UV Spectrophotometer LUV
Contents
1. Adjust the vertical position of the W lamp to make the light spot falling to the centre of the slit Be careful the lamp is hot e Tighten the two holding screws after adjustment 5 Assemble the upper cover of the lamp housing and the instrument 6 Switch onthe instrument If initialization is correct the replacement of tungsten lamp is finished Note The wiring of tungsten lamp and deuterium lamp should be done properly The holding screws on the wires should be tightened CHAPTER 6 ACCEPTANCE OF THE INSTRUMENT Check the following items after warming up the instrument for 30min 6 1 Wavelength Accuracy 0 3nm Setting parameter in wavelength scanning mode womg J OCS Measure the spectrum of deuterium lamp Use the cursor to observe the maximum value between two characteristic peaks The results should satisfy the requirements of 656 1nm 0 3nm and 486 0nm 0 3nm 6 2 Baseline Flatness 0 002A Setting parameters in wavelength scanning mode Working Mode ee Wavelength Range 200nm 1100nm Photometric Range 0 010A 0 010A Sample and Reference Cells Empty Perform the wavelength scan measurement The maximum deviation of the scanned spectrum should be in accordance with the requirement of the baseline flatness 6 3 100 T noise lt O 5 T 3min at 500nm Set parameters in time scan mode Photometric Range 99 5 T 100 5 T Sample and Reference Cells Empty Perform the kinetic scan2. LCD or printed out 4 2 1 Parameter Setting Inthe WL Scan menu press F1 key to enter the parameter setting menu as shown in Fig 4 4 2 2 PARAMETERS Mode A Scan Speed Fast Interval 1 0nm Cell Position 1 WLFrom 200nm to 1100nm Y Axis From 0 010 to 0 010 Lamp Change 340 0nm Fig 4 Parameter Setting Page in WL Scan Mode There are two types of parameter settings Mode Scan Speed and Interval are Select type parameters while the rest are all Input amp confirm type parameters Please pay attention to the following points in parameter settings A Photometric Range This parameter refers to the coordinate range displayed on the screen and is not related to the measurement If it is selected improperly the spectrum may not be observed The parameter can be set again by Spectrum Zoom function after obtaining the scanned spectrum The concrete method will be described later The photometric measurement range of the instrument is O T 220 T or 0 301Abs 3 000Abs If the setting range is exceeded an error will be displayed Sampling interval Interval For complex spectra smaller sampling intervals should be chosen as large sampling intervals may yield distorted spectra However excessively small sampling intervals will reduce the scan speed and range For scanning the full wavelength range the minimum sampling interval should be 0 5nm Pay attention to select a suitable match of wavelength r
3. T O T 100 T lt 0 15 T 0 002A lt 0 001A h at 500nm after warming up Self Diagnosis Functions The instrument can automatically recognize most of the errors including operation error and gives out prompts or alarms Parameters Can Be Saved The instrument saves the user set parameters automatically The saved parameters will not be lost after the power is turned off Wavelength Scan Measurements WL Scan a Maximum sample collection points 3000 b Maximum scanning speed 1000nm min maximum moving speed 3000nm min c Minimum sampling internal 0 1nm Fixed Wavelength Measurement Mode Photometric Measurement a Maximum of nine wavelengths can be measured at one time Quantitative Measurement Mode Quantitation a Firstand second order standard curve can be established b Concentration direct reading can be made after inputting the curve factors c Providing the analytical methods with single wavelength double wavelengths and triple wavelengths d Protein DNAanalysis can be made using double wavelengths method Kinetics Measurement Mode Time scan a Time Range 0 86400sec Minimum sampling interval 1sec b Maximum sampling points 3000 7 Spectrum Process Kinetics Wavelength scan a Cursor reading b Spectrum Zoom c Spectrum Conversion d Peak Valley Picking e Spectrum printout 8 System Setting Tungsten lamp and deuterium lamp On Off control Wavelength re calibrati
