Instruction Manual - Cole
Contents
1. RO 37 8 3 Unable ta set display tO Bet o acociicnnonderidnidc onda ar aiana Sr NANNA NEN 38 8 4 Unable to set display to standard reading ss s sssssssivisssnsssnssnss rr 38 8 5 Unstable PESOS iba ENN SER 38 8 6 DiGi URSA TESM ES a nie a a Aaa iaa E 39 Spares and ACUESTA E EE o ds R R RER E 40 cml Ordering E RN 40 9 2 Spares ANG ACES OTI conocio OR EE E SES NR SR ha SR ARN SRA 40 9 3 pandard ac oussnncescenadeeivaeesacthaieav sacs Fr bred OF Un SRA FNS dass ai ON be KeA nb nr NA 41 PGCE S cecal ss costed R E T E E ddcbaneuaadvane SERNER FARS SRS AR RN AA 42 MILE ate AN 1 1 1 2 Model 2655 00 Flame Photometer Introduction Introduction The Cole Parmer Model 2655 00 is a single channel low temperature flame photometer for the determination of Na K and Li in clinical samples Information on Ca and Ba determinations is also available from your Cole Parmer distributor The Model 2655 00 utilises a digital display and is designed for reliable trouble free operation and ease of maintenance An automatic sample dilutor Model 805 is available The Model 2655 00 has a fail safe device which automatically stops the gas flow if the flame does not ignite or if during operation the flame is extinguished Plus an air pressure switch so that if the air pressure falls below a specified value the flame will not ignite or will be extinguished Summary of the Test NOTE References are listed in Appendix A Prior to the advent of2. spare for mixing chamber 1 400 27 001 Nebuliser 1 400 22 003 Nebuliser inlet tube polythene 150mm 1 410 91 001 Operators Manual English 1 001 08 718 Unex tubing clamp 4 001 56 120 Flame Photometer Standard 1000ppm Na 1 bottle 500 ml 001 56 121 Flame Photometer Standard 1000ppm K 1 bottle 500 ml 001 56 122 Flame Photometer Standard 1000ppm Li 1 bottle 500 ml Assembly EQUIPMENT REQUIRED Pozidriv cross head screwdriver IPT Flat blade screwdriver To assemble the Model 2655 00 proceed as follows 1 Lift off the chimney cap from the chimney assembly and lift out the glass chimney Remove any packing material from inside making sure that there are no small pieces left within the chimney Replace the glass chimney with the clear strip at the bottom and refit the chimney cap on top of the chimney Remove the nebuliser from its box and screw it to the connector on the end of the air tubing item 3 Figure 2 1 Fit the nebuliser to the mixing chamber and position the retainer to lock it into position item 2 Figure 2 1 Fit the nebuliser an lat tanha malina mira that it ta nnintinag Anwmuararda Model 2655 00 Flame Photometer Installation continued 2 4 Assembly continued Figure 2 1 Mixing Chamber 1 Nebuliser 2 Nebuliser retainer 3 Air tubing 4 Constant head drain 5 Fuel restrictor 6 Fuel tubing 4 If the instrument is to be used on Natural gas proceed with paragraph 11 If
3. Air regulator Control to adjust the pressure of the air supply The air pressure gauge provides a visual indication of the air pressure Model 2655 00 Flame Photometer Operating Instructions continued 5 3 Initial Adjustment NOTE The following instructions assume that the air compressor is switched on and l 10 11 12 13 supplying the instrument with air Check that the air pressure gauge on the rear panel indicates a reading between 11 and 13psig If it does not lower the air regulator locking ring and adjust the regulator for a reading of 1 psig on the air pressure gauge Raise the locking ring to lock the air regulator adjuster WARNING The air compressor should always be switched on before the flame is ignited The gas will not flow without sufficient air pressure Check the Gas Trap U tube is filled with water refer to Section 2 4 paragraph 18 Turn the fuel control fully clockwise to the closed position but do not force it This control is a precision assembly that will be damaged by rough handling WARNING Always check that the gas installation is leak proof before initiating an ignition cycle Open the fuel control the required number of turns counterclockwise as follows Fuel No of Turns Propane 9 Butane 11 Natural Gas 16 Turn on the fuel supply at source Depress the power switch on the front panel The power on LED will illuminate and an ignition cycle will commence If the flame
4. Propane or Butane is to be used continue with paragraph 5 5 Connect the length of rubberised fuel tubing 001 72 114 supplied between the inlet connector on the Propane regulator item 5 Figure 2 2 and the cylinder regulator outlet connector Secure with Unex tubing clamps supplied Ensure that the connector at the regulator inlet is tightened to 12nm torque Continue with paragraph 15 NB The connection at the cylinder end of the tube must comply with National regulations 6 Remove the fuel restrictor item 5 Figure 2 1 and connect the fuel tubing item 6 Figure 2 1 directly to the mixing chamber connection de Unscrew and remove the gas inlet regulator inlet and regulator outlet connectors items 4 5 and 6 Figure 2 2 and remove the copper tubing item 2 Figure 2 2 8 Connect the regulator inlet with connector item 5 Figure 2 2 to the gas inlet item 4 Figure 2 2 Model 2655 00 Flame Photometer Installation continued 2 4 Assembly continued Figure 2 2 Rear Panel 1 Natural gas regulator assembly see Figure 2 3 2 Copper tubing 3 Air regulator adjuster 4 Gas inlet connector 5 Regulator inlet connector 6 Regulator outlet connector 9 Locate the Natural gas regulator and mounting bracket ordered as optional accessories and packed separately from the instrument Fit the mounting bracket to the rear panel with the two screws supplied and slot in the Natural gas regulator Figure 2 3 Make s
5. flame photometry Sodium and Potassium were typically determined gravimetrically after the precipitation of relatively insoluble salts such as Sodium Uranyl Zinc Acetate Ref 1 and Potassium Chloroplatinate Ref 2 As with all chemical methods for these two elements there were cross interferences and also interference from other ions such as NH4 Many analytical steps such as protein precipitation or ashing of the sample were involved with all the attendant losses and inaccuracies and the complete procedures required many hours In many applications rapid availability of results is of prime importance By flame photometry both sodium and potassium results on a single sample can be available in less than 5 minutes of the sample reaching the laboratory The same is true of lithium results a determination that was never carried out before the advent of flame photometry A simple dilution step is all that is required therefore sample handling losses and inaccuracies are at a minimum Ref 3 10 Model 2655 00 Flame Photometer Introduction continued 1 3 Reagents Cole Parmer supply a wide range of reagents including standards diluent and maintenance solutions for use with the Model 2655 00 Flame Photometer Please refer to Section 9 3 for a complete list of the reagents available Dilutions Samples and standards must be diluted with the same batch of diluent made up of 1 part Diluent Concentrate to 999 parts deionised or good qu
