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Agilent 1290 Infinity Quaternary Pump

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1. 132 1290 Infinity Quaternary Pump User Manual Maintenance Replacing the Pressure Sensor 4 Connect the new pressure sensor to the pressure sensor connector 6 Connect the capillary from the pump head outlet to the pressure sensor inlet Two arrow signs on the pressure sensor indicate the flow direction 5 Fix the pressure sensor to the instrument chassis 7 f applicable connect the outlet of the pressure sensor to the central port of the Multi Purpose Valve 1290 Infinity Quaternary Pump User Manual 133 9 Maintenance Re he Inlet Weaver Replacing the Inlet Weaver Parts required p n Description G4204 81090 1290 Infinity Quaternary Pump Inlet Weaver Assembly Preparations Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages For easy access to the inlet weaver assembly remove tubing connections between MCGV and degasser 1 Open the screw at the bottom of the inlet valve 134 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Inlet Weaver 2 Open the fitting at the center of the multi channel 3 Pull the inlet weaver out of the inlet valve gradient valve MCGV Do not open the screw marked with the red cross Remove the inlet weaver from the MCGV
2. 178 1290 Infinity Quaternary Pump User Manual Maintenance 9 Assembling the Pump Head When Before installing the pump head Tools required p n G4220 20012 64220 20013 G4220 20041 G4220 20015 01018 23702 Parts required p n 0905 1719 Description Pump head alignment tool Torque wrench 2 25 Nm 4 mm hex bit Bit Torx 10x25 mm Adapter in square to hex Insert tool Description PE Seal See chapter Parts for details IETT Damage of the pump piston The pump piston is very sensitive to shearing forces from the side gt Use the alignment piston of the pump head alignment tool for the alignment CAUTION Wrong orientation of pins on support ring procedure described below Assembling the pump head without paying attention to the correct orientation of the pins on the support ring can lead to leaks or damage of the piston and pump head gt Observe pins on the support ring which help assembling the parts of the pump head in the correct orientation 1290 Infinity Quaternary Pump User Manual 179 9 Maintenance CAUTION Damage of the pump head assembly When installing the pump head assembly the pump drives need to be in maintenance position where they are retracted Using the pump drive in default position will damage the pump head assembly gt Bring the pump drive to the maintenance position CAUTION Damage to the pump head Using a wrong torque will damage the pump
3. APG Remote bxiernal CAN valves sinj Configuration switch amp c Ceme gr J o D Figure 32 Rear of quaternary pump 252 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table6 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes CAN DC OUT for CAN G4204A Quat Pump slaves G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B K1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B K1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes 1290 Infinity Quaternary Pump User Manual 253 12 Hardware Information Table6 Agilent 1200 Infinity S
4. 262 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicate
5. connection capillary Secondary pump head 2 Remove both pump heads from the link plate by pushing the clips at the rear of the plate Link plate Clips 164 1290 Infinity Quaternary Pump User Manual Maintenance 9 Disassembling the Primary Pump Head CAUTION Damage of pump piston The pump piston is made of Zr04 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 Remove the outlet valve at the top of the pump head and the inlet valve at the bottom of the primary pump head Outlet valve Inlet valve 1290 Infinity Quaternary Pump User Manual 165 9 Maintenance Disassembling the Primary Pump Head 2 For disassembling the pump head remove the 4 hex screws at the rear of the pump head 3 Remove the front part of the pump head including pump chamber housing with pump seal and support ring Seal holder including backup ring PE seal Pump chamber housing D gt 4 Remove the front part of the pump head including pump chamber housing with pump seal and support ring If the seal wash option is installed also remove the seal holder including backup ring and the gasket Support rin with wash geal Seal wash gasket Seal holder including ackup ring
6. 4 Insert the new inlet weaver to the inlet valve Fix the weaver with the plastic screw 6 Reconnect tubings between MCGV and degasser 1290 Infinity Quaternary Pump User Manual 135 9 Maintenance Replacing the Inlet Valve When If Inlet valve is defective Tools required p n Description Wrench 14mm G4220 20012 Torque wrench 2 25 Nm Parts required p n Description G4204 60022 Inlet Valve 1290 Infinity Quaternary Pump Preparations Switch off pump at the main power switch Remove the front cover e Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages Remove the inlet weaver see Replacing the Inlet Weaver on page 134 1 With a 14 mm wrench unscrew the inlet valve and remove it 0M a 136 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Inlet Valve 2 Install the new inlet valve and tighten it using a torque Next Steps wrench with a 14 mm bit set to 10 Nm 3 Insert the inlet weaver see Replacing the Inlet Weaver on page 134 4 Purge the system to remove air 1290 Infinity Quaternary Pump User Manual 137 9 Maintenance Replacing the Outlet Valve When Tools required Parts required Preparations 138 If Outlet valve is defective p n 8710 0510 8710 2603 G4220 20012 G4220 20041 p n G4220 60028 G422
7. Notices Agilent Technologies Inc 2012 2014 2015 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64204 90001 Edition 04 2015 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under
8. SO RRN Multi Purpose Valve 1290 Infinity Quaternary Pump User Manual 209 9 Maintenance Preparing the Pump Module for Transport 10 Carefully insert the protective foam to the front part of the instrument Do not damage any tubing or capillary connections 11 Close the front cover 12 For transport or shipment put the module and accessory kit to the original shipment box 210 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 10 Parts and Materials Overview of Main Assemblies 212 Flow Connections 214 Seal Wash Function 215 Pump Head Assembly Parts 216 Primary Pump Head Parts 218 Secondary Pump Head Parts 222 Multi Purpose Valve 225 Solvent Cabinet 226 Cover Parts 228 Leak Parts 229 Accessory Kit 230 Others 231 HPLC System Tool Kit 231 1290 Infinity Pump Service Kit 232 This chapter provides information on parts for maintenance ot Agilent Technologies 211 10 Parts and Materials Overview of Main Assemblies Overview of Main Assemblies Tt SS J WNS 7 e E Figure 18 Overview of maintenance parts 212 1290 Infinity Quaternary Pump User Manual Item p n 1 5067 4174 2 5067 5407 5023 0271 G4204 40000 5067 5416 3 G4204 60200 OR 64204 60400 OR 4 G4204 81090 OR 5067 5443 5 G1311 67701 5041 8365 6 G1311 60070 7 5065 4445 8 G4220 60001 9 G4204 68000 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Descr
9. Pump chamber housing 166 5 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 1290 Infinity Quaternary Pump User Manual Maintenance 9 Disassembling the Primary Pump Head 6 Check the pump pistons for scratches grooves and 7 Use the steel side of the insert tool for removing the dents when changing the piston seals pump seal from the pump chamber housing NOTE Damaged pistons cause micro leaks and will decrease the lifetime of the seals Piston surface Pump seal NOTE Do not use the pump piston for that purpose as this can break it 1290 Infinity Quaternary Pump User Manual 167 9 Maintenance 8 To flush out particles from the pump head use 9 To remove wear use isopropanol pressurized ait 1 2 and 3 10 To clean the piston and the pump head surface use the ultra fine abrasive mesh p n 8660 0852 Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection 168 1290 Infinity Quaternary Pump User Manual Maintenance 9 11 To dry the pump head and to remove residues use pressurized air 1 2 and 3 1290 Infinity Quaternary Pump User Manual 12 If the wash seal shall be replaced by a new one use the steel side of the insert tool for removing it Wash seal 169 9 Maintenanc
10. 1 Defect cable connection to seal wash pump Check cable connection 2 Defect seal wash pump motor Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Valve hardware overcurrent MCGV Error ID 29227 Power consumption too high for one of the MCGV valves Probable cause Suggested actions 1 Cable defect Replace MCGV 2 Valve defect Replace MCGV 3 Defective main board Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual 119 7 Error Information Pump Error Messages 120 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual This chapter will describe the tests for the module Currently no diagnostic procedures are available ott Agilent Technologies 121 8 Test Functions and Calibrations Pump Error Messages 122 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 9 Maintenance Introduction to Maintenance 125 Warnings and Cautions 126 Overview of Maintenance 128 Cleaning the Module 129 Installing Fittings and Capillaries 130 Replacing the Pressure Sensor 131 Replacing the Inlet Weaver 134 Replacing the Inlet Valve 136 Replacing the Outlet Valve 138 Removing the Jet Weaver 141 Installing the Jet Weaver 144 Replacing the Seal Wash Pump 146 Replacing the Multi Channel Gradient Valve MCGV 148 Releasing a
11. 125 9 Maintenance Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 126 1290 Infinity Quaternary Pump User Manual Maintenance 9 CAUTION Safety stand
12. P lyf es external CAN valves E Configuration switch E YE naga O CEme CAN Bus E im E RS232 E roma fees TAREE USB port LAN E 5 z gt Power plug a DOD sgg 5 ge O a Figure 14 Rear view of the quaternary pump In an Agilent 1290 Infinity System the individual modules are connected by CAN cables An Agilent 1200 Series Instant Pilot can be connected to the CAN bus of any module Connection to an Agilent data system is established through the built in LAN port of the detector The LAN port of the detector must be used as the detector generates the highest data rate of all modules For more information about connecting the Instant Pilot or Agilent Data System please refer to the respective user manual For setting up the LAN access see LAN Configuration on page 265 5 Turn on the power by pushing the button at the lower left hand side of the module The power button stays pressed in and the status LED should be green NOTE When the line power button stands out and the green light is off the module is turned off NOTE The module was shipped with default configuration settings For changing these settings refer to section Setting the 8 bit configuration switch 1290 Infinity Quaternary Pump User Manual 41 3 Installing the Module Flow Connections to the Pump Flow Connections to the Pump Multi purpose valve Pressure sensor Seal wash pump Degasser Inlet weaver Pump heads T
13. Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation 1290 Infinity Quaternary Pump User Manual 297 14 Appendix 298 Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable
14. System Info Controller DE12345678 G4208A Main Revision B 02 12 0001 DAD DE64260019 G1315D Main Revision B 06 41 0002 a Resident Revison _B 06 40 0007 A print On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 LAN MAC Address _0030D314F89E Board ID TYPE G1315 66565 SER MAC REV AC MFG Lamp 2140 0820 848728 Cell no info Exit v information on each module a 10 08 Figure 43 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules Table 15 G1369C LAN Interface Card configuration switch on the card SW 4 SW 5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP 274 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Table 16 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation Lab Advisor Firmware Update Tool and use MAC address as host name e g 003043177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 273 1290 Infinity Quaternary Pump User Manual 275 13 LAN Configuration Link Configuration Selection The L
15. 9 Maintenance Assembling the Pump Head 4 Loosely close the 4 screws at the rear of the pump head 5 Insert the alignment piston of the pump head alignment The screws will be fixed tightly later tool Lubricate the alignment piston with isopropanol or another suitable solvent before insertion CID Alignment piston 182 1290 Infinity Quaternary Pump User Manual Maintenance 9 CAUTION Damage to the pump head gt Using the alignment tool is mandatory gt Not using will break the pump head 6 Insert the pump head to the pump head alignment tool which is included to the 1290 Infinity Service Kit p n 5067 4699 There are openings for the seal wash support ring and heat exchanger of the secondary pump head Observe the correct orientation of all parts Pump head alignment tool rear AQ Pump head alignment tool front 7 Close the tool by closing the three screws at the connection ring Heat exchanger capillary 1290 Infinity Quaternary Pump User Manual 183 9 Maintenance CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 8 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the central alignment screw CAUTION Damage to the pump head Using a wr
16. Based on retention time at constant room temperature lt 0 15 RSD or 0 02 min SD whatever is greater 1 mL min 0 40 absolute 1 99 B 0 5 2 0 mL min with water caffeine tracer 400 bar Number of channels 4 Internal volume per channel 1 5 mL Agilent ChemStation for LC C 01 04 or above OpenLAB A 04 04 Masshunter B 05 01 or above Agilent Instant Pilot G4208A B 02 08 or above Controller area network CAN RS232C APG remote ready start stop and shutdown signals LAN Extensive diagnostics error detection and display through Agilent LabAdvisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable 1290 Infinity Quaternary Pump User Manual 27 2 Site Requirements and Specifications Performance Specifications 28 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 3 Installing the Module Unpacking the Module 30 Optimizing the Stack Configuration 31 One Stack Configuration 31 Two Stack Configuration 34 Installation Information on Leak and Waste Handling 36 Removing the Transport Foam 39 I
17. From pump To waste Inline filter Figure 5 Valve position in filter flush mode CAUTION Damage to the valve gt Use the filter flush mode only if the optional inline filter is installed 1290 Infinity Quaternary Pump User Manual 17 1 Introduction System Overview System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed Figure 6 Leak and waste handling concept overview typical stack configuration as an example 18 1290 Infinity Quaternary Pump User Manual Introduction 1 The solvent cabinet 1 is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet The leak pan 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 8 A guides excessive overfill from one module to th
18. One pump head assembly consists of two pump heads including valves and the outlet filter which are both removed at the same time 156 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Pump Head 1 In Lab Advisor go to Tools gt Remove Install Pump Head 3 DO NOT REMOVE the capillary connection between the and follow instructions given on the screen pump heads marked by the red X 2 If applicable remove flow connections of the seal wash option 1290 Infinity Quaternary Pump User Manual 157 9 Maintenance Replacing the Pump Head 4 Remove the capillary connection from the outlet filter on 5 Open the black plastic screw of the inlet valve at the the secondary pump head to the pressure sensor bottom of the left primary pump head 1 and remove the inlet weaver by first pushing it downwards 2 and then pulling it out to the left 3 Connection to inlet weaver 158 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Pump Head 6 Open the four screws holding the pump heads NOTE Open all screws step by step not screw by screw 1290 Infinity Quaternary Pump User Manual 7 Remove the complete pump head assembly by holding both heads and pulling it to the front Do not further disassemble the pump head 159 9 Maintenance Replacing the Pump Head 8 Use a new pre tested pump head assembly including C
19. The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address Determining the MAC address of the instrument using BootP Service 1 Power cycle the Instrument 2 After the instrument completes self test open the log file of the BootP Service using Notepad The default location for the logfile is C Documents and Settings All Users Application Data Agilent BootP LogFile The logfile will not be updated if it is open The contents will be similar to the following 02 25 10 15 30 49 PM Status BootP Request received at outermost layer Status BootP Request received from hardware address 0010835675AC Error Hardware address not found in BootPTAB 0010835675AC Status BootP Request finished processing at outermost layer 3 Record the hardware MAC address for example 0010835675AC The Error means the MAC address has not been assigned an IP address and the Tab File does not have this entry The MAC address is saved to the Tab File when an IP address is assigned 5 Close the log file before turning on another instrument 6 Uncheck the Do you want to log BootP requests box after configuring instruments to avoid having the logfile use up excessive disk space 282 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Adding each instrument to the network using BootP 1 Follow Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings
20. contains p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20014 2 5 mm Hex Bit G4220 20015 Adapter in square to hex G4204 44000 Transport protection foam 5023 0285 Replacement kit for 1290 Infinity pump head alignment tool piston handle 232 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 11 Identifying Cables Cable Overview 234 Analog cables 236 Remote Cables 238 BCD Cables 241 CAN LAN Cable 243 RS 232 Cable Kit 244 Agilent 1200 Module to Printer 245 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ott Agilent Technologies 233 11 Identifying Cables Cable Overview 234 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 238 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpos
21. www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity Quaternary Pump G4204A The manual describes the following Introduction e Site requirements and specifications e installation e configuration e using and optimizing e troubleshooting and diagnostic e error information e test functions e maintenance e parts identification e hardware information e safety and related information Agilent Technologies 2012 2014 2015 Printed in Germany 04 2015 64204 90001 a Agilent Technologies
22. Default Gateway not specified 1290 Infinity Quaternary Pump User Manual 271 13 LAN Configuration 272 Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user they are still available when switching back to the mode Using Stored 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Dynamic Host Configuration Protocol DHCP General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to
23. Insert a new filter frit p n 5067 5716 in the correct orientation observe different thickness of holder on either side Inner side Outer side Technical drawing Actual color Filter assembly Filter frit CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 13 For the secondary pump head install the outlet filter using the torque wrench 14 mm hex bit which is included to the 1290 Infinity Service Kit p n 5067 4699 set to 16 Nm 186 1290 Infinity Quaternary Pump User Manual Maintenance 9 Assembling the Pump Head 14 Remove the alignment piston 15 Lubricate the pump piston with isopropanol or another suitable solvent and insert it Piston 1290 Infinity Quaternary Pump User Manual 187 9 Maintenance Assembling the Pump Head 16 Insert both pump heads to the link plate and make sure that the clips snap in that fix the pump heads Link plate NOTE Observe the correct orientation of the primary and the secondary pump head This is important for correct fixation of the heat exchanger and the capillaries as described in the following steps NOTE Mind the orientation of the ID tag 17 Bring the pump drive to the maintenance position using the Lab Advisor user interface see Replacing the Pump Hea
24. Probable cause Suggested actions 1 Defect encoder Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual 109 7 Error Information Multi Purpose Valve failed Error ID 29231 Lost steps of the purge valve encoder Parameter None Probable cause Suggested actions 1 Multi purpose valve drive mechanically e Check installation of multi purpose valve blocked or defect head e Replace multi purpose valve head Reading of multi purpose valve tag failed Error ID 29240 Reading the multi purpose valve tag failed e Parameter None Probable cause Suggested actions 1 Reading of multi purpose valve tag failed Check cable connection 2 Multi purpose valve head tag defect or Replace multi purpose valve head empty 3 Multi purpose valve tag reader is defect Please contact your Agilent service representative 110 1290 Infinity Quaternary Pump User Manual Error Information 7 Pump drive encoder rollover Error ID 29232 Invalid pump drive encoder signals have been detected Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Drive position limit Error ID 29234 Internal error Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Internal error Please contact your Agilent service representative Insufficient power of drive encoder LED