4. before hand activity coefficient can be saved in memory 4 6 System Settings The system setting page is shown in Fig 12 System Setting W Lamp D2 Lamp WL Calib Printer PCL5e Cell Num 8 Serial 00000000 Language English Fig 12 System Settings A Press or gt key to control the tungsten lamp and deuterium lamp On Off B WL Calibration Press or gt key to set wavelength calibration function on and start wavelength calibration Please make sure that the cell holder is empty while performing wavelength calibration C Printer To set the type of the printer Mini means the mini printer which can be order from the manufacturer PCL5e means laser printer with PCL5e communication protocol D Cell Number 8 cell holder and 4 cell long path cell holder can be set as alternatives After changing a cell holder be sure to make necessary setting change here E Serial Number Itis required when connected with PC F Language to set displayed language CHAPTER 5 MAINTENANCE 5 1 Points for Attention 1 Allinstrument systems must be reliably grounded 2 If the stable power system is not provided a regulated power supply with anti interference and purifying capability should be installed to ensure the stable and reliable operation of the instrument 3 All of the components and parts on the whole system should never be removed arbitrarily 4 The instrument should not be cleaned with organic so
5. has not been preformed before when pressing F2 key aprompt Press zero first will appear to ask you to perform baseline correction first Please make sure that the reference solution has been put in both the reference and sample cells when baseline correction is being made 4 2 3 Spectrum Processing A Cursor Function Press key the cursor moves from the left side of the spectrum to the right side and press or gt key to stop and display the corresponding position and data There are two methods of moving the Cursor a Manual press or gt key to move one digital point leftwards or rightwards b Rapid Pressing numeric keys 0 to 4 corresponds to 5 left moving speed levels respectively while numeric keys 5 to 9 corresponds to 5 right moving speed levels respectively Pressing or gt key can stop the cursor from moving at any time B Spectrum Zoom After the wavelength measurement enter the parameter setting page Modify up down limits of Wavelength Range and photometric range The wavelength range must be in the setting range of the measurement Afterwards return to the wavelength scan menu and press or gt key to display the spectrum C Spectrum Conversion A T Enter the parameter setting menu modify the measurement mode and then return to the wavelength scan menu Press or gt key to display the spectrum after A T conversion D Peak Valley Picking 1 Press F3
6. key in the wavelength scan main menu to display peak valley picking page as shown in Fig 5 Peak Pick Fig 5 Pick Valley Picking Page 2 On the top of the Peak Valley Picking page the threshold sensitivity is displayed following current working mode The working mode has been set in parameters page 3 The threshold sensitivity is the smallest difference between peak and valley in each group Threshold sensitivity is the only standard for peak valley detection The threshold should be selected according to user s requirements Selecting excessively small values will yield too many peaks and slow down determination Excessively large thresholds can result in no peaks or valleys detection 4 Press numerical keys to input the threshold value Then press ENTER to confirm The value can have one or three decimal digit according to the spectrum type 5 After inputting the threshold value press F2 key to start peak picking The detected result will be displayed on the screen if the peaks and valleys are detected The result includes peak valley positions and intensity values 6 Four groups of peaks and valleys can be displayed in one page If several peaks valleys are detected a page display at the lower right of the screen such as 2 1 will be indicated The figure before tells the total number of pages while that after shows the number of current page For example 2 1 means there are altogether 2 pages displaying the p
7. LABNICS Equipment DOUBLE BEAM UV VIS SPECTROPHOTOMETER x Jnstruction anual Model LUV 100A L Please read this manual carefully before using the instrument _ Labnics Equipment CHAPTER 1 1 1 1 2 1 3 1 4 2 2 1 2 2 2 3 3 4 4 1 4 2 4 2 1 4 2 2 4 2 3 4 2 4 4 3 4 3 1 4 3 2 4 4 4 4 1 4 4 2 4 4 3 4 5 4 5 1 4 5 2 4 5 3 4 6 5 5 1 5 2 5 3 5 3 1 5 3 2 6 6 1 6 2 6 3 6 4 e Table of Content e CONTENT Preface Installation Unpacking Site Requirements Installation Inspection After Installation Specifications and Main Functions Basic Parameters of Instrument Main Specifications Main Functions Structure Operation Main Menu Wavelength Scanning Measurement Mode Parameter Setting Scanning Measurements Spectrum Processing Printing Photometric Measurement Mode Parameter Setting Measurement Running Quantitative Measurement Quantitation Establishing the Standard Curve Concentration Measurement Deleting the Set Standards Time Scan Mode Kinetic Measurement Mode Parameter Setting Scan Measurement Activity Calculations System Settings Maintenance Points for Attention Measures Taken When Operation of the Instruments is Abnormal Replacing Lamps Replacement of Deuterium Lamp Replacement of Tungsten Lamp Acceptance of the Instrument Wavelength Accuracy Baseline Flatness 100 T noise lt O 5 T 3min at 500nm O T Noise lt O 2 T 3min Appendix A Se
