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1. 300 2 Thermo Electron Laboratory Products Humidity Simultaneously depress SET TEMP key and RH key The alphanumeric display will 4 7 mn show VIEW H OFS d ri vow serco onewe sore AV CO Zero You must perform a CO Zeroing procedure upon initial startup of the incubator Method 1 Auto zero Step 1 Turn the incubator on and set the operating temperature Open the glass door to 45 or more This disables the CO injection solenoid and prevents any accidental discharge of gas while the zeroing procedure is taking place Step 2 Press the CALIBRATE key and the alphanumeric display will show AUTO ZERO and the CO display will blank out The glass door must remain open for at least one minute 8 CO2 Y STATUS LL zi cI after key is pressed Step 3 When the one minute period has E elapsed the alphanumeric message 31 changes to CLOSE DOOR a EE el a ANY C02 open it again before the auto zero process is finished you will see the ZERO ERROR message on the a
2. A il e i efer Od Ad oar UE a u ONIGIA SINAIS 005 30 ONISIA SALAS 00 34113 AH ADS AS USER LO y ae ET ESE aad BN IA 2 ea feb gt La Am Os JOH EER ag PA CASITA HU ETEND NA L NV D OTEA A ARO 2 NU Y q3 oe AAANVH dul DVAZYZ BET GEL S6 DNA FOWL TDA INNI J ONIS CAIN LAAIVCAILVM 31800 700 20 ES 00 a Full Featured Water Jacketed amp Radiant Wall CO2 Incubators Looking for more information bh Artisan Visit us on the web at http www artisan scientific com for more information QUALITY INSTRUMENTATION GUARANTEED Price Quotations Drivers Technical Specifications Manuals and Documentation Artisan Scientific is Your Source for Quality New and Certified Used Pre owned Equipment e Tens of Thousands of In Stock Items Fast Shipping and Delivery Equipment Demos Hundreds of Manufacturers Supported Leasing Monthly Rentals Consignment Service Center Repairs InstraView Remote Inspection Experienced Engineers and Technicians on staff in our Remotely inspect equipment before purchasing with our State of the art Full Service In House Service Center Facility Innovative InstraView website at http www instraview com We buy used equipment We also offer credit for Buy Backs and Trade Ins Sell your excess underutilized and idle used equipment Contact one of our Customer Service Representatives toda
3. 8 On for 8 segment alphanumeric displays Off for 10 segment displays see p 700 5 EF NOTE Remember that a top deck will not actually have a Tank Switcher installed but DIP switch 7 should be ON so the proper alphanumeric messages will be displayed 501 2 Unplug the Door Heater harness from Power Board connector JP1 and measure the resistance should measure 130 188 ohms 220V units 240 300 ohms 501 3 Re connect harness to JP1 501 4 Unplug Chamber Heater harness from Power Board connector JP4 and measure the resistance should measure 40 80 ohms 220V units 165 280 ohms 501 5 Re connect harness to JP4 501 6 Open the outer and glass doors Apply Power 501 6 1 Display Board should light up 501 6 2 The two LED s on the power board should be lighted 501 6 3 Close the glass door and observe that the DOOR OPEN warning extinguishes and that the chamber fan begins to turn 501 6 4 If the water jacket level is low observe that the ADD WATER warning extinguishes when sufficient water has been added through the Fill Port 500 1 Thermo Electron Laboratory Products 501 7 Verify Control Voltages CAUTION Use a 4 digit voltmeter for these checks 501 7 1 Place the red probe on TP1 on the 31664H01 Main Control Board and the black probe on signal ground on the 31666H01 Power Board The voltage should read 4 000 0 003 i e 3 997 to 4 003 501 7 2 Adjust R31 on the Main Control Board if the voltage is o
4. The water jacket temperature probe should read the correct temperature within 0 1 C If this is not the case you will remedy the problem by introducing a temperature offset at the display panel as follows 503 1 Place the tip of a thermocouple halfway wads ea tes NR between top and bottom of the water jacket by inserting it through the Fill Port and besa re ee allowing the thermocouple to stabilize three ef 3 to five 5 minutes 503 2 Simultaneously depress SET TEMP SET SSR OVERTEMP and SET CO2 observe that a decimal point to the extreme left in the CO2 display illuminates the unit is now in the SES System Calibration mode oe 503 3 Depress the CALIBRATE key three times Control the CAL WATER message will be displayed ua 3 9 Chamber and the temperature display now showing O A Indicator the water jacket temperature will blink 503 4 While the display is blinking press the UP or DOWN key until the display indicates a temperature of 0 5 C hotter than the temperature measured in Step 503 1 above 503 5 System will automatically exit the Calibration mode if no keys are depressed for approximately fifteen seconds and exit the System Calibration mode in approximately ten minutes 503 6 The system will now equilibrate based on the new value Remove thermocouple used in step 503 1 and resume normal operation 503 7 Remove any existing condensation and allow unit to stabilize overnight evaluat
5. 700 1 700 2 700 5 700 6 700 7 700 8 700 9 700 10 700 12 700 14 700 17 700 19 700 19 Thermo Electron Laboratory Products I RS 232 COMMUNICATIONS e RS232 Communication Between an Incubator and a PC If a communications port option has been ordered with the incubator a diskette containing a file entitled RS232 README DOC is included in the shipment e Requirements In order to run this program you require an IBM XT AT 386 486 or compatible with at least one floppy drive and a color or a monochrome monitor The computer must also have one or two unused COM ports Only Com 1 Com 2 Com 3 and Com 4 can be selected in the program To print the screen Lpt 1 or Lpt 2 may be used A hard disk drive is recommended The program files may use up to 300K of disk space including the two data files which are saved to disk DB9 connections are as follows On the incubator side Pin 2 is transmit data Pin 3 is receive data and pin 5 is ground GND The computer serial COM port must have the following connections in order for communication to function Pin 2 receive data RXD Pin 3 transmit data TXD and pin 5 on DB9 or Pin 7 on DB 25 ground GND You may print as many copies of this file as you like Program description To install the contents of these files on a hard disk you may choose to create a subdirectory with a name of your choice or you may copy the contents of the disk to
6. 20 70 mV 1 00 V Table 2 PARTS LIST Power PUB OE OE N N N N 31666H01 CPU PCB ss SERS DEEG dias 31664G05 Display PEB siese REED ARE DES SEG GE ed 31665H01 Water Jacket Control Sensor oooooo 302079H01 Chamber Display Sensor 302079H02 Infrared CO2 Sensor PCB matched pair 301935G01 Thermal Conductivity Sensor 31853G01 Chamber Heer osse is 300811H01 Door Heater ees esse ee ee ee ee se ee ee ee ee 86790H01 Fan MOLOE sies GE GE EE SEE GE DEEG EE id 21487H01 120V Fan MOOT SeSe GEES se GEED EE EDE DEEG DES EER Ede 2006HO1 240V Blower Wheel EE ER DE GR 86832H01 CO2 Solenoid ValVe ees sesse ke ee see ee 32541G01 before 5 1 98 CO2 Solenoid Valve cccc cece eee ee see ees 33862G02 after 5 1 98 700 19 Thermo Electron Laboratory Products IX SYSTEM ELECTRICAL DIAGRAM as SAIA3S 006 EI cms mie Sef ZD AN 1 aes a i MEE ID AOI E CMILAO TIT EE WLS 1 Ee ee Ga etal ALIOINH TA TWA 430 23H a r SS WIS 8 ES i ae TES AMOS kg H t Tp i KI 12 SHIN REID S Kus Et ODE SP A PS ONTAIA SILES 005 th Heel 80 ONISIA SINAIS 00 a AHI 4 MIE 3E Ed HEB HO E sat at RE EE mo Cr COSSIO HHI OSTDEIND INI JAIA SIIJENIIS 7 Jid 2020 NIE NO aas A ANIE a y S31935 OOE S wz ro wwo ale S SE EL La Ed a j r STO E Jf f Ba EE z Si
7. Allow temperature to stabilize to within 0 2c of setpoint approximately 30 45 minutes and use Fyrite to verify 0 0 CO2 inside the chamber Remove the control housing cover or display panel see Appendix and locate the IR CO2 PCB Remove jumper from JP5 and place it across the two pins of JP4 and momentarily short the two pins of JP1 with another jumper or the tip of a screwdriver If the CO2 display is gt 0 0 unplug the door switch from JP13 and press the front panel CALIBRATE key to begin the Auto Zero procedure observe AUTO ZERO is indicated on the Status display Reconnect JP13 when the status display indicates CLOSE DOOR NOTE The Auto Zero function will last for five 5 to thirty 30 minutes and will be complete when the Auto Zero message is no longer displayed and the CO window resumes displaying the CO content NOTE If the door is closed before CLOSE DOOR message is displayed or if the door is opened before the Auto Zero is completed a ZERO ERROR message will appear and the Auto Zero is aborted To clear the message press CALIBRATE key Remove the jumper from JP4 and place it across the pins of JP3 and momentarily short the two pins of JP1 with another jumper or the tip of a screwdriver Then press the Up or Down button to as required to achieve an output signal of 0 945 Vdc between Common 3 and Vo 2 Press the IR PCB s Up or Down button until the CO display indicates 19 0 Remove jumper from JP3 and store it on one
8. N will be displayed For the first time you run the program answer to this question should be a yes Y Type Y to respond to the question A second screen will pop up which will request data entry The first field requests you to enter intervals for the program to save data to disk The shortest interval for saving data to disk is 1 min seconds The minimum recommended interval for logging data to disk is 5 minutes since incubator temperature and CO variation intervals are typically greater than this Data is saved to disk in ASCII format To view the data any ASCII editor may be used The data may also be imported into a spreadsheet or a word processor as an ASCII file The first field of a data record is the date second field is time a 24 hour clock based on the computer clock third record field is the CO reading and the fourth record field is the CO setpoint The fifth field is the temperature reading sixth field is the temperature setpoint the seventh field is the temperature alarm setpoint the eighth filed is blank if RH option is not present and the ninth field is the record t count Count will increase from 1 to 4000 When count increases to 4000 a new file is created called lastlog dat and the 1 to 4000 records are transferred to the new file lastlog dat New data will continue to be logged under the filename that was initially entered during setup When the data record count increases to 4000 the data in lastlog dat is replaced by da
