preparing the Pump Module for Transport 197 10 Parts and Materials for Maintenance 201 Overview of Maintenance Parts 202 Flow Connections 204 Solvent Cabinet Kit 206 Seal Wash Option 207 Pump Head Assemblies 208 Pump Head Assembly Parts 210 Primary Pump Head Parts 212 Secondary Pump Head Parts 216 Purge Valve 220 Cover Parts 222 Leak Parts 224 Fuses 224 Accessory Kit 225 Tools 226 HPLC System Tool Kit 228 Agilent 1290 Infinity Binary Pump User Manual Contents 11 Identifying Cables 229 Cable Overview 230 Analog cables 232 Remote Cables 234 BCD Cables 237 CAN LAN Cable 239 RS 232 Cable Kit 240 Agilent 1200 Module to Printer 241 12 Hardware Information 243 Firmware Description 244 Electrical Connections 247 Interfaces 249 Setting the 8 bit Configuration Switch 256 Instrument Layout 259 Early Maintenance Feedback 260 13 LAN Configuration 261 What You Have to Do First 262 TCP IP parameter configuration 264 Configuration Switch 265 Initialization mode selection 266 Dynamic Host Configuration Protocol DHCP 270 Link configuration selection 273 Automatic configuration with Bootp 274 Manual Configuration 284 PC and User Interface Software Setup Setup 290
118. ndling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F 50 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series S
119. ne end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1290 Infinity Binary Pump User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL a 9 1 White Digital ground NC 2 Brown Prepare run Low 80 3 3 Gray Start Low S NC 4 Blue Shut down Low 0 5 NC 5 Pink Not 1e connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900
120. nection between the both pump heads of one pump head assembly solvent channel can damage the connection and cause leaks gt Do not disconnect the flow connection between the pump heads Damage of internal parts gt Do not apply a strong force to the screws of the pump head gt Use a torque hex key for that purpose Agilent 1290 Infinity Binary Pump User Manual 157 9 Maintenance Verh Damage of the pump piston Removing pump heads in a position other than the maintenance position can damage the pump piston gt Before switching off the pump bring it to the maintenance position CAUTION Damage of pump drives The pump drive can be damaged if the pump initializes after switching it on without having the pump head installed properly gt Use the Lab Advisor maintenance procedure for replacing pump heads gt Install the pump head correctly before switching on the pump This procedure describes the replacement of the left pump head assembly Similarly the right pump head assembly can be replaced One pump head assembly consists of two pump heads which are both removed at the same time 158 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Pump Heads 1 In Lab Advisor go to Tools gt Remove Install Pump Head and follow instructions given on the screen 2 Close the shut off valve of the respective pump channel Y L Yt 3 Remove the flow connection betwee
121. nnected analytical instruments with instrument status Early Maintenance Feedback counters EMF instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon request the user can send this report to Agilent for a significantly improved troubleshooting and repair process The Agilent Lab Advisor software is available in two versions e Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from
122. nt selection valve Parts required p n Description 1 G4280 60029 Solvent selection valve 4 G4220 60035 Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit 1 Close shut off valve Remove tubing connections 2 Push down the SSV panel for removing it between the SSV and the solvent shut off valves and the SSV and the degassing unit inlets Agilent 1290 Infinity Binary Pump User Manual 147 9 Maintenance Replacing the Solvent Selection Valve SSV 3 Remove the connector by pushing up the small clip at the bottom of the connector 4 Install a new SSV by inserting the connector and clipping the SSV panel to the module top panel Then re install all tubing connections open shut off valve and purge valve Al B1 A e Hoe B TE 148 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Changing configuration or replacing the Jet Weaver When For optimizing the pump configuration to mixing performance or low delay volumes fast gradients see chapter Optimizing Performance Tools required p n Description 8710 0510 inch wrench 3 mm hex key Parts required p n Description 1 G4220 60006 Jet Weaver 35 pL 100 pL 1 G4220 60012 Jet Weaver 380 uL OPTIONAL 1 G4220 87000 Capillary ST 0 17 mm x 300 mm Valve to Jet Weaver Agilent 1290 Infinity Binary Pump User Manual 149 9 Maintenance 1 Remove capillary connecti
123. ntalum SST gold ruby ZrO based ceramic tantalum SST Zr04 based ceramic UHMW PE SST SST Polyimide SST DLC 1 to THF Ultra clean tubings are available for the use with high end MS detectors They are also compatible 2 Jet Weaver Heat Exchanger Agilent 1290 Infinity Binary Pump User Manual 67 4 68 Using the Pump Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperatur
124. of the outlet water Install flow connections Connect the degassing unit outlet to the inlet of the primary pump head and the outlet of the secondary pump head to the inlet of the purge valve Channel A left pump head assembly is connected to port 4 channel B right pump head assembly to port 1 9 After the complete reassembly of the module the pump must be purged For 10 min apply a flow of 10 ml min using solvents suitable for your application and a composition 50 50 for channels A and B for example 50 acetonitrile 50 Agilent 1290 Infinity Binary Pump User Manual 161 9 Maintenance Disassembling the Pump Head When p n Description G4220 20012 G4220 20013 G4220 20014 G4220 20015 8710 0510 Tools required 4 mm hex bit Preparations 1 4 2 5 mm Hex Bit Adapter in square to hex Wrench open 1 4 5 16 inch If parts inside the pump head need to be replaced Torque wrench 2 25 Nm Remove the pump head assembly as described in Replacing the Pump Heads on page 156 steps This procedure describes replacements for the pump heads of channel A Replacement for channel B can be done accordingly The primary pump head does not have a heat exchanger Seal wash parts are optional for both pump heads 1 Open the 2 5 mm hex screw at the top of the primary pump head which fixes the connection capillary of the heat exchanger Then lift up the capillary and remove it from the
125. og detector signal tional optional EN i PA CAN Bus cable Thermo cable optional AC Power Figure 6 Recommended two stack configuration for 1290 Infinity rear view 34 Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copi
126. olvent Cabinets For details on correct installation see section Installation Information on Leak and Waste Handling in the service manual Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Preparing the Binary Pump For best performance of the pump e Place solvent cabinet with the solvent bottles always on top or at a higher level of the pump e For optimum performance use the built in degasser This is mandatory for flow rates below 0 5 mL min and for configurations without Jet Weaver When using the pump with vacuum degassing unit flush the degassing unit with at least 5 mL per channel before operating the pump especially when the pumping system had been turned off for a certain length of time for example overnight and volatile solvent mixtures are used in the channels e Prevent blocking of solvent inlet filters never use the pump without solvent inlet filters Growth of algae should be avoided see Algae Growth in HPLC Systems on page 52 e Check pump outlet filters and column frit in regular time intervals A blocked pump outlet filter can be identified by black yellow or greenish layers on its surface Whenever possible use a minimum flow rate of 5 uL min per solvent channel to avoid crossflow of solvent into the unused pump channel e When using buffer solutions flush the system with water before switching it off The optional seal wash function should be used when buffer solutions w
