SIM Direct Inlet Probe Agilent 5975 MSD Agilent 7000 Triple
Contents
1. E IM Hi E Scientific Instruments Manufacturer GmbH e D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de RESULTS DIP Temperature Accuracy Test Measured Value C Setpoint C Typical Range il Verified By D of Operation Pass Fail erified By Date 5 2 Leak test This procedure consists of the Tune evalutation results MS ChemStation or the Air Water Check MassHunter software described in section 2 10 DIP in Ready status with the push rod outside the MS ion source and the results of an Air Water check while the push rod is located in the ion source The leak test of DIP MS System is passed when the results of both measurements do not essentially differ REQUIREMENTS an autotune and tune evalutation test for 5975 MSD or Air And Water Check for 7000 Triple Q have just been performed CONDITIONS MS Ready DIP Ready Bereit PROCEDURE 1 Start the DIP Software and load the method a_w_check dip 2 Start the DIP start button in the software or controller The push rod will be inserted into the ion source heated up to the source temperature 230 C and be kept there for 10 minutes 3 When the setpoint is reached and the reading stabilizes perform a tune evaluation or an air water check 5975 MSD go to Instrument Edit MS Tune Parmeters and select Air water check 7000 Triple Q MS MS go to Manual Tune Vacuum Control and2. SIME FB 508 0 3 2 Agilent Technologies L Channel Partner Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gm SERVICE MANUAL SIM Direct Inlet Probe for Agilent 5975 MSD and Agilent 7000 Triple Quadrupole Firmware V 1 10 d d i l j Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de Document History Edition 7075e 10 2010 from Firmware Software Version V1 10 and dip v 1 2 The information contained in this document is subject to change without notice SIM GmbH makes no warranty of any kind with regard to this material including but not limited to the implied warranties of merchantability and fitness for a particular purpose SIM GmbH shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material Reproduction adaptation or translation without prior written permission is prohibited except as allowed under the copyright laws Copyright 2010 SIM GmbH Printed in Germany All rights reserved IM i _ Scientific Instruments Manufacturer GmbH e D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de CONTENT 1 PARAMETER MENU OF THE CONTROLLER FIRMWA
3. enables the heating of the push rod outside the ion source Due to the risks of bodily injury and damaging the instrument this mode is reserved to qualified service engineers Temperature Calibration Select ValMode at the DIP Controller see above and DIP Manual 5 6 Put the temperature sensor of a calibrated thermometer indicates the actual value into the push rod tip and isolate these both Select the parameter menu of the controller Press lt Enter gt and keep it pressed while pressing lt arrow up gt lt arrow down gt lt arrow up gt lt arrow down gt then let off the lt Enter gt key so that the diagnostic menu appears press lt Arrow right gt and lt Enter gt so that the cursor in the parameter menu appears the first two numbers represent the parameter number the selected number is taken with lt Enter gt then the belonging value to this parameter will be shown and can be changed Select parameter 00 and enter the password 1000 now all parameter values may be changed Select parameter 01 and calibrate the controller to room temperature example actual value thermometer 20 C controller value 22 C change the value to 20 and press lt Enter gt algebraic sign because the controller value is higher than the actual value otherwise do not use a sign Load the DIP method check_temp dip and start it The tip of the push rod is successively heated to 30 150 250 3
4. 5 2 RE 17 5 3 Coupling test CombiPAL with MSD DIP and GC optional for DIP 45 type with ER EE 18 5 4 DIP ED et Eine E 19 5 5 DIP PCI Performance test for DIP 5975 MSD Systems a aaiaaiaaaaanaasaasaasaasaanaa 22 ENEE eebe 24 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIM Iam 1 Parameter Menu of the Controller Firmware V1 10 Parameter Assignment arme max vate dtu o mreen nr mmm Temp adjustment 0 1 C e foma o a e freeman mmm a to o fn f soo ao as Jemen o soo faa i f aoo T eo s o2 w position inside the DIP 4 90 position ion source 1min 100 default 6 s 8 continuous operation 16 smoothing position of light barrier for tube test D 160 O ring change position 400 o 115 of temperature 32 early start tilt angle adjustment pe Option bits 1 useExStart 2 ready 0x8000 Ox7fft 64 CO cooling dwell time in valve after opening to the oo O 97 activated 1 deactivated Additional remarks to parameter 14 You can combine the items by adding the numbers e g 23 1 2 4 16 for DIP without autosampler or 31 1 2 4 8 16 for DIP with autosampler 95 1 2 4 8 16 64 for DIP with autosampler and CO2 cooling Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIME This menu is reserved to qualified service e
