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Agilent 1200 Series Isocratic Pump User Manual

1. 1 The vacuum degasser will have a special connector for specific use For details see the degasser manual 2 The interface board slot not common to all modules provides specific interfacing needs external con tacts BCD LAN and so on 16 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 2 Site Requirements and Specifications Site Requirements 18 Power Consideration 18 Power Cords 19 Bench Space 20 Environment 20 Physical Specifications 21 Performance Specifications 22 gy Agilent Technologies 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the instrument Power Consideration The module power supply has wideranging capability see Table 3 on page 21 It accepts any line voltage in the range described in the above mentioned table Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to p
2. gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series Isocratic Pump User Manual 19 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 3 on page 21 allow to place the module on almost any laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for the circulation of air and electric connections If the bench should carry a complete Agilent 1200 Series system make sure that the bench is designed to carry the weight of all the modules The module should be operated in a horizontal position Environment Your module will work within specifications at ambient temperatures and relative humidity as described in Table 3 on page 21 CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 20 1200 Series Isocratic Pump User Manual Site Requirements and Specifications 2 Physical Specifications Table3 Physical Specifications Type Specification Comments Weight 11 kg 25 Ibs Dimensions
3. For more information see the User Manual for the Agilent 1200 Series vacuum degasser 1200 Series Isocratic Pump User Manual Optimiziming Performance 5 When to use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and plungers in your pump The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent The seal wash option is strongly recommended when buffer concentrations of 0 1 Molar or higher will be used for long time periods in the pump The seal wash option can be ordered by quoting part number 01018 68722 kit contains all parts needed for one pump head The active seal wash option kit can be ordered by quoting part number G1311 68711 The seal wash option comprises a support ring secondary seal gasket and seal keeper for both plunger sides A wash bottle filled with water isopropanol 90 10 should be placed above the pump in the solvent cabinet and gravity will maintain a flow through the pump head removing all possible buffer crystals from the back of the pump seal For the active seal wash a peristaltic pump is pumping the solvent through the pump head Running dry is the worst case for a seal and drastically reduces its lifetime The seal will build up sticky layers on the surface of the plunger These sticky layers will also reduce the lifetime of the primary seal Therefore the tubes of the wash option should always be
4. Installing the Pump 3 Connecting an Agilent 1200 Series Vacuum Degasser 1 Place the vacuum degasser in the stack of modules as shown in Figure 5 on page 28 2 Ensure the power switch on the front of the vacuum degasser is OFF switch stands out 3 Plug an APG cable into the APG remote connector at the rear of the module 4 Connect the APG cable to the APG remote connector of the pump see Figure 6 on page 29 5 Press in the power switches to turn on the vacuum degasser The AUX output allows the user to monitor the vacuum level in the degasser chamber Connecting control software and or control modules 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 Plug a GPIB cable into the GPIB connector at one of the modules preferably at the detector MUST for the DAD 3 Connect the GPIB cable to the Agilent control software in use 4 Plug a CAN cable into the CAN connector of the control module Do not connect the Agilent control software or the control module with the vacuum degasser 5 Connect the CAN cable to the CAN connector of one of the modules 6 Press in the power switches to turn on the modules 1200 Series Isocratic Pump User Manual 35 3 36 Installing the Pump The Agilent control software e g ChemStation EZChrom OL etc can be also be connected to the system through a LAN cable which requires the installation of a LAN board For more information a
5. 1 5022 2159 Restriction capillary Preparations Switch off pump at the main power switch Remove the front cover Removing the Pump Head Assembly on page 82 1 Place the pump head on a flat surface Loosen the lock 2 Remove the support rings from the plunger housing and screw two revolutions and while holding the lower half lift the housing away from the plungers of the assembly carefully pull the pump head away from the plunger housing ca _ eS p 6 eS gt es il i Support ring SS __ Pump head Mt eS A A SBS A i ba m SN Lock screw poa ee SSN Pl nger housing 7 Plunger housing Plunger 1200 Series Isocratic Pump User Manual 83 7 Maintenance Simple Repairs 3 Using one of the plungers carefully remove the seal from the pump head be careful not to break the plunger Remove wear retainers if still present 4 Insert new seals into the pump head Pump head Support ring Plunger housing 84 1200 Series Isocratic Pump User Manual Maintenance 7 Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 but it will definitely damage the normal phase application seals 0905 1420 1 Place a bottle with 100 ml of isopropanol in the solvent cabinet and place the tubing including bottle head assembly of the pump head that is supposed to be worn in into the bottle 2 Screw the adapter 0100 18
6. Outlet capillary Waste tube Figure 19 Exchanging the Purge Valve 1200 Series Isocratic Pump User Manual 81 7 Maintenance Removing the Pump Head Assembly When Tools required Preparations CAUTION Exchanging the seals Exchanging the plungers Exchanging seals of the seal wash option Wrench 1 4 inch 4 mm hexagonal key Switch off pump at the main power switch and unplug the power cable Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed 82 1 Remove the front cover 2 Disconnect the Active Inlet Valve cable 3 Using a 1 4 inch wrench remove the outlet capillary 4 Disconnect the capillary from the Outlet Ball Valve 5 Remove the waste tubing and disconnect the Active Inlet Valve tubing 6 Remove the capillary at the bottom of the Pumphead 7 Using a4 mm hexagonal key stepwise loosen the two Pumphead screws and remove the Pumphead from the Pump Drive 1200 Series Isocratic Pump User Manual Maintenance 7 Exchanging the Pump Seals and Seal Wear in Procedure When Seal leaking if indicated by the results of the leak test Tools required Wrench 1 4 inch 4 mm hexagonal key Parts required Part number Description 2 5063 6589 standard Seals pack of 2 or 0905 1420 Seals pack of 2 for normal phase applications 1 0100 1847 For the seal wear in procedure Adapter AIV to inlet tube
7. Zero 50 Att 2 3 Chart Speed 10 cm min 4 Start the recording device with the plot mode Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition 1200 Series Isocratic Pump User Manual 55 5 56 Optimiziming Performance Table8 Solvent Compressibility Solvent pure Compressibility 10 6 bar Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol lsopropanol Methanol 1 Propanol Toluene Water 126 115 95 110 100 118 114 104 120 150 100 100 120 100 87 46 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 58 Overview of the Pump s Indicators and Test Functions 59 Status Indicators 60 Power Supply Indicator 60 Instrument Status Indicator 61 User Interfaces 62 ee Agilent Technologies 57 Troubleshooting and Diagnostics Agilent Lab Advisor Software 58 The Agilent Lab Advisor Software is a standalone product that can be used with or without data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs
8. for normal phase applications 0905 1420 9 Pump chamber housing G1311 25200 10 Active inlet valve body G1312 60025 Replacement cartridge for active inlet valve 400 bar 5062 8562 11 Outlet ball valve complete with cartridge G1311 60012 12 Screw lock 5042 1303 13 Purge valve G1311 60009 14 Screw M5 60 mm lg 0515 2118 15 Seal wash pump assembly 5065 9953 Seal wash upgrade kit see Seal Wash Option Kit 01018 68722 G1311 68711 on page 112 106 1200 Series Isocratic Pump User Manual Parts and Materials for Maintenance 8 Pump Head Assembly with Seal Wash Option Figure 28 Pump Head with Seal Wash Option 1200 Series Isocratic Pump User Manual 107 Parts and Materials for Maintenance Outlet Ball Valve Assembly 108 Table 15 Outlet Ball Valve Assembly Item Description Part Number Outlet ball valve complete assembly G1311 60012 1 Socket cap 5042 1345 2 Outlet valve housing screw 01018 22410 3 Gold seal outlet 5001 3707 4 Cap pack of 4 5062 2485 MAN an E E L f f f NS Figure 29 Outlet Ball Valve Assembly 1200 Series Isocratic Pump User Manual Parts and Materials for Maintenance 8 Purge Valve Assembly Table 16 Purge Valve Assembly Item Description Part Number Purge valve complete assembly G1311 60009 1 Valve body No part number 2 PTFE frit pack of 5 01018 22707 3 Gold seal 5001 3707 4 Cap pack of 4 5062 2485 Figure 30 Purge Valve Asse