127. on Quantity 1290 Infinity Bin Pump User Manual 1 1290 Infinity System Manual 1 Agilent Lab Advisor 1 User Manual on Documentation CD part of the shipment not module 1 per order specific Power Cord 1 Solvent Cabinet Kit with 4 bottles 1 Accessory Kit see Accessory Kit on page 225 1 RRHD Eclipse Plus C18 2 1x50 mm 1 8 u 1 Agilent 1290 Infinity Binary Pump User Manual 29 3 Installing the Module Optimizing the Stack Configuration 30 If your module is part of a complete Agilent 1290 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume For other possible configurations please refer to the Agilent 1290 Infinity System Manual One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Binary LC System in the following configuration See Figure 3 on page 31 and Figure 4 on page 32 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required The Agilent 1290 Infinity Binary Pump should always be installed at the bottom of the stack Agilent 1290 Infinity Binary Pump User Manual Installing the Module Optimizing the Stack Configuration HN WN AUN NAAT Instant Pilot Solvent cabinet Detector Column compartment Autosampler Figure 3 Recommended
128. on because the solute is retained longer In gradient separations the retention is described by k in the following equation tc F100 A B V S m where k mean k value tg time length of gradient or segment of gradient min F flow ml min Vi column delay volume e A B change in fraction of solvent B during the gradient e S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constant and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can generate higher peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008 Maximizing peak capacity and separation speed in liquid chromatography Agilent 1290 Infinity Binary Pump User Manual 83 5 Optimizing Performance Using Solvent Calibration Tables 84 Importing Solvent Calibration Tables RC NET based Agilent graphical user interfaces ChemStation EZChrom Elite OpenLab etc include data for most commonly used solvents in HPLC This data contains solvent properties and is used fo
129. on of the hardware configuration The system configures the instrument automatically with no further manual configuration necessary The Binary Pump configuration parameters are in two sections Communication Options Communication The parameters in this dialog box are detected automatically during autoconfiguration Device name Type ID Serial number Firmware revision Button Connection settings Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Options Pressure Unit select the pressure units from the drop down list bar psi or MPa Seal wash installed This check box is marked to indicate that an optional seal wash has been detected during autoconfiguration ISET installed This check box is marked to indicate that ISET is installed Click ISET Configurations to open the ISET Configuration dialog box which allows you to configure a sampler for the ISET emulation Configure Solvent Type Catalogs Displays the Solvent Type Catalogs dialog box which allows you to import and export solvent calibration data See Importing Solvent Calibration Tables on page 84 Please refer to the online help of your user interface for more detailed information Agilent 1290 Infinity Binary Pump User Manual 55 4 56 Using the Pump The Pump User Interface Dashboard Panel Module Graphic The items in the pump graphic have the following meaning and function Indicates that a
130. on to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1290 Infinity Binary Pump User Manual In This Guide In This Guide This manual covers the Agilent 1290 Infinity Binary Pump G4220A 1 Introduction This chapter gives an introduction to the pump instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Module This chapter gives information about the preferred stack setup for your system and the installation of your Agilent 1290 Infinity Binary Pump 4 Using the Pump This chapter explains the operational parameters of the Agilent 1290 Infinity Binary Pump 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1290 Infinity Binary Pump User Manual 3 In This Guide 8 Test Functions and Calibrations This chapter
131. ons from the Jet Weaver 2 Remove the hex screws that fix the Jet Weaver to the pump housing The standard Jet Weaver Jet Weaver 35 uL 100 uL G4220 60006 has a front and a rear side with different internal volumes 35 100 uL that are optimized for a low delay volume or best mixing performance Please refer to recommendations in the Agilent 1290 Infinity System Manual The optional Jet Weaver Jet Weaver 380 pL G4220 60012 is recommended for applications which are challenging with respect to mixing noise e g TFA applications and has just one side 150 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Changing configuration or replacing the Jet Weaver 3 Install new Jet Weaver or flip the Jet Weaver for 4 Reinstall the capillary connections backside The inlet at the bottom of the Jet Weaver is connected to the central port of the pump valve by a capillary length 300 mm 0 17 mm i d The outlet at the top is connected to the autosampler Agilent 1290 Infinity Binary Pump User Manual 151 9 Maintenance Replacing the Seal Wash Pump When In case of wear of the seal wash pump Parts required p n Description 5042 8507 Peristaltic Pump for Seal Wash Preparations Remove the shutoff valve panel Replacing the Shutoff Valve Panel on page 136 and flow connections from and to the seal wash pump 1 For removing the seal wash pump press
132. oper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Agilent 1290 Infinity Binary Pump User Manual 295 14 Appendix Operation 296 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy
133. organic solvents Use a minimum flow rate of 5 uL min or 1 composition per solvent channel whatever is greater to avoid cross flow Cross flow can be caused by micro leaks in pump heads and can result in buffer precipitation in pump heads channel blocking or reduced pump head life time through wear of seals and pistons Consider using an inline filter for example Inline filter G1311 60006 Never leave buffers in a system without flow Before shutting down a system flush it extensively with warm water to avoid clogging of valves capillaries or flow cells or reducing the life time of your column If the system is not used for some time for example more than a day depending on lab temperature fill all solvent lines with organic solvent or water with at least 10 isopropanol Regularly maintain the LC system Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Handling of Acetonitrile Acetonitrile is a solvent that is frequently used in reversed phase chromatography Despite of its common use it can be a source of issues if not handled correctly Acetonitrile degrades through polymerization and such polymers can stick to surfaces in LC systems and e g cause issues with valve performance and therefore affect retention time precision Polymers can also show up as background noise in MS detectors When using acetonitrile e Use high quality solvents from renowned suppliers e Use fresh solvents and filter them
134. ork device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 22 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex Agilent 1290 Infinity Binary Pump User Manual 273 13 LAN Configuration Automatic configuration with Bootp All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the detector configuration switch is set properly The setting should be either BootP or BootP amp Store see Table 18 on page 266 Assure that the detector connected to the network is powered off If the Agilent BootP Service program is not already installed on your PC then install it fro
