Temperature Verification System Overview 1
Contents
1. Probe Dummy A1 A2 A12 A11 C4 C3 C9 C10 F4 F3 F9 F10 H1 H2 H12 H11 Temperature Verification Tests for the 9700 4 9 Setpoint Screen After starting the TNU test the heated cover will heat to 37 C and the block will stabilize at the 37 C setpoint TNU Performance Sample temp 25 9 C Cover temp 31 C Cover Setpoint is 35 C Cover must be within 1 C of Setpoint F1 F2 F3 F4 F5 Once the heated cover has stabilized and the block has reached the 37 C setpoint the instrument will request placement of the probe in wells A1 and A2 After starting the test the block will begin cycling up to 94 C and then immediately back to the setpoint for the measurement The instrument pauses 30 seconds to allow the temperature to stabilize A digital thermometer reading is taken and entered using the numeric keypad This process is repeated for the remaining seven sample block wells shown in Figure 4 1 dummy Lu Frant of instrument tr Ll a B e O Q 2 r m e Hj 5 C 5 C O Q OOOQOOOQOQO S16 Sale OC OOC OOOLCQOQO LOL ILC CUL s OOeg O0Oo0e 00 OO OOSO00s OOOOGOGO eoooooo e H Figure 4 1 9700 Sample Block Wells for TNU Measurement 4 10 Temperature Verification Tests for the 9700 Procedure To run the Temperature Non Uniformity Test TNU Step Action 1 From the Diagnostics screen pres2. Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Fl F2 F3 FA F5 Press F2 TNU to display the TNU Setup screen TNU Performance Sample temp 25 0 C Cover temp 105 C Place probe in well A2 dummy in well A3 Press Run F1 F2 F3 F4 F5 This screen instructs you to set up the RTD probe assembly Note Both the probe and the dummy probe are moved to seven different locations in the block during the Temperature Non Uniformity test After installing the probe assembly slide the heated cover forward and pull the lever down Press F1 Run to initiate the Temperature Non Uniformity Test This displays the TNU Setpoint Values screen TNU Performance Sample temp 25 0 C Cover temp 104 C Setpoint is 94 C Cover must be within 1 0 C of setpoint F1 F2 F3 F4 F5 Temperature Verification Tests for the 2400 2 11 To run the Temperature Non Uniformity Test Step Action 5 When the temperature stabilizes at 94 C and the counter decrements to zero read the digital thermometer TNU Performance Sample temp 94 0 C Cover temp 104 C Enter actual block temperature F1 F2 F3 F4 F5 Use the numeric keys to type the temperature displayed on the digital thermometer in the highlighted Enter actual block temperature field Press Enter The sample block and heated cover now approach 37 C TNU Performance Samp
3. When you complete the test move the ON OFF RANGE switch to the OFF position 1 2 Temperature Verification System Overview 1000 PLATINUM RTD 0 00385 42 V MAX Figure 1 Front view of digital thermometer Temperature Verification System Overview 1 3 Replacing the The digital thermometer is powered by a 9V battery which has been installed at the Battery factory If the battery is low the LO BAT indicator will appear on the display Install a new battery immediately if the LO BAT indicator appears To replace the battery Step Action 1 Remove the three 7 16 in long screws securing the back cover of the digital thermometer Figure 2 2 Install the 9 V battery as shown in Figure 2 Make sure the battery terminals are contacting the battery clips 3 Replace the back cover and secure it with the three screws removed in step 1 GR1093 Figure 2 Digital thermometer shown with back cover removed 1 4 Temperature Verification System Overview Temperature Display Differences Using the Probe While Running Programs The sample temperature display on the instrument will be different than the display on the digital thermometer during heating or cooling transitions This is because the digital thermometer measures block temperature while the thermal cycler measures sample temperature The instrument sample temperature display is a function of the tube type and the react
4. F1 F2 E3 FA F5 Use the numeric keys to type the value displayed on the digital thermometer into the highlighted Enter actual block temperature field Press Enter This will initiate the second segment of the temperature verification process The heated cover and sample block now approach the second setpoint temperature 45 C Calibration Verification Block temp 85 C Cover temp 85 C Enter actual block temperature 00 0 Run F1 F2 F3 F4 F5 When the temperature stabilizes at 45 C and the counter decrements to zero read the digital thermometer Use the numeric keys and type the value displayed on the digital thermometer in the highlighted Enter actual block temperature field Press Enter This is the last temperature entry The GeneAmp 9700 displays the digital thermometer measurements in the right hand column of the Calibration Verification screen Calibration Verification Actual temperature at 85 C XX X Actual temperature at 40 C XX X Run F1 F2 F3 F4 F5 11 Press Accept to accept the verification values or Cancel to reject the values Temperature Verification Tests for the 9700 4 7 Post Test and Clean Up Procedures Performing TNU post test procedures and clean up 1 After you have completed all measurements remove the probe assembly from the sample block and turn off the digital thermometer 2 Clean the oil from the sample block using cotton
5. ennnen 1 6 Technical Support oes te dt er ou pee debe ed PIOS HEU RERO UE I res 1 9 Temperature Verification Tests for the 2400 2 1 Temperature Verification Tests llle 2 1 Preparation of the RTD Probe Assembly 20 0 ccc eee eee ne 2 2 Temperature Calibration Verification Test 02 0 0 cee ec eee eee 2 4 Temperature Non Uniformity Test 0 0 00 eee ee eee eee 2 9 Temperature Verification Tests for the 9600 39 1 Temperature Verification Tests 0 0 ec eee eee nes 3 1 Test Results eR ee eA Roa eas gb RERK Se I ERA E T Rey Rede 3 8 The Temperature Uniformity Test 0 0 0 0 ee cee eee I anes 3 10 Temperature Verification Tests for the 9700 41 Temperature Verification Tests llle rhe 4 1 The REID Probe Assembly 2s eR Re RATE der e e Rb ERR RD UR 4 2 The Calibration Verification Tests utsari sais cece e 4 3 The Temperature Non Uniformity Test lese 4 8 Temperature Verification System Overview Introduction Tests Performed Parts Included Use the Temperature Verification System to perform the following tests on your GeneAmp PCR Systems Temperature Calibration Verification Test Temperature Uniformity Test Note Carefully unpack the kit and check the parts included against the following list If any part is damaged or missing contact the shipping carrier and Applied Biosystems immediately Digital T
