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Agilent 1200 Series Nano Pump

1. Analog signal to recorder canbuscalle NE EE AC power TI AC power Autosampler a Thermostat cable F P o J AC power Remote AC power cable Fo RE 1 AC power Analog signal to recorder Figure 4 Recommended stack configuration rear view 1200 Series Nano Pump Service Manual 23 1 Installing the Pump Installing the Nano Pump Preparations Locate bench space Provide power connections Unpack the pump Parts required Pump Parts from accessory kit see Nano Pump Accessory Kit Content G2226 68705 on page 17 Power cord for other cables see text below and Chapter 5 Identifying Cables ChemStattion and or Control module G1323A B WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 24 Ensure the line power switch at the front of the pump is off 1200 Series Nano Pump Service Manual Installing the Pump 1 Ins
2. Balls of spindle drive gt gt Pump head screws screws and the balls of the spindle drive 5 Reconnect the capillaries tubing and the active inlet valve cable to the connector AIV connector Adapter capillary Inlet tube 140 1200 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the Optional Interface Board CAUTION Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use an ESD protection when handling electronic boards and components When required Board defective Part required BCD Interface board see Optional Interface Boards on page 229 1 Switch off the nano pump at the main power switch Unplug the pump from main power Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the nano pump Install the new interface board Secure screws oF Ww N Reconnect the cables to the board connector BCD Interface board NG Figure 21 Exchanging the Interface Board 1200 Series Nano Pump Service Manual 141 3 Repairing the Pump Exchanging Internal Parts The following procedures require opening the main cover of the nano pump Always ensure the nano pump is disconnected from the line power when the main cover is removed The security lever at the power input socket prevents that the pump cover is taken off when line power is
3. 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Leak Test The leak test is a built in troubleshooting test designed to demonstrate the leak tightness of the nano pump The test should be used when problems with the nano pump are suspected The test involves monitoring the pressure profile as the nano pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness and operation of the nano pump components Description The pump outlet is blocked with a blank nut and then the test is run using isopropyl alcohol IPA while monitoring the pressure profile using an integrator on the analog output or in the plot screen in the Control Module or the ChemStation The pressure profile is shown in Figure 9 Pump B Pump ie t Pressure Pump A RIDI 2 Piston 1 Plateau 4 bar Piston 2 Plateau 2 Plateau 3 epee aa Ramp 3 Plateau 1 i Ramp 5 Ramp 6 250 4 200 4 Ramp 2 1504 100 4 504 i Ramp 1 T T T T T T T T 0 05 1 1 5 2 2 5 di 3 5 4 Time minutes Figure 9 Typical Leak Test Pressure Profile with IPA Ramp1 The test begins with the initialization of both pumps After initialization plungers Al and B1 are both at the top of their stroke Next the nano pump begins pumping solvent with a flow rate of 150 ul min stroke of 100 ul anda composition of 51 A 49 B Both pumps deliver for one complete pump cycle At the end of this ste
4. External contact 229 6 Introduction to the Nano Pump There are general purpose cables available to conenct BCD output see BCD Cables on page 200 and the external outputs see External Contact Cable on page 204 to external devices Table 32 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD 5 20 2 BCD 7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 14 not connected 15 5V LAN Communication Interface Board One board is required per Agilent 1200 stack It is recommended to add the LAN board to the detector with highest data rate 230 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 The LAN board can only be used together with a main board version G13XX 66520 for G1315A G1365A G1314A G1310A G1311A G1312A and G1313A or or newer and all other 1200 modules a DOS ChemStation software revision A 06 01 or above The following cards can be used with the Agilent 1200 modules Table 33 LAN Boards Type Vendor Supported Networks G1369A Agilent Technologies G1369 6001 Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX recommended for re ordering J4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J4100A Hewl
5. G1311 60002 5063 6586 5001 3739 G1312 67300 178 1200 Series Nano Pump Service Manual Parts and Materials 4 Figure 28 Pump Head Assembly 1200 Series Nano Pump Service Manual 179 4 Parts and Materials Electro Magnetic Proportional Valve EMPV Table 20 EMPV Assembly Item Description Part Number 1 EMPV complete assembly G1361 60000 2 Filter to EMPV capillary G1375 87400 3 EMPV to flow sensor capillary nanoflow sensor G1375 87321 4 Screw 0515 0850 Figure 29 EMPV Assembly 180 1200 Series Nano Pump Service Manual Flow Sensor Assembly Table 21 Flow Sensor Assembly Parts and Materials 4 Item Description Part Number 1 Nanoflow sensor assembly EMPV to flow sensor capillary nanoflow sensor FS to inj valve cap 350 mm 25 um for nanoflow sensor FS to inj valve cap 550 mm 25 um for nanoflow sensor G1376 68704 G1375 87321 G1375 87322 G1375 87323 Figure 30 Flow Sensor Assembly 1200 Series Nano Pump Service Manual 181 4 Parts and Materials Power and Status Light Pipes 182 Table 22 Power and Status Light Pipes Item Description Part Number 1 Light pipe power switch 5041 8382 2 Power switch coupler 5041 8383 3 Light pipe status lamp 5041 8384 4 Power switch button 5041 8381 Figure 31 Power and Status Light Pipes 1200 Series Nano Pump Service Manual Parts and Materials 4 Leak Parts Table 23 Leak
6. Jouy 13 2006 Date Z Agilent Technologies 1200 Series Nano Pump Service Manual 259 8 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Life Sciences amp Chemical Analysis Solutions under Support It will provide also the latest firmware of the Agilent 1200 series modules for download 260 1200 Series Nano Pump Service Manual Index A active inlet valve 120 121 active inlet valve drive 221 address switch 237 Agilent on internet 260 air flow 157 algae information 257 ambient non operating temperature 16 ambient operating temperature 16 analog output 216 246 analog signal output 233 APG remote connector 26 APG remote interface 234 ASIC application specific integrated circuit 221 assembling the main cover 166 AUTO mode 215 AUX output 26 battery 222 BCD board 141 229 BCD output 216 BCD LAN board 229 bench space 15 blank nut 81 86 block diagram 224 225 board connector 146 board layout 147 boards interface board BCD LAN 229 C cable CAN 26 GPIB 26 overview 190 CAN bus 216 CAN cable 26 CAN interface 233 ChemStation 26 cleaning the pump 118 compensation sensor open 46 compensation sensor short 47 composition precision 246 compressibility compensation 246 condensation 15 configuration swit
7. Troubleshooting and Test Functions Flow Sensor Accuracy Calibration Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Description The flow sensor accuracy calibration test is designed to calibrate the flow accuracy and to compensate the electronic offset of the nano flow sensor This test should be done if high flow accuracy is needed at flow rates lower than 500 nl min The calibration of the flow sensor is based in the linear relationship between the flow rate and pressure drop in a capillary The zero point is corrected and calibration factors are evaluated The test begins with the initialization of the pump After initialization the EMPV is opening to release the pressure No primary flow is delivered After 2 minutes the flow sensor offset and pressure offset are measured The primary flow is set to 500 ul min for 1 minute and the pressure drop over the inline filter is measured The pump is operating in the flow control mode and is delivering 2 ul min for approximately 3 minutes At the end of this step the pump is switched to pressure control mode The actual pressure is the initial pressure The pump is delivering solvent for 3 minutes at a pressure equal to the initial pressure divided by 2 At the end of this step the corresponding flow sensor response is measured The pump is delivering solvent for 3 minutes at a pressure equal to the initial pressure divided by 4 At the end of this step t
8. e Not ready condition in one of the instruments connected to the remote line Defective remote cable e Defective components in the instrument showing the not ready condition Suggested Actions V Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis V Exchange the remote cable V Check the instrument for defects refer to the instrument s reference documentation 1200 Series Nano Pump Service Manual 41 2 42 Troubleshooting and Test Functions Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable Causes e CAN cable disconnected Defective CAN cable Defective main board in another module Suggested Actions V Ensure all the CAN cables are connected correctly V Switch off the system Restart the system and determine which module or modules are not recognized by the system Y Ensure all CAN cables are installed correctly 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Leak A leak was detected in the nano pump The signals from the two temperature sensors leak sensor and board mounted temperature compensation s
9. 2 Remove the top covers and foam see Removing the Top Cover and Foam on page 143 3 Disconnectall connectors from the main board Connector Description Connector Description S1 Configuration switch J1 GPIB P11 AIV A J2 CAN connector P15 Leak sensor J3 CAN connector P16 Damper J4 RS232 P17 Fan J5 Remote P18 Interface board J6 Analog output P21 Encoder B P2 Power supply P20 AIV B P8 Motor B P22 Solvent Selection Valve P12 Motor A P9 EMPV P14 Encoder A P19 Flow sensor When removing connectors counterhold on connector J3 with one hand 1200 Series Nano Pump Service Manual Repairing the Pump 3 Figure 22 Board Layout of the NPM Board DEDECRED Ci O O a J4 J5 J6 S1 J1 J2 J3 P2 P8 P11 P12 n sl eae P9 P14 pa A 2225 ool P15 C P16 P17 AE ag dii agape P19 aaa o_o ee Ce 000 P21 P22 4 Remove the connector screws from the GPIB Remote and from the analog pressure out
10. 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Table 36 Remote Signal Distribution continued Pin Signal Description 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler RS 232C The RS 232C connector is used to control the instrument from a computer through RS 232C connection using the appropriate software This connector can be activated by the configuration switch module next to the GPIB connector The RS 232C is designed as DCE Data Communication Equipment with a 9 pin male SUB D type connector The pins are defined as follows Table 37 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 1200 Series Nano Pump Service Manual 235 6 Introduction to the Nano Pump Table 37 RS 232C Connection Table
11. Item Description Part Number 1 EMPV to FS cap 220 mm 25 pm for nanoflow sensor G1375 87321 2 Outlet ball valve to piston 2 capillary G1312 67300 3 FS to inj valve cap 350 mm 25 um for nanoflow sensor G1375 87322 FS to inj valve cap 550 mm 25 um for nanoflow sensor G1375 87323 4 Mixing capillary G1312 67302 5 Connection tube G1311 67304 6 Restriction capillary G1312 67304 7 Filter assembly includes frit 100 pl 5064 8273 Frit 0 5 pm 5022 2185 8 Filter to EMPV cap 280 mm 170 pm G1375 87400 9 Damper to filter capillary 130 mm 250 um 01090 87308 10 Solvent tube pack of 4 G1322 67300 Corrugated waste tube 120 cm re order 5 m 5062 2463 176 1200 Series Nano Pump Service Manual Parts and Materials 4 Figure 27 Nano pump hydraulic path 1200 Series Nano Pump Service Manual 177 4 Parts and Materials Pump Head Assembly Table 19 Pump Head Assembly Item Description Part Number Pumphead assembly included items marked with Outlet ball valve Screw lock Screw M5 60 mm Apdater Pump chamber housing Active inlet valve without cartridge Replacement cartridge for active inlet valve Seal pack of 2 Seal pack of 2 for normal phase applications Plunger housing including springs Sapphire plunger Support ring Outlet valve to piston 2 capillary G1311 60004 G1312 60012 5042 1303 0515 2118 G1312 23201 G1311 25200 G1312 60025 5062 8562 5063 6589 0905 1420
12. Ta e e n 0 MO Nut and screws a preg T A Yema o VAZZO He 6060 Taster Te oo TPB hae Baudrate Parity tee tee EB WOT woen e ii oerzee7s ANNE C sE c gt lt GLEE a Figure 23 Rear of Nano Pump 11 Replace foam and top covers see Replacing the Top Cover and Foam on page 167 12 Reinstall the pump in the stack Reconnect all cables Turn on the nano pump If the status indicator turns red continue with the section Open POINT 148 1200 Series Nano Pump Service Manual Repairing the Pump 3 Changing the Product Number and Serial Number When required If main board has been replaced Tools required User interface Parts required none Preparation Turn the module ON Start the user interface When the main board has to be replaced the new board does not have a serial number For some modules e g pumps or autosamplers the type has to be changed multiple usage boards Use the information from the serial number plate of your module The changes become active after the reboot of the module Using the Agilent ChemStation Module serial numbers are entered by typing specific commands on the command line at the bottom of the main user interface screen 1 To
13. The calibration of the flow sensor is based in the linear relationship between the flow rate and pressure drop in a capillary The zero point is corrected and calibration factors are evaluated Flow Sensor Accuracy Calibration on page 98 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Status Indicators Two status indicators are located on the front of the nano pump The lower left one indicates the power supply status the upper right one indicates the instrument status Status indicator A seccare ESA w E Power supply indicator Figure 7 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON When the indicator is off the module is turned off Otherwise check power connections availability of power or check functioning of the power supply 1200 Series Nano Pump Service Manual 35 2 Troubleshooting and Test Functions Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions When the status indicator is OFF and power switch light is on the nano pump is in a prerun condition and is ready to begin an analysis A green status indicator indicates the nano pump is performing an analysis run mode A yellow indicato
14. 154 fan 156 high pressure pump main board HPM board 146 interface board 141 internal parts 117 142 leak sensor 162 261 Index outlet ball valve 120 124 outlet ball valve sieve 120 124 pistons 120 137 power supply 160 pump drive 158 pump seals 120 134 purge valve 120 126 127 purge valve frit 120 126 127 solvent selection valve 130 status light pipe 165 wash seals 120 138 external contacts 216 F fan 156 fan drive 222 fan failed 48 features GLP 247 instrument layout 218 safety and maintenance 247 firmware description 227 from internet 260 main system 227 resident system 227 updates 228 first injection 29 flow connection diagram 28 flow range 246 flow sensor calibration 96 flow sensor parts 181 foam 143 167 foam parts 186 frequency range 16 216 frit 126 127 fuse 216 222 fuses BCD board 229 power supply 243 G GPIB default addresses 233 interface 233 GPIB cable 26 GPIB connector 216 262 hexagonal key 3mm 132 134 137 139 hexagonal key 4mm 132 134 137 139 158 160 high pressure pump main board HPM 221 holmium oxide declaration of conformity 259 HPM board 146 humidity 16 hydraulic path parts 176 hydraulic system 246 index adjustment 72 index limit 71 index missing 73 indicator power supply 35 information on uv radiation 256 initialization failed 75 injection first 29 inlet valve fuse 59 inl
15. G1375 87316 High pressure plug 5042 6486 Micro valve plug 5022 2187 Flow sensor accuracy calibration capillary 8000 mm 25 um G2226 67300 ESD wrist strap 9300 1408 Micro valve plug 5022 2187 High pressure plug 5042 6486 Seat capillary 150mm 0 075mm ID G1375 87316 18 1200 Series Nano Pump Service Manual Installing the Pump 1 Optimizing the Stack Configuration If your nano pump is part of a complete 1200 series system you can ensure optimum performance by limiting the configuration of the system stack to the following configuration This configuration optimizes the system flow path ensuring minimum delay volume If a single stack configuration becomes too high e g if an additional module like a G1330B ALS Thermostat is added or if your bench is too high a two stack configuration may be a better setup Separate the stack between pump and sampler and place the stack containing the pump on the right side of the stack containing the sampler 1200 Series Nano Pump Service Manual 19 1 Installing the Pump Solvent cabinet Vacuum degasser Pump Instant Pilot Well plate sampler Column compartment Figure 1 Recommended Stack Configuration Front View 20 1200 Series Nano Pump Service Manual Installin