25. download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does not offer any services for hostname resolution e g NetBIOS DHCP Active Server Parameter Figure 42 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions 1290 Infinity Quaternary Pump User Manual 273 13 LAN Configuration Setup DHCP Software required The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section
26. percentage at start conditions before a gradient Click the down arrow and select the mixer to use from the list Use any mixer The currently installed mixer is used irrespective of its type Do not use mixer The valve is set to bypass the mixer so that it is not in the flow path lt Mixer Name gt Only the specified mixer may be used it the mixer is not found the pump goes into a Not Ready state 64 Timetable Settings Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable Changes in flow rate occur linearly from either time zero or the time of the last defined change other parameters change instantaneously at the time defined in the timetable Show Advanced Timetable toggles the timetable display between standard mode and advanced mode The following parameters can be changed Change Contacts Change Flow Change Max Pressure Limit Change Solvent Composition You can only use solvents which have been enabled in the solvents section Function centric view This checkbox allows you displaying parameter changes instead of a time table 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Blend Assist The Blend Assist table allows you to blend two or more solvents or solvent mixtures from stock solutions The blends must be of pure solvents or pure solvents wi
27. 1290 Infinity Quaternary Pump Head Assembly without Seal Wash Switch off pump at the main power switch e Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages Limitation of life time The pump head assembly is an exchange part which cannot be reassembled with standard tools Disassembling the pump head will strongly limit its life time gt Do not disassemble the pump head assembly CAUTION Damage of connections Disassembling the flow connection between the two pump heads of the pump head assembly solvent channel can damage the connection and cause leaks gt Do not disconnect the flow connection between the pump heads CAUTION Damage of internal parts gt Do not apply a strong force to the screws of the pump head gt Use a torque hex key for that purpose 1290 Infinity Quaternary Pump User Manual 155 9 Maintenance Verh Damage of the pump piston Removing pump heads in a position other than the maintenance position can damage the pump piston gt Before switching off the pump bring it to the maintenance position CAUTION Damage of pump drives The pump drive can be damaged if the pump initializes after switching it on without having the pump head installed properly gt Use the Lab Advisor maintenance procedure for replacing pump heads gt Install the pump head correctly before switching on the pump
28. 130 The lifetime of a fitting depends on how firmly it has been tightened firm tightening reduces the lifetime If fitting has been overtightened replace it 1 Install fittings and capillaries 2 Tighten fittings and capillaries 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Pressure Sensor When Tools required Parts required Preparations No or invalid pressure signal p n Description 8710 2412 Hex key 2 5 mm 15 cm long straight handle 8710 0510 Wrench open 1 4 5 16 inch Screwdriver p n Description 1 G4220 60001 Pressure sensor 1200 bar Turn off pump flow switch off pump This procedure describes how to replace the pressure sensor In case the cable to the sensor shall be replaced as well please contact your Agilent service representative Working on connections to the pressure sensor may slightly change the displayed pressure In case of a pressure offset at ambient pressure a pressure offset calibration may be run 1290 Infinity Quaternary Pump User Manual 131 9 Maintenance Replacing the Pressure Sensor 1 Remove capillary connections between the pressure sensor and the Multi Purpose Valve and between the pressure sensor and the outlet filter of the secondary pump head respectively 2 Remove the screws that fix the pressure sensor to the 3 Carefully pull out the pressure sensor for about 2 cm chassis Then unscrew the cable from the pressure sensor
29. Displays the pump s Control dialog box ee Method Displays the pump s Method Setup dialog box Set Error Method Sets the method that is loaded if an error occurs to the method that is currently available in the hardware Identify Device Causes the LED on the front of the module to blink for a few seconds z Switch Pump On Off Toggles the status of the pump on or off I Bottle Fillings Displays the Bottle Fillings dialog box Purge On Off Allows you to control the purging of the system Prime On Off Allows you to prime the pump heads for initially drawing solvent Conditioning On Off Allows you to switch pump conditioning on and off The conditioning function is useful for removing small air bubbles inside the pump flow path Flush Filter On Off Allows you to flush a clogged inline filter which is connected to the Multi Purpose Valve see Filter Flush Mode on page 17 Use the pump self test for checking the filter back pressure Do not use this option if no filter is installed 1290 Infinity Quaternary Pump User Manual 57 4 Using the Pump Control Settings The Quaternary Pump control parameters are in six sections Pump Seal Wash Automatic Turn On e Purge e Prime e Conditioning Table 3 Pump control parameters Parameter Limits Description Pump Enables you to switch the pump On Off or to a Standby condition In the Standby condition the pump motor is still active and when the pump is swit
30. SSV to shutoff valve or degassing unit to MCGV G4220 60017 Bottle Head Assembly Ultra Clean Tubing bottle heads and tubing to shutoff panel degasser 214 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Seal Wash Function Figure 20 Seal Wash Pump p n Description 5065 4445 5065 9978 Peristaltic pump with Pharmed tubing Tubing 1 mm i d 3 mm o d silicone 5 m 1290 Infinity Quaternary Pump User Manual 215 10 Parts and Materials Pump Head Assembly Parts Pump Head Assembly Parts Figure 21 Pump head assembly parts 216 1290 Infinity Quaternary Pump User Manual Item p n 1 G4204 60200 1 64204 60400 1 1 G4220 81013 2 1 G4220 40001 3 1 64204 60022 4 1 G4220 60028 5 1 G4220 20020 6 1 64204 60004 7 4 G4220 23704 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Description 1290 Infinity Quaternary Pump Head Assembly with Seal Wash 1290 Infinity Quaternary Pump Head Assembly without Seal Wash Heat Exchanger secondary pump head only Link Plate Inlet Valve 1290 Infinity Quaternary Pump Outlet valve primary pump head Internal gold seal for Outlet Valve Outlet filter 1290 Infinity Quaternary Pump Stay bolt 217 10 Parts and Materials Primary Pump Head Parts Primary Pump Head with Seal Wash Quaternary Pump Figure 22 Primary pump head Quaternary Pump with seal wash 218 1290 Infinity Quaternary Pump User Manual Item 10 12 1
31. Stuck Inlet Valve 152 Replacing the Pump Head 155 Disassembling the Pump Head 163 Disassembling the Primary Pump Head 165 Disassembling the Secondary Pump Head 170 Replacing the Heat Exchanger 174 Replacing Wash Seal and Gasket 177 Assembling the Pump Head 179 Replacing the Multi Purpose Valve 191 Replacing Parts of the Multi Purpose Valve 194 Replacing the Outlet Filter 196 Installing the Inline Filter 198 ote Agilent Technologies 123 9 Maintenance Removing the Inline Filter 200 Replacing Parts of the Inline Filter 202 Installing the Valve Rail Kit 205 Replacing Module Firmware 206 Preparing the Pump Module for Transport 207 This chapter describes the maintenance of the Agilent 1290 Infinity Quaternary Pump 124 1290 Infinity Quaternary Pump User Manual Maintenance Introduction to Maintenance Introduction to Maintenance Figure 17 on page 125 shows the main user accessible assemblies of the Agilent 1290 Infinity Quaternary Pump These parts can be accessed from the front simple repairs and don t require to remove the pump from the system stack Multi Purpose Valve aes tam ataty DEA Po Jet Weaver optional Pressure sensor Seal wash pump Degasser Multi channel gradient valve MCGV Inlet weaver Pump head assembly Inline filter optional Figure 17 Overview of Maintenance Parts 1290 Infinity Quaternary Pump User Manual
32. When Tools required Preparations CAUTION If parts inside the pump head need to be replaced p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20014 2 5 mm Hex Bit G4220 20015 Adapter in square to hex 8710 0510 Wrench open 1 4 5 16 inch Remove the pump head assembly as described in Replacing the Pump Head on page 155 Damaged pump head Disassembling or reassembling the pump head with tools other than the ones recommended can damage pump heads and significantly reduce their life time gt Follow all instructions step by step gt Use recommended tools like the pump head alignment tool and a torque wrench This section describes the maintenance of the 1290 Infinity Quaternary Pump head Agilent recommends using refurbished pump heads for maintenance and repair instead of disassembling pump heads Such pump heads are available through Agilent service The 1290 Infinity pump service kit 5067 4699 includes all tools required for the procedures described in subsequent sections The primary pump head does not have a heat exchanger Seal wash parts are optional for both pump heads 1290 Infinity Quaternary Pump User Manual 163 9 Maintenance Disassembling the Pump Head 1 Open the 2 5 mm hex or Tx 10 screw at the top of the primary pump head which fixes the connection capillary of the heat exchanger Then lift up the capillary and remove it from the primary pump head
33. Wrong solvent viscosity Check solvent 4 Inappropriate setting Check flow rate and lower pressure limit 5 Column degradation Replace column 102 1290 Infinity Quaternary Pump User Manual Error Information 7 Target pressure not reached for quaternary pump degasser Error ID 29221 The target pressure of the quaternary pump degasser has not been reached within the expected time Parameter Pressure in mbar Probable cause Suggested actions 1 Condensation in degasser chamber due to Equilibrate and restart module temperature fluctuation 2 Degasser is defect Please contact your Agilent service representative Solvent counter exceeded limit Error ID 29146 The counter for the solvent volume has exceeded the limit which has been set in the user interface Probable cause Suggested actions 1 No solvent present Refill solvent bottle 2 Inappropriate setting Check solvent counter setting in user interface 1290 Infinity Quaternary Pump User Manual 103 7 Error Information Waste counter limit exceeded Error ID 29147 The counter for the waste volume has exceeded the limit which has been set in the user interface e Parameter None Probable cause Suggested actions 1 The waste container is full Empty waste container 2 Inappropriate setting for waste counter e Reset waste counter e Adjust waste counter limit Flow rate limit exceeded Error ID 29164 The flow rate of the quaternary pump has exceeded the
34. be set to store the IP address and will not lose the IP address if power cycled Situation Cannot Establish LAN Communication If a LAN communication with BootP service cannot be established check the following on the PC e Is the BootP service started During installation of BootP the service is not started automatically Does the Firewall block the BootP service Add the BootP service as an exception e Is the LAN Interface using the BootP mode instead of Using Stored or Using Default modes 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Installation of BootP Service Before installing and configuring the Agilent BootP Service be sure to have the IP addresses of the computer and instruments on hand 1 2 3 Log on as Administrator or other user with Administrator privileges Close all Windows programs Insert the Agilent ChemStation software DVD into the drive If the setup program starts automatically click Cancel to stop it 4 Open Windows Explorer 5 Go to the BootP directory on the Agilent ChemStation DVD and double click BootPPackage msi If necessary click the Agilent BootP Service icon in the task bar The Welcome screen of the Agilent BootP Service Setup Wizard appears Click Next The End User License Agreement screen appears Read the terms indicate acceptance then click Next The Destination Folder selection screen appears Install BootP to the default folder or click
35. been set up in the Blend Assist section of the method setup Solvent The solvent or blend of solvents as set up in the Blend Assist section Used Mark this check box if you want to use this solvent or blend in the method Enter the percentage of the solvent or blend in this field Name Type a name for the solvent or blend in this field The stoptime sets a time limit for your analysis After the stoptime all gradients are stopped and the pump parameters return to their initial values The pump can be used as a stoptime master for the complete analytical system The pump also stops the detectors if they have a No Limit stoptime setting If no limit is given a method will have to be stopped manually Your instrument remains in a not ready state during the posttime to delay the start of the next analysis You can use the Posttime to allow your column to equilibrate after changes in solvent composition for example after gradient elution 1290 Infinity Quaternary Pump User Manual 61 4 Using the Pump Table4 Method parameters Parameter Limits Description Pressure Limits Max 1200 bar Sets the maximum and minimum pressure limits for the pump 17400 psi for flow Max is the maximum pressure limit at which the pump will switch rates up to 2 mL min itself off protecting the analytical system against over pressure For flow rates Minis the minimum limit at which the pump will switch itself off for between 2
36. constant to ensure precise retention Recommended flow time and peak measurements Variations in flow rate can occur as a result range 0 05 of the partial failure of the pumping system air in the pumping system a 5 00 mL min change in the mobile phase viscosity or a temperature change Enable Blend Assist Mark this check box to switch on Blend Assist which allows you to set up solvent mixtures from stock solutions When this check box is marked the Blend Assist section of the method setup is available 60 1290 Infinity Quaternary Pump User Manual Table 4 Method parameters Using the Pump 4 Parameter Limits Description Solvents Stoptime Posttime 0 01 99999 min or As Injector No Limit an infinite run time 0 01 99999 min or Off 0 0 min Blend Assist Disabled When Blend Assist is disabled you can set the percentages of solvents B C and D to any value from 0 through 100 Solvent A always delivers the remaining volume 100 B C D The check boxes allows you to turn the solvent channels on checked or off cleared Click the solvent name down arrow and select the solvent from the list of calibrated solvents and solvent mixtures For solvent mixtures specify the percentage of additive You can enter your own name for the solvent or solvent mixture in the adjacent field Blend Assist Enabled When Blend Assist is enabled the table shows the solvent blends that have
37. depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1290 Infinity Quaternary Pump User Manual 241 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 242 1290 Infinity Quaternary Pump User Manual CAN LAN Cable Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 1290 Infinity Quater
38. example The Tab File is located at C Documents and Settings All Users Application Data Agilent BootP TabFile 284 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Changing the IP Address of an Instrument Using the Agilent BootP Service Agilent BootP Service starts automatically when your PC reboots To change Agilent BootP Service settings you must stop the service make the changes and then restart the service Stopping the Agilent BootP Service 1 From the Windows control panel select Administrative Tools gt Services The Services screen appears RE Be aton yew teb s B iDRBI r gt r gt s n Select an tem to view ts description Figure 47 Windows Services screen 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools screen 1290 Infinity Quaternary Pump User Manual 285 13 LAN Configuration Editing the IP address and other parameters in EditBootPSettings 1 Select Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 When the BootP Settings screen is first opened it shows the default settings from installation 3 Press Edit BootP Addresses to edit the Tab File x Host Name IP Address Comment Gateway 006000111599 Agier LC1 1011 10 Agiert LET ngtt 285 255 2550 0000 005000222883 Ager C2 101 1 1 102 Agiert LC2 left 252552550 0000 Figure 48 Edit BootP Adresses scr
39. goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F 48 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For details on correct installation see section Installation Information on Leak and Waste Handling in the service manual 1290 Infinity Quaternary Pump Us
40. interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector s for signal output 1290 Infinity Quaternary Pump User Manual 255 12 Hardware Information 256 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the confi
41. is purged Purge Flow Time and Composition during purge have to be defined As soon as the duration time of the purge ends the module automatically switches to analytical conditions again Select On to start priming Off to turn priming off The Prime function is helpful for filling empty solvent lines or if air has entered the pump heads The module draws solvent at high speed with both pump drives simultaneously and dispenses it against the waste position of the Multi Purpose Valve This is done 20 times before the process comes to an end Use this function if you see excessive pressure or composition ripple and you are sure that the solvent type aqueous organic or specific solvent solvent mix is correctly set and there is no evidence of leakage in the pump Conditioning may be necessary if the pump may contain air for example after running out of solvent after a long period of standby or after service or repair 1290 Infinity Quaternary Pump User Manual 59 4 Using the Pump Method Parameter Settings The Quaternary Pump method setup parameters are in nine sections gt Flow Solvents A to D Stoptime e Posttime e Pressure Limits e Timetable e Advanced e Blend Assist e External Contacts Table4 Method parameters Parameter Limits Description Flow 0 00 5 00 mL min The flow is the rate of movement of eluent along the column It is in steps of 0 001 important that the flow rate is kept
42. limit while the pump runs in pressure controlled mode e g during a pressure test e Parameter None Probable cause Suggested actions 1 Leak Check for leaks in the pump and flow path 2 Bottle empty Fill solvent bottle 3 Shutoff valve closed if applicable Open shutoff valve 4 Drift of pressure sensor unlikely for short Replace pressure sensor tests taking some minutes 104 1290 Infinity Quaternary Pump User Manual Error Information 7 Quaternary pump shutdown during analysis Error ID 29199 The quaternary pump has been shut down by the control software or control module during an analysis e Parameter 0 for off 1 for standby Probable cause Suggested actions 1 Pump has been shut down Restart pump Reading the pump encoder tag failed Error ID 29201 Reading the pump encoder tag has failed e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent service main board representative 2 Missing or defect tag Defect connection Please contact your Agilent service between tag and encoder representative 1290 Infinity Quaternary Pump User Manual 105 7 Error Information Writing the pump encoder tag failed Error ID 29200 Writing the pump encoder tag has failed e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent ser
43. mL min example if any solvent reservoir is empty this prevents system and 5 mL min the damage by pumping air maximum pressure ramps down to 800 bar 11600 psi Min any value between 0 and the upper pressure limit setting Timetable See Timetable Settings on page 64 Advanced See Advanced Settings on page 63 External Contacts The External Contacts section enables you to set up the switching of the external contacts The External Contacts section is present only when a BCD external contacts board is installed 62 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Advanced Settings The Quaternary Pump advanced method setup parameters are in five sections Minimum Stroke e Compressibility Maximum Flow Gradient Primary Channel e Mixer Selection Table5 Advanced method parameters Parameter Limits Description Minimum Stroke 20 100 pL The Stroke Volume is used for optimizing between performance of the module and seal life time For performance a low stroke volume is beneficial as it divides disturbances into smaller packages but a larger volume is extending the life time of the pump seals If Automatic is activated the pump tries to achieve an optimized stroke volume for the Jet Weaver geometry Compressibility The compressibility of the mobile phase has an effect on the performance of the pump For best flow accuracy and mixing performance you can set the parameter according