9. 2190 0409 1200 Series Isocratic Pump User Manual 103 Parts and Materials for Maintenance Pump Head Assembly 104 Table 13 Pump Head Assembly Item Description Part Number z a 7 T 5 Complete assembly including items marked with Sapphire plunger Plunger housing including spring Support ring Seal pack of 2 or Seal pack of 2 for normal phase applications Pump chamber housing Active inlet valve body Replacement cartridge for active inlet valve 400 bar Outlet ball valve Screw lock Purge valve assembly Screw M5 60 mm lg G1311 60004 5063 6586 G1311 60002 5001 3739 5063 6589 0905 1420 G1311 25200 G1312 60025 5062 8562 G1311 60012 5042 1303 G1311 60009 0515 2118 1200 Series Isocratic Pump User Manual Parts and Materials for Maintenance 8 Pump Head Assembly Figure 27 Pump Head Assembly 1200 Series Isocratic Pump User Manual 105 8 Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option Table 14 Pump Head Assembly with Seal Wash Option Item Description Part Number Complete assembly including parts marked with G1311 60005 1 Sapphire plunger 5063 6586 2 Plunger housing including spring G1311 60002 3 Support ring seal wash 5062 2465 4 Secondary seal 0905 1175 5 Wash tube 1 0 m 0890 1764 6 Gasket seal wash pack of 6 5062 2484 7 Seal keeper 5001 3743 8 Seal pack of 2 or 5063 6589 Seal pack of 2
10. Plungers on page 86 Installing the Seal Wash Option on page 88 Exchanging the Optional Interface Board on page 96 If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If pump performance indicates seal wear If scratched If seals show indication of leaks If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit 5 ml min H30 with purge open indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification Seal life time shorter than normally expected check plungers while changing the seals Leaks at lower pump head side loss of wash solvent Error condition indicated by red status indicator 1200 Series Isocratic Pump User Manual 71 7 Maintenance Checking and Cleaning the Solvent Filter CAUTION Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the
11. Reduce the Algae Problem 50 gy Agilent Technologies 45 4 Using the Isocratic Pump Hints for Successful Use of the Pump 46 Always place solvent cabinet with the solvent bottle on top of the pump or at a higher level When using the pump without vacuum degasser shortly degass your solvents Vacuum pump the solvents for 15 30 s in an appropriate vessel before using them in the pump If possible apply solvent conditions that will decrease the gas solubility for example warming up the solvents For highest precision and reproducibility use vacuum degasser When using the pump with vacuum degasser before operating the pump flush the degasser with at least two volumes 30 ml especially when turned off for a certain length of time for example during the night and volatile solvent mixtures are used in the channels see Priming and Purging the System on page 40 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 48 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 ml min with an open purge valve When using the pump at low flow rates for example 0 2 ml min check all 1 16 inch fittings for a
12. and LCs configured on the lab intranet Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200 Series HPLC modules This includes tests and calibrations procedures as well as the different injector steps to perform all the maintenance routines Agilent Lab Advisor also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor Software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 1200 Series Isocratic Pump User Manual Troubleshooting and Diagnostics 6 Overview of the Pump s Indicators and Test Functions Status Indicators The pump is provided with two status indicators which indicate the operational state prerun run and error states of the pump The status indicators provide a quick visual check of the operation of the pump see Status Indicators on page 60 Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For details on error messages and error handling please refer to the Agilent Lab Monitor amp Diagn
13. in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump e Small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure e Column filters to plug giving high system pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common Symptoms Observed with the Agilent 1200 Series HPLC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing algae have a better chance to grow in systems such as the Agilent 1200 Series where helium is not used for degassing most algae need oxygen and light for growth The presence of algae in the Agilent 1200 Series can cause the following to occur e PTFE frits part number 01018 22707 purge valve assembly and column filter blockage causing increased system pressure Algae appear as
14. of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 1200 Series Isocratic Pump User Manual 117 9 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 118 Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1200 Series Isocratic Pump User Manual Appendix 9 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste dispo
15. the isocratic pump The pump head and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacing internal parts will require to remove the isocratic pump from its stack and to open the top cover Figure 13 Overview of Repair Procedures 1 Active inlet valve Exchanging the Active Inlet Valve on page 73 2 Purge valve Exchanging the Purge Valve Frit or the Purge Valve on page 79 1200 Series Isocratic Pump User Manual 69 7 Maintenance arview of Maintenance and Repair 3 Outlet ball valve Exchanging the Outlet Ball Valve on page 77 4 Pump head Removing the Pump Head Assembly on page 82 5 Pump drive 6 Power supply 7 LPM board 8 Fan 9 Damping unit 10 Leak sensor 70 1200 Series Isocratic Pump User Manual Simple Repairs Maintenance 7 The procedures described in this section can be done with the isocratic pump in place in the system stack Table 10 Simple Repair Procedures Procedure Typical Frequency Notes Checking and Cleaning the Solvent Filter on page 72 Exchanging the Active Inlet Valve on page 73 Exchanging the Outlet Ball Valve on page 77 Exchanging the Purge Valve Frit or the Purge Valve on page 79 Exchanging the Purge Valve Frit or the Purge Valve on page 79 Exchanging the Pump Seals and Seal Wear in Procedure on page 83 Exchanging the
16. 0 Series Isocratic Pump User Manual 95 7 Maintenance Exchanging the Optional Interface Board When Parts required CAUTION Board defective Part number Description 1 G1351 68701 Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 1 Switch off the pump at the main power switch unplug the pump from line power 2 Disconnect cables from the interfaceboard connectors 3 Loosen the screws Slide out the interface board from the pump 4 Install the new interface board Secure screws 5 Reconnect the cables to the board connector 6 Reconnect the pump to line power x NS N BCD Interface board 96 Figure 24 Exchanging the Interface Board 1200 Series Isocratic Pump User Manual Maintenance 7 Replacing the Module s Firmware When Tools required Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be n
17. 1311 68711 112 gy Agilent Technologies Parts and Materials for Maintenance Overview of Main Assemblies Overview of Main Assemblies Overview of Main Assemblies Front View Figure 25 1200 Series Isocratic Pump User Manual 100 Parts and Materials for Maintenance Table 11 Repair Parts Pump Housing and Main Assemblies Front View Item Description Part Number 1 Pump head see Pump Head Assembly on page 104 2 Pump drive assembly Exchange assembly pump drive 3 Cable assembly AIV to main board 4 Low pressure pump main board LPM Exchange assembly LPM board 5 Fan assembly 6 Damping unit 7 Leak pan pump G1311 60004 G1311 60001 G1311 69001 G1311 61601 G1311 66520 G1311 69520 3160 1016 79835 60005 5042 8590 1200 Series Isocratic Pump User Manual 101 8 Parts and Materials for Maintenance Overview of Main Assemblies Se y LA me Figure 26 Overview of Main Assemblies Rear View 102 1200 Series Isocratic Pump User Manual Parts and Materials for Maintenance Table 12 Repair Parts Pump Housing and Main Assemblies Rear View Item Description Part Number 1 Nut M14 analog output 2940 0256 2 Washer analog output 2190 0699 3 Standoff remote connector 1251 7788 4 Standoff GPIB connector 0380 0643 5 Power supply behind rear panel 0950 2528 Screw M4 7 mm lg power supply 0515 0910 7 Washer power supply
18. 140 x 345 x 435 mm 5 5 x 13 5 x 17 width x depth x height Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL inches 100 240 VAC 10 50 or 60 Hz 5 180 VA 55 W 188 BTU 4 55 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6500 ft Up to 4600 m 14950 ft Installation Category II Pollution Degree 2 Wide ranging capability Maximum Non condensing For storing the module For indoor use only Research Use Only Not for use in Diagnostic Procedures 1200 Series Isocratic Pump User Manual 21 2 Site Requirements and Specifications Performance Specifications 22 Table4 Performance Specification Agilent 1200 Series Isocratic Pump Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compensation Recommended pH range Control and data evaluation Analog output Communications Safety and maintenance Specification Dual piston in series pump with proprietary servo controlled variable stroke drive floating pistons and active inlet valve 0 001 10 ml min in 0 001 ml min increments 0 2 10 0 ml min lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retent
