Heraeus Biofuge Haemo - User manual
Contents
1. 17 Operation Opening the lid Should it be necessary to open the lid manually carry Press the lid key The lid lock mechanism is released OUt the following steps and the lid pops open 1 Make sure the rotor stands still consult window in Emergency lid release the lid In case of a power failure you cannot open the lid nor 2 Unplug the mains plug mally using the lid key see previous section TO 3 Push a thin screwdriver or another suitable tool permit unloading even in this case the centrifuge is horizontally from each equipped with a mechanical lid unlocking system How T side through the two ever you may use this system only in case of emer A openings in the side genoy NN P panels of the centri PN fuge see figure Rotor can spin at high speed Touch s Push the locking pins ing it may cause severe injuries E under the side panels Z Always wait for several minutes until p p d Eoi y E E ped n the rotor has come to a complete stop Without power the brake does not function and braking takes much longer than normal unlocks audibly Re move the auxiliary tools and open the lid 4 If the rotor still turns close lid immediately and wait until it has come to a complete stop Never brake the rotor using your hands or tools 5 As soon as the rotor stands still remove your samples and close the lid 18 Loading the hematocrit rotor After filling and sealing th2. tion of the warranty period Maintenance and care Warranty conditions The warranty period starts with the day of delivery Within the warranty period the centrifuge is repaired or replaced free of cost if there are provable faults in ma terials or workmanship Conditions for a warranty are e the centrifuge is used according to the instructions of use e mounting extensions settings alterations or repairs are carried out exclusively by personnel authorized by KENDRO e the required maintenance and care procedures are carried out regularly 43 Troubleshooting Troubleshooting Problems you can handle yourself Ee If problems other than those described in the following tables arise you must consult your nearest au thorized service Error Behavior of the centrifuge Possible cause s and measures to be taken Displays remain dark The motor stops The rotor stops without braking The lid cannot be opened Mains failure or not connected 1 Check the mains connection 2 If the mains connection is OK call the nearest Service Displays fail briefly The motor stops suddenly The rotor stops without braking The display reads br see br Brief interruption of mains supply 1 Check whether the plug is plugged in properly 2 Restart the centrifuge Lid cannot be opened Pressing the lid key has no effect Lid not correctly engaged or lid warped Press lid down in t
3. with quick start run time passed Keys lid open lid only possible if connected with mains supply quick run short term acceleration as long as key is pressed with indication of run time passed start stop normal start or manual stop set speed time selection of the parameter to be changed speed or run time stepwise increase decrease of preset values for the selected parameter speed or run time accelerated change when pressed permanently Error codes troubleshooting see chapter Troubleshooting E 1 E 12 keys do not respond main board defective E 3 no braking current lid popped open or manually opened during run excess tem perature in the drive br power turned off during run or power failure lid lid opened during run OPEN lid lock mechanism out of order Contents Contents For your safety oett 3 Before use oce eee release 15 Proper use gud 3 Where to install the centrifuge 15 Improper use 4 ette eee e eec 3 Mains connection 15 Centrifuging hazardous materials 3 Removing the transport protection 15 Handling osen cid eee edic plns 4 Operation eseri Pm 17 Conformity to current standards 5 Transport and installation 2 17 Safety instructions in this manual 5 Mains coli CHO A ecce aoreet ata Salts 17 The Biofuge haemo 7 Opening the Ti
4. 3759 Other accessories e Reading harp 7600 0938 e Reading spiral 7500 1236 For use with the fixed angle rotor also available e fixed angle rotor 24 x 1 5 2 ml 7500 3325 e special cap nut for fixing the rotor cap nut tubular socket order no wrench e 10 mm tubular hexagon wrench for tightening the 70056208 order no cap nut 2036 0072 The printed documents consist of the delivery notes and this Manual 13 Accessories for your notes 14 Before use Where to install the centrifuge The centrifuge must be operated in a place meeting the following criteria e A safety zone of 30 cm around the centrifuge must be maintained Hazardous materials must not be kept within this zone during centrifugation e The substructure must be stable and resonance free A good support is provided by a plane labora tory bench or a large laboratory carriage with cast ers that may be locked e To ensure sufficient air circulation a minimum dis tance from the wall of 10 cm at the back and of 15 cm on each side must be kept e The centrifuge must be protected from heat and direct sunshine e The location should be well ventilated Before use Mains connection Make sure that your mains voltage and frequency match the specifications on the instrument Removing the transport protection Turn the instrument on The display panel shows for about 6 s the routine internal software check sequence Open the lid by pressing the open lid key and
5. C to 40 C Pre cooling in the freezer is not permitted Lifetime of the rotor There are no restrictions to the service life of the high performance rotor 7500 3325 B However please ob serve the following due to safety reasons Rotors and accessories made of plas tic should not be exposed to direct sunlight and UV rays If the rotor shows signs of discolor ation deformation or wear or is out of balance it must be exchanged straight away 23 Operation Removing the rotor To remove the rotor you must follow the steps de scribed above in reverse order With the hermetic lid you may in case of contamination separate the rotor from the drive without opening the lid In this case you can open rotor upon removal from the centrifuge using e g a safety work bench before decontaminating it 1 Open the lid of the centrifuge 2 Remove rotor cap not necessary with the hermetic lid 3 Screw the cap nut open by turning it counterclock wise using the socket wrench delivered with the in strument Remove the cap nut 4 Grab the rotor in the middle and pull gently upwards off the drive shaft Be careful not to jam it Danger of irreparable motor damage Never tilt the rotor Always grab it in the middle and pull out perpendicu larly 24 Loading the rotor As an accessory you can optionally order the fixed angle rotor 7500 3325 with 24 places Three sets of adapters and a
