HYDRASYS LC TECHNICAL MANUAL
Contents
1. sa A 89 203113 1031530 SDVLNOW 91 680 20016 t t ei SIN 1N3 S ECH de Gp GH 9IW 1N3A 5 SIM INA CONNECT SITUA reno SS OZ Diddy 12 10 12 10W FIM oun CONG UE erc 104 TCHEM KRS W HT LON LR IER rs MEANS ate E A 92 AA 0 UR A ER BEER Lee 93 AAA REX Locarno Je sam 94 952. Note The assembly consists of 1 resistor and 1 thermal switch 8 12 DRYER FAN see figure 12 section 12 8 13 8 14 Remove the staining destaining dryer assembly see 810 Unplug the fan connector J3 located on the staining dryer P C board Unscrew and remove the 4 screws 8 then the fan C7 is free GEL HOLDER ELECTROMAGNET see figure 12 section 12 Remove the rear cover see 81 the keypad and screen cover see 82 Unplug the electromagnet connector J5 located on the staining dryer P C board Unscrew with pliers the electromagnet C2 from the tank and remove it STAINING DESTAINING TANK see figure 12 section 12 Remove the staining destaining dryer assembly see 810 244 8 15 8 16 8 17 8 18 Unplug the level probe connector J4 and the electromagnet connector J5 located on the staining dryer P C board Unscrew and remove the screw used to fix the temperature transducer C11 Unscrew and remove the 4 screws C25 then the tank is free TANK LEVEL PROBE see figure 12 section 12 Remove the staining destaining tank see 510 514 Insert a blade flat screw driver between the tank and the level probe C3 edge to disengage it The leakproofness is made by the level probe O ring RADIATOR FAN see figure 4 section 12 Remove the rear cover see 81 Unscrew and remove the 4 screws M13 Remove the fan protection grid M12 Unscrew and remove the 4 screws 14 Unplug the
3. PELTIER MODULES see figure 5 section 12 Remove the migration module see 8 17 and put it upside down Unscrew and remove the 8 screws M16 Remove the radiator M10a be careful the temperature transducer T2 is fixed on it Remove the radiator M10b Then you have access to the 2 Peltier modules M5 Note The Peltier modules are connected in series be careful of their polarity 44 WARNINGS REASSEMBLE THE PELTIER MODULES 5 8 21 8 22 Apply a regular layer of thermal grease silicon compound on both sides Center the 2 Peltier modules with the migration plate respect the position given by the black and red wires Install the 2 radiators M10a and M10b Insert the 8 radiator screws M16 with the washers and tight them slowly alternating the screws with a torque screw driver set to 56 cNm centi Newton meter Check that the both Peltier modules remain centered on the migration plate and note that thermal grease escapes aroung module edges Tighten the 8 screws until the limit given by the torque screwdriver MIGRATION FAN see figure 5 section 12 Remove the migration module see 8 17 and put it upside down Unplug the fan connector J7 located on the cam motor P C board Remove the radiator M10a see 5 20 Unscrew the 2 screws M17 Remove the migration fan M7 with its holder THERMAL SWITCH AND TEMPERATURE PROBES 1 AND T2 see figure 5 section 12 Remove the migration module
4. qu Pt o d D 68 FIGURE 4 69 FIGURE 5 FIGURE 6 71 FIGURE 7 72 FIGURE 8 FIGURE 9 with the first model of gear pump 74 FIGURE 9 with the new magnetic drive gear pump FIGURE 10 76 FIGURE 11 77 FIGURE 12 78 FIGURE 13 SECTION 13 SCHEMATICS 80 K Vis 24242227 v i U im mans Pe A a ses Fosse rege d E Loes Fee meme veer Li 1555555 1 53 anu h A i 4 4 1 1 m Ae 72771 18 TE Ik H z E kERLL EE 31002091 3 6 E mm MAIN BOARD 85 232 Automat Gate array RS LLI 111 7713133 DESIGNATION anse ELI 5 CA Ee E BR vn 2 Il 3 2 H LR E GS m e m m m m m M re ES ES ass 31002091 MAIN BOARD D C Motors Fans Relays _ 84 NE LN 86 IN SE E E EA ie 79 38 m 38 SB i ch e Kran 87 C BOARD 2 Jnod 4 A LL Jnod ye 9 4 88 o d En rer FE
5. After each migration gently wipe the electrodes lengthwise and the migration plate with a wet tissue paper 9 2 WEEKLY 1 2 avoid accumulation of salt on the electrodes soak the migration carrier horizontally overnight in a bath filled to 5 mm height of distilled water Wipe with a dry tissue before putting it back in place To clean the staining tank proceed as follows reconstitute the washing solution as indicated in the instruction leaflet of the kit HYDRASYS WASH SOLUTION ref 4541 be sure that there is enough destaining solution 300 ml from the main menu press to have access to the staining program menu select TANK CLEANING program press START key green arrow on the right of the keypad The cleaning program consists of 3 steps one bath of washing solution connected to the line 6 of the valve washing solution 1 lt lasts 5 min with intermittent circulation of the solution When the cycle is finished the solution goes to the waste one bath of destaining solution connected to line 5 of the valve lt lasts 1 min and 40 sec with intermittent circulation of the solution When the cycle is finished the solution goes to the waste one drying step which lasts 8 min After drying the instrument emits a beep indicating that the cleaning program is finished and the gel holder is unlocked 9 3 YEARLY Replace the pinch valve and valve tubings lubricate the 4 O rings of the applicator
6. Main P C board faulty 56 EXPLANATION CORRECTIVE ACTION 61 Gear pump motor current Pump jammed dismantle the pump con sumption too high during body to clean the 2 gears draining of the tank with the Reagents and waste tubings pinched first model of pump SEIM Pinch valve and valve tubings pinched model Pump faulty Pinch valve or valve faulty Incorrect pump current calibration see section 5 1 4 Pump motor driver IC31 faulty Pinch valve driver IC28 faulty Main P C board faulty FAN REVOLUTION DETECTION 65 Migration chamber fan Fan stopped jam or mechanical problem fan too slow or no problem revolution pulses received Fan faulty Fan connector unplugged Power supply 24V faulty Motor driver IC 33 faulty Fan controller IC42 faulty Gate array automate 1 IC20 or gate array automate 2 1021 faulty Main P C board faulty Radiator fan problem fan too Fan stopped jam mechanical slow or no revolution pulses problem received Fan faulty Fan connector unplugged Power supply 24V faulty Motor driver IC 28 faulty Fan controller 1044 faulty Gate array automate 1 IC20 or gate array automate 2 1021 faulty Main P C board faulty 67 Staining module problem no Fan stopped jam mechanical revolution pulses received problem Fan faulty Fan connector unplugged Power supply 24V faulty Motor driver IC 33 faulty Fan controller IC45 faulty Gate array autom