19. 23 vacuum degasser 6 46 valve frit 79 variable stroke volume 12 variable reluctance motor 8 velocity regulator 27 voltage range 21 WwW waste tube 27 weight 21 wrench 1 4inch 77 79 82 82 83 83 86 86 92 92 wrench 1 4inch 95 95 wrench 14mm 73 75 77 79 wrench 1 4 5 16inch 27 wrench 14mm 27 1200 Series Isocratic Pump User Manual 127 www agilent com In This Book This manual contains technical information about the Agilent 1200 Series isocratic pump The manual describes the following e introduction e site requirements and specifications e installing the pump e using the isocratic pump e optimizing performance e troubleshooting and diagnostics e maintenance e parts and materials for maintenance e appendix Agilent Technologies 2006 2007 2008 Printed in Germany 11 08 G1310 90011 fe Agilent Technologies
20. 47 to the AIV and connect the inlet tube from the bottle head directly to it 3 Connect the restriction capillary 5022 2159 to the purge valve Insert its other end into a waste container 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min 5 Close the purge valve set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 minutes at this pressure to wear in the seals The pressure can be monitored at your analog output signal with the handheld controller Chemstation or any other controlling device connected to your pump 6 Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reconnect the outlet capillary at the purge valve and the connecting tube from solvent selection valve if installed to the AIV 7 Rinse your system with the solvent used for your next application 1200 Series Isocratic Pump User Manual 85 7 Maintenance Exchanging the Plungers When When scratched Tools required Wrench 1 4 inch 4 mm hexagonal key Parts required Part number Description 1 5063 6586 Plunger Preparations Switch off pump at the main power switch Remove the front cover Removing the Pump Head Assembly on page 82 1 Disassemble the Pumphead assembly _ lt Support ring SS Plunger housing Plunger 2 Check the plunger surface and
21. 52 PTFE frit 79 PTFE frit 27 pump head assembly 104 pump head with seal wash 106 pump piston 46 pump seals 46 purge valve 79 purge valve 46 p urging the pump 40 radio interference 120 reassembling the pump head 95 recommended pH range 22 removing pump head assembly 82 repair procedures 71 repairs replacing firmware 97 using the ESD strap 66 reproducibility 46 runmode 61 S safety class 114 safety information lithium batteries 119 1200 Series Isocratic Pump User Manual Index safety general information 114 standards 21 symbols 117 sapphire piston 8 seal insert tool 27 sealwash 7 53 seal wear counters 67 seal wash installing 88 whentouse 53 seal alternative material 54 seals 46 83 86 88 88 92 95 security lever 32 64 setable flow range 22 shipping containers 26 simple repair procedures 71 simple repairs 64 site requirements 18 snap fasteners 37 solvent filters cleaning 72 solvent inlet filters 46 solvent bottle 26 solvent cabinet 26 46 solvent filters checking 72 prevent blocking 48 solvent information 47 122 solvents 122 sonic bath 77 specification physical 21 stack configuration front view 28 stack configuration rearview 29 stack configuration 28 status indicator 59 60 status lamp 60 stroke volume 8 stroke volume 12 syringe adapter 41 U unpacking the pump 21 26 V vacuum degasser operational hints 52 vacuum degasser when to use 52 vacuum degasser
22. Agilent 1200 Series Isocratic Pump User Manual ott Agilent Technologies Notices Agilent Technologies Inc 2006 2007 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61310 90011 Edition 11 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms i
23. Cl Op gt 2COCly 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 1200 Series Isocratic Pump User Manual Appendix 9 Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF 1200 Series Isocratic Pump User Manual 123 9 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download 124 1200 Series Isocratic Pump User Manual Index Index A accessory kit 27 active inlet valve 73 110 active sealwash 6 53 88 installing 88 adapter 73 76 Agilent Diagnostic Software 58 Lab Advisor Software 58 oninternet 124 algae growth 48 algae 46 122 122 alternative seal material 54 ambient operating temperature 21 ambient non operating temperature 21 analog output 22 AUTO mode 12 AUX output 35 ball screw drive 8 battery safety information 119 bench space 20 bottle head assembly 26 bottle 26 buffer solutions 6 buffer ap
24. Pump on page 37 7 Press power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off page 40 NOTE The pump was shipped with default configuration settings To change these settings refer to sevice manual Setting the 8 bit configuration switch 8 Purge the isocratic pump see Priming and Purging the System on 1200 Series Isocratic Pump User Manual 33 3 Installing the Pump Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Agilent 1200 Series modules 1 Place the individual modules in a stack configuration as shown in Figure 5 on page 28 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 6 on page 29 5 Press in the power switches to turn on the modules 34 1200 Series Isocratic Pump User Manual
25. Seal N A ik x Support ring SAAS 2 c 6 Place the support rings on the plunger housing plungers not installed and snap the pump head and plunger housing together Pump head Support ring Ss Plunger housing 1200 Series Isocratic Pump User Manual 93 7 Maintenance Simple Repairs Plunger Ca T 7 Insert the plungers and carefully press them into the seal iS 8 Tighten the lock screw Lock screw torque Balls of spindle drive Metering drive Pump head ae a N Pumphead screws 9 Slide the pump head assembly onto the metering drive Apply a small amount of pump head grease to the pumphead screws and the balls of the spindle drive Tighten the pumphead screws stepwise with increasing 10 Reconnect all capillaries tubes and the active inlet valve cable to its connector 94 1200 Series Isocratic Pump User Manual Maintenance 7 Reinstalling the Pump Head Assembly When When reassembling the pump Tools required 4 mm hexagonal key Parts required Description 79846 65501 Pump head grease 1 Apply a small amount of grease on the back of the pistons 2 Slide the Pumphead Assembly onto the Pumpdrive 3 Using a 4 mm hexagonal key tighten the Pumphead screws stepwise with increasing torque 4 Reconnect the capillaries tubing and the Active Inlet Valve cable to the connector 5 Reinstall the front cover 120
26. ate product for applications that require best flow stability especially at low flow rates or highest detector sensitivity This is most likely required to run small internal diameter columns 2 mm and mm i d which require low flow rates A solvent cabinet provides enough space for up to four one liter bottles An active seal wash optional is available when the pump is used with concentrated buffer solutions Power supply LPM board Pump drive Pump head Outlet ball valve Purge valve Active inlet valve Damper Figure 1 Overview of the Isocratic Pump 6 1200 Series Isocratic Pump User Manual Introduction 1 Overview of the Hydraulic Path Overview of the Hydraulic Path Damper Purge valve Outlet ball valve Pump head Active inlet valve 1200 Series Isocratic Pump User Manual The isocratic pump is based on a two channel dual plunger in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by one pump assembly which can generate pressure up to 400 bar The pump assembly includes a pump head with an active inlet valve which has a replaceable cartridge and an outlet valve A damping unit is connected between the two plunger chambers A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head An active seal wash optional is available whe
27. atic Pump User Manual Maintenance 7 Simple Repairs 17 Route the outlet of the wash tube into a waste container Figure 23 Pumphead after completed installation 1200 Series Isocratic Pump User Manual 91 7 Maintenance Exchanging the Wash Seals When When maintaining seal wash option Tools required 4 mm hexagonal key Parts required Part number Description i 0905 1175 Wash seal 6 5062 2484 Gasket seal wash pack of 6 Switch off pump at the main power switch Remove the front cover Preparations 1 Disconnect all capillaries and tubes from the pump head 2 Using a 4 mm hexagonal key stepwise loosen and remove and disconnect the active inlet valve cable the two pump head screws and remove the pump head from the pump drive Pump head screws 92 1200 Series Isocratic Pump User Manual Maintenance 7 3 Remove the seal keeper and the seal wash support rings from the plunger housing Remove the seal keeper from the support ring assembly Seal keeper SS lt E Seal wash support ring en Plunger housing i Plunger 4 Using the blade of a flat blade screwdriver remove the seal wash gasket and the secondary seal from the support ring Secondary seal Seal wash support ring 5 Using the insert tool press the secondary seal spring pointing upwards into the recess of the support ring Place a seal wash gasket in the recess of the support ring Insert tool
28. bout connecting the control module or Agilent control software refer to the respective user manual For connecting the Agilent 1200 Series equipment to non Agilent 1200 Series equipment see Introduction to the Isocratic Pump on page 6 1200 Series Isocratic Pump User Manual Installing the Pump 3 Flow Connections of the Isocratic Pump Tools required Two wrenches 1 4 5 16 inch for capillary connections Parts required Description Other modules Parts from accessory kit see Accessory Kit Contents G1311 68705 on page 27 Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides n RY E I N an Figure 9 Removing the Front Corver 2 Place the solvent cabinet on top of the module 1200 Series Isocratic Pump User Manual 37 3 38 Installing the Pump Place the bottle containing your solvent into the solvent cabinet and place the bottle head assembly into the bottle Connect the solvent tube from the bottle head assembly