9. an existing subdirectory To use the install utility provided with the disk place the RS232 data acquisition disk in drive A At the dos prompt type install The quotation marks should not be typed A subdirectory called DATALOG is created and the three files INSTALL BAT INCLOG EXE and README DOC and README BAT are copied to the subdirectory called DATALOG The install utility will automatically place you in the subdirectory DATALOG By executing the DOS command DIR while in the subdirectory DATALOG you will notice the three files You may use an ASCII text editor to view or print additional copies of the Readme doc file If you chose to create your own subdirectory you may do so and then use the copy command to copy to files the three files on the RS232 data acquisition disk to the directory you created The program INCLOG EXE acquires data from the incubator using the RS232 serial communication The communication is established at 9600 bps no parity and one stop bit A standard RS232 DB9 at the back of the incubator is used for connecting the computer to the incubator Cable length between the incubator and the computer must be kept as short as possible Maximum cable length will vary as a function of the quality of the cable 700 2 Thermo Electron Laboratory Products To begin RS232 data acquisition at the DOS prompt type INCLOG in upper or lower case A setup screen will pop up The prompt Modify these settings Y
10. the CO setting must then be changed to 1 5 and allow the incubator to stabilize at 1 5 Introduce an offset which matches the CO offset value at 5 To view the CO offset at 1 5 press the Set CO key and the SET TEMP key simultaneously The two offsets should be within 0 1 of each other To use a setpoint of 7 5 on an IR based unit you must follow the procedure described above except the setpoints that you need to use will be 5 and 10 Set the offset at 10 and use the same offset value at 5 After following the procedure above you may then set the incubator at your desired setpoint To use a setpoint of 12 5 on an IR based unit you must follow the procedure described above except the setpoints that you must use will be 10 and 15 Set the offset at 15 and use the same offset value at 10 After following the above procedure you may then set the incubator at your desired setpoint 700 13 Thermo Electron Laboratory Products VI 1994 TEMPERATURE UPGRADE INSTRUCTIONS Install Thermocouple Step 1 Step 2 Step 3 Step 4 Carefully slide thermocouple between door and gasket into center air of chamber NOTE Silicone gasket tears easily Allow 1 minute for thermocouple to stabilize Record thermocouple and display temperatures Carefully slide thermocouple out of interior chamber and install into water jacket fill port approximately 24 Allow minute for thermocouple to stabilize and record t
11. the hotter the door Indicator LED D8 is lit if the 12V dc supply is functioning properly Indicator LED D9 is lit if the 5V dc supply is functioning properly e Power Board Pin Outs JP1 Door Heater Output JP2 Fan output 1 Line 2 switched by triac Q2 1 Line 2 2 Line 1 2 Line 1 switched by relay K1 3 n c 4 n e JP3 Strip Heater Output line voltage JP4 Main Heater Output line voltage 1 Line 1 1 n c 2 n c 2 Line 2 switched by Triac O1 3 Line 2 3 Line 1 JP5 AC Power Input 1 Line 1 2 Line 2 JP6 Power Interface 1 amp 2 Ground 3 12Vdc to CO2 Solenoid 4 Switched Ground to Door Heater 5 Switched Ground to Main Heater 6 Switched Ground to Fan Relay K1 7 12Vdc to Tank Switching Solenoid 8 5 volts to Control Board 9 12 volts to Control Board 10 12 volts to Control Board 600 4 Thermo Electron Laboratory Products THC Board 33686H01 description and Pin Outs The THC board has humidity 0 1 V and CO 300 mV 1 300V outputs J1 Humidity Sensor Input J2 T C CO Sensor Input 1 Shield 2 G 3 G 4 B J3 Power Input J4 Temperature Signal Input 1 n c 1 Signal 2 12 volts 2 n c 3 n c 3 Ground 4 5 volts 5 Shield J5 CO Output to control board J6 Humidity Output to control board 1 Signal 50 mV per 1 percent CO2 1 Signal 0 1 Vdc for 0 to 2 Ground 100 RH 10mV 1 2 Ground J101 T C Signal Monitor riot w
12. the two rear corners to clear the access plates and pull cover rearward and up 401 7 Unplug the sensor from the Main Control PCB connector JP6 and withdraw it from the cabinet 5 NOTE When replacing a probe in the Lower chamber of a dual chamber model withdrawal and replacement of the sensor will require removing the wiring chase from the rear exterior of the chamber in order to access the sensor harness 401 8 Install the new sensor in the reverse order sealing the opening of the stainless steel sheath with silicone to prevent condensation from shorting the sensor 401 9 Refer to Procedure 501 for the calibration instructions 400 1 Thermo Electron Laboratory Products Procedure 402 REPLACING CONTROL PROBE Single or Top Chamber of a Dual Chamber Unit 402 1 Turn off the power switch located at the top rear of the control housing Unplug the service cord from the electrical outlet 402 2 Remove two Philips head screws from ee as the sides of the control housing cover Lift straight up on the two rear corners to clear the access plates and pull cover rearward and up 402 3 Locate the control or water jacket sensor well in the left rear corner of the control housing visible through a hole in the left rear corner of the electrical deck Note that the opening of the well is sealed with silicone in order to prevent formation of condensation in the sensor well thus shorting the sensor This must be re sea
13. 2 Ground 3 Shield JPIO Power to 33686H02 Board Rh add on PCB 1 5 volts 2 n c 3 Ground 4 5 volts 5 Ground JP13 Door Switch Input 1 n c valve 2 Contact 3 Ground JP15 RS 485 w Power not used NOTE for future expansion JP16 RS 485 NOTE for future expansion JP18 CO Inject Valve Output 1 Valve Voltage 2 Switched Ground JP19 Auxiliary not used note for future expansion 600 2 JP5 Humidity Input from 33686H0X 1 Signal 0 1Vdc 2 Signal 3 n c JP7 Remote Alarm Contact Output 1 Common 2 NC with power OFF 3 NO with power OFF JP9 Temperature Signal Output 1 Signal 2 Ground JP11 not used JP12 not used JP14 Tank Switcher Inputs 1 Switched Ground to turn on 2 Valve Voltage 3 Ground 4 Tank 2 Sense 5 Ground 6 Tank 1 Sense JP17 Power to T C or IR Boards 1 n c 2 12 volts 3 n c 4 5 volts 5 Ground JP20 Low Water Indicator Input 1 Input ground reference 2 n c Thermo Electron Laboratory Products Display Board 31665Hxx The display board is very simple and the silkscreen shows all of its features JP1 is the header where the 16 wire conductor display cable is connected Pressing the Mute button causes the LED D1 next to the button to illuminate indicating that the audible alarm has been silenced The alarm will not sound until the Mute function is turned off by pressing the Mute butto
14. J12 return SET TEMP back to the desired operating temperature and install the control housing cover 8 Calibration is complete Allow unit to stabilize twenty four 24 hours before resetting SET OVERTEMP to the normal value 0 5c above the desired operating temperature 700 7 Thermo Electron Laboratory Products THERMAL CONDUCTIVITY CO2 CONTROL CALIBRATION PROCEDURE for Water Jacketed Models 300 301 3000 produced prior to March 93 and for all Forced Draft incubators produced prior to January 1 1998 l Remove control housing cover On the Main Control board unplug J4 to disconnect the CO2 solenoid and turn On DIP switch 1 to temporarily cause the TEMPERATURE display to indicate the raw CO2 board signal 2 Open and fan the glass door for two 2 minutes and leave the door open 3 With the glass door open press the CO2 Zero button on the front panel the CO2 Zero LED should light Close the door and observe that the Door Ajar LED continues to blink 4 Allow the temperature and humidity to stabilize 45 minutes before continuing with procedure 5 On the CO2 board adjust potentiometer VR2 Zero until the TEMPERATURE display indicates 0 0 adjust the Zero potentiometer on the front panel until the CO2 display indicates 0 0 Press the CO2 Zero touchpad on the front panel amp observe that the Zero LED turns Off 6A Connect the CO2 solenoid at J4 and set the Set CO2 to 5 ABOVE the desired opera
15. Looking for more information bh Artisan Visit us on the web at http www artisan scientific com for more information QUALITY INSTRUMENTATION GUARANTEED Price Quotations Drivers Technical Specifications Manuals and Documentation Artisan Scientific is Your Source for Quality New and Certified Used Pre owned Equipment e Tens of Thousands of In Stock Items Fast Shipping and Delivery Equipment Demos Hundreds of Manufacturers Supported Leasing Monthly Rentals Consignment Service Center Repairs InstraView Remote Inspection Experienced Engineers and Technicians on staff in our Remotely inspect equipment before purchasing with our State of the art Full Service In House Service Center Facility Innovative InstraView website at http www instraview com We buy used equipment We also offer credit for Buy Backs and Trade Ins Sell your excess underutilized and idle used equipment Contact one of our Customer Service Representatives today Talk to a live person 888 88 SOURCE 888 887 6872 Contact us by email sales artisan scientific com Visit our website http www artisan scientific com Thermo Electron Laboratory Products CELL CULTURE CO2 INCUBATOR Service Manual Full Featured Models November 2002 Thermo Electron Laboratory Products All rights reserved 97741H01 RevE Thermo Electron Laboratory Products 2001 Thermo Electron Laboratory Products All rights reserved No part