16. Pump Head Missing Pump channel A B Pump Head Missing Pump channel B The pump head end stop in the nano pump was not found When the nano pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error message is generated Probable Causes Pump head not installed correctly screws not secured or pump head not seated correctly Broken plunger Suggested Actions V Install the pump head correctly Ensure nothing e g capillary is trapped between the pump head and body V Exchange the plunger 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Index Limit Index Limit Pump channel A B Index Limit Pump channel B The time required by the plunger to reach the encoder index position was too short nano pump During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable Causes e Irregular or sticking drive movement e Defective pump drive assembly Suggested Actions V Remove the pump head and examine the seals plungers and internal components for signs of wear contamination or damage Exchange components as req
17. gt be VA 220 Hz 60 60 Cc DC PE bis bia gt jane 1 2 j3 4 s elr 8 C DC DC gt C 2 gt 2 e ae ay o tte fera C c IESE CD LIL woen pa gt C DIC DG gt DEI GERMANY n AZ LI iu Cc gt Power Plug Security Lever Figure 39 Electrical Connections to the Nano Pump WARNING To disconnect the nano pump from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off 1200 Series Nano Pump Service Manual 217 6 Introduction to the Nano Pump Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the meta
18. 01018 60025 Waste tube 2 m 0890 1760 SST replacement frit 0 5 um 5022 2185 Wrench open end 7 16 1 2 inch x 2 8710 0806 Wrench open end 1 4 5 16 inch x1 8710 0510 Wrench open end 14 mm x 1 8710 1924 Wrench open end 4 mm x 1 8710 1534 Hex key 2 5 mm 15 cm long straight handle x 1 8710 2412 Hex key 3 0 mm 12 cm long x 1 8710 2411 Hex key 4 0 mm 15 cm long T handle x 1 8710 2392 Torque adapter G1315 45003 CAN cable 1 m long 5181 1519 Purge valve assembly G1311 60009 Purge valve holder G1312 23200 Screw for the purge valve holder 0515 0175 PFS capillary 350 mm 25 um G1375 87322 PFS capillary 550 mm 25 um G1375 87323 PFS capillary 150 mm 75 pm G1375 87316 High pressure plug 5042 6486 Micro valve plug 5022 2187 Flow sensor accuracy calibration capillary 8000 mm 25 um G2226 67300 ESD wrist strap 9300 1408 Micro valve plug 5022 2187 High pressure plug 5042 6486 Seat capillary 150mm 0 075mm ID G1375 87316 1200 Series Nano Pump Service Manual 187 4 Parts and Materials 188 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 5 Identifying Cables Cable Overview 190 Analog Cables 192 Remote Cables 195 BCD Cables 200 Auxiliary Cable 202 CAN Cable 203 External Contact Cable 204 RS 232 Cable Kit 205 LAN Cables 206 n i a Agilent Technologies 189 5 Identifying Cables Cable Overview Never use cables other than the ones supplied by Agilent Technolog
19. 147 6 Remove both pump assemblies For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the connector P8 P21 and P12 P14 at the main board 1200 Series Nano Pump Service Manual Repairing the Pump 3 7 Disconnect the fan cable P17 at the main board 8 Remove the solvent selection valve See exchanging the solvent selection valve in this chapter 9 Remove the CSM board Disconnect the remaining connectors see exchanging the high pressure pump main board in this chapter 10 Push the leak sensor cable through the recess of the solvent selection valve and lift out the bottom foam 11 Remove the power supply For this loosen and remove the power supply screws at the rear panel unclip the power supply light pipe from the power supply and pull out the coupler 12 Place the new power supply into the instrument and fix it with the two screws at the rear panel 13 Place the coupler onto the switch in the power supply and clip the light pipe back onto the coupler 14 Slide the leak sensor cable through the bottom foam and fix the leak sensor cable in the foam Make sure the leak sensor cable is not damaged by the sheet metal 15 Re install the CSM board Reconnect all the cables 16 Re install the solvent selection valve 17 Re install the two pump head Reconnect the capillaries and the cable 18 Re install the Z panel and the dampe
20. 4 DTR GND 5 ne 5 enD DSR 6 6 DSR RTS 7 7 RTS CTS 8 m 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male 1200 Series Nano Pump Service Manual 205 5 Identifying Cables LAN Cables 206 Recommended Cables Table 29 Description Part number Cross over network cable shielded 3 m long 5023 0203 for point to point connection Twisted pair network cable shielded 7 m long 5023 0202 for hub connections 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 6 Introduction to the Nano Pump Introduction to the Nano Pump 208 Electrical Connections 216 Instrument Layout 218 Early Maintenance Feedback EMF 219 The Electronics 221 Firmware Description 227 Optional Interface Boards 229 Interfaces 232 Setting the 8 bit Configuration Switch 237 The Main Power Supply Assembly 242 ig Agilent Technologies 207 6 Introduction to the Nano Pump Introduction to the Nano Pump 208 The nano pump consists of two identical pumping units in a single housing It generates gradients by high pressure mixing A solvent selection valve provides flexibility in the choice of solvents Mobile phase composition is produced by mixing the outputs of pump A and pump B The solvent selection valve allows the pump A output to originate from either channel A1 or channel A2 The pump B output may originate from either channel B1 or channel B2 The primary flow produced by the
21. Execute to initialize the leak test Once the test is started the nano pump increase the pressure and run each plunger at low flow rate The control module displays a graphical representation of the pressure in the plateau windows Evaluating the Results of the Leak Test on page 89 describes the evaluation and interpretation of the leak test results 6 When the test is finished slowly open the blank nut to release the pressure in the system 88 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Evaluating the Results of the Leak Test Defective or leaky components in the pump head lead to changes in the leak test pressure plot Typical failure modes are described below Pressure _ bar Plateau 3 Plateau 4 3004 Plateau 1 Plateau 2 2504 2004 1504 100 T T T T T D 0 5 1 15 2 25 a a6 4 Time minutes Figure 10 Leak Test Pressure Plot Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test where not within the specified limits Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened The press
22. If the temperature exceeds the upper limit of 95 C the error message is generated Probable Causes e High friction partial mechanical blockage in the pump drive assembly e Partial blockage of the flowpath in front of the damper e Defective drive assembly e Defective CSM board Suggested Actions V Ensure the capillaries and frits between the pump head and damper inlet are free from blockage V Ensure the outlet valve is not blocked V Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly V Exchange defective drive assembly V Exchange the CSM board 1200 Series Nano Pump Service Manual 61 2 62 Troubleshooting and Test Functions Motor Drive Power Motor Drive Power Pump channel A B Motor Drive Power Pump channel B The current drawn by the pump motor exceeded the maximum limit Blockages in the flowpath are usually detected by the pressure sensor in the damper which result in the nano pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper i e the pressure increase cannot be detected by the pressure sensor the nano pump will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the nano pump is switched off and the error message is generated Probable Causes Flow path blockage in front of the damper e Blocked outlet
23. Leak pan pump 5042 8590 6 Solvent selection valve half of a complete valve G1312 60000 Solvent selection valve screw 5022 2112 7 Pump drive assembly G1311 60001 Exchange pump drive assembly G1311 69001 8 Pump head see page 178 G1311 60004 9 EMPV holding screw 0515 0850 10 EMPV complete assembly valve and solenoid G1361 60000 11 AIV connecting cable G1311 61601 12 Damping unit 79835 60005 13 Fan assembly 3160 1017 1200 Series Nano Pump Service Manual 173 4 Parts and Materials Solvent Cabinet and Bottle Head Assembly Table 17 Solvent cabinet and bottle head assemblies Item Description Part Number Solvent cabinet assembly includes items 6 7 8 5065 9981 1 Solvent tubing 5 m 5062 2483 2 Tube screw pack of 10 5063 6599 3 Ferrules with lock ring pack of 10 5063 6598 4 Bottle amber 9301 1450 Bottle transparent 9301 1420 5 Solvent inlet filter SST 01018 60025 6 Leak pan solvent cabinet 5042 8567 7 Front panel solvent cabinet 5065 9954 8 Name plate Agilent 1200 5042 8901 Bottle head assembly includes items 1 2 3 5 G1311 60003 The assy comes with a glass solvent inlet filter Remove and replace it with the SST solvent inlet frit 01018 60025 174 1200 Series Nano Pump Service Manual Parts and Materials 4 Figure 26 Solvent cabinet and bottle head assembly 1200 Series Nano Pump Service Manual 175 4 Parts and Materials Nano Pump Hydraulic Path Table 18 Nano pump hydraulic path
24. Manual Troubleshooting and Test Functions 2 Testing your Nano Pump Micro Mode Pressure Test Step 1 Step 2 Description This is a fast test to verify the tightness of a micro system where the pump is operating in the micro mode and no manual purge valve is installed The flow path of the system which is tested for tightness is blocked by a blank nut The pressure is increased up to 380 bar and the remaining flow is measured in the flow sensor while the system is blocked The test begins with the initialization of both pump heads Next pump A begins pumping solvent until a system pressure of 380 bar is reached The pump is operating in the pressure control mode at 380 bar for several minutes The remaining flow in the column flow path between the EMPV and the blank nut is measured Running the Test 1 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Micro Mode Pressure Test 2 Start the test and follow the online instructions In step 10 of following procedure if you block the flow sensor outlet use the PEEK blank nut provided in the accessory kit Don t connect a SST blank nut to the flow sensor outlet this could damage the flow sensor 1200 Series Nano Pump Service Manual 77 2 Troubleshooting and Test Functions Micro Mode Pressure Test lel x Start Explain Close Result Status Nano Pump Pressure Tet_ __ Expected total time approx 10min __ I __ OE
25. P20 see Figure 22 on page 147 1200 Series Nano Pump Service Manual Repairing the Pump 3 10 Re install the mixing chamber in its holder 11 Re install the flow sensor Reconnect the capillaries and the cable 12 Re install the EMPV Reconnect the capillaries the waste tube and the cable 13 Reconnect both active inlet valve cables 14 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 167 1200 Series Nano Pump Service Manual 155 3 156 Repairing the Pump Exchanging the Fan Frequency Fan not running Tools required Screwdriver Pozidriv 1 Wrench 1 4 inch Parts required Fan PN 3160 1017 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 143 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 147 Remove
26. Parts Item Description Part Number 1 Holder leak funnel 5041 8389 2 Leak funnel 5041 8388 3 Tube clip 5041 8387 4 Leak plane pump 5042 8590 5 Leak sensor 5061 3356 Corrugated waste tube reorder pack 5m 5062 2463 Figure 32 Leak Parts 1200 Series Nano Pump Service Manual 183 4 Parts and Materials Cover Parts Table 24 Cover Parts Item Description Part Number 1 Plastic cover kit includes top base and both sides G1312 68713 2 Front plate G1376 68714 3 Logo plate Agilent 1200 5042 8901 Figure 33 Cover Parts 184 1200 Series Nano Pump Service Manual Parts and Materials 4 Sheet Metal Kit Table 25 Sheet Metal Kit Item Description Part Number 1 Sheet metal kit includes top base and Z panel G1376 68701 2 Screw for cover and Z Panel 5022 2112 3 Board cover 5001 3772 Included in item 1 Figure 34 Sheet Metal Kit 1200 Series Nano Pump Service Manual 185 4 Parts and Materials Foam Parts 186 Table 26 Foam Parts Item Description Part Number 1 Foam kit including upper and lower foam G1312 68702 2 Bushing for pump drive 1520 0404 3 Board guide 5041 8395 1 upper foam 1 lower foam Figure 35 Foam Parts 1200 Series Nano Pump Service Manual Parts and Materials 4 Nano Pump Accessory Kit G2226 68705 Table 27 Nano Pump Accessory Kit Content G2226 68705 Description Part Number Insert tool 01018 23702 SST Solvent inlet filter x4
27. Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1200 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector Pin Pin Signal Name Active 5061 3378 HP 1050 Agilent 1200 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 198 1200 Series Nano Pump Service Manual Identifying Cables 5 Agilent 1200 to HP 1090 LC or Signal Distribution Module Connector Pin Pin Signal Name Active 01046 60202 HP 1090 Agilent 1200 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 8 4 3 Gray Start Low 7 LA e 7 4 Blue Shut down Low uri 45 8 5 Pink Not connected 2 NI NC 6 Yellow Power on High 3 7 Red Ready High 5 Key 6 8 Green Stop Low NC 9 Black Start request Low Agilent 1200 to General Purpose Connector Pin Pin Signal Name Active 01046 60201 Universal Agilent 1200 TTL 1 White Digital ground i a i 2 Brown Prepare run Low au 3 Gray Start Low e ua a 4 Blue Shut down Low E 5 Pink Not connected a 6 Yellow Power on High DE 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 1200 Series Nano Pump Se
28. T T 36 4 Potential Cause Corrective Action Air in pump chamber of channel B or seals not yet seated Loose active inlet valve in channel B Loose pump head screws in channel B Loose outlet valve in channel B Leaking seal or scratched plunger in channel B1 Defective active inlet valve in channel B Flush channel B thoroughly with isopropanol under pressure restriction capillary Tighten the active inlet valve in channel B 14mm wrench Do not overtighten Ensure the pump head screws in channel B are tight Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Exchange the pump seals in channel B Check the plungers for scratches Exchange if scratched Exchange the active inlet valve in channel B 1200 Series Nano Pump Service Manual 95 2 96 Troubleshooting and Test Functions EMPV Test Step 1 Step 2 Step 3 Description The test is designed to verify the performance of the EMPV The test must always be done when the EMPV valve is exchanged The test should also be done if column flow stability problems occurs micro mode only The EMPV test is not a substitute for the leak test or pressure test The leak and pressure tests should also be done when leaks within the pump heads might be the problem The test starts with a short flushing sequence and a cleaning procedure for the EMPV Afterwards low and high pressure is controlled by the E
29. Table 8 EMPV failed to initialize Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes The no flow pressure of the system Set the flow to zero and disconnect the blue This problem typically causes is higher than 10 bar flexible capillary going from the damper to the a permanent EMPV mixer The system pressure reading should be Initialization not ready close to zero bar message e Ifthe system pressure reading is higher than 4 bar call Agilent service or refer to the Nano Pump Service Manual The inlet to the EMPV has been e Make sure the solvent channels are well purged This problem typically causes blocked or partially restricted The Check the EMPV filter Perform a pump purge at an EMPV Initialization Failed EMPV cannot take in sufficient 1000 pl min using pure water During the purge error message flow to deliver the correct flow check system pressure If pressure is gt 10 bar output The EMPV initialization replace the filter in front of the EMPV routine cannot be done within the Check the flow path from the damper outlet to the required 2 minute period EMPV inlet for plugs or restrictions Check the EMPV to flow sensor capillary for a total plug or partial restriction Replace the capillary or backflush the capillary with acetone Replace the EMPV assembly G1361 60000 Call Agilent service or refer to the Nano Pump Service Manual Exchanging the EMPV Assembly on page
30. The leak sensor in the nano pump has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes e Defective leak sensor e Leak sensor incorrectly routed being pinched by a metal component Suggested Actions V Exchange the leak sensor 1200 Series Nano Pump Service Manual 45 2 Troubleshooting and Test Functions Compensation Sensor Open The ambient compensation sensor NTC on the CSM board in the nano pump has failed open circuit The resistance across the temperature compensation sensor NTC on the CSM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable Causes e Defective CSM board Suggested Actions V Exchange the CSM board 46 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Compensation Sensor Short The ambient compensation sensor NTC on the CSM board in the nano pump has failed short circuit The resistance across the temperature compensation sensor NTC on the CSM board is dependent on ambient temperature The change in resistance