44. on page 26 1290 Infinity Quaternary Pump User Manual Introduction 1 Overview of the Quaternary Pump The Agilent 1290 Infinity Quaternary Pump has a built in 4 channel vacuum degasser for best flow stability especially at low flow rates and maximum detector sensitivity It uses a multi channel gradient valve MCGV for formation of quaternary gradients at low pressure The low pressure Inlet Weaver based on patented Agilent microfluidic technology ensures highest mixing performance and lowest mixing noise The pump head offers a high power range with a maximum pressure of 1200 bar and a maximum flow rate of 5 mL min The Multi Purpose Valve can be used for automatic purging using an optional Jet Weaver high performance mixer automatic back flushing of the optional inline filter or for diagnostic The Agilent 1290 Infinity Quaternary Pump is suitable for a wide range of columns and HPLC und UHPLC applications starting from typical 250 x 4 6 mm HPLC columns going down to high resolution 50 x 2 1 mm UHPLC columns and can be used in a flow range between 0 05 5 mL min The active seal wash function can be used with concentrated buffer solutions 1290 Infinity Quaternary Pump User Manual 11 1 Introduction Operating Principle 12 The pump head comprises two pump chambers in series with independent high resolution motion control A pressure sensor in the flow path monitors the pressure The pump control uses this signal for
45. page 144 82 1290 Infinity Quaternary Pump User Manual How to Optimize the Performance of Your Module 5 How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section explains how resolution can be increased by examining the following points e Optimize selectivity e Smaller particle size packing Longer Columns e Shallower gradients faster flow Resolution between two peaks is described by the resolution equation 1 a 1 k Rs VN 2 i 4 a k where R resolution N plate count measure of column efficiency a selectivity between two peaks e ko retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selectivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an au
46. performance flow retention time stability a blockage or internal leak of the pump Avoid the use of such solvent combinations as they can cause irreproducible chromatographic results 1290 Infinity Quaternary Pump User Manual 79 5 How to Optimize the Performance of Your Module Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even though this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection The del
47. pressure limit violation 118 delay volume description 80 deliver underrun 109 delivery checklist 30 DHCP general information 273 setup 2 4 dimensions 25 disassembling primary pump head 165 pump head 163 secondary pump head 170 drive current too high 107 drive current too low 107 drive encoder error 112 drive encoder failed 117 drive phases differ too much in electric esistance 118 drive position limit 111 drive timeout 108 E electrical connections descriptions of 251 EMF early maintenance feedback 263 error messages compensation sensor open 99 303 Index compensation sensor short 100 defect connection between main board and pump drive encoder 109 degasser s pressure limit violation 118 deliver underrun 109 drive current too high 107 drive current too low 107 drive encoder error 112 drive encoder failed 117 drive phases differ too much in electric esistance 118 drive position limit 111 drive timeout 108 fan failed 101 flow rate limit exceeded 104 insufficient power of drive encoder LED 111 leak sensor open 99 leak sensor short 98 leak 101 lost CAN partner 98 maximum stroke too short 114 multi purpose valve failed 110 overcurrent of pump drive 108 pressure below lower limit 102 pressure exceeded upper pressure limit 102 pump drive blocked or encoder failed 106 pump drive encoder defect 109 ump drive encoder error 113 ump drive encoder rollover 111 ump drive error 1
48. rear of the module underneath the configuration switch see Figure 36 on page 266 G4204 65800 ae Part number of the pump main board AA ZZ 210 MSSA wis Se Revision code vendor year and week of assembly MAC 003xD31X6EXx byt MAC address Made in Germany Country of origin Figure 35 MAC label 2 Connect the instrument s LAN interface see Figure 36 on page 266 to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable C hyi ectemapet en LELLU ititi oponent Ta i Figure 36 Location of LAN interfaces and MAC label 266 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 TCP IP Parameter Configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 274 e by manually setting the parameters using Telnet e by manually setting the parameters using the I
49. screen appears 2 Uncheck the Do you want to log BootP requests once all instruments have been added The Do you want to log BootP requests box must be unchecked when you have finished configuring instruments otherwise the log file will quickly fill up disk space 3 Click Edit BootP Addresses The Edit BootP Addresses screen appears 4 Click Add The Add BootP Entry screen appears x Mac Address Host Name Figure 46 Enable BootP logging 1290 Infinity Quaternary Pump User Manual 283 13 LAN Configuration 5 Make these entries for the instrument e MAC address Host name Enter a Hostname of your choice The Host Name must begin with alpha characters i e LC1260 e IP address Comment optional e Subnet mask e Gateway address optional The configuration information entered is saved in the Tab File Click OK Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK After each modification of the BootP settings G e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 285 or Restarting the Agilent BootP Service on page 286 oo N O 10 Power cycle the Instrument OR If you changed the IP address power cycle the instrument for the changes to take effect 11 Use the PING utility to verify connectivity by opening a command window and typing Ping 192 168 254 11 for
50. security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets 36 1290 Infinity Quaternary Pump User Manual Installing the Module 3 Installation Information on Leak and Waste Handling Figure 11 Leak and waste handling overview typical stack configuration as an example S
51. to log BootP requests check box 1 Open BootP Settings from Start gt All Programs gt Agilent BootP Service gt EditBootPSettings 2 In BootP Settings check Do you want to log BootP requests to enable logging Logging MV Do you want to log bootP requests BootP Log File Sa TT EP Figure 45 Enable BootP logging The log file is located in C Documents and Settings All Users Application Data Agilent BootP LogFile It contains a MAC address entry for each device that requests configuration information from BootP 3 Click OK to save the values or Cancel to discard them The editing ends 4 After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 285 or Restarting the Agilent BootP Service on page 286 5 Uncheck the Do you want to log BootP requests box after configuring instruments otherwise the log file will quickly fill up disk space Determining the MAC address directly from the LAN Interface card label 1 Turn off the instrument 2 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module See Figure 35 on page 266 and Figure 36 on page 266 3 Turn on the instrument 1290 Infinity Quaternary Pump User Manual 281 13 LAN Configuration Assigning IP Addresses Using the Agilent BootP Service
52. to the mobile phase being used Use solvent types Select this check box recommended for using the enhanced and automatic compressibility calibration Then select the calibrated solvent from the drop down lists using the combo boxes in the Solvents section Using this checkbox hides compressibility fields for manual settings e Clear this check box to display the compressibility fields which allow you to enter manual compressibility values which are constant over pressure This setting is available for method backward compatibility e g from 1260 Infinity pumps For best performance use solvent types Maximum Flow 1 000 You can set a limit on the rate of change of the solvent flow to protect your Gradient 1000 000 mL min mi analytical column You can set individual values for Flow ramp up and n in steps of Flow ramp down 0 001 mL min min Default value 100 000 mL min min 1290 Infinity Quaternary Pump User Manual 63 4 Using the Pump Table5 Advanced method parameters Parameter Limits Description Primary Channel Mixer Selection Using Automatic is recommended The primary channel can be specified as A to D for optimizing highly specific methods It is split up to deliver the first and last solvent package created by the MCGV in order to optimize composition precision The primary channel does not change during a gradient as long as the channel is used Using Automatic chooses the channel with the highest
53. violation 118 Seal wash pump was missing when tried to turnon 119 Valve hardware overcurrent MCGV 119 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 94 1290 Infinity Quaternary Pump User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done i
54. water and isopropanol 90 10 is a good choice for many applications 2 Put the solvent inlet tube into the solvent reservoir close it and connect the tube to the seal wash pump 3 Route the outlet of the wash tube into a waste container 1290 Infinity Quaternary Pump User Manual 45 3 Installing the Module Installation of Seal Wash Function 46 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 4 Using the Pump Leak and Waste Handling 48 Best Practices 49 Daily Weekly tasks 49 Power up Shut down the pump 49 Prepare the pump 50 How to deal with solvents 51 Select channels for Multi Channel Gradient Valve MCGV 51 Optional Inline Filter 52 Setting up the Pump with the Instrument Control Interface 53 Overview 53 Instrument Configuration 53 The Pump User Interface Dashboard Panel 55 Control Settings 58 Method Parameter Settings 60 Flushing the Filter 66 Solvent Information 68 Algae Growth in HPLC Systems 75 How to Prevent and or Reduce the Algae Problem 75 This chapter explains the operational parameters of the Agilent 1290 Infinity Quaternary Pump oh Agilent Technologies 41 4 Using the Pump Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing
55. 0 1840 Pin BNC Pin Agilent Signal Name module yoo la Shield Shield Analog pa o T Ne Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog _Se 3 Red Analog S 1290 Infinity Quaternary Pump User Manual 237 11 Identifying Cables Remote Cables 238 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1290 Infinity Quaternary Pump User Manual Identifying Cables Agilent Module to 3396 Series III 3395B Integrators 11 p n 03396 61010 Pin 33XX Pin A
56. 0 20020 Description Wrench open 1 4 5 16 inch Spanner double open ended 12X14 mm Chrome Torque wrench 2 25 Nm Bit Torx 10x25 mm Description Outlet valve primary pump head Internal gold seal for Outlet Valve Switch off pump at the main power switch Remove the front cover e Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Outlet Valve 1 Open the 2 5 mm hex screw at the top of the primary pump head which fixes the connection capillary of the heat exchanger Then lift up the capillary and remove it from the primary pump head 2 Agold seal between outlet valve and heat exchanger capillary is used for a tight connection The seal can be replaced separately as needed Hex screw l 3 Unscrew the outlet valve with a 14 mm wrench Gold seal 1290 Infinity Quaternary Pump User Manual 139 Maintenance Replacing the Outlet Valve 4 Insert the new outlet valve and tighten it using a torque wrench with a 14 mm bit set to 10 Nm 5 Insert the heat exchanger capillary into the outlet of the outlet valve Using a torque wrench with a 2 5 mm hex bit set 3 Nm and close the hex screw at the top of the outlet 6 Purge the system to remove air 140 1290 Infinity Quaterna
57. 04 between pump head and pressure sensor filters out particles which may be created in the pump by wear of piston or wash seals This filter can be replaced as required 66 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Flushing the Filter 3 An optional inline filter connected to the Multi Purpose Valve with a small pore size of about 0 3 um In Line Filter Assembly for 1290 Infinity Quaternary Pump 5067 5407 This filter can be flushed using the graphical user interface or replaced as required Inline filter Outlet filter In the instrument control panel of Agilent user interfaces use the context menu and select Flush Filter On see also Context Menu on page 57 1290 Infinity Quaternary Pump User Manual 67 4 Using the Pump Solvent Information Introduction Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 75 e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Materials in Flow Path Following materials are used in the flow pa
58. 13 ump drive stop not found 114 ump error 102 uaternary pump shutdown during analysis 105 reading of multi purpose valve tag failed 110 reading the pump encoder tag failed 105 O GT G GU TOUS 304 remote timeout 97 seal wash pump was missing when tried to turnon 119 shutdown 96 solvent counter exceeded limit 103 target pressure not reached for quaternary pump degasser 103 timeout wait for composition 115 timeout wait for flow 116 timeout wait for pressure 117 timeout wait for run volume 115 timeout wait for volume 116 timeout 96 unknown multi purpose valve type 112 valve hardware overcurrent MCGV 113 waste counter limit exceeded 104 writing the multi purpose valve tag failed 112 writing the pump encoder tag failed 106 extra column volume 80 F fan failed 101 filter flushing 66 firmware description 248 main system 248 resident system 248 update tool 249 updates 249 206 upgrade downgrade 206 fittings 130 42 214 flow rate limit exceeded 104 flow connections frequency range 25 G general error messages 96 handling acetonitrile 74 handling acids 74 handling buffers 73 heat exchanger replace 1 4 humidity 25 initialization mode selection 269 inlet valve stuck release 152 inlet valve replace 136 inlet weaver replace 134 inline filter install 198 remove 200 replacing parts 202 installation bench space 24 sealwash 45 site require
59. 3 14 15 p n 0515 1218 0905 1719 G4220 60016 5062 2484 0905 1718 G4220 63010 5067 5678 G4204 60022 64204 40006 G4220 60028 G4220 20028 G4220 20020 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Description Screw M5 40 mm long Pump Chamber Housing order pump head PE Seal Seal holder including backup ring Gasket seal wash pack of 6 Wash Seal PE Support Ring Seal Wash Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic Inlet Valve 1290 Infinity Quaternary Pump Fixation screw inlet weaver to PIV not shown Outlet valve primary pump head Headless screw for 1290 Infinity pump heads Internal gold seal for Outlet Valve 219 10 Parts and Materials Primary Pump Head Without Seal Wash Quaternary Pump Figure 23 Primary pump head Quaternary Pump without seal wash 220 1290 Infinity Quaternary Pump User Manual Item p n 1 8 6 0515 1218 2 1 3 1 0905 1719 4 1 G4220 60015 5 2 6 1 7 1 5067 5678 9 1 64204 60022 1 G4204 40006 10 1 G4220 60028 11 1 G4220 20028 12 1 64220 20020 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Description Screw M5 40 mm long Pump Chamber Housing order pump head PE Seal Support ring including backup ring Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic Inlet Valve 1290 Inf
60. 300 mm Pump to Autosampler SST cap 0 17 mm ID 600 mm pre swaged Inlet tubing Syringe with luerlock 5 mL Polypropylene Tubing grommet Frit 0 3 pm for inline filter 5 pk 1290 Infinity Quaternary Pump User Manual Others HPLC System Tool Kit Parts and Materials 10 The HPLC System Tool Kit G4203 68708 contains some accessories and tools needed for installation and repair of the module p n 1 0100 1681 1 0100 1710 1 01018 23702 1 5023 0240 1 8710 0060 2 8710 0510 1 8710 0641 1 8710 0899 1 8710 1534 1 8710 1924 1 8710 2392 1 8710 2393 1 8710 2394 1 8710 2409 1 8710 2411 1 8710 2412 1 8710 2438 1 8710 2509 1 8710 2594 1 9301 0411 1 9301 1337 1290 Infinity Quaternary Pump User Manual Description Adapter syringe seal wash tube Mounting Tool for Tubing Connections Insert tool Hex driver 4 slitted Hex key wrench 9 64 inch Wrench open 1 4 5 16 inch Hex key set 1 5 mm Pozidriv screwdriver Wrench 4 mm both ends open end Wrench open 14 mm Hex key 4 mm15 cm long T handle Hex key 1 5 mm straight handle 10 cm Hex key 9 64 inch 15 cm long T handle Wrench open end 5 16 3 8 inch Hex key 3 mm12 cm long Hex key 2 5 mm 15 cm long straight handle Hex key 2 0 mm Screwdriver Torx TX8 Double open end wrench 4 mm x 5 mm Syringe Plastic Adapter syringe solvent tube with fitting 231 10 Parts and Materials 1290 Infinity Pump Service Kit 1290 Infinity pump service kit 5067 4699
61. 5 General Safety Information 296 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 299 Radio Interference 300 Sound Emission 301 Agilent Technologies on Internet 302 8 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 1 Introduction Features 10 Overview of the Quaternary Pump 11 Operating Principle 12 Positions of the Multi Purpose Valve 14 System Overview 18 Leak and Waste Handling 18 This chapter gives an introduction to the module instrument overview and internal connectors Apg Agilent Technologies 1 Introduction Features 10 The G4204A Quaternary Pump is designed for highest performance GLP compliance and easy maintenance It includes the following features Seal wash function for continued high lifetime of pump seals for buffer applications Optional Jet Weaver for optimum mixing performance with a minimum of delay volume Automatic purge function for ease of use and unattended preparation of the system Auto tuning of the delivery cycle for compensation of elasticity and solvent volume change effects compressibility thermal expansion Solvent selection for optimum flow accuracy Fast defill function for improved intake and delivery performance Two pistons in series design for increased reliability High resolution piston movement control for smooth and reliable motion For specifications see Performance Specifications
62. 79 Replacing the Multi Purpose Valve 191 Replacing Parts of the Multi Purpose Valve 194 Replacing the Outlet Filter 196 Installing the Inline Filter 198 Removing the Inline Filter 200 Replacing Parts of the Inline Filter 202 Installing the Valve Rail Kit 205 Replacing Module Firmware 206 Preparing the Pump Module for Transport 207 10 Parts and Materials 211 Overview of Main Assemblies 212 Flow Connections 214 Seal Wash Function 215 Pump Head Assembly Parts 216 Primary Pump Head Parts 218 Secondary Pump Head Parts 222 Multi Purpose Valve 225 Solvent Cabinet 226 Cover Parts 228 Leak Parts 229 Accessory Kit 230 Others 231 1 Identifying Cables 233 Cable Overview 234 Analog cables 236 Remote Cables 238 BCD Cables 241 CAN LAN Cable 243 RS 232 Cable Kit 244 Agilent 1200 Module to Printer 245 1290 Infinity Quaternary Pump User Manual Contents 12 Hardware Information 247 Firmware Description 248 Electrical Connections 251 Interfaces 253 Setting the 8 bit Configuration Switch 260 Early Maintenance Feedback 263 Instrument Layout 264 13 LAN Configuration 265 What You Have To Do First 266 TCP IP Parameter Configuration 267 Configuration Switch 268 Initialization Mode Selection 269 Dynamic Host Configuration Protocol DHCP 273 Link Configuration Selection 276 Automatic configuration with Bootp 277 Manual Configuration 287 PC and User Interface Software Setup Setup 293 14 Appendix 29
63. AN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 17 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF manually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 276 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Automatic configuration with Bootp All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the detector configuration switch is set properly The setting should be either BootP or BootP amp Store see Table 13 on page 269 Assure that the det
64. AUTION valves and an outlet filter CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 9 Install the new pump head assembly by tightening the screws step by step Apply 5 Nm using a torque hex key which is included to the 1290 Infinity Service Kit p n 5067 4699 160 1290 Infinity Quaternary Pump User Manual Maintenance 9 CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 10 Insert the heat exchanger capillary into the outlet of the primary pump head Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 3 Nm and close the hex screw at the top of the outlet 11 Insert the new inlet weaver to the inlet valve 1 2 Fix the weaver with the plastic screw to the inlet valve 3 Connection to inlet weaver 1290 Infinity Quaternary Pump User Manual 161 9 Maintenance Replacing the Pump Head 12 Connect the capillary from the pressure sensor to the pump head outlet filter i Q Pressure sensor Outlet filter 162 1290 Infinity Quaternary Pump User Manual Maintenance 9 Disassembling the Pump Head