5. directed down to the bottom see blue arrow in the right figure 6 Carefully take the front panel still connected to the DIP system and select the service mode Valve see DIP user manual 5 4 7 Without the push rod still in the vertical position select valve free with the subseauent clicks on the arrow keys right and up and enter for confirmation 44 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de 8 Press the arrow keys to open the valve control it by watching the marker of the valve plug which is directed forwards to the observer when the valve is open see blue arrow in figure on the left 9 Take the dummy push rod for the alignment procedure While the Original push rod is already in the vertical position insert the dummy into the valve as far as possible If this is consistent with the position in the figure left positioning of the push rod in the ion source is correct 4 o_o and you can skip the steps 11 12 transportation safety screw 10 Otherwise please loose the screws of the brass block slightly Now you can cautiously move the valve block until you are able to insert the push rod completely 11 Fix the screws to adjust the correct position of the push rod 12 Remove the dummy and exchange the brass disc with the Teflon disc 13 Click the push rod down while the valve is still open marker for
6. select Air and Water check 4 Compare this result with the tune evaluation report 5975 MSD air and water check 7000 Triple Q MS MS when the push rod was in the Ready position outside the MS ion source see this manual section 2 10 and complete the table in the Results section S47 E IM Hi E Scientific Instruments Manufacturer GmbH e D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de RESULTS LEAK TEST Air Water Air Water Item Check Check Limit for deviation 5 eal Verified By Date push rod outside push rod inside percentage points ion source ion source Ratio 18 69 Abundance Ratio 28 69 5 3 Coupling test CombiPAL with MSD DIP and GC optional for DIP 45 type with 5975 MSD This procedure checks the function of the CombiPAL autosampler by alternating injections into the DIP system and the GC REQUIREMENTS Two additional DIP positions have been defined in the CycleComposer software according to the DIP manual Cycle Composer Verify that the methods GC Injection and DIP Injection the macros Injection DIP and Injection GC and the sequence Injection _GC DIP see Cycle Composer on the disc enclosed are copied into Programs PAL Cycle Composer Combi PAL MSD ChemStation Verify that the folders Injection GC and Injection_DIP see MSChemstation on the disc enclosed are copied into the methods section of t
7. 00000 140000 1500000 130000 1400000 120000 110000 26928 1 Y7 537 255269283797 339 381395409 437451465479403 0 521 53 592 P l T r rr r J e e m T EEN r 7 rrr D t t T ih r HT f r BAMBU sat Time gt 1 00 2 00 3 40 4 00 5 00 6 00 7 00 8 00 9 00 10 00 11 00 12 00 13 00 miz gt 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 50000 112 171 239 369 um 185 199 213 247 297 311325 353 401 424 446 466 481 501 536 o T MBA AT trr trr aed T rrer Ay ae T k miz gt 100 120 200 220 T V T TTT T T TT T UMMA T 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 21 ES IM if Scientific Instruments Manufacturer GmbH E D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de 5 5 DIP PCI Performance test for DIP 5975 MSD Systems This test uses a traceable standard solution to determine the correct performance of the DIP Perform this procedure on both types of probe tips for liquid and for solid samples REQUIREMENTS An autotune has been performed Anew method for DIP Cl measurements has been created in the MS ChemStation software Follow the instructions of the MSD ChemStation manual and note that there are some different inputs to be made e In the menu item Inlet Injection Types select for Sample Inlet Other None and for Injection Source