16. NTROL CALIBRATION PROCEDURE for Models 500 501 5000 produced prior to March 93 1 Remove control housing cover On the Main Control board unplug J4 to disconnect the CO2 solenoid and turn On DIP switch 1 to temporarily cause the TEMPERATURE display to indicate the raw CO2 board signal 2 Open and fan the glass door for two 2 minutes and leave the door open 3 With the glass door open press the Auto Zero touchpad on the front panel the Auto Zero LED should light and the CO2 display should extinguish 4 IMMEDIATELY close the glass door and observe that the Auto Zero LED extinguishes and the CO2 display illuminates This aborts the auto zero procedure after resetting the up and down counters on the CO2 board 5 On the CO2 board adjust potentiometer VR2 Zero until the TEMPERATURE display indicates 0 0 NOTE If unit will be used to maintain 15 CO2 or less use Step 6A If unit will be used to maintain 16 20 CO2 use Step 6B 6A Connect the CO2 solenoid at J4 set the Set CO2 to 5 ABOVE the desired operating CO2 setpoint and allow the unit to stabilize forty five 45 minutes Measure the CO2 content and compare to the CO2 setpoint Adjust the CO2 board potentiometer VR3 Span until the CO2 display matches the Measured content CAUTION if the Measured content is less than the Set CO2 value then disconnect the CO2 solenoid J4 before adjusting VR3 6B Connect the CO2 solenoid at J4 set the Set C
17. O2 to 20 and allow the unit to stabilize forty five 45 minutes Measure the CO2 content and compare to the CO2 setpoint Adjust the CO2 board Adjust the CO2 board potentiometer VR3 Span until the CO2 display matches the Measured content CAUTION if the Measured content is less than the Set CO2 value then disconnect the CO2 solenoid J4 before adjusting VR3 Repeat steps 2 5 until the unit holds 5 above the desired operating level of CO2 Repeat steps 2 4 Connect the CO2 solenoid set the Set CO2 to the desired operating level of CO2 and allow unit to stabilize forty five 45 minutes Measure the CO2 content and compare with SetCO2 If Measured is more than setpoint adjust potentiometer VR2 on the MAIN CONTROL board the largest PCB until the CO2 display matches the Measured content CAUTION if the Measured content is less than the Set CO2 value then disconnect the CO2 solenoid J4 before adjusting VR3 VO o N 10 Repeat Steps 8 and 9 one time 11 On the Main Control board turn off DIP switch 1 calibration is complete 700 9 Thermo Electron Laboratory Products Digital Infrared CO Sensor Retrofit Instructions Pre March 93 Version Single Chamber Only Installation Step 1 Disconnect incubator from power Step 2 Turn off CO gas supply to incubator Step 3 Remove control housing cover Step 4 Carefully disconnect the green plug power signal harness from the gas board as well as the gr
18. S WATR OFS Look at the CO2 Offset VIEW C OFS CO2 OFS Look at Humidity Offset VIEW H OFS RH OFS View Humidity Level VIEW RH VIEW RH 700 5 Thermo Electron Laboratory Products HI Display and Control Sensor Diagnostic RESISTANCE vs TEMPERATURE Degrees c Resistance Degrees C Resistance Normal Failure Mode Open infinite resistance 700 6 Thermo Electron Laboratory Products IV PRE MICROPROCESSOR BASED CONTROL PROCEDURES TEMPERATURE CALIBRATION for Water Jacketed Models 100 101 1000 300 301 3000 500 501 5000 for units produced prior to March 93 Read the entire procedure before commencing calibration 1 Install an NIST thermometer in the geometric center of the cabinet and adjust SET OVERTEMP to 55c allow the unit to stabilize temperature for twenty four 24 hours 2 Remove the cover from the control housing and locate the following parts on the large printed circuit board VR13 D68 J12 and J7 locate the Heat indicator on the front panel 3 Adjust the Temperature Display AND the Set Temp to match the thermometer 4 Unplug J7 and plug it in again D68 should now be lit 5 Install a jumper on J12 the Heat indicator on the front panel should now be either Off or On not blinking 6 Adjust VR13 until the Heat LED turns On then reverse direction to the point at which the Heat LED turns Off This is the desired position in which to leave VR13 7 Remove jumper from
19. ay interconnect cable from the sensor Step 5 Remove the tubular shield if provided from the sensor Step 6 Remove interior shelving and ductwork carefully lower the top air duct so as not to damage the gas sensor Step 7 Remove the sensor four thumb nuts internally from the fan gas port area Step 8 Remove the gas board four Phillips head screws from the electrical deck Step 9 Detector removal now complete Step 10 Installation of Digital IR detector assembly Step 11 Carefully unwrap new IR sensor and board Step 12 Carefully disconnect ribbon cable from sensor Step 13 Install new sensor in the same sensor location using the existing gasket and four thumbscrews Use caution during installation so as not to damage the circuit board on top of sensor Step 14 Install adapter plate assembly in the same position of the previously removed gas board using the same hardware and kit provided flat washers Step 15 Install new gas board onto the adapter plate using the screws and washers provided DO NOT use the flat washers These should have been used performing step 2 0 4 Orient the new board so that the black screw type connector is on the right as you face the front of the incubator Step 16 Loosen the screws in the green connector previously disconnected from the old gas board and remove the four wires 700 10 Thermo Electron Laboratory Products Step 17 Install the 18 gauge black wire under the screw in the bla
20. ay matches the analyzer b Open glass door 45 degrees and close immediately repeat steps I through Illa one time IV Setpoint Adjustments a Allow system to stabilize at 15 CO2 and measure with analyzer Press release the CALIBRATE key until the alphanumeric display indicates CALIB CO2 Press the UP or DOWN keys until the CO2 display matches the analyzer b Allow system to stabilize at 10 CO2 and measure with analyzer Press release the CALIBRATE key until the alphanumeric display indicates CALIB CO2 Press the UP or DOWN keys until the CO2 display matches the analyzer c Allow system to stabilize at 5 CO2 and measure with analyzer Press release the CALIBRATE key until the alphanumeric display indicates CALIB CO2 Press the UP or DOWN keys until the CO2 display matches the analyzer d The display will return to normal operation automatically approximately ten 10 seconds after completing the offset 700 12 Thermo Electron Laboratory Products Addendum for CO offset adjustment on IR units If one wishes to use the IR sensor based unit within 0 5 of 2 5 and 7 5 the offsets must be adjusted at 1 5 and at 5 Accomplish this by setting the CO at 5 0 and checking the CO level with a Fyrite CO calibration device then introducing the appropriate offset using the CALIBRATE key View the CO offset at 5 0 by pressing the Set CO key and the SET TEMP key simultaneously and make a note of this value Then
21. be set lower than TEMP SETPOINT 0 1 C Simultaneously press the OVERTEMP key and the increment or decrement key The CO2 STATUS Alphanumeric display will show SET OV TEM while you are changing the value but will soom mas casero sure automatically go blank within 10 seconds of the last change CO Setpoint 0 to 20 Simultaneously press the SET CO2 key and either the increment or decrement key This setting and all subsequent ones give similar displays Door Heater Duty Cycle Door heat is controlled by the microprocessor and is not user adjustable CAUTION Before the incubator temperature and CO levels have stabilized there will be error messages in the alphanumeric display that indicate warnings Allow the incubator to stabilize a minimum of eight 8 hours before performing any calibration procedures 300 1 Thermo Electron Laboratory Products View Setpoints Operating Temperature Press SET TEMP key The digital temperature display will read the temperature setting The alphanumeric display will show the SET TEMP message and the CO3 STATUS alphanumeric display will go blank after approximately 10 seconds Overtemperature Setpoint Press the SET OVERTEMP ke
22. ber Place this label directly under the serial number label This label is located inside the outer door on the lower right side of the cabinet Stabilize Incubator Close glass door and allow incubator to stabilize for 24 hours before customer use Note Water may be added to the humidity pan after this stabilization period 237 NOTE After the incubator is stabilized if the temperature display disagrees with the user s preferred standard it may be necessary to perform an additional temperature offset 700 16 Thermo Electron Laboratory Products VII Sliding Out the Control Housing for the Bottom of a Double Chamber Incubator Step 1 Unplug the Service Cord Step 2 Remove the Lower chamber front panel assembly and disconnect the display board ribbon cable Step 3 Remove the two L brackets two screws each at each end of the control housing Step 4 Remove the front panel retainer 2 screws spanning the top edge of the control housing opening Step 5 On the TOP incubator a Remove the top cover b Disconnect the AC cable going to the terminal strip on the wiring deck c Ifa tank switcher is installed disconnect the AMP connector at JP14 VALVE SYS This is found at the upper left corner of the Control Board d Take the AC cable and the tank switcher wires and feed them down into the wire chase as far as possible The connectors should not fall into the chase Step 6 If a tank switcher is installed cut t
23. binet Control Microprocessor C02 15 PSIG supplied to fitting s on back of unit Single Chamber Cabinet Water 11 5 gallons 43 5 Liters of Tap or distilled water DO NOT USE DE IONIZED WATER Remote Alarm A terminal strip on the rear of the cabinet contains three 3 screws These terminals are connected to Common Normally Open and Normally Closed dry relay contacts The Remote Alarm terminals are switched immediately on power up When an alarm condition occurs the alarm contacts will be switched to the de energized state The remote alarm contacts indicate a power failure because the alarm relay is de energized when AC power is removed Circuit Breaker A button labeled 5 will reset the circuit breaker if for any reason it trips Main Power Switch Power switch located on rear of control housing Always use this switch to turn power off when any work is done on the unit 75 Watt Outlet This is a convenience outlet for the operator s use Do not exceed 0 6A load on this outlet CO Inlet Fittings There are two fittings Two barbed fittings are installed in these openings when the optional tank Double Chamber Cabinet switchover has been ordered otherwise is only one fitting is installed Access Port This port is used for instrumentation of any kind 200 1 Thermo Electron Laboratory Products Optional RS232 Port This optional 9 pin connector is for PC computer access to the built in data logging ca