135. ort Foam 39 Installing the Pump 40 Flow connections tothe pump 43 Installation of Seal Wash Option 47 This chapter gives information about the preferred stack setup for your system and the installation of your Agilent 1290 Infinity Binary Pump ete Agilent Technologies 27 3 Installing the Module Unpacking the Module Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 28 Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 Delivery Checklist Ensure that all parts and materials have been delivered with your module The delivery checklist is shown below For parts identification please check the illustrated part lists in Parts and Materials for Maintenance on page 201 Please report any missing or damaged parts to your local Agilent Technologies sales and service office Table 3 Binary Pump Checklist Descripti
136. our Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Agilent 1290 Infinity Binary Pump User Manual 97 7 Error Information Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative Please contact your Agilent service representative 2 Defective leak sensor 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 98 1 Loose conne
137. outlet of the secondary pump head 2 Usea 14 mm hex wrench for opening the filter assembly of the secondary pump head 3 Replace the filter frit and seal cap as desired Please note the correct orientation of the filter frit Hole Filter frit Seal cap 4 Re install the filter assembly using the torque wrench 14 mm hex bit set to 16 Nm 192 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Installing the Valve Rail Kit When Tools required Parts required This rail is needed for the installation of external valves Description Pozidrive screwdriver 1 p n Description 1 5067 4634 Valve Rail Kit The rail can be installed on the left or right side of the pump This procedure describes the installation on the left side and applies similarly to the right side 1 The valve rail is fixed to the pump cover by 4 screws The position of the lower screws is marked on the module cover First tighten these screws and then tighten the upper screws Agilent 1290 Infinity Binary Pump User Manual 193 9 Maintenance Replacing the Main Power Fuses When Tools required Parts required Preparations If the main power LED is off while the main power button is pressed see Status indicators on page 87 Description Flat head screwdriver p n Description 1 2110 1004 Fuse 10At Switch off the instrument and unplug the main power cable Fire hazard Using wrong
138. ower limit 103 pressure exceeded upper pressure imit 102 pressure sensor calibration wrong or missing 117 pump drive blocked or encoder failed 107 pump drive encoder defect 110 pump drive encoder error 115 pump drive encoder rollover 111 pump drive error 115 pump drive stop not found 116 pump drive stroke blocked 116 300 pump error 102 pump head leak test 126 purge valve failed 111 reading of purge valve tag failed 111 reading the pump encoder tag failed 106 remote timeout 96 seal wash pump was missing when tried to turnon 117 shutdown 95 solvent counter exceeded limit 104 system pressure test 123 target pressure not reached for binary pump degasser 103 timeout 94 unknown purge valve type 114 waste counter limit exceeded 105 writing the pump encoder tag failed 107 writing the purge valve tag failed 113 extra column volume 78 F fan failed 99 filter solvent inlet 51 firmware description 244 main system 244 resident system 244 update tool 245 updates 245 196 upgrade downgrade 196 fittings 135 flow connections topump 43 flow rate limit exceeded 105 flow rate minimum 51 frequency range 23 G general error messages 94 handling acetonitrile 73 handling acids 73 handling buffers 72 heat exchanger replace 171 humidity 23 initialization mode selection 266 installation bench space 22 power considerations 20 seal wash option 47 site requirements 19 instrument layout 259
139. pening in the pump head and lift it over the pins Spacer fitting 6 Use the 19 mm screw for fixing the front plate Front plate 7 Insert and fix the screw Screw Agilent 1290 Infinity Binary Pump User Manual 173 9 Maintenance Replacing Wash Seal and Gasket Tools required p n Description 01018 23702 Insert tool Parts required p n Description 0905 1718 Wash Seal PE 5062 2484 Gasket seal wash pack of 6 1 Use the steel side of the insert tool for removing the 2 Use the soft plastic side of the insert tool for inserting the wash seal wash seal into the support ring Wet the wash seal with isopropanol or another suitable solvent before insertion amp Insert tool Wash seal Wash seal SS EE y Support ma Sy N AN 174 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 3 Put the gasket into the support ring and insert the seal holder Seal holder with backup ring Gasket Support ring Agilent 1290 Infinity Binary Pump User Manual 175 9 Maintenance Assembling the Pump Head When Before installing the pump head Tools required p n G4220 20012 64220 20013 G4220 20041 G4220 20015 01018 23702 Parts required p n 0905 1420 Description Pump head alignment tool Torque wrench 2 25 Nm 4 mm hex bit Bit Torx 10x25 mm Adapter in square to hex Insert tool Description PE seals pack of 2 See chapter
140. plays the pump s Control dialog box Method Displays the pump s Method Setup dialog box Set Error Method Sets the method that is loaded if an error occurs to the method that is currently available in the hardware Identify Device Causes the LED on the front of the module to blink for a few seconds B Switch Pump On Off Toggles the status of the pump on or off lt i Bottle Fillings Displays the Bottle Fillings dialog box Purge On Off Allows you to control the purging of the system Prime On Off Allows you to prime the pump heads for initially drawing solvent Conditioning On Off Allows you to switch pump conditioning on and off The conditioning function is useful for removing small air bubbles inside the pump flow path Agilent 1290 Infinity Binary Pump User Manual 57 4 Using the Pump Control Settings The Binary Pump control parameters are in six sections Seal Wash e Automatic Turn On Pump e Purge e Prime e Conditioning Table4 Pump control parameters Parameter Limits Description Pump Enables you to switch the pump On Off or to a Standby condition In the Standby condition the pump motor is still active and when the pump is switched on again does not need to be re initialized Seal Wash The seal wash can be set up to be run once Single wash or periodically Seal Wash Run Mode Automatic Turn On Periodic Off no seal wash is used Single wash the seal will be purged for a specifi
141. presentative Current of primary pump drive too high Error ID 22433 The current of the primary pump drive is too high e Parameter 1 or 4 referring to pump drive Probable cause Suggested actions 1 Blockage of flow path between primary Check for blockages in flow path pump head and pressure sensor e g of the e Please contact your Agilent service heat exchanger representative 2 Primary pump drive is defect Please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual 113 7 Error Information Current of secondary pump drive too high Error ID 22434 The current of the secondary pump drive is too high Parameter 2 or 3 referring to pump drive Probable cause Suggested actions 1 Blockage of flow path between secondary Check for blockages in the flow path pump head and pressure sensor e g ofthe Please contact your Agilent service heat exchanger representative 2 Secondary pump drive is defect Please contact your Agilent service representative Unknown purge valve type Error ID 22435 The type information of the purge valve is invalid e Parameter None Probable cause Suggested actions 1 Wrong valve head installed Check or replace purge valve head 2 Valve head has invalid RFID tag content Check or replace purge valve head 114 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Pump drive encoder error Error ID 22437 The pump dri