6. F1 F2 F3 F4 F5 Use the numeric keys to type the value displayed on the digital thermometer into the highlighted Enter actual block temperature field 2 6 Temperature Verification Tests for the 2400 To perform the second segment of the calibration test Step Action 1 Press Enter This will initiate the second segment of the temperature calibration process The heated cover and sample block now approach the second setpoint temperature 56 C Calibration Verification Block temp 56 1 C Cover temp 56 C Enter actual block temperature 00 0 F1 F2 F3 FA F5 2 When the temperature stabilizes at 56 C and the counter decrements to zero read the digital thermometer 3 Use the numeric keys and type the value displayed on the digital thermometer in the highlighted Enter actual block temperature field 4 Press Enter This will initiate the third segment of the temperature calibration process The heated cover and sample block will approach the third setpoint temperature 20 C Calibration Verification Block temp 20 1 C Cover temp 35 C Enter actual block temperature 00 0 F1 F2 F3 F4 F5 5 When the temperature stabilizes at 20 C and the counter decrements to zero read the digital thermometer 6 Use the numeric keys and type the value displayed on the digital thermometer in the highlighted Enter actual block temperature field 7 Press Enter This is the last temperat
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9. and Celera Genomics businesses Printed in the USA 09 2001 Part Number 4303200 Rev B an Applera business
10. display on the digital thermometer may not match the temperature display on the System 9600 This is due to the effect of the heated sample block cover on the RTD probe If you suspect any temperature calibration problems perform the temperature calibration verification test described on pages 4 through 9 of these instructions O no QOOOQOCQOQOCQO N QOOOOQOQOQ e00 e575e0e See eese DIODOS QOOOOQOQOQO e0 0e 09 070e QOOOOQOQOQO ee eeese QOOOOOOO 0000000 GR1089 e0 5 09 7e Post Test and Clean Up Procedures Performing TNU post test procedures and clean up 1 After you have completed all measurements remove the probe assembly from the sample block and turn off the digital thermometer Clean the oil from the sample block using cotton swabs Test Results Calculate the test results as follows Forthe 16 Setpoint 1 measurements 95 C hold subtract the lowest measured temperature from the highest measured temperature For the 16 Setpoint 2 measurements 40 C hold subtract the lowest measured temperature from the highest measured temperature If either result is more than 1 C your instrument must be serviced by an Applied Biosystems Service Representative 3 12 Temperature Verification Tests for the 9600 Temperature Verification Tests for the 9700 Temperature Verification Tests Types of Tests C
11. well A12 Press Run The sample block will now approach 94 C and return to the 37 C setpoint TNU Performance Sample temp 37 0 C Cover temp 38 C Place probe in well A12 dummy in well A11 Press Run Run F1 F2 F3 F4 F5 After the instrument counts down 30 seconds read the digital thermometer Use the numeric keys to type the temperature displayed on the digital thermometer in the highlighted Enter actual block temperature field Press Enter 10 Repeat steps 5 through 10 on wells C4 C9 F4 F9 H1 and H12 Make sure you place the measuring cone of the probe assembly into these wells and the dummy probe into adjacent wells as shown in Figure 4 1 on page 4 10 Note When measuring wells in row H leave a loop of wire over the sample block before securing the tray over the probe assembly 11 After all eight wells have been measured at 37 C the heated cover will warm up to 105 C and the test will repeat measuring TNU at 94 C 12 After the heated cover reaches 105 C you will be instructed to move the probe to well A1 Repeat steps 5 through 10 for the 94 C setpoint for all eight wells Note Before being instructed to move the probe to another well the sample block will cool to 37 C 13 When the GeneAmp 9700 completes the temperature Non Uniformity test it displays the actual values gathered during testing 14 Press Accept or Cancel 15 Turn off the digital ther
12. 222 164 420 2 61 222 168 Denmark Naerum 45 45 58 60 00 45 45 58 60 01 Finland Espoo 358 0 9 251 24 250 358 0 9 251 24 243 France Paris 33 0 1 69 59 85 85 33 0 1 69 59 85 00 Germany Weiterstadt 49 0 6150 101 0 49 0 6150 101 101 Hungary Budapest 36 0 1 270 8398 36 0 1 270 8288 Italy Milano 39 0 39 83891 39 0 39 838 9492 Norway Oslo 47 23 12 06 05 47 23 12 05 75 Poland Lithuania Latvia and Estonia 48 22 866 40 10 48 22 866 40 20 Warszawa Portugal Lisboa 351 0 22 605 33 14 351 0 22 605 33 15 Russia Moskva 7 095 935 8888 7 095 564 8787 South East Europe Zagreb Croatia 385 1 34 91 927 385 1 34 91 840 Spain Tres Cantos 34 0 91 806 1210 34 0 91 806 1206 Sweden Stockholm 46 0 8 619 4400 46 0 8 619 4401 Switzerland Rotkreuz 41 0 41 799 7777 41 0 41 790 0676 The Netherlands Nieuwerkerk a d IJssel 31 0 180 331400 31 0 180 331409 United Kingdom Warrington Cheshire 44 0 1925 825650 44 0 1925 282502 All other countries not listed 44 0 1925 282481 44 0 1925 282509 Warrington UK Japan Japan Hacchobori Chuo Ku Tokyo 81 3 5566 6230 81 3 5566 6507 Latin America Del A Obregon Mexico 305 670 4350 305 670 4349 Temperature Verification System Overview 1 11 To Reach Technical We strongly encourage you to visit our Web si
13. 2400 Performing the Temperature Calibration Verification Test Step To perform the first segment of the calibration test Action 1 From the Diagnostics screen press F3 TmpVer This displays the Temperature Verification screen Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity F1 F2 F3 F4 F5 2 Press F1 Temp This displays the Calibration Setup screen Calibration Verification Block temp 25 C Cover temp 105 C Place probe in well B4 dummy in well B5 Press Run F1 F2 F3 F4 F5 This screen instructs you to set up the RTD probe assembly After installing the probe assembly slide the heated cover forward and pull the lever down Press F1 Run to initiate the Calibration Verification Test This automatically verifies the temperature of the sample block and displays the Setpoint Values screen Calibration Verification Block temp 24 9 C Cover temp 104 C Cover must be within 10 of setpoint F1 F2 F3 F4 F5 Note To exit the test at any time press F5 Cancel Temperature Verification Tests for the 2400 2 5 To perform the first segment of the calibration test continued Step Action 5 When the temperature stabilizes at 92 C and the counter decrements to zero read the digital thermometer Calibration Verification Block temp 92 0 C Cover temp 91 C Enter actual block temperature 00 0
14. 600 3 3 The Temperature Calibration Verification Test To perform the first phase of the calibration test Procedure Step Action 1 Turn on the digital thermometer by moving the ON OFF RANGE switch to the 200 position Turn on the GeneAmp PCR System 9600 The main menu appears Press the OPTION key three times to move the cursor to UTIL then press ENTER The utilities menu appears Press the OPTION key twice to move the cursor to DIAG then press ENTER The following display appears Run the Verify Calibration Diagnostic Test Test 45 by pressing 5 then ENTER Note To ensure maximum accuracy the temperatures of the heated cover and the sample block are the same in this test This prevents the heated cover from affecting the accuracy of the RTD probe The temperature of the sample block and heated cover will go to 40 C and the following display will appear Going to 40 C Cvr xxC Blk xx xC This display shows the current temperature of the block cover Cvr xxC and sample block BIk xx xC When the temperature of the block cover is within ten degrees of the sample block temperature the following display appears Wait 3 minutes Time MM SS B1k 95 0C This display shows the current sample block temperature Blk 40 0C and a clock which counts up from zero in minutes and seconds Time MM SS When the clock reaches three minutes the following display appears Record Tem