31. V Exchange the active inlet valve V Exchange the pump drive assembly V Exchange the CSM board 1200 Series Nano Pump Service Manual 75 2 76 Troubleshooting and Test Functions Wait Time out When running certain tests in the diagnostics mode or other special applications the pump must wait for the plungers to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the time out period otherwise the error message is generated Possible Reasons for a Wait Time out e Pressure not reached Pump channel A did not reach the delivery phase e Pump channel B did not reach the delivery phase e Pump channel A did not reach the take in phase e Pump channel B did not reach the take in phase e Solvent volume not delivered within the specified time Probable Causes e System still in purge mode Leakat fittings EMPV active inlet valve outlet ball valve or plunger seals e Flow changed after starting test e Defective pump drive assembly Suggested Actions V Ensure that purge valve is closed V Exchange defective capillaries V Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal active inlet valve outlet ball valve plunger seal V Ensure correct operating condition for the special application in use V Exchange the defective pump drive assembly 1200 Series Nano Pump Service
32. blank nut are tight Repeat the pressure test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened Ifthe test fails again insert the blank nut at the outlet of the previous module in the stack and repeat the pressure test Exclude each module one by one to determine which module is leaking e If the pump is determined to be the source of the leak run the EMPV cleaning procedure repeat the pressure test and if the test fails again run the leak test 1200 Series Nano Pump Service Manual 83 2 84 Troubleshooting and Test Functions Potential Causes of Pressure Test Failure After isolating and fixing the cause of the leak repeat the pressure test to confirm the system is pressure tight Potential Cause Pump Corrective Action Loose or leaky fitting Tighten the fitting or exchange the capillary Damaged pump seals or plungers Run the leak test to confirm the leak Potential Cause Autosampler Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Needle seat Exchange the needle seat Rotor seal injection valve Exchange the rotor seal Damaged metering seal or plunger Exchange the metering seal Check the plunger for scratches Exchange the plunger if required
33. detected by the processor the error message is generated Probable Causes e Disconnected or defective cable e Disconnected or defective connection cable front panel to CSPM board e Defective active inlet valve Suggested Actions V Ensure the pins of the active inlet valve connector are not damaged Ensure the connector is seated securely Y Ensure the connection cable is seated correctly Exchange the cable if defective V Exchange the active inlet valve 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Electro Magnetic Proportional Valve EMPV Missing EMPV Missing The EMPV in the micro pump is missing or defective Probable Causes e Disconnected or defective cable Defective solenoid Suggested Actions V Ensure the connection cable is seated correctly V Exchange the solenoid of the EMPV 1200 Series Nano Pump Service Manual 65 2 Troubleshooting and Test Functions Flow sensor missing Probable Causes e Flow sensor disconnected e Defective flow sensor Suggested Actions Y Ensure the sensor is seated correctly V Exchange the flow sensor 66 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Unsupported Flow Sensor Probable Causes e A 20 ul min or a 100 ul min flow sensor is used with the nano pump Suggested Actions V Change to the nano flow sensor 1200 Series Nano Pump Service Manual 67 2 Troubleshooting and Test Fu
34. enter a module serial number type the following command into the command line print sendmodule 1pmp ser YYYYYYYYYY Where YYYYYYYYYY is the 10 character serial number of the module in the question The first two characters are letters which should be capitalized The reply line will respond with RA 0000 SER followed by the module serial number you just entered 1200 Series Nano Pump Service Manual 149 3 Repairing the Pump To change the type of the module use the following command print sendmodule 1pmp TYPE XXXXX Where XXXXX is the 5 character product number of the module e g G22226A To configure the pump as G2226A nano pump requires the correct main board version If you enter the wrong type your module will not be accessible anymore In such a case see Changing the Product Number and Serial Number on page 149 or Using the Control Module G1323B on page 151 for recovering 150 Turn OFF the module then ON again Then restart the Agilent ChemStation If the serial number you have just entered is different than the original module serial number you will be given the opportunity to edit the configure 1200 access screen during the restart of the Agilent ChemStation After restart the serial number type you have just entered can be seen under the Instrument menu of the main interface screen Using the Instant Pilot G2408A Connect the Instant Pilot to the detector Turn ON the d
35. for HOX2 Filter 259 Agilent Technologies on Internet 260 Index 261 1200 Series Nano Pump Service Manual 12 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 1 Installing the Pump Site Requirements 14 Unpacking the Nano Pump 17 Optimizing the Stack Configuration 19 Installing the Nano Pump 24 Get the System Ready for the First Injection 29 ig Agilent Technologies 1 Installing the Pump Site Requirements A suitable environment is important to ensure optimum performance of the nano pump Power Consideration The nano pump power supply has wide ranging capability see Table 1 on page 15 It accepts any line voltage in the range described in the above mentioned table Consequently there is no voltage selector in the rear of the nano pump There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply To disconnect the nano pump from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off CAUTION Make sure to have easy access to the power cable of the instrument in order to disconnect the instrument from the line Power Cords Different power cords are offered as options with the nano pump The female end of each of the power cords is identical It plugs into the power input socket at the rear of the nano pump The male end of each of the powe
36. gold seal It should be exchanged when strongly deformed Place the valve in an upright position insert the sieve into the recess and replace the gold seal with the cap Make sure that the sieve cannot move and is away from the seal area of the gold seal 5 Place a new sieve into the recess of the outlet ball valve and replace the cap with the gold seal 6 Check that the new valve is assembled correctly and that the gold seal is present 124 1200 Series Nano Pump Service Manual Repairing the Pump 3 Valve body Sieve O 2 Gold seal Plastic cap Figure 16 Outlet Ball Valve Parts 7 Reinstall the outlet ball valve and tighten the valve 8 Reconnect the valve capillary Valve capillary Outlet ball valve Figure 17 Exchanging the Outlet Ball Valve 1200 Series Nano Pump Service Manual 125 3 Repairing the Pump Installing the Manual Purge Valve Frequency When performing a leak test for verifying pump tightness When using the nano pump permanently in normal mode Tools required Wrench 1 4 inch Wrench 14 mm Wrench 4 mm Parts required Purge valve assy G1311 60009 Adapter G1312 23200 Screw 0515 0175 Washer 2190 0586 Installing the Purge Valve ao ar O N 126 Switch off the pump module Disconnect the capillary from the mixer to the filter at the filter inlet Connect the capillary end into the purge valve holder Adapt the purge valve holder on the pump head of channel A Use th
37. indicates a leak rate greater than 3 ul min Plunger B1 delivers 50 ul min for approximately 7 seconds Plunger B1 delivers with a flow rate of 3 ul min for approximately 30 seconds The slope should be horizontal or slightly positive A negative slope indicates a leak rate greater than 3 ul min At the end of the fourth plateau the test is finished and the nano pump switches off Running the Leak Test Tools required Wrench 1 4 inch Parts and materials Restriction Capillary G1313 87305 required Blank nut 01080 83202 Isopropanol 500ml 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail This test requires the manual purge valve installed and the Electronic Flow Control EMPV ente sand flow sensor bypassed To install the manual purge valve see Installing the Manual Purge Valve on page 126 The slopes and plateaus are evaluated automatically Evaluating the Results of the Leak Test on page 89 describes the evaluation and interpretation of the leak test results Running the test from the ChemStation 1 Select the leak test from the test selection box in the Diagnosis screen 2 Start the test and follow the instructio
38. is offering a changing restriction to the pump The vacuum micro degasser is off or has become defective Basic performance problems in the pump Perform the EMPV cleaning procedure followed by the EMPV performance test Refer to the Nano Pump Service Manual EMPV Cleaning on page 103 Remove the blocked capillary clean it with Acetone Change the capillary Replace the analytical column Replace the filter frit in front upstream of the EMPV Try another vacuum micro degasser or experiment to determine the performance using different degasser channels If the mobile phase is very sensitive to gaseousness use the micro degasser continuous mode Perform the pump Leak Test Refer to the Nano Pump Service Manual Leak Test on page 85 1200 Series Nano Pump Service Manual 109 2 Troubleshooting and Test Functions Poor peak shape The peak shape has taken on a fronting or tailing characteristic Table 10 Poor peak shape Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes Column performance has deteriorated Poorly made capillary connections causing excessive dead volume or leaks ina chromatographically significant area of the system Capillaries which are internally broken especially those capillaries located in a chromatographically significant area Try a new column Using a flashlight and absorbent tissue carefully check for leaks thro
39. maintenance needs to be scheduled 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 The Electronics The electronics are comprised of four main components e The nano pump main board NPM see page 221 e The power supply see page 242 Optional e The interface board BCD external contacts see page 229 e The interface board LAN see page 230 Nano pump main board NPM The board controls all information and activities of all assemblies within the nano pump The operator enters parameters changes modes and controls the nano pump through interfaces CAN GPIB or RS 232C connected to the user interfaces Figure 40 and Figure 41 show block diagrams of this board ASIC Application Specific Integrated Circuit The application specific integrated circuit ASIC includes all digital logic for the core processor functions and also for module specific functions Motor Drive There are two identical motor drives for the drive assemblies Each drive comprises motorcontrol motor amplifier drive and current control Active Inlet Valve Drive There are two identical valve drives for the two active inlet valves Each drive comprises two amplifiers for the two contacts of valve solenoid Solvent Selection Valve Drive This drive consists of amplifiers for the solenoids of the solvent selection valve 1200 Series Nano Pump Service Manual 221 6 222 Introduction to the Nano Pump Electromagneti
40. module cover or overtightened Replace the column Otherwise backflush the column or replace the column inlet frit Perform a pump purge at 1000 pl min using pure water During the purge check system pressure If pressure is gt 10 bar replace the EMPV filter Using the sampler maintenance positions switch For severe capillary tube the sampler injection valve from mainpass to plugs acetone is a good bypass If pressure is significantly reduced backflushing solvent Backflush or change the needle seat assembly Replace the needle Backflush or replace the sample loop capillary Replace the injection valve rotor seal Clean the stator head with acetone and make sure the stator head ports are free of particles Refer to the system flow diagram One at a time disconnect the capillaries in the following order When the defective capillary is found it may be backflushed with acetone or replaced EMPV to flow sensor capillary flow sensor to sampler injection valve capillary sampler injection valve to column inlet capillary flow cell assembly includes inlet and outlet capillaries 106 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 EMPV failed to initialize micro mode only An attempt to pump in the micro mode has resulted in either an EMPV Initialization Failed error message or a permanent EMPV Initialization not ready message Make sure the system pressure is higher than 20 bar
41. of the counters Both liquimeters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 1200 Series Nano Pump Service Manual 219 6 220 Introduction to the Nano Pump Seal Wear Counters The seal wear counters display a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Using the EMF Counters The user settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of pump components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When performance indicates maintenance is necessary take note of the values displayed by liquimeters and seal wear counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that
42. panel is turned off 26 1200 Series Nano Pump Service Manual Install the Degasser G1379B Installing the Pump 1 CAUTION Ensure the line power switch at the front of the degasser is off 1 Place the degasser on top of the pump Make sure that the two modules are interlocked correctly 2 Connect one end of the remote cable 5061 3378 to the rear of the degasser Connect the other end of the cable to the remote port at the rear of the pump 3 The degasser accessory kit has a set of 4 solvent tubes G1322 67300 Each tube is labeled A B C or D Connect each solvent tube to its intended OUTLET channel port on the degasser Connect the other end of the solvent tube to its intended port at the pump solvent selection valve Follow the guide below Degasser Pump Solvent Selection OUTLET Valve Port A to Al left half upper B to A2 left half lower C to B1 right half upper D to B2 right half lower Install the Solvent Cabinet 1 The solvent cabinet accessory kit has 4 bottle head assemblies G1311 60003 2 Connect a bottle head assembly to each of the degasser INLET ports Use the labels provided with each bottle head assembly to appropriately label each bottle head assembly 3 Purge your system before first use see Get the System Ready for the First Injection on page 29 1200 Series Nano Pump Service Manual 27 1 Installing the Pump De i a 6 Figure 6
43. pump assembly B For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the connector P8 P21 at the main board Unclip the mixing chamber from its holder Disconnect the fan cable P17 at the main board and slide the fan towards the front and move it out of the unit 1200 Series Nano Pump Service Manual Repairing the Pump 3 8 Place the new fan into the recess Make sure that the air flow is directed as indicated arrow on the fan points in the same direction as on the foam Connect the cable to the main board P17 9 Re install pump assembly B Reconnect all capillaries and connectors P8 P21 10 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 11 Re install the mixing chamber to its holder 12 Re install the flow sensor Reconnect the capillaries and the cable 13 Re install the EMPV Reconnect the capillaries the waste tube and the cable 14 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 167 1200 Series Nano Pump Service Manual 157 3 Repairing the Pump Exchanging a Pump Drive Never start the pump when the pump head is removed This may damage the pump drive 158 The nano pump has two pump drive assemblies The A drive is located on the left side and the B drive on the right side Freque
44. section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1200 series modules Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization via APG remote e error handling e diagnostic functions and so on or module specific functions like e internal events such as motor control flow rates and so on e calculation of compensation values for variable strokes and pressures 1200 Series Nano Pump Service Manual 227 6 6 228 Introduction to the Nano Pump Firmware Updates Firmware updates can be done using your user interface e instant pilot G4208A with files from USB memory stick or e handheld control module G1323 with files from a PC card or e a PC Firmware Update Tool with files hard disk or CD ROM The file naming conventions are XXXXx_WW_ZZ dl b where xxxx is the product number e g G2226A for the G2226A nano pump ww is the revision number for exam
