DabOut LVS v1_2 - Triangle Biomedical Sciences
Contents
1. 3014 CROASDAILE DRIVE DURHAM NORTH CAROLINA 27705 2507 USA gt 919 384 9393 gt FAX 919 384 9595 E MAIL tbs trianglebiomedical com IBS TRIANGLE BIOMEDICAL SCIENCES INC DAB Out LVS Catalog DO LVS 110 Installation Operation amp Service Manual Version 1 2 April 2001 www trianglebiomedical com INNOVATIONS FOR SCIENCE PAGE Insert A 1 AOU TABLE OF CONTENTS TOPIC Warranty Card Introduction General Warnings Regulatory Compliance Trademarks Safety Precautions Instructions Installation Assembly Operation Cartridge Replacement amp Storage Cartridge Disposal Service Trouble Shooting Technical Support Hours of Operation amp Contact Phone Numbers Specifications Description and Measurements Parts List Diagram Parts List Warranty Information Use amp Regulations Disclaimer Limitations and Liability INTRODUCTION Congratulations on your purchase of a new TBS DabOut Large Volume System for removing 3 3 Diaminobenzidine DAB from automated immunohistochemistry IHC stainer waste This innovative instrument is designed to meet the requirements of today s immunohistochemistry laboratory by offering the convenience of complete adaptability to every automated IHC machine The unique system design requires no water hookup ventilation or special electrical plugs The complex matrix inside the replaceable DabOut LVS filters will remove virtually all DAB to levels below delectability Once2. teretigen DO NOT wear clothes that are loose and prone to snagging or catching on metal edges Access to the instrument s internal components is reserved to only those specialists trained in the service of the instrument Always disconnect the unit from the power source before performing maintenance or accessing the electronics Check the end of the intake line occasionally as particle buildup could interfere with the flow volume Avoid probing the cartridge valve with sharp objects as they may puncture the seal aqueous liquid begins to leak from cartridge or instrument from areas other than the line ends turn off unit and disconnect for power source Contact TBS technical service Unused DabOut LVS cartridges DO NOT contain material that require disposal as hazardous waste under United States Resource Conservation and Recovery Act RCRA Flow Rate The DabOut LVS system is designed to process aqueous waste from automatic IHC staining solutions containing DAB The cartridges should be replaced after processing 40 gallons of aqueous DAB waste or 90 days after initial use Waste can be processed at a rate of approximately 1 5 gallons per hour INSTRUCTIONS Installation 1 Remove equipment from shipping carton carefully DO NOT lift the pump using the pump head Save shipping carton and all packing material until proper operation of equipment is confirmed Immediately notify carrier if there is any visible damage to the cart
3. BS s option LIMITATIONS amp LIABILITY In no event will TBS be liable for any indirect special incidental punitive or consequential damage of any kind sustained from any cause relating to the DabOut LVS product In no event shall TBS be liable to buyer in an amount exceeding the purchase price These limitations apply to any claim whether based upon contract negligence strict tort liability or any other legal theory arising out of or relating to the DabOut LVS product Specification and technical data are current at the time publication however they are subject to change without notification Please contact our North Carolina office for updated information 11
4. ality and safety standards and have been found to be in compliance with the following regulatory specifications Applicable Directives 73 23 EEC 93 68 EEC Conforms to EN 61010 1 1993 A2 1995 Safety requirements for electrical equipment for measurement control and laboratory use UL per 460 D Certified to IEC 664 IP21 per IEC 529 TRADEMARKS TBS is a registered trademark and Innovations for Science DabOut and DabOut LVS are trademarks of Triangle Biomedical Sciences Inc Durham NC U S A SAFETY PRECAUCATIONS DabOut LVS is only recommended for use under the following conditions Indoor use only Operation at altitude below 2000m External ambient temperatures between 20 C and 40 C Humidity 80 for temperatures up to 31 C decreasing linearly to 50 at 40 C UL Installation Category II UL Pollution Degree 2 severe visible damage occurs during shipping DO NOT connect the instrument to 8 power source Contact TBS technical service Damage may result if unit is connected to power supply different from that stated in this manual and stated on the identification tag on the bottom of the stand DO NOT use instrument unless it is connected to a properly grounded outlet DO NOT run volatile flammable or corrosive substances through DabOut System Use specific precautions when handling Dab Waste as it may cause irritations CAUTION 3 3 Diaminobenzidine DAB is a suspected carcinogen mutigen
5. be w in line valve and 1 DabOut LVS cartridge DO LVS C Electrical Requirements 110 115VAC 60 HZ 2 0 Amps Dimensions WxDxH in 15 x 15 cm 38 x 38 x 38 Weight Ibs 14 kg 6 4 PARTS LIST Included in the shipment of the DabOut LVS system is the following Quantity Description DabOut LVS cartridge Disposal sealing plug Disposal sealing cap Quick connect tubing adapters Usage log Cartridge support stand Timer 60 rom pump Lengths of yellow Tygon tubing Dip tube and connector valve barb Disposal bag Red warning label 0 Antibacterial ampoules Operators Manual DabOut MSDS Bactericide MSDS S SS eS 4 4 S PA 4 Quick Connects DabOut LVS Support Stand Quick Connects M f gt 40 WARRANTY INFORMATION USE AND REGULATION DabOut is a product for concentration of hazardous waste While DabOut LVS reduces the volume of waste use of DabOut LVS does not exempt the user from laws and regulations applicable to the treatment handling or disposal of such wastes It is the user s responsibility to handle and dispose of the DabOut LVS cartridge and any filtered liquid in compliance with all applicable laws and regulations By using DabOut LVS the user represents and recognizes that the user is knowledgeable in the handling of hazardous wastes and related material assumes the risk of handling such wastes and materials and that the user needs to and will take appropriate