29. cation 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 ml min 2 Flush the vacuum degasser and all tubes with at least 30 ml of solvent 3 Set flow to required value of your application and close the purge valve 4 Pump for approximately 10 minutes before starting your application Table 7 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between lsopropanol Best solvent to flush air out of reverse phase and normal phase both times After an installation To clean the system when using buffers After a solvent change After the installation of normal phase seals P N 0905 1420 Ethanol or Methanol Bidistilled water Bidistilled water Hexane 5 lsopropanol the system Alternative to lsopropanol second choice if no Isopropanol is available Best solvent to re dissolve buffer cristals Best solvent to re dissolve buffer cristals Good wetting properties 1200 Series Isocratic Pump User Manual 43 3 44 Installing the Pump Priming and Purging the System 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 4 Using the Isocratic Pump Hints for Successful Use of the Pump 46 Solvent Information 47 Prevent Blocking of Solvent Filters 48 Algae Growth in HPLC Systems 49 How to Prevent and or
30. cond plunger pump unit The outlet of the purge valve assembly is then connected to the following chromatographic system 1200 Series Isocratic Pump User Manual Introduction 1 Overview of the Hydraulic Path Chamber 1 Chamber 2 Purge valve To mixing chamber gt Outlet Inlet valve To waste From solvent bottle Seal Plunger 1 Plunger 2 Ball screw drive Gear Motor with encoder Figure 3 Principle of the Isocratic Pump When turned on the isocratic pump runs through an initialization procedure to determine the upper dead center of the first plunger The first plunger moves slowly upwards into the mechanical stop of the pump head and from there it moves back a predetermined path length The controller stores this plunger position in memory After this initialization the isocratic pump starts operation with the set parameters The active inlet valve is opened and the down moving plunger draws solvent into the first pump head At the same 1200 Series Isocratic Pump User Manual 9 1 10 Introduction time the second plunger is moving upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motor is stopped and the active inlet valve is closed The motor direction is reversed and moves the first plunger up until it reaches the stored upper limit and at the same time moving the second plunger downwards Then the sequence starts again moving the
31. ctive earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged e
32. d can be configured with the configuration switch next to the GPIB connector See your software documentation for further information The power input socket accepts a line voltage of 100 120 or 220 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents the module cover from being taken off when line power is still connected 1200 Series Isocratic Pump User Manual Introduction Electrical Connections Security lever Slot for interface board Analog pressure 2mV bar APG Remote RS 232C Configuration switch Figure 4 Rear View of Pump Electrical Connections and Label 1200 Series Isocratic Pump User Manual 1 15 1 introduction Agilent 1200 Series Interfaces The Agilent 1200 Series modules provide the following interfaces Table 2 Agilent 1200 Series Interfaces Interface Type Pumps Autosampler DA Detector VW Detector Thermostatted Vacuum MW Detector RI Detector Column Degasser FL Detector Compartment CAN Yes Yes Yes Yes Yes No GPIB Yes Yes Yes Yes Yes No RS 232C Yes Yes Yes Yes Yes No APG Remote Yes Yes Yes Yes Yes Yes Analog Yes No 2x 1x No Yes Interface board Yes Yes Yes Yes No No
33. d disconnect the active inlet valve cable 7 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws and remove the pump head from the pump drive 8 Place the pump head on the backside of the plungerhousing on a flat surface Loosen the lock screw two revolutions and while holding the lower half of the assembly carefully pull the pump head away from the plunger housing 9 Remove the support rings from the plunger housing and lift the housing away from the plungers 1200 Series Isocratic Pump User Manual 89 7 Maintenance 10 Install the support ring assembly from the active seal wash option kit into the plunger housing Figure 22 Inserting the active seal wash support rings 11 Place the support rings on the plunger housing plungers not installed and snap the pump head and plunger housing together 12 Insert the plungers and carefully press them into the seal 13 Tighten the lock screw 14 Slide the pump head assembly onto the metering drive Apply a small amount of pump head grease part number 79846 65501 to the pumphead screws and the balls of the spindle drive Tighten the pumphead screws stepwise with increasing torque 15 Reconnect all capillaries tubes and the active inlet valve cable to its connector 16 Route the wash inlet tube into a bottle filled with a mixture of distilled water and isopropanol 90 10 and place the bottle above the pump in the solvent cabinet 90 1200 Series Isocr
34. dicators and Test Functions 59 Status Indicators 60 User Interfaces 62 Maintenance 63 Introduction to Maintenance and Repair 64 Early Maintenance Feedback EMF 67 Overview of Maintenance and Repair 69 Simple Repairs 71 Parts and Materials for Maintenance 99 Overview of Main Assemblies 100 Pump Head Assembly 104 Pump Head Assembly with Seal Wash Option 106 Outlet Ball Valve Assembly 108 Purge Valve Assembly 109 Active Inlet Valve Assembly 110 Accessory Kit G1311 68705 111 Seal Wash Option Kit G1311 68711 112 Appendix 113 General Safety Information 114 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 118 Lithium Batteries Information 119 Radio Interference 120 Sound Emission 121 Solvent Information 122 Agilent Technologies on Internet 124 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 1 Introduction Introduction to the Isocratic Pump 6 Overview of the Hydraulic Path 7 How Does the Pump Work 8 How Does Compressibility Compensation Work 11 How Does Variable Stroke Volume Work 12 Early Maintenance Feedback EMF 12 Instrument Layout 13 Electrical Connections 14 Agilent 1200 Series Interfaces 16 ee Agilent Technologies 1 introduction Introduction to the Isocratic Pump Introduction to the Isocratic Pump The isocratic pump comprises a pump assembly and a damping unit Degassing is not included but avacuum degasser is available as a separ
35. ecessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third part control software requires a special version LAN RS 232 Firmware Update Tool or Agilent Lab Monitor amp Diagnostic Software LMD Instant Pilot G4208A only if supported by module Control Module G1323B only if supported by module Description Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware the following steps have to be performed 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 Load the firmware into the module as described in the documentation Due to a different hardware platform there is no way to convert a G1314D VWD and the G1314E VWD SL Plus to a G1314A B VWD or G1314C VWD SL 1200 Series Isocratic Pump User Manual 97 7 Maintenance Simple Repairs 98 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 8 Parts and Materials for Maintenance Overview of Main Assemblies 100 Pump Head Assembly 104 Pump Head Assembly with Seal Wash Option 106 Outlet Ball Valve Assembly 108 Purge Valve Assembly 109 Active Inlet Valve Assembly 110 Accessory Kit G1311 68705 111 Seal Wash Option Kit G
36. educes the pressure pulsation to values below 1 of system pressure that will be sufficient for most applications and for all gradient analyses For applications using sensitive detectors the compressibility settings can be optimized by using the values for the various solvents described in Table 8 on page 56 If the solvent in use is not listed in the compressibility tables when using isocratic mixtures of solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings When using mixtures of solvents it is not possible to calculate the compressibility of the mixture by interpolating the compressibility values of the pure solvents used in that mixture or by applying any other calculation In these cases the following empirical procedure has to be applied to optimize your compressibility setting 1 Start the pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test via Lab Monitoring and Diagonistic Software LMD 3 Your pump must be connected to a control software e g ChemStation EZChrom OL etc or handheld controller with which the pressure and ripple can be monitored otherwhise connect a signal cable between the pressure output of the pump and a recording device for example 339X integrator and set parameters
37. ersonal injuries e g shock hazard when the cover is opened and the module is connected to power gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed 18 1200 Series Isocratic Pump User Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear of the module The male end of each power cord is different and designed to match the wall socket of a particular country or region The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit Electric Shock gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury
