Qualified Service Operator Manual
Contents
1. In this manual the following conventions are used WARNING Indicates an operation that may cause personal injury if precautions are not followed WARNING All standard safety precautions regarding high voltages vacuum systems and electrostatic discharge must be followed to prevent personal injury or damage to the instrument WARNING Verify with the operator that no biohazardous materials were run through the instrument If these materials have been used ask the operator to use the proper cleaning methods before maintenance WARNING The probes and source housing reach high temperatures Do not remove the probe from the source housing or the housing from the instrument while either is hot Allow at least 10 minutes for it to cool CAUTION Indicates an operation that may cause damage to the instrument if precautions are not followed NOTE Emphasizes significant information in a procedure or description TO TOP PT T of Previous View C Table of Contents Shutting Down Starting Up the Instrument The instrument should be shut down before performing any maintenance in order to minimize the potential for exposure to the instrument s high operating voltages NOTE This procedure should only be used when the instrument is to be serviced For procedures on leaving the instrument overnight see the API 4000 Hardware Manual Procedures outlined in this section include Shutting Down the Instrument Starti2. Qualified Service Operator Manual API 4000 LC MS MS System D1000014710E July 2008 y Applied Analytical AS Biosystems CMDS Technologies This document is provided to customers who have purchased Applied Biosystems MDS Analytical Technologies equipment to use in the operation of such Applied Biosystems MDS Analytical Technologies equipment This document is copyright protected and any reproduction of this document or any part of this document is strictly prohibited except as Applied Biosystems MDS Analytical Technologies Instruments may authorize in writing Equipment that may be described in this document is protected under one or more patents filed in the United States Canada and other countries Additional patents are pending Software that may be described in this document is furnished under a license agreement It is against the law to copy modify or distribute the software on any medium except as specifically allowed in the license agreement Furthermore the license agreement may prohibit the software from being disassembled reverse engineered or decompiled for any purpose Portions of this document may make reference to other manufacturers products which may contain parts whose names are registered as trademarks and or function as trademarks Any such usage is intended only to designate those manufacturers products as supplied by Applied Biosystems MDS Analytical Technologies Instruments for incorp
3. Standing at the left side of the instrument remove the Turbo V source housing by rotating the black locking arms upward to loosen the housing and pulling the housing toward you 8 Remove the dome shaped curtain plate by pulling it straight toward you It is held in place by three retaining balls mounted on the orifice plate 9 Remove the orifice plate by depressing two spring latches behind the collar of the interface housing at the 4 and 10 o clock positions and pulling the orifice plate straight toward you 10 Remove the skimmer cone by pulling it straight from the interface housing 1 Remove the two O rings The front of the QO rods are exposed at this point PT T of Previous View C Table of Contents Cleaning the Vacuum Interface The interface components curtain plate orifice plate and skimmer cone should be cleaned periodically to ensure optimal instrument performance Cleaning will keep the orifice clear reduce electrical noise and prevent samples from adhering to the interface components and altering the electrical performance of the interface The curtain plate orifice plate and the skimmer can be removed and cleaned with methanol and a clean lint free wipe Kim Wipe WARNING If hazardous biohazardous or radioactive materials have been analyzed in the instrument take all necessary precautions as outlined on the MSDS material when cleaning the interface ion source and vacuum chamber
4. Do not allow the tip of the probe to touch anything 2 Tighten the bronze retaining ring 3 Attach the sample inlet line to the ion source probe TO TOP PT F T of revious View C LELI CEROT EA Turbo V Source Housing Use the procedures in this section to remove and replace the Turbo V source housing Removing and Replacing the Turbo V Source Housing You can remove the Turbo V source housing with or without an ion source probe in place You can also remove the Turbo V source housing with the transfer sample tubing attached to an external pump if the tubing is long enough to avoid kinking or stretching TEGI i P llii Turbo V source housing WARNING Do not remove the probe from the source housing or the A N housing from the instrument while either is hot Allow at least 10 minutes for it to cool TO TOP PT T of Previous View C Table of Contents To remove the Turbo V source housing 1 Complete any ongoing scans or select the Abort Sample command from the Acquire menu 2 Shut down the sample flow to the ion source probe 3 Unscrew the sample inlet line that connects the external sample pump to the fitting on the grounding tee and remove the sample inlet line 4 Rotate the two black locking arms upward to loosen the housing Pull the Turbo V source housing straight away from the vacuum chamber To replace the Turbo V source housing 1 Line the Turbo V source housing up