8. Remove the two screws for D2 lamp replacement Fig 13 Lamp Housing 3 Detach the upper cover of the lamp housing Remove the two screws located on the upper cover of the lamp housing Be careful Don t drop the screws into the instrument It is not easy to retrieve them Then take off the upper cover of the lamp housing The positions of D2 lamp and W lamp are shown in Fig 13 4 Replace the deuterium lamp Detach the D2 lamp lead wire plug e Remove the two holding screws of D2 lamp and then remove the D2 lamp e Replace a new deuterium lamp onto the holder e Tighten the two screws to hold the lamp properly e Plug inthe D2 lamp lead wire plug 5 Assemble the upper cover of the lamp housing and the instrument 6 Switch on the instrument If initialization is correct the replacement of the deuterium lamp is finished 5 3 2 Replacement of Tungsten Lamp 1 Set the wavelength at 500nm Perform a photometric measurement at 500nm Switch off the instrument Unplug the power cord 2 Remove the upper cover of the instrument as described in Section 5 3 1 3 Remove the upper cover of the lamp housing as described in Section 5 3 1 4 Replace the tungsten lamp according the following procedures refer to Fig 5 Detach the W lamp lead wire plug e Loosen the two holding screws of W lamp and then remove the W lamp Replace a new W lamp onto the holder e Plug in the W lamp lead wire plug Turn on the instrument
9. and different sample batches a new standard working curve should be set up The user should decide if it is necessary to set up a new curve according to the specific application 4 4 1 Establishing the Standard Curve There are two methods of establishing standard curves Parameters Method 1 WL K1 Value 0 000 K2 Value 0 000 B Value 0 000 Lamp Change 340 0 WLO 440 0 C K1 A A K2 A B Fig 9 Parameter Setting Page in Quantitation Mode A Inputting the factors of the standard curve a b Press F1 key to enter parameter setting page as shown in Fig 9 There are two types of parameters Select type Cell Corr and Method The rest parameters are Input amp Confirm type Input the factors in parameter setting page according to the known curve formula C K1 A A K2 A B Among them K1 is the factor of the quadratic term K2 is the factor of the first term Bis the constant term or the intercept When K1 is zero the curve is a linear one Then input the operating wavelength The inputting value will be displayed at the upper right of the screen Press ENTER to confirm Press RETURN key to return to the quantitation main menu B Setupthe standard curve by measuring standard samples This method can be performed by first order or second order data regression with up to 24 standard samples a Press F3 in the quantitation main menu to enter the standards measurement page as shown in Fig 10 Pre
10. ange and sampling interval There are six options for sampling intervals setting 0 1nm 0 2nm 0 5nm 1 0nm 2 0nm and 5 0nm Lamp changeover point Lamp Change Lamp changeover wavelength can be set within the entire wavelength range Normally lamp change wavelength should be at 340 nm when measuring the absorbance or the transmittance After setting the parameters press RETURN key to enter the wavelength scan main menu Scanning Measurements After setting the parameters put the reference solution into both reference and the sample cells and close the sample compartment door Press ZERO key to make the baseline correction As complicated sampling computation will be made by the instrument during the baseline calibration please wait until baseline prompt disappears That shows the baseline correction has been finished After completing the baseline correction take out the reference solution from the sample cells and then put the sample solution to be determined into the sample cell position close the sample compartment well press F2 key to start measurement If the user wants to stop the scan during measurement running press CE key and return to the wavelength scan main menu At this moment the instrument is making computation So the key response may be delayed itis anormal case After the measurement dynamic display stops the moment spectrum processing can be performed NOTE If the baseline measurement ZERO