15. D and dispose of it in accordance with all local state federal and environmental health regulation and laws Other Please describe how equipment was cleaned or disinfected Attach additional paper if necessary By accepting authorization to return this product assume all responsibility and liability for biological chemical and radiological decontamination and cleaning understand that Applied Biosystems has no obligation to repair or service any product unless adequate information is provided to ensure the safety of all personnel handling the exposed product Print Name Title Company Institution Phone Number Signature Date Fax Number 1 8 Temperature Verification System Overview Technical Support Contacting Technical Support To Contact Technical Support by E Mail Hours for Telephone Technical Support To Contact Technical Support by Telephone or Fax You can contact Applied Biosystems for technical support by telephone or fax by e mail or through the Internet You can order Applied Biosystems user documents MSDSs certificates of analysis and other related documents 24 hours a day In addition you can download documents in PDF format from the Applied Biosystems Web site please see the section To Obtain Documents on Demand following the telephone information below Contact technical support by e mail for help in the following product areas Product Area E mail
16. Sales offices and their telephone and Fax numbers IMPORTANT When shipping your Temperature Verification System ship the meter and probe in the black case provided and enclose a Decontamination Certificate Decontaminating Decontaminate the temperature probe by gently swabbing the probe cone with a The Temperature cotton swab using a 20 Clorox 80 water mixture Observe the following Probe precautions when decontaminating your probe Do not submerge the probe in Clorox Do not disassemble the the probe assembly Do not separate the cones 1 6 Temperature Verification System Overview CERTIFICATE OF DECONTAMINATION Return Authorization 1 This document must be completed in full signed by the customer operator of all Applied Biosystems equipment and handled as follows Attach to all Applied Biosystems products prior to returning to either a Applied Biosystems manufacturing facility Field Office or Service Repair Center for the purpose of repair refurbishing trade in or replacement Provide to all Applied Biosystems Representatives before they perform service on any Applied Biosystems equipment which has been located within a biological or radioactive materials laboratory Instrument model type Instrument serial number Reason for return service RMA or RAN number Decontamination Information and Procedure 1 Has this product been exposed to any infectious agents assigned to biosafety level
17. Thermal Cycler Temperature Verification System For GeneAmp PCR Systems 2400 9600 9700 User s Manual Applied EB Biosystems Copyright 2001 Applied Biosystems All rights reserved For Research Use Only Not for use in diagnostic procedures ABI PRISM and the ABI PRISM design AmpErase Applied Biosystems Aquapore AmpliCover Anitron Biobytes Brownlee FastPhoramidite GeneScan Genotyper HLP INHERIT Masterpiece MicroAmp MicroCoat MPLC NEWGUARD ONESTEP OPC PCR MATE Phosphalink POLYPORE Precipitette ProBlott PROCISE ProFocus ProSort ProSpin SeqEd Sequence Navigator SPHERI5 SPHERI10 StockMarks Stretch Synergy SynthAssist TaqMan and VeloSep are registered trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries ABI AmpF STR AutoAssembler BaseSprinter CATALYST GeneAssist LV40 MatchMaker PDQ Primer Express and ProSorb are trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries AmpliTaq EnviroAmp and GeneAmp are registered trademarks and AmpliTaq Gold is a trademark of Roche Molecular Systems Inc All other trademarks are the sole property of their respective owners Contents Temperature Verification System Overview I I Introduction emere be Peo babe heed be ine eb beads gees 1 1 Using the Digital Thermometer I 1 2 Getting the Temperature Verification System Recalibrated
18. alibration Verification tests the sample block against temperature accuracy specifications Temperature Non Uniformity tests the temperature uniformity in the sample block System Contents Equipment Required The Temperature Verification System is required to perform these tests The Temperature Verification System should include the following 9 9 9 Digital thermometer with 9V battery installed RTD probe Cotton swabs Light mineral oil 9700 probe tray IMPORTANT Refer to the instructions included with your Temperature Verification System for a detailed description of digital thermometer operation Temperature Verification Tests for the 9700 4 1 The RTD Probe Assembly Schematic This schematic shows the probe tray from the top view Note the locations of the probe and dummy and also the notch Dummy Probe in B6 Probe in A6 o0 lo oO oIoSDI oie 10 IOO OIOIOIOIDIOIOIDIDIOIOIO WON 1 ER 000C ne ieke TOIOOIOIOMOIOIOIS SS ia e Set ion eor OE O Notch always faces front The Tray Assembly The 9700 temperature verification system is equipped with a tray assembly that ensures accurate measurement of temperature during the calibration verification and TNU procedures The tray consists of a 96 well plate with caps There are nine locations where no caps are present One location is for the calibration verification test and the other eight locatio
19. ature 40 C Hold Time 2 00 minutes Ramp Time 0 00 minutes Cycles 99 Note Refer to your Users Manual for detailed instructions on how to set up and run a CYCL program 2 On the third cycle measure the temperature of well A1 90 seconds into Setpoint 1 95 C setpoint temperature using the digital thermometer see Figure 7 The time remaining clock on the run time display will read 0 30 30 seconds Record this temperature 3 Still on the third cycle measure the temperature of well A1 90 seconds into Setpoint 2 40 C setpoint temperature using the digital thermometer The time remaining clock on the run time display will read 0 30 30 seconds Record this temperature 95 40 Temp oc Time 4 After you measure the second temperature of well A1 turn the cover knob completely counterclockwise then slide the heated cover back Move the probe assembly to wells A4 and A5 placing the dummy probe in A5 Slide the heated cover forward then turn the cover knob clockwise until the white mark on the knob and the white mark on the cover are aligned Temperature Verification Tests for the 9600 3 11 To perform the TNU test continued 7 Repeat the measurements on wells A4 A8 A12 C1 C4 C8 C12 E1 E4 E8 E12 H1 H4 H8 and H12 Make sure you place the measuring cone of the probe assembly into these wells and the dummy probe into adjacent wells see Figure 8 Note The temperature