5. Removing and Replacing the Ion Source Probe After disconnecting the sample transfer tubing proceed as follows to remove and replace the TurbolonSpray or APCI probe Bronze retaining ring soe Electrode tip Black collar TurbolonSpray probe Bronze retaining ring gt in Electrode tip Black collar APCI probe WARNING Do not remove the probe from the source housing or the housing from the instrument while either is hot Allow at least 10 minutes for it to cool WARNING Follow this procedure to avoid contact with the high voltages applied to the lonspray inlet and the curtain plate PT E T of revious View C Table of Contents CAUTION To avoid damaging the probe do not allow the nebulizer tip of the TurbolonSpray probe or the corona discharge needle of the APCI probe to touch any part of the source housing To remove an ion source probe 1 Complete any ongoing scans or select the Abort Sample command from the Acquire menu D Shut down the sample flow to the ion source probe oS Unscrew the sample inlet line from the ion source probe and remove the sample inlet line 4 Unscrew the bronze retaining ring Nn Gently pull the ion source probe straight up out of the housing Do not allow the tip of the probe to touch anything as you remove it or store it Place the probe aside in a safe area To replace an ion source probe 1 Slide the ion source probe straight down into the housing
6. To establish communication with the V 902 turbo pump select Setting gt Communication gt Relnit 18 Select V 801 Navigator and click OK 19 If V 801 Navigator does not appear on the list select V 1001 Navigator as its settings are identical 20 To enable the Soft Start function select Setting gt Soft Start gt Enable 21 Select Setting gt Communication gt Stop to end communication NOTE The Soft Start function will remain enabled after communication has stopped and the serial cable connector has been disconnected 22 Turn off the instrument 23 Unplug the mains power cord from the bulkhead at the back right side of the chassis Leave the roughing pump running 24 Push the System Controller module and the DACs and Vacuum Gauge module back into the card cage Finger tighten the locking screws TO TOP PT T of revious View C LELI CEROT EA 25 Plug the mains power cord into the bulkhead at the back right side of the chassis 26 Turn on the instrument The Vacuum On light will begin to flash and the instrument will begin the Soft Start cycle NOTE The instrument can be pumped down overnight in Soft Start mode After approximately 20 minutes the instrument s status light will turn off as the turbos have not reached full speed within the Turbo Timeout window however the instrument will continue to pump down in Soft Start mode The V 902 turbo pump takes the most time to reach full speed 38 KRPM and its status
7. assembly CEM cover Front cover Back cover Power distribution cover Card cage blower filter CEM assembly CEM replacement Roughing pump example Vacuum gauge TO TOP PT T of Previous View C Table of Contents About This Manual This manual identifies the instrument maintenance procedures that should be performed on a periodic basis for the API 4000 instrument These procedures may be performed by a qualified service operators who have been trained in maintenance procedures For more complex system maintenance or assistance with these procedures please contact your Applied Biosystems MDS Analytical Technologies FSE WARNING Ifyou need to move the system contact a FSE to assist you Risk of personal injury or instrument damage NOTE If you are not comfortable with performing any of the procedures outlined here contact your Applied Biosystems MDS Analytical Technologies FSE for assistance The following is a list of user performed maintenance procedures covered in this manual Shutting Down Starting Up the Instrument Maintaining the Turbo V Source Maintaining the Vacuum Interface Cleaning QO Opening Removing the System Covers Removing and Replacing the Card Cage Blower Filter Changing the Channel Electron Multiplier CEM Maintaining the Dry Scroll Roughing Pump Servicing the Vacuum Gauge Soft Starting the Varian Turbo Pumps TO TOP PT F T of Previous View C Table of Contents Conventions
8. can be monitored by selecting Windows gt Monitor It will take approximately one hour to reach full speed Once both pumps have reached full speed Soft Start can be disabled To disable the Soft Start NOTE You cannot disable Soft Start mode while the pumps are running 1 Turn off the instrument 2 Unplug the mains power cord from the bulkhead at the back right side of the chassis Leave the roughing pump running 3 Open the instrument s front cover 4 Loosen the locking screws on the System Controller module and the DACS and Vacuum Gauge module and pull them out It is not necessary to remove the modules just ensure they are fully disconnected This prevents the firmware from starting the pumps as soon as the instrument is powered up 5 Connect the 9 pin serial cable connector provided with the Navigator software to the computer s serial connection port 1 Connect this cable to the 9 pin serial cable attached to the controller for the V 301 pump 6 Plug the mains power cord into the bulkhead at the back right side of the chassis 7 Turn on the instrument 8 Select Start gt Varian Vacuum Technologies gt Navigator 9 To establish communication with the V 301 turbo pump select Setting gt Communication gt Relnit 10 Select V 301 Navigator and click OK TO TOP PT T of Previous View C Table of Contents 11 To disable the Soft Start function select Setting gt Soft Start gt Disable 12 Select Setting gt C
9. its own power toggle switch and must be turned off manually It is not controlled remotely by the system controller 6 Unplug the mains power cord to the instrument from the bulkhead at the back right side of the chassis TO TOP PT T of Previous View C Table of Contents CAUTION If the instrument is to be shut down for any length of time it is recommended that the vacuum chamber be vented to prevent the roughing pump exhaust from being sucked back into the vacuum chamber following instrument shutdown To vent the vacuum chamber follow step 7 CAUTION If the vacuum chamber is not going to be vented while the instrument is shut down it is recommended that the roughing pump s remain turned on to prevent pump exhaust from being sucked back into the vacuum chamber If you do not vent the vacuum chamber skip step 7 7 Let the instrument vent naturally through the orifice for 10 minutes to reach atmospheric pressure The instrument is now ready to be serviced Starting Up the Instrument To power up the instrument after service 1 Place the vent connection on the rear flange and tighten 2 Replace the instrument covers if removed 3 Turn on the roughing pump s if turned off and wait five minutes before proceeding with step 4 NOTE The roughing pump s has its own power toggle switch and must be turned on manually The roughing pump s is not controlled remotely by the system controller Instruments using a singl