11. ate the cursor at the No of cells in which you want to put the samples then press ENTER key to select A y symbol will appear at the cell No to show that the cell is selected Put the reference in both sample and the reference cells and then press ZERO key to start baseline measurement After baseline measurement is completed put the sample to be determined in the sample and then press F2 key to start concentration measurement 4 4 3 Deleting the Set Standards In the quantitation main menu press F3 key then F1 key to enter concentration inputting page To clear all the standards press CE key The system will ask you to confirm deleting Press ENTER to confirm Now Press CE to cancel If you want to delete a certain standard locate the cursor at it and then press ENTER to delete or recover the data of this standard 4 5 Kinetics Measurements Mode Time Scan Measurement Mode This mode is used to observe a sample s variation with time and to calculate the activity value of the sample in the selected time period It is also used to check the stability and the noise of the instrument 4 5 1 Parameter Setting a Press F1 key to enter the parameter setting menu the time scan parameters are similar to the wavelength scan parameters There are only a few changes wavelength range changes to time range the sampling interval changes to Input amp Confirm type and adding the measuring wavelength Meas WL item The w
12. avelength is an Input amp Confirm type parameter b Inparameter setting attention should be paid to the following The measuring time should well match the sampling interval For longer measuring time larger sampling intervals should be used If they do not match an error message will appear on the screen The user will need to set them again c After parameter setting press RETURN key to return to the kinetics main menu 4 5 2 Scan Measurement Refer to Section 4 2 2 to start scan measurement After completing the measurement perform spectrum processing as described in Section 4 2 3 CAUTION The newly scanned time spectrum will overwrite the original one whether it is a time spectrum ora wavelength spectrum No warning will be displayed before overwriting 4 5 3 Activity Calculation Press F3 key to enter the Spectrum Process page after completing the measurement select the activity calculation to enter the activity calculation page as shown in Fig 11 Activity Calculation Time From To Coefficient Result Fig 11 Activity Calculation Menu Time Range and Activity Coefficient are all Input amp Confirm type parameters After parameters are set press F2 key to obtain activity value press F4 key to print out parameters and measured results CAUTION During the activity calculation new results will overwrite the old As there is no Save Function please print out or write them down
13. d data printing etc e Parameters can be saved for a long time after turning off the instrument Spectrum and data can be stored when sudden power failure occurs Spectrum and data can be sent to computer via RS 232 interface CHAPTER 1 INSTALLATION Please read the instructions in this chapter carefully before unpacking and installing the instrument 1 1 Unpacking While unpacking please check all parts of the instrument for damage according to the packing list If any parts of equipment are missing or damaged please contact the local dealer immediately 1 2 Site Requirements The instrument should be installed in a place that satisfies the following requirements 1 Avoid direct sunlight and ensure adequate ventilation 2 Do not install the instrument where there may be corrosive gas or excessive dust 3 Avoid the place where strong and sustained vibrations occur 4 Keep away from devices that generate magnetic or electric fields or emit high frequency electromagnetic waves The instrument should be placed on a stable horizontal weight bearable workbench The back of the instrument should be at least 15cm away from the wall to ensure the effective ventilation 5 Keep away from high temperature and high humidity environments 6 Room Temperature 5 C 35 C 7 Room Humidity 85 8 Power supply AC voltage 220V 22V 110V 10V Frequency 50Hz 1Hz 60Hz 1Hz Total power consumption 200W 9 Power