8. 1 M resp Dip EI Scan M 4 Print the method and verify that method and conditions are the same 5 Load method check_El dip for the DIP system via DIP software see DIP manual 90 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIME The following steps have to be performed with the push rod tip for liquid samples A and the tip for solid samples B 1 After loading the DIP method click the Start button green arrow in the Instrument Control view of the MS software and specify all sample information in the Sample Information panel 2 Click on Start Run and wait for the Ready signal of the MS Acquisition Waiting for remote start 3 Ifthe Ready signal appears in the status display green field you can inject the sample according to the DIP manual see 6 3 by pressing the START key to move the push rod into the Load position 4 Start the DIP using the controller or the DIP software see DIP manual 6 2 Click on the START key of the controller or in the DIP programm to start the measurement RESULTS El PERFORMANCE Main signals Typical values Abundance TIC tnax Typical value i counts counts Tripalmitin e 239 313 551 gt 600 000 Example for DIP 5975MSD System Aaonlaure TIC JAS_BENELUX_001 Didata mns Abundance Scan 916 5 428 min JAS_ BENELUX_002 Didata ms dng A 249 1 6
9. 50 C Please note each temperature of the controller display at the end of the dwell time Calculate the deviation percentage of each temperature and average over all temperatures for example 8 14 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIME 8 Select parameter 11 in the parameter menu and enter the calculated value in the following manner 80 and lt Enter gt for the above mentioned example if the value of the controller is higher than the actual value 80 and lt Enter gt for the above mentioned example if the value of the controller is lower than the actual value 9 Press lt Arrow right gt to return to the diagnostic menu and leave it with lt Enter gt 10 Change the validation mode ValMode to the normal measuring mode Operate in the General Configuration Menu see above and Manual 5 6 15 E IM H Ta Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de 5 DIP Test Procedures Evaluates the DIP tip temperature using a probe oie EES Ves with a calibrated thermometer Air Water Check during DIP operation Evaluates the DIP for leaks Evaluates the function of the DIP 45 Version and Coupling Test CombiPal with MSD DIP the GC together with the EA Evaluates the performance of the MSD El DIP DIP El Performance Test using a traceable standard E
10. External Device and enter the value for Total Runtime take the value displayed in the DIP software minus 1 5 see DIP manual CONDITIONS DIP method check_Cl dip 30 C for 0 1 min 30 350 C at 1 0 s 350 C for 5 min MSD MSD aux temp Aquisition mode MV Mass range high 550 0 Threshold Inst Sampling Mass range low C MS quad temp 150 C MS ion source temp 250 C 292 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIMI PROCEDURE Set up the Methods Load method DIP_Cl_Q1 M for the 5975 MSD MS ChemStation Select PCICH4 U using Instrument Select MS Tune File Save the method to 1 METHODS DIP_Cl_Q1 M Print the method and verify that method and conditions are the same Load method check_Cl dip for the DIP system via DIP software see DIP manual E get Ee The following steps have to be performed with the push rod tip for liquid samples A and the tip for solid samples B 6 After loading the DIP method click the Start button green arrow in the Instrument Control view of the MS Software and specify all sample information in the Sample Information panel 7 Click on Start Run and wait for the Ready signal of the MSD Acquisition Waiting for remote start 8 Ifthe Ready signal appears in the status display green field you can inject the sample according to the DIP manual see 6 3 by pressi
11. Perform MS Autotune from the instrument menu The software will perform an autotune and print out the report 240 5 IM 1 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de When the autotune has completed save it as ATUNE U and then select Tune 2 10 2 2 11 Evaluation from the Qualify menu The software will evaluate the last autotune and print a System Verification Tune report Verify that there is no air and water in the system If there are peaks at 18 28 and 32 amu there may be an air leak in the system Autotune 7000 TripleQ MS MS with DIP Verify that the system has been pumping down for at least 2 4 hours and that the DIP controller is turned on The DIP push rod status is Ready In the Instrument Control view select MS Tune from the instrument menu and select the items print autotune report and save tune file when done The software will perform an autotune save it as atunes el tune xml default file name and print out the report Go to Manual Tune Vacuum Control and select Air And Water Check Print the screen to document the result Verify that there is no air and water in the system If there are peaks at 18 28 and 32 amu there may be an air leak in the system Controller Update of the firmware Switch on the controller Open the program CHIPTOOL download link htt