10. pump s 3 Disconnect the exhaust vent line from the top of the mist eliminator Loosen the five screws securing the top of the mist eliminator canister A spring holds the filter in place Taking note of the filter s orientation remove the lid Remove the filter Install a new filter in the correct orientation a Position the spring and lid and replace the five screws that secure the lid Servicing the Vacuum Gauge The vacuum gauge must be replaced when the filament voltage required to maintain the 0 1mA electron emission current surpasses 3 V Normally the filament requires 1 5 V to 3 V to maintain the required electron emission current The filament voltage can be read from test point FIL on the DACs and vacuum gauge controller board Normally the green LED on the DACs and vacuum gauge controller lights when the vacuum gauge is enabled However if the filament voltage reaches the 3 V ceiling the LED will not light when the gauge is enabled Procedures outlined in this section include Removing the lon Vacuum Gauge Replacing the Vacuum lon Gauge TO TOP PT T of Previous View C LELI CEROT EAL Removing the Ion Vacuum Gauge WARNING Do not dispose of system components or subassemblies including computer parts in municipal waste Dispose of replaced components and instruments according to established waste electrical equipment procedures To remove the ion vacuum gauge 1 Shut down the instrument and l
11. the Vacuum Interface Vacuum interface maintenance procedures outlined in this section include Opening the Vacuum Interface Cleaning the Vacuum Interface TO TOP T of Previous View C Table of Contents Opening the Vacuum Interface Use the procedures in this section to remove the curtain plate orifice plate and skimmer cone for cleaning or replacement purposes You must also remove the vacuum interface components to access and clean the QO region O Vacuum interface components TO TOP TO TOP PT T of Previous View C LELI CEROT EAL To open the vacuum interface 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To avoid a catastrophic failure of the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Unplug the mains power cord to the instrument from the bulkhead at the back right side of the chassis 4 Let the instrument vent for 10 minutes 5 Loosen the interface connection to the roughing pump to reach atmospheric pressure 6 Disconnect the tubing between the sample pump and the splitter at the left of the source housing 7
12. with the vacuum chamber and push into place 2 Rotate the two black locking arms downward to secure the housing 3 Connect the sample inlet line ferrule to the fitting on the front right of the housing TO TOP PT T of Previous View C LELI CEROT EAL APCI Corona Discharge Needle Use procedures in this section to adjust or replace the corona discharge needle Adjusting the Corona Discharge Needle When using the APCI source ensure that the corona discharge needle is facing back when looking at the source from the front WARNING Follow this procedure to avoid contact with the high voltages applied to the corona discharge needle and the curtain plate To adjust the corona discharge needle 1 Use a slotted screwdriver to adjust the screw on the top of the needle 2 Look through the glass window to ensure that the needle is aligned with the tip facing the orifice If further adjustment is needed 1 Remove the Turbo V source housing For more information see Removing and Replacing the Turbo V Source Housing 2 Turn the housing so that the back is facing towards you 3 Turn the needle so that the tip is pointing towards you You can now adjust the corona discharge needle Replacing the Corona Discharge Needle Care must be taken to ensure your safety from the high voltages applied to the ion source probe WARNING Follow this procedure to avoid contact with the high voltages applied to the corona di
13. PT n T of revious View C Table of Contents 5 Slide the replacement filter into the chassis 6 Close the filter cover 7 Turn the screws 90 clockwise to secure the cover Do not over tighten the screws Changing the Channel Electron Multiplier CEM Procedures outlined in this section include Removing the CEM Replacing the CEM TO TOP TO TOP PT F T of Previous View C Table of Contents Removing the CEM WARNING Follow the shut down procedure to ensure power cord is disconnected WARNING Do not dispose of system components or subassemblies including computer parts in municipal waste Dispose of replaced components and instruments according to established waste electrical equipment procedures To remove the CEM cover 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Unplug the mains power cord to the instrument from the bulkhead at the back right side of the chassis 4 Let the instrument vent for 10 minutes to reach atmospheric pressure 5 Open
14. a suitable container 6 When the oil flow slows replace the drain plug and switch the pump on for not more than 10 seconds TO TOP PT T of Previous View C Table of Contents 7 Remove the drain plug again and allow the remaining oil to drain 8 Replace the drain plug making certain the flat gasket is in good condition and properly in place 9 Remove the oil fill plug 10 Fill the pump with new oil by inserting a funnel in the oil fill inlet hole Pour in the oil to 3 4 inch below the maximum mark 1 liter or 1 05 quarts on the pump sight glass CAUTION Use only the correct amount of oil Overfilling can cause bubbling and backstreaming of oil into the vacuum system Underfilling can cause overheating and decomposition of the oil resulting in pump damage 11 Replace the oil fill plug Cleaning the Filter Trap The roughing pump has a filter trap inside its intake tube If the trap becomes clogged the pump performance will deteriorate Given the controlled atmosphere in the vacuum interface and vacuum chamber it is unlikely that the filter trap will become clogged under normal operating conditions However if the pump performance deteriorates the trap should be examined and cleaned if necessary CAUTION Use cleaning or decontamination methods specified by the manufacturer To clean the filter trap 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at l