6. by eye How ever you must nonetheless ensure that opposite tubes are filled to the same level 25 Operation Aerosol tight application The following steps have to be carried out e Lubricate the seals before inserting them lubricant order no 75003500 e Insert the seal C profile in the groove at the side of the body of the rotor e Insert the O ring into the inner groove on the screw on top only with screw on top 75003326 and not with open container lids 26 Attention Please check that your sample containers are suitable for the centrifugal application desired 16060 x g temperature in uncooled devices approx 10 K above room temperature Please observe the permissible filling volumes Nominal volume Permissible volume 2 0 ml 1 5 ml 1 5 ml 1 0 ml others 21 nominal volume The sealing elements are to be checked regularly for damage to the shape and surface Exchange faulty parts immediately spare sealing rings 75003268 The snap on lid is not suitable for aerosol tight application A Checking for aerosol tightness A Check the aerosol tightness of your rotor whenever appropriate To carry out the test proceed as follows Carefully clean and degrease the rotor chamber wall then attach an adhesive white paper strip about 4 x 2 cm so that liquid leaking out of the ro tor may precipitate on it Fill all places of the respective rotor w
7. come into contact with the drive shaft and the ball bearing Organic solvents may decompose the lubricant of the motor bearing The drive shaft may block Maintenance and care Disinfection If a centrifuge tube containing infectious material leaks during a run you have to disinfect the centrifuge im mediately A Infectious material could enter the centri fuge if spills or tube breakage occur Danger of infection may occur upon contact Take appropriate protective meas ures for personnel Mind the permissible filling volumes and loading limits for the tubes In case of contamination the operator has to make sure that no further persons are jeopardized Contaminated parts have to be decontami nated immediately If required further protective measures have to be initiated 39 Maintenance and care Rotor and rotor chamber must be treated with a neu tral universal disinfectant Best suited for this purpose are disinfectant sprays ensuring that all rotor and accessory surfaces are covered evenly e Please use 70 ethanol for disinfection handling hints when applying these A Please note the safety measures and substances For other disinfectants please consult KENDRO Services 40 w N gt You may disinfect the rotor and the accessories as described in the following section Be sure to follow the pertinent safety procedures for handling infec tious material Pu
8. frictional heat This leads to a temperature increase of the rotor the tubes and finally of the samples The extent of warming depends on e runtime e temperature of the environment e location of the centrifuge e rotor speed The Biofuge haemo is equipped with air cooling to prevent overheating of the samples The cooling is particularly effective in the cold room lowest permissi ble temperature 4 C Quick run operation If you press and hold the quick run key the rotor is accelerated with maximum force up to the maximum speed Accessories Standard accessories Hematocrit dish and reading harp The Biofuge haemo is delivered with a special rotor hematocrit dish or rotor equipped with 24 guide not ches for receiving blood capillaries A reading harp for determining hematocrit values after removing the capillaries is delivered with the instru ment Reading spiral As an option you can order a reading spiral which is placed directly onto the rotor for reading hematocrit values in situ Accessories Accessories Hematocrit rotor Table 1 Hematocrit rotor order no 7500 1067 for biofuge haemo number of samples 24 blood capillaries temperature range 4 C to 40 C maximum capacity 24 capillaries with 1 2 to 1 8 mm diameter and 75 mm length maximum speed Nmax 12000 maximum RCF value at Nmax 14926 10 Additional accessories Fixed angle rotor 7
9. remove the transport protection for the rotor Check that the rotor moves freely by lightly turning it and make sure the rotor is tightly screwed on 15 Before use for your notes 16 Operation Transport and installation Damage to the centrifuge by jolting Transport the centrifuge only in the upright position using the special box provided with the instrument and secure it properly Place the centri fuge carefully Make sure the rotor transport protection has been removed before starting the instrument CS The Biofuge haemo is now ready for use Mains connection Make sure that your mains voltage and frequency match the specifications on the instrument before con necting the centrifuge to the mains supply The instrument does not have a mains switch Operation After you have established the mains connection the following reading appears in the control panel This tells you that the instrument carries out an internal check of its soft Biofuge Heraeus ware If the lid is closed the display in the panels speed and time changes to 0 after a couple of seconds With opened lid the speed panel displays ae m the message OPEN instead Biofuge If you do not intervene the display changes to the standby mode after 2 minutes The displays turn dark however the centrifuge is ready for use at any time This is signaled by the flashing red light in the time panel
10. run time Preselected run time ssssesssss Continuous operation Starting the centrifuge sssssssss Changing the settings during the run Displaying the preset values Stopping the centrifuge Stopping with preset time Stopping with continuous operation Short time centrifugation RCF value Measuring the hematocrit value Reading harp poreca ER Reading spiral sse General hints concerning hematocrit measurements ssssse ee Maintenance and care Maintenance to be performed by the customer a re a e PE recte ENE SEES E SKR Disinfection Decontamination ccccceeeeseceeeeeeeeeeeenees Autoclaving The KENDRO service offer Warranty conditions Troubleshooting Problems you can handle yourself In case you must call the Service Technical data Component parts and performance Electrical connections fuses Speed g diagram for rotor 7500 3325 Autoclaving protocol For your safety Heraeus centrifuges are manufactured according to current technical standards and regulations Nonethe less centrifuges may pose dangers if e they are not used as designed e they are operated by untrained personnel e their design is improperly changed e the safety instructions are not heeded Therefore anybody concerned w