12. RE V1 10 4 2 DIP INSTALLATION PROCEDURDE aina aaaaaaaaaaaaaaaaaaaaaaaaaaauaaaauaaaauaaaauaaaauaaan 5 2 1 Replacing the 100 Ee ie 5 2 2 Installing the DIP on the Agilent 5975 MSD ainannaaaaaaaaaaaa aaasaaanaanaaanaanaaanannaaananaaaaaa 5 2 3 Installing the DIP on the Agilent 7000 TopleO 7 2 4 Connecting the DIP and he 8 2 5 Configuration of the DIP Controller aiaaaaaaaaaaaaaaannaaanaaanaaanuaanuaaanaaanaaanaaanaaanaaanaa 9 2 6 installing the DIP e 9 2 7 Insert the ChemStation MassHunter methods for the DIR 10 2 7 1 5975 MSD GCHEMSITANON ne e EE 10 2 7 2 7000 Triple Q MS MS MassHunter methods aaxiaaaaaaaaannaanaaaaanuaannuaananaaan 10 2 8 Adjustment of the CombiPAL to the MS DIP System optional 2 22 naa vnaannai 10 2 9 PUSH rod performance EE 10 2 10 Tuning of the DIP MS EE eege 10 2101 Autotune 5975 MOD ee 10 2 10 2 Autotune 7000 TripleQ MS MS with DIR 11 2 11 Controller Update of the firmware a aaaaaaaaaaaaaaaasaaaaaaaanaaanaaanaaanaaanaaanaaanaaanaaaaaa 11 3 EXACT POSITIONING OF THE PUSH ROD IN THE ION SOURCE 12 4 TEMPERATURE CALIBRATION OF THE CONTROLLER anni anina nana nana anna nin 14 4 1 Menu General Configuration aainaaaaaaanaaanaaanaaanaannaannnannuaannnnnnannnnnannannanannaanaai 14 4 2 Temperature Calibration ME 14 5 DIP te as BD E 16 5 1 DIP Temperature accuracy Test 16
13. en this download is done the update is finished Reboot the controller for correct working El Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIMI 3 Exact Positioning of the Push Rod in the lon Source This chapter is valid for all DIP systems for the Agilent 7000 Triple Q and for those for the 5975 MSD starting from serial number SN1938 December 2009 because these systems are not equipped with an inspection glass any longer In case of maintenance work at the push rod or for installing purposes it can be necessary to reposition the push rod in the ion source Then use the following procedure Note Vent the MS before starting the following procedure i e turbopump is off and ion source temperature lt 100 C Turn on the foreline pump to get the prevacuum 2 Remove the housing of the push rod and the front panel see DIP user manual 7 2 but do not disconnect the two cables to the front panel because you have to move the push rod with the display keys later on 3 Fix the transportation safety screw completely shown in the figure next to step no 11 see arrow 4 Move the push rod out of the parking position in the valve by pressing the arrow key up front panel once Manually bring the push rod into the vertical 90 position 5 Remove the Teflon disc left figure and replace it by a brass one right figure The marker of the valve plug should be
14. he MSD ChemStation under Programme MSDChem 1 Methods Moreover copy the sequence Injection GC DIP into the sequence section of the MSD ChemStation CONDITIONS MSD Ready MSD DIP Ready Bereit CombiPAL Power on PROCEDURE 1 Position an empty vial on position 1 at tray 1 of the CombiPAL 2 Open the MSD ChemStation and load the sequence Injection GC DIP possibly check it with edit sequence Open the Cyle Composer with Macro Editor and load the sample list Injection GC DIP Open the DIP software and download the method drive dp Switch to the MSD ChemStation window and start the sequence run sequence Switch to the Cycle Composer window and start the sample list start Watch the alternating injections into the DIP and the GC 3 times each and complete the table below de E EE The sequence has to run completely with the needle in the correct positions 18 Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIM Iam 5 4 DIP El Performance test This test uses a Standard solution with two components to determine the correct performance of the DIP Perform this procedure on both types of probe tips for liquid and for solid samples REQUIREMENTS An autotune has been performed Anew method for DIP El measurements has been created in the MS ChemStation software Follow the instructi