15. ce the sample transfer tubing and to remove and replace either type of ion source probe Changing the Sample Transfer Tubing You must remove the sample transfer tubing connection to the TurbolonSpray or APCI probe before removing the probe Use this procedure to remove and replace the tubing between the splitter and the probe Care must be taken to ensure your safety from the high voltages applied to the probe WARNING Follow this procedure to avoid contact with the high voltages applied to the lonspray inlet and the curtain plate To change the sample transfer tubing 1 Complete any ongoing scans or select the Abort Sample command from the Acquire menu 2 Shut off the sample flow to the ion source probe 3 Unscrew the sample transfer tubing ferrule from the ion source probe Unscrew the other end of the sample transfer tubing from the splitter located at the front left of the source housing and remove the tubing 5 Remove both ferrules from the old tubing if necessary so they can be reused with the new tubing Insert a suitable length of replacement tubing through both ferrules 7 Attach each end of the replacement tubing as follows slide each ferrule up the tubing to expose about 3 4 inch of tubing insert the tubing into the splitter or ion source probe handle until it bottoms out and then slide the ferrule down the tubing and tighten it by hand TO TOP PT T of Previous View C Table of Contents
16. components CAUTION Before using any cleaning or decontamination methods except those specified by the manufacturer operators should check with the manufacturer that the proposed method will not damage the equipment CAUTION Do not spray solvent or water through the orifice or into the vacuum chamber CAUTION Take extreme care when cleaning the orifice Clean gently with a clean lint free wipe or swab dampened with methanol NOTE Always wear powder free latex gloves when cleaning the interface components Keep the components and vacuum chamber free of dust and lint Do not use cotton swabs in any of the cleaning procedures since they may leave cotton fibres behind Only use the poly swabs provided with the instrument TO TOP PT T of revious View C LELI CEROT EAL To clean the interface components With the curtain plate orifice plate and skimmer cone removed proceed as follows CAUTION The tip of the skimmer is fragile Handle it very carefully 1 Clean both sides of the curtain plate orifice plate and skimmer cone with 1 1 water methanol or 1 1 water acetonitrile and a clean lint free wipe or poly swab 2 Spray the curtain plate orifice plate and skimmer cone with dry oil free gas to evaporate the residual methanol or allow them sufficient time to dry before reinstalling them 3 Inspect and replace the cleaned curtain plate orifice plate and skimmer cone 4 Replace the cleaned skimmer cone ori
17. e roughing pump require 5 minutes to allow the pump to switch from low speed to high speed 4 Ensure that the Curtain Gas supply is flowing to the instrument The pressure should be regulated to 60 psig 5 Plug the mains power cord to the instrument from the bulkhead at the back right side of the chassis 6 Turn on the main power switch Ensure that the General Purpose Interface Bus GPIB cable is connected to both the API 4000 instrument and the computer 8 Turn on the computer TO TOP PT F T of Previous View C Table of Contents NOTE Should the ion source be removed the system electronics will be disabled interrupting any data acquisition tasks The turbo pumps and the vacuum system will not be affected Maintaining the Turbo V Source The procedures for Turbo V source maintenance are contained in the API 4000 Hardware Manual Turbo V source maintenance procedures outlined in this QSO manual include Ion Source Probe Changing the Sample Transfer Tubing Removing and Replacing the lon Source Probe Turbo V Source Housing Removing and Replacing the Turbo V Source Housing APCI Corona Discharge Needle Adjusting the Corona Discharge Needle Replacing the Corona Discharge Needle TO TOP PT T of Previous View C Table of Contents Ion Source Probe The procedures in this section refer to both the TurbolonSpray probe and the APCI probe Use these procedures to attach or repla
18. east fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of five minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Let the instrument vent for 10 minutes A TO TOP PT F T of Previous View C Table of Contents 4 Loosen the interface connection to the roughing pump to reach atmospheric pressure Remove the vacuum intake line from the pump Lift the filter trap from inside the intake If necessary remove any material in the trap COND Replace the trap and reconnect the vacuum intake line to the pump Replacing the Mist Eliminator Filter If the pump has a mist eliminator installed on the pump exhaust each mist eliminator filter should be replaced periodically WARNING If hazardous biohazardous or radioactive materials are injected into the instrument all appropriate precautions should be _ taken when handling the pump oil and the coalescing filter The oil will be contaminated and should be handled with due caution as if handling the hazardous material itself Deposit biohazardous material in appropriately labelled containers Potential risk of severe personal injury if proper procedures for handling and disposing of biohazardous materials are n
19. eave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of five minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Let the instrument vent for 10 minutes 4 Loosen the interface connection to the roughing pump to reach atmospheric pressure WARNING Ensure the power is disconnected prior to removing the back cover 5 Unlatch the back cover interlock at the front bulkhead assembly The back cover slides off 6 Push back the spring loaded clip from beneath the electrical connection and disconnect the electrical connection at the bottom of the vacuum gauge TO TOP 4 T of Previous View C Table of Contents Vacuum gauge 7 Remove the vacuum gauge assembly complete with its stainless steel elbow by unscrewing the clamp that connects the gauge to the pipe protruding from the vacuum chamber Replacing the Vacuum Ion Gauge To replace the vacuum ion gauge 1 With a clean lint free wipe clean the vacuum fitting on the new gauge 2 Reclamp the vacuum gauge assembly to the pipe protruding from the vacuum chamber 3 Push back the spring loaded clip and reconnect the elec