14. d of D lamp ignition 3 The system enter the initialization page after igniting the lamps initialization starts automatically as shown in Fig 1 Initializing W Lamp D2 Lamp Filter Lamp Position Cell Position WL Position Fig 1 Initialization Page The initialization result is displayed after completing the initialization If the initialization runs smoothly OK will be displayed at each item Press any key except RESET key to enter the Main Menu Ifa problem is encountered during the initialization ERR will be shown Press RESET key to make the initialization again If ERR persists after several initialization attempts please contact your local dealer for service Refer to Chapter 4 for the operations of the instrument in details 4 Each time you turn on the instrument allow the instrument to warm up for 10 30 minutes after initialisation CHAPTER 2 SPECIFICATIONS AND MAIN FUNCTIONS 2 1 Basic Parameters of Instrument 2 2 2 3 Wavelength Range Monochromator Transmittance Range T Absorbance Range Abs Spectral Bandwidth Minimum Sampling Interval Energy Range Main Specifications Wavelength Accuracy Wavelength Reproducibility Stray Light Photometric Accuracy Photometric Reproducibility Baseline Flatness Stability Main Functions 190 1100nm Plane Grating O T 220 T 0 301A 3 000A 2nm 0 1nm 0 000V 9 999V 0 3nm lt 0 15nm 0 1 T Nal at 220nm 0 3
15. e with a new motor or optical coupler Contact the local dealer Wavelength initialization error The light is blocked in the Remove the light block from sample cell the sample cell The sample compartment door Don t open the sample was opened during the compartment door during initialization initialization The wavelength shift is too large Contact the local dealer or space deviation exceeds the limitation Photometric accuracy and The absorbance value of the Dilute the sample solution reproducibility are not sample is too big gt 2A satisfactory Glass cell is used in UV range Use quartz cell for UV range The cell is not clean enough Clean the cell The sample cell holder is dirty Clean the sample cell holder etc Contact the service engineer Energy is too low appears Alight block is in the sample cell Remove the blocking element A prompt ER appears Glass cells are used in UV range Use quartz cells in UV range below 350nm Clean the cells The cell is not clean enough Set lamp changeover point Lamp change wavelength is set between 340 360nm wrongly 5 3 Replacing Lamps The Deuterium and tungsten lamps are consumable parts having finite lifetime If a lamp s operating time has exceeded the normal operating period the light energy and lamp stability will deteriorate even if it is still functioning If the stability and reproducibility of the instrument dec
16. eak picking results and the current page is the first one If there are several pages of peak valley data press or key to scroll up and down 7 Press F4 Print key to print the peak valley picking result 8 Press RETURN key to return to the wavelength scan main menu 4 2 4 Printing Press F4 key to enter the printing state on the wavelength scan page A prompt line Printing appears on the upper right of the screen If ERR is displayed a problem may occur in the printer or the printer has not been properly connected To cancel printing press CE key Because the system is busy key response may be slower than usual If the printing is abnormal or no spectrum gets prints out please verify if the printer selected in system settings page is in accordance with the printer connected NOTE Due to Limitation of the instrument s storage ability only one spectrum can be stored in the memory Therefore when a new wavelength scan measurement is made the original spectrum will be deleted 4 3 Photometric Measurement Mode Press the numeric key 2 in the main menu to enter the Photometric Measurement mode Fig 6 Multi samples at multi wavelengths can be measured at the same time in this mode The maximum wavelength that can be monitored is nine Each wavelength corresponds to each sample Photometric Cell T 1 2 WL 235 0 Fig 6 Main Menu of Photometric Measurement Mode 4 3 1 4 3 1 Parameter Se
17. for 180 seconds the maximum peak to peak value of the spectrum should satisfy the stipulated requirement Itis 100 T noise of the instrument 6 4 O T Noise lt O 2 T 3min Insert the light block into the sample optical path Change the photometric range to O T 0 3 T The rest parameters should remain the same with item 3 as described above The value determined will be 0 T noise It should satisfy the stipulated requirement Appendix A Reference Table of Terms and Abbreviations Energy normally energy of light source used only when accepting the instrument E Wavelength unit nm Series Interface port Used to communicate with computer COM1 must be used Spectrum bandwidth In theory monochromatic light is required when carrying out material analysis In practice the instrument cannot produce pure monochromatic light Instead it produces light in a wavelength range with certain wavelengths at the centre The width of the range is the spectrum bandwidth The smaller the spectrum bandwidth the higher the analysis accuracy For smaller bandwidths though stability and reproducibility can deteriorate If there is no special requirement otherwise a wider spectrum bandwidth is suggested to ensure higher stability and reproducibility Standard Curve The standard curve shows the corresponding relationship between the Regression Curve measured absorbance and concentration The standard curve can be set up by measuring the ab