38. fication please see Overview of Main Assemblies on page 100 Please reportmissing or damaged parts to your local Agilent Technologies sales and service office Table5 Isocratic Pump Checklist Description Quantity Isocratic pump 1 Solvent cabinet 1 5065 9981 Amber solvent bottle 1 9301 1450 Bottle head assembly 1 G1311 60003 Waste tube purge valve 1 5042 2461 reorder number 5 m Power cable 1 CAN cable 1 m 1 Remote cable As ordered Signal cable As ordered 1200 Series Isocratic Pump User Manual Table5 Isocratic Pump Checklist Installing the Pump 3 Description Quantity Service Manual Accessory kit see Table 6 on page 27 1 1 Accessory Kit Contents G1311 68705 Table6 Accessory Kit Contents G1311 68705 Description Part Number Capillary pump to injection device length 900 mm ID 0 17 mm Seal insert tool Wrench 1 4 5 16 inch Wrench 14 mm Hex key 4mm Corrugated Waste Tube 1 2 m Corrugated Waste tube reorder number 5 m Velocity regulator reorder number pack of 3 PTFE Frit G1329 87300 01018 23702 8710 0510 8710 1924 8710 2392 no PN 5062 2463 5062 2486 01018 22707 1200 Series Isocratic Pump User Manual 27 3 Installing the Pump otimizing the Stack Config Optimizing the Stack Configuration If your isocratic pump is part of a complete Agilent 1200 Series system you can ensure optimum performance by limiting the confi
39. filled with solvent to prolong the lifetime of the wash seal Always use a mixture of bidistilled water 90 and isopropanol 10 as wash solvent This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water For information on the installation of the active seal wash option refer to Installing the Seal Wash Option on page 88 1200 Series Isocratic Pump User Manual 53 5 Optimiziming Performance When to Use Alternative Seals 54 The standard seal for the pump can be used for most applications However applications that use normal phase solvents for example hexane are not suited for the standard seal and require a different seal when used for a longer time in the pump For applications that use normal phase solvents for example hexane we recommend the use of the polyethylene seals part number 0905 1420 pack of 2 These seals have less abrasion compared to the standard seals Polyethylene seals have a limited pressure range 0 200 bar When used above 200 bar their lifetime will be significantly reduced DO NOT apply the seal wear in procedure performed with new standard seals at 400 bar 1200 Series Isocratic Pump User Manual Optimiziming Performance 5 Optimize the Compressibility Compensation Setting The compressibility compensation default setting is 100 x 10 bar for the pump This setting represents an average value Under normal conditions the default setting r
40. first plunger The pressure in the plunger chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume With a compressibility value set the processor calculates a compensation volume that is depending on the backpressure in the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previous described loss of volume during the delivery stroke of the first plunger 1200 Series Isocratic Pump User Manual 11 1 introduction How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each plunger stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes will generate pressure pulsations of smaller amplitude than higher stroke volumes at same flow rates In addition the frequency of the pressure pulsations will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes resulting in less flow ripple will improve composition ripple The module uses a processor controlled spindle system to drive its plungers The normal stroke volume is optimized for
41. guration of the system stack to the following configuration This configuration optimizes the system flow path ensuring minimum delay volume Flow connections in the stack Example setup with 0 17 mm ID green capillaries Solvent bottle pump G1311 60003 bottle head assembly PTFE tubings Solvent Cabinet Pump autosampler G1312 67305 SST green Degasser Pump purge valve waste 5062 2461 PTFE tubing wide bore reorder pack Pump Instant Pilot Autosampler Autosampler column compartment G1313 87305 SST green Column compartment column G1316 87300 SST green Column detector DAD G1315 87311 SST coated VWD 5062 8522 PEEK Column Compartment Detector waste DAD 0890 1713 PTFE wide bore VWD 5062 8535 PEEK 5062 2463 Detector corrugated waste tubing reorder pack Figure 5 Recommended Stack Configuration Front View 28 1200 Series Isocratic Pump User Manual Installing the Pump 3 Optimizing the Stack Configuration NOTE For a detailed view of the flow connections refer to the section Flow Connections in the product information of the individual modules Remote cable 5061 3378 Pressure output to recorder CAN Bus cable to handheld controller AC power CAN Bus cable for inter module communication 5181 1516 0 5 m 5161 1519 1 0 m GPIB or LAN to control software Analog signal to recorder AC power Figure 6 Rec
42. he vacuum tubes Priming the system by using the pump at high flow rate 8 5 ml min is recommended when pumping system was turned off for a length of time for example overnight and if volatile solvent mixtures are used or e solvents have been changed 1200 Series Isocratic Pump User Manual Installing the Pump 3 Priming with a Syringe WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Before using a new degasser or new tubings for the first time 1 Prime all tubings with at least 30 ml of iso propanol no matter whether the channels will be used with organic mobile phase or with water If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows ao oo A Ww Replace the current solvent with adequate organic solvent see table above if current solvent is organic or with water if current solvent is an inorganic buffer or contains salt Disconnect solvent outlet tube from your pump Connect syringe adapter to solvent outlet tube Push syringe adapter onto syringe Pull syringe plunger to dra
43. idity 21 hydraulic system 22 l initialization 9 installation pump module 31 installation power cords 19 instrument status indicator 61 amp 61 internet 124 L Lab Advisor software 58 aboratory bench 20 amp power supply 60 amp status 60 amp instrument status 61 eak test 59 ine frequency 21 ine voltage 21 iter counters counter liter 67 ithium batteries 119 M main assemblies overview 69 main assemblies 100 maintenance procedures 6 7 maintenance procedures 12 maintenance 126 replacing firmware 97 materials in contact with mobile phase 10 missing parts 26 non operating altitude 21 non operating temperature 21 not ready condition 61 0 operating Altitude 21 operating temperature 21 optimum performance 28 outlet ball valve 77 outlet ball valve 108 overview pump 7 P parts active inlet valve 110 damaged 26 main assemblies 100 missing 26 outlet ball valve 108 pump head with seal wash 106 pump head 104 pump housing and main assemblies 103 pump housing and main assemblies 101 pHrange 22 physical specifications 21 piston chamber 7 piston 8 46 86 power supply indicator 60 power supply lamp 60 power consideration 18 power consumption 21 power cords 19 power switch 32 prerun condition 61 pressure sensor readings 35 pressure pulsation 12 22 55 pressure range 54 pressure test 59 pressure operating range 22 priming withapump 40 43 52 with asyringe 40
44. ion time at constant room temperature 1 or 10pl min whatever is greater Operating range 0 40 MPa 0 400 bar 0 5880 psi up to 5 ml min Operating range 0 20 MPa 0 200 bar 0 2950 psi up to 10 ml min lt 2 amplitude typically lt 1 at 1 ml min isopropanol at all pressures gt 10 bar 147 psi User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Agilent Control Software Chemstation EZ Chrom OL etc For pressure monitoring 2 mV bar one output Controller area network CAN GPIB RS 232C APG Remote ready start stop and shut down signals LAN optional Extensive diagnostics error detection and display through control module and Agilent Lab Monitor amp Diagnostic Software leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas 1200 Series Isocratic Pump User Manual Site Requirements and Specifications 2 Table4 Performance Specification Agilent 1200 Series Isocratic Pump GLP features Housing Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable For use with flow rates below 500 l min a vacuum degasser i
45. lded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1200 Series Isocratic Pump User Manual 13 1 Introduction Electrical Connections 14 The GPIB connector is used to connect the pump with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your pump The switches are preset to a default address This address is recognized at powercycling the module The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1200 Series module data transfer and synchronization One analog output provides a pressure signal for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated an
46. ly understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series Isocratic Pump User Manual Contents Contents 1 Introduction 5 Introduction to the Isocratic Pump 6 Overview of the Hydraulic Path 7 Instrument Layout 13 Electrical Connections 14 Agilent 1200 Series Interfaces 16 2 Site Requirements and Specifications 17 Site Requirements 18 Physical Specifications 21 Performance Specifications 22 3 Installing the Pump 25 Unpacking the Isocratic Pump 26 Optimizing the Stack Configuration 28 Installing the Isocratic Pump 31 Connecting Modules and Control Software 34 Flow Connections of the Isocratic Pump 37 Priming and Purging the System 40 4 Using the Isocratic Pump 45 Hints for Successful Use of the Pump 46 Solvent Information 47 Prevent Blocking of Solvent Filters 48 Algae Growth in HPLC Systems 49 5 Optimiziming Performance 51 When to Use a Vacuum Degasser 52 When to use the Seal Wash Option 53 When to Use Alternative Seals 54 Optimize the Compressibility Compensation Setting 55 1200 Series Isocratic Pump User Manual Contents Troubleshooting and Diagnostics 57 Agilent Lab Advisor Software 58 Overview of the Pump s In