20. fice plate and curtain plate NOTE Depending on the customer sample type other solvents may be necessary to achieve a proper cleaning Acceptable solvents are 50 50 MeOH H 0 0 1 Acetic Acid Rinse any acid containing cleaning solvents with water Do not use chlorinated solvents TO TOP T of Previous View C Table of Contents Cleaning QO CAUTION Before using any cleaning or decontamination methods except those specified by the manufacturer operators should check with the manufacturer that the proposed method will not damage the equipment The high pressure entrance quadrupole Q0 may become contaminated if the instrument is operated with a Curtain Gas supply setting that is too low Symptoms of a contaminated QO include a dramatic loss in sensitivity typically a factor of 5 and a decrease in ion peak widths A recommended test for Q0 contamination is as follows scan Q1 from 30 to 500 amu with a step size of 0 1 amu and a dwell time of 0 5 msec If there is a cut off in low mass ions e g no signals below mass 100 then QO is most likely contaminated Open vacuum interface TO TOP PT F T of Previous View C Table of Contents To clean QO 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutti
21. he pumps as soon as the instrument is powered up 5 Turn on the instrument s roughing pump 6 Remove the source housing and cover the orifice with a small piece of paper or Teflon tape This will allow the Soft Start procedure to be completed in approximately one hour 7 Connect the 9 pin serial cable connector provided with the Navigator software to the computer s serial connection port 1 Connect this cable to the 9 pin serial cable attached to the controller for the V 301 pump PT T of Previous View C Table of Contents 8 Plug the mains power cord into the bulkhead at the back right side of the chassis 9 Turn on the instrument 10 Select Start gt Varian Vacuum Technologies gt Navigator 11 To establish communication with the V 301 turbo pump select Setting gt Communication gt Relnit 12 Select V 301 Navigator and click OK 13 To enable the Soft Start function select Setting gt Soft Start gt Enable 14 Select Setting gt Communication gt Stop to end communication NOTE The Soft Start function will remain enabled after communication has stopped and the serial cable connector has been disconnected 15 Disconnect the 9 pin serial cable that is attached to the computer from the 9 pin serial cable connector attached to the controller for the V 301 pump 16 Connect the 9 pin serial cable connector that is attached to the computer to the 9 pin serial cable attached to the controller for the V 902 pump 17
22. hree months of operation or when it becomes dirty WARNING Do not dispose of system components or subassemblies including computer parts in municipal waste Dispose of replaced components and instruments according to established waste electrical equipment procedures TO TOP a T of Previous View C Table of Contents The card cage blower filter can be replaced easily without opening or removing any of the instrument covers The filter is accessed through a filter cover located on the chassis below the front cover Card cage blower filter To remove and replace the card cage blower filter 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To avoid a catastrophic failure of the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 With a flat head screwdriver turn the two screws that secure the filter cover plate 90 counter clockwise Do not force the screws they are not threaded into the frame Instead they turn the catch that hooks over the chassis securing the filter cover 4 Lower the filter cover and slide the filter out of the frame TO TOP
23. lier CEM cover encloses the CEM assembly and the vacuum chamber rear flange The cover is hinged to the back of the vacuum chamber and when closed is secured in position by a slotted screw that threads into the vacuum chamber housing The CEM cover must be opened before you can open the front cover WARNING When the CEM cover is open the operator is exposed to the high CEM operating voltage Shut down the instrument and remove the power cord to prevent an electrical shock hazard To open the CEM cover 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Unscrew the slotted screw that secures the cover to the vacuum chamber 4 Swing the CEM cover open TO TOP T of Previous View C Table of Contents Opening the Front Cover Opening the front cover exposes the main components of the API 4000 instrument including many of the system test points The cover is secured at the top by two clasps mounted on top of the QPS coil boxes and it is hinged to the chassis on the two bottom c
24. ng Up the Instrument TO TOP PT T of revious View C LELI CEROT AEA Shutting Down the Instrument To shut down the instrument 1 Complete any ongoing scans or select the Abort Sample command from the Acquire menu 2 Shut off the sample flow to the instrument CAUTION The sample flow must be turned off before shutting down 3 Close the application software 4 Shut off the main power switch to the instrument The switch is located on the bulkhead on the back right corner of the chassis NOTE When the main power switch is turned off the turbo pumps continue to rotate without power for a few minutes and continue to provide vacuum to the vacuum chamber If during this time the roughing pump is powered down the pressure in the vacuum line between the roughing pump s and the turbo pumps increases The increase in back pressure can create an incorrect load on the turbo pump bearings and can cause a catastrophic failure of the turbo pumps CAUTION To prevent damage to the turbo pumps leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 5 Shut off the roughing pump s The pump is located outside the main console The power switch is located beside the power supply input attachment NOTE The pump has