20. d cover will go to 95 C and the following display will appear Going to 95 C Cvr xxC Blk xx xC This display shows the current temperature of the block cover Cvr xxC and sample block Blk xx xC When the temperature of the block cover is within ten degrees of the sample block temperature the following display appears Wait 3 minutes Time MM SS B1k 95 0C This displays shows the current sample block temperature BIk 95 0C and a clock which counts up from zero in minutes and seconds TimezMM SS When the clock reaches three minutes the following display appears Record Temperature Time MM SS B1k 95 0C 8 Measure the temperature of well E1 using the digital thermometer Record this temperature as T 95 Temperature Figure 3 1 illustrates temperatures during the Calibration Verifciation Test Measurements 0 3 95 Clock starts Time in minutes Figure 3 1 Temperature measurements in the temperature calibration verification test IMPORTANT To exit the test at any time press the STOP key This will return you to the Review History File display Press 5 and ENTER to return to the Verify Calibration Diagnostic test Temperature Verification Tests for the 9600 3 7 Calculating Test Results Calculating the Test Perform the following procedure to calculate the results of the test You will need to Results refer to the calibration label in your Users Manual for the High and L
21. e If the measured temperature of well E1 was 39 9 C and the Low Offset printed on your calibration label is 0 1 you would make the following calculation Block Average at 40C 39 9 0 1 39 8 C In this example since 39 8 C does not differ by more than 0 75 C of your programmed target temperature your instrument would not need to be recalibrated 3 8 Temperature Verification Tests for the 9600 Completing the Calibration Verification Test Procedure To complete the test Step Action 1 Remove the probe assembly from the sample block and move the digital thermometer ON OFF RANGE switch to the OFF position 2 Clean the oil from wells D1 and E1 using cotton swabs IMPORTANT If your instrument needs to be recalibrated contact an Applied Biosystems Service Representative Temperature Verification Tests for the 9600 3 9 The Temperature Uniformity Test Preparation Note Use this procedure to test the temperature uniformity of the sample block in the GeneAmp PCR System 9600 Equipment Required Temperature Verification System See page 3 1 for items that are part of ths system Preparation for the Temperature To set up the thermal cycler for the TNU test Uniformity Test MUSEOS y Step Action 1 If the the sample block heated cover is in the forward position turn the cover knob completely counterclockwise then slide the cover back 2 Coat all the wells in samp
22. e uniformity in the sample block System Contents Equipment Required The Temperature Verification System is required to perform these tests The Temperature Verification System should include the following 9 9 9 Digital thermometer with 9V battery installed RTD probe Cotton swabs Light mineral oil 2400 probe tray IMPORTANT Refer to the instructions included with your Temperature Verification System for a detailed description of digital thermometer operation Temperature Verification Tests for the 2400 2 1 Preparing the RTD Probe Assembly for Tests Description The RTD Probe Assembly consists of two cones one of which measures the temperature of the sample well The wire is attached to the cone that does not measure the temperature of the sample well This cone is a dummy probe Probe in B4 Dummy Probe in B5 Probe Assembly Thread probe wires through notch Figure 2 1 RTD Probe Assembly Preparation WARNING BURN HAZARD The sample block is hot to the touch and can cause burns To prepare the RTD Probe Assembly for the Calibration Verification Test Step Action 1 If the System 2400 heated cover is in the forward position lift the lever then slide the cover back 2 Using a cotton swab coat wells B4 and B5 with mineral oil 1 2 3 4 5 6 7 8 Front of instrument AIC OO OOOOQO BOOO eeooo OOOO Oo Oo 3 Place the probe tray on the sample block so that the notch faces t
23. erification Test Overview The RTD probe and the digital thermometer are used to take temperature readings of one sample well at three different setpoint temperatures 92 C 56 C 20 C Description of the When the Calibration Verification Test begins the block and heated cover each move Beginning Test toward the first setpoint of 92 C The block temperature will increase and the cover Functions temperature decrease After the heated cover reaches 92 C and the block reaches the temperature of the heated cover 10 C the instrument pauses three minutes to stabilize the temperature WARNING BURN HAZARD The sample block is hot to the touch and can cause burns The Stabilizing at Setpoint screen appears This is the start of the 3 minute stabilization period Calibration Verification Hot Block temp 92 1 C Cover temp 92 C Stabilizing at setpoint 3 00 F1 F2 E3 FA F5 Figure 2 2 Stabilizing at Setpoint Screen When three minutes have elapsed the Setpoint screen will be displayed Figure 2 3 The digital thermometer reading of the sample well temperature is entered into the Enter Actual Temp field This process is repeated at each of the three setpoints Calibration Verification Block temp 92 0 C Cover temp 91 C Enter actual block temperature 00 0 F1 F2 F3 F4 F5 Figure 2 3 Setpoint screen for entry of digital thermometer readings 2 4 Temperature Verification Tests for the
24. ex number when requesting documents following the procedures below by phone for fax a From the U S or Canada call 1 800 487 6809 or delivery from outside the U S and Canada call 1 858 712 0317 b Follow the voice instructions to order the documents you want Note There is a limit of five documents per request through the a Access the Applied Biosystems Technical Support Web site at Internet for fax or http www appliedbiosystems com techsupp e mail delivery b Under Resource Libraries click the type of document you want c Enter or select the requested information in the displayed form then click Search d In the displayed search results select a check box for the method of delivery for each document that matches your criteria then click Deliver Selected Documents Now or click the PDF icon for the document to download it immediately e Fill in the information form if you have not previously done so then click Deliver Selected Documents Now to submit your order Note There is a limit of five documents per request for fax delivery but no limit on the number of documents you can order for e mail delivery 1 12 Temperature Verification System Overview Temperature Verification Tests for the 2400 Temperature Verification Tests Types of Tests Calibration Verification tests the sample block against temperature accuracy specifications Temperature Non Uniformity tests the temperatur