47. mbly 1200 Series Isocratic Pump User Manual 109 8 Parts and Materials for Maintenance Active Inlet Valve Assembly Table 17 Active Inlet Valve Assembly Item Description Part Number 1 Active inlet valve body No cartridge included G1312 60025 2 Valve cartridge 400 bar 5062 8562 Figure 31 Active Inlet Valve Assembly 110 1200 Series Isocratic Pump User Manual Parts and Materials for Maintenance 8 Accessory Kit G1311 68705 Table 18 Tools and Accessories Description Part Number Wrench 14 mm Seal insert too PTFE Frit pack of 5 Corrugated waste tube 1 2 m Corrugated waste tube reorder number 5 m Velocity regulator reorder number pack of 3 Hex key 4mm Wrench 1 4 5 16 inch Capillary pump to injection device length 900 mm ID 0 17 mm 8710 1924 01018 23702 01018 22707 no PN 5062 2463 5062 2486 8710 2392 8710 0510 G1329 87300 1200 Series Isocratic Pump User Manual 111 8 Parts and Materials for Maintenance Seal Wash Option Kit G1311 68711 Table 19 Active Seal Wash Option kit for module Description Part Number Seal wash pump assembly includes pump casette and pump motor 5065 9953 Pump casette Silicone 5042 8507 Support ring seal wash 2 EA 5062 2465 Secondary seal pre installed in support rings 0905 1175 Gasket wash seal 2 EA for re order pack of 6 5062 2484 Seal keeper 2 EA 5001 3743 Silicone rubber tubing 1mm I D 3 m 0890 1764 Seal pack
48. n the isocratic pump is used with concentrated buffer solutions TT oe oer lle Figure 2 Hydraulic Path 1 Introduction How Does the Pump Work The liquid runs from the solvent reservoir to the active inlet valve The pump assembly comprises two substantially identical plunger pump units Both pump units comprise a ball screw drive and a pump head with one sapphire plunger for reciprocating movement in it A servo controlled variable reluctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first plunger to move at twice the speed of the second plunger The solvent enters the pump heads close to the bottom limit and leaves it at its top The outer diameter of the plunger is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first plunger has a stroke volume in the range of 20 100 ul depending on the flow rate The microprocessor controls all flow rates in a range of 1 ul 10 ml min The inlet of the first pumping unit is connected to the active inlet valve which is processor controlled opened or closed allowing solvent to be drawn into the first plunger pump unit The outlet of the first plunger pump unit is connected through the outlet ball valve and the damping unit to the inlet of the se
49. n the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are ful
50. ny signs of leaks Always exchange the purge valve frit too when exchanging the seals When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions of 0 1 Molar or higher will be used for long time periods Check the pump plungers for scratches when changing the plunger seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal After changing plunger seals apply the seal wear in procedure see Exchanging the Pump Seals and Seal Wear in Procedure on page 83 1200 Series Isocratic Pump User Manual Using the Isocratic Pump 4 Solvent Information Always filter solvents through 0 4 um filters small particles can permanently block the capillaries and valves Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric and nitric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt gt 2COCly 2HCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process remove
51. of 2 5063 6589 Seals insert tool 01018 2370 112 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 9 Appendix General Safety Information 114 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 118 Lithium Batteries Information 119 Radio Interference 120 Sound Emission 121 Solvent Information 122 Agilent Technologies on Internet 124 ee Agilent Technologies 113 9 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 114 1200 Series Isocratic Pump User Manual Appendix 9 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a prote
52. ommended Stack Configuration Rear View 1200 Series Isocratic Pump User Manual 29 3 Installing the Pump If a single stack configuration becomes too high e g if an additional module like a G1327A ALS Thermostat is added or if your bench is too high a two stack configuration may be a better setup Separate the stack between pump and autosampler and place the stack containing the pump on the right side of the stack containing the autosampler 30 1200 Series Isocratic Pump User Manual Installing the Pump 3 Installing the Isocratic Pump Parts required Preparations Part number Description 1 Pump 1 Power cord for other cables see text below 1 G4208A Control Software ChemStation EZChrom OL etc 1 G1323B and or a handheld controller Instant Pilot or Control Module Locate bench space Provide power connections Unpack the pump Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspecti
53. on by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1 Place the module on the bench in a horizontal position 1200 Series Isocratic Pump User Manual 31 3 Installing the Pump Installing the Isocratic Pump 2 Ensure the power switch on the front of the module is OFF switch stands out Status lamp Lf i J ci l Power switch z x y Serial number Figure 7 Front of module 3 At the rear of the module move the security lever to its maximum right position 4 Connect the power cable to the power connector at the rear of the module The security lever will prevent that the cover is opened while the power cord is connected to the module 32 1200 Series Isocratic Pump User Manual Installing the Pump 3 Installing the Isocratic Pump 5 Connect the required interface cables to the rear of the isocratic pump see Connecting Modules and Control Software on page 34 Security lever Slot for interface board O cy O Analog pressure 2mv bar APG Remote RS 232C Configuration switch Figure 8 Electrical Connections 6 Connect the capillary solvent tube and waste tubing see Flow Connections of the Isocratic
54. ostic Software Pressure Test The pressure test is a quick test designed to determine the pressure tightness of the system After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight up to 400 bar see Service Manual Leak Test The leak test is a diagnostic test designed to determine the pressure tightness of the pump When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance The following sections describe these functions in detail see Service Manual 1200 Series Isocratic Pump User Manual 59 6 Troubleshooting and Diagnostics Status Indicators Two status indicators are located on the front of the module The lower left one indicates the power supply status the upper right one indicates the module status Status indicator Cc s F Power supply indicator Figure 11 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON When the indicator is off the module is turned OFF Otherwhise check power connections availability of power or check functioning of the power supply 60 1200 Series Isocratic Pump User Manual Troubleshooting and Diagnostics 6 Instrument Status Indicator The instrument status indicator indicates one of four pos
55. ot use an excessively damp cloth that liquid can drip into the module 1200 Series Isocratic Pump User Manual Maintenance 7 Early Maintenance Feedback EMF Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The pump provides a series of EMF counters for the pump head Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The pump provides the following EMF counters e Pump Liquimeter e Pump seal wear Pump Liquimeter The pump liquimeter displays the total volume of solvent pumped by the pump head since the last reset of the counters The pump liquimeter can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Seal Wear Counters The seal wear counters displa
56. plication 46 C cable CAN 26 power 26 remote 26 signal 26 capillary pump to injection device 27 changing solvents 41 cleaning the pump 66 columns 6 compressibility compensation 11 22 55 condensation 20 connections flow 37 control software 35 control software 36 counter seal wear 67 counter EMF 67 D damaged packaging 26 damaged parts 26 delay volume 10 28 delivery checklist 26 Diagnostic software 58 dimensions 21 dual piston in series design 7 E early maintenance feedback EMF 13 electrical connections descriptions of 14 electrostatic discharge ESD 65 96 EMF limits 68 EMF counters 67 environment 18 20 1200 Series Isocratic Pump User Manual error condition 61 error messages 59 ESD electrostatic discharge strap 66 Exchanging the Active Inlet Valve Cartridge 75 exchanging active inlet valve 71 73 internal parts 64 outlet ball valve 77 outlet ball valve 71 pistons 71 86 pump seals 71 purge valve frit 79 purge valve 79 purge valve frit 71 purge valve 71 wash seals 71 88 F features GLP 23 instrument layout 13 safety and maintenace 22 firmware updates 97 upgade downgrade 97 flow cell 122 solvent information 122 flow connections 37 flow precision 22 22 flow range 22 frequency range 21 H hex key set 27 125 Index hexagonal key 4mm 82 83 86 92 hexagonal key 4mm 95 highest injection precision 52 highest precision 46 hints for successful use 46 hum