65. AUTION Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 24 1290 Infinity Quaternary Pump User Manual Site Requirements and Specifications 2 Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 15 2 kg 33 4 Ibs Dimensions 200 x 345 x 435 mm 8 x 13 5 x 17 inches height x width x depth Line voltage 100 240 V 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 180 W 615 BTU h Maximum Ambient operating 4 55 C 39 131 F temperature Ambient non operating 40 70 C 40 158 F temperature Humidity lt 95 r h at 40 C 104 F Non condensing Operating altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15092 ft For storing the module Safety standards Installation category II Pollution degree2 For indoor use only IEC CSA UL 1290 Infinity Quaternary Pump User Manual 25 2 Performance Specifications 26 Site Requirements and Specifications Table 2 Performance specifications Type Specification Comments Hydraulic system Settable flow range Flow prec
66. Browse to choose another location Click Next The default location for installation is C Program Files Agilent BootPService 10 Click Install to begin installation 1290 Infinity Quaternary Pump User Manual 279 13 LAN Configuration 11 Files load when finished the BootP Settings screen appears BootP Settings a BootP Tab File Create Tab File Edit BootP Addresses Logging BootP Log File ESS ad Sa REG Default Settings Subnet mask GO 0 0 0 Gateway GO 0 0 0 Cancel Help Figure 44 BootP Settings screen 12 In the Default Settings part of the screen if known you can enter the subnet mask and gateway Defaults can be used The default subnet mask is 255 255 255 0 The default gateway is 192 168 254 11 13 On the BootP Settings screen click OK The Agilent BootP Service Setup screen indicates completion 14 Click Finish to exit the Agilent BootP Service Setup screen 15 Remove the DVD from the drive This completes installation 16 Start BootP Service in the Windows services On the Windows desktop click right on Computer icon select Manage gt Services and Applications gt Services Select the Agilent BootP Service and click Start 280 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address select the Do you want
67. Error ID 29235 Insufficient power of drive encoder LED Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual 111 7 Error Information Drive encoder error Error ID 29237 29238 29239 29215 An error has occurred for the pump drive encoder Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Writing the multi purpose valve tag failed Error ID 29241 Writing the multi purpose valve tag failed Parameter None Probable cause Suggested actions 1 Multi purpose valve head tag defect Replace multi purpose valve head 2 Multi purpose valve tag head reader is Please contact your Agilent service defect representative Unknown multi purpose valve type Error ID 29242 The type information of the multi purpose valve is invalid e Parameter None Probable cause Suggested actions 1 Wrong valve head installed Check or replace multi purpose valve head 2 Valve head has invalid RFID tag content Check or replace multi purpose valve head 112 1290 Infinity Quaternary Pump User Manual Error Information 7 Pump drive encoder error Error ID 29211 The pump drive encoder has generated no signal Parameter 1 2 referring to pump drive Probable cause Suggested ac
68. IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 39 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 40 Using Stored Principle Using Default When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 14 on page 271 Default Active Parameter Parameter Figure 41 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Table 14 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0
69. Lamp Tag Use UV lamp anyway Use cell anyway OV 1 output range OV 1V_ output range LAN Subnet Mask Stays off at power on 255 255 248 0 LAN Def Gateway 134 40 24 1 System I Controller DAD I Instant Pilot LAN Configuration Figure 56 5 Press the Edit button only visible if not in Edit mode perform the required changes and press the Done button 6 Leave the screen by clicking Exit 292 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 PC and User Interface Software Setup Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see also Initialization Mode Selection on page 269 FIXED IP address Local Area Connection Properties 2 x Internet Protocol General General Connect using You can get IP setings assigned automatically if your network supports this capability Otiierwise you need to ask your network administrator for the appropriate IPiisettings a Xircom CreditCard Ethemet 10 100 Modem 56 C_Dbierrarrtatiireseautomatically Use the following IP address IP adiere 192 168 254 1 Subnet mask 255 255 248 0 Default gateway p Cc Install Uninstall Properties Ji ER Use the folo General Transmission Control Protocol Intemet Protocol The default pEen
70. Manual Maintenance 9 Replacing the Heat Exchanger 1 Remove the 19 mm screw at the front of the secondary pump head 2 Remove the front plate 3 Remove the screw at the bottom of the pump head Do not drop the golden spacer fitting Screw 1290 Infinity Quaternary Pump User Manual 175 9 Maintenance Replacing the Heat Exchanger 4 Lift out the heat exchanger 5 If removed first insert the spacer fitting Then insert the new heat exchanger to the opening in the pump head and lift it over the pins Spacer fitting 6 Use the 19 mm screw for fixing the front plate Front plate 7 Insert and fix the screw Screw 176 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing Wash Seal and Gasket Tools required p n Description 01018 23702 Insert tool Parts required p n Description 0905 1718 Wash Seal PE 5062 2484 Gasket seal wash pack of 6 1 Use the steel side of the insert tool for removing the wash seal Wash seal D 1290 Infinity Quaternary Pump User Manual 177 9 Maintenance 2 Use the soft plastic side of the insert tool for inserting the 3 Put the gasket into the support ring and insert the seal wash seal into the support ring Wet the wash seal with holder isopropanol or another suitable solvent before insertion B Seal holder with backup ring Insert tool Gasket Wash seal Support ring
71. OFF Using Stored OFF ON ON Using Default ON OFF OFF DHCP 1 Requires firmware B 06 40 or above Modules without LAN on board see G1369C LAN Interface Card Bootp When the initialization mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp Server Figure 38 Bootp Principle 1290 Infinity Quaternary Pump User Manual 269 13 LAN Configuration 270 Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP
72. PP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set 1290 Infinity Quaternary Pump User Manual 249 12 Hardware Information Main FW update Resident System Main System Resident FW Update Figure 31 Firmware Update Mechanism Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tool
73. Type Test 1290 Infinity Quaternary Pump User Manual 301 14 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 302 1290 Infinity Quaternary Pump User Manual Index 8 bit configuration switch on board LAN 260 A Agilent Lab Advisor software 92 Agilent Lab Advisor 92 Agilent oninternet 302 User Interface Software Setup 293 ambient non operating temperature 25 ambient operating temperature 25 analog signal 25 7 analog cable 236 apg remote 258 assembling pump head 179 automatic configuration with Bootp 277 BCD cable 241 bench space 24 BootP service installation 279 restart 286 settings 286 stop 285 Bootp amp Store 270 automatic configuration 277 initialization modes 269 using default 271 using stored 271 c cable analog 236 BCD 241 CAN 243 LAN 243 remote 238 RS 232 244 cables analog 234 BCD 234 CAN 235 LAN 235 overview 234 remote 234 RS 232 235 CAN cable 243 capillaries 130 cleaning 129 compensation sensor open 99 compensation sensor short 100 condensation 24 configuration switch 268 configuration one stack 31 two stack front 34 two stack rear 35 two stack 34 cover parts 228 1290 Infinity Quaternary Pump User Manual Index D defect connection between main board and pump drive encoder 109 defect on arrival 30 degasser when to use 78 degasser s
74. Using the Pump Handling of Acetonitrile Acetonitrile is a solvent that is frequently used in reversed phase chromatography Despite of its common use it can be a source of issues if not handled correctly Acetonitrile degrades through polymerization and such polymers can stick to surfaces in LC systems and e g cause issues with valve performance and therefore affect retention time precision Polymers can also show up as background noise in MS detectors When using acetonitrile e Use high quality solvents from renowned suppliers e Use fresh solvents and filter them e Minimize exposure to light and air oxygen e Choose a bottle size which fits to your application and usage e Acids accelerate polymerization If possible avoid such additives or refresh solvents more frequently e Pure acetonitrile polymerizes faster If your application allows add about 5 water and adjust gradient compositions e Do not leave acetonitrile in unused systems to avoid aging If not in use flush all solvent lines with a mixture of water and 10 isopropanol In case of blocked valves flush the system with hot water Knock at valves flush them see Releasing a Stuck Inlet Valve on page 152 or ultrasonicate them e g in methanol Handling of Acids Acids can corrode stainless steel and other materials in the flow path of LC systems For stainless steel the minimum pH is 2 3 for corrosive acids and pH 1 for non corrosive acids Pl
75. a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if n
76. absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1290 Infinity Quaternary Pump User Manual 23 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 25 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation C
77. ainst damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING ahaa alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 296 1290 Infinity Quaternary Pump User Manual Appendix 14 General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual
78. aintenance 9 Preparing the Pump Module for Transport When If the module shall be transported or shipped Parts required p n Description 9301 0411 Syringe Plastic 9301 1337 Syringe adapter G4204 44000 Transport protection foam CAUTION Mechanical damage gt For shipping the module insert the Protective Foam to protected the module from mechanical damage gt Be careful not to damage tubing or capillary connections while inserting the module in the Protective Foam _ Flush all solvent channels with isopropanol 2 Remove solvent inlet tubes from solvent reservoirs and tubing clips at other modules 3 Remove tubings between the seal wash function and solvent bottle waste 4 Remove cable and capillary connections to other modules 5 Remove the module from the stack 6 Remove the waste tube 1290 Infinity Quaternary Pump User Manual 207 208 Maintenance Preparing the Pump Module for Transport 7 Disconnect the degasser outlet tubings at the MCGV one after another Use a syringe for removing liquid from the degasser and the solvent tubings y Fd MAN EINN Uc 8 Reconnect the degasser outlet tubings to the MCGV Remove the degasser inlet tubings i lt n ee 1290 Infinity Quaternary Pump User Manual Maintenance 9 Preparing the Pump Module for Transport 9 You may keep internal tubing and capillary connections Degasser A A gt e manatee Sx Pump heads W ANS
79. ameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive spindle is defect Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual Error Information 7 Timeout Wait for Composition Error ID 29180 A target condition composition has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes Timeout Wait for run volume Error ID 29181 A target condition run volume which is the volume delivered since the method run start has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly for example the flow rate Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes 1290 Infinity Quaternary Pump User Manual 115 7 Error Information Timeout Wait for Volume Error ID 29182 A target condition volume which is the delivered flow since the limi
80. an break it 172 7 To flush out particles from the pump head use pressurized air 1 2 and 3 Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection 1290 Infinity Quaternary Pump User Manual Maintenance 9 8 To remove wear use isopropanol 10 To dry the pump head and to remove residues use 9 To clean the piston and the pump head surface use the pressurized air 1 2 and 3 ultra fine abrasive mesh p n 8660 0852 11 If the wash seal shall be replaced by a new one use the steel side of the insert tool for removing it Wash seal 1290 Infinity Quaternary Pump User Manual 173 9 Maintenance Replacing the Heat Exchanger Tools required Parts required Preparations CAUTION p n 5023 2501 p n G4220 81013 G4220 20028 G4220 20001 Description Wrench 19 mm Screwdriver Torx T10 Description Heat Exchanger secondary pump head only Headless screw for 1290 Infinity pump heads Spacer Fitting Remove the pump head assembly from the pump Remove the secondary pump head from the link plate Loss of small spacer fitting Inside the secondary pump head is a small spacer fitting which can be dropped easily when removing the heat exchanger gt The heat exchanger does not need to be removed for pump head maintenance 174 1290 Infinity Quaternary Pump User
81. annel Gradient Valve MCGV 7 Tighten the two screws 1 2 and connect the cable to its 6 Place the new MCGV into position connector 3 Channel A NOTE Make sure that channel A of the MCGV is put at the bottom right position 150 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Multi Channel Gradient Valve MCGV 8 Install the MCGV cover 9 Reconnect the waste funnel with the waste tube holder in the top cover Insert waste tube in the holder in the leak pan and clip tube to the MCGV cover holder Pe Waste unnel Next Steps 10 Install the inlet weaver see Replacing the Inlet Weaver on page 134 11 Reconnect solvent tubes for channels A D from the MCGV to the degasser outlets 1290 Infinity Quaternary Pump User Manual 151 9 Maintenance Releasing a Stuck Inlet Valve Tools required p n Description 9301 0411 Syringe Plastic 0100 1681 Syringe adapter luer barb 0100 1710 Mounting Tool for Tubing Connections Beaker CAUTION Pressure damages the multi channel gradient valve MCGV and or degasser gt Never apply pressure to the MCGV or degasser gt Directly connect the syringe to the inlet weaver 1 Remove tubing connections channels A B C and D to the MCGV such that you can access the inlet weaver 152 1290 Infinity Qua
82. anual changes 2 Leak Run system pressure test for identifying and localizing the leak Tighten leak Drive Encoder failed Error ID 29210 Drive encoder failed during pump drive calibration Probable cause Suggested actions 1 Internal error Contact Agilent support 1290 Infinity Quaternary Pump User Manual 117 Error Information Error ID 29216 Drive phases differ too much in electric resistance Pump drive calibration has failed due to a strong difference electric resistances for different motor phases Probable cause 1 Pump drive cable defect 2 Pump drive defect 3 Defective main board Degasser s pressure limit violation Error ID 29220 Pressure too far above the limit Probable cause 1 Leak in degasser chamber or degasser tubing 2 Defect vacuum pump 3 Degasser chamber empty or connected to air Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Block unused degasser channels 1290 Infinity Quaternary Pump User Manual Error Information 7 Seal wash pump was missing when tried to turn on Error ID 29223 The seal wash pump has not been detected while being configured or detected before Probable cause Suggested actions
83. ards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 1290 Infinity Quaternary Pump User Manual 127 9 Maintenance Overview of Maintenance The following pages describe maintenance simple repairs of the module that can be carried out without opening the main cover 128 1290 Infinity Quaternary Pump User Manual Maintenance 9 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 1290 Infinity Quaternary Pump User Manual 129 9 Maintenance Installing Fittings and Capillaries Solvent can spray under high pressure gt Observe appropriate safety procedures for example goggles safety gloves and protective clothing when opening flow path CAUTION Deformation of fittings and seals Liquid drops under high pressure up to 1200 bar act like solid parts Tightening connections under high pressure can deform or destroy fittings and seals gt Never tighten flow connections under pressure
84. art see Special Settings on page 262 Then try a firmware update see Replacing Module Firmware on page 206 If this does not help a main board replacement is required 1290 Infinity Quaternary Pump User Manual Troubleshooting and Diagnostics 6 Available Tests vs User Interfaces Depending on the user interface the available tests and the screens reports may vary see chapter Test Functions and Calibrations e Preferred tool should be the Agilent Lab Advisor software see Agilent Lab Advisor Software on page 92 e The Agilent ChemStation may not include any maintenance test functions e Screenshots used within these procedures are based on the Agilent Lab Advisor software 1290 Infinity Quaternary Pump User Manual 91 Troubleshooting and Diagnostics Agilent Lab Advisor Software 92 The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status Early Maintenance Feedback counters EMF instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon request the user can send this report to Agilent for a significantly improved troubleshooting and repair process The Agilent Lab Advisor so
85. ash p n 0515 1218 G4220 20003 G4220 81013 G4220 20028 G4220 20001 0905 1719 G4220 60015 5067 5678 G4204 60004 Description Screw M5 40 mm long Pump Head Screw Pump Head Front Plate order pump head Heat Exchanger secondary pump head only Headless screw for 1290 Infinity pump heads Spacer Fitting Pump Chamber Housing order pump head PE Seal Support ring including backup ring Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic Outlet filter 1290 Infinity Quaternary Pump 1290 Infinity Quaternary Pump User Manual Multi Purpose Valve Parts and Materials 10 Figure 26 Item 1 1 3 2 1 3 1 4 1 5 1 6 1 Multi purpose valve parts p n 5067 4174 1535 4857 5068 0001 5068 0120 5068 0123 1535 4045 5068 0106 1290 Infinity Quaternary Pump User Manual Description Multi Purpose Valve Head Stator screws 10 Pk Stator head Stator ring Rotor seal Multi Purpose Valve 1290 Infinity Quaternary Pump 1200 bar Bearing ring Spanner nut 225 10 Parts and Materials Solvent Cabinet Solvent Cabinet Figure 27 Solvent Cabinet Parts 1 Figure 28 Solvent Cabinet Parts 2 226 1290 Infinity Quaternary Pump User Manual Item p n 1 5065 9981 5043 0207 3 5065 9954 4 5042 8907 5 9301 1450 6 9301 1420 7 G4220 60007 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Description Solvent cabine
86. ay volume in a system includes the volume in the pump from the point of mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column For the 1290 Infinity Quaternary Pump all pump parts downstream the MCGV contribute to the delay volume i e inlet weaver pump heads capillary connections filters and the optional Jet Weaver 80 1290 Infinity Quaternary Pump User Manual How to Optimize the Performance of Your Module 5 How to Configure the Optimum Delay Volume The design of the 1290 Infinity Quaternary Pump offers a strongly decreased delay volume compared to standard 600 bar pressure pumps For the 1290 Infinity Quaternary Pump mixing is done in the multi channel gradient valve at ambient pressure As all pump parts in the flow path after mixing contribute to the delay volume this includes also pump heads of the quaternary pump flow connections filters mixers etc Therefore the delay volume of a quaternary pump is by design larger than that of a binary pump All listed components including the inlet weaver and pump heads ensure a good mixing performance resulting in excellent composition precision and accuracy highly reproducible retention times and low baseline noise This ensures best results for most applications Per default the 1290 Infinity Quaternary Pump does not require and include a Jet Weaver as solvents are mixed in the MCGV and mixin
87. ays be included to the flow path 78 1290 Infinity Quaternary Pump User Manual How to Optimize the Performance of Your Module 5 Operational Hints for the Multi Channel Gradient Valve MCGV In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations However in the mixing point of the gradient valve at the boundary between the two solvents micro precipitation is possible Gravity forces the salt particles to fall down Normally the A channel of the valve is used for the aqueous salt solution and the B channel of the pump is used for the organic solvent If used in this configuration the salt will fall back into the aqueous solution and will be dissolved When using the pump in a different configuration e g D salt solution A organic solvent the salt can fall into the port of the organic solvent and may lead to performance problems When using salt solutions and organic solvents it is recommended to connect the salt solution to one of the bottom ports of the MCGV and the organic solvent to one of the upper gradient valve ports It is best to have the organic channel directly above the salt solution channel Regular flushing with water of all MCGV channels is recommended to remove all possible salt deposits in the valve ports Precipitations formed during the mixing of buffers and organic solvents which do not dissolve salts may cause a loss of pump