15. ng the START key to move the push rod into the Load position 9 Start the DIP using the controller or the DIP software see DIP manual 6 2 Click on the START key of the controller or in the DIP program to start the measurement RESULTS Cl PERFORMANCE tmax TIC Main signals Typical values Abundance TIC tma Typical value min m z m z counts Example for DIP 5975MSD System 1e 08 1 2e 07 D Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de SIMI APPENDIX DIP Methods drive dip 30 C for 0 1 min 30 35 C at 0 5 s 35 C for 0 05 min heat dip 30 C for 0 1 min 30 400 C at 2 0 s 400 C for 1 min a_w_check dip 30 C for 0 1 min 30 230 C at 1 0 s 230 C for 15 min check_temp dip 30 C for 2 min 30 150 C at 1 0 s 150 C for 2 min 150 250 C at 1 0 s 250 C for 2 min 250 350 C at 1 0 s 350 C for 2 min Check EL dp 30 C for 0 1 min 30 350 C at 0 5 s 350 C for 5 min check_Cl dip 30 C for 0 1 min 30 350 C at 1 0 s 350 C for 5 min 24
16. ngineers only and needs a password Select the parameter menu of the controller in the following way 1 Press lt Enter gt and keep it pressed while pressing lt arrow up gt lt arrow down gt lt arrow up gt lt arrow down gt then let off the lt Enter gt key so that the diagnostic menu appears press lt arrow right gt and lt Enter gt so that the cursor in the parameter menu appears the first two numbers represent the parameter number the selected number is taken with lt Enter gt then the belonging value to this parameter will be shown and can be changed 2 Select parameter 00 and enter the password 1000 3 Change the values of the designated parameter 2 DIP Installation Procedure 2 1 Replacing the ion source Please follow the instructions given in the corresponding Agilent Manual to replace the existing ion source with the provided modified ion source El or Cl 2 2 Installing the DIP on the Agilent 5975 MSD Before installing the DIP System to the Agilent 5975 MSD you have to uninstall the MSD front panel 1 After you have disconnected the required cables remove the MSD front panel carefully Please follow the instructions given in the 5975 MSD manual 2 Demount the two boards for the MSD display see marking on the left and put the MSD front panel aside and Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de 3 Remove the DIP fro
17. nt panel of the DIP System following the instructions given in the DIP manual chapter 7 2 4 Take the DIP front panel and mount the display boards of the MSD onto the preset positions see left 5 Before mounting the DIP system remove the glass plate in front of the MSD ion source compartment 6 To mount the DIP system in front of the MSD first deinstall the valve unit by loosing the two screws see marked circles on the left Now the adapter plate is separated and the four screws are well accessible for the next step 7 Take the adapter plate and mount it in front of the ion source compartment using the four screws see marked circles on the left the screws behind the motors are not visible on this photo 8 Then retighten the valve unit of the DIP system on the adapter plate with the two screws according to the photo of step no 6 9 Replace the original knurled knob of the MSD valve with the provided knob for the DIP System see marked rectangle in the photo left Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de S M jll 10 Mount the push rod cover by reversing the instructions in chapter 7 2 of the DIP manual Make sure that all cables are correctly connected 2 3 installing the DIP on the Agilent 7000 Triple Q Before installing the DIP System to the Agilent 7000 Triple Q MS MS you have to uninstall the MSD front panel After you ha
18. of the DIP manual Make sure that all cables are correctly connected 2 4 Connecting the DIP and the GC Please follow the instructions of the Agilent Manual but pay attention to the difference in installing the DIP controller as you can see in the figures below DIP controller rear panel view On Off Cooling out Power cord receptacle Cooling in APG for Start Remote Cable without System Cable or for Remotemanager with autosampler LAN Cable RS 232 Cable IM i _ Scientific Instruments Manufacturer GmbH e D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de connecting diagram GC MS DIP Controller GC MS DIP Controller Diode Cable Remotemanager Start Remote Cable B06135973 RM 0002 68 90 B 0301 40 02 2 5 Configuration of the DIP Controller The IP adress is assigned in the menu Network configuration of the DIP controller see MSD DIP Manual 5 5 Network configuration 1 Turn on the DIP controller 2 Select the menu Network configuration and scroll with the arrow up and arrow down key in the parameters 3 Set the parameter DHCP Mode to Fixed IP Select the parameter IP adress with the Enter key and change the numbers with the arrow keys 5 Skip back to the basic display by pressing the arrow right key two times Or the IP adress is assigned automatically if