57. plungers up and down between the two limits During the up movement of the first plunger the solvent in the pump head is pressed through the outlet ball valve into the second pumping unit The second plunger draws in half of the volume displaced by the first plunger and the remaining half volume is directly delivered into the system During the drawing stroke of the first plunger the second plunger delivers the drawn volume into the system Table 1 lsocratic Pump Details Dead volume 800 1100 pl dependent on back pressure Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST gold sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby Adapter SST gold Purge valve SST gold PTFE ceramic PEEK For pump specifications see Performance Specifications on page 22 1200 Series Isocratic Pump User Manual Introduction 1 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the
58. r Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1200 Series Isocratic Pump User Manual 121 9 Appendix Solvent Information 122 Flow Cell To protect optimal functionality of your flow cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add a small of organic solvents e g acetonitrile or methanol 5 Use of Solvents Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CH
59. re 14 Active Inlet Valve Assembly 6 Insert the new valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 1200 Series Isocratic Pump User Manual 73 7 Maintenance 7 Position the valve so that the solvent inlet tube connection points towards the front 8 Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn 9 Reconnect the adapter at the active inlet valve 10 Reconnect the solvent inlet tube to the adapter Reconnect the active inlet valve cable to the connector in the Z panel 11 Reinstall the front cover After an exchange of the valve it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it used to be when the system was still working properly 74 1200 Series Isocratic Pump User Manual Maintenance 7 Simple Repairs Exchanging the Active Inlet Valve Cartridge When If internally leaking backflow Tools required Wrench 14mm Pair of Tweezers Parts required Part number Description 1 G1312 60025 Active inlet valve body 1 5062 8562 Valve cartridge 400 bar Preparations Switch off pump at the main power switch and unplug the power cable Solvent inlet tube Capillary pump to injection device pol Connector Active inlet valve Active inlet valve cable Adapter Figure 15 Exchanging the Active Inlet Valve 1 Remove the front co
60. remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace plunger if scratched AA EIN Plunger surface 86 1200 Series Isocratic Pump User Manual Maintenance Simple Repairs 7 3 Reassemble the Pumphead assembly Pump head 1200 Series Isocratic Pump User Manual 87 7 Maintenance Installing the Seal Wash Option When Tools required Parts required Preparations 88 When installing seal wash option 4 mm hexagonal key Screwdriver Pozidrive 1 Part number Description 1 1 G1311 68711 Active Seal Wash Option kit Isocratic or Quaternary pump G1312 68711 Active Seal Wash Option kit Binary pump Switch off the pump at the main power switch Remove the front cover Remove the top cover and foam By using a screwdriver remove the metal plug in the z panel AW o Y WWW ay AM NOY PA Figure 20 Removing the metal plug from the z panel Insert the socket delivered with the Seal Wash pump assembly into the hole on the z panel Guide the wire of the active seal wash assembly through the hole and screw it onto the z panel 1200 Series Isocratic Pump User Manual Maintenance 7 4 Guide the wire over the fan and plug the connector onto the mainboard connector P7 Figure 21 Wire connected to the mainboard 5 Replace the foam and top cover 6 Disconnect all capillaries and tubes from the pump head an
61. rface Test ChemStation Instant Pilot Control Module Agilent Lab G4208A G1323B Monitor amp Diagnostic Software Pressure Test Yes Yes Yes Yes Leak Test Yes Yes Yes Yes 62 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 7 Maintenance Introduction to Maintenance and Repair 64 Simple Repairs 64 Exchanging Internal Parts 64 Warnings and Cautions 65 Using the ESD Strap 66 Cleaning the Module 66 Early Maintenance Feedback EMF 67 EMF Counters 67 Using the EMF Counters 68 Overview of Maintenance and Repair 69 Simple Repairs 71 Checking and Cleaning the Solvent Filter 72 Exchanging the Active Inlet Valve 73 Exchanging the Active Inlet Valve Cartridge 75 Exchanging the Outlet Ball Valve 77 Exchanging the Purge Valve Frit or the Purge Valve 79 Removing the Pump Head Assembly 82 Exchanging the Pump Seals and Seal Wear in Procedure 83 Exchanging the Plungers 86 Installing the Seal Wash Option 88 Exchanging the Wash Seals 92 Reinstalling the Pump Head Assembly 95 Exchanging the Optional Interface Board 96 Replacing the Module s Firmware 97 ee Agilent Technologies 63 7 Maintenance Introduction to Maintenance and Repair Simple Repairs The module is designed for easy repair The most frequent repairs such as plunger seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Simple Repai
62. rs on page 71 Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the module from the stack removing the covers and disassembling the module The security lever at the power input socket prevents that the module cover is taken off when line power is still connected 64 1200 Series Isocratic Pump User Manual Maintenance 7 Warnings and Cautions Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvent
63. s are used CAUTION Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 1200 Series Isocratic Pump User Manual 65 7 Maintenance Using the ESD Strap Electronic boards are sensitive to electronic discharge ESD In order to prevent damage always use an ESD strap when handling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 12 Using the ESD Strap Cleaning the Module Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an exessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 66 The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do n
64. s required 1200 Series Isocratic Pump User Manual 23 2 Site Requirements and Specifications Performance Specifications 24 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 3 Installing the Pump Unpacking the Isocratic Pump 26 Damaged Packaging 26 Delivery Checklist 26 Accessory Kit Contents G1311 68705 27 Optimizing the Stack Configuration 28 Installing the Isocratic Pump 31 34 Connecting Modules and Control Software Connecting Agilent 1200 Series modules 34 Connecting an Agilent 1200 Series Vacuum Degasser 35 Connecting control software and or control modules 35 Flow Connections of the Isocratic Pump 37 Priming and Purging the System 40 Priming with a Syringe 41 Priming with the Pump 43 ka a Agilent Technologies 25 3 Installing the Pump Unpacking the Isocratic Pump 26 Damaged Packaging Upon receipt of your module inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the instrument has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carrier s inspection Delivery Checklist Ensure all parts and materials have been delivered with the isocratic pump The delivery checklist is shown in Table 5 on page 26 To aid in parts identi
65. s the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1200 Series Isocratic Pump User Manual 47 4 Using the Isocratic Pump Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae e Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001M sodium azide to the solvent e Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed 48 1200 Series Isocratic Pump User Manual Using the Isocratic Pump 4 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow
66. sal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1200 Series Isocratic Pump User Manual 119 9 Appendix Radio Interference Cables supplied by Agilent Technoligies are screened to provide opitimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 120 1200 Series Isocratic Pump User Manual Appendix 9 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operato
67. sed to be when the system was still working properly 1200 Series Isocratic Pump User Manual Maintenance 7 Exchanging the Outlet Ball Valve When If internally leaking Tools required Wrench 1 4 inch Wrench 14mm Parts required Part number Description 1 G1311 60012 Outlet ball valve Preparations Switch off pump at the main power switch Remove the front cover Before exchanging the outlet ball valve you can try to clean it in a sonic bath for 5 10 minutes Place the valve in an upright position in a small beaker with alcohol 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet ball valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body 3 Check that the new valve is assembled correctly and that the gold seal is present if the gold seal is deformed it should be replaced Valve body la m oe Gold seal aa i J Lil H Plastic cap WY A Figure 16 Outlet Ball Valve Parts 1200 Series Isocratic Pump User Manual 71 7 Maintenance Simple Repairs 4 Reinstall the outlet ball valve and tighten the valve 5 Reconnect the valve capillary Valve capillary Outlet ball valve Figure 17 Exchanging the Outlet Ball Valve 78 1200 Series Isocratic Pump User Manual Maintenance 7 Exchanging the Purge Valve Frit or the Purge Valve When Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across