25. ng off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Unplug the mains power cord to the instrument from the bulkhead at the back right side of the chassis 4 Let the instrument vent for 10 minutes 5 Loosen the interface connection to the roughing pump to reach atmospheric pressure Remove the Turbo V source housing Remove the curtain plate orifice plate and skimmer cone with its O rings 8 Gently clean Q0 from the back end to the front to ensure that contaminants are not pushed towards the focusing lens Dampen a poly swab provided with methanol and carefully slide it through the center of the four quadrupole rods Take care not to use excessive force when inserting the swab NOTE Do not use cotton swabs in any of the cleaning procedures since they may leave cotton fibres behind Only use the poly swabs provided with the instrument TO TOP T of Previous View C Table of Contents Cleaning the Q0 rods 9 Allow the methanol on QO to dry NOTE If Q0 is contaminated by proteins or peptides a more aggressive solvent may be needed to clean QO If this is the case use 1 1 water methanol 0 1 acetic acid followed by 100 water and finishing with 100 methanol 10 Replace the skimmer cone and O rings orifice plate and cu
26. ommunication gt Stop to end communication 13 Disconnect the 9 pin serial cable that is attached to the computer from the 9 pin serial cable connector attached to the controller for the V 301 pump 14 Connect the 9 pin serial cable connector that is attached to the computer to the 9 pin serial cable attached to the controller for the V 902 pump 15 To establish communication with the V 902 turbo pump select Setting gt Communication gt Relnit 16 Select V 801 Navigator and click OK NOTE If V 801 Navigator does not appear on the list select V 1001 Navigator as its settings are identical 17 To disable the Soft Start function select Setting gt Soft Start gt Disable 18 Select Setting gt Communication gt Stop to end communication 19 Turn off the instrument 20 Unplug the mains power cord from the bulkhead at the back right side of the chassis Leave the roughing pump running 2 Push the System Controller module and the DACs and Vacuum Gauge module back into the card cage Finger tighten the locking screws 22 Plug the mains power cord into the bulkhead at the back right side of the chassis 23 Turn on the instrument TO TOP lt gt Previous View ee Table of Contents TO TOP
27. oration into its equipment and does not imply any right and or license to use or permit others to use such product names as trademarks All products and company names mentioned herein may be the trademarks of their respective owners Applied Biosystems MDS Analytical Technologies Instruments makes no warranties or representations as to the fitness of this equipment for any particular purpose and assumes no responsibility or contingent liability including indirect or consequential damages for any use to which the purchaser may put the equipment described herein or for any adverse circumstances arising therefrom Applied Biosystems MDS Analytical Technologies Instruments is a joint venture between Applied REGISTERED Biosystems Inc and MDS Inc COMPANY API 4000 CURTAIN GAS and TURBO V are trademarks and TURBOIONSPRAY is a registered trademark of Applied Biosystems MDS Analytical Technologies Instruments a joint venture between Applied Biosystems and MDS Inc Equipment built by MDS Analytical Technologies a business unit of MDS Inc 71 Four Valley Dr Concord Ontario Canada L4K 4V8 Manufactured in Singapore MDS Analytical Technologies and Applied Biosystems are ISO 9001 registered 2008 MDS Inc and Applied Biosystems Inc Joint Owners All Rights Reserved Printed in Canada El gt lt Previous View Qualified Service Operator Manual API 4000 LC MS MS System Table of Contents Table of Contents List of Fig
28. orners Front cover To open the front cover 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Unscrew the slotted screw that secures the CEM cover to the vacuum chamber TO TOP f T of Previous View C Table of Contents 3 Swing the CEM cover open 4 Using a Phillips screwdriver unlatch the front cover interlock at the front bulkhead assembly 5 Grasp the top corners of the front cover and gently pull on the cover to release the two clips that secure it 6 Lower the cover Removing the Back Cover The back cover encloses most of the system cabling the mother board and the vacuum gauge It is not hinged to the chassis and must be removed to access the back of the API 4000 instrument Back cover WARNING Shut down the instrument and remove the power cord prior to removing the back cover to prevent electrical shock hazard TO TOP PT T of Previous View C Table of Contents To remove the back cover 1 Shut down the instrument and leave the roughing pump s running with the instrument t
29. ot followed To replace a Leybold mist eliminator filter 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Unscrew the thumb screw on top of the mist eliminator and lift away the top section of the eliminator to expose the filter 4 Lift the filter out of the top of the mist eliminator housing 5 Install a new filter 6 Replace the top section of the mist eliminator housing and replace the thumb screw TO TOP PT T of Previous View C Table of Contents To replace a Varian 949 9392 mist eliminator filter 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes 2 Shut off the roughing pump s CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing
30. riate precautions should be taken when handling the pump oil and the coalescing filter The oil will be contaminated and should be handled with due caution as if handling the hazardous material itself Deposit biohazardous material in appropriately labelled containers Potential risk of severe personal injury if proper procedures for handling and disposing of biohazardous materials are not followed CAUTION The pump oil should be changed when the pump is warm Use the replacement oil specified on the pump identification tag To change the roughing pump oil you need the following equipment and parts e 8 mm Allen key e 1 5 to 2 liter oil collection container e Replacement oil To replace the pump oil 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Let the instrument vent for 10 minutes 4 Loosen the interface connection to the roughing pump to reach atmospheric pressure 5 While the roughing pump is still warm remove the drain plug with an 8 mm Allen key and drain the pump oil into