11. 2 Unscrew and remove the rotor cap 3 Loosen the rotor fastening nut counterclockwise using a coin Grab the rotor centrally at the hub and pull out perpendicularly Do not tilt The following description applies only to the fixed angle rotor 7500 3325 If you use the centrifuge only for he matocrit measurements you may skip this section and continue on page 29 Selecting the speed Important application information for rotor 7500 3325 To attach the rotor use the acorn nut with ball head Order no 70056208 To tighten the acorn nut use the 10 mm tubular hexa gon box spanner Order no 20360072 Please always close your microlitre containers care fully Open container lids can damage the rotor lid For some special applications the container lids must remain unsealed If this is the case please use the screw top order no 75003326 instead of the standard snap on lid order no 70901111 Operation Position the snap on lid Press the rotor lid down onto the rotor until the snap on catch engages onto the ball of the acorn nut same Remove the snap on lid By activating the grip cap the lock is re leased and the rotor lid can be removed 21 Operation Inserting the rotor Possible damage to drive and rotor 30 C You may insert the rotor only if the temperature of the drive the rotor and the cap nut is between 10 C and Possible damage to drive and rotor Do not push d
12. 500 3325 for microliter tubes Optionally you can order for the Biofuge haemo a fixed angle rotor with 24 holes for placing microliter tubes with a volume of 1 5 or 2 0 ml In addition three sets of adapters with 24 reduction sleeves each are available on request With the adapt ers you can centrifuge all commercially available mi croliter tubes with volumes of 0 2 ml to 0 6 ml as well as 0 2 ml PCR reaction vessels Please refer to our sales documents for detailed infor mation on accessories including technical data and order numbers Accessories 11 Accessories Fixed angle rotor technical data Table 1 Fixed angle rotor order no 7500 3325 for Biofuge haemo no of places volume 24x 1 5 2 ml maximum permissible load 24x4g temperature range 4 C to 40 C maximum speed Nmax min 13000 maximum RCF value at Nmax 16060 minimum speed Nmin min 2000 minimum RCF value at nmin 380 maximum radius cm 8 5 minimum radius cm 5 9 angle of incidence 40 maximum kinetic energy s 1 65 kNm 12 Adapters for rotor order no 7500 3325 Table 2 Adapters for the fixed angle rotor of the Biofuge haemo Accessories Adapter Dimensions Capacity Number per Color Order No x H Set reduction sleeve PCR 6 2 x 20 mm 0 2 ml 24 gray 7600 3750 reduction sleeve 8x43 5 mm 0 5 0 6 ml 24 turquoise 7600 3758 reduction sleeve 6 x 46 mm 0 25 0 4 ml 24 red 7600
13. Heraeus Biofuge haemo Instructions for use A P pn N B eh UNO N e p d zn ET cut g s How to use this manual Use this manual to get acquainted with your centri fuge and its accessories The manual helps you to avoid inappropriate han dling Make sure to keep it always close to the centri fuge A manual that is not kept handy cannot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessories Transportation and hook up Use of the centrifuge Maintenance and care Troubleshooting Technical data Index Overleaf you will find a graphic repre sentation of the control panel of the Biofuge haemo with a survey of the most important functions Please fold out speed set Biofuge haemo start E stop El Heraeus The control panel of the Biofuge haemo Display Speed Resting state During run Braking End Lid open Resting end upon pressing any key display of preselected speed after 2 min switch to standby mode flashing red light preselected speed or o OPEN error codes if present Run time time Resting end During run upon pressing any key display of preselected run time after 2 min switch to standby mode flashing red light remaining run time or
14. IEC 1010 2 EN 61010 2 020 ae General hints are marked with this symbol Pollution degree 2 Overvoltage category II In addition you are asked to adhere to the pertinent regulations in Germany for 120 V only e Regulations for prevention of accidents VBG 4 e CAN CSA C22 2 No 1010 1 92 e Regulations for prevention of accidents VBG 5 e CAN CSA C22 2 No 1010 2 020 94 e Regulations for prevention of accidents VBG 7z For your safety for your notes The Biofuge haemo The Biofuge haemo The figure below shows the Biofuge haemo with the lid opened In this state the standard display at the end of a run and after opening the lid is OPEN After a cou ple of minutes the display changes to the standby mode and all display panels turn dark aan END a a Biofuge Heraeus Safety systems The Biofuge haemo is equipped with a number of safety systems Rotor chamber The rotor chamber is integrated into the steel case When the lid is closed the rotor chamber is sealed against the surroundings by a rubber ring with a spe cial profile that allows cooling air to pass through the rotor chamber Warning if lid is manually opened during a run or if lid lock mechanism is out of order If the lid is manually opened during a run or if the lid lock mechanism is out of order a corresponding mes sage appears in the display Lid and OPEN respectively Lid lock mechanism You can open the lid only when t
15. aintenance and care Disinfection Strongly corrosive substances that may cause dam age to materials and impair the mechanical strength of the rotor may be centrifuged only inside protec tive vessels Handling Never use the centrifuge unless the rotor is properly mounted Never manually open the lid if the rotor still turns Use only original parts for the centrifuge The only exception are common glass or plastic centrifuge tubes if these are approved for the rotor speed and RCF values of your rotor respectively Never use the centrifuge with the lid open Never use the centrifuge if the paneling has been partially or totally removed Changes in mechanical or electrical components may be carried out only by persons authorized to this effect by KENDRO Laboratory Products You may use the centrifuge only with a properly loaded rotor You must not overload the rotor If the rotor or the lid shows visible traces of corro sion or wear you must stop using it Strictly follow the rules and regulations for cleaning and disinfection For your safety Conformity to current standards Safety instructions in this manual Heraeus centrifuges are manufactured and tested This symbol denotes potential hazards to according to the following standards and regulations A persons C AX This symbol denotes potential damage to the centrifuge or parts in its immediate surround for all voltages Ings e IEC 1010 1 EN 61010 1 e