25. he front of the instrument Place the Probe Assembly into wells B4 and B5 so that the dummy probe sits in B5 Thread the probe wire through the notch in the probe tray Connect the probe to the digital thermometer oci amp Slide the heated cover forward and pull the lever down 2 2 Temperature Verification Tests for the 2400 To prepare the RTD Probe Assembly for the Calibration Verification Test Step Action 8 Turn on the digital thermometer by moving the ON OFF RANGE switch to the 200 position To prepare the RTD Probe Assembly for the Temperature Non Uniformity Test Step Action 1 If the System 2400 heated cover is in the forward position lift the lever then slide the cover back 2 Using a cotton swab coat wells A2 and A3 with mineral oil 1 2 3 4 5 6 7 8 Front of instrument AJOOQOOOOOQOQ sOOooeeooo CIO UOI Gy Or 3 Place the probe tray on the sample block so that the notch faces the front of the instrument 4 Place the Probe Assembly into wells A2 and A3 so that the dummy probe sits in A3 5 Thread the probe wire through the notch in the Probe tray Figure 2 1 6 Connect the probe to the digital thermometer 7 Slide the heated cover forward and pull the lever down 8 Turn on the digital thermometer by moving the ON OFF RANGE switch to the 200 position Temperature Verification Tests for the 2400 2 3 Temperature Calibration V
26. hermometer with 9V battery installed RTD Probe Assembly Cotton swabs P N 401 0066 Light Mineral Oil P N 186 2302 Probe Trays 2400 P N N8031076 9600 P N N801 3837 9700 P N N8050305 IMPORTANT Your RTD probe and digital thermometer have been calibrated together at the factory Therefore do not use the probe or digital thermometer with any other probe or digital thermometer they are not interchangeable 9 9 9 Temperature Verification System Overview 1 1 Using the Digital Thermometer Overview The handheld digital thermometer Figure 1 is used with a platinum resistance temperature device RTD probe to verify that your thermal cycler is still within calibration tolerance It is also used to test the uniformity of your instrument s sample block The digital thermometer has a temperature range of 10 to 110 C and is accurate to within 0 3 C IMPORTANT Do not try to recalibrate or perform any service on the digital thermometer The only user serviceable component in the unit is the battery Instructions Observe the following when using the digital thermometer Make sure the RTD probe is connected to the input connector located at the top of the digital thermometer Toturn on the digital thermometer move the on off range switch to the 200 position IMPORTANT Do not use the 630 range The temperature measured by the RTD probe appears on the digital thermometer display in degrees Celsius
27. ion volume We recommend that you use the RTD probe and digital thermometer only for the temperature calibration verification test and the temperature uniformity test which are described in these instructions on page 1 2 If you use the RTD probe and digital thermometer while running programs other than those used in the two tests be aware that the accuracy of the RTD probe will decrease due to the effect of the heated sample block cover The heated cover normally operates at 105 C The effect of the heated cover on the RTD probe decreases as the temperature of the sample block approaches 99 9 C Temperature Verification System Overview 1 5 Getting the Temperature Verification System Recalibrated Overview IMPORTANT Do not try to recalibrate or perform any service on the digital thermometer The only user serviceable component in the unit is the battery We recommend that your Temperature Verification System be recalibrated once a year In the United States f you are in the United States or Canada get your Temperature Verification System and Canada serviced by calling the Applied Biosystems Service Repair Center at 800 831 6844 The Service Repair Center will give you a Return Authorization Number and an address to which you can send your Temperature Verification System Outside the United f you are outside the United States call your local Applied Biosystems Sales Service States and Canada office See page 1 10 for a listing of
28. le block rows A C E and H with mineral oil using a cotton swab 3 Place the probe tray on the sample block with the notch facing the front of the instrument Note The RTD probe assembly consists of two cones one of which measures the temperature of the sample well The wire is attached to the cone that does not measure the temperature of the sample well this cone is a dummy probe 4 Place the probe assembly into wells A1 and A2 so that the dummy probe sits in A2 Carefully thread the probe wire through the notch in the probe tray Make sure the probe is connected to the digital thermometer mn Front of instrument w gt Q ies m Q OOO0O0O000 DIO OOOO VOOOV OJOJOO 00 09 0 0 9 JOOU 008 000 909000 OOOOQCCQCSs OVOLO OOOOOQOOs O O O O O O O O O O O O O O 2 O O 5 Slide the heated cover forward and turn the cover knob clockwise until the white mark on the knob and the white mark on the cover are aligned 6 Turn on the digital thermometer by moving the ON OFF RANGE switch to the 200 position 3 10 Temperature Verification Tests for the 9600 Performing the Temperature Non Uniformity Test To perform the TNU test 1 Turn on the GeneAmp PCR System 9600 and create a two temperature CYCL program with the following parameters Setpoint 1 Temperature 95 C Hold Time 2 00 minutes Ramp Time 0 00 minutes Setpoint 2 Temper
29. le temp 37 0 C Cover temp 44 C Enter actual block temperature F1 F2 F3 F4 F5 Read the digital thermometer Use the numeric keys to type the temperature displayed on the digital thermometer in the highlighted Enter actual block temperature field 10 Press Enter 11 Slide the heated cover back and move the probe assembly to wells A7 and A6 with the dummy probe in A6 as indicated by the screen shown below TNU Performance Sample temp 37 0 C Cover temp 53 C Place probe in well A7 dummy in well A6 Press Run F1 F2 F3 F4 F5 12 Repeat steps 5 through 10 on wells A7 B1 B4 B8 C2 and C7 Make sure you place the measuring cone of the probe assembly into these wells and the dummy probe into adjacent wells 2 12 Temperature Verification Tests for the 2400 To run the Temperature Non Uniformity Test Step Action 13 When the GeneAmp 2400 completes the Temperature Non Uniformity Test it displays the actual values gathered during testing 94 C 37 C Well A2 XX X XX X B8 A7 XX X XX X C2 B1 XX X XX X C7 B4 XX X XX X 14 Press F1 Accept or F5 Cancel 15 Turn off the digital thermometer remove the probe assembly from the sample block and clean the oil from the sample block Temperature The maximum temperature measured in any well at the 94 C setpoint minus the Non Uniformity minimum temperature measured in any well at the 94 C setpoint m