24. bserve the CALIB CO2 message and that the CO2 display begins to blink While the display is blinking press the UP or DOWN key until the display matches the CO2 content measured in Step 508 1 above Reconnect the CO2 supply the system will automatically exit the Calibration mode if no keys are depressed for approximately fifteen seconds The system will now equilibrate based on the new value 500 7 Thermo Electron Laboratory Products Section 600 PCB Description amp Pin Outs e Main Control Board 31664Gxx Following is a table listing the LEDs and their messages D101 On if the 12V dc supply is functioning properly D15 D13 D14 D12 D11 D10 D9 On when the micro is calling for the Main heater to energize Off otherwise On when the micro is calling for the Fan to turn Off otherwise On when the micro is calling for the Door heater to energize Off otherwise On when the micro is calling for the CO inject valve to energize Off otherwise On when the micro is calling for the Tank Switching Solenoid to energize Off otherwise Blinking On and Off to indicate micro is executing program properly On when the water probe is used as the temperature reference for controlling the chamber heater D8 is not used Off DIP Switch Functions DIP switch 1 is on for customer use and the off position enables diagnostic information to be sent to remote terminal at 9600 bps DIP switch 2 no
25. characters In the past on one or two occasions it has been necessary to use an 8 character display necessitating a slight change in the message displayed An 8 character display can be easily changed by merely unplugging it and plugging in a 10 character display DIP switch 8 will then need to be turned Off Message Meaning 10 Character 8 Character Audible Display Display Alarm Water is Low ADD WATER ADD WATER Yes CO2 Tank No 1 is Empty TANK 1 OUT TK 1 OUT Yes CO2 Tank No 2 is Empty TANK 2 OUT TK 2 OUT Yes Call for Service SYSTEM ERR SYS ERR Temp Display Calibration Mode CALIB TEMP CAL TEMP CO2 Calibration Mode CALIB CO2 CAL CO2 Temp Control Calibration Mode CALIB WATER CAL WATR RH Calibration Mode CALIB RH CAL RH Automatic CO2 Zero Mode AUTO ZERO AUTOZERO Excess CO2 Alarm HIGH CO2 HIGH CO2 Yes Deficient CO2 Alarm LOW CO2 LOW CO2 Yes Overtemperature Warning HIGH TEMP HI TEMP Yes Undertemperature Warning LOW TEMP LOW TEMP Glass Door is Open DOOR OPEN DR OPEN Display Probe has Failed PROBE ERR PRB1 ERR Temp Control Probe has Failed PROBE2 ERR PRB2 ERR CO2 Sensor has Failed CO2 SENSOR CO2 SNSR Action to Start Auto Zero CLOSE DOOR SHUT DR Auto Zero was Aborted ZERO ERROR ZERO ERR Set Operating Temp SET TEMP SET TEMP Set Overtemp Alarm Threshold SET OVTEM SET OV T Look at the Temperature Offset VIEW T OFS TEMP OFS Look at the Water Temp Offset VIEW W OF
26. ck connector on the new gas board labeled V Install the 18 gauge Red w White wire under the screw labeled V Install the 22 gauge black wire under the screw labeled COM and finally install the 22 gauge yellow wire under the screw labeled Vo Step 18 Connect the ribbon cable from the new gas board to the newly installed sensor Step 19 Remove mylar overlay from control panel as necessary and cut a 1 8 diameter hole for access to the potentiometer next to the Auto Zero key Replace overlay Step 20 Perform the CO2 Calibration procedure found on Page 35 before using unit 700 11 Thermo Electron Laboratory Products V Infra Red CO2 Calibration Procedure for 500 5000 Series Water jacketed or Radiant Wall units produced March 93 August 1996 I Zero Adjustment Fan the glass door for ninety seconds and then allow temp to equilibrate With 0 CO2 in the chamber adjust CO2 board pot ZERO until 0 00V DC 0 003VDC is measured between pins 3 and 4 on the 5 pin Power CO2 connector II Auto Zero a Open the glass door and press the CALIBRATE key and the CO2 display will extinguish leave the door open b After several minutes the alpha numeric display will instruct you to CLOSE DOOR close the door and the CO2 display will indicate 0 00 CO2 III Gain Span Adjustment a With chamber stabilized at a setpoint of 20 CO2 display indicates 20 for 10 15 minutes adjust CO2 board Span pot until CO2 displ
27. cket on each side Remove the brackets and their Phillips head screws retain for use later 409 2 2 6 Grasp the PCB tray and pull it straight out to the limit of the harness 409 2 2 7 From the Upper chamber push more wire into the wiring channel 409 2 2 8 Repeat steps 409 2 2 6 and 409 2 2 7 until sufficient clearance is gained to replace the desired component Pre 1999 Rh sensors are mounted from above the ceiling and require the PCB tray to be completely removed to access 1999 present Rh sensors are mounted from within the chamber and access only to the respective Rh PCB is required 409 2 2 Single Chamber or Either Chamber of a Dual Chamber unit 1999 Present 409 2 2 1 Remove the top air duct over the CO sensor and access port by removing all shelves and one side duct sheet inside the chamber to reveal the four screws or nuts 409 2 2 2 Remove the four screws separate the wiring harness at the Molex connector and remove the sensor assembly 409 3 Label and disconnect all electrical connections on the sensor and electrically attached PCB s see diagram next page 409 4 Remove the PCB mounting screws and replace the IR Rh PCB or the TC CO2 PCB 400 7 Thermo Electron Laboratory Products 409 5 409 6 409 7 409 8 Replace the sensor and printed circuit board if applicable and follow above steps 409 1 409 4 in the reverse order With a temperature setpoint of 37 C and no water in the c
28. continuous power to heater 501 10 2 On Power Board connector JP4 verify 115 Vac to the chamber heater between pins 2 amp 3 501 10 3 On Power Board connector J1 verify 115Vac to the door heater between pins 1 amp 2 501 10 4 Return SET TEMP to the customer s desired temperature 500 2 Thermo Electron Laboratory Products Procedure 502 Chamber Display Temperature Offset The chamber temperature probe should read the correct temperature within 0 1 C If this is not the case you will remedy the problem by introducing a temperature offset at the display panel as follows 502 1 502 2 502 3 502 4 502 5 Check the probe by placing a thermocouple or an NIST calibrated glass stem thermometer in the chamber allowing the chamber temperature to stabilize If the reading on the thermometer is different from the Temperature display on the control panel press the CALIBRATE key once Observe the CALIB TEMP message and that the temperature display begins to blink While the display is blinking press the UP or DOWN key until the display matches the temperature measured in 502 1 above System will automatically exit the Calibration mode if no keys are depressed for approximately fifteen seconds The system will now equilibrate based on the new value Remove thermocouple used in 502 1 and resume normal operation 500 3 Thermo Electron Laboratory Products Procedure 503 Water Jacket Control Temperature Offset
29. crews separate the wiring harness at the Molex connector that is tucked under the CO Power Board and remove the CO2 sensor assembly 404 4 Install new sensor in the reverse order 404 5 Plug in unit and turn on the main power switch 404 6 Allow temperature to stabilize and calibrate the CO2 control see Procedure 505 or 506 Remote Alarm Terminal Microprocessor Control Power PCB Rh PCB IR Only Rh Section CO2 Outer Door 400 9 Thermo Electron Laboratory Products Section 500 ADJUSTMENT PROCEDURES Calibration of the incubator is done using either control panel readout offsets or adjustment to the electronic circuit boards themselves Offsets are possible only within pre established limits Both methods are outlined below Circuit board calibrations must be performed any time circuit boards or sensors are replaced NOTE Make sure all sensors are plugged in and that all connectors are fully seated has been operating with one constant SET TEMP for a minimum of eight 8 hours before CAUTION Calibrations can only be performed on a thermally stable unit Ensure that the cabinet attempting any calibrations e Procedure 501 Basic System Electrical 501 1 Verify DIP switch settings conform to the following pattern 1 ON always 2 OFF always 3 OFF always 4 ON for top chamber of a Double only 5 OFF always 6 ON when Rh option is present 7 ON when Tank Switcher option present
30. d circuit board and follow above steps 1 4 in the reverse order GE NOTE You must calibrate the system whenever you replace a circuit board or sensor Refer to applicable calibration procedure s 400 6 Thermo Electron Laboratory Products Procedure 409 Humidity Sensor DANGER Printed circuit boards and the Humidity sensor are Electrostatic sensitive all standard ElectroStatic Discharge precautions MUST be observe to prevent damage to components 409 1 Turn off the main power switch located at the top rear of the control housing and unplug the service cord from the wall outlet 409 2 Access the component to be replaced 409 2 1 Single Chamber unit or Top Chamber of a Dual Chamber unit Pre 1999 409 2 1 1 Remove the incubator top cover and locate the humidity sensor amp PCB 409 2 1 1 Lower Chamber of a Dual Chamber unit 409 2 2 1 Remove the top cover from the control housing 409 2 2 2 Label and disconnect the cables that exit the control housing and are routed through the wiring channel on the back of the upper chamber Push slack into the wiring channel 409 2 2 3 Open the outer door of the lower chamber and remove the single Phillips head screw from the left and right sides underneath the display panel 409 2 2 4 Grasp the display panel and pull it forward swing the lower edge of the display panel away from the cabinet and lift the panel off retaining bar 409 2 2 5 The PCB tray is retained by an L shaped bra
31. e condensation next day 503 7 1 If condensation exists on the glass door increase door heat by lowering the water jacket temperature repeat steps 503 2 amp 503 3 then press the Up arrow key until the Temperature display indicates a value of 0 1 0 2 C higher 503 7 2 If condensation exists on the cabinet floor decrease the door heat by increasing the water jacket temperature perform steps 503 2 amp 503 3 then press the Down arrow key until the temperature display indicates a value of 0 1 0 2 C lower 503 8 Repeat step 503 7 if required 500 4 Thermo Electron Laboratory Products Procedure 504 Humidity Sensor Offset Adjustment optional equipment gt 504 1 504 2 504 3 504 4 NOTE Although a circuit board Zero adjustment was discussed in Procedure 501 8 above an offset can be introduced to match any reliable instrument as follows Fill the humidity pan with tap or distilled water NOT deionized water place it inside chamber and allow to stabilize overnight with the Humidity measurement probe inside Without opening the glass door measure the humidity level inside Press and release the CALIBRATE key three times observe that the Rh display blinks and the CALIB RH message 1s seen on the alphanumeric display Press the UP or DOWN key until the display agrees with the test instrument value After 10 seconds the display will return to normal Remove the humidity measurement probe Procedure 505 Th