142. r Agilent service representative Pump drive encoder rollover Error ID 22424 Invalid pump drive encoder signals have been detected e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual 111 7 Error Information Drive position limit Error ID 22425 Internal error Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Internal error Please contact your Agilent service representative Insufficient power of drive encoder LED Error ID 22426 Insufficient power of drive encoder LED Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Drive encoder error Error ID 22427 22430 An error has occurred for the pump drive encoder Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative 112 Agilent 1290 Infinity Binary Pump User Manual Error Information 7 Writing the purge valve tag failed Error ID 22431 Writing the purge valve tag failed e Parameter None Probable cause Suggested actions 1 Purge valve head tag defect Replace purge valve head 2 Purge valve tag reader is defect Please contact your Agilent service re
143. r optimum pump control in order to ensure best flow and composition accuracy If your solvent is not included to the software please check the Agilent web site http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 for additional libraries registration required which also provides updates and optimized data If your solvent is neither available in the user interface nor in the library please use generic solvents Generic aqueous gives good results for most solvent mixtures with at least 50 water which have similar properties as pure water For other solvents with high organic percentage Generic organic gives a good approximation Importing Solvent Calibration in ChemStation 1 Go to menu Instrument gt Instrument configuration 2 In the Instrument Configuration screen choose your module and click Configure Click Configure Solvent Type Catalogs In Solvent Type Catalogs click Import Navigate to the location of the solvent calibration table and click Open The new solvent will now appear in the Solvent Type Catalogs y Oo CFF F Q The imported solvent is now available for selection as a solvent type see Table 5 on page 60 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 86 Status indicators 87 Power Supply Indicator 87 Module Status Indicator 88 Availa
144. r position lt 70 dB e Sound Pressure Lp lt 70 dB A At Operator Position Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 298 Agilent 1290 Infinity Binary Pump User Manual Index 8 bit configuration switch on board LAN 256 A Agilent Lab Advisor software 90 Agilent Lab Advisor 90 Agilent oninternet 298 User Interface Software Setup 290 algae 51 ambient non operating temperature 23 ambient operating temperature 23 analog signal 253 analog cable 232 apgremote 254 automatic configuration with Bootp 274 BCD cable 237 bench space 22 binary pump shutdown during analysis 106 BootP service installation 276 restart 283 settings 283 stop 282 Bootp amp Store 267 automatic configuration 274 initialization modes 266 using default 268 using stored 268 buffer application 51 C cable analog 232 BCD 237 CAN 239 LAN 239 remote 234 RS 232 240 cables analog 230 BCD 230 CAN 231 LAN 231 overview 230 remote 230 RS 232 231 CAN cable 239 capillaries 135 cleaning 134 compensation sensor open 98 compensation sensor short 99 condensation 22 configuration switches 265 configuration low flow rates 51 one stack 30 two stack front 33 two stack rear 34 two stack 33 Agilent 1290 Infinity Binary Pump User Manual Inde
145. ransfer and synchronization e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1290 Infinity Binary Pump User Manual 247 12 Hardware Information Electrical Connections Rear View of the Module APG Remote AN Bus b R L ce ae C JC gt by
146. re and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attenti
147. required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 13 LAN Configuration What You Have to Do First 262 TCP IP parameter configuration 264 Configuration Switch 265 Initialization mode selection 266 Dynamic Host Configuration Protocol DHCP 270 General Information DHCP 270 Setup DHCP 271 Link configuration selection 273 Automatic configuration with Bootp 274 About Agilent BootP Service 274 How BootP Service Works 275 Situation Cannot Establish LAN Commun
148. rfaces 255 special settings boot resident 258 forced cold start 258 specification physical 23 specifications 19 performance 24 status indicator 88 System pressure test errormessage 123 system setup and installation optimizing stack configuration 30 system tool kit 228 T target pressure not reached for binary pump degasser 103 TCP IP parameter configuration 264 telnet 302 configuration 285 temperature sensor 100 test functions 86 tests 120 timeout 94 troubleshooting error messages 86 93 status indicators 86 87 U unknown purge valve type 114 unpacking 28 user interfaces 89 V vacuum degassing unit 51 voltage range 23 WwW waste counter limit exceeded 105 weight 23 writing the pump encoder tag failed 107 writing the purge valve tag failed 113 Agilent 1290 Infinity Binary Pump User Manual www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity Binary Pump G4220A introduction and specifications e installation e using and optimizing troubleshooting and diagnose e maintenance e parts identification hardware information e safety and related information Agilent Technologies 2010 2014 2015 Printed in Germany 02 2015 G4220 90006 Rev E fe Agilent Technologies
149. ription Screw M5 40 mm long Pump Head Screw Pump Head Front Plate order pump head Heat Exchanger secondary pump head only Channel A Heat Exchanger secondary pump head only Channel B Headless screw for 1290 Infinity pump heads Spacer Fitting Pump Chamber Housing order pump head PE Seal Seal holder including backup ring Gasket seal wash pack of 6 Wash Seal PE Support Ring Seal Wash Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic High Pressure Filter Assembly secondary pump head PTFE frits pack of 5 Seal cap 217 10 Parts and Materials for Maintenance Secondary Pump Head Without Seal Wash Binary Pump Figure 30 Secondary pump head Binary Pump without seal wash 218 Agilent 1290 Infinity Binary Pump User Manual Item 10 11 12 13 14 15 Agilent 1290 Infinity Binary Pump User Manual p n 0515 1218 G4220 20003 G4220 81013 G4220 81012 64220 20028 64220 20001 0905 1719 4220 60015 2x 5067 5678 64280 60026 01018 22707 5067 4728 Parts and Materials for Maintenance 10 Description Screw M5 40 mm long Pump Head Screw Pump Head Front Plate order pump head Heat Exchanger secondary pump head only Channel A Heat Exchanger secondary pump head only Channel B Headless screw for 1290 Infinity pump heads Spacer Fitting Pump Chamber Housing order pump head PE Seal Support ring including bac