88. cally with no further manual configuration necessary The Quaternary Pump configuration parameters are in two sections Communication Options Communication The parameters in this dialog box are detected automatically during autoconfiguration Device name Type ID Serial number Firmware revision Button Connection settings 1290 Infinity Quaternary Pump User Manual 53 4 Using the Pump Options Pressure Unit select the pressure units from the drop down list bar psi or MPa Seal wash installed This check box is marked to indicate that an optional seal wash has been detected during autoconfiguration Installed mixer The installed mixer is detected during autoconfiguration For manual configuration click the down arrow and select the installed mixer from the list or choose No mixer installed Configure Solvent Type Catalogs Displays the Solvent Type Catalogs dialog box which allows you to import and export solvent calibration data See Importing Solvent Calibration Tables on page 86 Please refer to the online help of your user interface for more detailed information 54 1290 Infinity Quaternary Pump User Manual Using the Pump 4 The Pump User Interface Dashboard Panel Module Graphic The items in the pump graphic have the following meaning and function The level of solvent in the bottle is denoted by the green area when the solvent level falls below the specified vo
89. ceiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces The module includes a DC Out 24 VDC power line that is intended to be used with certain modules that operate as CAN slaves for example external valves The line has a limited output of 1 7 A and is self resetting 1290 Infinity Quaternary Pump User Manual 259 12 Hardware Information Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e Default is ALL switches DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity
90. ched on again does not need to be re initialized Seal Wash The seal wash can be set up to be run once Single wash or periodically Periodic Off no seal wash is used Single wash the seal will be purged for a specified time Periodic a periodic wash will be applied for a defined period in minutes The option is available only when the pump has seal wash capability The seal wash capability is detected by the module itself If seal wash is installed it is recommended to use it in order to increase the primary seal lifetime Seal Wash Run Allows you to define when to use the seal wash Mode Off The seal wash is inactive On when pump is on The seal wash is active only when the pump is on On all the time The seal wash is active when the pump is on or in standby mode Automatic Turn On Module can be turned on at a specified date time This feature can only be used if the module power switch is turned on 58 1290 Infinity Quaternary Pump User Manual Table 3 Pump control parameters Using the Pump 4 Parameter Limits Description Purge Time 0 100 00 min Setup and activation of Purge parameters The automatic purge valve can in steps of 0 01 be used for purging the system The process has been automated for ease Flow 0 000 of use 5 000 mL min for Off Turns off the purge each channel in steps of 0 001 Prime Conditioning at least 200 bar gt 500 bar is better On The device
91. crystals Always use solvent inlet filters Avoid conditions where mixing of buffers and organic solvents may cause precipitation as this impairs the reproducibility of chromatographic experiments and may also reduce the system life time For example in reversed phase chromatography avoid buffers especially phosphate buffers with a concentration higher than 20 mmol L For phosphate buffers avoid compositions containing more than 65 acetonitrile or other organic solvents Use a minimum flow rate of 5 uL min or 1 composition per solvent channel whatever is greater to avoid cross flow Cross flow can be caused by micro leaks in pump heads and can result in buffer precipitation in pump heads channel blocking or reduced pump head life time through wear of seals and pistons When installing tubing connections to the MCGV use lower channels A D for aqueous solvents and upper channels for organic solvents This will re dissolve precipitates more easily Never leave buffers in a system without flow Before shutting down a system flush it extensively with warm water to avoid clogging of valves capillaries or flow cells or reducing the life time of your column If the system is not used for some time for example more than a day depending on lab temperature fill all solvent lines with organic solvent or water with at least 10 isopropanol Regularly maintain the LC system 1290 Infinity Quaternary Pump User Manual 73 4 74
92. d on page 155 Both pump drives must be retracted Correct 188 1290 Infinity Quaternary Pump User Manual Maintenance 9 Assembling the Pump Head CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 18 Install the new pump head assembly by tightening the screws step by step Apply 5 Nm using a torque hex key which is included to the 1290 Infinity Service Kit p n 5067 4699 CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 19 Insert the heat exchanger capillary into the outlet of the primary pump head Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 3 Nm and close the hex screw at the top of the outlet 1290 Infinity Quaternary Pump User Manual 189 9 Maintenance Assembling the Pump Head 20 Insert the new inlet weaver to the inlet valve 1 2 Fix the weaver with the plastic screw to the inlet valve 3 Connection to inlet weaver 190 21 Connect the capillary from the pressure sensor to the pump head outlet filter ie AN ade Pressure sensor Outlet filter 1290 Infinity Quaternary Pum
93. ded to protect the downstream system from blockages e for solvent combinations that can form precipitation after mixing for applications running with buffers General hints for effective usage of the inline filter e filter solvents before usage follow best practices back flush the filter weekly e exchange the filter frit on a regular basis Damage to the valve gt Use the filter flush mode only if the optional inline filter is installed 52 See Technote G4226 90131 for further reference 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Setting up the Pump with the Instrument Control Interface Overview Parameters described in following sections is offered by the instrument control interface and can usually be accessed through Agilent instrument control software For details please refer to manuals and online help of respective user interfaces Instrument Configuration Use the Instrument Configuration dialog box to examine and if necessary modify your instrument configuration The Configurable Modules panel contains a list of all modules available for configuration The Selected Modules panel contains the list of configured modules Auto Configuration Under Communication settings select either the Host Name option or the IP address option and enter the appropriate value for the host computer to enable automatic detection of the hardware configuration The system configures the instrument automati
94. dicate the operational state of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations Diagnostic Signals The module has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems These can be added like normal signals in the Agilent ChemStation software 88 1290 Infinity Quaternary Pump User Manual Troubleshooting and Diagnostics 6 Status indicators Status indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator Power switch L4H W x Serial number Figure 16 Location of status indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1290 Infinity Q
95. e Description Agilent module to 3396 integrators Agilent module to general purpose 1290 Infinity Quaternary Pump User Manual CAN cables p n 5181 1516 5181 1519 LAN cables p n 5023 0203 5023 0202 RS 232 cables p n G1530 60600 RS232 61601 5181 1561 Identifying Cables 11 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m 1290 Infinity Quaternary Pump User Manual 235 11 Identifying Cables Analog cables T O One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog 236 1290 Infinity Quaternary Pump User Manual Identifying Cables 11 Agilent Module to BNC Connector p n 812
96. e Disassembling the Secondary Pump Head VTE yy Damage of pump piston The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 Remove the outlet filter at the top of the pump head 170 1290 Infinity Quaternary Pump User Manual Maintenance 9 2 For disassembling the pump head remove the 4 hex screws at the rear of the pump head 3 Remove the front part of the pump head including pump chamber housing with pump seal and seal holder If the seal wash option is installed also remove the support ring with wash seal and gasket Support ring with wash seal Seal wash gasket Seal holder including backup ring Pump chamber housing 4 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 5 Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals 1290 Infinity Quaternary Pump User Manual 171 9 Maintenance 6 Use the steel side of the insert tool for removing the pump seal from the pump chamber housing AT Insert tool Pump seal Do not use the pump piston for that purpose as this c
97. e contact your Agilent service representative Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 1290 Infinity Quaternary Pump User Manual 101 7 Error Information Pump Error Messages These errors are pump specific Pressure of quaternary pump above upper limit Error ID 29163 The pressure has exceeded the upper pressure limit e Parameter Measured pressure Probable cause Suggested actions 1 Blockage in flow path after the pressure Check for blockages in the LC system e g sensor purge valve Jet Weaver degraded column column frits needle needle seat capillaries etc Check for particles in the solvent 2 Inappropriate settings pressure limit flow Decrease flow rate rate Increase pressure limit Pressure below lower limit Error ID 29176 The pressure has dropped below the lower limit Parameter None Probable cause Suggested actions 1 Leak Check for leaks 2 Bottle empty Check bottle filling 3
98. e next as the solvent flows into the next module s leak funnel 8 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to waste The condense drain outlet of the autosampler cooler 5 guides condensate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container 1290 Infinity Quaternary Pump User Manual 19 1 20 Introduction System Overview 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 22 Physical Specifications 25 Performance Specifications 26 This chapter provides information on environmental requirements physical and performance specifications ott Agilent Technologies 21 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 25 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuse
99. ease note that for non volatile acids like phosphoric acid or perchloric acid concentrations increase after evaporation of water This means that originally diluted acids can damage parts over time e g because of liquid which has left the solvent path through micro leaks Such systems should be flushed regularly with pure water and may require shorter maintenance cycles Using the seal wash function should be considered for protecting pump heads 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure e Column filters to plug giving high syste
100. ector connected to the network is powered off If the Agilent BootP Service program is not already installed on your PC then install it from your Agilent ChemStation DVD located in folder BootP About Agilent BootP Service The Agilent BootP Service is used to assign the LAN Interface with an IP address The Agilent BootP Service is provided on the ChemStation DVD The Agilent BootP Service is installed on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN The BootP service must be running TCP IP network protocol and cannot run a DHCP server 1290 Infinity Quaternary Pump User Manual 277 13 LAN Configuration 278 How BootP Service Works When an instrument is powered on an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides its hardware MAC address as an identifier The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument The instrument receives its IP address and host name and maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent BootP Service must be running every time the instrument powers up If the Agilent BootP Service runs in the background the instrument will receive its IP address on power up The Agilent LAN Interface can
101. ed actions 1 Blockage in flow path Remove capillary connection to system check outlet filter check valves check pump head 2 Blockage of pump drive Drive motor defect Please contact your Agilent service representative Overcurrent of pump drive Error ID 29202 The current consumption of the pump drive is too high e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit Multi Purpose Valve heat exchanger 2 Drive motor defect Please contact your Agilent service representative 108 1290 Infinity Quaternary Pump User Manual Error Information 7 Deliver underrun Error ID 29233 Internal error e Parameter None Probable cause Suggested actions 1 Internal error Please contact your Agilent service representative 2 Firmware issue Use a minimum firmware revision of B 06 55 Defect connection between main board and pump drive encoder Error ID 29208 Defect connection between main board and pump drive encoder e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Defect connection between main board and Please contact your Agilent service pump drive encoder representative 2 Defect encoder Please contact your Agilent service representative Pump drive encoder defect Error ID 29209 Defect pump drive encoder e Parameter 1 2 referring to pump drive
102. eel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens e High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCl Oo 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbo
103. een 4 In the Edit BootP Addresses screen press Add to create a new entry or select an existing line from the table and press Modify or Delete to change the IP address comment subnet mask for example in the Tab File If you change the IP address it will be necessary to power cycle the instrument for the changes to take effect 5 Leave Edit BootP Addresses by pressing Close 6 Exit BootP Settings by pressing OK Restarting the Agilent BootP Service 1 In the Windows control panel select Administrative Tools gt Services The Services screen appears see Figure 47 on page 285 2 Right click Agilent BootP Service and select Start 3 Close the Services and Administrative Tools screens 286 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 49 Manual Configuration Principle 1290 Infinity Quaternary Pump User Manual 287 13 LAN Configuration With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters s
104. eposit on pistons and seals The seal wash function regularly cleans these parts automatically Contaminated seal wash solvent gt Do not recycle seal wash solvent to avoid contamination gt Weekly exchange seal wash solvent 50 1290 Infinity Quaternary Pump User Manual Using the Pump 4 How to deal with solvents e Use clean bottles only e Exchange water based solvents daily Select solvent volume to be used up within 1 2 days Use only HPLC grade solvents and water filtered through 0 2 um filters e Label bottles correctly with bottle content and filling date expiry date Use solvent inlet filters Reduce risk of algae growth use brown bottles for aqueous solvents avoid direct sunlight Select channels for Multi Channel Gradient Valve MCGV Use lower channels A and or D for buffer solutions e Regularly flush all MCGV channels with water to remove possible salt deposits e Check compatibility of buffers and organic solvents to avoid precipitation 1290 Infinity Quaternary Pump User Manual 51 4 Using the Pump Optional Inline Filter CAUTION The pump can be equipped with an additional inline filter In Line Filter Assembly for 1290 Infinity Quaternary Pump 5067 5407 with a nominal filter pore size of 0 3 uL Advantages of the inline filter Very small internal volume Specified for working at high pressures e Possibility of back flushing the filter Using the inline filter is recommen
105. er Manual Using the Pump 4 Best Practices Daily Weekly tasks Daily tasks e Replace mobile phase based on water buffer e Replace organic mobile phase latest every 2nd day e Check seal wash solvent e Run conditioning with composition of your application Weekly tasks e Change seal wash solvent 10 90 isopropanol water and bottle e Flush all channels with water to remove salt deposits Visually inspect solvent filters Clean or exchange if necessary Power up Shut down the pump Power up the pump e Use new or different mobile phase as required e Purge pump heads with 2 5 3 mL min for 5 min Condition pump heads for 10 20 min Long term shut down of the pump Flush system with water to remove buffer Use recommended solvents to store the system e Power off the pump or system 1290 Infinity Quaternary Pump User Manual 49 4 Using the Pump Prepare CAUTION the pump Purge Use the Purge function to e fill the pump exchange a solvent remove air bubbles in tubes and pump heads Condition Use the Conditioning function e daily when starting the pump to minimize pressure ripple by dissolving air bubbles in pump heads Condition your complete system with solvents and composition of your application for example 50 50 A B at 0 5 mL min Seal wash Using the seal wash function is recommended when using buffers or other non volatile solvents or additives that could d
106. ered ONS You can get IP settings assigned automatically if your network supports ie ne cali Dre is De ies connie Altemate DNS this capability Otherwise you need to ask your network administrator for the appropriate IP settings Show icon in taskbar when connected Obtain an IP address automatically C Demeter scares automatic IP address via DHCP tli I requires special IT setup of the network Obtain DNS server address automatically C Use the following DNS server addresses Figure 57 Changing the TCP IP settings of the PC 1290 Infinity Quaternary Pump User Manual 293 13 LAN Configuration User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide 294 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 14 Appendix General Safety Information 296 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 299 Radio Interference 300 Sound Emission 301 Agilent Technologies on Internet 302 This chapter provides additional information on safety legal and web Ee Agilent Technologies 295 14 Appendix General Safety Information Safety Symbols Table 19 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus ag
107. eries Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed 254 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Table 6 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 2 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as
108. et by any method the parameters may be altered by opening a Telnet session 1 2 288 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK Type the following at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt E C AWINDOWS system32 cmd _exe C gt telnet 134 46 27 95 Figure 50 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switch on page 268 When the connection was established successfully the module responds with the following ET C AWINDOWS system32 cmd exe telnet 134 40 27 95 Agilent Technologies G1i315C PP H000024 Figure 51 A connection to the module is made 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 3 Type and press enter to see the available commands WINDOWS system32 cmd_exe telnet 134 40 27 95 Agilent Technologies G1315C PP 8880624 gt command syntax description display help info display current LAN settings ip lt x x x x gt set IP Address sm X X X X gt set Subnet Mask gu lt x x x x gt set Default Gateway exit exit shell gt Figure 52 Telnet Commands Table 18 Telnet Commands Value Description displays syntax and descriptions of commands displays cu
109. ew 1290 Infinity Quaternary Pump User Manual 3 33 3 Installing the Module Optimizing the Stack Configuration Two Stack Configuration In case the autosampler thermostat is added to the system a two stack configuration is recommended which places both heavy modules 1290 Infinity pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 9 on page 34 and Figure 10 on page 35 nstant Pilot Detector Column compartment 0 0000 0000 0000 Solvent cabinet Autosampler ermostat for the ALS optional Figure 9 Recommended two stack configuration for 1290 Infinity with quaternary pump front view 34 1290 Infinity Quaternary Pump User Manual Installing the Module 3 Optimizing the Stack Configuration LAN to control software AN Bus cable to nstant Pilot Analog getector signal mermo pable HAN AN UN AN CAN Bus cable AC Power Figure 10 Recommended two stack configuration for 1290 Infinity with quaternary pump rear view 1290 Infinity Quaternary Pump User Manual 35 3 Installing the Module Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling It is important that all