7. P C board faulty TITLE EXPLANATION CORRECTIVE ACTION FILLING ERROR Chamber not filled in the programmed time EB or high level detected before low level Press start key to run a new cycle Jam Reagent container empty Reagent tubing pinched Pinch valve and valve tubings pinched Valve metal plate screws not tight Pump faulty Valve faulty Pinch valve faulty Pump motor driver IC31 faulty Pinch valve driver IC28 faulty Level probes faulty Main P C board faulty DRAINING ERROR Chamber not drained in the programmed Press start D key to run a new time cycle Jam Stain tubing or waste tubing pinched Pinch valve and valve tubings pinched Valve faulty Pinch valve faulty Pump faulty Pump motor driver IC31 faulty Pinch valve driver 28 faulty Level probes faulty Make a Reset History see 5 3 1 Main P C board faulty STAIN DRYER Temperature reached the D ERROR programmed time Press start key to run 1 or 2 cycles If incident remains select RESET HISTORY see section 7 from MEMORY and TESTS menus Press CONT to reset the temperature regulation parameters and execute a new staining cycle Temperature probe T3 faulty Check fuse F3 on staining dryer P C board Heating element thermal switch faulty Triac 2 opto isolator 2 located on the staining dryer P C board faulty Staining dryer P C board faulty Main P C board faulty 54 10 3 ERRORS These errors complete
8. SCREEN allows to select the 2nd screen 4 Jonorr ASPIRATION ON OFF pump filling under control of the operator to prevent any overflow of the tank ON OFF DRAINING ON OFF pump draining 21 PUMP SET UP allows to set up the pump speed value from 1 to 4 to drain or fill the tank 22 SECOND SCREEN LINE amp LEVEL HOME POSITION NEXT SCREEN FILL TANK WASTE TANK Ce PUMP CALIBRATION allows to select the line number and the tank filling level 1 intermediate level 2 high level Once the 2 parameters are validated the valve goes automatically to the chosen line allows to select the home position of the 6 or 10 way valve allows to select the 1st screen allows to fill the tank up to the defined level During filling pressing the key will stop the pump motor allows to drain the tank During draining pressing the key will stop the pump motor allows to calibrate only the first model of gear pump SEIM model This procedure must be performed after replacing the pump the main P C board or downloading of a new software It allows to calibrate automatically the maximum required pump current of the different cycles of the pump aspiration draining agitation Calibration parameters are stored in Internal Parameters parameters 45 to 49 Pump motor current control allows to stop the pump immediately to avoid disconnection of tubing in case of high pressure error 60 during filli
9. SECTION 7 MECHANICAL ADJUSTMENTS 31 SECTION 7 MECHANICAL ADJUSTMENTS 7 1 APPLICATOR ELECTRODE CARRIER To perform the adjustment proceed as follows turn the instrument on and let it finish its initialization select Service Program and enter the password 4644 press COMMANDS and APPLIC MOTOR Open the migration chamber cover and insert the side lateral carrier into the lateral slots The axis guides must slide into the slots without difficulty right up to the end If not pivot one of the axis guide according to the other make the carrier rise and fall with the 1 Joworr APPLIC command Observe the movement of the carrier it should be smooth and regular 7 1 1 APPLICATOR CARRIER ADJUSTMENT Insert 2 applicators in position 1 and 13 Put the carrier in the lowest position application position by pressing NEXT POSIT Adjust the screw front screw on the applicator arm underneath the cam so that the carrier is horizontal parallel to the migration plate and that the 2 applicators are distant of 1 5 to 2 mm from the carrier on both sides as shown on the next page 2303 APPLICATOR APPLICATOR CARRIER APPLICATOR CARRIER 1 5 TO2MM MIGRATION PLATE APPLICATOR MUST TOUCH Once the adjustment is made press NEXT POSIT make a complete cycle and check the 1 5 to 2 mm distance If correct block the lock screw with a flat wrench 5 mm Go to the intermediate position migrat
10. fan connector J2 located on the cam motor P C board Remove the radiator fan M11 MIGRATION MODULE see figure 3 section 12 Remove the rear cover see 81 the keypad and screen cover see 82 Unplug the high voltage connector located on the main power supply Unscrew and remove the 6 screws G19 2 migration module to the left to disengage it from the main board Lift the migration module G4 up to remove it MIGRATION COVER ELECTROMAGNET see figure 5 section 12 42 Remove the rear cover see 81 the keypad and screen cover see 82 Unscrew and remove the 2 screws 8 Unsolder the 2 wires and remove the electromagnet M2 with its holder 43 8 19 8 20 MOTOR AND MOTOR BOARD see figures 4 and 5 section 12 Remove the rear cover see 81 Unscrew and remove the 4 screws M13 Remove the fan protection grid M1 2 Unscrew and remove the 4 fan screws M14 to slide it to the back Unplug the connectors J1 J2 and J3 located on the cam motor P C board Open the migration cover M1 Unscrew and remove the 4 screws 9 Remove the cam motor with the P C board Unsolder the 4 wires of the motor from the P C board P1 P2 P3 and P4 Unscrew the cam screw Remove the cam Unscrew and remove the 2 screws M15 then the motor and the cam P C board are free The cam screw must be well tightened on the motor shaft flat to prevent any play between the motor and the cam