30. mometer remove the probe and tray assembly from the sample block and clean the oil from the sample block 4 12 Temperature Verification Tests for the 9700 Temperature The maximum temperature measured in any well at the 94 C setpoint minus the Non Uniformity minimum temperature measured in any well at the 94 C setpoint must be equal to or Specifications less than 1 0 C This can be expressed as Max Temp94 C Min Temp94 C lt 1 0 C The maximum temperature measured in any well at the 37 C setpoint minus the minimum temperature measured in any well at the 37 C setpoint must be equal to or less than 1 0 C This can be expressed as Max Temp37 C Min Temp37 C lt 1 0 C Temperature Verification Tests for the 9700 4 13 Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free 1 800 345 5224 Fax 1 650 638 5884 Worldwide Sales Offices Applied Biosystems vast distribution and service network composed of highly trained support and applications personnel reaches into 150 countries on six continents For international office locations please call our local office or refer to our web site at www appliedbiosystems com www appliedbiosystems com AS Applied NP Biosystems Applera Corporation is committed to providing the world s leading technology and information for life scientists Applera Corporation consists of the Applied Biosystems
31. ns are for TNU testing IMPORTANT The 9700 tray must be used for accurate calibration and TNU measurements 4 2 Temperature Verification Tests for the 9700 Performing The Calibration Verification Test Overview The RTD probe P N N8051210 and the digital thermometer included in Temperature Verification Kit P N N8010435 are used to take temperature readings of one sample well at two different setpoint temperatures IMPORTANT A properly calibrated 9700 probe and meter must be used in determining calibration verification The 9600 probe now obsolete is not compatible with the 9700 and will give inaccurate temperature readings The 9700 Temperature Verification System probe is compatible with all 0 2 ml GeneAmp PCR thermal cycler models 2400 and 9600 85 C 45 C Temperature Verification Tests for the 9700 4 3 Preparation To prepare the RTD Probe assembly Step Action 1 If the System 9700 heated cover is in the forward position lift the lever then slide the cover back 2 Using a cotton swab coat wells A6 and B6 with mineral oil Front of 10 11 12 instrument A OOOOOOO O O O O O O O OO OO OO OO OO ee 9p 00 0 0 0 0e JUJU O O O O O O O COODCOODOs JOVO O O O O O O O COCOS O O s O e O O G QOOOOOOOOOOO 3 Place the probe assembly into wells A6 and B6 so that the dummy probe sits in B6 4 Place the probe tray over
32. nt 2 ra 94 Temp oc Move the Move the 37 probe to probe to well A7 well B1 Cycle 42 0 2 3 4 5 6 Time minutes Figure 2 4 The Temperature Non Uniformity test Temperature Verification Tests for the 2400 2 9 Description of About 90 seconds after the test begins the Stabilizing at Setpoint screen is displayed Beginning Test Figure 2 5 Functions TNU Performance Sample temp 94 0 C Cover temp 104 C Stabilizing at setpoint 0 30 esser F1 F2 F3 F4 F5 Figure 2 5 The Setpoint Screen The instrument pauses 30 seconds to allow the temperature to stabilize A digital thermometer reading is taken and entered using the numeric keypad About 40 seconds after the digital thermometer reading is entered the heated cover and sample block approach the second setpoint value of 37 C Again the instrument pauses 30 seconds to allow the temperature to stabilize A digital thermometer reading is taken and entered using the numeric keypad This process is repeated in seven different sample block wells 1 2 3 4 5 6 78 ee ee a ee D0OO0 OO O om cjoeooooeo Figure 2 6 Location of wells used in Temperature Non Uniformity test 2 10 Temperature Verification Tests for the 2400 Procedure To run the Temperature Non Uniformity Test Step Action 1 From the Diagnostics screen press F3 TmpVer This displays the Temperature Verification screen
33. ow Offset values Note If you have more than one GeneAmp PCR System 9600 in your laboratory make sure that the serial number on the calibration label matches the serial number on the instrument you are testing Calculating the Use the following formula to calculate the average block temperature at 95 C T 95 Average Block High Offset hold Temperature at the pet ET 95 C Hold Block Average at 95 C T 95 High Offset If the block average is more than 0 75 C above or below 95 C your GeneAmp PCR System 9600 must be recalibrated For example If the measured temperature of well E1 was 95 2 C and the High Offset printed on your calibration label is 0 1 you would make the following calculation Block Average at 95 C 95 2 0 1 95 3 C In this example since 95 3 C does not differ by 0 75 C from your programmed target temperature your instrument would not need to be recalibrated Note The offset is the number of degrees Celsius that the temperature of well E1 differed from the average temperature of the block when the instrument was calibrated at the factory Calculating the Use the following formula to calculate the average block temperature at the 40 C Average Block hold Temperature at the t40 C T 40 L ffset 40 C Hold Block Average at 40 C T 40 Low Offse If the block average is more than 0 75 C above or below 40 C your GeneAmp PCR System 9600 must be recalibrated For exampl
34. perature Time MM SS B1k 95 0C Measure the temperature of well E1 using the digital thermometer Record this temperature as T 40 See Figure 5 on the next page 3 4 Temperature Verification Tests for the 9600 To perform the first phase of the calibration test continued 7 Press ENTER The temperature of the sample block and heated cover will go to 95 C and the following display will appear Going to 95 C Cvr xxC Blk xx xC This display shows the current temperature of the block cover Cvr xxC and sample block Blk xx xC When the temperature of the block cover is within ten degrees of the sample block temperature the following display appears Wait 3 minutes Time MM SS B1k 95 0C This displays shows the current sample block temperature BIk 95 0C and a clock which counts up from zero in minutes and seconds TimezMM SS When the clock reaches three minutes the following display appears Record Temperature Time MM SS Blk 95 0C 8 Measure the temperature of well E1 using the digital thermometer Record this temperature as T 95 Temperature Verification Tests for the 9600 3 5 To perform the second phase of the calibration test Step Action 1 Turn on the digital thermometer by moving the ON OFF RANGE switch to the 200 position Turn on the GeneAmp PCR System 9600 The main menu appears Press the OPTION key three times to move the cursor
35. ple block heated cover is in the forward position turn the knob completely counterclockwise then slide the cover back 2 Coat wells D1 and E1 with mineral oil using a cotton swab The illustration below shows the location of the wells O SO Oae e QOOOQOQOQOO e CACOOCOCOCOCOCOUE CC CEC Se OC SeCesees QOOOQOQOQOQO JI QOOOQOQOQOQO e Ce eee ess QOOOOQOQOQO QOOOQOQOQOO s GR1088 QOO0O 9000 r 3 Place the probe tray on the sample block so that the probe tray notch faces the front of the instrument Note The RTD probe assembly consists of two cones one of which measures the temperature of the sample well The wire is attached to the cone that does not measure the temperature of the sample well this cone is a dummy probe 3 2 Temperature Verification Tests for the 9600 To prepare the RTD Probe Assembly for the Calibration Verification Test continued Step Action 4 Place the probe assembly into wells D1 and E1 so that the dummy probe sits in D1 Carefully thread the probe wire through the notch in the probe tray Make sure the probe is connected to the digital thermometer e e e P SP LE eo ec eo El Se et et e S eoe e ex 5 Slide the heated cover forward then turn the cover knob clockwise until the white mark on the knob is aligned with the white mark on the cover Temperature Verification Tests for the 9