150. rque wrench 18 Install the pump head assembly by fixing the 4 screws using a 4 mm hex key and a torque wrench which are included to the 1290 Infinity Service Kit p n 5067 4699 set to 5 Nm Install screws step by step not screw by screw 186 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Assembling the Pump Head 19 Insert the heat exchanger capillary into the outlet of the 20 Install flow connections primary pump head Using a torque key set 3 Nm and Connect the degassing unit outlet to the inlet of the close the hex screw at the top of the outlet primary pump head and the outlet of the secondary pump head to the inlet of the purge valve Channel A left pump head assembly is connected to port 4 channel B right pump head assembly to port Agilent 1290 Infinity Binary Pump User Manual 187 9 Maintenance Replacing the Purge Valve Head When Parts required Preparations CAUTION In case of problems with the purge valve p n Description 1 5067 4119 Purge valve head 2 5067 4655 Capillary ST 0 25 mm x 235 mm to pump head assemblies channel A and B 2x 1 G4220 87000 Capillary ST 0 17 mm x 300 mm Valve to Jet Weaver 2 5067 4656 Capillary ST 0 25 mm x 80 mm to pressure sensor 2x Remove all capillary connections to the purge valve Potential damage of valve head or malfunction of valve When the pump is switched on the
151. ry of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does not offer any services for hostname resolution e g NetBIOS DHCP Active Server Parameter Figure 49 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 Setup DHCP Software required The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section System Info Controller DE12345678 G4208A Main Revision B 02 12 0001 DAD DE64260019 G1315D Main Revision B 06 41 0002 a Resid
152. s effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection The delay volume in a system includes the volume in the pump from the point of mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column 78 Agilent 1290 Infinity Binary Pump User Manual Optimizing Performance 5 How to Configure the Optimum Delay Volume The physical delay volume of the pump depends primarily on the use of the Jet Weaver mixer For UV detection the Jet Weaver should always be used but for mass spectrometric detection the user can decide to bypass the Jet Weaver in order to reduce the delay volume This only makes sense for ultra fast gradient operation less than 0 5 min or for use with very small volume columns If the Jet Weaver is bypassed the connection tubing to the autosampler is routed directly from the purge valve Before disconnecting a Jet Weaver from the flow path flush it with organic solvent Avoid leaving water or buffers inside the Jet Weaver which may cause the growth of microorganisms like algae or bacteria Sometimes it may be advisable to increase the delay volume in the pump Spec
153. s very uncommon for normal phase applications no buffers needed but wash seals are needed for seal wash pump heads Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 1290 Infinity pumps use PE seals by default In combination with ceramic pistons PE seals are used for both reversed phase 1200 bar and normal phase applications 1260 Infinity pumps use sapphire pistons and black PTFE piston and wash seals by default 600 bar Such PTFE seals create small wear particles in normal phase applications which can clog valves and other parts in the flow path PE seals have a limited life time when used with normal phase solvents and sapphire pistons Agilent recommends a maximum pressure of 200 bar for this combination which shall also be applied for pressure tests Tetrahydrofuran THF is not compatible with PE seals Black PTFE seals should be used with THF even in 1290 Infinity pumps In this case use a maximum pressure of about 400 bar Solvent libraries for G4220A B are available at http www chem agilent com _layouts agilent downloadFirmware aspx whid 62265 Choice of Normal Phase Valves and Seals Table 8 Recommended valves and seals for normal phase applications 1260 Infinity 1290 Infinity Inlet valves 1260 Infinity Inlet Valve Type N 1290 Infinity Inlet Valve Type N G1312 60166 G4220 60122 Outlet valves 1260 Infinity Outlet Valve Type 1290 Infinity Outlet Valve Type N SFC G1312 60167 N G422
154. scription LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Preparing the Pump Module for Transport When If the module shall be transported or shipped Parts required p n Description 1 9301 0411 Syringe Plastic 1 9301 1337 Syringe adapter 1 G4220 44000 Protective Foam Preparations Flush both solvent channels with isopropanol CAUTION Mechanical damage gt For shipping the module insert the Protective Foam to protected the module from mechanical damage gt Be careful not to damage tubing or capillary connections while inserting the module in the Protective Foam Agilent 1290 Infinity Binary Pump User Manual 197 9 Maintenance Preparing the Pump Module for Transport 1 Remove solvent inlets from solvent res
155. ses and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Agilent 1290 Infinity Binary Pump User Manual Using the Pump 4 Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide ZrO Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it
156. ss it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored Agilent 1290 Infinity Binary Pump User Manual 269 13 LAN Configuration Dynamic Host Configuration Protocol DHCP 270 General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memo
157. ssembling the pump head remove the 4 hex screws at the rear of the pump head Agilent 1290 Infinity Binary Pump User Manual 167 Maintenance 3 Remove the front part of the pump head including pump chamber housing with pump seal and seal holder Remove the support ring with wash seal and gasket Support ring with wash seal Seal wash gasket Seal holder including backup ring Pump chamber ousing 4 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 5 Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals 6 Use the steel side of the insert tool for removing the pump seal from the pump chamber housing Insert tool Pump seal Do not use the pump piston for that purpose as this can break it 168 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 7 To flush out particles from the pump head use 8 To remove wear use isopropanol pressurized air 1 2 and 3 9 Toclean the piston and the pump head sufrace use the ultra fine abrasive mesh p n 8660 0852 Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection Agilent 1290 Infinity Binary Pump User Manual 169 9 Maint
158. ssory kit to the original shipment box 200 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 10 Parts and Materials for Maintenance Overview of Maintenance Parts 202 Flow Connections 204 Solvent Cabinet Kit 206 Seal Wash Option 207 Pump Head Assemblies 208 Pump Head Assembly Parts 210 Primary Pump Head Parts 212 Secondary Pump Head Parts 216 Purge Valve 220 Cover Parts 222 Leak Parts 224 Fuses 224 Accessory Kit 225 Tools 226 HPLC System Tool Kit 228 This chapter provides information on parts for maintenance ee Agilent Technologies 201 10 Parts and Materials for Maintenance Overview of Maintenance Parts Overview of Maintenance Parts Figure 22 Overview of main assemblies 202 Agilent 1290 Infinity Binary Pump User Manual Parts and Materials for Maintenance 10 Item p n Description 1 G4220 60006 Jet Weaver 35 pL 100 pL G4220 60012 Jet Weaver 380 pL OPTIONAL 2 G4280 60029 Solvent selection valve 3 5042 8507 Peristaltic Pump for Seal Wash OPTIONAL 4 G4220 60001 Pressure sensor 1200 bar 5 5067 4119 Purge valve head 6 n n Pump Head Assemblies for details on pump head assemblies refer to Pump Head Assemblies on page 208 Agilent 1290 Infinity Binary Pump User Manual 203 10 Parts and Materials for Maintenance Flow Connections Flow Connections 204 MK G I wy S O KN Bi AOKO M SOE D PERATA