11. home position 6 Staining gel holder opto sensor 1 gel holder in the staining compartment 7 Migration cover microswitch 1 cover closed 8 Not used 5 1 3 Power supply commands TESTS V 0 1 0 R 0 00 1U 3 ON OFF P S U Allows to enter the voltage V Do not exceed 350V Allows to enter the current mA Do not exceed 200 mA ON OFF P S Allows to start and stop the power supply When the power is on the instrument continuously displays the voltage the current and the load values 20 Values when migration power supply is selected U Value in V Value in mA R Calculated value of the load in Notes It is not possible to modify U and when the power supply is on first stop the power supply and then modify the working conditions The power supply can only work if the migration cover is closed microswitch activated 5 1 4 Pump and valve commanas The accessible functions are spread on 2 screen pages Go from one screen to the other by pressing NEXT SCREEN FIRST SCREEN Firstline Values of the level probes NTC thermistors and position of the valve e LL Low Level Medium Level e HL High Level values of level probes without liquid 70 10 values of level probes with liquid 150 20 LINEN Position of the valve from 1 to 6 or 1 to 10 according to the valve VALVE ON OFF ON OFF valve motor NEXT POSITION next position of the valve NEXT
12. is 10 2 mm In migration position with less than 8 mm the contact gel sponge electrode is perfect In drying position with more than 16 mm there is no contact between the gel and the sponge 35 SECTION 8 DISMANTLING 2365 SECTION 8 DISMANTLING This section describes the procedures to dismantle the instrument in order to check or to replace parts of the instrument Proceed in the opposite way to reinstall the instrument Refer to Section 12 for the drawings CAUTION To avoid electrical shock make sure the instrument is disconnected from the A C power source before being opened Required tools slotted head screw driver 4 mm Allen wrenchs 1 5mm 2mm 2 5mm 3mm Torx wrench Torx 10 Torque wrench set to 56 cNm pliers cutting pliers soldering iron dust brush 8 REAR COVER figure 3 section 12 Unscrew and remove the 3 screws G22 pull to the back and remove the rear cover G3 8 2 KEYPAD AND SCREEN COVER figures 3 8 and 9 section 12 Remove the rear cover see 1 Unscrew and remove the 2 front screws 816 Unscrew and remove the 2 rear screws used to fix the cover on the staining tank Lift slightly the cover A1 unplug the connector J10 located on the main P C board 8 3 KEYPAD figure 8 section 12 Remove the rear cover see 1 the keypad and screen cover see 2 37 Unplug the keypad connector J2 located on the screen keyp
13. part of the screen corresponding to the module affected The module will then be put in the pause mode Pressing the corresponding green start D key restarts the interrupted cycle TITLE EXPLANATION CORRECTIVE ACTION MIGR MODE ERROR STAIN MODE ERROR APPLICATOR ERROR P S ERROR MIGRAT DRYER ERROR VALVE ERROR Migration module program error Staining module program error Position of the applicator electrode carrier cam not reached in the programmed time Migration power supply serial link error Migration plate temperature not reached in the programmed time during the drying cycle Valve position not reached the programmed time 54 Software problem Main P C board faulty Software problem Main P C board faulty Cam screw not tightened on the motor shaft Position opto 1 or 2 faulty Motor gear box faulty Motor driver IC38 faulty Main P C board faulty Serial link connector unplug on the main power supply ACIA IC22 or circuit IC23 faulty Main power supply faulty Main P C board faulty Power supply 30V faulty Temperature probe T1 faulty Thermal switch located beside temperature probe T1 faulty Heating element broken Relay K1 or K2 faulty 28 faulty Main P C board faulty the Valve coupling screws loose on the motor shaft flat Valve origin position opto faulty Motor gear box faulty Motor driver IC41 faulty Gate array Automate 2 IC21 faulty Main
14. 21015019 31001724 31001725 31001781 31001783 31001808 31001809 31001817 31002078 31002086 31002087 40000326 40000356 42589005 42589006 45360015 45360016 45360024 45400031 45640013 45750006 49600071 50000022 51000010 2 FRONT ELECTRODE CARRIER ASSY G10 POWER SUPPLY CAM MOTOR P C BOARD M4 M6 M21 MIGRATION HEATING ASSY VALVE ASSY 6 LINES V5 VALVE MOTOR P C BOARD VALVE ASSY 10 LINES V18 TUBING KIT FOR 6 AND 10 WAY VALVES G12 MAGNETIC DRIVE GEAR PUMP C20 STAINING DRYER P C BOARD G11 MAIN P C BOARD RELAY 24V 5A MIGRATION COMPARTMENT PLASTIC BODY M1 MIGRATION COMPARTMENT COVER M25 MIGRATION PROTECTIVE FILM P16 P19 SPONGE S PIN LEFT HANDLE OF THE STAINING GEL HOLDER RIGHT HANDLE OF THE STAINING GEL HOLDER G5 GEL POSITIONING GUIDE G5 KEYPAD V12 6 WAY VALVE INLET V13 6 WAY VALVE COMMON POINT CONTAINER TUBING 3 X 6 1M M5 PELTIER ELEMENT C13 HEATING ELEMENT 230V STAINING C13 HEATING ELEMENT 115V STAINING M2 MIGRATION COVER ELECTROMAGNET C2 GEL HOLDER ELECTROMAGNET B4 PINCH VALVE A6 DISPLAY OPTO SENSOR OPB 87L55 TRIAC TIC 253D TEMPERATURE PROBE AD593CN M26 V7 CAM VALVE MOTOR GEAR BOX M11 RADIATOR FAN 264 51000011 7 51000012 C7 STAINING DRYER FAN 262 63 SECTION 12 FIGURES FIGURE 1 64 FIGURE 2 65 FIGURE 3 with the first model of gear pump 66 FIGURE 3 with the new magnetic drive gear pump 67 1 E A o A