6. on LED has not illuminated before the end of the ignition cycle switch off and wait ten seconds Then without adjusting the fuel control in case the instrument fuel system is not yet completely filled switch on again to initiate another ignition cycle Repeat this cycle twice to allow sufficient time for the fuel to reach the burner then continue to paragraph 9 If the flame on LED has not illuminated before the end of the ignition cycle switch off the power switch If using Natural Gas continue with paragraph 10 If using Propane Butane continue with paragraph 11 Remove the natural gas regulator screw cap item 1 Figure 2 3 Rotate the screw head fully clockwise and then counterclockwise exactly one and a half turns Refit the screw cap WARNING Do not exceed this setting as this will lead to a very rich fuel mixture which may cause a flame to appear above the chimney Open the fuel control one turn counterclockwise Switch on again If the flame on LED still does not illuminate repeat paragraph 11 Do not open the fuel control more than a total of 4 turns from the settings shown in paragraph 3 If the flame on LED still does not illuminate switch off and refer to Section 8 2 Troubleshooting wna a1 A Ton 1 a 1 cut a at mA A Model 2655 00 Flame Photometer Operating Instructions continued 5 4 Operating Instructions NOTE For greatest accuracy and stability Cole Parmer recommend that a batch of diluent is made
7. safe Check hosing joints with a soap solution before allowing any naked flame in the vicinity Never open a cylinder valve to atmosphere even on a supposedly empty cylinder The chimney cap and glass chimney and the area above the chimney can become very hot and are capable of causing severe burns Never view the flame from the top of the chimney always use the inspection hole Make sure that the air compressor is connected to the power supply and switched on before starting a flame ignition sequence Failure to observe this precaution may result in a build up of fuel gas which will cause a flame to appear above the chimney 7 1 7 2 7 3 7 4 Model 2655 00 Flame Photometer Maintenance General Under the Daily Weekly Monthly and Six Monthly Maintenance headings are summaries of the work and equipment required The tasks are detailed from Section 7 6 onwards For maintenance of the air compressor dilutor and chart recorder if used refer to the instructions supplied with the equipment Daily Maintenance EQUIPMENT REQUIRED None 1 Empty waste container if used 2 Check air line for condensation and drain if necessary 3 Check U tube is filled with deionised water Weekly Maintenance EQUIPMENT REQUIRED 10 ml beaker Stop watch Nebuliser cleaning wire Deproteinising Solution Stainless steel nebuliser tube and sleeve or polythene inlet tube as required 1 Carry out Daily Maintenance procedure
8. the Model 2655 00 Use a dedicated Compressor such as the Model 851 or Model 855 Check the concentration of the solutions being aspirated The solutions should all contain 1 in 1000 dilution of Diluent Concentrate refer to Section 5 4 Check the fuel supply is adequate Make sure the fuel control is set correctly refer to Section 5 3 The use of Natural Gas from a communal source will fluctuate as other users turn on or turn off their gas taps Wherever possible use bottled gas The nebuliser may be blocked Check the nebuliser refer Section 7 6 The U tube or drain tubing may be blocked or partially obstructed If the air supply tubing show signs of condensation drain the air tubing If this problem recurs frequently use a Model 856 air compressor with water separator Make sure the air compressor filter is changed regularly refer air compressor manual If the atmosphere surrounding the flame photometer or air compressor inlet is contaminated e g smoke unstable readings may result A similar effect may be observed if the instrument is sited in a draught If fault persists contact your Cole Parmer Distributor Model 2655 00 Flame Photometer Troubleshooting continued 8 6 Non linear results Check the concentration of the solutions being aspirated The solutions should all contain 1 in 1000 dilution of Diluent Concentrate refer Section 5 4 Make up fresh solutions and recheck calibration curve Check that
9. up from 1 part Diluent Concentrate and 999 parts deionised or good quality distilled water Using diluent for setting blank and for dilution of samples and standards means that all solutions presented to the instrument contain the stability promoting ingredients contained in the diluent Always use the same batch of diluent for the blank and the dilution of samples and standards Store the diluent in a sealed container for not more than five days WARNING Always check that the gas installation is leakproof before initiating an 10 11 12 13 ignition cycle Turn on the fuel supply at source Switch on the Air Compressor Depress the power switch to switch on the instrument The power on LED will be illuminated and an ignition cycle will commence If the flame on LED is not illuminated at the end of the ignition cycle refer to Section 5 3 and check the setting of the fuel control Set the filter selector to the required position Insert the nebuliser inlet tube in a beaker containing 100ml of diluent and allow 30 minutes for the operating temperature to stabilize This will ensure a stable burner temperature when solutions are aspirated after the warm up period During the warm up period prepare a set of calibration solutions to cover the required measurement range To obtain maximum linearity Cole Parmer recommend that the highest standard concentration does not exceed 10ppm for Na K and Li While aspirating diluent adju
10. 0 consecutive readings of the same bulk sample aspirating sample for 20 seconds then 10 seconds of air The concentration of the bulk sample to be 10ppm for Na K and Li which will give a standardized reading of 100 on the display i e 10 0 with decimal point on Warm Up To achieve the stated specification the flame must be alight for a minimum of 30 minutes with diluent being aspirated Sample Requirement Type Dependent on application However samples should be water based and not highly viscous nor non homogeneous Organic solvents affect the air fuel ratio could attack the mixing chamber materials and can affect the safety operation of the Gas Trap constant head drain and should be used with great caution Method of presentation The sample is presented to the nebuliser from a sample cup test tube or other suitable container Volume The maximum diluted sample volume required for 20 seconds aspiration is 2ml Chart Recorder Output Nominal 100mV signal output per 1000 display units Model 2655 00 Flame Photometer Performance Characteristics and Specification continued 4 8 4 9 4 10 4 11 4 12 Environmental Conditions Temperature Operating 10 C to 35 C Transportation 40 C to 45 C Humidity Operating 85 maximum at 35 C Transportation 95 maximum at 45 C non condensing The instrument specification will be unaffected by an ambient temperature change of 4 C or less per hour withi
11. 30psig flow rate at least 0 4 litres per minute The use of industrial quality gas is not recommended as impurities can enter the delicate gas regulators and can leave deposits of oil and dirt which will render the instrument inoperable Natural gas at 3 to 10 inches water gauge regulated to 2 inches water gauge can also be used Natural gas and camping gas regulators are available as optional accessories refer to Section 9 2 NB The positioning of Gas cylinders should conform to National and local regulations Air A supply of clean dry oil free air at 1kg cm_ 14psig flow rate 6 litres per minute A suitable air compressor is listed in Section 9 2 Waste Container A sink or waste container sited to the right of the instrument will ensure the minimum length of waste tubing Do not use a waste container with high sides as this will cause the drain tube to be lifted above the level of the constant head drain Model 2655 00 Flame Photometer Installation continued 2 2 Site Conditions WARNING Under no circumstances install the instrument beneath overhanging cupboards There must be at least I metre of clear space above the chimney For optimum performance this instrument should be installed in accordance with the following conditions 1 The environment must be clean and free from dust 2 The instrument must be placed on a strong level worktop free from vibration The Model 2655 00 requires approximately 500mm x 500