45. so on For identification and location of the connectors Figure 5 on page 25 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 232 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board GPIB Interface A chemstation cannot be connected directly to the well plate sampler by GPIB The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies Table 35 Default Addresses Autosampler 28 RID 29 Pump 22 FLD 23 VWD 24 Autosampler HP 1050 18 Agilent 8453A 25 Pump HP 1050 16 DAD MWD 26 VWD HP 1050 10 Column Compartment 27 DAD HP 1050 17 CAN Interface The CAN is an intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement 1200 Series Nano Pump Service Manual 233 6 234 Introduction to the Nano Pump Remote Interface The APG remote connector may be used in combination with other analytical instruments from Agilent Technologies if you wan
46. still connected To disconnect the nano pump from line unplug the power cord The power supply still uses some power even if the switch on the front panel is turned off When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION The electronics of the nano pump will not allow operation of the nano pump when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the nano pump Always operate the nano pump with the top foam and top covers in place IElectronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use an ESD protection when handling electronic boards and components 142 The procedures in this section describe how to exchange internal parts You must remove the nano pump from the stack in order to open the main cover 1200 Series Nano Pump Service Manual Repairing the Pump 3 Removing the Top Cover and Foam Tools required Screwdriver Pozidriv 1 Preparations forthis Switch off nano pump at the main power switch procedure Disconnect the solvent inlet tubes from the solvent selection valve or the adapter at the active inlet valve Beware
47. store more than one set of parameters using the same 8 bit configuration switch twice for example for both GPIB and RS 232C GPIB Default Addresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses Table 39 Default Addresses for Agilent Series 1200 Modules Module Address Binary Address Pump 22 00010110 FLD 23 00010111 VWD 24 00011000 Agilent 8453A 25 00011101 DAD MWD 26 00011010 Column compartment 27 00011011 Autosampler 28 00011100 Well plate sampler no address 000000000000 RID 29 00011101 where 0 means that the switch is down and 1 means that the switch is up 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Communication Settings for RS 232C Communication The communication protocol used in this instrument supports only hardware handshake CTS RTS Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory Table 40 Communication Settings for RS 232C Communication Mode Select f 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Parity Bits Use the following tables for selecting the setting which you want to use for RS 232C communication The numbe
48. the analysis where required 1200 Series Nano Pump Service Manual 39 2 40 Troubleshooting and Test Functions Shutdown An external instrument has generated a shut down signal on the remote line The nano pump continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable Causes e Leak detected in another module with a CAN connection to the system e Leak detected in an external instrument with a remote connection to the system e Shut down in an external instrument with a remote connection to the system e The degasser failed to generate sufficient vacuum for solvent degassing Suggested Actions V Fix the leak in the external instrument before restarting the nano pump V Check external instruments for a shut down condition V Check the vacuum degasser for an error condition Refer to the Reference Manual for the Agilent 1200 Series vacuum degasser 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Remote Time out A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions e g a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable Causes
49. the connector at the Z panel Make sure you are in normal mode 5 After an exchange of the valve cartridge it may take several mL of pumping with the solvent used in the current application before the flow stabilizes at A ripple as low as it used to be when the system was still working properly 1200 Series Nano Pump Service Manual Plug Repairing the Pump 3 A olvent inlet la m n tube Active inlet valve Figure 15 Exchanging the Active Inlet Valve 1200 Series Nano Pump Service Manual 123 3 Repairing the Pump Exchanging the Outlet Ball Valve Sieve or the Complete Valve Frequency Sieve whenever the pump seals will be exchanged Valve if internally leaking Tools required Wrench 1 4 inch Wrench 14 mm Parts required Outlet ball valve G1312 60008 Sieve pack of 10 5063 6505 Before exchanging the outlet ball valve you can try to clean it in a sonic bath Remove the gold seal and the sieve Place the valve in upright position onto the plastic cap in a small beaker with alcohol Place in a sonic bath for 5 to 10 minutes Insert a new sieve and replace the gold seal 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet ball valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body 3 Remove the plastic cap with the gold seal from the outlet ball valve 4 Using a pair of tweezers remove the sieve Check the
50. two pumping units is proportioned in an electomagnetical proportional valve EMPV The remaining column flow is measured in a mass flow sensitive flow sensor The measured flow is compared with the user entered column flow setpoint The flow sensor controls the EMPV current causing the EMPV to correctly proportion the column flow The primary flow in excess of the required column flow volume is derivated to the waste Solvent degassing is not done directly in the pump A 4 channel low volume micro vacuum degasser available as a separate module provides degassed solvents to the pump channel inputs Solvent degassing is required for best flow stability and detector stability especially at the low flow rates required to run nano LC applications 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Pump drive Power supply NPM boar Damper Outlet valve Pump head A Flow sensor Active inlet valve Pump head E Leak sensor Solvent selection valve Figure 36 Overview of the Nano Pump Hydraulic Path Overview The nano pump is based on the Agilent 1200 binary pump and performs all the functions necessary for a nano flow solvent delivery system Basically these functions are e Low Pressure Metering and High Pressure Delivery e Solvent Compressibility Compensation e Variable Stroke Volume e Column Flow Measurement and Control Low pressure solvent metering and high pressu
51. use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclo sure of Software is subject to Agilent Tech nologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a haz ard It call
52. 129 1200 Series Nano Pump Service Manual 107 2 Troubleshooting and Test Functions Unstable column flow and or system pressure In the micro mode the pump flow control system is active The flow control system continuously measures the actual value of column flow and maintains the requested column flow despite changes in system restriction If the flow control becomes defective actual column flow hence system pressure will fluctuate If the system offers a changing restriction to the pump actual column flow will fluctuate as the pump tries to maintain flow against the changing restriction Therefore in the micro mode unstable column flow and unstable system pressure usually appear together Table 9 Possible Causes Suggested Actions Unstable column flow and or system pressure Possible Causes and Suggested Actions Notes The flow setpoint is below the recommended minimum value The system pressure is insufficient for reliable flow control micro mode Leaks somewhere in the system One or more solvent channels are not correctly purged Ripple might also be too high Dirty solvent inlet filters Solvent intake is being restricted Ripple might also be too high Make sure that the column flow setpoint is above the recommended minimum setpoint Make sure that there is at least 20 bar pressure being developed after the pump Add an additional capillary after the pump if required Use a
53. 240 volts AC Wide ranging 10 line frequency of 50 60 Hz Output 1 24V 45A maximum total power consumption of 24 V 3 and 36 V must not exceed 107 W Output 2 36 V 2 5 A maximum Output 3 5V 3A Output 4 15V 03A Output 5 15V 0 3A 1200 Series Nano Pump Service Manual 243 6 Introduction to the Nano Pump 244 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 7 Specifications Performance Specification Agilent 1200 Series Nano Pump 246 Performance Specification Agilent 1200 Series Micro Degasser 247 ig Agilent Technologies 245 7 Specifications Performance Specifications 246 Table 47 Performance Specification Agilent 1200 Series Nano Pump Type Specification Hydraulic system Settable column flow range Recommended column flow range Optimum composition range Composition precision Delay volume Pressure range Compressibility compensation Recommended pH range Control and data evaluation Analog output Communications Two dual piston in series with proprietary servo controlled variable stroke drive floating piston active inlet valve solvent selection valve and electronic flow control for flow rates from 0 1 to 1 pl min 0 01 4 pl min 1 2500 pl min with the electronic flow control bypassed 0 1 1 pl min 200 2500 l min with the electronic flow sensor bypassed 1 to 99 or 5 pl min per channel primary f
54. 4 6 Agilent 1200 1 Not connected 2 Black Analog 3 Red Analog Vai x 194 1200 Series Nano Pump Service Manual Remote Cables Identifying Cables 5 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to 3390 Integrators Connector Pin Pin Signal Name Active 01046 60203 3390 Agilent 1200 TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low NC 4 Blue Shut down Low 2 D NC 5 Pink Not connected 6 NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low 1200 Series Nano Pump Service Manual 195 5 196 Identifying Cables Agilent 1200 to 3392 3 Integrators Connector Pin Pin Signal Name Active 01046 60206 3392 3 Agilent 1200 TTL 3 1 White Digital ground E x NC 2 Brown Prepare run Low f J 11 3 Gray Start Low O NC 4 Blue Shut down Low B 9 j NC 5 Pink Not connected ee ee NC 6 Yellow Power on High 9 7 Red Ready High 4 Key 1 8 Green Stop Low NC 9 Black Start request Low Agilent 1200 to 3394 Integrators Connector Pin Pin Signal Name Active 01046 60210 3394 Agilent 1200 TTL 9 1 White Digital ground
55. 9 on the main board see Figure 22 on page 147 6 Using a hex key unscrew the complete EMPV and remove it Screw a new one in place 8 Reconnect the capillaries going to the flow sensor coming from the filter Run the EMPV test see EMPV Test on page 96 1200 Series Nano Pump Service Manual 129 3 Repairing the Pump Exchanging the Solvent Selection Valve If internally leaking crossfoot between the ports or if one of the channels is Frequency blocked Tools required Screwdriver Pozidriv 1 Parts required Solvent selection valve PN gives half of a complete solvent selection block G1312 60000 1 Disconnect the solvent tubes and the active inlet valve connection tubes from the solvent selection valves Place solvent tubes into the solvent cabinet to prevent leaks due to hydrostatic flow ine in Solvent tubes Il ll gl i 5 TT Solvent J ks Saye a selection LD E a valve Connecting tubes Figure 20 Exchanging the sol
56. Agilent 1200 Series Nano Pump 7 Service Manual y fe Agilent Technologies Notices Agilent Technologies Inc 2006 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G2226 90110 Edition Edition 02 06 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Microsoft is a U S registered trademark of Microsoft Corporation Software Revision This guide is valid for A 01 xx revisions of the Agilent 1200 Series Nano Pump soft ware where xx refers to minor revisions of the software that do not affect the technical accuracy of this guide Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing
57. Critical Decision Determine if the problem is in the DAD or coming from the LC system Remove the flow cell from the DAD Close the cell cover and see if the baseline performance improves 1 If baseline performance has not improved a Replace the lamp s b Evaluate the environment for excessive drafts temperature changes etc 2 If baseline performance has improved focus attention on the possible causes and suggested actions below Table 12 Wandering Detector Baseline Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes Dirty or defective flow cell The analytical column Mixing Noise when pumping a binary mobile phase from two channels Unstable flow and or system pressure Clean or replace the flow cell Bypass the column Pump directly into the flow cell If performance improves try a new column Try premixing the mobile phase in one bottle and pumping 100 from that one solvent channel If baseline performance improves a solution must be found which is a compromise between mixing volume and other chromatographic requirements For an isocratic analysis premixing and pumping 100 one channel is the best solution Refer to the problem Unstable flow and or system pressure above If your system suffers from this problem go through the possible causes and suggested actions described there This problem occurs when one or both of the solvents has a high backgroun
58. Flow connection of the capillary pump Table3 Connecting capillaries for the Nano Pump Item Description Part Number 1 Stainless steel capillary G1311 67304 2 Stainless steel capillary G1312 67300 3 Stainless steel capillary G1312 67302 4 Stainless steel capillary G1312 67304 5 Stainless steel capillary 01090 87308 6 Stainless steel capillary G1375 87400 7 Peek coated fused silica capillary 220 mm 25 um G1375 87321 8 Peek coated fused silica capillary 390 mm 25 um G1375 87322 9 Peek coated fused silica capillary 550 mm 25 um G1375 87323 28 1200 Series Nano Pump Service Manual Installing the Pump 1 Get the System Ready for the First Injection When you are using the system for the first time after installation best results are obtained by performing the following 2 step system preparation in the order given below 1 Manually priming the solvent channels 2 Purging the pump WARNING When opening capillary or tube fittings solvents may leak Please observe appropriate safety precautions such as eye protection safety gloves protective clothing as described in the material handling information and safety data sheet supplied by the solvent vendor especially when hazardous solvents are used Manually Pirming the Solvent Channels This procedure should be done before the modules are turned on 1 The degasser accessory kit contains a 20ml plastic syrings and a solvent tube adapter for syringe Push the adapter on
59. IN Pink 6 Vent 202 1200 Series Nano Pump Service Manual Identifying Cables 5 CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1200 Series module s CAN bus connectors Agilent 1200 module to module 0 5 m 5181 1516 Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module G1323 81600 1200 Series Nano Pump Service Manual 203 5 Identifying Cables External Contact Cable One end of this cable provides a 15 pin plug to be connected to Agilent 1200 Series module s interface board The other end is for general purpose Agilent 1200 Series Interface Board to general purposes Connector Color Pin Signal Name G1103 61611 Agilent 1200 White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 el ui Red 8 EXT 4 dn _ Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 204 1200 Series Nano Pump Service Manual Identifying Cables 5 RS 232 Cable Kit This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Aligent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1200 module to PC RS 232 Cable Kit 34398As Instrument PC RX 2 2 RX TX 3 X 3 TX DTR 4
60. Leaky mixer if installed Loose pump head screws in channel A or B Leaking seal or scratched plunger in channel A2 or B2 Leaking outlet valve in channel A or B Leaky damper Ensure all fittings are tight or exchange capillary Tighten the mixer fittings and nuts Ensure the pump head screws in channels A and B are tight Exchange the pump seals in both channels Check the plungers for scratches Exchange if scratched Exchange the outlet valve Exchange damper 1200 Series Nano Pump Service Manual 91 2 Troubleshooting and Test Functions First plateau negative or unstable and at least one other plateau positive T T T T T T T T 0 as 1 15 2 25 3 35 4 Potential Cause Corrective Action Leaking outlet valve in channel A Clean the outlet valve in channel A Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Loose pump head screws in channel A Ensure the pump head screws in channel A are tight Leaking seal or scratched plunger in channel A2 Exchange the pump seals in channel A Check the plunger for scratches Exchange if scratched 92 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Second plateau negative or unstable and at least one other plateau positive T o 08 Potential Cause Corrective Action Leaking outlet valve in channel B Loose pump head screws in channel B Leaking sea