19. ons of the MSD ChemStation manual and note that there are some different inputs to be made e In the menu item Inlet Injection Types select for Sample Inlet Other None for Injection Source External Device for Scan Type MS1 Scan and enter the value for Total Runtime which is displayed in the DIP software DIP method check_El dip see DIP manual Optional CombiPAL is installed correctly on the GC MSD DIP system see DIP manual CONDITIONS for DIP 5975 MSD DIP Ready Bereit DIP method check_El dip 80 C for 0 1 min 30 350 C at 0 5 s 350 C for 5 min wen Ready Mass range low Optional CombiPAL ae PAL method DIP injection PME triglyceride standard Push rod tip A for liquid samples B for solid samples 19 P Scientific Instruments Manufacturer GmbH a D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de CONDITIONS for DIP 7000 Triple Q MS MS DIP Ready Bereit DIP method check_El_scan dip 40 C for 1 min 40 350 C at 1 0 s 350 C for 5 GG C El egen C2FSJJTT qQA triglyceride standard Push rod tip A for liquid samples B for solid samples O O PROCEDURE Set up the Methods 1 Load method DIP_EI Q1 M for the 5975 MSD MS ChemStation or DIP_El_ Scan M for the 7000 Triple Q MS MS 2 Select the actual autotune using Instrument Select MS Tune File ATUNE U or atunes ei tune xml 3 Save the method to 1 METHODS as DIP EI Q
20. p www beck ipc com bc download index asp sp en that will find all installed chips with their ID number Select the chip of the SIM DIP controller and click at the right mouse button Now you can select the FTP server and enter the following items and finish this procedure with the Connect button User ftp Password ftp Representation Type Binary Image Now you can see the contents of the chip at one side of the screen Drive A and the contents of one of the other drives at the other side of the screen Select the drive with the update files of the new firmware version Copy the update files _t exe for SIM controller and or MSDDIP hex for display controller into the A directory of the chip and close the FTP server If you have updated the MSDDIP hex file you have to contiunue with the following procedure 6 7 Select the SIM DIP controller and click at the right mouse button Now you can choose Telnet as connection type Enter the following items and finish the procedure with the Connect button User tel Password tel Automatically login In this TELNET session break _t exe with the shortcut Ctrl Z 9 Type dir so that the directory of A is displayed 11 Type d and Enter into the next command line A gt d so that program D exe is started D exe runs the download of MSDDIP hex from the DIP controller into the display controller Wh
21. p EI Scan M and possibly DIP_Cl _Scan M into the methods section of the MassHunter software under MassHunter GCMS 1 Methods optional with autosampler Copy the macros cycles DIP Liquid Inj cyx and DIP Solid Inj cyx into the PAL section under MassHunter GCMS 1 PALDATA Adjustment of the CombiPAL to the MS DIP System optional Define two new DIP positions for a precise co operation of PAL and DIP see DIP PAL Manual 6 4 3 Create a macro and a new method in the Cycle Composer Software of the autosampler see DIP Manual 6 4 4 and 6 4 5 or copy the files of the folder Cycle Composer into C Programs PAL Cycle Composer Combi PAL Push rod performance test Select the method drive dp in the DIP Software and click Ok Check the correct insertion of the push rod into the MSD ion source and the operational sequence backwards in real and even at the status display of the software Select the method heat dip in the DIP Software and click OK The push rod is inserted into the ion source and heated up to 400 C This temperature is maintained for 1 minute Check the correct temperature run via the status display Tuning of the DIP MS System Autotune 5975 MSD with DIP Verify that the system has been pumping down for at least 2 4 hours and that the DIP controller is turned on The DIP push rod status is Ready In the Instrument Control view select
22. the modus DHCP is selected see MSD DIP Manual 5 5 1 Turn on the DIP controller 2 Select the menu Network configuration and scroll with the arrow up and arrow down key in the parameters 3 Set the parameter DHCP Mode to DHCP see 4 Skip back to the basic display by pressing the arrow right key two times 2 6 Installing the DIP Software Note You can find all files with software and default methods on the disc enclosed Copy the files DIP exe dip enu dip deu into the new folder DIP Copy the default methods in the folder DIP Methods Open the DIP Software Click on File and open the menu Config on eS oe I F Select the DIP controller with a click on the IP adress 2 7 IM if Scientific Instruments Manufacturer GmbH E D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de Insert the ChemStation MassHunter methods for the DIP Note You can find all the folders with the default methods on the disc enclosed 2 7 1 1 2 7 2 2 8 2 9 2 10 2 10 1 5975 MSD ChemStation methods Copy the folders DIP_El_Q1 and DIP_Cl_Q1 into the methods section of the MSD ChemStation under Programme MSDChem 1 Methods optional with autosampler Copy the folders Injection GC and Injection_ DIP into the methods section of the MSD ChemStation 7000 Triple Q MS MS MassHunter methods Copy the folders Di