12. 9 199 9 with decimal point on NOTE If negative values are displayed the instrument is operating outside of the recommended measurement range Measurement Ranges Na 0 10ppm linear Above this range Na is non linear due to self absorption A lineariser can be fitted which will linearise concentrations up to 60ppm Na Part number 47829800 K Li amp Ca 0 to 100ppm Ba 0 3000ppm These are sample concentrations directly entering the flame photometer after dilution Specificity For Na K and Li measurements interference will be less than 0 5 from a concentration of Na K Li Ca or Ba equal to the concentration of the element under test Accuracy Linearity Better than 2 measured at mid range when standardized at the following concentrations 3ppm Na 3ppm K amp Sppm Li Drift At zero Better than 2 per hour when calibrated at 10ppm for Na K and Li At 10ppm Better than 2 per 5 minutes for Na K and Li based on 10 consecutive samples aspirating sample for 20 seconds then 10 seconds of air NOTE A minimum 30 minutes warm up must be allowed to meet the drift specification refer to Section 4 5 Model 2655 00 Flame Photometer Performance Characteristics and Specification continued 4 4 Accuracy continued Reproducibility 4 5 4 6 4 7 NOTE The following specification is correct for use on Propane or Butane If Natural Gas is used the reproducibility may be slightly worse Less than 2 CV for 2
13. Decimal pushbutton 9 Power switch 10 Power on LED 11 Flame on LED Model 2655 00 Flame Photometer Operating Instructions continued 5 2 Rear Panel Controls and Connectors Figure 5 2 Rear Panel 1 Power connector and voltage selector 2 Gas inlet connector 3 Data output sockets 4 Air inlet connector 5 Air pressure gauge and air regulator adjuster 6 Propane Butane regulator inlet connector Model 2655 00 Flame Photometer Operating Instructions continued 5 2 Rear Panel Controls and Connectors continued IEC symbol advising user to refer to accompanying documentation Serial Plate symbol denotes equipment suitable for alternating current only Power Three pin connector for the a c supply lead incorporating a voltage selector and fuse compartment Data output Two colour coded chart recorder sockets The nominal 100mV 1000 display digits output signal is on the red socket with respect to the black socket Gas 1 4 inch fuel inlet connector to the instrument permanently connected to the cylinder regulator outlet The instrument will operate satisfactorily on Propane and Butane or Natural Gas using the natural gas regulator available as an optional accessory refer to Section 9 2 See Section 4 10 for pressure and flow rate specifications Air 5 16 inch connector for the air inlet tubing to the instrument from the air compressor outlet See Section 4 11 for pressure and flow rate specifications
14. Model 2655 00 Flame Photometer Operator Manual Copyright 2001 Cole Parmer Instrument Company All rights reserved Model 2655 00 Flame Photometer Intended Use The Model 2655 00 is a single channel low temperature flame photometer for the determination of Na K and Li This operator s manual contains complete instructions for setting up and using the Model 2655 00 Service information for use by appropriately qualified personnel is also available The Model 2655 00 is intended for use by persons knowledgeable in safe laboratory practices If the instrument is not used in accordance with these instructions for use the protection provided by the equipment may be impaired WARNING The Model 2655 00 is designed to be grounded through the power supply lead line cord for safe operation For the safety of operating personnel and optimum performance make sure that the instrument is only connected to a 3 prong socket outlet that has an effective earth connection If you are in any doubt about the safety of your electrical supply system consult a competent qualified electrician There are no user replaceable parts within the instrument Do not remove the rear cover from the instrument Cole Parmer Instrument Company and its authorized Distributors and Agents consider themselves responsible for the effects of safety reliability and performance of the Model 2655 00 only if e Assembly operations extensions re adjustments modification
15. Ze Deproteinising the system Section 7 10 3 Check the operation of the nebuliser Section 7 6 Monthly Maintenance EQUIPMENT REQUIRED As for weekly maintenance 1 Carry out Daily and Weekly Maintenance procedures 2 Check the constant head drain mixing chamber U tube and drain tube and clean if necessary Section 7 7 Check that the U tube is refilled with deionised water on reassembly Model 2655 00 Flame Photometer Maintenance continued 7 5 Six Monthly Maintenance EQUIPMENT REQUIRED As for weekly maintenance plus the following U tube and drain tube Methanol tissues Soft lint free cloth Cotton buds 7 6 Carry out the Daily and Weekly Maintenance procedures 2 Clean the mixing chamber burner tube burner and constant head drain Section 7 7 3 Replace stainless steel nebuliser tube and sleeve or polythene nebuliser tube if fitted U tube and drain tube 4 Clean the optical filters and the glass chimney Section 7 8 5 Check the air and fuel tubing and connectors for leaks using soap solution Check for signs of stress cracking especially at the connectors Nebuliser EQUIPMENT REQUIRED Stop watch 10 ml beaker Cleaning wire Cleaning Solution Stainless steel nebuliser tube and sleeve or polythene inlet tube as required Operational Check Turn off the fuel supply at source Switch on the M2655 00 and the air compressor Check that the flame on LED Fill the beake
16. ality distilled water The same batch of diluent should be used to zero the instrument and to prepare dilutions of standards and samples This will prevent variations in water purity affecting the measurements Great care should be taken so that contamination does not occur when preparing the samples and standards Remember that the accuracy of the instrument is dependent on the accuracy and purity of the standards used for calibration Storage All solutions should be stored away from direct sunlight in a cool place below 25 C 77 F in an airtight container to prevent evaporation and discolouration Glass containers should not be used as they can affect Na concentration levels Prolonged exposure to the atmosphere must be avoided to prevent evaporation of standard solutions which could affect concentration Purification No purification is required for Cole Parmer standard solutions 2 1 Model 2655 00 Flame Photometer Installation Services Required Electrical Supply An a c supply at 100V 120V 220V or 240V 10 at 50 or 60Hz is required for the Model 2655 00 The instrument operating voltage is shown on the voltage selector on the rear panel CAUTION fthe voltage setting is not correct for the local supply do not attempt to use the instrument until it is correctly adjusted as detailed in Section 2 4 paragraph 23 Fuel A supply of Propane Butane or Propane Butane mixture regulated at the cylinder to 2 1kg cm_