61. MPV and the appropriate current is monitored Finally a linear pressure ramp is performed Running the test 1 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Micro Mode Pressure Test 2 Start the test and follow the online instructions Nano Pump EMPY Test ojx Start Explain Close Result Status Nano PumpEMPv Test o d o Expected total time approx 15min i e rr rrrr Test Procedwe ooo o 1 Connect bottle with aqueous A1 and organic solvent 1 2 Release system pressure 3 Block EMP outlet with blank nut 01080 83202 4 Opening EMPY o o o 5 Start flushing both channels together about 25min Jo o o y B Execute EMP cleaning procedure about 2min _ 7 Closing EMP o 8 Running EMPY test about 8min __ 9 Opening EMP to release pressue 10 Evaluating EMP test results TT 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Evaluating the results The test results are evaluated automatically Two pressure plateaus are checked one at 20 bar and the other at 300 bar The appropriate current is measured A further criterion is the difference between the two plateaus The limits are as follows e lt 85 EMPV current for the 20 bar plateau e gt 15 EMPV current for the 300 bar plateau e gt 15 difference between both EMPV current values 1200 Series Nano Pump Service Manual 97 2 98
62. O NC 2 Brown Prepare run Low 80 3 3 Gray Start Low e e NC 4 Blue Shut down Low o a NC 5 Pink Not connected Che ie NC 6 Yellow Power on High O 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 1200 Series Nano Pump Service Manual Identifying Cables 5 START and STOP are connected via diodes to pin 3 of the 3394 connector Agilent 1200 to 3396A Integrators Connector Pin Pin Signal Name Active 03394 60600 3394 Agilent 1200 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low gt 35 3 3 Gray Start Low n i NC 4 Blue Shut down Low o NC 5 Pink Not connected i E NC 6 Yellow Power on High Eo 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1200 to 3396 Series II 3395A Integrators Use the cable 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1200 Series Nano Pump Service Manual 197 5 Identifying Cables Agilent 1200 to 3396 Series III 3395B Integrators Connector Pin Pin Signal Name Active 03396 61010 33XX Agilent 1200 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low i 15 3 3 Gray Start Low NC 4 Blue Shut down Low i o NC 5 Pink Not connected i a we NC 6 Yellow Power on High La 14 7 Red Ready High 4 8 Green
63. SST gold EMPV SST ruby sapphire PEEK Flow Sensor SST 1200 Series Nano Pump Service Manual 213 6 214 Introduction to the Nano Pump Table 30 Nano Pump Details continued Damping Unit Gold SST Capillaries Fused Silica For pump specifications see Specifications on page 245 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example aging of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value for each pump head independently The compensation value for each pump head can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume When a compressibility compensation value for a pump head is set the pump processor calculates a compensation volume that depends on the system pressure and the selected compressibility value This compensation volume is added to the delivery stroke of the first piston How Does Variable Stro
64. Test Procedure ul 11 Connect bottle with isoprop G22264 A 2 oand 2 Opening EMP __ 3 Stat flushing channel for about 2min __ 4 Closing EMY __ 5 Disconnect capillary at low sensor outlet __ 6 Put beaker under flow sensor outlet __ 7 Flushing flow sensor 200u Imin __ 8 Release system presse __ 9 Reconnect capilar at flow sensor outlet __ 10 Block system with blank mt __ 11 Execute EMPV cleaning procedure 12 ClsingEMPY __ E 13 Running pressure test about 45min __ 14 Opening EMPV to release pressue __ 15 Evaluating pressuretest PP Micro Mode Pressure Test Results The test results are evaluated automatically The sum of all leaks within the column flow path from the EMPV to the blank nut must be lower than100 nl min NOTE Small leaks with no visible leaks in the flow path can cause the test to fail 78 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 If the pressure test fails Ensure all fittings between the pump and the blank nut are tight and repeat the pressure test If the test fails again insert the blank nut at the outlet of the previous module in the stack and repeat the pressure test Exclude each module one by one to determine which module is leaky Potential Causes of Pressure Test Failure After isolating and fixing the cause of the leak repeat the pressure test to confirm the system is tight Potential Cause Pump Correc
65. This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter LAN cable Cross over network cable shielded 3 m long 5023 0203 for point to point connection Twisted pair network cable shielded 7 m long 5023 0202 for hub connections 1200 Series Nano Pump Service Manual 191 5 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent 1200 Series modules The other end depends on the instrument to which connection is being made Agilent 1200 to 3390 2 3 Integrators Connector Pin Pin Signal Name 01040 60101 3390 2 3 Agilent 1200 1 Shield Ground 2 Not connected 8 _ 3 Center Signal 7 ne H EG 4 Connected to pin 6 w SIRE 7 a lan 5 Shield Analog i BRNA 6 Connected to pin 4 7 Key 8 Not connected 192 1200 Series Nano Pump Service Manual Identifying Cables 5 Agilent 1200 to 3394 6 Integrators Connector Pin Pin Signal Name 35900 60750 3394 6 Agilent 1200 1 Not connected 2 Shield Analog 3 Center Analog GIN 559 Agilent 1200 to BNC Connector Connector Pin Pin Signal Name 8120 1840 BNC Agilent 1200 Shield Shield Analog Center Center Analog 1200 Series Nano Pump Service Manual 193 5 Identifying Cables Agilent 1200 to General Purpose Connector Pin Pin Signal Name 01046 60105 339
66. V Ensure the damper is connected correctly to the CSM board V Exchange the damper 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Valve Failed Valve 0 Failed valve Al Valve 1 Failed valve A2 Valve 2 Failed valve B2 Valve 3 Failed valve B1 One of the solvent selection valves in the nano pump failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable Causes e Solvent selection valve disconnected e Connection cable inside instrument not connected e Connection cable inside instrument defective e Solvent selection valve defective Suggested Actions V Ensure the solvent selection valve is connected correctly V Ensure the connection cable is connected correctly V Exchange the connection cable V Exchange the solvent selection valve 1200 Series Nano Pump Service Manual 55 2 Troubleshooting and Test Functions Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure readings from the damper every Ims If the readings are missing for longer than 10 seconds the error message is generated Probable Causes e Damper not connected e Defective damper Defective CSM board Suggested Actions V Ensure the damper connector is clean and seated correctly V Exchange
67. a 4 mm hexagonal key step wise loosen and remove the two pump head screws and remove the pump head from the pump drive Pumphead screws Place the pump head on a flat surface Loosen the lock screw two revolutions While holding the lower half of the assembly carefully pull the pump head away from the plunger housing a Pump head Lock screw 3 Remove the support rings from the plunger housing and lift the housing away from the plungers Support rings Plunger housing Plunger 1200 Series Nano Pump Service Manual 133 3 Repairing the Pump Exchanging the Pump Seals and Seal Wear in Procedure When required Seals leaking if indicated by the results of the leak test Check both pump heads individually Tools required 3 mm hexagonal key 4 mm hexagonal key 1 4 inch wrench Parts required Seals pack of 2 5063 6589 standard or 0985 1420 for normal phase application For the seal wear in procedure Restricton capillary 5022 2159 1 Disassemble the pump head assembly of the leaky pump head see Removing and Disassembling the Pump Head Assembly on page 132 134 1200 Series Nano Pump Service Manual Repairing the Pump 3 2 Usingone of the plungers carefully remove the seal from the pump head be careful not to break the plunger Remove wear retainers if still present Plunger Seal 3 Clean the pump chambers wi
68. and connectors P16 P11 P20 10 Re install the mixing chamber to its holder 11 Re install the pump head Reconnect the capillaries and the cable 12 Re install the flow sensor Reconnect the capillaries and the cable 13 Re install the EMPV Reconnect the capillaries the waste tube and the cable 14 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 167 1200 Series Nano Pump Service Manual 159 3 160 Repairing the Pump Exchanging the Power Supply Frequency If defective Tools required Screwdriver Pozidriv 1 e Wrench 1 4 inch Wrench 14 mm Wrench7mm Wrench5mm Parts required Power supply 0950 2528 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 143 2 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 3 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page
69. anging the Outlet Ball Valve Sieve or the Complete Valve 124 Installing the Manual Purge Valve 126 Installing the Purge Valve 126 Exchanging the Purge Valve Frit or the Complete Manual Purge Valve 127 Exchanging the EMPV Assembly 129 Exchanging the Solvent Selection Valve 130 Removing and Disassembling the Pump Head Assembly 132 Exchanging the Pump Seals and Seal Wear in Procedure 134 Exchanging the Plungers 137 Exchanging the Flow Sensor 138 Reassembling the Pump Head Assembly 139 Exchanging the Optional Interface Board 141 Exchanging Internal Parts 142 Removing the Top Cover and Foam 143 Exchanging the Nano Pump Main Board NPM Board 146 Changing the Product Number and Serial Number 149 Using the Agilent ChemStation 149 Using the Instant Pilot G2408A 150 Using the Control Module G1323B 151 Replacing the Nano Pump s Firmware 153 Exchanging the Damper 154 Exchanging the Fan 156 1200 Series Nano Pump Service Manual Exchanging a Pump Drive 158 Exchanging the Power Supply 160 Exchanging the Leak Sensor 162 Exchanging Status Light Pipe 165 Assembling the Main Cover 166 Replacing the Top Cover and Foam 167 4 Parts and Materials Nano Pump Main Assemblies 172 Solvent Cabinet and Bottle Head Assembly 174 Nano Pump Hydraulic Path 176 Pump Head Assembly 178 Electro Magnetic Proportional Valve EMPV 180 Flow Sensor Assembly 181 Power and Status Light Pipes 182 Leak Parts 183 Cover Parts 184 Sheet Metal Kit 185 F
70. are This connector needs to be activated by the configuration switch module next to the GPIB connector The software needs the appropriate drivers to support this communication See your software documentation for further information The power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA Volt Amps There is no voltage selector on your nano pump because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents that the nano pump cover is taken off when line power is still connected The interface board slot is used for external contacts BCD output LAN and for future use Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 216 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Configuration switch Slot for interface board S SS q C Remote 7 i o TEESE Analog output 99 i CAN Bus ui O jo A V100 1207220 240 pE pi AE kia
71. auxiliary 202 cables BCD 200 cables CAN 203 cables external contact 204 cables LAN cables 206 parts main assemblies 172 performance specification 246 pH range 246 physical specification 15 piston 120 137 power consideration 14 Power consumption 16 power consumption 16 power light pipe 182 power supply 160 description 242 specifications 243 power supply indicator 35 power input socket 14 prerun condition 36 pressure above upper limit 52 pressure below lower limit 53 Pressure Converter 222 pressure plot leak test 89 pressure pulsation 214 pressure sensor readings 26 pressure test 34 80 pressure operating range 246 PTFE frit 120 126 127 pump head assembly 178 pump head missing 70 pump housing parts 172 pump seals 120 134 purge valve 120 126 127 purging pump 29 30 reassembling the pump head 139 recommended pH range 246 remote connector 216 removing the foam 143 removing the pump head 132 removing the top cover 143 repair procedures 120 replacing the foam 167 replacing the top cover 167 restart without cover 50 results pressure test 83 RS 232C cable kit to PC 205 communication settings 239 interface 235 settings 239 RS 232C connector 216 run mode 36 running the leak test 86 running the pressure test 81 S safety standards 16 safety light switch 167 screwdriver pozidriv 1 130 138 154 156 158 160 162 165 166 167 seal wear count
72. ball valve e High friction partial mechanical blockage in the drive assembly e Defective drive assembly e Defective CSPM board Suggested Actions V Ensure the capillaries and frits between the pump head and damper inlet are free from blockage V Exchange the outlet ball valve V Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or drive assembly V Exchange the drive assembly V Exchange the CSM board 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Encoder Missing Encoder Missing Pump channel A B Encoder Missing Pump channel B The optical encoder on the pump motor in the nano pump is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable Causes e Defective or disconnected pump encoder connector e Defective pump drive assembly Suggested Actions V Ensure the connector is clean and seated correctly V Exchange the pump drive assembly 1200 Series Nano Pump Service Manual 63 2 64 Troubleshooting and Test Functions Inlet Valve Missing Inlet Valve Missing Pump channel A B Inlet Valve Missing Pump channel B The active inlet valve in the nano pump is missing or defective The processor checks the presence of the active inlet valve connector every 2 seconds If the connector is not
73. bleshooting and Test Functions Status Indicators 35 Error Messages 37 Micro Mode Pressure Test 77 Basic System Troubleshooting 104 This chapter describes the pump s built in troubleshooting and test functions Status Indicators The nano pump is provided with two status indicators which indicate the operational state prerun run and error states of the nano pump The status indicators provide a quick visual check of the operation of the nano pump Status Indicators on page 35 Error Messages In the event of an electronic mechanical or hydraulic failure the nano pump generates an error message in the user interface The following pages describe the meaning of the error messages For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided Error Messages on page 37 Micro Pressure Test This is a fast test to verify the tightness of a micro system where the pump is operating in the micro mode and no manual purge valve is installed The pump is operating in the pressure control mode at 380 bar for several minutes The remaining flow in the column flow path between the EMPV and the blank nut is measured Micro Mode Pressure Test on page 77 ee Agilent Technologies 33 2 34 Troubleshooting and Test Functions Pressure Test The pressure test is a quick built in test designed to demonstrate the press
74. cal Proportional Valve The EMPV consists of an electro magnetic solenoid driven by a PWM Pulse Width Modulator Pressure Converter This block comprises a filter and amplifier for the pressure sensor signal a multiplexer an A D converter and an offset correction for the analog pressure output signal The output voltage is 2 mV bar Leak Converter This block comprises a PTC for the leak identification and a NTC for the ambient temperature measurement This assures that temperature changes are not identified as leak A leak would cool down the PTC and its change in resistance results in a leak signal Fan Drive The revolution of the fan is controlled by the main processor depending on the internal heat distribution in the nano pump The fan provides a PWM signal which is proportional to the revolution This fan status signal is used for diagnostics Electronic Fuses The valve circuits are electronically fused on the board Any error on the board or shortages of the valves will activate the electronic fuses that will switch off the supply voltage This prevents the damage of components Onboard Battery An onboard lithium battery buffers the electronic memories when the nano pump is turned off For safety information on lithium batteries see General Safety Information on page 250 1200 Series Nano Pump Service Manual Introduction to the Nano Pump Interfaces The nano pump provides the following interfaces e tw
75. ccantar control i Drive Fan Senso Flow Control signal Data address Diagnose signals digital Diagnose signals analog Temperature of hybrid gt Block Diagram Pump Controller Board 225 6 Introduction to the Nano Pump 24V Electroni AVB Z panel Inlet valve eg Sctronie drive B fuse ia 24V motor B Poo j Motor encoder B drive B 15V E Hardware 36V switch la 24V motor A 7 Motor drive A sufi Processor encoder A de 15V i AVA Z panel Inlet valve 36V Electronic Hardware ja P drive A 24V fuse lt switch 24V SSV valve lt 36V Electronic 4 4 j SSV lt Z panel ka drive B ak I fuso SSV valve 4 36V Electronic 4 drive A M 424V fuse EMPV a Leak Leak 15V converter g sensor 24V Fan drive a Fan 15V Damper converter jgk ins gt Flow 36V lt lt sensor CSM 24V lt power Power 15V supply supply 5V lt Figure 42 Interconnection Diagram NPM Board 226 1200 Series Nano Pump Service Manual Introduction to the Nano Pump Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific
76. ch 237 control module parts 190 cover parts 182 D damper 154 damping unit 154 degreaser spray 134 delay volume 19 delivery checklist 17 description leak test 85 dimensions 15 disassembling the pump head 132 E early maintenance feedback EMF 218 electrical connections 216 electronic fuses 216 222 electronics HPM board 221 electrostatic discharge ESD 117 EMF counter 219 EMF flag 219 EMF limits 220 EMPV cleaning procedure 103 EMPV parts 180 EMPV test 96 encoder missing 63 environment 14 15 error zero solvent counter 51 error condition 36 1200 Series Nano Pump Service Manual error message wait timeout 76 error messages 33 36 compensation sensor open 46 compensation sensor short 47 encoder missing 63 fan failed 48 ignition without cover 49 index adjustment 72 index limit 71 index missing 73 initialization failed 75 inlet valve fuse 59 inlet valve missing 64 eak 43 eak sensor open 44 eak sensor short 45 missing pressure reading 56 motor drive power 62 pressure above upper limit 52 pressure below lower limit 53 pump head missing 70 restart without cover 50 selection valve failed 54 selection valve fuse 58 servo restart failed 65 shut down 40 stroke length 74 synchronization lost 41 temperature limit exceeded 61 temperature out of range 60 timeout 39 ESD Strap 118 exchanging active inlet valve 120 121 damper
77. continued Pin Direction Function 8 In CTS 9 In RI strument 0 QONDUIUAWN COI FP HPWH DB9 DB9 DB9 Male Female Female Figure 45 RS 232 Cable 236 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Setting the 8 bit Configuration Switch The 8 bit configuration switch is located next to the GPIB connector Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures Figure 46 8 bit Configuration Switch Table 38 8 bit Configuration Switch Mode Select 2 3 4 5 6 7 8 GPIB 0 0 GPIB Address RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC Switches 1 and 2 define which set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory 1200 Series Nano Pump Service Manual 237 6 238 Introduction to the Nano Pump In the non volatile memory the parameters are kept regardless of whether you turn the instrument off and on again They will be kept until the same set of parameters is subsequently changed and power is reset All other previously stored configuration settings will still remain in the non volatile memory In this way you can
78. d absorbance at the detection wavelength In this case the pump s mixing efficiency may not be good enough to produce a sufficiently homogeneous mobile phase The detector reacts to gluts of the more detectable parts of the solvent mixture and baseline disturbances result Failure to maintain stable column flow or system pressure can also cause unwanted baseline activity 112 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 User interface displays error messages for specific modules Table 13 User interface displays error messages for specific modules Possible causes and suggested actions Possible Causes Suggested Actions Notes A module has experienced a Refer to the Reference Manual supplied with A specific error message for that specific hardware failure the module Follow the advice on module is displayed during operation troubleshooting and repair for the error message The status indicator of that module displayed is red 1200 Series Nano Pump Service Manual 113 2 Troubleshooting and Test Functions 114 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 3 Repairing the Pump Introduction 117 Simple Replacements 117 Exchanging Internal Parts 117 Cleaning the Nano Pump 118 Using the ESD Strap 118 Overview about the Nano Pump 119 Simple Repair Procedures 120 Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 121 E
79. der 20 Re install the flow sensor Reconnect the capillaries and the cable 21 Re install the EMPV Reconnect the capillaries the waste tube and the cable 22 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 167 1200 Series Nano Pump Service Manual 163 3 Repairing the Pump To main board J23 Figure 24 Exchanging the Leak Sensor 164 1200 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging Status Light Pipe Frequency If part is broken Tools required Screwdriver Pozidriv 1 Parts required Status light pipe 5041 8384 Preparation for this procedure Remove the front cover and top cover see Removing the Top Cover and Foam on page 143 1 The status light pipe is clipped into the top 2 Replace the top cover see Replacing the cover Top Cover and Foam on page 167 3 Replace the nano pump into the stack and reconnect the cables and capillaries 4 Turnon the nano pump 1200 Series Nano Pump Service Manual 165 3 Repairing the Pump Assembling the Main Cover Frequency If cover is broken Tools required None Parts required Cover kit G1312 68713 includes base top left and right The cover kit contains all parts but it is not assembled WARNING In case you insert the left or right side in the opposite position you may not be able to remove the side from the t
80. e Defective pump drive assembly Suggested Actions V Ensure the encoder cable are not damaged or dirty Make sure the cables are connected securely to the CSM board V Exchange the pump drive assembly 1200 Series Nano Pump Service Manual 73 2 74 Troubleshooting and Test Functions Stroke Length Stroke Length Pump channel A B Stroke Length Pump channel B The distance between the lower plunger position and the upper mechanical stop is out of limits nano pump During initialization the nano pump monitors the drive current If the plunger reaches the upper mechanical stop position before expected the motor current increases as the nano pump attempts to drive the plunger beyond the mechanical stop This current increase causes the error message to be generated Probable Causes Defective pump drive assembly Suggested Actions V Exchange the pump drive assembly 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Initialization Failed Initialization Failed Pump channel A B Initialization Failed Pump channel B The nano pump failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable Causes e Blocked active inlet valve e Defective pump drive assembly e Defective CSM board Suggested Actions
81. e screw and the washer to fix the purge valve holder Insert the purge valve assembly into the purge valve holder and locate the outlet and waste outlet as shown below Use the 14 mm wrench to tighten the purge valve assembly Connect the waste tube from the EMPV assembly to the waste outlet of the purge valve Connect the pump outlet capillary into the outlet of the purge valve 1200 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the Purge Valve Frit or the Complete Manual Purge Valve Frequency Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 2500 pl min of H70 with purge valve opened Purge valve if internally leaking Tools required Wrench 1 4 inch Wrench 4 mm depending on pump outlet capillary Wrench 14 mm Pair of tweezers or toothpick Parts required PTFE Frit pack of 5 01018 22707 Purge valve G1311 60009 Purge valve holder Pump outlet capillary Waste capillary Figure 18 Exchanging the Purge Valve Frit Exchanging the purge valve frit or the complete purge valve 1 Using a 1 4 inch or 4 mm wrench disconnect the pump outlet capillary at the purge valve 1200 Series Nano Pump Service Manual 127 3 128 Repairing the Pump 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it from the purge val
82. e total main flow is diverted to waste 210 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Degasser From solvent bottle From solvent bottle Pump head B amp Piston Inlet valve EMPV Flow sensor Damper Filter To waste Figure 37 The Hydraulic Path How Does the Pumping unit Work Both pumping units channel A and channel B are identical with respect to parts and function Each pumping unit consists of a pump head which is directly attached to a metering drive assembly In each metering drive assembly a servo controlled variable reluctance motor and gear train assembly are used to move two ball screw drives The gear train moves the two ball screw drives in opposite directions 180 degree out of phase The gear ratios are designed such that the first ball screw drive constantly moves at twice the speed of the second ball screw drive The servo motor includes a high resolution shaft position encoder which continuously 1200 Series Nano Pump Service Manual 211 6 212 Introduction to the Nano Pump reports the speed and direction of the motor in real time This speed and direction information is used by the pump control electronics to ensure precise control of the servo motor movement Each pump head consists of two identical chambers pistons and seals plus an active inlet valve and an outlet ball valve The solvent volume in each chamber is d
83. eat the test Ifvalue still out of range accept the offset only at the end of the test If the flow sensor has a negative offset the minimum flow rate the pump can operate is the numeric value of the measured offset For example If offset flow is 0 030 pL min the minimum flow rate the pump can operate is 30 nL min The offset of the nano flow sensor has a more significant influence on the flow sensor accuracy than the linearity 102 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 EMPV Cleaning Description Depending on the application sometimes particles can be collected in the EMPV valve This fast cleaning routine is designed to remove such particle deposits The routine should always be performed when the EMPV is suspected of being leaky or contaminated with particles Step1 The outlet of the EMPV is plugged with a blank nut After a short flushing routine the EMPV is closed and the pressure is increased to approximately 380 bar Step 2 The EMPV is then opened and the pressure is released very quickly Step3 This procedure is repeated several times in a sequence Running the Cleaning procedure 1 Select the ChemStation Diagnosis screen from the maintenance selection box select EMPV Cleaning 2 Start the test and follow the online instructions EMPY Cleaning Procedure Ioj x Start Explain Close Result Status Nano Pump EMPY Cleaning Procedure IR Expected
84. ed Actions Notes Leaks somewhere in the Use a flashlight and absorbent tissue to search At very low flow rates a leak may system for leaks throughout the system never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see Solvent channels are not Perform a 4 minute purge at 2500 pl min for This is especially likely if the system correctly purged Ripple each solvent channel has been unused for more than one might also be too high day Dirty solvent inlet filters Clean or replace the solvent inlet filters To minimize this problem prefilter Solvent intake is being the mobile phase and take restricted Ripple might precautions against algae formation also be too high in the water 1200 Series Nano Pump Service Manual 105 2 Troubleshooting and Test Functions System Pressure Abnormally High The current system pressure is significantly above the typical system pressure produced by this method with this column Table 7 System Pressure Abnormally High Possible causes and actions Possible Causes Suggested Actions Notes The analytical column has become plugged The filter in front upstream of the EMPV has become plugged A component in the micro sampler has become plugged This could be the sample loop needle needle seat assembly or injection valve ports A capillary before or after the sampler in the system has become plugged broken crushed by a
85. el A Inlet Valve Fuse 1 Pump channel B One of the active inlet valves in the nano pump has drawn excessive current causing the inlet valve electronic fuse to open Probable Causes e Defective active inlet valve e Defective connection cable front panel to CSM board e Defective CSM board Suggested Actions Y Restart the nano pump If the error message appears again exchange the active inlet valve V Exchange the connection cable V Exchange the CSM board 1200 Series Nano Pump Service Manual 59 2 60 Troubleshooting and Test Functions Temperature Out of Range Temperature Out of Range 0 Pump channel A Temperature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable Causes Defective CSM board Suggested Actions V Exchange the CSM board 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Temperature Limit Exceeded Temperature Limit Exceeded 0 Pump channel A Temperature Limit Exceeded 1 Pump channel B The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the CSM board If excessive current is being drawn for long periods the temperature of the circuits increase
86. ensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the CSM board Probable Causes e Loose fittings e Broken capillary e Loose or leaking active inlet valve outlet ball valve or EMPV e Defective pump seals Suggested Actions V Ensure all fittings are tight V Exchange defective capillaries V Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal active inlet valve outlet ball valve V Exchange the pump seals 1200 Series Nano Pump Service Manual 43 2 44 Troubleshooting and Test Functions Leak Sensor Open The leak sensor in the nano pump has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable Causes e Leak sensor not connected to the CSM board Defective leak sensor e Leak sensor incorrectly routed being pinched by a metal component Suggested Actions V Ensure the leak sensor is connected correctly V Exchange the leak sensor 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Leak Sensor Short
87. er 135 220 seals 120 security lever 117 selection valve failed 54 selection valve fuse 58 servo restart failed 65 setable flow range 246 sheet metal kit 185 shipping container 17 shut down 40 sieve 120 124 simple repair procedures 120 site requirements 14 solvent selection valve 130 solvent selection valve drive 221 sonic bath 124 specification performance 246 physical 15 stack configuration 19 stack configuration front view 20 stack configuration rear view 21 status light pipe 165 182 1200 Series Nano Pump Service Manual Index stroke length 74 stroke volume 214 synchronization lost 41 T temperature limit exceeded 61 temperature out of range 60 timeout 39 top cover 143 167 tweezers 127 U unpacking the pump 17 uv radiation 256 V variable stroke volume 214 voltage range 15 216 voltage selector 216 WwW wait timeout 76 wall socket 14 wash seals 120 138 weight 15 wideranging capability 14 wrench 1 4 inch 81 86 124 126 127 129 132 154 156 158 160 162 wrench 14mm 121 124 126 127 129 146 162 wrench 5mm 146 162 wrench 7mm 146 162 Z zero solvent counter 51 263 Index 264 1200 Series Nano Pump Service Manual www agilent com In This Book This manual contains technical reference information about the Agilent 1200 Series Nano pump The manual describes the following installation e optimizing perfor
88. et valve missing 64 installation pump module 24 instrument status indicator 36 interface board 141 interface board BCD LAN 229 interfaces 223 analog signal output 233 APG remote 234 CAN 233 GPIB 233 overview 232 RS 232C 235 internet 260 introduction to the pump 208 L laboratory 15 laboratory bench 15 lamp instrument status 36 lamp power supply 35 LAN cables 206 interface board 229 LAN interface board 230 eak 43 eak converter 222 eak sensor 162 eak sensor open 44 eak sensor short 45 eak test 34 85 eak test evaluation 89 ight pipes 182 ine frequency 16 216 ine voltage 15 216 iquimeter 135 219 ogbook 39 M main assemblies parts 172 ain assemblies overview 119 main cover 166 maintenance procedures 219 message ignition without cover 49 issing pressure reading 56 otor drive 221 otor drive power 62 Si 33 3 non operating altitude 16 non operating temperature 16 not ready condition 36 0 onboard battery 222 operating altitude 16 operating temperature 16 optimum performance 19 outlet ball valve 120 124 overview pump 209 P parts control module 190 cover 182 EMPV parts 180 flow sensor 181 1200 Series Nano Pump Service Manual foam 186 hydraulic path 176 light pipes 182 pump head 178 pump housing 172 sheet metal kit 185 parts identification cables analog 192 cables APG remote 195 cables
89. etector On the Instant Pilot s Welcome Screen press More then select Maintenance Using the up down arrows select the autosampler where you have to change the product number or serial number Press PN SN This will display a screen where you can enter the product number and or serial number Make your changes using the information from the product label of your module To configure the pump as G2226A nano pump requires the correct main board version 1200 Series Nano Pump Service Manual Repairing the Pump 3 WARNING If you enter the wrong type your module will not be accessible anymore In sucha case see Changing the Product Number and Serial Number on page 149 or Using the Control Module G1323B on page 151 for recovering 5 Press OK to highlight the complete command 6 Press Done to transfer the information into the main board s memory Press Cancel to quit the process 7 Turn the detector OFF then ON again The Maintenance screen should display the correct serial number for this module 8 Ifan Agilent ChemStation is also connected restart the Agilent ChemStation now as well Recover Instructions Turn OFF the detector 2 Change the 8 bit Configuration Switch to Resident see Stay Resident Settings on page 241 3 Turn the detector ON 4 Re do steps 2 to 5 of Changing the Product Number and Serial Number on page 149 and correct the type information Enter the product number