16. an standard DIN 58933 describes a practical standard The procedure outlined in this manual has been designed in accordance with this standard We recommend all users to obtain a copy of it Operation for your notes 37 Maintenance and care Maintenance and care Maintenance to be performed by the cus tomer For the protection of persons environment and mate rial you are obliged to clean the centrifuge regularly and to disinfect it if necessary Unsuitable cleaning agents or disinfec tion procedures may damage the cen trifuge and its accessories If you intend to use cleaning agents or disinfection procedures not rec ommended by the manufacturer you have to make sure by consulting the manufacturer that the procedure foreseen does not cause any dam ages to the instrument 38 Cleaning oe Clean the casing the rotor chamber the rotor and the accessories regularly and in case of need This is indi cated both for reasons of hygiene and to prevent corro sion due to contamination sticking to the instrument and its accessories Clean them with mild agents of pH values ranging from 6 to 8 For other cleaning agents please consult KENDRO Services Pull mains plug before cleaning the instrument Immediately after cleaning dry the aluminum parts or put them into a warm air dryer at a temperature not exceeding 50 C During cleaning liquids and espe cially organic solvents should not
17. crit values you must remove the capillaries one by one from the hema tocrit dish Place the capillary with the lower end of the blood col umn on the zero line and the upper end on the 100 line of the reading harp At the boundary between the erythrocytes and the plasma you can now read the percentage of packed cells Be careful to place the the capillary at right angles to the zero line Reading spiral The reading spiral is available as additional accessory To measure hematocrit values remove the rotor cap see page 20 and place the spiral on the hematocrit dish which may remain in the centrifuge Using the outer knurled eccentric screw turn the zero line so that it comes to lie on the outer end of the blood col umn then place the 100 line of the reading spiral over the meniscus using the knurled screw in the mid dle 36 You can now read the hematocrit value directly from above on the graduation Once you are finished you can dispose of all capillaries at once Loosen the rotor fastening screw with a coin by turning it counterclock wise lift the hematocrit dish vertically out of the centri fuge and eject the capillaries into the waste disposal by overturning the dish General hints concerning hematocrit measurements A certain amount of standardization is necessary to obtain reproducible results both in the interest of the patients and blood donors and for the sake of compa rability of sciencific data The Germ
18. d ore dit udis ir cin ho haad 18 Safety systems editi diede 7 Emergency lid release 18 Rotor chamber ede eden ase 7 Loading the hematocrit rotor 19 Lid lock mechanism erecta etos 7 Inserting the hematocrit rotor 19 Properties ar naen a a a tee ae 8 Important application information for Quick run operation eeeeseseeneee 8 rotor 7900 3329 1 5 e cnet 21 Accessories oe cccccccccccocccncccccccccecceuceeccenceecess 9 Inserting the rotor 0 0 eee eee eee eee teeeeeeeetees 22 Standard accessories sss 9 Permissible rotor temperature 23 Hematocrit dish and reading harp 9 Lifetime of the rotor 23 Reading spiral an ce ipae heated te 9 Removing the rotor ssseeseee 24 Hematocrit rotor s 10 Loading the rotor 1 scenerne 24 Fixed angle rotor 7500 3325 for microliter tubes11 Maximum loading een 25 Fixed angle rotor technical data 12 Filling the tubes 25 Adapters for rotor order no 7500 3325 13 Aerosobtight application ns 26 Other accessories sesssnntes 13 Please observe the permissible filling volumes 26 Checking for aerosol tightness 27 Placing the tubes in the rotor 28 Contents Selecting the speed sssssessees Selecting the
19. e capillaries place them into the guide notches with the sealed ends pointing out wards The centrifuge must always be loaded weight symmetrically to avoid unbal ance unsteady runs and subsequent damage Inserting the hematocrit rotor After checking for correct loading you may insert the rotor into the centrifuge Possible damage to drive and rotor Do not push down the rotor by force If the rotor is tilted carefully remove and reinsert it To put the rotor into position proceed as follows 1 Open the lid and make sure that the rotor chamber is clean Remove eventual dust foreign material or sample residues Operation Turn the rotor so that the driving pin of the motor shaft fits into the groove on the underside of the ro tor and slide the rotor onto the drive shaft until the rotor hub rests on the shaft cf Figure 19 Operation 3 Tighten the fastening nut clockwise using a coin 4 Screw the rotor cap on with the plastic knob You must fasten the rotor cap before starting the centrifuge Starting and stopping the centrifuge is described start ing on page 31 evaluation of the haematocrit on page 36 20 Removing the hematocrit rotor For removing the hematocrit rotor you must carry out the steps described above under Inserting the hema tocrit rotor in reverse order Irreparable damage to the motor Never tilt the rotor during removal 1 Open the centrifuge lid
20. e of tube breakage 38 installation 55 place of 15 K key start 31 keys general operation 8 L lid blockage 43 lid lock built in safety system 7 lid open during run warning 7 lid release emergency 7 18 manual 18 mains connection 15 mains switch not present 17 56 maintenance 37 manual lid release 18 steps for 18 maximum sample density 3 maximum sample volume 8 O operation continuous 30 33 preselected run time 30 short time 33 organic solvents not allowed for cleaning 38 original parts mandatory use 4 overloading dangers implied 25 P partial loading of rotor 28 pathogenic microorganisms protection against 4 permissible volume calculation of for dense samples 25 preset values display 32 problems handling of 43 protective vessels for corrosive substances 4 Q quick run 8 not for hematocrit measurements 33 quick run function 33 quick run key 8 R radius of centrifugation for calculation of RCF value 34 RCF value 10 34 reading harp 13 for hematocrit 35 standard accessory 9 reading spiral 13 for hematocrit 35 optional accessory 9 readings of control panel during run 8 relative centrifugal force 34 rotor approved 22 insertion 22 loading 28 partial loading 28 removal 24 rotor cap 23 24 mandatory fastening 20 rotor chamber technical data 47 rotor insertion tools for 22 run time fine tuning 30 for hematocrit measurements 30 range 30 setting 30 running n