17. connected to a working a c supply and that the power switch is depressed Check the plug fuse and replace if necessary Check the instrument fuses and replace if required refer to Section 7 9 If fault persists contact your Cole Parmer Distributor Flame on LED not illuminated General Ensure that the U tube is filled with deionised water refer to Figure 2 4 Burner must be fitted with pointed end downwards refer to Section 7 7 Air Supply Air compressor must be connected to an a c supply and working correctly Check the air tubing connections from the air compressor to the instrument refer to the air compressor manual Fuel Supply There must be a compressed air flow otherwise the air pressure switch will not allow the gas to flow into the instrument Check that there is an adequate supply of fuel Fuel supply must be turned on at source If using a long length of fuel tubing try repeating the ignition sequence a few times to allow the fuel to fill the tubing If using natural gas check the setting of the natural gas regulator refer to Section 5 3 If the fuel is propane or butane check that the regulator at the cylinder is adjusted for 2 1kg cm_ 30psig Check the fuel control is set correctly refer to Section 5 3 If the fuel restrictor propane butane only is blocked clean with the nebuliser cleaning wire refer to Figure 2 1 If fault persists contact your Cole Parmer Distributor Model 2655 00 Flam
18. d from the a c supply socket 3 Disconnect the a c supply lead from the power connector on the rear panel 4 Open the voltage selector compartment and withdraw the voltage selector item 1 Figure 7 3 To replace fuses continue with paragraph 5 to change the operating voltage continue with paragraph 7 5 Slide out the two fuse holders item 2 Figure 7 3 and remove the fuses Replace any burned out fuses and slide in the fuse holders Replace the voltage selector so that the required voltage setting is visible through the window of the compartment cover Close the compartment cover and refit the a c supply lead Figure 7 3 Voltage Selector and Fuse 1 Voltage selector 2 Fuse holders Model 2655 00 Flame Photometer Maintenance continued 7 10 Deproteinising or Disinfecting Procedure NOTE To deproteinise the system use Deproteinising Solution to disinfect the system use Tubing Disinfectant EQUIPMENT REQUIRED Deproteinising Solution or Tubing Disinfectant 1 Light the flame as detailed in Section 5 4 2 Present a beaker of Deproteinising Solution or Tubing Disinfectant to the nebuliser for 10 minutes 3 After 10 minutes have elapsed replace the beaker of solution with a beaker of deionised water Flush the system for two minutes 4 Shutdown the instrument as detailed in Section 5 5 8 1 8 2 Model 2655 00 Flame Photometer Troubleshooting Power on LED not illuminated Check that the instrument is
19. drain Fit the drain tube to the constant head drain Fit the nebuliser into the end cap and position the retainer to lock it Use a wash bottle to fill the U tube with deionised water Sufficient water should be used to completely fill the U tube and purge it of air Model 2655 00 Flame Photometer Maintenance continued 7 8 Cleaning the Optical Filters and Glass Chimney EQUIPMENT REQUIRED Soft lint free cloth Methanol Cotton buds Tissues Cleaning Solution l Check that the flame on LED is off fuel is turned off at source and the flame photometer and compressor are switched off WARNING Do not proceed until all the parts within the chimney are at a safe handling temperature Lift off the chimney cap from the chimney assembly Lift out the glass chimney Move the filter selector to the top position and push the lever upwards and to the right This will disengage the filter selector from the stop Lift the filter selector out of the chimney Position the filter selector on the lint free cloth so that the coloured sides of the filters are uppermost and the thumb grip is at the top as shown in Figure 7 2 CAUTION Handle the optical filters only by the edges never the faces Using a soft probe cotton bud or similar that will not scratch the filters push out the first filter Examine the filter edges and note the wavelength on Figure 7 2 alongside the appropriate space Remove the remaining two filters a
20. e Photometer Troubleshooting continued 8 3 8 4 8 5 Unable to set display to zero Check that diluent is being aspirated The deionised water supply may be contaminated use a better grade of deionised water The burner may be encrusted with salt deposits Clean the burner as detailed in Section Tet If using a chart recorder check that the data output connections are correct If fault persists contact your Cole Parmer Distributor Unable to set display to standard reading Check the concentration of the standard being aspirated and that the dilution ratio of this standard is correct Use fresh deionised water and recalibrate zero If using low grade deionised water the background levels may be too high Make sure the correct filter has been selected If there is a chance that the optical filters may have been serviced check that they have been fitted correctly refer to Section 7 8 The nebuliser may be blocked Check the nebuliser refer to Section 7 6 Check the glass chimney is clean refer to Section 7 8 Make sure the fuel control is set correctly refer to Section 5 3 If the atmosphere surrounding the instrument or air compressor inlet is contaminated e g smoke incorrect readings may result If fault persists contact your Cole Parmer Distributor Unstable results Do not use compressed air from a communal source or Air Line as this may be contaminated with oil which will ruin the performance of
21. ecorder plug black 1 001 42 254 Recorder plug red 1 989 05 137 Sodium filter 589 6nm 1 989 05 138 Potassium filter 768nm 1 989 05 139 Lithium filter 671nm 1 400 16 004 Calcium filter 620nm with label 1 400 16 005 Barium filter 520nm with label 1 410 21 001 Filter holder recommended for use with Ca and Ba filters 1 001 08 234 Propane primary regulator cylinder fixing 1 001 08 733 Butane primary regulator 3080 for 15lb Calor Caravan cylinder 1 Spares and Accessories continued 9 3 Model 2655 00 Flame Photometer CAT NO ITEM QUANTITY 001 08 439 Butane primary regulator 182H for 32lb Calor Caravan cylinder 1 001 08 732 Butane primary regulator for Gaz type cylinder 1 001 72 114 Fuel tubing per metre 001 72 022 Air tubing per metre 001 08 718 Unex tubing clamp 1 100 99 010 Nebuliser cleaning wire pack of 3 1 pack 851 01 000 Model 851 Air Compressor 1 855 01 000 Model 855 Air Compressor complete with water separator 1 410 89 001 Service Manual 1 475 41 100 Model 805 Dilutor 1 471 71 900 Mains Supply Lead 1 001 26 074 Disposable sample cups 1ml pack of 1000 1 pack 001 26 033 Disposable sample cups 20ml pack of 50 1 pack 001 92 492 Guide to Flame Photometry 1 Standard Solutions NOTE The following solutions are available in 500 ml bottles CAT NO DESCRIPTION QUANTITY 001 56 120 Flame Photometer Standard 1000ppm Na 1 bottle 001 56 121 Flame Photometer Standard 1000ppm K 1 bottle 001 56 122 Flame Pho
22. esults on a graph against standard nanananteatinn an linane eranh nanan Dafarn ta awamnla ahar im Dinmmen 2 Model 2655 00 Flame Photometer Operating Instructions continued 5 4 Operating Instructions continued Ww 2 ad lt gt gt lt ssk a n ra a 18 15 SODIUM CONCENTRATION PPM Figure 5 3 Typical Calibration Curve 14 15 16 17 Check calibration standards and blank readings Dilute the unknown solutions with diluent to give a concentration of the element under test within the range of the calibration standards Several attempts might be necessary to determine the correct dilution ratio Aspirate each of the diluted unknowns for 20 seconds then note the reading The concentration of the element in the unknown sample can be calculated by reading the sample concentration from the calibration curve and multiplying it by the dilution factor Recalibrate the instrument by carrying out paragraphs 8 to 12 Experience in use will determine how frequently the calibration needs to be checked Model 2655 00 Flame Photometer Operating Instructions continued 5 5 Shutdown Procedure 5 6 l Aspirate Cleaning Solution diluted 1 in 100 with deionised water for one minute 2 3 Aspirate diluent for two minutes For a short term shutdown two hours switch off the instrument power switch and switch off the compressor For a longer term shutdown overnight turn off the fuel supply at so