90. ett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 These cards may be longer orderable Minimum firmware of these Hewlett Packard JetDirect cards is A 05 05 Recommended Cables Cross over network cable shielded 3m long 5023 0203 for point to point connection Twisted pair network cable shielded 7m long 5023 0202 for hub connections 1200 Series Nano Pump Service Manual 231 6 Introduction to the Nano Pump Interfaces The Agilent 1200 Series modules provide the following interfaces Table 34 Agilent 1200 Series Interfaces Interface Type Pumps Autosampler DA Detector DA Detector VW Detector TCC Degasser MW Detector MW Detector RI Detector FL Detector G1315C G1365C CAN Yes LAN on board No GPIB Yes RS 2323C Yes Remote Yes Analog Yes LAN BCD Ext Yes Yes Yes Yes Yes Yes No No No Yes No No No Yes Yes No Yes No No Yes Yes Yes Yes Yes No Yes Yes Yes Yes Yes Yes No 2x 2x 1x No Yes Yes Yes No Yes No No The vacuum degasser will have a special connector for specific use For details see description of main board e CAN connectors as interface to other Agilent 1200 Series modules e GPIB connector as interface to the Agilent ChemStation e RS 232C as interface to a computer REMOTE connector as interface to other Agilent products e Analog Output connector s for signal output and e Interface slot for specific interfacing external contacts BCD LAN and
91. ettings 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Stay Resident Settings Firmware update procedures may require this mode in case of firmware loading errors Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication Table 45 Stay Resident Settings Mode Select 1 2 3 4 5 6 7 8 TEST BOOT 1 1 0 0 1 0 0 0 To return to normal operation set switches back to your GPIB or RS 232C configuration settings 1200 Series Nano Pump Service Manual 241 6 Introduction to the Nano Pump The Main Power Supply Assembly 242 The main power supply comprises a closed assembly no onsite repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting MPS 12V supply gt 36V 2 5A floating primary 24V circuits gt Line v
92. fied type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided 1200 Series Nano Pump Service Manual CAUTION Appendix 8 The operator of this instrument is advised that ifthe equipment is used in a manner not specified in this manual the protection provided by the equipment may be impaired Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are prese
93. flashlight and absorbent tissue to search for leaks throughout the system Check for leaks after the pump and inside the pump valves fittings etc If operating in the micro mode perform the micro mode pressure test If operating in the normal mode perform the normal mode pressure test Perform a 2 minute purge at 2500 pl min for each solvent channel Temporarily remove solvent inlet filters to see if they are the cause of the problem If so clean or replace the solvent inlet filters Normal mode 100 pl min Micro mode 20 pl flow sensor 1 pl min Micro mode 100 ul flow sensor 10 l min At very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see Refer to the Nano Pump Service Manual Micro Mode Pressure Test on page 77 Normal Mode Pressure Test on page 80 This is especially likely if the system has been unused for more than one day To minimize this problem prefilter the mobile phase and take precautions against algae formation in the water 108 1200 Series Nano Pump Service Manual Table 9 Troubleshooting and Test Functions 2 Unstable column flow and or system pressure Possible Causes and Suggested Actions continued Possible Causes Suggested Actions Notes Dirty EMPV micro mode only One of a capillary after the EMIPV is partially or completely blocked Any system component which
94. for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the nano pump from the stack removing the covers and disassembling the nano pump The security lever at the power input socket prevents that the pump cover is taken off when line power is still connected To prevent personal injury the power cable must be removed from the nano pump before opening the cover Do not connect the power cable to the nano pump while the covers are removed CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD protection for example the ESD wrist strap from the accessory kit when handling electronic boards and components 1200 Series Nano Pump Service Manual 117 3 Repairing the Pump Cleaning the Nano Pump The nano pump case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth as liquid can drop into the nano pump Do not let liquid drip into the nano pump It could cause shock hazard and it could damage the nano pump Using the ESD Strap CAUTION Elec
95. fter the damper Defective damper e Defective CSM board Suggested Actions V Ensure the upper pressure limit is set to a value suitable for the analysis V Check for blockage in the flowpath V Exchange the damper V Exchange the CSM board 52 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable Causes e Lower pressure limit set too high e Air bubbles in the mobile phase Leak e Defective damper e Defective CSM board Suggested Actions V Ensure the lower pressure limit is set to a value suitable for the analysis V Ensure solvents are degassed Purge the nano pump Y Ensure solvent inlet filters are not blocked V Inspect the pump head capillaries and fittings for signs of a leak V Purge the nano pump Run a pressure test to determine whether the seals or other pump components are defective v Exchange the damper V Exchange the CSM board 1200 Series Nano Pump Service Manual 53 2 54 Troubleshooting and Test Functions Pressure Signal Missing The pressure signal from the damper is missing The pressure signal from the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable Causes Damper disconnected e Defective damper Suggested Actions
96. g the Pump 1 9 l AC power Analog signal to i SK TE A recorder G7 Remote cable EE E ao jo se AC power CAN Bus cable AC power AC power Analog signal to recorder GPIB or LAN AC power to LC ChemStation Figure 2 Recommended Stack Configuration Rear View 1200 Series Nano Pump Service Manual 21 1 Installing the Pump TOTT fe Detector ace Instant Pilot oo olumn Compartment DI I E s Solvent Cabinet Autosampler SSD gt J Degasser ALS Thermostat Pump D Ci Figure 3 Recommended stack configuration front view 22 1200 Series Nano Pump Service Manual Installing the Pump 1 GPIB or LAN to LC ChemStation
97. he corresponding flow sensor response is measured The pump is delivering solvent for 2 minutes at a pressure equal to the initial pressure divided by 10 At the end of this step the corresponding flow sensor response is measured The pump is delivering solvent for 2 minutes at a pressure equal to the initial pressure divided by 20 At the end of this step the corresponding flow sensor response is measured The EMPV is opening to release the pressure No primary flow is delivered After 2 minutes the flow sensor offset and pressure offset are measured 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Make absolutely sure that all parts of the flow path that are included in the test are very thoroughly flushed with WATER before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path will definitely cause the test to fail It is absolutely necessary that the pump is tight We recommend to run the Micro Mode Pressure Test to verify the tightness of the pump Running the Test 1 Prepare the restriction capillary G2226 67300 r 5 D quartz capillary SST fitting front and back PEEK sleeve ferrule Slide the PEEK sleeve over the fused silica capillary Slide the SST fitting over the PEEK sleeve Slide the front and back ferrule over the PEEK sleeve SC A O N Carefully press the capillary into an adjust
98. ications Type Specification Comments Weight 17 kg 39 Ibs Dimensions 180 x 345 x 435 mm height x weight x depth 7 x 13 5 x 17 inches Line voltage 100 120 or 220 240 VAC 10 Wide ranging capability 1200 Series Nano Pump Service Manual 15 1 Installing the Pump Table1 Physical Specifications continued Line frequency Power consumption apparent power Power consumption active power 50 or 60 Hz 5 220 VA Maximum 75 W Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL 4 55 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Non condensing Up to 2000 m 6500 ft Up to 4600 m 14950 ft For storing the nano pump Installation Category Il Pollution Degree 2 16 1200 Series Nano Pump Service Manual Installing the Pump 1 Unpacking the Nano Pump CAUTION Damaged Packaging Upon receipt of your nano pump inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the nano pump has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carrier s inspection If there are signs of damage to the nan
99. ies to ensure proper functionality and compliance with safety or EMC regulations 190 Table 28 Cables Overview Type Description Part Number Analog 3390 2 3 integrators 01040 60101 cables 3394 6 integrators 35900 60750 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote 3390 integrator 01046 60203 cables 3392 3 integrators 3394 integrator 3396A Series I integrator 3396 Series Il 3395A integrator see page 197 3396 Series Ill 3395B integrator Agilent 1200 1050 modules 1046A FLD 1046A FLD 35900A A D converter 1090 liquid chromatographs Signal distribution module 01046 60206 01046 60210 03394 60600 03396 61010 5061 3378 5061 3378 5061 3378 01046 60202 01046 60202 1200 Series Nano Pump Service Manual Table 28 Cables Overview continued Identifying Cables 5 Type Description Part Number BCD 3396 integrator 03396 60560 cables General purpose spade Lugs G1351 81600 Auxiliary Agilent 1200 Series vacuum degasser G1322 61600 CAN Agilent 1200 module to module 0 5 m 5181 1516 cables Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module G1323 81600 External Agilent 1200 Series interface board to general purpose G1103 61611 contacts GPIB Agilent 1200 module to Agilent ChemStation 1 m 10833A cable Agilent 1200 module to Agilent ChemStation 2 m 10833B RS 232 Agilent 1200 module to a computer 34398A cable
100. is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable Causes e Defective CSM board Suggested Actions V Exchange the CSM board 1200 Series Nano Pump Service Manual 47 2 Troubleshooting and Test Functions Fan Failed The cooling fan in the nano pump has failed The hall sensor on the fan shaf t is used by the CSM board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated Probable Causes e Fan cable disconnected e Defective fan e Defective CSM board e Improperly positioned cables or wires obstructing fan blades Suggested Actions V Ensure the fan is connected correctly V Exchange fan V Exchange the CSM board Y Ensure the fan is not mechanically blocked 48 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Open Cover The top foam has been removed The sensor on the CSM board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable Causes e The top foam was removed during operation e Foam not activating the sensor e Sensor defective e Rear of the module is exposed to strong direct sunlight Suggested Actions V Replace the top foam V Exchange the CSM board V Ensure that the rear of the
101. isplaced by its piston The pistons are directly moved by the reciprocating ball screw drives of the metering drive assembly Due to the gear design of the metering drive assembly the pistons move in opposite directions with piston 1 constantly moving at twice the speed of piston 2 The outer diameter of the piston is smaller than the inner diameter of the chamber allowing solvent to flow in the gap between the piston and the chamber wall The two chambers are connected by the pressure dependent outlet ball valve The position of the solvent selection valve determines which of two solvents will be sucked low pressure through the active inlet valve into chamber 1 during the intake stroke of piston 1 The active inlet valve is electrically opened and closed making its operation more precise at low pressures The stroke volume of piston 1 is between 2 ul and 100 ul depending on flow rate When the nano pump is first turned on the user is prompted to initialize the pump The initialization routine occurring for both pump heads first determines the precise movement limits for both pistons These limits are then stored in the pump controller memory Then both pistons are set to their default initial positions When pumping begins the active inlet valve is opened and piston 1 begins its intake stroke sucking solvent into chamber 1 At the same time piston 2 begins its delivery stroke pumping high pressure the existing solvent in chamber 2 ou
102. ke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes will generate less pressure pulsations than higher stroke volumes at same flow rates In addition the frequency of the pressure pulsations will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes resulting in less flow ripple will improve composition ripple 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 The nano pump uses a processor controlled ball screw system to drive its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume The stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended When the pump is in the standard mode the EMPV is fully closed Total main flow up to 2500 ul min is directed to the LC system Column flow measurement control is disabled This mode is for non capillary LC applications In the micro mode the flow sensor measures and controls colu
103. keting agilent com start start asp To download and install always the newest available version of firmware on your system or call your local service provider for assistance 1200 Series Nano Pump Service Manual 153 3 154 Repairing the Pump Exchanging the Damper Frequency No pressure output or when leaking Tools required Screwdriver Pozidriv 1 Wrench 1 4 inch Parts required 79835 60005 Damper Do not totally remove the Z panel when the damper is in its place The heavy damper may fall down 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 143 2 Disconnect both active inlet valve cables 3 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 4 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 5 Unclip the mixing chamber from its holder Remove the damper and the Z panel For this disconnect first the restriction capillary the capillary going to the mixer the connector P16 P11 P20 at the main board see Figure 22 on page 147 7 Unclip the mixing chamber from its holder 8 Loosen the screws of the Z panel fold it forward and remove the damper 9 Re install the Z panel and the new damper Reconnect the capillaries and connectors P16 P11
104. l Prevent any crystallization of buffer solutions This will lead into a blockage damage of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 75 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls 09 gt 2C0C1 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 1200 Series Nano Pump Service Manual 257 8 258 Appendix Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filte
105. l inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 218 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 Early Maintenance Feedback EMF Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The nano pump provides a series of EMF counters for the left and right pump heads Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The nano pump provides the following EMF counters e liquimeter pump A e seal wear pump A e liquimeter pump B and e seal wear pump B Liquimeters The liquimeters display the total volume of solvent pumped by the left and right pump heads since the last reset
106. l or scratched plunger in channel B2 Clean the outlet valve in channel B Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Ensure the pump head screws in channel B are tight Exchange the pump seals in channel B Check the plunger for scratches Exchange if scratched 1200 Series Nano Pump Service Manual 2 94 Troubleshooting and Test Functions Third plateau negative or unstable and at least one other plateau positive lie n Potential Cause Corrective Action Air in channel A or new seals not yet seated Loose active inlet valve in channel A Loose pump head screws in channel A Loose outlet valve in channel A Leaking seal or scratched plunger in channel A1 Defective active inlet valve in channel A Flush channel A thoroughly with isopropanol under pressure use restriction capillary Tighten the active inlet valve in channel A 14mm wrench Do not overtighten Ensure the pump head screws in channel A are tight Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Exchange the pump seals in channel A Check the plungers for scratches Exchange if scratched Exchange the active inlet valve in channel A 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Fourth plateau negative or unstable and at least one other plateau positive T T T T T T a 08 1 15 2 25 3