23. valuates the performance of the MSD PCI using PCICH4 U Test if applicable a traceable calibration standard DIP PCI Performance Test Evaluates the performance of the MSD PCI DIP if applicable using a traceable standard 5 1 DIP Temperature accuracy test This procedure uses a traceable digital thermometer to determine the temperature accuracy of the push rod tip at the DIP system CONDITIONS MS Ready DIP Ready Bereit PROCEDURE 1 With the push rod outside select the Validation Mode of the DIP controller Go to the menu General Configuration see DIP manual 5 6 Menu General Configuration and change the operate modus to the validation modus ValMode with the arrow keys up or down 2 Put the temperature sensor of the calibrated digital thermometer into the tip of the push rod 3 Isolate the tip of the push rod together with the thermometer and shut the cap of the DIP 4 Inthe DIP software select the method check_temp dip and start the temperature program 5 When the first setpoint is reached observe the calibrated thermometer s display until the reading stabilizes 6 Record the thermometer s reading at the end of the 5 minute s dwell time in the table of the Results section Repeat no 5 and 6 for the second and third setpoint In the DIP controller s General Configuration menu change the mode from ValMode to Operate co N 16
24. ve disconnected the required cables remove the MS front panel carefully please follow the instructions given in the Agilent manual 1 Demount the two boards of the MS MS front panel and then put this two part front panel aside 2 Remove the front panel of the DIP System following the instructions given in the DIP manual chapter 7 2 3 Mount the two boards onto the preset positions of the DIP front panel see marking on the right photo 4 Before mounting the DIP system remove the glass plate in front of the MS MS ion source compartment 5 To mount the DIP system in front of the MS MS first deinstall the valve unit by loosing the two screws see marked circles on the left Now the adapter plate is separated and the four screws are well accessible for the next step Scientific Instruments Manufacturer GmbH D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de 6 Take the adapter plate and mount it in front of the ion source compartment using the four screws see marked circles on the left the two screws behind the motors are not visible on this photo 7 Then retighten the valve unit of the DIP system on the adapter plate with the two screws according to the photo of step no 5 8 Replace the original Knurled knob of the MSD valve with the provided knob for the DIP System see marked rectangle in the photo left 9 Mount the push rod cover by reversing the instructions in chapter 7 2
25. wards 14 Mount the push rod cover and the front panel onto the MSD again and press the arrow right three times so that the valve is closed and initializing of the DIP is started 15 Check the exact position of the push rod by using the service mode Auto 16 Loose the transportation safety screw see figure above Now you can pump down the MS and then start running the DIP E 3 ES IM ie Scientific Instruments Manufacturer GmbH B D 46149 Oberhausen 49 208 94 10 78 0 www sim gmbh de 4 Temperature Calibration of the Controller 4 1 Menu General Configuration This menu is used to select the language German or English Moreover it is possible to change the operational mode which is reserved to the service engineers 1 Note 4 2 Select this menu General Config by pressing the arrow right key until the service menu appears and two subsequent clicks on the arrow right key Press Enter and select with the arrow right key a the language cursor is on the left side with the arrow keys up and down you can change between English and German b the operational mode cursor is on the right side with the arrow keys up and down you can change between Operate normal measuring mode and ValMode validation mode Skip back to the basic display by pressing the arrow right key The validation mode ValMode
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