15. 5 5 2 1 This test indicates the pressed key To exit the keypad test press the key for few seconds 5 2 2 Display This test starts a graphic test followed by an alphanumeric test To exit the test press key 5 2 3 PC Serial port This test allows to check the RS232C serial port used to download the instrument It be started after having placed a jumper between pin 2 RX and pin 3 TX as shown below on the 9 pin connector AUX 1 AUX 1 REAR VIEW The decimal ASCII code of the pressed key sent to the transmission output TX and received through the loop by the reception input RX is displayed as follows PC SERIAL PORT Press a key TRANSMITTED RECEIVED ERROR 49 1 49 To exit the test press the key 26 This test allows to check the 6850 24 and the 2 input output adaptors 1488 IC 25 and 1489 IC 26 on the mother P C board 5 3 MEMORY MEMORY CHECK 1 RESET HISTORY 4 CHECK 8 VERSION 2 FLASH PROG 5 PARAM INTERNAL PARAM Test program used to reset the history to download a new software to check the memory and modify or reinitialize the internal parameters Note Memory consists of a Flash MEMORY and a RAM The Flash MEMORY 5 used to store the instrument software and the different migration and staining programs The Flash MEMORY is also used to store the internal parameters the heater temper
16. HYDRASYS LC TECHNICAL MANUAL HYDRASYS Version 1 0 Le 9 Novembre 1993 June 99 SECTION 1 SECTION 2 SECTION 3 SECTION 4 SECTION 5 SECTION 6 SECTION 7 SECTION 8 SECTION 9 SECTION 10 SECTION 11 SECTION 12 SECTION 13 SUMMARY 7 WARNING E 1 INTRODUCTION 2 ee 4 TECHNICAL SPECIFICATIONS 6 GENERAL DESCRIPTION c ai o a 8 SERVICE PROGRAM WE 14 SPECIAL FUNCTIONS E 25 MECHANICAL ADJUSTMENTS 27 DISMANTLING echan na edd cu kno as 32 MAINTENANCE lt lt ee seins 42 MESSAGES INCIDENTS AND ERRORS 44 SPARE PARTS ee 52 eler e 55 SCHEMATICS erregen 71 SECTION 1 WARNING SECTION 1 WARNING AND SAFETY NOTES Follow all warnings and instructions marked on or supplied with the instrument This service manual includes all information and warnings necessary for reliable and safe operation and maintenance If the instrument is used in any other way than that specified by the manufacturer operator s safety may be compromised The instrument has been designed to be used indoors lt can temporarily be subjected to temperatures up to 50 C and down to 10 C e g during transportation and storage without any adverse effects on its safe use Do not use the instrument near water or in wet locations Do not place the instrument on an unstable
17. HYRYS densitometer 4 2 INTERNAL HARDWARE DESCRIPTION see figures 1 to 13 section 10 It consists of on the left migration cover open migration plate coated by a Teflon film 2 electrodes anode on the left and cathode on the right a motorised cam used to move the applicator electrode carriers migration chamber fan M7 a migration cover microswitch M3 10 a safety electromagnet to lock the migration cover M2 11 in the middle rear cover and screen keypad cover removed Main board G11 on the right rear cover and screen keypad cover removed Teflon coated molded aluminium staining tank C1 3 level probes an electromagnet to lock the gel holder C2 astaining dryer board C20 a heating element 13 a fan C7 with an airflow convector C4 and C5 a pinch valve B4 a gear pump G12 an 6 or 10 way valve G7 with a valve P C board V5 at the back rear cover removed a power supply module G10 4 3 KEYPAD DESCRIPTION see figure 3 section 4 12 THE INSTRUMENT S FRONT PANEL Fig 1 N puni s ES M 3 1 Module migration Migration module Rep 4 Module coloration Staining module Rep 2 Ecran Screen Rep 5 Clavier keypad Rep 3 Porte Film Gel holder Rep 6 Vanne Valve 13 THE INSTRUMENT S REAR PANEL Fig 2 Rep 1 Compartiment F
18. ad interface P C board Insert slightly a small blade at the bottom left corner of the adhesive keypad G5 to unstick and remove it totally 8 4 FLUORESCENT SCREEN figure 8 section 12 Remove the rear cover see 1 the keypad and screen cover see 2 Unplug connectors J2 and J3 located on the screen keypad interface P C board Unscrew and remove the 4 screws A19 to separate the module from the cover Unscrew and remove the 4 screws A10 situated on the screen keypad interface board Unsolder the 2 wires Black Red of the screen from the keypad interface board VLCD 0V5 then the screen is free 8 5 MAIN P C BOARD figure 3 section 12 Remove the rear cover see 1 the keypad and screen cover see 2 Unplug connectors J2 J3 J5 J8 and J9 located on the main board Unplug the flat cable connected to the power supply cable J7 on the main P C board Unscrew the 2 screws G17 Slide the main P C board slowly to the right to unplug it from the migration compartment Remove the main P C board 8 6 MAIN POWER SUPPLY figure 3 section 12 Remove the rear cover see 1 Unplug all connectors located on the front of the main power supply and the connector located at the bottom right corner of the main power supply 38 Unscrew remove the screw used to fix the earth wire green yellow of the main power supply on the chassis Unscrew and remove the 5 screw
19. ate 1 IC20 or gate array automate 2 1021 faulty Main P C board faulty 57 EXPLANATION CORRECTIVE ACTION MISCELLANEOUS Interruption problem FIRQ Switch off the instrument for 40 s and restart Main P C board faulty Interruption problem IRQ Switch off the instrument for 40 s and restart Software problem Main P C board faulty 58 SECTION 11 SPARE PARTS 59 SECTION 11 SPARE PARTS REFERENCE 1270 10037021 10037022 10037023 10037024 10037025 10037026 10037027 10037031 10037032 10037033 10037034 10037035 10037036 10037110 10037111 10037112 10037120 10037210 10037220 10037221 10037250 10037514 10037563 10037566 10037567 10037800 10037810 DRAWING G6 G6 M23 11 C3 1 DESIGNATION HUMIDITY CHAMBER MASK POSITIONNING AXIS 2 IF MASK 4 IF MASK 2 BJ MASK 12 IF PENTA MASK 4 BJ MASK CSF MASK 15 ENZYMES MASK 30 ENZYMES MASK MASK R1 MASK R2 MASK R3 MASK R4 STAINING GEL HOLDER RIGHT AXIS FOR STAINING GEL HOLDER LEFT AXIS FOR STAINING GEL HOLDER STAINING GEL HOLDER FOR HYDRAGEL 7 CONTAINER 5 L CONTAINER 0 5 L BLACK CAP CONTAINER 0 5 L RED CAP CONTAINER 2 L MIGRATION TEMP PROBE PC BOARD STAINING TEMP PROBE PC BOARD LEVEL PROBE STAINING TANK APPLICATOR ELECTRODE CARRIER REAR ELECTRODE CARRIER ASSY 60 10037820 10043400 10043511 10043513 10043540 10043541 10043543 10043545 10043550 10043562 10043700