23. h of reinforced Nylon hose supplied between the air compressor outlet and the air inlet connector on the rear panel Secure both ends with Unex tubing clamps supplied Model 2655 00 Flame Photometer Installation continued 2 4 Assembly continued 17 Fit one of the 200mm lengths of drain tubing item 4 Figure 2 4 to the outlet on the constant head drain or Gas Trap If necessary connect a suitable length of tubing not supplied to extend the drain tubing to carry waste to a sink or other drain receptacle The downward flow of waste must not be restricted The second length of tubing supplied is a spare for either the U tube or drain tube Figure 2 4 Filling the U Tube 1 Wash bottle 2 Constant head drain Gas Trap 3 U tube 4 Drain tube 5 Spring clip 18 Make sure that the constant head drain Gas Trap is positioned so that the lip at the top is resting on the spring clip Use a wash bottle item 1 Figure 2 4 to fill the U tube with deionised water Sufficient water should be used to purge the tube of air Allow excess water to flow back into the drain IMPORTANT Do not continue until you are satisfied that the U tube has been completely filled with water and is purged of air Model 2655 00 Flame Photometer Installation continued 2 4 Assembly continued 19 20 21 22 23 The instrument is supplied with Na K and Li filters fitted To replace any of these fi
24. he ndash ceakootand SEN KUNRAN R NS ENT 9 PEC A T E 9 2 4 AI h A E EEE E E E 9 2 5 Connecting a Chart Recordi o csisascscsdsacss anassassdaateas seden sd Kr 15 Principles of Operaatiot ieira i a E A E a E aaa 16 3 1 Flame PROMETE until T 16 Performance Characteristics and SpecificatiOW ococononcncnnncnnnnnnnnnnnononononnnnnnnnnonononononononononononos 17 4 1 A O etisk AEEA E Barda Sirae E T de k en BO SR A be 17 4 2 IAS USE RAR aii di RR 17 4 3 PRUE SA ud enes eu dere A A Wonsdadadsnua di Son NR BENA 17 4 4 E RNE RONNE S 17 PAC AEG E a E E 17 Jean sin ses vai A deb E E E E E E ee bs 17 Reproduce MDA eainiie aa a i A TATAE 18 4 5 a ROO 18 4 6 A rocna aaan a A A RR Ner SNR R R ben RR ARN NA 18 O O RAA eyes 18 Method of eR OULD ecen a NS NN 18 SLINE O 18 4 7 Chart Recorder Qt put 3 c c1cccccdeiccsdcosecccdcctecesiicesesscucetacd FRE NS HORNE NRA RNA 18 4 8 Environmental Conditi ns tai te vans ra E SR SNR RENAR Nee Hr ER 19 MSRM NA 19 O dears NN 19 Tnstollation Cate pei ciedad 19 4 9 Power REQUIERES cian ice TE SRA SNARARE NAR RER KRANEN 19 UI ai 19 oo T E T E TSE ATS 19 PED E E E E A E A 19 E TUE E AA E A E a 19 ND NN 19 AL A E E E E E 19 w ae F NO Model 2655 00 Flame Photometer Contents Page Operas Vin CHIN iodo da id 20 51 Front Panel Controls and Indicators See Fig S 1 oocccncnnncnnononononononinnno
25. ing chamber with a slightly twisting action Lift the end cap to allow the water in the U tube to flow into the constant head drain Disconnect the U tube from the end cap and constant head drain Unclip the constant head drain and disconnect the drain tube Soak all the items removed from the instrument in a 1 in 100 dilution of Cleaning Solution agitating it periodically Rinse the parts thoroughly in deionised water and dry with clean tissues Fit the burner tube to the mixing chamber ensuring that the locating slot in the burner tube engages with the locating pin in the mixing chamber Fit the burner pointed end downwards into the burner tube Rotate the burner to ensure that it is fully inserted Check the condition of the end cap 0 ring seal If necessary replace the seal Replace the end cap using a twisting movement Align the hole with the mixing chamber screw fixing Fit the screw item 6 Figure 7 1 Carefully position the mixing chamber with the burner tube inside the chimney With the burner tube fully inserted twist the mixing chamber to the left through 40 degrees to lock it in position Refit the glass inner chimney and the chimney cover Fit the constant head drain to the spring clip and position it so that the lip on the drain is resting on the spring clip Connect the fuel tubing to the mixing chamber end cap Connect the U tube between the mixing chamber end cap and the constant head
26. ing to this particular product Such failure must have occurred because of a defect in material or workmanship and not have occurred as a result of operation of the product other than in accordance with procedures described in the instructions furnished with this product Conditions and specific exceptions which apply to the above statement are as follows 1 End user warranty time commences on the date of the delivery of product to end user premises Free of all charges statement applies only in areas recognised by Cole Parmer as being services either directly by its own personnel or indirectly through personnel of an authorized distributor Products purchased outside these areas requiring service during the warranty period will incur charges relative to the travel transit costs involved However products purchased in such areas will be serviced during the warranty period free of all charges providing they are returned carriage paid to either Cole Parmer or by pre arrangement to an authorized Cole Parmer distributor All maintenance other than operator maintenance as described in the instructions repairs or modifications have been made by Cole Parmer or Cole Parmer authorized personnel This product has where applicable been operated using Cole Parmer specified supplies and reagents Cole Parmer reserves the right to make any changes in the design or construction of future products of this type at any time without incurring an
27. irect special incidental or consequential damages whether based on contract tort or any other legal theory and whether advised of the possibility of such damages Neither Cole Parmer nor any of its third party suppliers makes any other warranty of any kind whether expressed or implied with respect to Cole Parmer products Cole Parmer Instrument Company 635 East Bunker Court Vernon Hills IL 60061 USA
28. lack plug Cat No 001 42 017 to the black ve recorder input lead and a red plug Cat No 001 42 254 to the red ve input lead Fit the plugs into the appropriate data output sockets on the rear panel of the instrument If the chart recorder has an input shorting link fitted connect it between the black negative input and the earth input This may reduce interference and produce a better trace Continue with Section 5 Operating Instructions 3 1 Model 2655 00 Flame Photometer Principles of Operation Flame Photometry When metal ions in solution are aspirated into a low temperature flame in an aerosol form the electrons of the ion are excited to higher energy states When these electrons return to the ground state they lose the excitation energy and a discrete wavelength of visible light is emitted This light wavelength can be isolated from other light wavelengths by an optical filter and the amount of light emitted can be detected with a suitable photodetector The amount of light emitted is proportional for low concentrations of metal ion only to the number of ions in the flame and hence the number of ions in solution The electrical signal from the photodetector is amplified and displayed on a digital readout 4 Model 2655 00 Flame Photometer Performance Characteristics and Specification 4 1 4 2 4 3 4 4 Readout 3_ digit light emitting diode LED display 12 5mm high Display range 0 to 199