159. stack configuration for 1290 Infinity front view Agilent 1290 Infinity Binary Pump User Manual 31 3 Installing the Module Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Instant Pilot Analog detector signal optional AC Power CAN Bus cable Figure 4 Recommended stack configuration 1290 Infinity rear view 32 Agilent 1290 Infinity Binary Pump User Manual Installing the Module 3 Optimizing the Stack Configuration Two Stack Configuration In case the autosampler thermostat is added to the system a two stack configuration is recommended which places both heavy modules 1290 Infinity pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 5 on page 33 and Figure 6 on page 34 Instant Pilot Detector Column compartment ANIMA UUNIT A Solvent cabinet b Pump j i p e e r ee j q NU Autosampler Thermostat for the ALS optional Figure 5 Recommended two stack configuration for 1290 Infinity front view Agilent 1290 Infinity Binary Pump User Manual 33 3 Installing the Module Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Instant Pilot Anal
160. sure limit Replace column Target pressure not reached for binary pump degasser Error ID 22031 The target pressure of the binary pump degasser has not been reached within the expected time Parameter Pressure in mbar Probable cause 1 Condensation in degasser chamber due to temperature fluctuation 2 Degasser is defect Agilent 1290 Infinity Binary Pump User Manual Suggested actions Equilibrate and restart module Please contact your Agilent service representative 103 7 7 Error Information Degasser s pressure limit violation Error ID 22032 Pressure too far above the limit Probable cause Suggested actions 1 Leak in degasser chamber or degasser Please contact your Agilent service tubing representative 2 Defect vacuum pump Please contact your Agilent service representative 3 Degasser chamber empty or connected to Block unused degasser channels air Solvent counter exceeded limit Error ID 22055 The counter for the solvent volume has exceeded the limit which has been set in the user interface Parameter e Without Solvent Selection Valve 0 for channel A 1 for channel B e With Solvent Selection Valve 2 for channel Al 3 for channel B1 4 for channel A2 5 for channel B2 Probable cause Suggested actions 1 No solvent present Refill solvent bottle 2 Inappropriate setting Check solvent counter setting in user interface 104 Agilent 1290 Infinity Binary Pump User Manual Waste co
161. system a 5 00 mL min change in the mobile phase viscosity or a temperature change For each channel you can select which of the two solvents to deliver You can set the percentage of solvent B to any value from 0 through 100 Solvent A always delivers the remaining volume 100 B The solvent B check boxes allow you to turn the solvent B channels on checked or off cleared When the Use solvent types check box in the Compressibility section is checked see Advanced Settings on page 62 you click the down arrow and select either a Generic solvent or a calibrated Solvent Stoptime 0 01 99999 min or The stoptime sets a time limit for your analysis After the stoptime all AsInjector NoLimit gradients are stopped and the pump parameters return to their initial an infinite runtime values The pump can be used as a stoptime master for the complete analytical system The pump also stops the detectors if they have a No Limit stoptime setting If no limit is given a method will have to be stopped manually Posttime 0 01 99999 min or Your instrument remains in a not ready state during the posttime to delay Off 0 0 min the start of the next analysis You can use the Posttime to allow your column to equilibrate after changes in solvent composition for example after gradient elution 60 Agilent 1290 Infinity Binary Pump User Manual Table5 Method parameters Using the Pump 4 Parameter Limits Description
162. t 1290 Infinity Binary Pump User Manual Using the Pump 4 How to Prevent and or Reduce the Algae Problem e Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters e Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber 9301 1450 supplied with the instrument for your aqueous mobile phase e If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase Agilent 1290 Infinity Binary Pump User Manual 53 4 Using the Pump Setting up the Pump with the Instrument Control Interface 54 Overview Parameters described in following sections is offered by the instrument control interface and can usually be accessed through Agilent instrument control software For details please refer to manuals and online help of respective user interfaces Instrument Configuration Use the Instrument Configuration dialog box to examine and if necessary modify your instrument configuration The Configurable Modules panel contains a list of all modules available for configuration The Selected Modules panel contains the list of configured modules Auto Configuration Under Communication settings select either the Host Name option or the IP address option and enter the appropriate value for the host computer to enable automatic detecti
163. t exchanger Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench Agilent 1290 Infinity Binary Pump User Manual 177 Maintenance 2 Use the soft plastic side of the insert tool for inserting the piston seal to the pump chamber housing Wet the piston seal with isopropanol or another suitable solvent before insertion Insert tool Pump seal Pump chamber housing 178 Insert the support ring and pump head ferrules into the piston housing Observe the pins on the support ring which help you assembling the pump head correctly Pins second pin not shown ANO Pump head ferrules Support ring Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Assembling the Pump Head 3 Assemble the pump head by putting the pump chamber housing on top of the support ring Observe correct orientation of the pin Pump chamber housing Pump head ferrules Support ring Piston housing Do NOT install the inlet and outlet valves primary pump head and the high pressure filter secondary pump head at this stage 4 Loosely close the 4 screws at the rear of the pump head The screws will be fixed tightly later Agilent 1290 Infinity Binary Pump User Manual 179 9 Maintenance 5 Insert the alignment piston of the pump head
164. t session 1 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK 2 Type the following at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt E C AWINDOWS system32 cmd _exe C gt telnet 134 46 27 95 Figure 57 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switch on page 265 When the connection was established successfully the module responds with the following ET C AWINDOWS system32 cmd exe telnet 134 40 27 95 Agilent Technologies G1i315C PP H000024 Figure 58 A connection to the module is made Agilent 1290 Infinity Binary Pump User Manual 285 13 LAN Configuration 3 Type and press enter to see the available commands WINDOWS system32 cmd_exe telnet 134 40 27 95 Agilent Technologies G1315C PP 88680824 gt command syntax description display help info display current LAN settings ip lt x x x x gt set IP Address sm X X X X gt set Subnet Mask gu lt x x x x gt set Default Gateway exit exit shell gt Figure 59 Telnet Commands Table 23 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X xX gt sets new ip address sm
165. ted pump performance problems Preparations Solvents must be present in both channels 124 Agilent 1290 Infinity Binary Pump User Manual Test Functions and Calibrations 8 Pump Head Leak Test 1 Run the Pump Head Leak Test with the recommended user interface for further information see Online Help of user interface Limits Signals Test Name Pump Head Leak Test Description No description Module G42204 LPO00000005 Approx Time 5 min Status Running CEE EEE eee Test Procedure Result Name Value Y 1 Checking pre requisites pump on etc Maximum system pressure 1200 bar Y 2 Enter the test pressure Y 3 Configure purge process and parameter u 4 System checking leak tightness in channel 5 System checking leak tightness in channel B 6 Evaluate results 7 Restore system configuration Figure 16 Pump Head Leak Test Results Pump Head Leak Test Enter the test pressure Enter the pressure at which the test will be executed 1200 bar Figure 17 Pump Head Leak Test dynamic pressure input Agilent 1290 Infinity Binary Pump User Manual 125 8 Test Functions and Calibrations Pump Head Leak Test Evaluation Test Failed Probable cause Suggested actions 1 Loose or leaky fittings Tighten the fittings or replace capillaries 2 Leaky High Pressure Filter Assembly Tighten Filter housing and fitting NOTE Fittings should only be tightened in