20. ature regulation parameters the latest values of the 3 level probes and the staining cycle step in progress in case of power failure 5 3 1 RESET HISTORY This test allows to reset information stored in the Flash MEMORY such as the latest migration staining programs selected the heater regulation parameters the latest values of the 3 level probes and the staining cycle step in progress in case of power failure When this test has been performed the instrument runs automatically a draining cycle of the staining tank 27 5 3 2 FLASH PROG This test allows to download a new software version through the serial link with a PC or HYRYS densitometer When this test is selected and validated by pressing CONTINUE the instrument emits an intermittent beep and displays SHUTTING DOWN indicating that it is waiting for a new software downloading Note For more details to download a new software version refer to the FLASH INSTRUCTION MANUAL If the test is selected by error turn the instrument off to start from the beginning 5 3 3 INTERNAL PARAMETERS Do not modify except the next parameters according to the instrument configuration 0 with the first model of gear pump SEIM model 1 with the new magnetic drive gear pump MICROPUMP model P44 6 with the 6 way valve 10 with the 10 way valve P45to P49 pump calibration parameters with the first model of gear pump SEIM model parameters set
21. cart stand or table Otherwise personal injury or damage to the instrument might occur This instrument should be operated from the type of power source indicated on the instrument s label and in this instruction manual Before switching the instrument on check that the voltage setting corresponds to the main power source If you are unsure of the type of power available consult your local power company The instrument is equipped with a 3 wire grounding type plug The plug should only be connected to a grounded power outlet Never use a ground adapter plug to connect the instrument to a socket lacking a ground connection terminal Never interrupt ground leads or disconnect the ground terminal inside or outside the instrument Use only fuses with appropriate rating value and of specified type Make sure that the total amperage of all appliances plugged into the power outlet does not exceed the outlet protection If an extension cord is used with this instrument ensure that the total instrument amperage rating does not exceed the extension cord power rating Disconnect the instrument from any power source before opening for testing cleaning adjustments servicing or maintenance Do not remove any covers when the instrument is connected to the main power source as this may expose dangerous live terminals Never defeat interlock switches They are provided to prevent access to unsafe areas Any adjustment maintenance and servicing o
22. de of the the migration plate temperature Peltier modules measured value is not within the defined limits 5 C to 95 C Temperature probe 1 faulty Amp 169 or A D converter 8 faulty Main P C board faulty 51 Regulation problem with the Select COMMANDS from TESTS menu radiator temperature probe on the screen next to 2 is indicated T2 bottom side of the Peltier the radiator temperature of the Peltier modules modules The measured value is not within the defined limits 5 C to 95 C Temperature probe T2 faulty Amp IC9 or A D converter 8 faulty Main P C board faulty ERROR EXPLANATION CORRECTIVE ACTION 52 Regulation problem with the Select COMMANDS from TESTS menu staining dryer module on the screen next to T3 is indicated temperature probe T3 the dryer temperature The measured value is not within the defined limits 5 C to 95 C Temperature probe T3 faulty Amp IC9 or A D converter IC8 faulty Staining dryer P C board faulty Main P C board faulty MOTOR CURRENT DETECTION Gear pump motor current Pump jammed dismantle the pump con sumption too high during body to clean the 2 gears filling of the tank with the first Reagent tubings pinched model of pump SEIM Pinch valve and valve tubings pinched model Pump faulty Pinch valve or valve faulty Incorrect pump current calibration see section 5 1 4 Pump motor driver IC31 faulty Pinch valve driver IC28 faulty
23. e migration and staining modules Em Note PELT amp DRYER Peltier effect and dryer commands APPLIC MOTOR applicator electrode carrier motor commands POWER SUPPLY migration power supply commands PUMP amp VALVE pump and valve commands FAN amp ELEC MAGNET fan and electromagnet commands exit of tests When you exit by pressing the instrument is initialized If the pump and the valve have been used the staining chamber is automatically drained to the waste container Information displayed during the tests T2 T3 migration plate temperature top surface of the Peltier modules radiator temperature bottom surface of the Peltier modules staining dryer temperature analog input of the precise channel used to determine the temperature slope of the migration plate value from 110 to 180 offset of the precise channel value from 45 to 65 5 1 1 Peltier and dryer commands TESTS T1 27 T2 27 T3 27 OP 58 CP 153 1 ON OFF MI DRYER 3 ON OFF ST DRYER 2 ON OFF PELTIER 4 SETUP PARAM MI DRY 0 ST DRY 0 PELTIER 0 18 ON OFF DRYER ON OFF Migration Plate Dryer ON OFF PELTIER ON OFF Peltier Effect Ls J ON OFF ST DRYER ON OFF Staining Dryer Ca Jeer UP PARAM Allows to set up the working conditions for testing the Peltier effect and the 2 dryers Working conditions MIGRAT DRYER POWER migration dryer power MI DRY powe