110. for RS 232 e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode For normal operation use the default best settings SX Agilent Tecdnsiogies VUeEUUUYUYUY 260 Figure 34 Location of Configuration Switch example shows a G4212A DAD 1290 Infinity Quaternary Pump User Manual Hardware Information 12 To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Table 9 8 bit Configuration Switch with on board LAN Mode Function sw1 sw2 sw3 sw4 sw5 swe sw7 sws LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 xX x x X xX 10 MBit half duplex 1 0 0 x xX X 10 MBit full duplex 1 0 1 x xX xX 100 MBit half duplex 1 1 0 x X X 100 MBit full duplex 1 1 1 x x x Bootp x x x 0 0 0 Bootp amp Store x x x 0 0 1 Using Stored x x x 0 1 0 DHCP x xX x 1 0 0 Using Default x X Xx 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 Xx Revert to Default Data Coldstart x X x 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 262 1290 Infinity Quaternary Pump User Manual 261 12 Ha
111. ftware is available in two versions e Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 7 Error Information What Are ErrorMessages 95 General Error Messages 96 Timeout 96 Shutdown 96 Remote Timeout 97 Lost CAN Partner 98 Leak Sensor Short 98 Leak Sensor Open 99 Com
112. g is further improved in the inlet weaver pump heads and subsequent parts in the flow path Therefore no Jet Weaver is required for most applications The V380 Jet Weaver high performance mixer is optionally available for demanding applications which use solvents in different channels for example A versus B that differ strongly in their UV Vis absorption for example by using trifluoroacetic acid TFA as a modifier which has a high absorbance Solvent packages created by the pump may persist until the solvent reaches the detector flow cell Absorption fluctuations can then show up as baseline noise also referred to as mixing noise Applications like impurity quantitation or lowest level compound detection require minimizing this noise The V380 Jet Weaver strongly improves mixing and therefore reduces baseline noise and improves sensitivity in detection Patented Agilent microfluidic technology offers high mixing performance at a low internal volume of 380 uL which is the physical volume of all channels It contributes with 150 uL to the pump delay volume lt 350 uL without Jet Weaver which is the partial mixer volume that creates a composition change corresponding to the delay volume 1290 Infinity Quaternary Pump User Manual 81 5 Howto Optimize the Performance of Your Module How to Configure the Optimum Delay Volume Figure 15 The Jet Weaver mixer The installation procedure is illustrated in Installing the Jet Weaver on
113. gilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 i 3 3 Gray Start Low z NC 4 Blue Shut down Low e an NC 5 Pink Not is connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected 1290 Infinity Quaternary Pump User Manual 239 11 Identifying Cables Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL White 1 Digital ground A Q 1 ou Brown 2 Prepare run Low 00 pee DE KEY Gray 3 Start Low oo o0 S EE Blue 4 Shut down Low ug Pink 5 Not og connected 15 o Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 240 1290 Infinity Quaternary Pump User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end
114. gs MAC Addre 838D38A8838 stored TCP IP settings in non volatile memory sing Stored TCP IP Properties active i IP Address 134 40 27 95 connected to PC with controller software e g Agilent Subnet Mask 255 255 248 0 ChemStation here not connected Def Gateway 134 409 24 1 stored IP Address gt 134 40 27 99 z 255 255 248 0 Gateway 134 40 24 1 Controllers z no connections Figure 54 Telnet Change IP settings 290 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 7 When you have finished typing the configuration parameters type exit and press Enter to exit with storing parameters cx WINDOWS system32 cmd exe Agilent Technologies G4296A DEGHBHQH06 gt exit Connection to host lost C V a Figure 55 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 134 40 27 99 1290 Infinity Quaternary Pump User Manual 291 13 LAN Configuration With the Instant Pilot G4208A To configure the TCP IP parameters before connecting the module to the network the Instant Pilot G4208A can be used 1 From the Welcome screen press the More button 2 Select Configure 3 Press the DAD button 4 Scroll down to the LAN settings 7 Configure DAD lt Not Set gt Temperature Control ON UV
115. guration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Table 7 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 33 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1290 Infinity Quaternary Pump User Manual 257 12 Hardware Information APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The sub
116. he pump is shipped with tubing and capillary connections installed between degassing unit MCGV pump heads pressure sensor and Multi Purpose Valve This section describes the installation of additional flow connections Parts required p n Description Other modules G4220 68755 Accessory Kit 5067 4644 Solvent Cabinet Kit 1290 Infinity Pump Preparations Pump is installed in the LC system 42 1290 Infinity Quaternary Pump User Manual Installing the Module 3 WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Remove the front cover by pressing the snap fasteners on both sides 2 Place the solvent cabinet on top of the UHPLC stack 3 Put the bottle head assemblies into empty solvent reservoirs and place the bottle in the solvent cabinet 4 Route tubing connections along the left side of the UHPLC stack using tube clips 5 Connect the inlet tubes of the bottle head assemblies to the inlet connectors A to D at
117. head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench This procedure describes how to assemble the secondary pump head using the pump head alignment tool Assembling the primary pump head can be done accordingly The secondary pump head has the heat exchanger capillary which must fit into the openings of the alignment tool whereas the primary pump head does not have a heat exchanger 1 Use the soft plastic side of the insert tool for inserting the A piston seal to the pump chamber housing Wet the piston aes Insert tool seal with isopropanol or another suitable solvent before insertion Pump seal o Se 1 i ol Pe f j Pump chamber housing 180 1290 Infinity Quaternary Pump User Manual Maintenance 9 Assembling the Pump Head 2 Insert the support ring and pump head ferrules into the piston housing Observe the pins on the support ring which help you assembling the pump head correctly Pins second pin not shown Pump head ferrules Support ring 3 Assemble the pump head by putting the pump chamber housing on top of the support ring Observe correct orientation of the pin Pump chamber housing Pump head ferrules Support ring Piston housing NOTE Do NOT install the inlet and outlet valves and the outlet filter at this stage 1290 Infinity Quaternary Pump User Manual 181
118. his product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex l this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1290 Infinity Quaternary Pump User Manual 299 14 Appendix Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 300 1290 Infinity Quaternary Pump User Manual Appendix 14 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991
119. imized data If your solvent is neither available in the user interface nor in the library please use generic solvents Generic aqueous gives good results for most solvent mixtures with at least 50 water which have similar properties as pure water For other solvents with high organic percentage Generic organic gives a good approximation Importing Solvent Calibration in ChemStation 1 Go to menu Instrument gt Instrument configuration 2 In the Instrument Configuration screen choose your module and click Configure Click Configure Solvent Type Catalogs In Solvent Type Catalogs click Import Navigate to the location of the solvent calibration table and click Open The new solvent will now appear in the Solvent Type Catalogs y Oo CFF F Q The imported solvent is now available for selection as a solvent type see Table 4 on page 60 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 88 Status indicators 89 Power Supply Indicator 89 Module Status Indicator 90 Available Tests vs User Interfaces 91 Agilent Lab Advisor Software 92 Overview about the troubleshooting and diagnostic features ee Agilent Technologies 87 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which in
120. inity Quaternary Pump Fixation screw inlet weaver to PIV not shown Outlet valve primary pump head Headless screw for 1290 Infinity pump heads Internal gold seal for Outlet Valve 221 10 Parts and Materials Secondary Pump Head Parts Secondary Pump Head With Seal Wash Quaternary Pump Figure 24 Secondary pump head Quaternary Pump with seal wash 222 1290 Infinity Quaternary Pump User Manual Item 10 11 12 13 14 15 17 p n 0515 1218 G4220 20003 G4220 81013 G4220 20028 G4220 20001 0905 1719 G4220 60016 5062 2484 0905 1718 64220 63010 5067 5678 64204 60004 1290 Infinity Quaternary Pump User Manual Parts and Materials 10 Description Screw M5 40 mm long Pump Head Screw Pump Head Front Plate order pump head Heat Exchanger secondary pump head only Headless screw for 1290 Infinity pump heads Spacer Fitting Pump Chamber Housing order pump head PE Seal Seal holder including backup ring Gasket seal wash pack of 6 Wash Seal PE Support Ring Seal Wash Pump Head Ferrules order pump head Piston Housing order pump head Piston 1290 Infinity Pumps ceramic Outlet filter 1290 Infinity Quaternary Pump 223 10 Parts and Materials 224 Secondary Pump Head Without Seal Wash Quaternary Pump Figure 25 Item 1 13 6 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 1 10 2 11 1 12 1 14 1 D gt N O DN 14 s Secondary pump head Quaternary Pump without seal w
121. iption Multi Purpose Valve Head Inline Filter Assembly OPTIONAL Frit 0 3 pm for inline filter 5 pk OPTIONAL Clamp for In Line Filter QPTIONAL Capillary ST 0 17 x 120 mm SLV SV 1290 Infinity Quaternary Pump Head Assembly with Seal Wash 1290 Infinity Quaternary Pump Head Assembly without Seal Wash 1290 Infinity Quaternary Pump Inlet Weaver Assembly Inlet tubing Multi channel gradient valve MCGV Blank plug for MCGV Degasser 4 Channels for Quaternary Pump Peristaltic pump with Pharmed tubing Pressure sensor 1200 bar Jet Weaver 380 uL for 1290 Infinity Quaternary Pump OPTIONAL 213 10 Parts and Materials Flow Connections Flow Connections 3 r 4 Va 2 al 1 p V CSE MDA oa DOS OCI i Y Oh 7 EY Figure 19 Flow connections of the pump Item p n Description 1 G4220 60007 Bottle Head Assembly 2 G4220 60035 Tubing kit 140 mm 2 pk degasser to MCGV 3 5067 4657 Capillary ST 0 17 mm x 300 mm pump to autosampler 4 5067 5416 Capillary ST 0 17 x 120 mm SLV SV for Jet Weaver 5 5067 4748 Capillary ST 0 17 mm x 90 mm Multi Purpose Valve to inline filter 6 5067 4656 Capillary ST 0 25 mm x 80 mm pressure sensor to outlet filter and Multi Purpose Valve 7 5067 4755 Flexible Waste Tube 5 m G4220 68070 Ultra Clean Tubing Kit includes bottle head assemblies and tubing connections within the pump G4220 60070 Tubing Kit 140 mm Ultra Clean Tubing tubes from
122. ision Flow accuracy Maximum operating pressure Pressure pulsation Compressibility compensation Gradient formation Delay volume Composition range Dual pistons in series pump with proprietary servo controlled variable stroke design power transmission by ball screws smooth motion control of pistons for active damping 0 001 5 mL min in 0 001 mL min increments lt 0 07 RSD or 0 01 min SD whatever is greater 0 2 5 0 mL min 1 or 10 pL min whatever is greater Operating range up to 120 MPa 1200 bar up to 2 mL min ramping down to 80 MPa 800 bar up to 5 mL min lt 1 amplitude or lt 0 5 MPa 5 bar whatever is greater Automatic pre defined based on mobile phase selection Low pressure quaternary mixing Standard configuration lt 350 pL With optional V380 Jet Weaver lt 500 uL Settable range 0 100 Executed in 300 pL st ep increments Based on retention time at constant room temperature Pumping degassed H20 at 10 MPa 100 bar At 1 mL min water Recommended range 1 99 or 5 pL min per channel whatever is greater 1290 Infinity Quaternary Pump User Manual Table 2 Site Requirements and Specifications 2 Performance specifications Type Specification Comments Composition precision Composition accuracy Integrated degassing unit Control Local control Communications Safety and maintenance GLP features Housing
123. ith a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Error ID 0070 Error Information 7 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition 1290 Infinity Quaternary Pump User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 97 7 98 Error Information Lost CAN Partner Error ID 0071 During an analysis the internal synchroni
124. itions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a pH range between 1 12 and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc 1290 Infinity Quaternary Pump User Manual 69 4 70 Using the Pump Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplie
125. l wash pump was missing when tried to turnon 119 seal wash installation 45 parts 215 secondary pump head disassemble 170 306 shutdown 96 site requirements 21 power cords 23 solvent counter exceeded limit 103 solvent handling 73 special interfaces 259 special settings boot resident 262 forced cold start 262 specification physical 25 specifications 21 performance 26 status indicator 90 system setup and installation optimizing stack configuration 31 system tool kit 231 T target pressure not reached for quaternary pump degasser 103 TCP IP parameter configuration 267 telnet configuration 288 temperature sensor 101 test functions 88 timeout wait for composition 115 timeout wait for flow 116 timeout wait for pressure 117 timeout wait for run volume 115 timeout wait for volume 116 timeout 96 transport foam remove 39 transport prepare 207 troubleshooting error messages status indicators 88 95 88 89 U unknown multi purpose valve type 112 unpacking 30 user interfaces 91 V valve hardware overcurrent MCGV 119 valve position filter flush mode 17 normal operating mode without mixer 14 purge mode 15 with Jet Weaver and inline filter 16 valve rail kit install 205 valve multi purpose 225 voltage range 25 W waste counter limit exceeded 104 weight 25 writing the multi purpose valve tag failed 112 writing the pump encoder tag failed 106 1290 Infinity Quaternary Pump User Manual
126. le 9 Maintenance This chapter describes the maintenance of the Agilent 1290 Infinity Quaternary Pump 10 Parts and Materials This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the pump in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the module to the controller software 14 Appendix This chapter provides additional information on safety legal and web 1290 Infinity Quaternary Pump User Manual Contents Contents 1 Introduction 9 Features 10 Overview of the Quaternary Pump 11 Operating Principle 12 Positions of the Multi Purpose Valve 14 System Overview 18 2 Site Requirements and Specifications 21 Site Requirements 22 Physical Specifications 25 Performance Specifications 26 3 Installing the Module 29 Unpacking the Module 30 Optimizing the Stack Configuration 31 Installation Information on Leak and Waste Handling 36 Removing the Transport Foam 39 Installing the Pump 40 Flow Connections tothe Pump 42 Installation of Seal Wash Function 45 4 Usingthe Pump 47 Leak and Waste Handling 48 Best Practices 49 Setting up the Pump with the Instrument Control Interface 53 Flushing the Filter 66 Solvent Information 68 Algae Growth in HPLC Systems 75 1290 Infini
127. le housing 3 Fix tubings of the peristaltic pump to the primary pump head outlet and secondary pump head inlet 1290 Infinity Quaternary Pump User Manual 147 9 Maintenance Replacing the Multi Channel Gradient Valve MCGV Tools required p n Description 0100 1710 Mounting Tool for Tubing Connections 8710 0899 Pozidriv screwdriver Parts required p n Description G1311 67701 Multi channel gradient valve MCGV Preparations Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages For best performance and life time use lower channels A and D for aqueous solvents in buffer applications see Operational Hints for the Multi Channel Gradient Valve MCGV on page 79 for details 1 Use the mounting tool for removing tubing connections Waste funnel between the degassing unit and the MCGV z Degassing unit MCGV 148 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Multi Channel Gradient Valve MCGV 2 Remove the inlet weaver see Replacing the Inlet 3 Remove the waste funnel Weaver on page 134 4 Remove the cover from the MCGV 5 Disconnect the MCGV cable 1 unscrew the two screws 2 3 and remove the valve 1290 Infinity Quaternary Pump User Manual 149 9 Maintenance Replacing the Multi Ch
128. lume the Indicates that an External Contacts board is installed area turns yellow when the bottle is empty the area turns red Clicking on the solvent bottle displays the Bottle Fillings dialog box The tooltip for the bottle shows the solvent name s R ia 7 bar The pressure setpoints The red line shows the current maximum pressure limit the green area shows the current pressure also shown as text The current solvent flow rate in mL min is displayed above the pressure display 1290 Infinity Quaternary Pump User Manual 55 4 56 Using the Pump Instrument Signals The following pump signals are displayed Flow The current solvent flow rate in mL min Pressure The current pump ressure in bar psi or MPa see Instrument Configuration on page 53 Tuning Indicates the tuning efforts of 1290 Infinity pumps For pumps operating as expected the signal should stay in a range of 1 to 1 within the full scale of 2 to 2 Pressure Limit The current maximum pressure limit Composition A B The contributions of channels A and B to the current solvent composition Composition C D The contributions of channels C and D to the current solvent composition Mixer The installed mixer type Valve position The current valve position 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Context Menu The context menu of the dashboard panel contains the following commands Control
129. lve allows easy software controlled switching between different modes of operation Normal Operating Mode Without Mixer In normal operating mode the flow comes from the pump head passes the pressure sensor and arrives at the central port of the Multi Purpose Valve The flow leaves the valve through port 4 to the system autosampler etc To system Figure 2 Valve position in normal operating mode without mixer 14 1290 Infinity Quaternary Pump User Manual Introduction Positions of the Multi Purpose Valve Purge Mode In purge mode the flow is diverted to the waste container From pump To waste Figure 3 Valve position in purge mode 1290 Infinity Quaternary Pump User Manual 1 15 1 Introduction Positions of the Multi Purpose Valve Normal Operating Mode With Jet Weaver and Optional Inline Filter In this mode the flow passes an optional Jet Weaver and the optional inline filter This configuration is recommended for special applications which require an increased mixing efficiency From pump Jet Weaver To system Inline filter Figure 4 Valve position in normal operating mode with Jet Weaver 16 1290 Infinity Quaternary Pump User Manual Introduction 1 Positions of the Multi Purpose Valve Filter Flush Mode This mode is used for cleaning the inline filter by back flushing it The flow goes to port 5 passes the inline filter in opposite direction and leaves to the waste through port 7
130. m pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common How to Prevent and or Reduce the Algae Problem e Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters e Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber 9301 1450 supplied with the instrument for your aqueous mobile phase e If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 1290 Infinity Quaternary Pump User Manual 75 4 Using the Pump Algae Growth in HPLC Systems 76 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 5 How to Optimize the Performance of Your Module Using the Degasser 78 Operational Hints for the Multi Channel Gradient Valve MCGV 79 Delay Volume and Extra Column Volume 80 Delay Volume 80 How to Configure the Optimum Delay Volume 81 How to Achieve Higher Resolution 83 Using Solvent Calibration Tables 86 This chapter gives hints on how to optimize the performance or use additional devices ot Agilent Technologies 77 5 How to Optimize the Performance of Your Module Using the Degasser The quaternary pump has a built in degasser which should alw