166. th 1260 and 1290 Infinity pumps do not work well with applications using non polar solvents as for normal phase applications e g hexane and heptane With such applications pressure drops could be observed They are a result of particles electrostatically charging up in insulating solvents and sticking to the balls inside the valves such that the valves do not close properly any more after some time of use can be hours For normal phase applications a second type of valves is available which has a design based on the existing one for 1260 and 1290 Infinity valves These valves use a new material for valve balls which is a conductive ceramic and replaces non conductive ruby balls The balls do not charge up electrostatically and show good performance in normal phase The valves are marked with N for non polar or normal phase Agilent recommends using these valves for and only for normal phase applications Corrosion of valves Normal phase balls valves corrode quickly in aqueous solutions and acids at or below pH 7 gt Do not use normal phase valves in applications running with aqueous solutions 74 The N Valves have been tested successfully in using hexane at pressures below 100 bar heptane can be used as a substitute for neurotoxic hexane Seals for Normal Phase Applications For running normal phase on 1200 Infinity Series pumps yellow PE seals are required which exist as piston seals and wash seals Seal wash i
167. tion PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 254 Agilent 1290 Infinity Binary Pump User Manual Hardware Information 12 Table 13 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START
168. tion mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp gt Active Parameter Server Figure 45 Bootp Principle Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter
169. tivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an automated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting Agilent 1290 Infinity Binary Pump User Manual 81 5 82 Optimizing Performance The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give a higher plate number The pressure rises with the inverse square of the particle size and proportionally with the length of the column This is the reason that the 1290 Infinity LC system was designed to go to 1200 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4
170. tocol Intemet Protocol The default pEenered ONS You can get IP settings assigned automatically if your network supports ie ne cali Dre is De ies connie Altemate DNS this capability Otherwise you need to ask your network administrator for the appropriate IP settings Show icon in taskbar when connected Obtain an IP address automatically C Use Wetoporine scares automatic IP address via DHCP tli I requires special IT setup of the network Obtain DNS server address automatically C Use the following DNS server addresses Figure 64 Changing the TCP IP settings of the PC 290 Agilent 1290 Infinity Binary Pump User Manual LAN Configuration 13 User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide Agilent 1290 Infinity Binary Pump User Manual 291 13 LAN Configuration PC and User Interface Software Setup Setup 292 Agilent 1290 Infinity Binary Pump User Manual Agilent 1290 Infinity Binary Pump User Manual 14 Appendix General Safety Information 294 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 297 Radio Interference 298 Sound Emission 298 Agilent Technologies on Internet 298 This chapter provides addition information on safety legal and web ee Agilent Technologies 293 14 Appendix General Safety Information Safety Symbols Table 24 Safety Symbols Symbol Description
171. trument using BootP Service 1 Power cycle the Instrument 2 After the instrument completes self test open the log file of the BootP Service using Notepad The default location for the logfile is C Documents and Settings All Users Application Data Agilent BootP LogFile The logfile will not be updated if it is open The contents will be similar to the following 02 25 10 15 30 49 PM Status BootP Request received at outermost layer Status BootP Request received from hardware address 0010835675AC Error Hardware address not found in BootPTAB 0010835675AC Status BootP Request finished processing at outermost layer 3 Record the hardware MAC address for example 0010835675AC The Error means the MAC address has not been assigned an IP address and the Tab File does not have this entry The MAC address is saved to the Tab File when an IP address is assigned 5 Close the log file before turning on another instrument 6 Uncheck the Do you want to log BootP requests box after configuring instruments to avoid having the logfile use up excessive disk space Agilent 1290 Infinity Binary Pump User Manual 279 13 LAN Configuration Adding each instrument to the network using BootP 1 Follow Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 Uncheck the Do you want to log BootP requests once all instruments have been added The Do you want to log Boot
172. unter limit exceeded Error ID 22056 Error Information The counter for the waste volume has exceeded the limit which has been set in the user interface e Parameter None Probable cause 1 The waste container is full 2 Inappropriate setting for waste counter Flow rate limit exceeded Error ID 22064 Suggested actions Empty waste container e Reset waste counter e Adjust waste counter limit The flow rate of the binary pump has exceeded the limit while the pump runs in pressure controlled mode e g during a pressure test Parameter None Probable cause 1 Leak 2 Bottle empty 3 Shutoff valve closed 4 Drift of pressure sensor unlikely for short tests taking some minutes Agilent 1290 Infinity Binary Pump User Manual Suggested actions Check for leaks in the pump and flow path Fill solvent bottle Open shutoff valve Replace pressure sensor 105 7 7 Error Information Binary pump shutdown during analysis Error ID 22065 The binary pump has been shut down by the control software or control module during an analysis e Parameter 0 for off 1 for standby Probable cause Suggested actions 1 Pump has been shut down Restart pump Reading the pump encoder tag failed Error ID 22402 Reading the pump encoder tag has failed e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent s
173. ure 20 on page 141 gives an overview of the torques that need to be set during maintenance of the pump heads IT Figure 20 Overview of torques for pump head procedures Agilent 1290 Infinity Binary Pump User Manual 141 9 Maintenance Replacing the Inlet valve Replacing the Inlet valve When If Inlet valve is defective Tools required p n Description Wrench 14 mm G4220 20012 Torque wrench 2 25 Nm Parts required p n Description G4220 60022 Inlet valve primary pump head NOTE For best performance and life time and for avoiding leaks use a torque wrench set to 10 Nm for fixing the inlet valve 1 Close the shut off valves to avoid solvent leaks 2 Unscrew the tubing at the inlet valve 142 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Inlet valve 3 With a 14 mm wrench unscrew the inlet valve and 4 Install inlet valve and tighten it at 10 Nm with a torque remove it wrench 14 mm 6 Open the shut off valves and purge the system to remove air Agilent 1290 Infinity Binary Pump User Manual 143 9 Maintenance Replacing the Outlet Valve Replacing the Outlet Valve When If Outlet valve is defective Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2603 G4220 20012 64220 20041 Parts required p n