24. electrode carrier and check its adjustment refer to section 7 49 Maintenance kit p n 10043545 50 SECTION 10 MESSAGES INCIDENTS AND ERRORS 51 SECTION 10 MESSAGES INCIDENTS AND ERRORS 10 1 MESSAGES The messages that can appear on the screen are listed below They ask either to stand by for example Plate cooling or to act for example Waste tank full TITLE CORRECTIVE ACTION PLATE HEATING Wait for rise in temperature of migration plate If too long check relays K1 and 2 IC28 30V power supply Peltier modules and heating element PLATE COOLING Wait for cooling of migration plate If too long check relays 1 2 28 30V power supply and Peltier modules ILLEGAL SELECTION Wrong selection IN OPERATION Message appears if a program is chosen while one is already running WASTE TANK FULL Empty the waste container CLOSE DOOR Message appears if the migration program is started with the door opened Close the door then start the migration procedure again OPEN CLOSE DOOR Open or close the door of the migration compartment NO GEL HOLDER If the staining program is started without first introducing the gel holder this message will appear on the screen Introduce the gel holder then start the staining procedure again _ 52 10 2 5 These incidents stop the activity of a module without interrupting the activity of the other one The incident appears in the
25. f uncovered instrument without disconnecting it from power outlet must only be made by qualified and trained personnel aware of the risks involved If it is believed that the instrument has been damaged and is unsafe avoid using it and contact Sebia s authorized representative Manufacturer s Responsibility Sebia or Sebia s authorized representative are responsible only for defects material and services if Adjustments alterations or servicing have been made by authorized personnel Electricity supply is in accordance with instrument specifications Instrument is used according to instructions The manufacturer is not responsible for damage from misuse abuse accident and disasters improper return shipping or adjustments alterations and servicing done by unauthorized personnel The characteristics of HYDRASYS LC are described in this manual SEBIA has the right to modify them without prior notice SECTION 2 INTRODUCTION SECTION 2 INTRODUCTION This totally new system has opened the way to automation of electrophoresis techniques on the Hydragel agarose gels HYDRASYS LC is a multiparameter semi automated instrument which performs any agarose gel electrophoresis that is available in the manual Hydragel series of tests e g Serum Proteins Immunofixation Lipoprotein Lp a Hemoglobins alkaline and acid Hemoglobin 1 Chol HDL The automated electrophoresis steps are performed in sequence sam
26. ion position by pressing NEXT POSIT and check that the applicator teeth are at least 4 mm above the migration plate Take out the applicators 7 1 2 ELECTRODE CARRIER ADJUSTMENT It consists of the rear electrode carrier anode adjustment Select the intermediate position migration position by pressing NEXT POSIT Adjust the screw of the electrode carrier rear screw underneath the cam so that there is more than 7 mm and less than 8 mm between the migration plate and the electrode The edge 7 mm thickness of the special tool gauge must pass under the electrode up to the 8 mm thickness Check the adjustment by making a complete cycle of the carrier 2335 Select the high position of the carrier home or drying position by pressing 2 Lous POSIT and pass the gauge between the rear electrode and the migration plate The gauge must pass easily more than 16 mm height If correct block the lock screw with a flat wrench 5 mm Press to exit Migration position Special tool gauge Rear electrode carrier E 8 mm 7 mm Migration plate Home position drying position Rear electrode carrier Special tool 16 mm Migration plate 34 Notes For both carriers perform the adjustments without any gel on the migration plate There is no adjusment for the front electrode carrier cathode Concerning the second adjustment electrode carrier the tolerance thickness of the sponges
27. l heating element 90 W STAINING DESTAINING DRYING Capacity for 40 staining programs Teflon coated molded aluminum compartment with 3 level detectors Entry exit of liquids controlled by a 6 or 10 way valve Liquids circulate through a self priming pump Drying by convection heater 600 W with laminar air flow GENERAL SPECIFICATIONS Keypad with 20 keys shift L C D 4 lines of 40 characters Safety lid locks on the migration and staining modules Test and control program Power 5 1000 Voltage 115 230 V 10 factory selected 50 60 Hz Fuses 230 V 2 x 6 3 A slow blow 115V 2x10 A slow blow Dimensions L 69 cm x W 52 x 25 L 27 2 in x W 20 5 in x 9 8 in Weight 30 kg 66 LB SECTION 4 GENERAL DESCRIPTION SECTION 4 GENERAL DESCRIPTION 4 EXTERNAL HARDWARE DESCRIPTION It consists of on the front panel see figure 1 section 4 migration module 1 a keypad with 20 keys SHIFT 5 aL C D with 4 lines of 40 characters 2 staining destaining drying module 4 on the right panel see figure 1 section 4 a 6 or 10 way valve 6 on the rear panel see figure 2 section 4 an ON OFF switch 3 an A C power source inlet with fuses compartment 1 2 a waste overflow sensor inlet 4 a 9 pin connector AUX allowing to download a new software in Flash EPROM through a serial link 5 with the help of a lap top computer or