131. ments 21 install inline filter 198 valve rail kit 205 instrument layout 264 insufficient power of drive encoder LED 111 interfaces 253 internet 302 J jet weaver 1290 Infinity Quaternary Pump User Manual install 144 remove 141 L LAN automatic configuration with Bootp 277 Bootp amp Store 270 Bootp 269 cable 243 configuration switch 268 configuration 265 first steps 266 initialization mode selection 269 link configuration selection 276 manual configuration with telnet 288 manual configuration 287 PC and User Interface Software Setup 293 TCP IP parameter configuration 267 using default 271 using stored 271 eak sensor open 99 eak sensor short 98 eak 101 ine frequency 25 ine voltage 25 ink configuration selection 276 ost CAN partner 98 M MAC address determine 282 MAC address 266 maintenance feedback 263 introduction 125 replacing firmware 206 3 anual configuration of LAN 287 maximum stroke too short 114 MCGV replace 148 message remote timeout 97 3 ulti purpose valve failed 110 3 ulti purpose valve 225 positions 14 non operating altitude 25 non operating temperature 25 0 operating Altitude 25 operating principle 12 operating temperature 25 operational hints MCGV 79 optimization achieving higher resolution 83 stack configuration 31 outlet filter replace 196 outlet valve replace 138 overcurrent of pump drive 108 P packaging damaged 30 par
132. miniature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 258 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Table 8 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Re
133. minimizing the pressure ripple in order to achieve highest flow precision A stable flow can be delivered even in case of eventual small internal leaks which can be compensated automatically A heat exchanger between two pump chambers strongly reduces thermal effects due to solvent compression under very high pressures As solvents are compressed by the pump head and expand further down the flow path for example in the column the volumetric flow is changed depending on the compressibility of the liquid Agilent control software allows specifying pure solvents pre mixed solvents and solvent gradients Associated Agilent solvent libraries are used by the pump control for enhanced flow accuracy which is required for cross instrument or cross system reproducibility and method compatibility A high resolution encoder unit is attached to the pump drives which divides a single turn into 65000 steps Each step corresponds to a volume of about 300 pL which allows an extremely precise control 1290 Infinity Quaternary Pump User Manual Introduction 1 Operating Principle Inline filter optional Jet Weaver optional Multi Purpose Valve Pressure sensor Solvent bottles Degasser Multi channel gradient valve Inlet weaver Figure 1 The hydraulic path 1290 Infinity Quaternary Pump User Manual 13 1 Introduction Positions of the Multi Purpose Valve Positions of the Multi Purpose Valve The Multi Purpose Va
134. n G4204 60004 Outlet filter 1290 Infinity Quaternary Pump 1 Remove the capillary from the outlet filter to the pressure sensor 196 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Outlet Filter 2 Remove the outlet filter using a 14 mm wrench 3 Mount the new outlet filter Using a torque wrench set to 16 Nm is recommended 4 Mount the capillary connection to the pressure sensor 1290 Infinity Quaternary Pump User Manual 197 9 Maintenance Installing the Inline Filter For certain applications Agilent recommends using an optional inline filter which can be installed to the Multi Purpose Valve Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description G7104 68000 Inline Filter Upgrade Kit The kit includes 5067 5407 Inline Filter Assembly 5067 4748 Capillary ST 0 17 mm x 90 mm G4204 40000 Clamp for In Line Filter Preparations Turn the pump off 1 Remove the capillary between ports 5 and 8 from the 2 Clip the inline filter clamp to the Multi Purpose Valve Multi Purpose Valve 198 1290 Infinity Quaternary Pump User Manual Maintenance 9 Installing the Inline Filter 3 Connect the 90 mm capillary part of the upgrade kit to 4 Fix the inline filter to the clamp the filter outlet 5 Install the integrated capillary of the inline filter to port5 6 In
135. n case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface 1290 Infinity Quaternary Pump User Manual 95 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 96 1290 Infinity Quaternary Pump User Manual Probable cause 1 Leak detected in another module w
136. n is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing 1290 Infinity Quaternary Pump User Manual 71 4 72 Using the Pump fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide ZrO Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated r
137. nary Pump User Manual 243 11 Identifying Cables RS 232 Cable Kit p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 244 1290 Infinity Quaternary Pump User Manual Identifying Cables 11 Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 1290 Infinity Quaternary Pump User Manual 245 11 Identifying Cables Agilent 1200 Module to Printer 246 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 12 Hardware Information Firmware Description 248 Electrical Connections 251 Rear View of the Module 252 Interfaces 253 Overview Interfaces 256 Setting the 8 bit Configuration Switch 260 Special Settings 262 Early Maintenance Feedback 263 Instrument Layout 264 This chapter describes the pump in more detail on hardware and electronics ot Agilent Technologies 247 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections a non instrumen
138. nstalling the Pump 40 Flow Connections tothe Pump 42 Installation of Seal Wash Function 45 This chapter gives information about the preferred stack setup for your system and the installation of your Agilent 1290 Infinity Quaternary Pump Apg Agilent Technologies 29 3 Installing the Module Unpacking the Module Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 30 Ensure all parts and materials have been delivered with your module The delivery checklist is included to your shipment For parts identification please check the illustrated parts breakdown in Parts and Materials on page 211 Please report any missing or damaged parts to your local Agilent Technologies sales and service office 1290 Infinity Quaternary Pump User Manual Installing the Module 3 Optimizing the Stack Configuration If your module i
139. nstant Pilot G4208A The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 13 on page 269 1290 Infinity Quaternary Pump User Manual 267 13 LAN Configuration Configuration Switch The configuration switch can be accessed at the rear of the module Z hose ho Ganesan LLULLU tinie oeeocnno Tt hg een ET ee Figure 37 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above To perform any LAN configuration SW1 and SW2 must be set to OFF Table 12 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Initialization Mode Selection on page 269 Link Configuration speed and duplex mode determined by auto negotiation for details see Link Configuration Selection on page 276 268 1290 Infinity Quaternary Pump User Manual LAN Configuration 13 Initialization Mode Selection Table 13 The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON
140. nuts If the optional inline filter is not installed connect ports 5 and 8 with a capillary Capillary ST 0 17 x 120 mm SLV SV 5067 5416 1290 Infinity Quaternary Pump User Manual 193 9 Maintenance Replacing Parts of the Multi Purpose Valve Tools required p n Description 8710 2394 9 64 inch hex key Parts required p n Description 1534 4045 Bearing ring 5068 0123 Rotor seal Multi Purpose Valve 1290 Infinity Quaternary Pump 1200 bar 5068 0120 Stator ring 5068 0001 Stator head 1535 4857 Stator screws 10 Pk Preparations Remove all capillary connections from the Multi Purpose Valve 1 Use the 9 64 inch hex key for opening the valve head 2 Replace parts as required 194 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing Parts of the Multi Purpose Valve 3 Reassemble the valve head and mount it to the valve drive Bearing ring Rotor seal Stator ring Stator head Stator screws 1290 Infinity Quaternary Pump User Manual 195 9 Maintenance Replacing the Outlet Filter When For removing blockages and leaks in the high pressure filter assembly The outlet filter should be replaced as required depending on the system usage Other parts are covered by the Agilent Preventive Maintenance PM Service Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Torque wrench Torque wrench head 14 mm for torque wrench Parts required p n Descriptio
141. olecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and that can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be offset by reducing the temperature of the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve shows that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns In isocratic separations increasing the retention factor k results in better resolution because the solute is retained longer In gradient separations the retention is described by k in the following equation pefo F 100 A B V S m 1290 Infinity Quaternary Pump User Manual How to Optimize the Performance of Your Module 5 where k mean k value tg time length of gradient or segment of gradient min F flow ml min Vm column delay volume e A B change in fraction of solven
142. olvent cabinet Leak pan Leak pan s outlet port A leak funnel B and corrugated waste tube C Waste tube of the sampler s needle wash Condense drain outlet of the autosampler cooler Waste tube of the purge valve Waste tube NY oo Fi wo DM 1290 Infinity Quaternary Pump User Manual 37 3 Installing the Module 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 11 on page 37 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect capillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual WARNING Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off gt Avoid loops gt Tubes must not sag gt Do not bend tubes gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module Warning Attention Warnung Attenzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant
143. ong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 9 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the 4 screws at the rear of the alignment tool Tighten screws crosswise ZX This procedure will align pump head parts to their correct positions and close the pump head tightly 184 1290 Infinity Quaternary Pump User Manual Maintenance 9 10 Open the 3 screws which have closed the pump head alignment tool and take out the aligned pump head In case the pump head sticks inside the alignment tool you can use the handle and insert it to the rear of the tool for pushing out the pump head Heat exchanger capillary 00 an T 9 NI CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 11 For the primary pump head install the inlet valve and outlet valve using the torque wrench which is included to the 1290 Infinity Service Kit p n 5067 4699 Set 10 Nm for the inlet valve and 10 Nm for the outlet valve 1290 Infinity Quaternary Pump User Manual 185 9 Maintenance Assembling the Pump Head 12 Push in a thin pin for removing the old filter frit from the filter assembly
144. ot correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1290 Infinity Quaternary Pump User Manual In This Guide In This Guide This manual covers the Agilent 1290 Infinity Quaternary Pump G4204A 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Module This chapter gives information about the preferred stack setup for your system and the installation of your Agilent 1290 Infinity Quaternary Pump 4 Using the Pump This chapter explains the operational parameters of the Agilent 1290 Infinity Quaternary Pump 5 How to Optimize the Performance of Your Module This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 1290 Infinity Quaternary Pump User Manual 3 In This Guide 8 Test Functions and Calibrations This chapter will describe the tests for the modu
145. p User Manual Maintenance 9 Replacing the Multi Purpose Valve Tools required p n Description 5023 0240 Hex driver 14 slitted Parts required p n Description 0100 1259 Blank nut plastic 01080 83202 Blank nut stainless steel 5067 4174 Multi Purpose Valve Head Preparations Remove all capillary connections from the Multi Purpose Valve 1 Remove the clamp with the inline filter Inlet filter n ly 1290 Infinity Quaternary Pump User Manual 191 9 Maintenance Replacing the Multi Purpose Valve 2 Unscrew the black union nut and remove the head of the 3 Put the new valve head onto the valve drive such that the purge valve by pulling it to the front lobe fits to the groove Screw the valve head onto the valve drive using the union nut 2 D s d WA DN 192 1290 Infinity Quaternary Pump User Manual Maintenance 9 The central C port is connected to the outlet of the pressure sensor Port 1 is Port 2 is Port 3 is Port 4 is Port 5 is Port 6 is Port 7 is Port 8 is connected to connected to blocked by a connected to connected to blocked by a connected to connected to the outlet of the optional Jet Weaver the inlet of the optional Jet Weaver blank nut plastic the system typically autosampler the outlet of the optional inline filter blank nut SST the waste capillary the inlet of the optional inline filter Block unused ports with blank
146. pensation Sensor Open 99 Compensation Sensor Short 100 Fan Failed 101 Leak 101 Pump Error Messages 102 Pressure of quaternary pump above upper limit 102 Pressure below lower limit 102 Target pressure not reached for quaternary pump degasser 103 Solvent counter exceeded limit 103 Waste counter limit exceeded 104 Flow rate limit exceeded 104 Quaternary pump shutdown during analysis 105 Reading the pump encoder tag failed 105 Writing the pump encoder tag failed 106 Pump drive blocked or encoder failed 106 Drive current too low 107 Drive current too high 107 Drive timeout 108 Overcurrent of pump drive 108 Deliver underrun 109 Be Agilent Technologies 93 7 Error Information Defect connection between main board and pump drive encoder 109 Pump drive encoder defect 109 Multi Purpose Valve failed 110 Reading of multi purpose valve tag failed 110 Pump drive encoder rollover 111 Drive position limit 111 Insufficient power of drive encoder LED 111 Drive encoder error 112 Writing the multi purpose valve tag failed 112 Unknown multi purpose valve type 112 Pump drive encoder error 113 Pump drive error 113 Maximum stroke is too short 114 Pump drive stop notfound 114 Timeout Wait for Composition 115 Timeout Wait forrun volume 115 Timeout Wait for Volume 116 Timeout Wait for Flow 116 Timeout Waidt for Pressure 117 Drive Encoder failed 117 Drive phases differ too much in electric resistance 118 Degasser s pressure limit
147. r is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative Please contact your Agilent service representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being pinched by a metal component Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Loose connection between the power switch board and the main board 2 Defective power switch board 3 Defective main board 1290 Infinity Quaternary Pump User Manual Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 99 7 Error Information Compensa
148. r of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides 1290 Infinity Quaternary Pump User Manual Usingthe Pump 4 Stainless Steel ST Stainless st
149. rdware Information Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 10 Boot Resident Settings On board LAN Mode Select swi sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 1 0 0 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 11 Forced Cold Start Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 0 0 0 1
150. rrent LAN settings ip lt X X X xX gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return 1290 Infinity Quaternary Pump User Manual 289 13 LAN Configuration 5 Use the and press Enter to list the current settings SACI EE eee information about the LAN interface 7 MAC address initialization mode LAN Status Page Initialization mode is Using Stored active TCP IP settings TCP IP status here ready Init Mode Using Stored connected to PC with controller software e g Agilent ChemStation here not connected MAC Addre 6636D36AG838 TCP IP Properties active IP Address gt 134 40 27 95 Subnet Mask gt 255 255 248 0 Gateway 134 40 24 1 Controllers no connections Figure 53 Telnet Current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list current settings WINDOWS system32 cmd_exe telnet 134 40 27 95 STEEL CMLAT change of IP setting to gt 4 Initialization mode is Using Stored LAN Status Page active TCP IP settin
151. ry Pump User Manual Maintenance 9 Removing the Jet Weaver Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 0899 Pozidriv screwdriver Parts required p n Description 2 0100 1259 Plastic fittings Select Do not use mixer in ChemStation Switch off the pump at the main power switch Preparations 1 Remove capillary connections from the Jet Weaver to the Multi Purpose Valve 1290 Infinity Quaternary Pump User Manual 141 9 Maintenance Removing the Jet Weaver 2 Open the screw which fixes the Jet Weaver to the front 3 Lift up the Jet Weaver 1 and pull it out of the front panel panel 142 1290 Infinity Quaternary Pump User Manual Maintenance 9 Removing the Jet Weaver 4 If no other Jet Weaver shall be installed use plastic fittings for closing unused ports of the valve and install the metal lid Otherwise continue at Installing the Jet Weaver on page 144 1290 Infinity Quaternary Pump User Manual 143 9 Maintenance Installing the Jet Weaver When The optional Jet Weaver 380 uL for 1290 Infinity Quaternary Pump G4204 68000 is available for applications which require highest mixing performance see chapter Optimizing Performance Tools required Description Screwdriver Pozidriv 1 Parts required p n Description 1 G4204 68000 Jet Weaver 380 uL for 1290 Infinity Quaternary Pump containing 2 5067 5416 Capillary ST 0 17
152. s because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 22 1290 Infinity Quaternary Pump User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The
153. s firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whi d 69761 250 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1290 Infinity Quaternary Pump User Manual 251 12 Hardware Information Electrical Connections Rear View of the Module
154. s maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1290 Infinity Quaternary Pump User Manual 263 12 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emis
155. s part of a complete Agilent 1290 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume For other possible configurations please refer to the Agilent 1290 Infinity System Manual One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Quaternary LC System in the following configuration see Figure 7 on page 32 and Figure 8 on page 33 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required The Agilent 1290 Infinity Quaternary Pump should always be installed at the bottom of the stack 1290 Infinity Quaternary Pump User Manual 31 3 Installing the Module Optimizing the Stack Configuration Instant Pilot Solvent cabinet Detector Column compartment Autosampler Figure 7 Recommended stack configuration for 1290 Infinity with quaternary pump front view 32 1290 Infinity Quaternary Pump User Manual Installing the Module Optimizing the Stack Configuration LAN to control software NUMA UU HN UA CAN Bus cable to Instant Pilot Analog detector signal optional AC Power CAN Bus cable Figure 8 Recommended stack configuration for 1290 Infinity with quaternary pump rear vi
156. sions from the instrument itself 264 1290 Infinity Quaternary Pump User Manual 1290 Infinity Quaternary Pump User Manual 13 LAN Configuration What You Have To Do First 266 TCP IP Parameter Configuration 267 Configuration Switch 268 Initialization Mode Selection 269 Dynamic Host Configuration Protocol DHCP 273 General Information DHCP 273 Setup DHCP 274 Link Configuration Selection 276 Automatic configuration with Bootp 277 About Agilent BootP Service 277 How BootP Service Works 278 Situation Cannot Establish LAN Communication 278 Installation of BootP Service 279 Two Methods to Determine the MAC Address 281 Assigning IP Addresses Using the Agilent BootP Service 282 Changing the IP Address of an Instrument Using the Agilent BootP Service 285 Manual Configuration 287 With Telnet 288 With the Instant Pilot G4208A 292 PC and User Interface Software Setup Setup 293 PC Setup for Local Configuration 293 User Interface Software Setup 294 This chapter provides information on connecting the module to the controller software oh Agilent Technologies 265 13 LAN Configuration What You Have To Do First The module has an on board LAN communication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the