31. rtain plate 11 Replace the Turbo V source housing TO TOP PT T of Previous View C Table of Contents Opening Removing the System Covers There are three covers enclosing the operating modules of the API 4000 instrument main console The covers may be opened to provide access to the instrument s component modules system electronic box and operational parameter check points The covers are designed with a mechanical interlock to prevent access to the instrument unless a tool is used to open the doors WARNING Shut down the instrument and remove the power cord prior to removing any covers requiring the use of tools WARNING The interlocks are mechanical latches only They do not Shut down power within the instrument When the front and back covers are open all voltages are still present and the instrument is operational Procedures outlined in this section include Unlatching the Front Bulkhead Assembly Interlocks Opening the Channel Electron Multiplier CEM Cover Opening the Front Cover Removing the Back Cover The Power Distribution Cover TO TOP f T of Previous View C Table of Contents Unlatching the Front Bulkhead Assembly Interlocks As shown below the front bulkhead assembly is a fixed cover located beneath the Turbo V source housing Front bulkhead assembly The front bulkhead assembly located at the left side of the instrument under the Turbo V source housing encloses the so
32. s when the pump is operating at close to its ultimate pressure If the pump oil falls outside these boundaries oil should be added or drained until it reaches the appropriate level The pump manufacturer recommends that the pump oil be changed under the following conditions e When the oil is contaminated e Before and after the pump is stored for a lengthy period The condition of the pump oil can be checked while the pump is operating When in good condition the oil will appear light in color and be relatively clear If the pump oil becomes dark appears dirty or excessively turbid the oil should be changed TO TOP PT T of Previous View C LELI CEIRO EA The roughing pump a typical example is shown below has a sight gauge for determining both the oil level and condition A aust Port Intake Port Oil Fill Plug Exhaust Port Y Intake Port Oil Sight Gauge Oil Drain Plug Gas Ballast Control Power On Off Switch Handle Roughing pump example Procedures outlined in this section include Replacing the Roughing Pump Oil Cleaning the Filter Trap Replacing the Mist Elimintator Filter TO TOP PT T of Previous View C Table of Contents Replacing the Roughing Pump Oil WARNING If hazardous biohazardous or radioactive materials are injected into the instrument all approp
33. scharge needle and the curtain plate WARNING Do not dispose of system components or subassemblies including computer parts in municipal waste Dispose of replaced components and instruments according to established waste electrical equipment procedures PT T of Previous View C LELI CEROT EAL To replace the corona discharge needle 1 Complete any ongoing scans or select the Abort Sample command from the Acquire menu 2 Allow sufficient time for the probe to cool down then remove the sample tubing 3 Remove the ion source probe For more information see Removing and Replacing the lon Source Probe 4 Remove the Turbo V source housing For more information see Removing and Replacing the Turbo V Source Housing 5 Unscrew the plastic screw at the top of the needle while holding the needle NOTE Take care not to lose the spring located between the plastic screw and the needle Gently remove the needle through the exhaust socket Insert the replacement corona discharge needle through the exhaust socket 8 Slide the needle into the holding tube and turn the plastic nut at the top of the needle until a firm connection is made You can now reassemble the source housing insert the ion source probe and begin optimization procedures For information about optimization procedures refer to the API 4000 LC MS MS System Service Manual TO TOP PT T of Previous View C Table of Contents Maintaining
34. ssembly to the feedthroughs a Firmly push the CEM mounting board against the feedthroughs to ensure a sound connection b Gently push the CEM PCB back towards the guide arm 5 Replace the hex head screws that secure the CEM mounting board to the vacuum flange 6 Install the CEM module by sliding the CEM housing straight into the vacuum chamber 7 Replace the two hex head screws that connect the CEM housing to the vacuum chamber 8 Connect the CEM signal and control panel cable to the signal handling board SIGNAL HANDLING VACUUM FLANGE SIGNAL HANDLING GUARD FEED THROUGHS CEM replacement TO TOP PT T of Previous View C Table of Contents Maintaining the Dry Scroll Roughing Pump Dry scroll roughing pumps from Varian require that the scroll module and filter element be replaced at two year intervals Dry scroll roughing pumps from Edwards require that the tip seals be replaced after every year or 9 000 hours of continuous use Please contact your local AB Service Representative if you have any questions Maintaining the Rotary Vane Roughing Pumps The routine maintenance of the roughing pump involves maintaining the level and quality of the pump oil If the pump oil level drops or the oil becomes contaminated the pump performance will deteriorate and the pump will suffer increased wear and may ultimately seize The pump oil level should be maintained between the marks on the oil sight glas