36. r actual block temperature XXX Temperature Verification Tests for the 9700 4 5 Procedure To run the Calibration Verification Test Step Action 1 From the Diagnostics screen press TmpVer This displays the Temperature Verification screen Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU F1 F2 F3 F4 F5 Press Temp This displays the Calibration Verification Setup screen Calibration Verification Block temp 25 C Place Probe in well A6 dummy in well B6 Press Run Run Note This screen instructs you to set up the RTD probe assembly After installing the probe assembly slide the heated cover forward and pull the lever down Press Run to initiate the Calibration Verification Test This automatically verifies the temperature of the sample block and displays the Setpoint Values screen Calibration Verification Hot Block temp 85 0 C Cover temp 93 C Stabilizing at setpoint 1 37 F1 F2 F3 F4 F5 Note To exit the test at any time press Cancel 4 6 Temperature Verification Tests for the 9700 To run the Calibration Verification Test continued Step Action 5 When the temperature stabilizes at 85 C and the counter decrements to zero read the digital thermometer Calibration Verification Block temp 85 C Cover temp 85 C Enter actual block temperature _ 00 0 Run
37. s 2 3 0r 4 If so please indicate agent and biosafety hazard class 2 Has this product been exposed to toxic carcinogenic or radioactive substances If so please indicate types and quantities used 3 Indicate how the equipment was cleaned or disinfected by checking one or more of the decontamination procedures described below Contact with infectious agents The equipment was thoroughly decontaminated by spraying it or wiping it down with a 1 10 dilution of Clorox brand bleach or equivalent with water The disinfectant remained on the unit for a minimum of 10 minutes before flushing with water Temperature Verification System Overview 1 7 Contact with radioactive material The equipment was thoroughly decontaminated by spraying it or wiping it down with a commercially available decontaminate i e Radiacwash or equivalent The instrument must be surveyed with a Geiger meter and swipe tested with a scintillation counter Results must be attached to this form Chemical contamination All chemical bottles have been removed from the instrument The equipment was thoroughly decontaminated by rinsing areas associated with chemicals with a solvent such as alcohol or water Chemical contamination to valve blocks The valve block was flushed and dried with appropriate gas argon helium or nitrogen In the event that a valve block cannot be flushed properly label it as a HAZARDOUS WASTE SOLI
38. s TmpVer This displays the Temperature Verification screen Temperature Verification Temp Calibration Verification TNU Temperature Non Uniformity Temp TNU F1 F2 F3 F4 F5 2 Press TNU to display the TNU Setup screen TNU Performance Sample temp 25 0 C Cover temp 37 C Place probe in well Al dummy in well A2 Press Run Run This screen instructs you to set up the RTD probe assembly For a detailed procedure see page 4 4 Note Both the probe and the dummy probe are moved to 8 different locations in the block during the Temperature Non Uniformity test 3 After installing the probe assembly slide the heated cover forward and pull the lever down 4 Press Run to initiate the Temperature Non Uniformity Test This displays the TNU Setpoint Values screen TNU Performance Sample temp 25 0 C Cover temp 37 C Setpoint is 37 C Cover must be within 1 0 C of setpoint Temperature Verification Tests for the 9700 4 11 To run the Temperature Non Uniformity Test TNU continued Step Action 5 When the temperature stabilizes at 37 C and the counter decrements to zero read the digital thermometer TNU Performance Sample temp 37 C Cover temp 37 C Enter actual block temperature 00 0 Run F1 F2 F3 F4 F5 Use the numeric keys to type the temperature displayed on the digital thermometer in the highlighted Enter actual block temperature field Press Enter Place the probe in
39. swabs Calibration The calibration verification procedure is fully automated After entering the calibration Verification verification values the instrument will display a pass or fail result Specification 4 8 Temperature Verification Tests for the 9700 The Temperature Non Uniformity Test Overview The temperature Non Uniformity test uses the RTD probe assembly and a digital thermometer to test the temperature uniformity of eight different wells in the sample block IMPORTANT Perform this test after the instrument has been off for at least two hours The first pass of the TNU test will be performed with the heated cover at 37 C If the heated cover is hot when the test is started it will take a considerable amount of time for the heated cover to cool off and the test to start The first eight TNU measurements will be made at 37 C with the heated cover at or near 37 C After completion of the 37 measurements the heated cover will reach and maintain a setpoint of 105 C Once the setpoint is reached the instrument will instruct you to measure TNU for the eight wells at 94 C IMPORTANT firmware version 1 63 or greater If an instrument has an older version of firmware then the The TNU procedure outlined here can only be performed on instruments with 9700 must be upgraded for the TNU procedure to work properly to upgrade the firmware ona 9700 see page 3 18 of the User s Manual or 2 13 of the Service Manual
40. te for answers to frequently asked Support Through questions and for more information about our products You can also order technical the Internet documents or an index of available documents and have them faxed or e mailed to you through our site The Applied Biosystems Web site address is http www appliedbiosystems com techsupp To submit technical questions from North America or Europe Step Action 1 Access the Applied Biosystems Technical Support Web site 2 Under the Troubleshooting heading click Support Request Forms then select the relevant support region for the product area of interest 3 Enter the requested information and your question in the displayed form then click Ask Us RIGHT NOW blue button with yellow text 4 Enter the required information in the next form if you have not already done so then click Ask Us RIGHT NOW You will receive an e mail reply to your question from one of our technical experts within 24 to 48 hours To Obtain Free 24 hour access to Applied Biosystems technical documents including MSDSs Documents on is available by fax or e mail or by download from our Web site Demand To order documents Then by index number a Access the Applied Biosystems Technical Support Web site at http www appliedbiosystems com techsupp b Click the Index link for the document type you want then find the document you want and record the index number c Use the ind