174. ushing it to the rear then pull it out from the rear 6 Check the pump pistons for scratches grooves and dents Piston surface 164 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 7 Use the steel side of the insert tool for removing the pump seal from the pump chamber housing Insert tool Pump seal Do not use the pump piston for that purpose as this can break it Agilent 1290 Infinity Binary Pump User Manual 8 To flush out particles from the pump head use pressurized air 1 2 and 3 Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection 165 9 Maintenance 9 To remove wear use isopropanol 11 To dry the pump head and to remove residues use 10 To clean the piston and the pump head sufrace use the pressurized air 1 2 and 3 ultra fine abrasive mesh p n 8660 0852 166 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Disassembling the Secondary Pump Head VTE Ty Damage of pump piston The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 Remove the filter assembly at the top of the pump head 2 For disa
175. valve tag is accessed read write and used for correctly positioning the valve If the valve head is replaced while the pump is on invalid information may be written to the valve head making it unusable or positioning may be wrong resulting in wrong flow connections inside the valve potentially damaging parts gt Switch off the pump before working on the purge valve CAUTION Bias measurement results The valve drive contains sensitive optical parts Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Protect the optical parts from dust and other pollutions 188 Agilent 1290 Infinity Binary Pump User Manual Maintenance 9 Replacing the Purge Valve Head 1 Remove all capillary connections Then unscrew the 2 Put the new valve head onto the valve drive such that the black union nut and remove the head of the purge valve lobe fits to the groove Screw the valve head onto the by pulling it to the front valve drive using the union nut Agilent 1290 Infinity Binary Pump User Manual 189 9 Maintenance Replacing the Purge Valve Head 3 Install all flow connections Port 1 is connected to the outlet of the secondary pump head of channel B Port 2 is connected to the outlet of the pressure sensor Port 3 is connected to the inlet of the pressure sensor Port 4 is connected the outlet of the secondary pump head of channel A Ports 5 and
176. ve encoder has generated no signal Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Pump drive error Error ID 22438 22439 The pump drive failed during calibration Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive motor defect or mechanically Please contact your Agilent service blocked representative Agilent 1290 Infinity Binary Pump User Manual 115 7 Error Information Pump drive stroke blocked Error ID 22441 During initialization the pump defines the operation position of the pump drives and therefore the pistons First the pump drive moves backwards to find a mechanical stop within the ball screw Afterwards pistons move forwards for finding the maximum available stroke volume These values are expected within a pre defined range Maximum stroke too short means that the outer drive position is too close This can be caused by a drive initialization without pump head or if the pump head has not been installed properly screws are loose e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Wiper shifted Please contact your Agilent service representative 2 Pump head blocks piston movement Replace clean or repair pump head 3 Pump drive motor is mechanically blocked Please contact your Agilent service representative Pump dr
177. ved while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative Agilent 1290 Infinity Binary Pump User Manual 101 7 Error Information Pump Error Messages These errors are pump specific Pressure of binary pump above upper limit Error ID 22014 The pressure has exceeded the upper pressure limit e Parameter Measured pressure Probable cause Suggested actions 1 Blockage in flow path after the pressure Check for blockages in the LC system e g sensor purge valve Jet Weaver degraded column column frits needle needle seat capillaries etc Check for particles in the solvent 2 Inappropriate settings pressure limit flow Decrease flow rate rate e Increase pressure limit 102 Agilent 1290 Infinity Binary Pump User Manual Pressure below lower limit Error ID 22015 Error Information The pressure has dropped below the lower limit Parameter None Probable cause 1 Leak 2 Bottle empty 3 Wrong solvent viscosity 4 Inappropriate setting 5 Column degradation Suggested actions Check for leaks Check bottle filling Check solvent Check flow rate and lower pres
178. wer cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Agilent 1290 Infinity Binary Pump User Manual 21 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 23 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the modul
179. x current of primary pump drive too high 113 current of secondary high 114 D pump drive too defect connection between main board and pump drive encoder 110 defect on arrival 28 degasser s pressure limit violation 104 delay volume description 78 deliver underrun 11 delivery checklist 2 DHCP general informat setup 27 1 dimensions 23 eS ion 270 drive current too high drive current too low drive encoder error drive Encoder failed drive position limit drive timeout 109 E 108 108 112 108 T2 electrical connections descriptions of EMF 247 early maintenance feedback 260 error messages binary pump shutdown during analysis 106 compensation sensor open 98 299 Index compensation sensor short 99 cover violation 101 current of primary pump drive too high 113 current of secondary pump drive too high 114 defect connection between main board and pump drive encoder 110 degasser s pressure limit violation 104 deliver underrun 110 drive current too high 108 drive current too low 108 drive encoder error 112 drive Encoder failed 108 drive position limit 112 drive timeout 109 fan failed 99 flow rate limit exceeded 105 ignition without cover 100 100 insufficient power of drive encoder LED 112 eak sensor open 98 eak sensor short 97 eak 100 ost CAN partner 97 overcurrent of pump drive 109 overcurrent of solvent selection valve SSV 109 pressure below l
180. ysis by entering a time in the Time field and appropriate values in the following fields of the timetable Changes in flow rate occur linearly from either time zero or the time of the last defined change other parameters change instantaneously at the time defined in the timetable Show Advanced Timetable toggles the timetable display between standard mode and advanced mode The following parameters can be changed Change Contacts Change Flow Change Max Pressure Limit Change Solvent Composition You can only use solvents which have been enabled in the solvents section Function centric view This checkbox allows you displaying parameter changes instead of a time table Agilent 1290 Infinity Binary Pump User Manual 63 4 Using the Pump Priming the Pump When the solvents have been exchanged or the pumping system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel between the solvent reservoir vacuum degassing unit when available in the system and the pump Solvents containing volatile ingredients will slightly lose these Therefore priming of the pumping system is required before starting an application 1 Initiate a purge in the controlling software with a Purge flow set to 3 5 ml min per channel 2 Flush all tubes with at least 30 ml of solvent Table 7 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an

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