29. lines Do not allow fuel to flow in the presence of unguarded flames e g cigarettes Always use genuine Cole Parmer replacement parts Do not for example replace the U tube with one of different material bore or length as this will cause a deterioration in the instrument s performance Always carry out the maintenance schedules as detailed in Section 7 Do not leave the inspection flap open unless adjusting flame conditions as this will allow stray light to enter the chimney The front panel of the Model 2655 00 is impervious to a wide range of chemicals However strong acids and some organic solvents e g chloroform and phenol may affect the finish Any spillage should be thoroughly wiped away as soon as possible If necessary clean the instrument with warm soapy water do not use abrasives 6 2 Hazards 1 All electrical instruments are potentially hazardous With the exception of the glass chimney there are no user maintainable parts inside the Model 2655 00 covers Never remove covers from the instrument unless specific maintenance instructions are being followed Propane Butane and natural gas are highly inflammable and potentially explosive gases Propane and Butane are stored as a liquid under pressure in a cylinder for use with the Model 2655 00 Such a cylinder should never be subjected to heat or mechanical shock When handled correctly and connected to the instrument as instructed the fuel gas is quite
30. lters refer to Section 7 8 Figure 2 5 Voltage Selector 1 Voltage selector 2 Fuse holders Check that the voltage shown on the voltage selector is correct for the local supply If it is not open the voltage selector compartment Slide the voltage selector bobbin item 1 Figure 2 5 out of the instrument and replace it so that the required voltage setting will be visible through the window of the compartment cover Close the compartment cover CAUTION Do not attempt to rotate the voltage selector bobbin when it is fitted in the voltage selector as this will damage the contacts Fit a suitable 3 pin plug to the a c supply lead Check that the correct colour coded leads are connected to the plug terminals Follow the plug manufacturer s fitting instructions CAUTION Ensure that the green yellow earth connector in the a c supply lead is connected to a properly grounded earth point the brown lead is connected to the Line terminal L and blue lead is connected to the Neutral terminal N If necessary set the power switch below the chimney to the 0 position Connect the a c supply lead to the power receptacle on the rear panel and connect the plug to a convenient supply socket If a chart recorder is to be used with the instrument continue with Section 2 5 If not continue with Section 5 Operating Instructions Model 2655 00 Flame Photometer Installation continued 2 5 Connecting a Chart Recorder 1 Connect a b
31. mm of bench space which includes an area in front for solutions and clearance at the rear for fuel and air tubing with clear access to the mains supply switch 3 Avoid sites that expose the instrument to direct sunlight or draughts 4 To meet the specification the ambient temperature must be within the range 10 C to 35 C and the maximum relative humidity must not be more than 85 non condensing 5 Make sure that the correct voltage is selected for the local a c supply as shown in the following table Do not attempt to connect the power supply lead until the voltage is correctly set VOLTAGE SELECTED VOLTAGE RANGE 100V 90V to 110V 120V 100V to 132V 220V 198V to 229V 240V 230V to 264V Model 2655 00 Flame Photometer Installation continued 2 3 Unpacking 2 4 1 Unpack the instrument and accessories NOTE The Model 2655 00 weighs 9 5kg follow safe lifting techniques 2 Check all items for damage 3 Check that all the items on the Accessory List have been delivered Contact your Cole Parmer distributor if you have any problems 4 The Model 2655 00 is shipped with the following items Accessory List CAT NO ITEM QUANTITY 001 72 043 Air tubing nylon reinforced 2 metres 001 72 114 Fuel tubing 2 metres 400 22 002 Drain tube 200 mm and spare tube 200mm 1 pack 100 594 Fuse 200 mA slo blo spares 2 471 71 900 Supply lead a c 1 100 99 010 Nebuliser cleaning wire pack of 3 1 pack 001 31 076 O ring
32. n the range 10 C to 35 C with a maximum of 7 C shift during 8 hours Installation Category Installation Category 1 Power Requirements Voltage 90V to 132V or 198V to 264V 50 60Hz Fuses Two 200mA slo blo fuses are fitted for all voltage ranges Power 20VA Fuel High grade Propane Butane or Propane Butane mixture All fuels to be free of heavy hydrocarbon deposits and regulated at the cylinder to approximately 2 1kg cm_ 30psig Natural Gas at 3 to 10 inches water gauge regulated to 2 inches water gauge using optional natural gas regulator NOTE Natural Gas may give reproducibility results outside specification Air A supply of clean air at a minimum pressure of 1kg cm_ 14psig at 6 litres minute as supplied by a Model 851 Air Compressor Maximum inlet pressure 2 1kg cm_ 30psig If condensation problems arise a Model 856 Air Compressor should be used which has a water separator fitted Size Overall including chimney and rear panel connectors 510mm high x 390mm wide x 345mm deep Wiainht 9 5 1 Model 2655 00 Flame Photometer Operating Instructions Front Panel Controls and Indicators See Fig 5 1 Power on LED which illuminates when the instrument is switched on Flame on LED which illuminates when the flame is alight Blank This control is used to set the display to zero while aspirating a blank solution Sensitivity Fine and Coarse These two controls are used to set the display t
33. nd mark the wavelengths on Figure 7 2 Carefully wipe each filter with a cotton bud soaked in methanol Turn the filter selector over so that the large holes and filter retainers are uppermost Refer to Figure 7 2 and carefully replace the filters mirrored sides upward into their respective positions Refit the filter selector into position inside the chimney and slide it fully in Check that all three positions can be selected Model 2655 00 Flame Photometer Maintenance continued 7 8 Cleaning the Optical Filters and Glass Chimney continued STANDARD FILTER 769 nm POSITIONS AS SUPPLIED Li 671nm Figure 7 2 Filter Selector 10 Soak the glass chimney in a 1 in 100 dilution of Cleaning Solution agitating it periodically 11 Rinse the glass chimney thoroughly in deionised water and dry with clean tissues 12 Replace the glass chimney with the clear strip at the bottom Make sure the glass chimney is seated correctly and refit the chimney cap on top of the chimney Model 2655 00 Flame Photometer Maintenance continued 7 9 Voltage Selection and Fuse Replacement EQUIPMENT REQUIRED Fuses WARNING For continued protection against fire hazard use only the same type and rating of fuse that was fitted originally to the M2655 00 refer to instrument rear panel l Check that the flame on LED is off fuel is turned off at source and the instrument and compressor are switched off 2 Disconnect the a c supply lea