18. lvents 5 Pay attention to the instrument in areas of high humidity and temperature The instruments should not used for along time in such climatic conditions as should be turned on periodically for drying 6 LCD Screen if get damaged once there is no way to repair it so be careful while using and don t extrude and impact it 7 If the measurement is made only in the visible range above 350nm the deuterium lamp should be turned off in System Settings page after completing the initialization of the instrument in order to extend its using lifetime 8 To improve the operating performance of the instrument the manufacturer can make some modifications on the instruments There may be slight differences between the instrument descriptions and the actual instrument Users will not be notified of these modifications 9 The instrument is of Class safety is designed and tested according to the JB5517 91 Basic safety requirements for electrical protection of optical instruments The supplied products are guaranteed for their safety To ensure the safe operation users must comply with the instructions and items for attention included in this Operating Instruction 5 2 Measures Taken When the Operation of Instrument is Abnormal When the instrument is in an abnormal state during operation the user should carefully observe its behavior and take a note of it for later use and immediately contact service engineer 1 When an error
19. on Printer type selection Sample cell holder selection Inputting the serial No of the instrument Language selection gt 9 A200 CHAPTER 3 STRUCTURE Model LUV 100A Spectrophotometer consists of light source monochromator sample compartment detecting system motor control LCD display keyboard power supply micro processor RS232 interface and printer port The block diagram of the instrument is shown in Fig 2 Keyboard Display Printer Port RS232 Interface Main Control Fig 2 Block Diagram of Instrument CHAPTER 4 OPERATION Before the operation please pay attention to the following points 1 Operation prompt If a prompt question appears on the screen press CE key to give a negative answer or press ENTER key to give a positive answer 2 After entering the function operation make sure not to press the keys unnecessarily as this may cause malfunction of the instrument 3 During measurement running don t compress the instrument while the sample compartment door must remain closed 4 Inthe sample compartment the reference cell and the sample cell should be placed at 90 angle directions referring to Appendix C Pay great attention to the direction of light passing sides of the cells when placing the cells into the cell holder NOTE The rules of data input are Only one decimal digit is permitted for wavelength value T T value and sampling interval Maximum of three decimal digi
20. prompt appears on the screen of the instrument carefully check if there is something wrong with the operating method the parameter settings are well matched the samples are correct and the lamp is ignited etc If there is no confirmed operating or setting error attempt operation once again If the error persists contact the vendor for further assistance 2 When errors in computer programs are discovered or the system crashes please check if the installing conditions are correctly followed as described in the operating manual If the problem persists contact the vendor or a qualified service engineer 3 If there is something wrong with the instrument during operation first refer to the following troubleshooting table If the problem cannot be corrected please contact the vendor or a qualified service engineer Table 1 Error id PossibleCause Solution __ _ _ No response when switching The plug may loose Connect the plug well on the instrument The fuse may be broken Replace a new fuse W D lamp error during The W D lamp is damaged Replace with a new W D lamp initialization There is something wrong with Contact a service engineer the power supply circuit of W D lamp Error of lamp location filter The plug may loose Check every plugs and or sample cell The motor may be damaged connections inside instrument The optical coupler does not and make sure they are work connected well Replac