21. entrifuge Possible cause s and measures to be taken Lid appears in the display Motor stops Rotor stops without braking Lid lock mechanism out of order 1 Press the lid shut The centrifuge stops without braking 2 If error persists call nearest Service Display OPEN appears although lid is closed Start impossible Control lid lock mechanism The lock must be engaged on both sides If error persists call nearest Service Flashing red point in time panel Displays remain dark Not an error instrument is on standby Press any key With start stop or quick run the centrifuge starts running at once With the lid key the lid pops open and the display reads OPEN In case you must call the Service Should you require our Service please tell us the order no and serial number of the instrument You find the pertinent information at the back of the instrument near the socket for the mains plug 46 Troubleshooting 47 Technical data Technical data Component parts and performance Part function Description Design Armored case steel Frame torsion resistant hot galvanized 4 mm steel plates Body and front panel impact resistant highly dampening plastic Lid stove enameled steel plate Keys and display panel Keys and display panel covered with easy care protective foil Material impact resistant plastic Dimensions diameter x height 222 mm x 110 mm Electro
22. he middle of the front section Heat monitoring relays in the lid unlocking magnets have been actuated repeated pressing of the lid key within a few seconds Press key again after a short pause approx 1 min 44 Troubleshooting Error Behavior of the centrifuge Possible cause s and measures to be taken Centrifuge is exceptionally 1 Stop the centrifuge by pressing the stop key in case of noisy emergency pull mains plug 2 Wait until the centrifuge stands still 3 Check whether the rotor is properly loaded 4 Check whether a broken vessel damage to the rotor or motor malfunction was responsible for the noise 5 If you cannot locate and solve the problem call Service br Mains connection interrupted If the plug was pulled inadvertently plug in again during run Wait for about 75 seconds The centrifuge comes to a stop without braking E 1 or E 12 Rotor does not start Pull mains plug and plug in again If the error persists call Service E 3 No braking current drive out Lid opened during run of order rotor stops without braking Wait until the rotor has come to a complete stop check window in the lid Pull mains plug and plug in again If error persists call nearest Service Motor overheated Room temperature too high or insufficient air cooling Pull mains plug and wait 30 min Check all vents and clean with small brush 45 Troubleshooting Error Behavior of the c
23. he power is turned on and the rotor has practically come to a halt lt 80 rpm You can start the centrifuge only if the lid is properly closed Emergency lid release In order to permit you to remove samples even after a power failure the centrifuge is equipped with an emer gency lid release see Operation The Biofuge haemo Properties The Biofuge haemo is an air cooled benchtop centri fuge for the determination of the hematocrit It can also be used for the preparation of sensitive samples in the biochemical and medical laboratory The rotor accepts common centrifuge tubes up to a volume of 2 ml The preset speed is reached in seconds You can also spin samples for only a few seconds using the quick run key if this is required for the task in question The long lived maintenance free induction motor provides quiet and vibration free operation even at high speeds The user friendly control panel permits easy operation At the start the preset speed and run time are dis played During a run the remaining run time is dis played above 1 min in minutes and below 1 min in seconds If you press the or key repeatedly you increase the corresponding preset parameter speed or run time stepwise If you press and hold down the chosen key the respective value changes continuously at first slowly and after a few seconds at an accelerated pace Air cooling of the Biofuge haemo During a run the spinning rotor creates
24. hermetic lid are available for this rotor see under Accessories Improper or improperly combined ac cessories may cause severe damage to the centrifuge Use only the parts and accessories listed in the chapter Accessories Maximum loading Overloading may cause the rotor to explode Exploding parts may severely damage the centrifuge Never exceed the maximum permissi ble load of 4 g per place The Biofuge haemo can reach high rotational speeds implying enormous centrifugal force The rotor is de signed in a way warranting sufficient residual strength even at the highest permissible speed However this safety system presupposes that the maximum permissible load of the rotor is not exceeded The maximum load for the rotor has been calculated for a sample density of 1 2 g cm If the density of your sample is higher you must either reduce its volume to an overall weight of 4 g or calculate a reduced speed Nperm according to the following formula weight of tube with sample 1 2 g cm 3 n n Perm MaX weight of tube with denser sample Operation Filling the tubes Separations by centrifugation function best when the unbalance of the rotor is minimized because separated zones are not perturbed by vibration It is therefore important to balance the centrifuge tubes as well as possible To minimize unbalance you should fill the tubes as evenly as possible You can achieve this