34. nononoss 20 Pa RO 20 A E S 20 AN 20 Sensitivity Fine and Coise eiii 20 FAMED E 20 Pee STAT 20 A 20 Fonet A TIN 20 Fe Rear Panel Controls and Connectors nesnese reines kepi nns ejer vr vase ORO ON 23 Dala AINE ide 23 E esters ant Sea eo NER SERA fer E DR HN ETE 23 A A AS 23 RN 23 E A A 24 5 4 A o TO 25 5 5 OVA Procede a A ds 27 5 6 EPI FINNS A AAA 27 Operational Precautions and Limitations sssrinin iia RE E REEERE SR SN BENEN Te 28 6 1 Genea anoa e KINESER RNE DAN A AE E E EEE 28 6 2 JEEE A EA 28 A NO 29 real ROM Oil Gis ccc visedadactvs phaccunvauanearswadadeos suuecdvoupasuadanentevedadouavae baste E Quabbeacueraies 29 1 2 Daily NIE aida liado 29 13 Weekly Maintengt cota dada diia adan dada nr HAR sde ARN RNA 29 7 4 E 29 LS Six Monthly Maititemanice eninin a aai 30 7 6 INGE E e E E AE ANA SR SR RR 30 Operational Cheik inicien ende be b r s r SR adicto dais 30 Cleaning the eae is 30 asd Cleaning the Mixing Chamber Burner and DralM ooonnnnnncocicococncncnonononininininino 32 7 8 Cleaning the Optical Filters and Glass Chimney sesssstesstsrseovorsrsss ssorsrsi0i rr 33 Lg Voltage Selection and Fuse Replacement ooococoncccnnnnnnnnnnnnnnnnnnnnnnnnnnonnnnnnnnononos 35 7 10 Deprotemising or Disinfecting Procedure c00 c ccsccesccssavcsccessnesccssssceceosseeecenees 36 TEIDE A dt 37 8 1 Power on LED ot illuminated ecne naissen iaia 37 8 2 MS A EA 37 A O TON 37 DIO A E Ge ke ve 37 Puel GUPP occa dean coax
35. o an appropriate concentration reading while aspirating a standard solution The coarse control is a four position rotary switch and the fine control is a 10 turn potentiometer Fuel This control provides a fine adjustment of the fuel flow and enables the optimum flame conditions to be set for each element Na K Li This three position slider control selects the correct filter for the element to be determined When used with other filter types e g Ca or Ba the appropriate label should be fitted in the relevant position on the front of the chimney assembly Decimal This touch control located adjacent to the digital display is used to select the decimal point e g using the decimal pushbutton to select the decimal point a reading of 1000 will be displayed as 100 0 The sensitivity of the Model 2655 00 is unaffected by use of this control Power 0 1 Rocker switch that switches the a c power supply on 1 and off 0 When the instrument is switched on the power on LED is illuminated and an ignition cycle is initiated When switched off the flame is extinguished and the a c supply to the instrument is switched off Model 2655 00 Flame Photometer Operating Instructions continued 5 1 Front Panel Controls and Indicators continued Figure 5 1 Front Panel 1 Filter selector 2 Digital display 3 Inspection flap 4 Fuel adjustment control 5 Blank control 6 Coarse sensitivity control 7 Fine sensitivity control 8
36. r with deionised water and weigh it Present the 10ml beaker of deionised water to the nebuliser tube for an accurately timed minute Reweigh the beaker and calculate the aspiration rate If it is between 2 and 6 ml minute no further action is required If it is too low continue with paragraph 6 If it is too high contact your Cole Parmer Distributor Do not attempt to adjust the nebuliser as the capillary position is fixed during manufacture and is not adjustable Cleaning the Nebuliser 6 7 Release the nebuliser retainer item 1 Figure 7 1 and withdraw the nebuliser item 2 Figure 7 1 Insert a cleaning wire into the nebuliser capillary and pull right through Model 2655 00 Flame Photometer Maintenance continued Cleaning the Nebuliser continued Figure 7 1 Mixing Chamber 1 Nebuliser retainer 2 Nebuliser 3 End cap 4 Fuel tubing 5 Air tubing 6 End cap securing screw 7 U tube 8 Fit new nebuliser inlet tubing to the nebuliser Recheck the aspiration rate paragraphs 3 4 and 5 9 If nebuliser operation is still outside the specification unscrew the air line connector and remove the nebuliser inlet tubing 10 Soak the nebuliser in a 1 in 100 dilution of Cleaning Solution agitating it periodically 11 Rinse thoroughly in deionised water and shake dry 12 Refit the nebuliser inlet tubing and the air line Recheck aspiration rate 13 Ifthe nebuliser operation is still unsatisfactor
37. s or repairs are only carried out by persons authorized by them e The electrical installation of the relevant room complies with IEC requirements or the local regulatory code e The equipment is used in accordance with the instructions for use The information contained in this manual was correct at the time of going to print However Cole Parmer s policy is one of continuous product improvement and the right to change specifications equipment and maintenance procedures at any time without notice are reserved This manual is copyrighted and all rights are reserved No part of this manual may be reproduced by any means or in any form without prior consent in writing Cole Parmer Instrument Company 635 East Bunker Court Vernon Hills IL 60061 USA Tel 847 549 7600 Tol free 800 323 4340 Fax 847 247 2929 Model 2655 00 Flame Photometer Contents Page ended GG sot scone cacensncanockerenacosnccahoowshdneoennadatsnan ERA ad AAA A A Rada 2 NN 5 1 1 E ERNA R ARENA ER 5 1 2 SIMA Ol the eee eee 5 1 3 MB MN a ETONE P E A PE A OE ETS T 6 latinn aten R EET A SNR Sedhoaseulcemnadandteaeoaiaeetawets 6 TORA E NE E EE A A E EEEE E E E EEA T pA at O 7 A TAPDI Y orere i cee innate ns knarr ers EE OEE ER A ERE TOEN 7 Pd ii E E T a sheen eae 7 di deine det td e e a da a n N 7 FASO COME a ia A 7 2 2 SE CAI A da aida 8 2 3 MA cancesatendeatacadnodkatananea
38. st the blank control so that the display reads 0 0 Aspirate the highest concentration standard Allow 20 seconds for a stable reading and then adjust coarse and fine controls for a convenient reading e g 20ppm Na can be set to read 20 0 on the display Carefully adjust the fuel control for a maximum reading on the display ensuring that only small adjustments are made with a pause of several seconds between adjustments NOTE Ifyou have any difficulty obtaining a maximum sodium reading proceed as follows Open the inspection flap and adjust the fuel control until the flame just starts to lift off the burner Then turn the fuel control back counterclockwise until the cones of the flame are on the burner Close the flap and proceed with paragraph 11 Remove the standard solution wait 10 seconds then aspirate a blank solution of diluent for 20 seconds Adjust the blank control for a 0 0 reading Remove the blank solution and wait 10 seconds Repeat paragraphs 8 9 and 11 until the blank reading is 0 0 within 0 2 and the calibration reading is within 1 If a chart recorder is being used set zero on the blank solution and set span while aspirating the calibration standard Without touching the fine and coarse controls aspirate each of the remaining calibration standards for 20 seconds starting with the lowest concentration to avoid carry over again allowing 10 seconds between measurements Note the value of each standard and plot the r
39. the fuel supply is adequate Make sure the fuel control is set correctly refer Section 5 3 The nebuliser may be blocked Check the nebuliser refer Section 7 6 The U tube or drain tubing may be blocked or partially obstructed Use fresh deionised water and recalibrate zero If using low grade deionised water the background levels may be too high If fault persists contact your Cole Parmer Distributor 9 1 9 2 Model 2655 00 Flame Photometer Spares and Accessories Ordering Information When ordering spares or accessories for your instrument please give the following information to your Cole Parmer distributor 2655 00 Serial No Catalog No of Part Cat No Description Quantity required This will ensure that your order is dealt with quickly and efficiently The number shown in the third column Quantity is the quantity of items that are supplied against the stated Catalog Number If the quantity is greater than 1 then only multiples of that quantity can be supplied Spares and Accessories CAT NO ITEM QUANTITY 400 27 001 Nebuliser 1 410 26 001 Mixing chamber and burner 1 402 12 00 End Cap Assembly 1 400 02 013 End cap securing screw 1 410 04 010 Constant Head and Drain 1 100594 Fuse 200mA slo blo 1 400 22 003 Nebuliser inlet tube polythene 150mm each 1 400 20 001 Fuel restrictor 1 410 19 002 Natural gas regulator kit with filter 1 001 26 033 Sample pot plastic in packs of 50 1 pack 001 42 017 R