157. stall the removable capillary of the inline filter to port 8 of the Multi Purpose Valve of the Multi Purpose Valve 1290 Infinity Quaternary Pump User Manual 199 9 Maintenance Removing the Inline Filter Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description 5067 5416 Capillary ST 0 17 x 120 mm SLV SV 1 Remove the capillaries from the Multi Purpose Valve to the inline filter 200 1290 Infinity Quaternary Pump User Manual Maintenance 9 Removing the Inline Filter 2 Remove the clamp with the inline filter 3 Install the capillary between ports 5 and 8 of the Multi Purpose Valve 1290 Infinity Quaternary Pump User Manual 201 9 Maintenance Replacing Parts of the Inline Filter Tools required p n 8710 0510 Parts required p n 5067 5407 5067 4748 5023 0271 5067 5416 Description Wrench open 1 4 5 16 inch Description Inline Filter Assembly Capillary ST 0 17 mm x 90 mm Multi Purpose Valve to inline filter Frit 0 3 pm for inline filter 5 pk Capillary ST 0 17 x 120 mm SLV SV CAUTION Stuck Capillary in Multi Purpose Valve Shortcutting the inline filter by directly connecting its right capillary to valve port 5 can damage the Multi Purpose Valve The size position of this capillary in its fitting is incompatible so it may get stuck irreversibly to the valve gt Do not shortcut the filter by directl
158. t 1200 Infinity including all plastic parts Name plate 1260 Front panel solvent cabinet Leak panel Solvent bottle amber Solvent bottle transparent Bottle Head Assembly 227 10 Parts and Materials Cover Parts Cover Parts Figure 29 Item p n 1 5067 5396 2 5042 9964 3 5067 4683 4 5042 8914 228 Cover parts Description 1290 Infinity Quaternary Pump Cover Kit base top left right Name plate for Agilent 1290 series Front Panel Serial number plate 1290 Infinity Quaternary Pump User Manual Leak Parts Parts and Materials 10 Leak Parts iM Figure 30 Leak parts Item p n 1 5041 8389 5041 8388 5062 2463 G1361 47100 5042 9922 G4280 40016 5041 8381 xn oO oO AeA WwW N 1290 Infinity Quaternary Pump User Manual Description Leak funnel holder Leak funnel Corrugated tubing PP 6 5 mm id 5m Sealing lip Leak panel Power Switch Coupler ZL Power switch button 229 10 Parts and Materials Accessory Kit 230 The Accessory Kit 1290 Infinity Quaternary Pump G4204 68705 contains ltem 1 1 1 3 1 4 1 5 1 6 1 7 1 8 1 9 1 10 4 11 1 p n 0100 1816 5067 4755 5063 6527 5181 1519 5042 9967 5067 4657 5067 4670 5067 5443 9301 6476 5042 9972 5023 0271 Description Fitting Waste Tube to Purge Valve Flexible Waste Tube 5 m Leak tubing assembly 1 m CAN cable Agilent module to module 1 m Tubing clip set of 5 clips Capillary ST 0 17 mm x
159. t B during the gradient e S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constant and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can generate higher peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008 Maximizing peak capacity and separation speed in liquid chromatography 1290 Infinity Quaternary Pump User Manual 85 5 How to Optimize the Performance of Your Module Using Solvent Calibration Tables 86 Importing Solvent Calibration Tables RC NET based Agilent graphical user interfaces ChemStation EZChrom Elite OpenLab etc include data for most commonly used solvents in HPLC This data contains solvent properties and is used for optimum pump control in order to ensure best flow and composition accuracy If your solvent is not included to the software please check the Agilent web site http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 for additional libraries registration required which also provides updates and opt
160. t has been set has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly for example the flow rate Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes Timeout Wait for Flow Error ID 29183 A target condition flow rate has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands manual changes 116 1290 Infinity Quaternary Pump User Manual Error Information 7 Timeout Waidt for Pressure Error ID 29185 A target condition pressure has been sent to the instrument which should have been reached within an expected time frame but didn t Either the limit time frame or the current value of the variable has been modified later directly or indirectly Probable cause Suggested actions 1 Incorrect parameters have been senttothe Verify control software macros manual instrument by the control software or commands m
161. t specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote e error handling diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 248 1290 Infinity Quaternary Pump User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using the following tools latest version should be used Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files on a USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PP
162. ternary Pump User Manual Maintenance 9 Releasing a Stuck Inlet Valve 2 Open the fitting at the center of the multi channel gradient valve MCGV Do not open the screw marked with the red cross Remove the inlet weaver from the MCGV 3 Slightly open the black plastic screw at the bottom of the inlet valve and rotate the inlet weaver to the front Then retighten the screw 4 Disconnect the capillary from the pressure sensor inlet and route the rapan to a small beaker Capillary Beaker 5 Fill the syringe with a suitable wash solvent NOTE For salt deposits warm water is a good choice For organic deposits use ethanol or acetone 1290 Infinity Quaternary Pump User Manual 153 9 Maintenance Releasing a Stuck Inlet Valve 6 Connect the syringe and adapter to the inlet weaver 7 Push the syringe for flushing the inlet valve and pump head 8 Restore original connections Flush the system for several minutes 154 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Pump Head When Tools required Parts required Preparations CAUTION For preventive maintenance or in case of problems with the pump performance p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20015 Adapter in square to hex p n Description G4204 60200 1290 Infinity Quaternary Pump Head Assembly with Seal Wash G4204 60400
163. th additives For example you can blend 100 water with 10 isopropanol in water Channel The channel name Type The type of solvent Solvent lt n gt Pure solvent Solvent lt n gt Additive Solvent mixture Calibration Click the down arrow and select the solvent or solvent mixture from the list Name Enter a name for the solvent or solvent mixture in this field Stock conc For solvent mixtures specify the concentration of the additive in the stock solution in this field Pure solvents are always 100 Final conc Enter the concentration of the additive that you want to achieve in this field The pure solvent and solvent mixture will be blended to achieve the Final conc For the relationship of stock concentration and concentration in the mixture the composition accuracy needs to be considered see Performance Specifications on page 26 Conc unit The concentration can be defined as mM mmol L or as 1290 Infinity Quaternary Pump User Manual 65 4 Using the Pump Flushing the Filter For highest performance and robustness the 1290 Infinity Quaternary Pump uses 3 solvent filters 1 Solvent inlet filter 20 um pore size 5041 2168 as part of Bottle Head Assembly G4220 60007 have a large pore size of about 20 um and filter out particles before they reach the pump Solvent inlet filter ce 2 An outlet filter average pore size 5 um Outlet filter 1290 Infinity Quaternary Pump G4204 600
164. th of this module Part Materials Degasser chamber TFE PDD copolymer PFA internal tubings PEEK inlets FEP tubings ETFE fittings Ultra clean tubings PFA tubings PEEK fittings Microfluidic structures2 SST MCGV PEEK FEP PFA Al 03 based ceramic ruby sapphire SST Passive inlet valve SST gold ruby ZrO5 based ceramic tantalum Outlet valve SST gold ruby ZrOj based ceramic tantalum Pump head SST Pistons Zr04 based ceramic Piston wash seals UHMW PE SST Pressure sensor SST Multi Purpose Valve Polyimide SST DLC 1 Ultra clean tubings are available for the use with high end MS detectors They are also compatible to THF 2 Inlet Weaver Jet Weaver Heat Exchanger 68 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable cond
165. the left hand side of the vacuum degasser Fix the tubes in the tubing grommets of the pump Tubing clips Degasser inlets Tubing grommets 1290 Infinity Quaternary Pump User Manual 43 3 Installing the Module Flow Connections to the Pump 6 Connect the capillary from the autosampler to port 4 of the Multi Purpose Valve 7 Connect the waste tubing to port 7 of the Multi Purpose Valve and place it in your waste system To waste system To autosampler From solvent bottles 8 Ifthe pump is not part of an Agilent 1290 Infinity system stack or placed on the bottom of a stack connect the waste tube to the waste outlet of the pump leak handling system 9 Fill solvent reservoirs with your mobile phase 10 Fill solvent lines with a syringe prime purge and condition your pump before first use 44 1290 Infinity Quaternary Pump User Manual Installing the Module 3 Installation of Seal Wash Function Installation of Seal Wash Function To wash solvent reservoir To waste container The 1290 Infinity Quaternary Pump has a built in seal wash function The Seal Wash Function is recommended when using buffers or other non volatile solvents or additives that could deposit on pistons and seals It is used for regularly cleaning these parts automatically 1 Place a wash solvent reservoir into the solvent cabinet A mixture of distilled
166. this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1290 Infinity Quaternary Pump User Manual Appendix 14 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 s T
167. tion Sensor Short Error ID 0080 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective power switch board Please contact your Agilent service representative 2 Loose connection between the power Please contact your Agilent service switch board and the main board representative 3 Defective main board Please contact your Agilent service representative 100 1290 Infinity Quaternary Pump User Manual Error Information 7 Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Pleas
168. tions 1 Pump drive encoder is defect Please contact your Agilent service representative Pump drive error Error ID 29212 29213 The pump drive failed during calibration Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual 113 7 114 Error Information Maximum stroke is too short Error ID 29203 The maximum stroke is too short During initialization the pump defines the operation position of the pump drives and therefore the pistons First the pump drive moves backwards to find a mechanical stop within the ball screw Afterwards pistons move forwards for finding the maximum available stroke volume These values are expected within a pre defined range Maximum stroke too short means that the outer drive position is too close This can be caused by a drive initialization without pump head or if the pump head has not been installed properly screws are loose e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Wiper shifted Please contact your Agilent service representative 2 Pump head blocks piston movement Replace clean or repair pump head 3 Pump drive motor is mechanically blocked Please contact your Agilent service representative Pump drive stop not found Error ID 29207 The pump drive stop has not been found e Par
169. tomated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting 1290 Infinity Quaternary Pump User Manual 83 5 84 How to Optimize the Performance of Your Module The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give a higher plate number The pressure rises with the inverse square of the particle size and proportionally with the length of the column This is the reason that the 1290 Infinity LC system was designed to go to 1200 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small m
170. tout d placement Entfernen Sie das Netzkabel bevor Sie das Ger t bewegen gees Scollegare il cavo di alimentazione prima di spostare lo strumento Desconectar el aparato de la red antes de moverlo Figure 12 Warning label illustration for correct waste tubing 38 1290 Infinity Quaternary Pump User Manual Installing the Module 3 Removing the Transport Foam Removing the Transport Foam 1 Open the front cover of the module AN 2 Carfully remove the protective foam 3 Close the front cover 1290 Infinity Quaternary Pump User Manual 39 3 Installing the Module Installing the Pump Installing the Pump Parts required Description 1 Pump 1 Power cord 1 Agilent Control Software and or Instant Pilot G4208 Preparations Locate bench space Provide power connections Unpack the pump 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the module is OFF switch stands out Status indicator Sh Jolien Techasingisc t29 inishy M Power switch W s T Serial number Figure 13 Front view of the quaternary pump 40 1290 Infinity Quaternary Pump User Manual Installing the Module 3 Installing the Pump 3 Connect the power cable to the power connector at the back of the module 4 Connect the required interface cables to the rear of the pump I APG Remote Ei z Ae SSS Sc
171. ts accessory kit 230 cover 228 damaged 30 missing 30 multi purpose valve 225 overview 212 1290 Infinity Quaternary Pump User Manual Index pump head assembly 216 pump service kit 232 sealwash 215 system tool kit 231 PC and User Interface Software Setup 293 performance specifications 26 performance Optimization 77 physical specifications 25 power consideration 22 power consumption 25 power cords 23 power supply indicator 89 power switch 40 pressure below lower limit 102 pressure exceeded upper pressure limit 102 pressure sensor replace 131 131 primary pump head disassemble 165 pump drive blocked or encoder failed 106 ump drive encoder defect 109 ump drive encoder error 113 ump drive encoder rollover 111 p p p pump drive error 113 pump drive stop not found 114 pump error messages 102 pump head assembly parts 216 pump head assemble 179 disassemble 163 replace 155 pump features 10 overview 11 305 Index Q quaternary pump shutdown during analysis 105 reading of multi purpose valve tag failed 110 reading the pump encoder tag failed 105 remote cable 238 remove inline filter 200 repairs replacing firmware 206 replace inline filter 202 outlet filter 196 replacing Multi Purpose Valve parts 194 Multi Purpose Valve 191 pump head 155 resolution Optimization 83 RS 232C cable 244 S safety class 297 safety general information 297 standards 25 symbols 296 sea
172. ty Quaternary Pump User Manual Contents 5 How to Optimize the Performance of Your Module 77 Using the Degasser 78 Operational Hints for the Multi Channel Gradient Valve MCGV 79 Delay Volume and Extra Column Volume 80 How to Configure the Optimum Delay Volume 81 How to Achieve Higher Resolution 83 Using Solvent Calibration Tables 86 6 Troubleshooting and Diagnostics 87 Overview of the Module s Indicators and Test Functions 88 Status indicators 89 Available Tests vs User Interfaces 91 Agilent Lab Advisor Software 92 7 Error Information 93 What Are Error Messages 95 General Error Messages 96 Pump Error Messages 102 8 Test Functions and Calibrations 121 9 Maintenance 123 Introduction to Maintenance 125 Warnings and Cautions 126 Overview of Maintenance 128 Cleaning the Module 129 Installing Fittings and Capillaries 130 Replacing the Pressure Sensor 131 Replacing the Inlet Weaver 134 Replacing the Inlet Valve 136 Replacing the Outlet Valve 138 Removing the Jet Weaver 141 Installing the Jet Weaver 144 Replacing the Seal Wash Pump 146 Replacing the Multi Channel Gradient Valve MCGV 148 Releasing a Stuck Inlet Valve 152 Replacing the Pump Head 155 6 1290 Infinity Quaternary Pump User Manual Contents Disassembling the Pump Head 163 Disassembling the Primary Pump Head 165 Disassembling the Secondary Pump Head 170 Replacing the Heat Exchanger 174 Replacing Wash Seal and Gasket 177 Assembling the Pump Head 1
173. uaternary Pump User Manual 89 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 90 When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold st
174. ubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al Os are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications 1290 Infinity Quaternary Pump User Manual Using the Pump 4 Solvent Handling Handling of Buffers The following recommendations should be observed when using buffer solutions Buffers and aqueous solutions are possible sources of algae contamination for avoiding related problems please read Algae Growth in HPLC Systems on page 75 For buffer concentrations of 0 1 M or higher using the seal wash function periodically with a runtime of 0 3 min every 3 min is strongly recommended Filter buffer solutions to avoid increased wear or blockages that are caused by undissolved
175. vice main board representative 2 Defect tag Defect connection between tag Please contact your Agilent service and encoder representative Pump drive blocked or encoder failed Error ID 29214 Pump drive blocked or encoder failed Parameter None Probable cause Suggested actions 1 Blockage of the pump drive Drive encoder Please contact your Agilent service failed representative 106 1290 Infinity Quaternary Pump User Manual Error Information 7 Drive current too low Error ID 29205 The current consumption of the pump drive is too low e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Drive motor defect Please contact your Agilent service representative 2 Wrong missing connection of pump drive to Please contact your Agilent service main board representative Drive current too high Error ID 29236 The current consumption of the pump drive is too high e Parameter 1 2 referring to pump drive Probable cause Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit Multi Purpose Valve heat exchanger 2 Drive motor defect Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual 107 7 Error Information Drive timeout Error ID 29204 Movement of drive during initialization is blocked mechanically e Parameter 1 2 referring to pump drive Probable cause Suggest
176. wer screws is marked on the module cover First tighten these screws and then tighten the upper screws Screws 1290 Infinity Quaternary Pump User Manual 205 9 Maintenance Replacing Module Firmware When Tools required OR OR Parts required Preparations 206 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module 1290 Infinity Quaternary Pump User Manual M
177. x 120 mm SLV SV Jet Weaver to Multi Purpose Valve Preparations Switch off the pump at the main power switch 1 Open the screw of the Jet Weaver metal lid 144 1290 Infinity Quaternary Pump User Manual Maintenance 9 Installing the Jet Weaver 2 Remove the metal lid by lifting it up 1 and pulling it out of the front panel 2 4 Mount both capillary connections to the Jet Weaver observing the correct orientation 3 Insert the Jet Weaver to the opening in the front panel 1 and push it down 2 SS lt 5 Connect the inlet capillary of the Jet Weaver to port 2 of the Multi Purpose Valve Connect the outlet capillary to port 1 6 Configure the Jet Weaver as mixer in the user interface see Instrument Configuration on page 53 1290 Infinity Quaternary Pump User Manual 145 9 Maintenance arinn tha Caal Wach D acl ine o al Wasn rump Replacing the Seal Wash Pump When In case of wear of the seal wash pump Parts required p n Description 5065 4445 Peristaltic pump with Pharmed tubing 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5m Preparations Remove the flow connections from and to the seal wash pump 1 For removing the seal wash pump press the clips 1 and pull the pump to the front 2 146 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing the Seal Wash Pump 2 Insert the pump clips to the holes in the modu
178. y connecting its right capillary to valve port 5 in case the inline filter cannot or shall not be used gt Use Capillary ST 0 17 x 120 mm SLV SV 5067 5416 instead The inline filter can be cleaned using the back flush function in the user interface of your Agilent instrument control software 202 1290 Infinity Quaternary Pump User Manual Maintenance 9 Replacing Parts of the Inline Filter 1 Remove the capillaries from the Multi Purpose Valve to the inline filter 2 Remove the inline filter from the clamp attached to the 3 Use two 5 16 wrenches for opening the inline filter Inline filter 1290 Infinity Quaternary Pump User Manual 203 9 Maintenance Replacing Parts of the Inline Filter 4 Replace the filter frit and reassemble the inline filter Filter frit 5 Put the inline filter to the clamp and install its capillaries The integrated capillary is connected to port 5 of the Multi Purpose Valve The removable capillary is connected to port 8 Removable capillary Integrated capillary 204 1290 Infinity Quaternary Pump User Manual Maintenance 9 Installing the Valve Rail Kit When This rail is needed for the installation of external valves Tools required Description Pozidrive screwdriver 1 Parts required p n Description 1 5067 4634 Valve Rail Kit 1 The valve rail is fixed to the pump cover by 4 screws The position of the lo
179. zation or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause 1 CAN cable disconnected 2 Defective CAN cable 3 Defective main board in another module Leak Sensor Short Error ID 0082 Suggested actions Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly Exchange the CAN cable Switch off the system Restart the system and determine which module or modules are not recognized by the system The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective leak sensor 2 Leak sensor incorrectly routed being pinched by a metal component Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative 1290 Infinity Quaternary Pump User Manual Error Information 7 Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak senso

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