25. ith operation and maintenance of the centrifuge must read and follow the safety instructions In addition the pertinent regulations for prevention of accidents must be strictly followed Ez This manual is an integral part of the centri fuge assembly and must be kept close at hand at all times For your safety Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively The maximum sample den sity is 1 2 g cm at maximum speed Improper use During a run a safety zone of 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be stationed The centrifuge may cause harm to you or other per sons and may damage material goods if you do not respect the following safety measures Centrifuging hazardous materials e The centrifuge is neither made inert nor is it explo sion proof Therefore never use the centrifuge in an explosion prone environment e Explosive or flammable substances must not be centrifuged The same holds for substances prone to react briskly with each other For your safety Do not centrifuge toxic or radioactive substances or pathogenic microorganisms unless you have taken proper precautions Such precautions can e g consist of biological seals Should toxins or pathogenic substances enter the centrifuge or its parts you must carry out the proper procedures for disinfection see M
26. ith water according to the following Table Insert the rotor into the centrifuge and fasten it Carefully place the amount of test liquid 0 5 sodium fluorescein in water specified in the col umn leakage test into the lower part of the rotor within a virtual circle comprising the vessel bores not the bores themselves using a pipette or sy ringe Operation Place the rotor lid on top and screw it on ATTENTION Make sure that there is no spilled test liquid on the rotor clean if necessary Carry out a test run for 10 minutes at maximum rotor speed and 23 C ambient temperature Check the paper strip under UV light preferentially in a darkened room If there is no detectable fluorescence the test is considered passed Finally rinse rotor rotor lid and lid seal in running water and allow to dry 27 Operation Placing the tubes in the rotor The rotor must be loaded symmetrically When loading the rotor only partially you must ensure that opposite bores always receive tubes of equal weight when cen trifuging a single sample place a centrifuge tube e g filled with water The following figure gives examples for proper loading properly loaded rotors 28 Improper loading can in the worst case lead to damage to rotor and centrifuge Unbalance not only causes a noisy run but rapidly damages the motor suspension improperly loaded rotors After loading the rotor snap on the rotor cap unti
27. l it locks into place The optional hermetic lid 75003326 is screwed onto the rotor with the central nut Close the lid of the centrifuge by firmly pressing it down There must be a clicking sound and the lid must be locked so that it cannot be opened manually Selecting the speed For hematocrit measurements the speed is limited to maximally 12 000 rpm The minimum speed of the drive is 1 600 rpm the maximum speed 13 000 rpm The built in microproces sor prevents higher or lower speed settings Between these extremes you can select the speed in steps of 100 rpm using the following procedure 1 Press the set key in the speed panel press twice if the centrifuge is in the standby mode The speed last entered appears in the display The third digit from the right in the example the figure 5 flashes thus indicating that you can change the setting in multiples of 100 rpm Press one of the set keys for an increase or for a decrease By pressing the selected key briefly you increase or decrease the speed in steps of 100 rpm This option is supposed to be used for small changes and fine tuning If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace Operation m Un mme Lr jm 5 Ca jo a lo lo speed time 4 Release the key as soon as you are close to the desired value and fine tune if necessary by repeat edly pres
28. lay keeps flashing The run time is stored by again pressing one of the set keys or start Continuous operation For continuous operation press the set key in the time panel and then the key until hd for hold appears in the display With this setting the centrifuge keeps running until stopped manually Please note that the lifetime of centrifuge tubes par ticularly plastic ones is limited and that they may be damaged by continuous operation Operation Starting the centrifuge After startup alle LEDs except the red one for stop start the centrifuge This is signalled by the lit green LEDs to the right of the keys quick run and start stop see Figure If you are not sure which values you have prese lected you can determine them by pressing one of the set keys The function not currently selected displays 0 irrespective of the actual setting Press the start stop key in the control panel The dis play panel shows the preselected values for speed and run time see Figure below The centrifuge accelerates to the preselected value Simultaneously the run time display starts going backward from the preset time giving the remaining run time in minutes After reaching the last minute the display switches to seconds remain ing If you have chosen continuous operation the time dis play goes forward instead of backward 31 Operation speed time Biofuge Changi
29. ll mains plug Unscrew the rotor chuck Grab the rotor with both hands and pull it perpen dicularly off the drive shaft Remove the centrifuge tubes and adapters and disinfect them or dispose of them as necessary Treat the rotor and the rotor lid according to the instructions given for the disinfectant soaking in liq uid or spraying You must strictly observe the specified action times Turn the rotor head down and drain off the disinfec tant Thereafter thoroughly rinse rotor and lid with water Dispose of the disinfectant according to valid regula tions Aluminum rotors have to be treated with anticorro sive protective oil subsequently Disinfection with bleaching lye These agents contain highly aggres sive hypochlorites and must not be used with aluminum rotors To protect the rotor 75003325 as far as possible you must take the following precautions 1 Avoid high temperatures The bleaching solution and the rotor should not be warmer than ca 25 C 2 Do not let the bleaching solution act longer than absolutely necessary 3 After disinfection rinse the rotor thoroughly with distilled water and allow to dry Maintenance and care Decontamination For general radioactive decontamination use a solution of equal parts of 70 ethanol 10 SDS and water Follow this with ethanol rinses then de ionized water rinses and dry with a soft absorbent cloth cs Dispose