6. ck all lines Check to make sure that the pump is pulling the waste through the cartridge There could be a leak in the hose Replace tubing and connectors If the cartridge is pumping out bubbles The cartridge may be defective Check all of the quick connects to be sure they are fully and properly attached The system has stopped running Check to see timer is on Check to see system is plugged in Use a regular screwdriver to turn pump head If nothing happens call TBS service There is dirty material coming out of cartridge e f color is brown like DAB waste Replace cartridge e f color is light brown it is bacteria Add bactericide If color persists replace cartridge e f color is black it is the cartridge matrix Stop system Run cartridge dry for 20 minutes Then continue filtering If black material persists replace cartridge TECHNICAL SUPPORT TBS Customer Service department can be reached by calling 919 384 9393 Hours of Operation are Monday through Thursday 8am 6pm EST Friday 8am 5pm EST TBS Technical Product Service department can be reached by calling 1 800 871 5975 Hours of Operation are Monday through Friday 7 30am 5pm EST SPECIFICATIONS Catalog Number DO LVS 110 Description DabOut Diaminobenzidine removal system for automated IHC waste Large Volume System LVS includes 2 quick connector adapters disposable sealing cap amp plug stand 60 rpm peristaltic pump timer dip tu
7. convenient after generating the waste Do not allow the waste to sit for long periods of time as bacteria will grow and limit the effectiveness of the cartridge oR OW CAUTION Do not allow Liquid Cover Slip LCS to run through the cartridge Contamination with LCS will severely diminish the binding capacity and flow rate of the cartridge Replacing amp Storage of the Cartridge The DabOut LVS cartridge should be replaced after processing 40 gallons of aqueous DAB waste or 90 days after initial use To store the cartridge between uses disconnect the quick connect adapters from the IN and OUT ports of the cartridge For optimal cartridge performance keep the cartridge hydrated The cartridge should be rehydrated if the pump has been left running after the waste has been drawn completely through the IN tubing To re hydrate the cartridge follow these steps 1 Pour one to two 1 2 liters of distilled water into the waste storage container 2 Draw the distilled water into the cartridge 3 Just as the last of the distilled water is drawn through the tubing and into the cartridge turn off the pump CAUTION There may be a small amount of residual liquid lt 5mls in the cavity of the female portion of the quick connect adapter attached to the OUT port of the cartridge When disconnecting the adapter from the cartridge take care to use a disposable absorbent material as a blotter for any liquid that may still b
8. e present When disconnecting the tubing from the cartridge ALWAYS wear appropriate safety equipment including but not limited to gloves safety glasses and a lab coat 4 As you disconnect the quick connect adapter from the OUT port of the cartridge elevate it to drain excess processed liquid from the tubing into the collection vessel Preparing the Cartridge for Disposal WARNING The cartridge must have all excess liquid removed prior to disposal as a dry solid hazardous waste Simply run the system dry for an hour to remove all excess liquid CAUTION There may be a small amount of residual liquid lt 5mls in the cavity of the female portion quick connect adapter attached to the To Pump port of the cartridge When disconnecting the adapter from the cartridge use a disposable absorbent material as a blotter for any liquid that may still be present When disconnecting the tubing from the cartridge ALWAYS wear appropriate safety equipment including but not limited to gloves safety glasses and a lab coat Drain dispose any liquid that has accumulated in the collection vessel Disconnect the quick connect adapter from the IN port of the cartridge Put the disposal black cap over the OUT adapter on the cartridge While pressing down on the release button insert the blue disposal plug into the IN adapter on the cartridge The cartridge is now ready to be disposed as dry solid hazardous waste Place the cartr
9. idge into the disposal bag zip the bag closed and dispose as chemical hazardous waste for incineration in an approved incinerator with scrubbers DO NOT dispose as red bag infectious waste Pons Oa SERVICE Before using this instrument please make certain that you carefully read this manual Pay particular attention to the precautions Trouble Shooting Only trained service technicians should open the unit WARNING Main voltages are accessible when the housing is removed the unit should be turned off and unplugged before the housing is opened The cartridge has a slow flow rate If the cartridge has filtered close to the 40 gallon limit the flow rate may slow down The typical flow rate is 30 45 minutes per gallon If Liquid Cover Slip LCS has contaminated the cartridge it will slow down the flow rate Replace cartridge If waste stream has a high concentration of BSA it will slow down the flow rate Dilute waste stream with water If the waste stream has a high concentration of detergent surfactants it will slow down the flow rate Dilute waste stream with water Excessive bacteria build up will slow flow rate Use bactericide If the flow of the cartridge is sparatic Check the line for a blockage Check all lines The cartridge may need to be rehydrated The waste container may be empty The system is pumping out air Check to see that the system pump and timer are on Check the line for a blockage Che