21. reases and this cannot be traced to vibration unstable power supply or other reasons listed in Table 5 1 a lamp change is probably necessary The following points should be observed when replacing lamps Ai 2 wo When removing the upper cover of the instrument avoid not pulling the connecting cable on the cover with force Do nottouch the optical parts inside the instrument If screws drop into the instrument take them out immediately to avoid short circuits During lamp installation do not touch the lamp window Fingerprints can contaminate the lamp and lower the output energy Lamps can be cleaned with alcohol soaked absorbent cotton and then dried with dry absorbent cotton When installing the upper cover of the lamp housing and upper cover of the instrument make sure the connecting cable of the instrument is not compressed or impeded The lamp wiring should be reliably connected and secured otherwise the performance of the instrument will be affected 5 3 1 Replacement of Deuterium Lamp 1 Switch off the instrument and remove the power cord 2 Remove the upper cover of the instrument according to the following procedure l Loosen and remove the three screws located at back of the instrument upper cover Il Lift the instrument cover with care and put it at the left side of the instrument You can see the lamp housing as shown in Fig 13 Loosen the two screws on the W lamp holder for W lamp replacement
22. rvice Report PAGE No 1 OMOWAONDAAAARPRPWWWWNHNHND PREFACE The LUV 100A is a double beam UV VIS spectrophotometer with scan function It is widely used in medicine environmental monitoring commodity inspection food inspection agricultural chemistry teachingyin colleges and universities metallurgy geology machine manufacturing and petrochemical industries It is also a helpful tool for analysts to carry out qualitative and quantitative analysis of materials The main features are as follows 1 Fully Automated Operations automatic change over between W lamp and D lamp automatic filter changing automatic wavelength calibration auto controlling W lamp and D lamp On Off automatic zero and 100 T adjustment W lamp and D2 lamp provided with holders easy replacement of W lamp and D2 lamp Friendly interface abundant operation prompts convenient and fast operations Blue LCD display module with 320 x 240 large screen Embedded single chip micro processor control system Rich and powerful functions Main Measurement Modes WL scan A T E Photometric measurement Fixed wavelength measurement A T Quantitation Quantitative Measurement A C Time scan Kinetics Measurement A T OX OT EAO Powerful Spectrum Processing Functions Cursor moving and reading Spectrum zoom Spectrum conversion A T Peak valley picking Activity calculation Spectrum printing e Data Processing Functions data looking up an
23. sorbance of standard samples of known concentration normally the absorbance values of the standards should be less than 1A or entering the curve factors directly Generally concentration values of samples to be measured should be in the range of the standards The standard user should be distributed evenly across the entire concentration range Appendix B LUV 100A Sample Compartment Descriptions Direction of optical path E ES F Bs 5 e BS Bs Reference SERVICE REPORT Customer s Address Contact Person Designation Date Configuration Serial No Validation QO Courtesy Nature of Problem Observation amp Action Taken Customer s Remarks Parts Replaced Parts Recommended Action Required Yes U NoQ Requisition Number Service Engineer s Name amp Signature Customer s Name Signature Date amp Stamp Page Of Tel No Fax No Weekly Off Dept Date SR No Status OK NotOK Installation Q Warranty Demonstration Q Maintenance Contract Repairs QO Application Billable Calibration Q O O O OJD
24. ss W or key to locate the cursor at the No of cells in which you want to put the standards then press ENTER key to select A V symbol will appear at the cell No to show that the cell is selected Put the standard samples in the corresponding cells and put the reference in cell No 8 then press F2 key to start absorbance measurement The system will calculate the standard number automatically according to the measurementtimes D Std Data Cell A0 A0 A1 21 A2 i2 Conc 1 Data Mea Regr Prn Fig 10 Standards Measurement Page b After measuring the absorbance of the standards press F1 key to enter concentration inputting page to input the concentration of the standards Press V or key to locate the cursor at the No of cells in which you want to put the standards then press ENTER key to select A V symbol will appear at the cell No to show that the cell is selected Press gt key to move the cursor at Conc column and input the known concentration of each standards The inputting value will be displayed at the upper right of the screen Press ENTER key to confirm c Press F3 key to select regression order First and second regression orders can be selected by pressing lt or gt key Then press F2 key to get the standard curve d Press RETURN key to return to the quantitation main menu 4 4 2 Concentration Measurement In the quantitation main menu press V or N key to loc