32. e eee cece eee eee ne ene ee ek ee pg 600 1 Display Board 31665H01 or 31665HO02 esse ee ese ese ee cece eee ke es pg 600 3 Humidity Amplifier IR systems only esse ese ese ee ese ee ee ee se ee ke ee pg 600 3 Power Board 3IOOGHOL sasie se Es SE NESER EES GEE RED ER GED SERS einas pg 600 4 THC Board 33686H01 Description and Pin Outs 00 pg 600 5 Section 700 APPO 0 RE a EE GE GR GE DES pg 700 1 100 1 Thermo Electron Laboratory Products Section 100 PRODUCT OVERVIEW Interior tanks are surgical 304 stainless steel All interior parts are easily removable for cleaning and decontamination Cabinet insulation is foamed in place polyurethane and contains no CFC s These incubators advise the operator on the status of the unit using an alphanumeric display controls self diagnosis functions and alphanumeric displays are accomplished by a microprocessor based controller The various touchpads on the control panel have multiple functions and can be operated in combinations Controls self diagnosis functions and numeric displays are accomplished by a microprocessor based controller When power is initially applied to the unit the system will indicate HELLO while the unit performs a self test Upon completion of the test the system will assume normal operation with the current Temperature and CO2 values displayed Other parameters can be viewed from the digital display by a use of single or a set
33. ermal Conductivity CO2 Printed Circuit Board Calibration 505 1 505 2 505 3 505 4 505 5 505 5 505 6 Set CO2 Setpoint to 0 0 open and fan the glass door 30 seconds to remove CO2 from the chamber Allow temperature to stabilize to within 0 2c of setpoint approximately 30 45 minutes and use Fyrite to verify 0 0 CO2 inside the chamber If JS exists adjust R23 Zero pot to achieve 0 00VDC with the meter probes connected across the two pins of the CO2 PCB connector J101 The acceptable range is 0 003 to 0 003 volts If J5 does not exist adjust R23 Zero pot to achieve 0 3000VDC with the meter probes connected across the two pins of the CO2 PCB connector J101 The acceptable range is 0 303 0 297Vdc With the meter connected across the two pins of CO2 PCB connector J5 adjust R27 Span for a reading of 0 297V to 0 301V dc If the CO2 display value is gt 0 2 then perform an AutoZero calibration Procedure 507 before continuing Adjust SET CO2 to 10 and allow the unit to stabilize 10 additional minutes after the chamber reaches setpoint before measuring Disconnect or pinch off the CO2 supply Using a CO2 analyzer measure the CO2 content and adjust R24 Gain until the CO display matches the analyzer reading Reconnect the CO2 supply Procedure 506 Infra Red CO2 Calibration 506 1 506 2 506 3 eS eS 506 4 506 5 Set CO2 Setpoint to 0 0 open and fan the glass door 30 seconds to remove CO2 from the chamber
34. ey four times The alphanumeric display will read 31 A a K CALIE ER CALIB RH and the CO Display will have the RH level blinking in its window SET ERTEMP SET CO2 RH 300 5 Thermo Electron Laboratory Products Section 400 COMPONENT REPLACEMENT Procedure 401 REPLACING DISPLAY PROBE 401 1 Turn off the power switch located at the top rear of the control housing Unplug the service cord from the electrical outlet 401 2 Open the glass door and remove the ceiling plenum 401 2 1 Remove all shelves and the humidity pan if it exists 401 2 2 Grasp the bottom of the left or right false wall and pull toward the center of the chamber to free the shelf support assembly 401 2 3 Disassemble and remove the support assembly and Plastic the ceiling plenum 401 3 Locate the temperature display sensor and remove the thumbnut securing the harness plastic anchor to the access plate 2 NOTE DO NOT discard the plastic anchor 401 4 Grasp the sensor harness and pull firmly to break the silicone seal and free it from the stainless steel sheath 33 NOTE DO NOT discard the metal sheath 401 5 Remove the four thumbnuts holding the access plate in place and remove the sensor harness from the access plate 401 6 Remove two Philips head screws from the sides of the control housing cover Lift straight up on
35. from the Main Control PCB connector JP8 and withdraw it from the cabinet ACCESS TO LOWER CHAMBER PROBE NOTE When replacing a probe in the Lower chamber of a dual chamber model withdrawal and replacement of the sensor will require removing the wiring chase from the rear exterior of the chamber in order to access the sensor harness 403 5 Install the new sensor in the reverse order sealing the opening of the probe well with silicone to prevent condensation from shorting the sensor 403 6 Refer to Procedure 503 for the calibration instructions 400 3 Thermo Electron Laboratory Products Procedure 404 REPLACING CHAMBER FAN The chamber Fan is held in place by four Phillips head machine screws with lock washers accessible from inside the incubator chamber 404 1 Turn off the main power switch located at the top rear of the chamber Unplug the service cord from the wall ae outlet Assembly Gasket 404 2 Remove the top air duct over the CO sensor and access port by removing all shelves and one side duct sheet inside the chamber to reveal the four screws 404 3 Remove the four screws separate the wiring harness at the Molex connector that is tucked under the CO Power Board and remove the motor and fan assembly from above 404 4 To replace follow the reverse procedure 404 5 Plug in unit and turn on the main power switch Procedure 405 REPLACING FUSES There are five fuses on the CO Power B
36. hamber allow to stabilize overnight with a psychrometer inside the chamber The next day press hold SET TEMP and VIEW RH and use the front panel UP or DOWN button to bring the existing Rh Offset to 0 0 if necessary Adjust R4 on the humidity PCB IR daughter board mounted on the main control PCB TC humidity section of the CO2 PCB Press the CALIBRATE button three times and use the front panel UP or DOWN button to bring the Humidity display to match the measured value With temperature stabilized place water in the humidity pan and allow to stabilize overnight with a psychrometer inside the chamber After stabilization press the CALIBRATE button three times and adjust the displayed Rh value to match the measured value Remote Alarm Terminal Strip Microprocessor Control PCB Power PCB Rh Section TC Only CO2 PCB Outer Door Control Housing PCB layout 400 8 Thermo Electron Laboratory Products Procedure 409 CO2 Sensor DANGER Printed circuit boards and the Humidity sensor are Electrostatic sensitive all standard ElectroStatic Discharge precautions MUST be observe to prevent damage to components 404 1 Turn off the main power switch located at the top rear of the chamber Unplug the service cord from the wall outlet 404 2 Remove the top air duct over the CO sensor and access port by removing all shelves and one side duct sheet inside the chamber to reveal the four screws 404 3 Remove the four s
37. hanging its value but reverts to steady 10 seconds after you have released all keys NOTE If you press the CALIBRATE key unintentionally merely close the door or if it is D closed open it immediately In either case the ZERO ERROR will show in the alphanumeric display Press the CALIBRATE key once and all displays are restored to normal e Temperature Display Offset If you wish to place a temperature display offset into the control panel display to match any reliable thermometer such as the Fluke thermocouple do the following Step 1 Press the CALIBRATE key E once The alphanumeric display J mn ACTIETERR will show CALIB TEMP and the temperature display will begin blinking SET OVERTEMP SETCO2 RH Step 2 Press either the increment key or decrement key to change the temperature display Step 3 When you have finished setting the offset the display will stop blinking after 10 seconds 300 4 Thermo Electron Laboratory Products e Humidity Offset option Factory calibration of Relative Humidity RH is done using instruments with accuracy of 1 0 or better If you have an instrument similar to this and you wish to place an offset into the displayed RH reading Press the CALIBRATE key three times Unless you have initiated the water calibration mode Then you must press the CALIBRATE k
38. he operating coil electrical leads are very small gauge 406 3 Unplug the solenoid from the Main Control PCB connector JP18 remove the plastic tubing from the barbed fittings on the valve and remove the valve mounting screws from the valve bracket Be careful to retain the rubber washers installed on the mounting screws 406 4 Install the replacement solenoid in the reverse order e Procedure 407 Replacing Pressure Switches The pressure switches PS1 and PS2 in the tank switcher assembly have push on terminals which make the changing of these switches very simple 407 1 Turn off the main power switch located at the top rear of the chamber Unplug the service cord from the wall outlet 407 2 Remove the lower chamber display panel pull out the electrical deck see Appendix and locate the switch to be replaced 407 3 Remove the switch mounting screws disconnect plastic CO gas tubing and electrical leads 407 4 Remove old switch and install new one by following steps 1 3 above in reverse order 400 5 Thermo Electron Laboratory Products Procedure 408 Printed Circuit Boards DANGER Printed circuit boards are Electrostatic sensitive all standard ElectroStatic Discharge precautions MUST be observe to prevent damage to the printed circuit boards 408 1 Turn off the main power switch located at the top rear of the control housing and unplug the service cord from the wall outlet 408 2 Access the compone