28. ly all functions of HYDRASYS LC identification number is displayed on the screen Upon validation the instrument is reinitialized ERROR EXPLANATION CORRECTIVE ACTION LEVEL DETECTOR Low level detection problem Select COMMANDS from TESTS menu in the staining chamber on the screen next to LL it must be indicated 70 10 when the chamber is empty 150 20 when the chamber 5 full Low level probe faulty A D converter 8 faulty Amp IC34 current mirror Q6 diode D36 or transitor Q5 faulty Main P C board faulty Mid level detection problem in Select COMMANDS from TESTS menu the staining chamber on the screen next to ML it must be indicated 70 10 when the chamber is empty 150 20 when the chamber 5 full Middle level probe faulty A D converter 8 faulty Amp 1634 current mirror 010 diode D38 or transistor Q9 faulty Main P C board faulty High level detection problem Select COMMANDS from TESTS menu in the staining chamber on the screen next to it must be indicated 70 10 when the chamber is empty 150 20 when the chamber is full High level probe faulty A D converter IC8 faulty Amp IC35 current mirror Q8 diode D37 or transistor Q7 faulty Main P C board faulty 55 THERMAL REGULATION 50 Regulation problem with the Select COMMANDS from TESTS menu migration plate temperature on the screen next to T1 is indicated probe 1 top si
29. ng of the tank or error 61 during draining of the tank VALVE POSITIONNING IN PROGRESS TO LINE 5 OF THE VALVE CONNECT DESTAINING CONTAINER AND PRESS RETURN Please wait Filling in progress Please wait Measurement in progress Draining 23 The new magnetic drive gear pump MICROPUMP model does not need to be calibrated When using this pump the internal parameter setting should be the following see 5 3 3 P9 1 P45 200 P46 200 P47 200 P48 200 P49 200 5 1 5 Fan and electromagnet commands CE 0 0 0 0 SF 0 PV 0 1 COVER ELEC MAGNET 4 TANK ELEC MAGNET 2 MIGRATION FAN 5 STAINING FAN 3 PELTIER FAN 6 PINCH VALVE Six function keys are available to control fans electromagnets and pinch valve COVER ELEC MAGNET ON OFF migration cover electromagnet MIGRATION FAN ON OFF migration fan PELTIER FAN ON OFF radiator fan of the Peltier modules TANK ELEC MAGNET ON OFF tank electromagnet STAINING FAN ON OFF staining dryer fan PINCH VALVE ON OFF pinch valve of the fluidic Status of each element is displayed on the first line of the the screen 1 ON 0 OFF CE migration cover electromagnet MF migration fan PF radiator fan of the Peltier modules TE tank electromagnet SF staining dryer fan PV pinch valve 5 2 KEYPAD DISPLAY 24 gt SERVICE PROGRAM 1 KEYPAD SERIAL PORT 2 DISPLAY 2
30. oard Unsolder the 4 wires of the motor from the valve P C board P1 P2 P3 and P4 then the motor and the valve P C board are free The screws V21 must be well tightened on the motor shaft flat to prevent any play between the motor and the valve cam STAINING DESTAINING HEATER ASSEMBLY see figures 3 9 and 12 section 12 Remove the rear cover see 81 the keypad and screen cover see 82 Unplug all valve tubings Unscrew and remove the 2 screws B7 Remove pump cover B1 Unplug the pump tubing connected to the tank bottom fitting Unplug the top valve tubing connected to the tank top fitting Unplug the flat connector J8 located on the main P C board Unplug the connector J2 located on the staining dryer P C board 40 8 11 Untight the 2 screws of J6 unplug the 2 wires black wire connected to position 1 white wire connected to position 3 of J6 Unscrew and remove the screw C24 used to fix the earth wire C22 green yellow and the bottom left screw C25 used to fix the other earth wire Unscrew and remove the 4 screws G19 Remove the staining destaining dryer assembly HEATING ELEMENTS see figure 12 section 12 Remove the rear cover see 81 the keypad and screen cover see 82 Untight the 2 screws position 1 and 3 of J7 located on the staining dryer P C board and unplug the 2 wires Unscrew and remove the 2 screws C12 Pull up the heating elements C13 with the protection cover C14
31. ple application migration incubation staining destaining and drying The manual steps include handling the samples and gels and setting up the instrument for operation Sample application is performed in a particularly ingenious and original way The excellent quality of the applications allows the protein fractions to resolve into sharp and distinct bands Migration parameters are regulated in constant current constant voltage constant power or volts x hour Staining destaining and drying steps are performed in a leak proof compartment with a capacity of up to four different reagents The HYDRASYS LC control panel is connected to an L C D screen which can display menus the working stages of each module and the progress of the ongoing procedural step In addition it displays messages incidents and errors HYDRASYS LC should be used only with the specifically designed HYDRAGEL kits gels and reagents manufactured and tested by SEBIA SECTION 3 TECHNICAL SPECIFICATIONS SECTION 3 TECHNICAL SPECIFICATIONS SAMPLE APPLICATION Applicator 7 15 or 30 deposits Disposable microporous applicators Direct loading of 10 uL of biological fluid Automatic application controlled by the program MIGRATION Capacity for 40 migration programs Constant voltage current power or volts x hour Voltage 3 5 to 350 Current 3 5 to 200 mA Power 0 to 30 W Temperature controlled by Peltier effect Drying assisted by additiona
32. r applied to the heating element from 0 to 90 watts BATH DRYER POWER staining dryer power ST DRY power percentage conduction time of the triac applied to the heating element For safety reasons do not exceed 80 PELTIER VOLTAGE Peltier voltage PELTIER voltage applied to the Peltier modules from 0 to 30 volts MODE 0 COOLING functioning mode of the Peltier effect 0 cooling 1 heating Note By default the 3 parameters are programmed to 10 and the cooling mode is selected The sign for the Peltier effect indicates cooling the sign indicates heating WARNING Peltier effect and dryer commands are 3 ON OFF commands This means that no regulation is effective they are under control and responsability of the operator Never exceed 80 C for T1 T2 and T3 temperatures to prevent any damage 19 5 1 2 Applicator electrode carrier motor commands 3 function keys are available to control the cam motor the opto sensors and to adjust the applicator electrode carrier ON OFF APPLIC motor ON OFF HOME POSITION motor goes to the home position NEXT POSITION motor goes to the next position Port bit status the bits are numbered from 1 to 8 starting from the right 1 Application position opto sensor of the carrier 0 application position 2 Home position opto sensor of the carrier 0 home position 3 Not used 4 Waste container level 0 full 5 Valve home position opto sensor 1