68. sible instrument conditions When the status indicator is OFF and power switch light is ON the module is in aprerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a setpoint or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis A flashing yellow status indicator indicates that the module is in its resident mode Call your local service provider for assistance upon observing this error condition A flashing red status indicator indicates a severe error during the startup procedure of the module Call your local service provider for assistance upon observing this error condition 1200 Series Isocratic Pump User Manual 61 6 Troubleshooting and Diagnostics User Interfaces Depending on the User Interface the available test vary Some descriptions are only available in the Service Manual Table9 Test Functions available vs User Inte
69. solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description Concentrated nitric acid 35 Bidistilled water 1 Beaker Preparations Remove solvent inlet tube from the adapter at the AIV 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 35 for one hour 2 Thoroughly flush the filter with bidistilled water remove all nitric acid some capillary columns can be damaged by nitric acid 3 Replace the filter 72 1200 Series Isocratic Pump User Manual Maintenance 7 Exchanging the Active Inlet Valve When If internally leaking backflow Tools required Wrench 14mm Pair of Tweezers Parts required Part number Description 1 G1312 60025 Active inlet valve body 1 5062 8562 Valve cartridge 400 bar Preparations Switch off pump at the main power switch and unplug the power cable 1 Remove the front cover 2 Unplug the active inlet valve cable from the connector 3 Disconnect the solvent inlet tube at the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow 4 Unscrew the adapter from the active inlet valve 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head Active inlet valve body Valve cartridge Figu
70. the frit at a flow rate of 5 ml min of H30 with purge valve opened Purge valve if internally leaking Tools required Wrench 1 4 inch Wrench 14mm e Pair of tweezers or toothpick Parts required Part number Description 5 01018 22707 PTFE frit pack of 5 1 G1311 60009 Purge valve Preparations Switch off pump at the main power switch Remove the front cover 1 Using a 1 4 inch wrench disconnect the pump outlet capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it Remove the plastic cap with the gold seal from the purge valve 1200 Series Isocratic Pump User Manual 79 7 Maintenance Simple Repairs 5 Using a pair of tweezers or a toothpick remove the frit Valve body PTFE fit Golds Plastiecap sssttsi i lt lt C Figure 18 Purge Valve Parts 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Reinstall the cap with the gold seal NOTE Before reinstallation always check the gold seal A deformed seal should be exchanged 8 Insert the purge valve into the pump head and locate the pump outlet capillary and the waste tube as shown in Figure 19 on page 81 80 1200 Series Isocratic Pump User Manual Maintenance 7 Simple Repairs 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Purge valve
71. the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume The stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended Early Maintenance Feedback EMF The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to use them see Agilent Lab Advisor 12 1200 Series Isocratic Pump User Manual Introduction 1 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels mo
72. timize the Compressibility Compensation Setting 55 ee Agilent Technologies 51 5 Optimiziming Performance When to Use a Vacuum Degasser 52 The pump does not necessarily require degassing But for the following conditions the vacuum degasser is recommended e if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires highest injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 ml min Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser lines are empty you should prime the vacuum degasser before running an analysis The vacuum degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the degasser with a syringe is recommended when e vacuum degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the vacuum degasser by using the pump at high flow rate 140 x 345 x 435 mm 5 5 x 13 5 x 17 inches is recommended when e pump was turned off for a length of time for example during night and volatile solvent mixtures are used or e solvents have been changed
73. to the inlet adapter of the active inlet valve Fix the tube in the clips of solvent cabinet and isocratic pump Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the pump is not part of a Agilent 1200 Series System stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the pump outlet capillary pump to injection device to the outlet of the purge valve 1200 Series Isocratic Pump User Manual Installing the Pump Flow Connections of the Isocratic Pump 8 Purge your system before first use see Priming and Purging the System on page 40 Bottle head assembly Solvent tube Solvent cabinet Tube clip Purge valve Pump head Inlet adapter Active inlet valve Waste tubing Outlet capillary to autosampler Figure 10 Flow Connections of the Isocratic Pump 1200 Series Isocratic Pump User Manual 3 39 3 Installing the Pump Priming and Purging the System 40 If a degasser is installed it can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the vacuum degasser or system with a syringe is recommended when e vacuum degasser or connected tubings are used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in t
74. ven though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting 1200 Series Isocratic Pump User Manual 115 9 Appendix When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 116 1200 Series Isocratic Pump User Manual Appendix 9 Safety Symbols Table 20 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage
75. ver 2 Unplug the active inlet valve cable from the connector 1200 Series Isocratic Pump User Manual 75 7 76 Maintenance 3 Disconnect the solvent inlet tube at the inlet valve be aware that solvent may leak out of the tube due to hydrostatic flow 4 Unscrew the adapter from the active inlet valve 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly 7 Before inserting the new valve cartridge clean the area in the actuator assembly Flush the cartridge area thoroughly with alcohol 8 Insert a new valve cartridge into the actuator assembly make sure the valve cartridge is fully inserted into the actuator assembly 9 Insert the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 10 Position the valve so that the solvent inlet tube connection points towards the front 11 Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn 12 Reconnect the adapter at the active inlet valve 13 Reconnect the solvent inlet tube to the adapter Reconnect the active inlet valve cable to the connector in the Z panel 14 Reinstall the front cover After an exchange of the valve cartridge it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it u
76. w at least 30 ml of solvent through degasser and tubing Replace the priming solvent with the new solvent of your choice 8 Pull syringe plunger to draw at least 30 ml of solvent through degasser and tubing Disconnect syringe adapter from solvent tube 10 Connect solvent tube to your pump 1200 Series Isocratic Pump User Manual 41 3 42 Installing the Pump When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes with your selected flow rate before starting any application This will allow the vacuum degasser to properly degas the solvent in the degasser tubes The pump should never be used for priming empty tubings never let the pump run dry Use the syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 1200 Series Isocratic Pump User Manual Priming with the Pump Installing the Pump 3 When the pumping system has been turned off for a certain time for example overnight oxygen will rediffuse into the solvent channel between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Therefore priming of the vacuum degasser and the pumping system is required before starting an appli
77. white or yellowish white deposits on filters Typically black particles from the 1200 Series Isocratic Pump User Manual 49 4 50 Using the Isocratic Pump normal wear of the piston seals do not cause the PTFE frits to block over short term usage Please refer to the section Exchanging the Purge Valve Frit or the Purge Valve on page 79 in this manual Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as gradient performance problems intermittent pressure fluctuations etc Algae growth may also be the possible source for failures of the ball valves and other components in the flow path How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle part number 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 1200 Series Isocratic Pump User Manual 1200 Series Isocratic Pump User Manual 5 Optimiziming Performance When to Use a Vacuum Degasser 52 When to use the Seal Wash Option 53 When to Use Alternative Seals 54 Op
78. y a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 1200 Series Isocratic Pump User Manual 67 7 68 Maintenance Using the EMF Counters The user settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of pump components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When performance indicates maintenance is necessary take note of the values displayed by pump liquimeter and seal wear counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1200 Series Isocratic Pump User Manual Maintenance 7 Overview of Maintenance and Repair Overview of Maintenance and Repair Figure 13 on page 69 shows the main assemblies of

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