39. he tyraps securing a CLEAR HOSE near the Bottom chamber sensor port clearance hole Step 7 Slide out the bottom control housing by holding the wiring deck on each corner and pull it out the front pulling it out only as far as necessary to provide service EF NOTE The deck is designed to pull out far enough to reach the screws at the front of the Control and Power boards If you meet resistance in your effort to pull out the deck a Be sure that the wires at the TOP incubator are fed into the wire chase as far as possible without forcing the connectors into the chase b Place your hand under the wiring deck and lift up slightly as you pull it toward you c Be sure you removed the front panel retainer Step 8 Sliding the control housing back in involves a process of pulling the AC Cable and Tank Switcher wires at the TOP incubator back up through the wire chase as you push the control housing in This is accomplished by pushing the deck in a few inches at a time and then pulling the wires up the wire chase DO NOT attempt to push in the deck without pulling these wires up You can not expect the wires to defeat gravity and flow up the wire chase on their own Push the housing in far enough to replace the two L brackets Step 9 Route the Clear Hose around the sensor port clearance hole such that there is no risk of it contacting the fan and then tyrap it LOOSELY to the tyrap anchors Step 10 At the TOP incubator replace the AC cable and Ta
40. he water temperature Calibrate Water Temperature Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Go to calibrate mode by simultaneously pressing the SET TEMP SET OVERTEMP and SET CO2 buttons An additional decimal will appear in display Push CALIBRATE button one time EE until display reads CALIB TEMP 31 4 n N Adjust with UP or DOWN key until display matches thermometer srme lose orcos me wy A Y measurement from Step 1 Push CALIBRATE button additional times until display reads CALIB WATER Record thermocouple and water display temperatures Wait for displays to stop flashing approximately 10 seconds Push calibrate button three 3 times until display reads CALIB WATER Using the increment and decrement keys on the control panel adjust flashing temperature displayed value equal to 0 5 c hotter than the water temperature recorded in Step 4 above Calibrate Air Temperature Step 12 Step 13 Wait for displays to stop flashing approximately 10 seconds a q mn Push calibrate button one time until d At LH display reads CALIB TEMP TE STONE IKT n Al Y 700 14 Thermo Electron Laboratory Products Step 14 Using the increment and decreme
41. into place Step 2 Re install top and front on double unit Control panel housing cover 700 15 Thermo Electron Laboratory Products Remove Gas Inject Plate Step 1 Remove gas inject plate by removing four thumbnuts Note tubing connections Step 2 Carefully pull and remove sensor from protective sheath and through hole in sensor plate Clean off any residual silicon RTV adhesive Install New Gas Inject Plate Step 1 Carefully slide sensor through 1 4 hole located between two tubing connector fittings in new gas inject plate Step 2 Slip small white snap bushing on sensor and up its length until it fits snugly in hole Step 3 Re install tubing to proper connectors on new gas inject plate Step 4 Re install new gas inject plate in top of chamber Do not re install thumbnut in corner opposing gas inject tube Step 5 Install probe sheath tube clamp with last thumbnut Step 6 Slide sheath in clamp Fill protective sheath with silicon RTV sealant Fully insert sensor into sheath and seal end with additional silicon Install New Humidity Pan Ultima seamless incubators only replace bottom support and humidity pan with new set in kit Elite welded incubators only install humidity pan support channels in lower most shelf support slots and pan per humidity pan kit installation instructions Add Deviation Label Enclosed in the upgrade kit is a small aluminum label showing the upgrade deviation num
42. ired in HO1 version T C Board 28860H03 Pin Outs J2 T C CO Sensor Input J3 Power Input 1 Shield 1 n c 2 G 2 12 volts 3 G 3 n c 4 B 4 5 volts 5 Shield J101 CO2 Output to control board 1 Signal 2 Ground The signal range on the output of the TC board for a 0 20 CO range is OVdc 1Vdc The zero should be set at 0 300 Vdc when no CO is present at the sensor At 10 the span should be adjusted to read 0 8 VDC Refer to calibration instructions 50 mVdc 1 0 CO Please note temperature must remain stable within 0 20 C of setpoint during span adjustment 600 5 mm N Thermo Electron Laboratory Products Section 700 APPENDIX RS 232 Communications Ge ee ee ee Alphanumeric Plain Language Messages Temperature Sensor Resistance vs Temperature Chart sesse esse ese esse see Pre Micro Procedures Temperature Calibration sis esse GEES ES SEE REG GES GE DE EG GR RE GE AG GE eg Thermal Conductivity TC CO2 Control Infrared IR CO2 Control 0 0 ses cece eee es Digital Infrared CO2 Sensor Retrofit Instructions IR Calibration Procedure March 93 August 1996 1994 Temperature Upgrade Field Installation Instructions 606 Sliding Out the Lower Chamber Electrical Deck Reference Tables cccccceveccoevececeveceses Wiring Diagram Water Jacketed and Radiant Wall
43. is CO setpoint Field 5 is temperature reading Field 6 is temperature setpoint Field 7 is high temperature alarm setpoint Field 8 is humidity reading if option is present Field 9 is record count from 0 to 4000 700 3 Thermo Electron Laboratory Products The counter cannot be reset it automatically resets when it reaches 4000 The screen also provides information that duplicates functions on the user interface panel The logged parameters are displayed on the screen except the record count If the incubator is not responding to the computer the message COMMUNICATION ERROR is displayed Check the link between the computer and the incubator Make sure that the port in use is dedicated The mouse can not share the same serial COM port with the data this acquisition software An IR or TC on the top right hand side of the computer monitor just below the time clock will indicate whether the incubator is a TC CO2 sensor thermal conductivity or IR infrared CO sensor based incubator e Fault and diagnosis Fault The message Communication Error is displayed on the screen Diagnosis Check the cable between the computer and the incubator Verify continuity of the wires from point to point Make sure that the cable satisfies the connections requirements stated in sub heading Requirements 700 4 Thermo Electron Laboratory Products II Alphanumeric Display Messages The standard array for the alphanumeric display is 10
44. key pad combinations these will be discussed in detail later CO gas control is achieved by the infrared or thermal conductivity method The infrared sensor measures the transmission of a specific wavelength of light that is proportional to the CO2 concentration The thermal conductivity sensor measures the amount of heat conducted away from a thermistor due to a change in electrical resistance which is proportional to the amount of CO2 within the chamber A circular High Efficiency Particulate Accumulator HEPA filter is fitted to the inlet of the circulation fan opening to continuously scrub airborne particulates to improve air quality and protect cultures from contamination and results in a complete air exchange and filtering process every two minutes The filter is installed in the ceiling plenum by inserting the mounting tabs into the plenum at the fan opening and giving the filter a one eighth turn Removal of the filter is performed in the reverse procedure The filter should be changed every 2 3 months or if deterioration of CO2 control is observed 100 2 Thermo Electron Laboratory Products Section 200 CONSTRUCTION Model 300 301 3000T 7 500 501 5000T 7 Single chamber top controls 300 301 3000D 7 500 501 5000D 7 Dual chamber top controls Tank 304 surgical stainless steel Holds 11 5 gallons Insulation Structural foam insulation Drain Quick Disconnect drain hose fitting located at bottom left side front corner of ca
45. led when the replacement procedure is complete 402 4 Unplug the sensor from the Main Control PCB connector JP8 and withdraw it from the cabinet CONTROL PROBE 402 5 Install the new sensor in the reverse order sealing the opening of the stainless steel sheath with silicone to prevent condensation from shorting the sensor 402 6 Refer to Procedure 502 for the calibration instructions 400 2 Thermo Electron Laboratory Products Procedure 403 REPLACING CONTROL PROBE Lower Chamber of a Dual 403 1 Turn off the power switch located at the top rear of the chamber Unplug the service cord from the electrical outlet 403 2 Remove two Philips head screws from the sides of the control housing cover Lift straight up on the two rear corners to clear the access plates and pull cover rearward and up CONTROL HOUSING 403 3 Locate and remove the access panel at the rear of the left panel located between the Upper and Lower chambers and find the black sensor harness 403 4 Locate the control or water jacket sensor well in the left rear corner of the water jacket visible through a hole in the left rear corner of the mid section Note that the opening of the well is sealed with silicone in order to prevent formation of condensation in the sensor well thus shorting the sensor This must be re sealed when the replacement procedure is complete UPPER CHAMBER CONTROL PROBE 403 4 Unplug the sensor
46. lphanumeric display This aborts the auto zero process and you must press CALIBRATE once to clear the error and begin over CAUTION If the door is closed before this message is displayed or if you close the door and Step 4 After you have closed the door no further action is required The CO z oe sers display will remain blanked out 31 while the auto zero process is z running This is five minutes for the ENE A Ee units with an infrared CO sensor In units with thermal conductivity TC sensors the period will be at least five minutes plus the time required for the cabinet to attain setpoint temperature minus 0 2 C 300 3 Thermo Electron Laboratory Products Method 2 Display Offset Step 1 Run a test for CO level using an instrument such as a Fyrite tester to see if the result agrees with tile CO display The factory uses traceable gas standards yielding an accuracy of 0 2 for calibration Step 2 Press the CALIBRATE key twice The CO display will blink and j n Ta 7 ae the alphanumeric display will read L LLL CALIB CO2 me oe sesse es ae Step 3 Press either the increment or decrement key to change the display to match your reading The display blinks while you are c