30. magnetic release with lid key when connected to mains 48 Part function Start Stop Short term start and stop respectively Indication of operating state End of centrifugation parameter memory parameter display Speed selection Run time selection Time display in quick run mode diagnostics Technical data Description start stop key start stop key Quick run key short term run when pressed permanently stop when released Preset values for speed and run time ar displayed during the run The panels speed and time read 0 e speed e runtime adjustable in steps of 100 min in the range of 1600 min to 13000 min adjustable in minutes from 1 min to 99 min hd mode continuous operation between 1 s and 60 s in seconds over 1 min in minutes e lid not properly closed display OPEN e indication of general malfunctions ERROR codes 49 Technical data Parameter Value description environmental conditions indoor use max elevation 2000 m above sea level max relative humidity 80 up to 31 C linearly decreasing down to 50 relative humidity at 40 C admissible temperature of the environ ment 4 C to 35 C during operation 10 C to 50 C during storage and transport dimensions 240 mm x 284 mm x 375 mm H x W x D weight without rotor 14 5 kg maximum speed 13 000 rpm hematocrit rotor 12 000 rpm ma
31. ng the settings during the run You can increase the speed setting for the current run by pressing set in the speed panel adjusting the value with the key and again pressing set If you use the same procedure to lower the speed by press ing instead of the centrifuge brakes to standstill and then accelerates again to the newly selected speed Likewise you can alter the preset run time during a run by pressing consecutively set in the time panel or and again set 32 Displaying the preset values You can check the preset values for speed and run time by pressing one of the set keys If you press the key again the display turns dark speed time Biofuge Heraeus Stopping the centrifuge Stopping with preset time Normally the run time has been preselected and all you have to do is wait until the centrifuge terminates the run automatically During braking the speed panel reads br and the time panel 0 Operation speed time E Biofuge Heraeus INSTRUMENTS Shortly before the rotor stops a two step acoustic sig nal alerts you to the fact As soon as the rotor stands still both panels display the message 0 and the LED next to the lid key lights up By pressing the lid key you can now open the lid and remove your samples The speed panel displays OPEN After 2 minutes the instrument switches to the standby mode and all displays turn da
32. of all washing solutions in appropri ate radioactive waste containers 41 Maintenance and care Autoclaving a Check whether autoclaving is permitted You may autoclave the rotor and the adapters at 121 C Maximum permissible autoclaving cycle 20 min at 121 C CE For reasons of safety you may autoclave the rotor 7500 3325 maximally 10 times The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on an even surface to avoid deformation Chemical additives to the steam are not permitted 42 Never exceed the maximum permis sible values for autoclaving tempera ture and autoclaving time Should the rotor show signs of wear you must stop using it The KENDRO service offer Kendro Laboratory Products recommends annual ser vicing of the centrifuge and the accessories by author ized customer service or trained professionals The customer service personnel is inspecting the electrical installations the suitability of the location the lid lock mechanism and the safety circuit the rotor e the rotor fastening and the drive shaft Defective material is exchanged KENDRO offers inspection and service contracts covering it Inspection costs are charged as flat rate contracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after expira
33. of diverse instruments since it is independent of the instrument used The only values entered in the equation are radius and speed of centrifugation 2 RCF 1118 1000 r radius of centrifugation in cm n speed in rpm The centrifuge reaches an RCF value of 16 060 at the maximum speed of 13 000 min Check carefully whether your tubes are designed for such a strain and reduce the speed if necessary Operation The maximum RCF value refers to the maximum radius of the tube For the fixed angle rotor 7500 3325 the above formula yields for the bottom of the bore radius 8 5 cm a maximum RCF value of 16 060 13 000 RZB 11 18 FS 8 5 16 060 1000 Please note that this value becomes lower depending on the tubes and adapters used You may take this into account when calculating the RCF value for your application The figure on the last page of this manual gives a graphic representation of the relation between speed and RCF Apart from the maximum RCF value RCF max lower line this graph also shows the minimum RCF value RCF min calculated for the meniscus of the sample up per line 35 Operation Measuring the hematocrit value The hematocrit value is determined after removal of the rotor cap see page 20 There are two evaluation me thods reading harp and reading spiral Reading harp The reading harp is the standard accessory delivered with the centrifuge To read the hemato
34. oise 44 RZB Wert 12 S safety instructions 3 4 safety measures 3 safety standards 5 safety systems built in 7 safety zone 3 57 30 cm around centrifuge 15 sample maximum density 25 volume reduction for dense samples 25 sample density maximum 3 sample volume maximum 8 service 42 service contracts 42 setting run time 30 speed 29 settings change during run 31 short time operation 33 site of installation 15 sodium fluorescein test solution for aerosol tightness 27 software check internal 17 speed fine tuning 29 range 29 setting 29 speed of centrifugation for calculation of RCF value 34 standardization of hematocrit measurements 35 standby mode 7 58 start key 31 starting run 31 stopping 32 substructure 15 symbols for hazards and dangers 5 T temperature regulation 8 tools for inserting rotor 22 toxins protection against 4 transport precautions for 17 transport protection removal 15 tube breakage with infectious material 38 tubes for centrifugation 8 types 11 volume range 11 V volume calculation of permissible v for dense samples 25 volume of sample maximum 8 W warning lid open during run 7 warranty conditions 42 59 For Ordering or Technical Information Asia Pacific Kendro Laboratory Products H K Limited Hong Kong Tel 852 2711 3910 Fax 852 2711 3858 info cn kendro spx com Europe Middle East Africa Kendro Laboratory Products International Sale