25. supply system required by the instrument should be a three phase and four line zeroing protection system 10 To ensure the normal and reliable operation of the instrument it is better to use a purified regulated power supply 1 3 Installation 1 After unpacking place the instrument on the workbench 2 Connecting external cables A Connect the printer interface cable to the corresponding socket on the main unit if a printer is equipped B Connect the power cables of the main unit and the printer to an AC outlet 1 4 Inspection after Installation WARNING 1 In very cold areas the instrument should be left alone for 8 hours before turning on the power to ensure that there is no moisture or condensation inside the instrument and a temperature balance with the room temperature should be achieved Before turning on the instrument examine all cables to ensure correct and secure connections the compliance of the power supply with the requirements and the security of the earthing of the whole system The instrument can be turned on and operated when these requirements have been satisfied 1 Turn on the printer If you want to print For the mini printer just connect the power cord 2 Turn on the power of instrument the power switch is at the right side of the instrument The tungsten lamp and the deuterium lamp are ignited in sequence firstly ignites W lamp after 10 sec D lamp is ignited You can hear the click soun
26. ts are permitted for curve factors K and B absorbance A concentration C and activity coefficient Ifa parameter is a range value input it in the sequence from small to large NOTE Select type parameter measurement mode scan speed wavelength sampling interval switches of W lamp and D2lamp etc Move the cursor to the corresponding item by pressing the V and keys and select via and gt arrow keys If V is used for selecting the items press ENTER key to select or to delete Input amp Confirm type parameter wavelength range photometric range Lamp Changeover point etc Move the cursor to the corresponding item by pressing the V and keys Input the data which will be displayed on the upper right of the screen If the entry is incorrect press CE key to cancel Otherwise press ENTER to confirm The newly input data will replace the original data Input type WL Num Std Num Move the cursor to the corresponding item by pressing the V and A keys And then input the data 4 1 Main Menu The main menu is shown in Fig 3 Press the corresponding numerical keys to enter different operating modes Main Menu WL Scan Photometric Quantitation Kinetics System Settings Fig 3 Main Menu 4 2 Wavelength Scanning Measurement Mode Wavelength scanning measurement mode can be used for the qualitative analysis of samples The real time spectrum of samples will be displayed on
27. tting Press the numeric keys to set the cell numbers and the wavelength numbers The wavelength is input amp Confirm type parameter If multi wavelengths determination is made it is recommended the wavelength values be set in the sequence from small to large After parameter setting press RETURN key to return Parameters CellNum 7 Lamp Change 340 0 WLNum 4 WL 1 235 0 WL 2 250 0 WL 3 400 0 WL 4 500 0 Fig 7 Parameter Setting Page in Photometric Measurement Mode 4 3 2 Measurement Running After setting the parameters put the reference solution into both reference and the sample cells press ZERO key to make the baseline correction After the baseline correction is finished put the sample to be determined into the sample cell close the sample compartment Then press F2 key to start the measurement After the measurement press the or gt key to look up the measurement results of each wavelength or press F4 key to print out the data 4 4 Quantitative Measurement Quantitation Press 3 key to enter the quantitative measurement menu as shown in Fig 4 6 Quantitation Cell A0 A0 A1 21 A2 22 Conc 1 Fig 8 Quantitative Measurement Menu Before quantitative measurement the standard working curve of absorbance A and concentration C C K1 A A K2 A B should be set up After a standard curve is set up the instrument will save the curve formula automatically For different kinds of samples
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