10. independent precautions and will comply with all the applicable federal state and local regulations for the handling of DabOut LVS and hazardous material used in connection with DabOut DabOut LVS will not function properly if the enclosed instructions are not followed Safe use of DabOut LVS requires but is not limited to proper assembly of the DabOut LVS system as demonstrated in the enclosed instructions and using appropriate protective devices when using DabOut LVS Users of DabOut LVS should contact their institution s safety officer or safety committee to confirm procedures for using DabOut LVS under the institution s safety procedures DISCLAIMER DabOut LVS is warranted to the buyer to be free of defects in materials and workmanship when used in accordance with the enclosed instructions for a period of one year after delivery TBS will repair replace at it s option any products that are found to be defective provided that the buyer gives written notice of the defect within the warranty period Buyer shall make product available for TBS s inspection or at TBS s option shall return the product to TBS TBS will return a new product to buyer freight prepaid to any location This warranty is in lieu of all other warrantees or obligations expressed or implied TBS expressly disclaims all implied warranties of merchantability or fitness for a particular purpose Buyers exclusive remedy for breach of warranty is replacement or refund at T
11. on or contents Failure to do so may limit your ability to recover damages 2 Connect the power cable to the unit and into a properly grounded outlet Check the label on the rear of the instrument for the electrical requirements DO NOT connect the unit to an outlet that does not match the electrical requirement listed on the label Assembly 1 Set up the system so that the liquid flows from the waste storage container into the IN port of the cartridge through the cartridge then through the peristaltic pump and into the collection vessel see product picture Set up 1 Please refer to the product picture at the back of this manual 2 Locate the DabOut LVS cartridge 3 Place the cartridge in the support stand 4 Attach one length of 3 8 D peristaltic pump tubing long enough to reach your waste storage container to the barbed male quick connect adapter labeled IN 5 Attach another length of 3 8 I D peristaltic pump tubing to the barbed female quick connect adapter This length of tubing should be long enough to pass from the OUT port through the peristaltic pump and to the collection vessel see product diagram 6 Attach the quick connect adapters to the ports located on each side of the top of the cartridge 7 Attach dip tube valve barb to end of IN line tubing 8 At least once every 5 gallons pour one ampoule of antibacterial agent into the waste storage container before running waste into
12. the DAB waste is filtered the effluent can be safely drain disposed The cartridge containing DAB are safe for incineration or can be disposed as a dry solid hazardous waste The completely contained system reduces possible exposure to DAB waste and is engineered to reduce spill potential By providing a hands off method of treating DAB waste at the point of generation DabOut LVS reduces time spent in handling waste and helps contribute to safer lab conditions Reducing the amount of waste that may potentially find it s way into the waterway DabOut LVS saves the environment With routine care this instrument will provide many years of service GENERAL WARNINGS Before using this instrument please make certain that you carefully read this manual Pay particular attention to the precautions and warnings for safe use and operation of this instrument The DabOut LVS Cat DO LVS 110 is an instrument designed for laboratory use It can only be used to filter DAB from automated IHC waste This instrument should only be operated by qualified technicians The warranty applies only if the instrument is used in the correct manner and in accordance with the information and advice provided herein The manufacturer declines all responsibility for possible damages to persons and or objects due to improper use of this instrument REGULATORY COMPLIANCE The pump and timer have been tested by a registered independent body to rigorous international qu
13. the container Then process waste as usual NOTE The tubing used with peristaltic pumps supplied with the DabOut LVS should be changed after 1000 hours of use or after processing 1500 gallons Operation 1 Securely connect the input tubing to the waste storage container If you have Ventana waste containing Liquid Cover Slip LCS use one the three options below to prevent contamination and turn off the pump prior to any LCS running through the system e Use the supplied dip tube to siphon liquid for the bottom of the container e Connect the tubing to a carboy spigot and turn off the pump prior to any LCS running through the system e Decant off the aqueous fraction of the waste and run the tubing from the new aqueous only waste container 2 Label the cartridge with the red warning label Contains Hazardous Waste DAB Diaminobenzidine Securely connect the output tubing to the processed waste collection vessel Turn the timer on The timer controls the peristaltic pump When the waste storage container and the tubing from the waste storage container are empty turn off the pump 6 If you plan to reuse the cartridge refer to section Replacing amp Storing of the Cartridge When the cartridge is ready for disposal refer to section Preparing the Cartridge for Disposal 7 On the usage log supplied with the system mark the number of gallons processed through DabOut LVS 8 Process the waste as soon as
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