35. own the rotor by force If you cannot screw on the cap nut eas ily remove the rotor and reinsert it The rotor approved for the Biofuge haemo is shown in the chapter Accessories Check whether the rotor you want to insert corresponds to the figure in this chapter To insert the rotor you need the rotor the cap nut and the tubular socket wrench supplied see chapter Ac cessories pieces delivered 22 To put the rotor into position proceed as follows 1 Open the lid and make sure that the rotor chamber is clean Remove eventual dust foreign material or sample residues The thread and the O ring on the motor shaft must be in perfect condition Turn the rotor so that the notch for engaging the drive shaft points downward Place the rotor on top of the drive shaft so that the notch of the rotor is located precisely above the re taining pin Push the rotor gently down until the thread is com pletely laid bare see figure 5 If you have placed the rotor correctly you can screw on the cap nut easily and secure it with the tubular socket wrench delivered with the instrument 6 Snap the rotor cap onto the rotor the optional her metic lid 7500 3326 is screwed on centrally with the lid nut Ee Regularly check the proper positioning of the rotor and re tighten the cap nut as needed Operation Permissible rotor temperature The rotor 7500 3325 may be used only within a temperature range of 4
36. rk You can even now open the lid by pressing the lid key You can stop the centrifuge at any time by pressing the stop key 33 Operation Stopping with continuous operation If you have chosen continuous operation you must stop the centrifuge manually by pressing the stop key The centrifuge starts braking at once and stops within a few seconds The displays and options are the same as described in the previous section Short time centrifugation For short term operation the Biofuge haemo is equip ped with a quick run function This option is available whenever the LED next to the quick run key is lit The preset speed is ignored Centri fuge tubes and rotor may be dam aged Check carefully whether you have to maintain a specific speed Never use this option for hematocrit measurements 34 When you press and hold the quick run key the cen trifuge accelerates with full power up to the maximum speed of 13 000 rpm unless you release the key The preset speed is ignored During acceleration the time is counted forward in sec onds After 60 seconds the display changes to the min ute mode The quick run mode is not recommended for sensitive samples with fluffy sediment RCF value The relative centrifugal force RCF is usually given in multiples of the earth gravity g It is a dimensionless number that allows one to compare the efficiency of separation or sedimentation
37. s Langenselbold Germany Tel 49 0 6184 90 6940 Fax 49 0 6184 90 7474 info de kendro spx com Latin America Kendro Laboratory Products International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 9466 info kendro spx com North America Kendro Laboratory Products Asheville NC USA Tel 1 800 879 7767 Fax 1 828 658 0363 info kendro spx com Internet http www kendro com ___Kendro Laboratory Products GmbH ee el TO Robert Bosch Strafte 1 notice D 63505 Langenselbold i Telephone 49 0 6184 90 6940 20056895 Bio haemo uk 11 03 Printed in Germany
38. sing the selected key or its counterpart if you have proceeded too far in one direction The third digit from the right keeps flashing The speed is stored by again pressing one of the set keys or start 29 Operation Selecting the run time You can select a run time between 1 and 99 min or continuous operation For hematocrit determinations you should select a run time of 10 min for a standard blood column of about 65 mm The evaluation of hematocrit measurements is detailed on page 35 Preselected run time To predetermine the run time proceed as follows 1 Press the set key in the time panel press twice if the centrifuge is in the standby mode The run time last entered appears in the display The right hand digit in the example on the following page the figure 5 flashes thus indicating that you can change the setting in minutes 2 Press one of the set keys increase or de crease By pressing the selected key briefly you increase or decrease the preset run time in steps of 1 min This option is supposed to be used for small changes and fine tuning 3 If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace 30 Li 15 speed time 4 Release the key as soon as you are close to the desired value and fine tune if necessary by repeat edly pressing the selected key or its counterpart if you have proceeded too far The disp
39. stic signal for stopping 32 aerosol tightness check 27 aluminum rotor 39 approved rotor 22 autoclaving 41 autoclaving cycle permissible maximum 41 C capillaries placing 19 centrifuge starting 31 centrifuge tubes 8 cleaning 37 54 conditions of warranty 42 contamination necessary measures 38 continuous operation 30 33 control panel readings 8 corrosive substances protective vessels for 4 D damage symbol for potential 5 dangerous chemicals 3 decontamination 38 density maximum of sample 25 disinfectant 39 disinfection 4 procedure 39 disinfection with bleaching lye 40 display during run 31 displays brief failure 43 not lit 43 E EC Guidelines 5 emergency lid release 18 Emergency lid release 7 error code br 44 Lid 45 OPEN 45 E 1 or E 12 44 E 3 44 F fastening nut for hematocrit rotor 20 fine tuning run time 30 speed setting 29 fixed angle rotor 11 adapters 13 optional accessory 24 technical data 12 fluorescein test solution for aerosol tightness 27 fuses 50 H hazardous substances 3 hazards symbols used for 5 hematocrit measurement 35 standardization 35 hematocrit determination quick run function not to be used 33 hematocrit dish standard equipment 9 hematocrit rotor fastening 20 insertion 19 loading 19 removal 20 technical data 10 hints symbol for 5 l icons for denoting dangers and potential damage 5 infectious material precautions in cas
40. ximum RCF value 16 060 fixed angle rotor 14 926 hematocrit rotor minimum speed 1600 rpm minimum RCF value 209 noise lt 64 dB A maximum capacity 24x2 ml maximum permissible load 24x4g maximum permissible unbalance 1g 50 Technical data Parameter Value description maximum kinetic energy 2 000 kNm compliance with standards Manufactured and checked in accordance with EN 61 010 1 EN 61 010 2 020 EN 50 081 1 EN 50 082 1 Electrical connections fuses Order no Voltage Frequency max current Power consumption Fuses inside instrument 7500 3635 230 V 50 60 Hz 1 25A 170 W 2 x 6 3 AT 5 x 20 mm 7500 3557 120 V 50 60 Hz 2 5A 170 W 2x 6 25 AT 6 3 x 32 mm 1 x 10 AT 6 3 x 32 mm 51 Speed g diagram for rotor 7500 3325 100000 o Oe og TS 13000 ff rae ee FEE ee eua eee Q 10000 o l mes E 1000 N lt Ens a 100 10 100 1000 10000 Y 100000 16060 RZB x g RCF x g 52 Autoclaving protocol only fixed angle rotor 7500 3325 ope e 9 mem 99m 53 Index A acceleration 8 accessories additional 11 standard 9 acou
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