47. n again The 10 character alphanumeric display will show the status of the incubator when keys are pressed or when an alarm condition exists otherwise the display is blanked Indicator LED D1 red Mute LED lights next to the mute button on the display panel to show that the audible alarm has been silenced JP1 Control Board Data Cable 1 12Vdc 3 5Vdc 9 Chassis amp Signal Ground Humidity Amp Board 33686H02 Pin Outs J1 Humidity Sensor Input 2 12Vdc 4 8 Serial Communication Data 10 Chassis amp Signal Ground J2 Power Input from control board 1 5 volts J4 Humidity Output to control board 2 Ground 1 Signal 3 5 volts 2 Ground 4 Ground J5 Not Used note J5 can be used to measure humidity output 1 gt 2 The humidity output at J4 should match the display reading 100 The humidity output voltage range should be 0 to 1 0 Vdc The resolution of the humidity display is 0 2 The maximum humidity value that will be displayed is 98 4 600 3 Thermo Electron Laboratory Products Power Board 31666H01 The power board drives the door heater main heater fan heater and strip heater The strip heater is on 100 of the time The fan is turned off when the door is opened and turned on when the door is closed The door heater is turned on and off as determined necessary by the microprocessor to prevent formation of condensation on the glass door the higher the duty cycle
48. nk Switcher connector Replace the top cover Continued 700 17 Thermo Electron Laboratory Products Step 11 At the BOTTOM incubator replace the L brackets and the Front Panel Retainer Connect the display board and reinstall the front panel NOTES Regarding the Double Incubator 1 If you need power to the BOTTOM incubator while the deck control housing is pulled out be sure that the power switch is ON before pulling the deck out as it will be impossible to reach it After you have the deck where you want it plug in the service cord Ensure that the Upper incubator AC cable is not shorting against anything before applying power to the Bottom chamber 2 The BOTTOM incubator CAN be pulled out just enough to reach the IR or the T C board for calibration purposes while the TOP incubator remains undisturbed If this is desired pull out the BOTTOM deck just enough to reach the potentiometers in need of adjustment The TOP incubator CAN NOT remain powered up while the BOTTOM deck is pulled out beyond this point i e the AC cable at the TOP incubator must be disconnected 700 18 Thermo Electron Laboratory Products VIII REFERENCE TABLES Table 1 Typical CO2 Sensor Signal Values 37 C Measured on the CO2 PCB Measured Value Thermal Conductivity TC Infra red IR Between Connector J5 Pins 1 amp 2 Vo and COMM 0 0 mV 0 mV 5 15 mV 250 mV 10 35 mV 500 mV 15 50 mV 750 mV
49. nt keys on the control panel adjust flashing temperature displayed value equal to previously recorded center air thermocouple temperature or users preferred standard Set Control Temperature Offset Step 15 Push calibrate button three 3 times until display reads CALIB WATER Step 16 Using the increment and decrement keys on the control panel adjust flashing water temperature display until it indicates a value of 9 C above chamber temperature display Disconnect Power Turn off incubator power and disconnect power cord Replace EPROM Remove control housing cover If double unit remove front control panel of bottom unit Double chamber only Disconnect ribbon cable from printed circuit board Remove chassis retaining clips from left and right of control housing Carefully slide chassis out until you meet resistance Feed wire harness and tubing from top chamber into rear wire chase Pull chassis out further until EPROM is accessible If double unit repeat process for lower chamber ao Tp Single and Double chambers a Replace EPROM with version 2 0 EPROM using EPROM puller Note orientation of notch on EPROM b Orient new EPROM in same position Disconnect Strip Heater Disconnect top strip heater from main harness by disconnecting both black wires Perimeter heater black and white wired connector MUST remain connected Re install Control Chassis Step 1 Re connect ribbon cable until it snaps
50. nt to be replaced 408 2 1 Single Chamber unit or Top Chamber of a Dual Chamber unit 408 2 1 1 Remove the incubator top cover and locate the circuit board to be replaced 408 2 2 Lower Chamber of a Dual Chamber unit 408 2 2 1 Remove the top cover from the control housing 408 2 2 2 Label and disconnect the cables that exit the control housing and are routed through the wiring channel on the back of the upper chamber Push slack into the wiring channel 408 2 2 3 Open the outer door of the lower chamber and remove the single Phillips head screw from the left and right sides underneath the display panel 408 2 2 4 Grasp the display panel and pull it forward swing the lower edge of the display panel away from the cabinet and lift the panel off retaining bar 408 2 2 5 The PCB tray is retained by an L shaped bracket on each side Remove the brackets and their Phillips head screws retain for use later 408 2 2 6 Grasp the PCB tray and pull it straight out to the limit of the harness 408 2 2 7 From the Upper chamber push more wire into the wiring channel 408 2 2 8 Repeat steps 408 2 2 6 and 408 2 2 7 until sufficient clearance is gained to replace the desired component 408 3 Label and disconnect all electrical connections on the printed circuit board being replaced 408 4 Remove the board mounting screws Do not worry that standoffs or nuts will become inaccessible since they are all captive on the electrical deck 408 5 Replace the printe
51. oard and one on the Temperature CO2 RH Compensator Board NOTE Because fuse links can fail near the ends where the break is not visible visual inspections are not always reliable always check fuses with an ohmmeter Fuse Evaluation 405 1 Turn off the main power switch located at the top rear of the chamber Unplug the service cord from the wall outlet 405 2 Using an ohmmeter place the meter leads across the fuse a reading of less than 1 ohm indicates a good fuse a reading of infinite resistance indicates a blown fuse that should be replaced Replacing 405 3 Remove the incubator top cover and suspected fuse from its spring clips can result from breakage if fingers are used for fuse removal use of a non metallic fuse puller is CAUTION Use a non metallic fuse puller Because the fuses are glass enclosed and severe cuts recommended 405 4 Replace the fuse in the reverse order 400 4 Thermo Electron Laboratory Products e Procedure 406 Replacing Solenoid Valves There is normally one solenoid S1 mounted on the electrical deck unless an optional automatic tank switcher has been factory installed A second solenoid valve S2 is used in the tank switcher assembly Replacing 406 1 Turn off the main power switch located at the top rear of the control housing and unplug the service cord from the wall outlet 406 2 Remove the control housing cover and locate the solenoid to be replaced Note that t
52. of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language in any form by any means without the written permission of Thermo Electron Laboratory Products Training Department 02 02 06 Rev E Company name change to Thermo Electron 11 1 02 Rev D Clarified procedures 505 amp 506 regarding loss of AutoZero offset due to power outage 2 8 02 Still Rev C Various alignment issues on pp400 1 700 5 removed typed in page numbers on pp 10 30 01 Rev C Changed company name to Thermo Electron Laboratory Products throughout 4 19 01 Rev B Added reference tables to Appendix CO2 signal amp part numbers and CO2 replacement how to procedure 11 30 00 Rev A Changed company name to Revco Technologies Inc throughout Added signal ground to end of 501 7 1 paragraph alignment on step 501 7 4 added Rh Sensor replacement procedure 407 step 501 8 4 17 00 Corrected PN on front cover from 974 to 977 Created 12 29 1999 mo i and ii Thermo Electron Laboratory Products Table of Contents Section 100 Product Overview ccccceceeeeee ek ee ee ee pg 100 1 Section 200 Constructi0n iese sesse ese dese gee eed Ge ee GR ee de ee pg 200 1 Section 300 Operations sesse Ros eeose ree DEE GER GE EN KS DEE GEN EE pg 300 1 SEIS AE EE OE RA OE pg 300 1 Vi DS EA ER EE EE EE EE EE EE Ne pg 300 2 CO2 CTO AE OE EE RE N EE N pg 300 3 Temperatu
53. pabilities of the microprocessor Strain Relief Fitting Provided for power cord security This is a clamp with two screws which when tightened sufficiently will protect the power cord connections against accidental strain HEPA Filter A circular High Efficiency Particulate Accumulator is fitted into the intake side of the circulating fan via tabs to continuously scrub particulates to protect cultures from airborne contamination A complete air exchange occurs every two minutes Filter should be changed every 2 3 months or if deterioration of CO2 control is observed 200 2 Thermo Electron Laboratory Products Section 300 OPERATION When the incubator is first powered up the alphanumeric display will read HELLO The display will go blank after 10 seconds e Setpoints Temperature The incubator will control from ambient 5 C to 55 C Simultaneously press T m N the SET TEMP key and LU LL either the INCREMENT or E al cum om SWRA DECREMENT key The alphanumeric display will read SET TEMP and the digital display will scroll up or down until you release the increment or decrement key The alphanumeric display will automatically go blank approximately 10 seconds after the last change NOTE The temperature control setpoint must be selected before the Overtemp setpoint can be entered Overtemp This value cannot