35. the CEM cover as shown below CAUTION The CEM is fragile Use care when installing the new CEM assembly Do not bump the horn or allow dust or dirt on the CEM assembly or the vacuum feedthroughs Contamination can cause electrical noise f T of Previous View C Table of Contents CEM assembly E 6 Disconnect the CEM signal and control panel cable from the signal handling board CAUTION Do not disconnect the CEM cable while voltage is applied to the CEM since it may cause damage to the signal handling board and the system controller 7 Using a 4mm Allen key remove the two hex head screws that connect the CEM housing to the vacuum chamber 8 Slide the CEM housing straight out of the vacuum chamber until the guide arm clears the rear flange 9 Place the CEM assembly carefully on a flat work space with the signal handling guard flat on the table TO TOP f T of Previous View C Table of Contents Replacing the CEM NOTE Wear powder free latex gloves when replacing the CEM To replace the CEM 1 Remove the CEM module and place it on a clean work surface 2 With a 3 mm Allen key remove the hex head screw and associated lock washer connecting the CEM mounting board to the stand off on the vacuum flange CEM assembly 3 Pull the CEM mounting board to release it from the four feedthroughs TO TOP PT T of revious View C Table of Contents 4 Without touching the CEM horn attach a new CEM a
36. trical connection to the gauge 4 Reposition the spring loaded clip beneath the electrical connection TO TOP TO TOP PT T of Previous View C Table of Contents Soft Starting the Varian Turbo Pumps CAUTION If the instrument s Varian turbo pumps have just been replaced or have not been used in at least thirty days a Soft Start procedure will be required to prevent damage to the turbo pumps NOTE Each instrument shipped will have the Soft Start function enabled This ensures that the first pumpdown at the customer site is done under Soft Start conditions To verify that Soft Start has been enabled or to re enable Soft Start following an instrument shutdown of more that 30 days follow the procedure below Once an instrument s turbo pumps have experienced a successful Soft Start the Soft Start procedure should be disabled To install the Navigator Software 1 Insert disk 1 of 2 into the computer 2 Click on Setup 3 Follow the setup instructions inserting the second disk when prompted To Soft Start the turbo pumps 1 Turn off the instrument 2 Unplug the mains power cord from the bulkhead at the back right side of the chassis 3 Open the instrument s front cover 4 Loosen the locking screws on the System Controller module and the DACs and Vacuum Gauge module and pull them out It is not necessary to remove the modules just ensure they are fully disconnected This prevents the firmware from starting t
37. urce exhaust pump The front bulkhead assembly is a fixed cover that does not open It contains two tabs that fit into the front and back cover interlocks The interlocks must be unlatched using a Phillips screwdriver one interlock releases the front cover the other releases the back cover WARNING The interlocks are mechanical latches only They do not shut down power within the instrument When the front and back covers are open all voltages are still present and the instrument is operational TO TOP f T of Previous View C Table of Contents To unlatch the front bulkhead assembly interlocks 1 Shut down the instrument and leave the roughing pump s running with the instrument turned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Using a Phillips screwdriver turn the selected interlock screw head 90 counterclockwise NOTE The screwheads are located at the top corner of the end panels on the front and back covers Opening the Channel Electron Multiplier CEM Cover CEM cover TO TOP PT T of Previous View C Table of Contents The channel electron multip
38. ures About This Manual Conventions Shutting Down Starting Up the Instrument Shutting Down the Instrument Starting Up the Instrument Maintaining the Turbo V Source lon Source Probe Changing the Sample Transfer Tubing Removing and Replacing the lon Source Probe Turbo V Source Housing Removing and Replacing the Turbo V Source Housing APCI Corona Discharge Needle Adjusting the Corona Discharge Needle Replacing the Corona Discharge Needle Maintaining the Vacuum Interface Opening the Vacuum Interface Cleaning the Vacuum Interface Cleaning Q0 Opening Removing the System Covers Unlatching the Front Bulkhead Assembly Interlocks Opening the Channel Electron Multiplier CEM Cover Opening the Front Cover Removing the Back Cover The Power Distribution Cover Removing and Replacing the Card Cage Blower Filter EI Cc Previous View Changing the Channel Electron Multiplier CEM Removing the CEM Replacing the CEM Maintaining the Dry Scroll Roughing Pump Maintaining the Rotary Vane Roughing Pumps Replacing the Roughing Pump Oil Cleaning the Filter Trap Replacing the Mist Eliminator Filter Servicing the Vacuum Gauge Removing the lon Vacuum Gauge Replacing the Vacuum lon Gauge Soft Starting the Varian Turbo Pumps P 3 o lt gt lt l Previous View TP Tabie of Contents List of Figures TurbolonSpray probe APCI probe Turbo V source housing Vacuum interface components Open vacuum interface Cleaning the QO rods Front bulkhead
39. urned off for at least fifteen minutes CAUTION To prevent damage to the turbo pumps you must leave the roughing pump s running for a minimum of fifteen minutes after shutting off the instrument s main power switch to allow the turbo pumps to spin down gradually This prevents the build up of pressure in the vacuum lines from the turbo pumps to the roughing pump s 2 Shut off the roughing pump s 3 Turn off the computer 4 Disconnect the IEEE 488 cable and the AUX I O cable if attached from the back of the instrument 5 Unlatch the back cover interlock at the front bulkhead assembly 6 Grasp the top corners of the back cover and gently pull it away from the vacuum chamber to release the top clips 7 Lift the cover slightly to unseat it from the chassis while pulling it directly away from the vacuum chamber and guiding it past the turbomolecular pumps housings and hoses TO TOP PT T of Previous View C Table of Contents The Power Distribution Cover WARNING There are no user serviceable items located behind the power distribution cover Do not attempt to remove it Power distribution cover Removing and Replacing the Card Cage Blower Filter The two fans that are housed in the card cage blow air over the circuit boards in the system electronics box An air filter that purifies the card cage blower s intake air is inserted into the chassis below the fans The filter should be replaced after every t
Download Pdf Manuals
Related Search