41. the probe assembly with the notch in the tray facing the front of the instrument 5 Thread the probe wire through the notch and carefully seat the probe tray over the probe and wire being sure not to crimp the wire in any wells 6 Connect the probe to the digital thermometer 7 Slide the heated cover forward and pull the lever down 8 Turn on the digital thermometer by moving the ON Off Range switch to the 200 position Description of When the Calibration Verification Test begins the block and heated cover each move Beginning Test toward the first setpoint of 85 C The block temperature will increase and the cover Functions temperature decrease After the heated cover reaches 85 C and the block reaches the temperature of the heated cover 10 C the instrument pauses three minutes to stabilize the temperature WARNING BURN HAZARD The sample block is hot to the touch and can cause burns The Stabilizing at Setpoint screen will be displayed 4 4 Temperature Verification Tests for the 9700 Calibration Verification Block temp 85 1 C Cover temp 85 C Setpoint is 85 C Cover must be within 10 C of setpoint When three minutes have elapsed the Setpoint screen will be displayed The digital thermometer reading of the sample well temperature is entered into the Enter Actual Temp field This process is repeated at each of the two setpoints Calibration Verification Block temp xx x C Cover temp XXX C Ente
42. thesis 1 800 899 5858 then press 15 1 508 383 7855 FMAT 8100 HTS System and Cytofluor 4000 Fluorescence Plate Reader 1 800 899 5858 then press 16 1 508 383 7855 Chemiluminescence Tropix 1 800 542 2369 U S only or 1 781 271 0045 1 781 275 8581 Applied Biosystems MDS Sciex 1 800 952 4716 1 650 638 6223 Outside North America Region Telephone Dial Fax Dial Africa and the Middle East Africa English Speaking and West Asia Fairlands South Africa 27 11 478 0411 27 11 478 0349 South Africa Johannesburg 27 11 478 0411 27 11 478 0349 Middle Eastern Countries and North Africa Monza Italia 39 0 39 8389 481 39 0 39 8389 493 1 10 Temperature Verification System Overview Telephone Fax Region Dial Dial Eastern Asia China Oceania Australia Scoresby Victoria 61 3 9730 8600 61 3 9730 8799 China Beijing 86 10 64106608 86 10 64106617 Hong Kong 852 2756 6928 852 2756 6968 Korea Seoul 82 2 593 6470 6471 82 2 593 6472 Malaysia Petaling Jaya 60 3 758 8268 60 3 754 9043 Singapore 65 896 2168 65 896 2147 Taiwan Taipei Hsien 886 2 22358 2838 886 2 2358 2839 Thailand Bangkok 66 2 719 6405 66 2 319 9788 Europe Austria Wien 43 0 1 867 35 75 0 43 0 1 867 35 75 11 Belgium 32 0 2 712 5555 32 0 2 712 5516 Czech Republic and Slovakia Praha 420 2 61
43. to UTIL then press ENTER The utilities menu appears Press the OPTION key twice to move the cursor to DIAG then press ENTER The following display appears Run the Verify Calibration Diagnostic Test Test 5 by pressing 5 then ENTER Note To ensure maximum accuracy the temperatures of the heated cover and the sample block are the same in this test This prevents the heated cover from affecting the accuracy of the RTD probe The temperature of the sample block and heated cover will go to 40 C and the following display will appear Going to 40 C Cvr xxC Blk xx xC This display shows the current temperature of the block cover Cvr xxC and sample block Blk xx xC When the temperature of the block cover is within ten degrees of the sample block temperature the following display appears Wait 3 minutes Time MM SS B1lk 95 0C This display shows the current sample block temperature BIk 40 0C and a clock which counts up from zero in minutes and seconds Time MM SS When the clock reaches three minutes the following display appears Record Temperature Time MM SS B1k 95 0C Measure the temperature of well E1 using the digital thermometer Record this temperature as T 40 See Figure 5 on the next page 3 6 Temperature Verification Tests for the 9600 To perform the second phase of the calibration test continued 7 Press ENTER The temperature of the sample block and heate
44. ure entry The GeneAmp 2400 displays the digital thermometer measurements in the right hand column of the Calibration Verification screen Calibration Verification Actual temperature at 92 C XX X Actual temperature at 56 C XX X Actual temperature at 20 C XX X F1 F2 F3 FA F5 8 Press F1 Accept to accept the calibration or F5 Cancel to cancel the calibration 9 Turn off the digital thermometer remove the probe assembly and clean the oil from the sample block Temperature Verification Tests for the 2400 2 7 Calibration The digital thermometer reading at each setpoint must be 0 30 C of the instrument Verification setpoint temperature epecicadon Values that are out of specification cannot be saved by pressing Fl Accept 2 8 Temperature Verification Tests for the 2400 Temperature Non Uniformity Test Overview The Temperature Non Uniformity test uses the RTD probe assembly and a digital thermometer to test the temperature uniformity of seven different wells see the list below and Figure 2 4 in the sample block A2 A7 Bi B4 B8 C2 C7 These wells are tested at two different setpoint temperatures 94 C 37 C Schematic A schematic representation of the Temperature Non Uniformity Test is shown below Measure well A2 Measure well A2 Measure well A7 Measure well A7 120 seconds into 70 seconds at 120 seconds at 70 seconds at Setpoint 1 Setpoint 2 Setpoint 1 Setpoi
45. ust be equal to or Specifications less than 1 0 C This can be expressed as Max Temp 94 C Min Temp 94 C lt 1 0 C The maximum temperature measured in any well at the 37 C setpoint minus the minimum temperature measured in any well at the 37 C setpoint must be equal to or less than 1 0 C This can be expressed as Max Temp 37 C Min Temp 37 C lt 1 0 C Temperature Verification Tests for the 2400 2 13 Temperature Verification Tests for the 9600 Temperature Verification Tests Types of Tests Calibration Verification tests the sample block against temperature accuracy specifications Temperature Non Uniformity tests the temperature uniformity in the sample block System Contents Equipment Required The Temperature Verification System is required to perform these tests The Temperature Verification System should includethe following 9 9 9 Digital thermometer with 9V battery installed RTD probe Cotton swabs Light mineral oil 2400 probe tray IMPORTANT Refer to the instructions included with your Temperature Verification System for a detailed description of digital thermometer operation Temperature Verification Tests for the 9600 3 1 Preparation of the WARNING BURN HAZARD The sample block is hot to the touch and can cause RTD Probe burns Assembly To prepare the RTD Probe Assembly for the Calibration Verification Test Step Action 1 If the the sam
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