33. s G18 Remove the main power supply G10 8 7 PUMP ASSEMBLY AND PINCH VALVE figure 9 section 12 Unplug all the valve tubing Remove the rear cover see 1 Unscrew and remove the 2 screws B7 Remove the pump cover B1 Unplug the pump connector J5 located on the main P C board Unscrew and remove the 2 screws B6 Unplug both pump tubings Remove the pump assembly G12 Remove the tubing from the pinch valve Unscrew and remove the screw used to fix the earth wire of the pinch valve at the bottom of the chassis Unplug the connector J2 located on the valve P C board Unscrew the 2 screws used to fix the pump holder B2 on the chassis Unscrew and remove the 2 screws B5 Remove the pinch valve B4 8 8 VALVE ASSEMBLY figures 3 and 9 section 12 Remove the rear cover see 1 Unplug all the valve tubings Unscrew and remove the 4 screws V15 Remove the valve metal plate V14 39 8 9 8 10 Unplug connector J2 located on valve motor board and the connector J2 located on the staining dryer P C board Unscrew and remove the 4 screws G21 Remove the mechanical part of the valve VALVE MOTOR AND VALVE P C BOARD figure 10 section 12 Remove the rear cover see 81 Remove the valve assembly see 88 Unscrew and remove the 2 screws V6 Untight the 2 screws V21 Unscrew and remove the 2 screws V8 Slide the motor to the right and remove it with the P C b
34. see 8 17 and put it upside down Remove the 2 radiators 10 and M10b see 520 Unsolder the 2 wires from the thermal switch M22 Unscrew and remove the screws of the thermal switch then it is free Unsolder the 2 wires from the temperature probe 1 Unscrew and remove the screw of the probe then it is free 45 For transducer T2 which is fixed radiator M10a the procedure is the same 46 WARNINGS 8 23 The hole where is inserted the temperature probe must be full of thermal grease silicon compouna Use epoxy glue on the temperature probe P C board to prevent any short circuit with water condensation between the legs of the transducer MIGRATION HEATING ASSEMBLY see figure 5 section 12 Remove the migration module see 8 17 Insert slightly a small blade between one of the corners of the adhesive migration film 25 and the migration surface to unstick and remove it completely Put the migration module upside down Remove the Peltier modules see 8 20 Unscrew and remove the 8 screws M18 Remove fixation parts M19 and M20 Remove the temperature probe T1 see 822 Unscrew and remove the 2 screws M24 used to fix the 2 earth wires green and yellow Unsolder the 2 wires of the heating element blue and Grey from the connector of the migration module then the migration heating assembly 5 free 47 SECTION 9 MAINTENANCE 48 SECTION 9 MAINTENANCE 9 1 DAILY
35. to 200 with the new magnetic drive gear pump MICROPUMP model as of software release 6 XX XX 5 3 4 MEMORY CHECKSUM AND VERSION This test allows to know the Flash memory checksum and the software version 5 3 5 REINIT PARAMETERS This test allows by pressing CONTINUE to reinitialize the internal parameters Then refer to 5 3 3 to set the internal parameters according to the instrument configuration 5 4 SELECT LANGUAGE This menu allows to select the user s language French English German Spanish Portuguese or Italian 28 SECTION 6 SPECIAL FUNCTIONS 29 SECTION 6 SPECIAL FUNCTIONS HIDDEN KEYS The special LJ key is necessary to activate the functions below After having pressed the key one must press the additional two keys simultaneously shortly after J maraton Allows to shorten the time of each migration cycle phase in process sranna Allows to shorten the time of each staining cycle phase process SHIFT Ce Allows to give access directly to the test program in case of error message then enter password 4644 SHIFT Resets the history and the instrument which starts up again and indicates INCORRECT STOP and thus drains the tank to the waste container Warning do not use this function when the tank is full of staining solution because stain will be drained to the waste instead of to the staining container 30
36. usibles Fuse compartment Rep 4 D tecteur de vidange Waste overflow inlet Rep 2 Entr e Cordon Secteur Power source inlet Rep 5 Port s rie RS 232 RS 232 serial port Rep 3 Marche Arr t ON OFF switch 14 MIGRATION STOP MIGRATION GREY KEY THE INSTRUMENT S KEYPAD AND SCREEN Fig 3 MIGRATION LED LCD SCREEN STAINING LED PROTEIN 15 30 PROTEIN SELECT MIGRATION 3 SELECT STAINING SERVICE PROGRAM 4 REAGENT LINES START STAINING STOP STAINING ENTER CURSOR KEYS 15 SECTION 5 SERVICE PROGRAM SECTION 5 SERVICE PROGRAM On the main menu select SERVICE PROGRAM and then a password 4644 must be entered When the Service Program is selected the following warning message appears on the screen WARNING Incorrect use may produce serious damage This program must only be run by authorized operators Press to continue SERVICE PROGRAM 1 COMMANDS MEMORY 2 KEYPAD DISPLAY 4 SELECT LANGUAGE TESTS T1 27 12 28 T3 25 68 145 1 COMMANDS 1 PELTIER amp DRYERI 4 PUMP 8 VALVE 2 APPLIC MOTOR 5 FAN 8 ELEC MAGNET 3 POWER SUPPLY SERVICE PROGRAM 2 KEYPAD DISPLAY 1 KEYPAD 3 PC SERIAL PORT 2 DISPAY MEMORY CHECK 1 RESET HISTORY 4 CHECK 8 VERSION 3 MEMORY T 5 REINIT PARAM 2 FLASH PROG 3 INTERNAL PARAM 17 5 1 COMMANDS Test program used to control the different elements of th
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