54. pin of JP5 Continued 500 5 Thermo Electron Laboratory Products 506 6 506 7 506 8 506 9 Procedure 506 Infra Red CO2 Calibration Cont d Change setpoint to 10 and allow to stabilize for approx 30 minutes Disconnect or pinch off the CO2 supply and take a measurement from the sample port If the reading does not measure 10 0 1 place a jumper on JP2 and press Up or Down button on the IR interface board until the displayed CO value equals the analyzer reading Remove the jumper from JP2 and store it on one pin of JP5 Reconnect the CO2 supply wait ten minutes and re check Repeat J2 adjustment if required Change Setpoint to 5 open fan door 5 seconds then allow unit to stabilize for 30 45 minutes Disconnect or pinch off the CO2 supply and take a measurement from the sample port If the reading does not measure 5 0 1 place a jumper on JP2 and press Up or Down button on the IR interface board until the displayed CO value equals the analyzer reading Remove the jumper from JP2 and store it on one pin of JP5 Reconnect the CO2 supply wait ten minutes and re check Repeat J2 adjustment if required Procedure 507 CO2 Auto Zero Adjustment gt 507 1 507 2 507 3 NOTE When CO2 discrepancies are noted always perform an Auto Zero Procedure 507 followed with a CO2 Offset Procedure 508 NOTE An AutoZero offset value gt 2 9 will be lost if the power is interrupted If the discrepancy bet
55. re Otitis pg 300 4 H midity Did pg 300 5 Section 400 Component Replacement Procedures Display Probe c iss is oie Se Se ES DE GE GEES GEE GES DE GE pg 400 1 Control Probe Single or Upper Chamber 0 00 cceeeeeeeeee eee ee pg 400 2 Control Probe Lower hamer ss sea BES ESE EE BED Ge EN pg 400 3 Chamber Pan oo E RESONEER RE OE ER orca EG Ee Se ee Ee Ge ie pg 400 4 ORE EE EER pg 400 4 Solenoid Val VES ESE SERE idos pg 400 5 Press re SN WINCHES os in oan REEDE oo RE ER DE De GER We RE pg 400 5 Electronic Circuit BOards osse ser RE De GE EDE GEE SE GR Ge RES GREG pg 400 6 Humidity Sensors siese Es ED DEE ER GE DE OE aen iee GES GE RE GR GE ees pg 400 7 CO2 SSMS Se EE EG EE See ee eg pg 400 8 Section 500 Adjustment Procedures Procedure 501 Basic Eleccion pg 500 1 Procedure 502 Chamber temperature Display Offset siese es esse ee pg 500 2 Procedure 503 Water Jacket Temperature Control Offset pg 500 3 Procedure 504 Humidity SenSOT i e sesse ee ERG NE Ee Se pensara Dee pg 500 4 Procedure 505 TC CO2 PCB Calibrations civiasiscscsesasestseveavestends pg 500 4 Procedure 506 IR CO2 PCB Calibration cece ese ee eke eee pg 500 4 Procedure 507 CO2 Auto ZO ai is se Se oe ER Ee DR SR RE ER ENG GR pg 500 5 Procedure 508 CO2 Sensor OfTSet ies Ee EER EES KEER EE ER RE EER EG ds GR Ee pg 500 6 Section 600 PCB Description amp Pin Outs Main Control Board 31664GXxX cec
56. t used DIP switch 3 is not used on version 1 1 was used in the on state to view and or calibrate water temperature on version 1 0 software off is the normal default position DIP switch 4 is on top chamber of a dual incubator DIP switch 5 is off DIP switch 6 is on when RH sensing is present on the unit DIP switch 7 is on when tank switcher is present and off otherwise DIP switch 8 is normally Off On for 8 segment alphanumeric displays see p 700 5 e Main Control Board 31664Gxx Pin Outs JP1 Power Interface 1 12 volts from power board 2 12 volts from power board 3 5 volts from power board 4 Valve Voltage from power board to tank 5 Switched Ground to Fan Relay on power board switcher valve 10 7 11 3 V 6 Switched Ground to Main Heater 7 Switched Ground to Door Heater 8 Switched Ground to CO2 solenoid 9 Ground 10 Ground JP2 RS 232 Output to DB9 connector on incubator 1 Receive 2 Transmit 3 Ground JP3 Display Cable Output NOTE Pin 1 is denoted by arrow on board See Display Board Description Section 600 2 for cable description 600 1 Thermo Electron Laboratory Products Main Control Board 31664Gxx Pin Outs Cont d JP4 CO Input from T C or IR boards PCB 1 Signal 0 1 3Vdc 2 Signal 3 n c 4 Ground JP6 Chamber Temperature Sensor Input 1 Signal sense 2 Ground 3 Shield 4 n c JP8 Water Temperature Sensor Input 1 Signal
57. ta from the newest 4000 records If a temperature data field appears blank on the screen for a period of 10 seconds while the message Communication error is not displayed a communication problem between computer and incubator may exist or the incubator sensor has developed a fault Turn off the incubator and computer for about 30 seconds and restart the program The temperature displayed on the computer must match the temperature displayed on the incubator user interface There is up to a one second delay between the computer screen and the incubator user interface If the computer attempts to communicate with the incubator microcomputer and receives no response after three tries the statement Communication Error is saved to disk at the appropriate logging interval and the similar message is displayed to the screen A sample of the data structure which is logged to disk is given below Field 1 2 3 4 5 6 7 8 9 date time co2 co2 s temp temp s ov tem rh count 09 29 1992 12 00 45 005 0 005 0 037 0 037 0 037 5 098 2 0001 09 29 1992 12 01 45 005 0 005 0 037 0 037 0 037 5 098 2 0002 09 29 1992 12 02 45 005 0 005 0 037 0 037 0 037 5 098 2 0003 09 29 1992 12 03 45 005 0 005 0 037 0 037 0 037 5 098 2 0004 09 29 1992 12 04 45 005 0 005 0 037 0 037 0 037 5 098 2 0005 09 29 1992 12 05 45 005 0 005 0 037 0 037 0 037 5 098 2 0006 09 29 1992 12 06 45 005 0 005 0 037 0 037 0 037 5 098 2 0007 Field 1 is date Field 2 is time Field 3 is CO reading Field 4
58. ting CO2 setpoint and allow the unit to stabilize forty five 45 minutes Measure the CO2 content and compare to the CO2 setpoint Adjust the CO2 board potentiometer VR3 Span until the CO2 display matches the Measured content CAUTION if the Measured content is less than the SET CO2 value then disconnect the CO2 solenoid J4 before adjusting VR3 6B Connect the CO2 solenoid at J4 and set the Set CO2 to 20 and allow the unit to stabilize forty five 45 minutes Measure the CO2 content and compare to the CO2 setpoint Adjust the CO2 board potentiometer VR3 Span until the CO2 display matches the Measured content CAUTION if the Measured content is less than the SET CO2 value then disconnect the CO2 solenoid J4 before adjusting VR3 7 Repeat steps 2 5 until the unit holds 5 above the desired operating level of CO2 8 Repeat steps 2 4 9 Connect the CO2 solenoid set the Set CO2 to the desired operating level of CO2 and allow unit to stabilize forty five 45 minutes Measure the CO2 content and compare with SET CO2 Adjust the SET ZERO potentiometer on the front panel until the CO2 display matches the measured content CAUTION if the Measured content is less than the SET CO2 value then disconnect the CO2 solenoid J4 before adjusting VR3 10 Repeat Steps 8 and 9 one time 11 On the Main Control board turn off DIP switch 1 calibration is complete 700 8 Thermo Electron Laboratory Products INFRA RED CO2 CO
59. utside this range 501 7 3 Measure the voltage between Main Control Board TP2 and Power Board GND as above 501 7 4 Adjust R21 on the Main Control Board if the voltage is outside the above specification 501 8 Relative Humidity Zero Calibration if Option is present 501 8 1 If unit has had water in the humidity pan allow to stabilize overnight without water in the humidity pan 501 8 2 Depress only the RH button on the control panel The RH reading will appear on the display 501 8 3 If the reading on the display differs from the RH test instrument by more than 2 adjust R4 Zero potentiometer on either the 33686H02 board IR sensor systems or the 33686H01 board TC sensor systems CAUTION Do NOT attempt to adjust R6 as it will result in a board return for calibration at the circuit board manufacturer 501 9 Verify Alarm Terminals 501 9 1 Create a temperature alarm condition by lowering the SET OVERTEMP below the displayed Temperature value 501 9 2 Verify that depressing the MUTE button can silence the audio alarm verify that the red LED next to the MUTE button lights 501 9 3 With your test meter set on Ohms check remote alarm terminal screws With probes on terminals 1 and 2 and unit in an alarm condition should measure 0 0 Ohms 501 9 4 Place you meter probes on 1 and 3 Now with the alarm condition existing there should be an OPEN or co reading 501 10 Verify Heater Operation 501 10 1 Adjust SET TEMP to 60c to ensure
60. ween Measured and Displayed CO2 value is greater than 2 5 perform board calibration Procedure 505 or 506 Open and fan the glass door for thirty 30 seconds to eliminate CO2 from the chamber With the glass door open depress the front panel CALIBRATE key to begin the Auto Zero procedure observe AUTO ZERO is indicated on the Status display NOTE If the door is closed before CLOSE DOOR message is displayed or if the door is opened before the Auto Zero is completed a ZERO ERROR message will appear and the Auto Zero is aborted To clear the message press CALIBRATE key NOTE The Auto Zero function will last for five 5 to thirty 30 minutes and will be complete when the Auto Zero message is no longer displayed and the CO window resumes displaying the CO content Close the glass and outer doors when the status display indicates CLOSE DOOR Auto Zero is complete 500 6 Thermo Electron Laboratory Products Procedure 508 CO2 Sensor Offset Adjustment LS 508 1 508 2 508 3 508 4 508 5 NOTE When CO2 discrepancies are noted always perform an Auto Zero Procedure 507 followed with this CO2 Offset Disconnect or pinch off the CO2 supply Connect a CO2 analyzer e g Bacharach Fyrite IR detector etc to the 1 8 barbed fitting on the cabinet front and measure the CO2 content of the chamber If the reading on the analyzer differs from the readout on the display panel press the CALIBRATE key twice O
61. y Talk to a live person 888 88 SOURCE 888 887 6872 Contact us by email sales artisan scientific com Visit our website http www artisan scientific com
62. y The alphanumeric y n Inn a display will read SET OV TEM As E VULL above the display will go blank after s 10 seconds CO Setpoint Press the SET CO2 key The CO digital display will read the CO cor os setting and the alphanumeric display 30 JANE RE will read SET CO2 and then go am blank as above RH Relative Humidity optional feature Press the RH key if present The Temperature display will read the humidity value View Offsets Viewing offsets is handy for the Service Technician in troubleshooting Since the range of offsets available is limited you may have difficulty changing offsets if the maximum or minimum has been set When placing a call to the factory for assistance relative to calibration please have the offset values available as they may be helpful in diagnosis CO Simultaneously press SET CO2 and SET TEMP keys The alphanumeric display will read VIEW C OFS STATUS EIE SE A Temperature Simultaneously press SET OVERTEMP and in Ee SET CO2 keys The q At tat ener alphanumeric display will sn show VIEW T OFS

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