40. tometer Standard 1000ppm Li 1 bottle 001 56 123 Flame Photometer Standard 1000ppm Ca 1 bottle 001 56 124 Flame Photometer Standard 3000ppm Ba 1 bottle 001 56 184 Cleaning Solution 1 bottle 001 56 181 Diluent Concentrate 1 bottle 001 56 182 Tubing Disinfectant 500 ml 1 bottle 001 56 183 Deproteinising solution 80ml pack of 6 1 pack Model 2655 00 Flame Photometer Appendix A Bibliography Reference 1 Kolthoff I M Z Anal Chem 70 397 1927 2 Shohl A T and Bennett H B J Biol Chem 78 643 1928 3 Hald P M J Biol Chem 167 499 1947 4 Bernstein R E S Afr J Med Sci 17 101 1952 5 Alkemade C T J Smit J and Verschure J C M Biochimica et Biophysica Acta 8 562 1952 6 Collins G C and Polkinthorne H The Analyst 77 917 pp 430 436 1952 Ts Dryer R L Clin Chem 2 112 1956 8 Puffeles M and Nessim N E The Analyst 82 976 1957 9 Boling E A J Lab and Clin Med 63 501 1964 10 Amdisen A Scand J Clin and Lab Invest 20 2 104 1967 Model 2655 00 Flame Photometer Cole Parmer Instrument Company Warranty Statement Product Warranty Serial Number Warranty Term 12 Months Cole Parmer warrants subject to the conditions itemized within this document through either Cole Parmer personnel or personnel of its authorized distributors to repair or replace free of all charges including labour any part of this product which fails within the warranty time specified above appertain
41. urce When the flame on LED is extinguished switch off the power switch and the compressor This ensures that the fuel pressure in the fuel tubing is at a minimum Operating Hints 1 The deionised or high quality distilled water used when making a batch of diluent must be free from contaminating elements It is recommended that the same batch of diluent is used to prepare all solutions and to set blank on the instrument Greatest accuracy will be obtained by using the same dilution equipment for both standard and sample preparation Always use suitable standards for calibrating the instrument Remember that the accuracy of the results obtained from the Model 2655 00 depend on the accuracy and purity of the calibration standard that is used Applications and Method Sheets are available from your Cole Parmer distributor A guide to Flame Photometry is available from your Cole Parmer distributor Model 2655 00 Flame Photometer Operational Precautions and Limitations 6 1 General 1 Always dilute samples and standards with the same batch of diluent made up of 1 part Diluent Concentrate and 999 parts of deionised or good quality distilled water which contains non ionic wetting agents The samples should not be highly viscous or non homogeneous If possible samples likely to contain sediment should be filtered and then mixed to obtain a representative result Always use soap solution when checking for leaks in fuel or air
42. ure that the gas flow arrow on the regulator points to the right 10 Connect the Natural gas supply to the Natural gas regulator inlet with the fuel tubing and Unex clamp supplied with the instrument Model 2655 00 Flame Photometer Installation continued 2 4 Assembly continued 11 12 13 14 15 16 If it is necessary to fit the filter because of suspected dirty gas then this should be fitted before the Natural Gas regulator by cutting the rubber hose and fitting the filter in the tubing with the arrow pointing towards the regulator Secure the tubing to the filter and regulator with the extra Unex clips supplied with the Natural Gas regulator kit Connect the Natural gas outlet tubing supplied with the regulator to the gas inlet connector item 4 Figure 2 2 using the connector and nipple removed as described in paragraph 7 above bypassing the Propane Butane regulator Secure with Unex clamp supplied Figure 2 3 Natural Gas Regulator 1 Regulator screw cap 2 Filter in line with Rubber hose supplied with the instrument 3 Natural gas outlet tubing 4 Mounting bracket Remove the screw cap item 1 Figure 2 3 Rotate the screw head fully clockwise and then counterclockwise exactly one turn Refit screw cap Turn on the fuel supply and check all connectors for leaks using soap solution Do not use the instrument until you are satisfied that the installation is leakproof Connect the lengt
43. y fit a new nebuliser Do not attempt to adjust the nebuliser as the capillary position is fixed during manufacture and is not adjustable Model 2655 00 Flame Photometer Maintenance continued 7 7 Cleaning the Mixing Chamber Burner and Drain EQUIPMENT REQUIRED Flat blade screwdriver blade width 8 mm Deproteinising Solution Tissues l 10 11 12 13 14 15 16 17 18 19 20 Check that the flame on LED is off fuel is turned off at source and the instrument and compressor are switched off Release the nebuliser retainer and withdraw the nebuliser items 1 and 2 Figure 7 1 Disconnect the fuel tubing item 4 Figure 7 1 from the end cap connector item 3 Figure 7 1 using a twisting movement Do not pull the tubing as this will make it grip the connector more tightly WARNING Use a heat resistant glove when handling the burner and burner tube if the flame has been alight within the preceding 30 minutes Do not proceed until all the parts within the chimney are at a safe handling temperature Remove the chimney cover and lift out the glass chimney Disconnect the mixing chamber from the chimney assembly by twisting it to the right through 40 degrees Lower the mixing chamber and burner assembly Lift the burner tube from the mixing chamber and remove the burner Unscrew the end cap securing screw item 6 Figure 7 1 Grasp the end cap item 3 Figure 7 1 and remove it from the mix
44. y obligation to make any changes whatsoever to this particular product Reagents supplies consumables accessories and user maintenance items are not included in this warranty 7 Repairs or replacement of any part failing due to abnormal conditions including the following are excluded from this warranty a Flood lightning earthquake tornado hurricane or any other natural or man made disaster b Fire bombing armed conflict malicious mischief or sprinkler damage c Physical abuse misuse sabotage or electrical surge d Damage incurred in moving the product to another location Model 2655 00 Flame Photometer Warranty Statement continued 8 User agrees to permit Cole Parmer personnel or personnel of its authorized distributor to make changes in the product which do not affect results obtained but do improve product reliability Representations and warranties purporting to be on behalf of Cole Parmer made by any person including distributors and representatives of Cole Parmer which are inconsistent or in conflict with the terms of this warranty including but not limited to the limitations of the liability of Cole Parmer as set forth above shall not be binding upon Cole Parmer unless reduced to writing and approved by an officer of Cole Parmer Instrument Company Except for the obligations specifically set forth in this warranty statement in no event shall Cole Parmer Instrument Company be liable for any direct ind
Download Pdf Manuals
Related Search