107. le carrying area of the system The 40 pl chamber of the micro sampler metering head has developed bubbles Using a flashlight and absorbent tissue carefully check for leaks in the following areas a All micro sampler valve ports b The junction of the needle and sample loop capillary c The needle seat interface d Column inlet and outlet e Flow cell inlet capillary at the capillary cell body junction Inthe user interface diagnostics access the Change Piston function of the micro sampler maintenance positions This function draws the metering piston fully inward clearing the chamber Under flow conditions activate this function for at least 5 minutes The sampler valve must be in the mainpass position at this time Any bubbles which have formed in the chamber will now be cleared by the flow At very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see In most applications only a small part of the available 40pl metering head volume is used At very low flows bubbles may form in the unused space between the metering piston and the chamber wall The bubbles act to defeat the draw of sample into the needle For best results in clearing bubbles the mobile phase being pumped should not contain water 1200 Series Nano Pump Service Manual 111 2 Troubleshooting and Test Functions Wandering Detector Baseline
108. low whatever is greater lt 0 2 SD at 500 nl min default settings Minimum primary flow pump channel is 5 pl min Typically 300 nl from the electronic flow control to the pump outlet for flow rates up to 4 pl min For flow rates up to 4 l min and electronic flow control active primary flow path 180 480 pl system pressure dependent default settings calculated volume Typically 180 to 480 ul system pressure dependent for flow rates up to 2 5 ml min default settings calculated volume 20 to 400 bar 5880 psi system pressure User selectable based on mobile phase compressibility 1 0 8 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Upper pH range is limited by fused silica capillaries Agilent ChemStation for LC For pressure monitoring 2 mV bar one output Controller area network CAN GPIB RS 232C APG Remote ready start stop and shut down signals LAN optional 1200 Series Nano Pump Service Manual Specifications 7 Table 47 Performance Specification Agilent 1200 Series Nano Pump continued Safety and maintenance Extensive diagnostics error detection and display through control module and Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pum
109. mance diagnostics and troubleshooting e repairing e parts and materials e introduction to the pump theory of operation e screens of the local control module and e specifications Agilent Technologies 2006 Printed in Germany Edition 02 06 90110 G2226 phe Agilent Technologies
110. ment union and tight the fitting moderately with the 4 mm wrench 8710 1534 and the torque adapter G1315 45003 to fix the ferrule To reach right values for the flow sensor accuracy calibration the restriction capillary G2226 67300 must not be damaged or blocked 6 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Flow Sensor Accuracy Calibration Test 7 Start the test and follow the online instructions 1200 Series Nano Pump Service Manual 99 2 Troubleshooting and Test Functions Nano Pump Flow Sensor Accuracy Calibration OF x Start Explain Close Result Status Nano Pump Flow Sensor Accuracy Calibration Test Procedure J o o O Expected total time approx 35min TC O o LI i Test Procedure Cd CY 1 The Flow Sensor must be warmed up for atleast 1 hour Sooo o y y 2 Connect the restriction capillary at the outlet of the flow sense oOo oo o y 3 Fil water in channel A1 Low amounts of salts are allowed O 4 Opening EMPY ooo o o 5 Start flushing channel A1 for5 min o e Closing EMPY ooo o o 7 Flushing restriction capilar with water oOo TC 8 Starting test ooo oo o __ __ __ as Be im __ __ i _ Lil Lu __ 8a Initial offset measurement about 2 min b Measuring pressure drop over pump inline filter about 1 min 8 The test results are evaluated automatically Test results In the ChemStation the mea
111. mn flow in the range of 0 1 ul min to 4 ul min Flow measurement is based on the principle of mass flow temperature sensitivity The flow sensor consists of a heated tube with two temperature sensors As the mobile phase passes through the heated tube the temperature characteristic distributed over the two temperature sensors is evaluated From the temperature characteristic flow rate accuracy is determined The flow sensor measurement is calibrated for specific mobile phases which are user selectable 1200 Series Nano Pump Service Manual 215 6 Introduction to the Nano Pump Electrical Connections The GPIB connector is used to connect the nano pump with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your nano pump The switches are preset to a default address see Table 35 on page 233 and is recognized once after power on The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1200 Series module data transfer and synchronization One analog output provides a signal for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on The RS 232 connector may be used to control the nano pump from a computer via RS 232 connection using appropriate softw
112. module is not exposed to strong sunlight 1200 Series Nano Pump Service Manual 49 2 50 Troubleshooting and Test Functions Restart Without Cover The nano pump was restarted with the top cover and foam open The sensor on the CSM board detects when the top foam is in place If the nano pump is restarted with the foam removed the nano pump switches off within 30 s and the error message is generated Probable Causes e The nano pump started with the top cover and foam removed Rear of the module is exposed to strong direct sunlight Suggested Actions v Replace the top cover and foam V Ensure that the rear of the module is not exposed to strong sunlight 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Zero Solvent Counter Pump firmware version A 02 32 and higher allow to set solvent bottle fillings at the ChemStation revision 5 xx and higher If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable Causes e Volume in bottle below specified volume Incorrect setting of limit Suggested Actions v Refill bottles and reset solvent counters 1200 Series Nano Pump Service Manual 51 2 Troubleshooting and Test Functions Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit Probable Causes e Upper pressure limit set too low e Blockage in the flowpath a
113. n This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1200 Series Nano Pump Service Manual 255 8 Appendix UV Radiation This information is only valid for UV lamps without cover e g 2140 0590 and 2140 0813 Emissions of ultraviolet radiation 200 315 nm from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 50 UV Radiation Limits Exposure day Effective Irradiance 8 hours 0 1 uW em 10 minutes 5 0 uW em Typically the radiation values are much smaller than these limits Table 51 UV Radiation Typical Values Position Effective Irradiance Lamp installed 50 cm distance Average 0 016 uW em Lamp installed 50 cm distance Maximum 0 14 W cm 256 1200 Series Nano Pump Service Manual Appendix 8 Solvent Information Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cel
114. nctions Leak Sensor Missing Probable Causes e Disconnected or defective cable e Defective leak sensor Suggested Actions V Ensure the connection cable is seated correctly V Exchange the leak sensor 68 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Servo Restart Failed Servo Restart Failed Pump channel A B Servo Restart Failed Pump channel B The pump motor in the nano pump was unable to move into the correct position for restarting When the nano pump is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable Causes e Disconnected or defective cables e Mechanical blockage of the nano pump e Defective pump drive assembly e Defective CSM board Suggested Actions V Ensure the pump assembly cables are not damaged or dirty Make sure the cables are connected securely to the CSM board V Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly V Exchange the pump drive assembly V Exchange the CSM board 1200 Series Nano Pump Service Manual 69 2 70 Troubleshooting and Test Functions Pump Head Missing
115. ncy Error message Motor Drive Power Tools required Screwdriver Pozidriv 1 Wrench 1 4 inch 4 mm hexagonal key Parts required Pump drive G1311 60001 exchange part number G1311 69001 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 143 2 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 3 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 4 Remove the pump head For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the active inlet valve cable at the Z panel 5 Unclip the mixing chamber from its holder 1200 Series Nano Pump Service Manual Repairing the Pump 3 6 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 147 7 Remove the pump drive For this disconnect first the connector P8 P21 or P12 P14 at the main board 8 Place the new pump drive into the recess Reconnect the connectors at the main board 9 Re install the Z panel and the damper Reconnect the capillaries
116. ns Lenk Test M anual Purge Yalye n of xi Start Explain Close Expected total time approx 15 min Test Procedure oo d S 1 Instal and connect manual purge valve __ 6 Connect restriction cap 61313 87305 to valve outlet a aa 9 e T_T E nes 7 Start fushing both channels with pressure about 3min __ a Open and close purge valve to release system pressure __ a Replace restiction cap with blank rut 07080 82202 f 10 Open purge valve for pump mitato CT Sd ti intime TT S 12 Close purge vee TC TC__ 13 Running leak test method abou 45min MST 14 Evahtingleaktestresuts TT T_ l 15 Open purge valve to release pressure 1200 Series Nano Pump Service Manual 87 2 Troubleshooting and Test Functions Running the test from the Control Module 1 Place two bottles of LC grade isopropyl alcohol in channels A2 and B2 2 Set flow to 2500 ul min for channel A2 and B2 and flush the degasser for about 2 minutes If the pumps seals were replaced or the seals are not sufficient settled use the following procedure e Connect the restriction capillary G1313 87305 to flow sensor outlet e Set flow to 2500 ul min normal mode and 50 B2 Pump for about 10 min e Stop the flow 3 Set flow to 0 ul min and replace the restriction capillary with blank nut 01080 83202 4 Connect the signal cable to the analog output at the rear of the nano pump only if an integrator is used 5 Press
117. nt in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1200 Series Nano Pump Service Manual 251 8 Appendix Safety Symbols Table 49 shows safety symbols used on the instrument and in the manuals Table 49 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage 4 Indicates dangerous voltages Indicates a protected ground terminal a Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in I this product WARNING A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions 252 1200 Series Nano Pump Service Man
118. o CAN connectors as interface to other Agilent 1200 Series modules e one GPIB connector as interface to the Agilent ChemStation e one RS 232C as interface to a computer e one REMOTE connector as interface to other Agilent products e one analog output for pressure signal output and e one optional interface board e LAN 1200 Series Nano Pump Service Manual 223 6 6 224 Introduction to the Nano Pump EMPV Drive Motor drive B Encoder B Pump DriveB head B Motor B Iv J 4 Control Current san Data control B address Active ING Valve drive B valve B SSV A SSVB 4 valve drives 5 ASIC 2 Co e a Active inlet Valve drive A valve A aa Option i 1 Current control A Motor Data address 4 control A Control Pump Motor A head A Drive A J Encoder A Motor drive A 2 Figure 40 Block Diagram Pump Controller Board 1200 Series Nano Pump Service Manual Multiplexer Figure 41 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 2 Filter amp P D A D converter amplifier lt Pressure amper Offset Pressure converter correction gt Analog output gt gt Integrator Amplifier amp ambient measurement Bd temperature lanl
119. o pump please do not attempt to install the nano pump Delivery Checklist Ensure all parts and materials have been delivered with the nano pump For this compare the shipment content with the checklist included in the instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Accessory Kit Each shipment contents an accessory kit with the necessary tools to install the pump and to have an operating system Table 2 Nano Pump Accessory Kit Content G2226 68705 Description Part Number Insert tool 01018 23702 SST Solvent inlet filter x4 01018 60025 Waste tube 2 m 0890 1760 SST replacement frit 0 5 um 5022 2185 Wrench open end 7 16 1 2 inch x 2 8710 0806 1200 Series Nano Pump Service Manual 17 1 Installing the Pump Table2 Nano Pump Accessory Kit Content G2226 68705 continued Description Part Number Wrench open end 1 4 5 16 inch x1 8710 0510 Wrench open end 14 mm x 1 8710 1924 Wrench open end 4 mm x 1 8710 1534 Hex key 2 5 mm 15 cm long straight handle x 1 8710 2412 Hex key 3 0 mm 12 cm long x 1 8710 2411 Hex key 4 0 mm 15 cm long T handle x 1 8710 2392 Torque adapter G1315 45003 CAN cable 1 m long 5181 1519 Purge valve assembly G1311 60009 Purge valve holder G1312 23200 Screw for the purge valve holder 0515 0175 PFS capillary 350 mm 25 pm G1375 87322 PFS capillary 550 mm 25 pm G1375 87323 PFS capillary 150 mm 75 pm
120. oam Parts 186 Nano Pump Accessory Kit G2226 68705 187 5 Identifying Cables Cable Overview 190 Analog Cables 192 Remote Cables 195 BCD Cables 200 Auxiliary Cable 202 1200 Series Nano Pump Service Manual 10 CAN Cable 203 External Contact Cable 204 RS 232 Cable Kit 205 LAN Cables 206 Introduction to the Nano Pump Introduction to the Nano Pump 208 Hydraulic Path Overview 209 How Does the Pumping unit Work 211 Electrical Connections 216 Instrument Layout 218 Early Maintenance Feedback EMF 219 EMF Counters 219 Using the EMF Counters 220 The Electronics 221 Nano pump main board NPM 221 Firmware Description 227 Resident System 227 Main System 227 Firmware Updates 228 Optional Interface Boards 229 BCD Board 229 LAN Communication Interface Board 230 Interfaces 232 Analog Signal Output 233 GPIB Interface 233 CAN Interface 233 Remote Interface 234 RS 232C 235 1200 Series Nano Pump Service Manual Setting the 8 bit Configuration Switch 237 GPIB Default Addresses 238 Communication Settings for RS 232C Communication Forced Cold Start Settings 240 Stay Resident Settings 241 The Main Power Supply Assembly 242 7 Specifications Performance Specifications 246 8 Appendix General Safety Information 250 239 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 253 Radio Interference 254 Sound Emission 255 UV Radiation 256 Solvent Information 257 Declaration of Conformity
121. of leaking solvents due to hydrostatic flow Remove leak funnel with the waste tube Remove the solvent cabinet from the nano pump 1 Remove the front cover by pressing the 2 Unplug the power cord and move the lever clip fastener on both sides of the cover towards the power socket 1200 Series Nano Pump Service Manual 143 3 Repairing the Pump 3 Lift the clips on both sides of the top cover 1 Remove the top cover 2 Unscrew the screws on the top plate and remove the plate by lifting its back first 5 Ifan optional interface board is installed remove it from the nano pump 144 1200 Series Nano Pump Service Manual Repairing the Pump 3 Do not connect a power plug to the nano pump module after removing the top covers A safety light switch on the main board will prevent operation when the covers are removed The next figure shows the position of the light switch on the board 7 Position of the safety switch on the main board Top foam Light switch Foam lever 1200 Series Nano Pump Service Manual 145 3 146 Repairing the Pump Exchanging the Nano Pump Main Board NPM Board Frequency Tools required Parts required Board defective Wrench 14 mm Wrench 7 mm Wrench 5 mm NPM board G2226 66530 exchange part number G2226 69530 1 Turn off the pump disconnect all cables and remove the pump from the stack
122. of the leak pan Waste funnel el i Waste tube holder 9 Place the nano pump back to its position in the stack replace solvent cabinet and reconnect all tubes and capillaries figure shows configuration with solvent selection valve 10 Replace the front covers and reconnect the power cable to the nano pump 11 Switch on the nano pump 1200 Series Nano Pump Service Manual 169 3 Repairing the Pump 170 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 4 Parts and Materials Nano Pump Main Assemblies 172 Solvent Cabinet and Bottle Head Assembly 174 Nano Pump Hydraulic Path 176 Pump Head Assembly 178 Electro Magnetic Proportional Valve EMPV 180 Flow Sensor Assembly 181 Power and Status Light Pipes 182 Leak Parts 183 Cover Parts 184 Sheet Metal Kit 185 Foam Parts 186 Nano Pump Accessory Kit G2226 68705 187 ee Agilent Technologies 171 Parts and Materials 4 Nano Pump Main Assemblies Nano pump main assembly Figure 25 1200 Series Nano Pump Service Manual 172 Parts and Materials 4 Table 16 Nano pump main assembly Item Description Part Number 1 Nano pump main board NPM G2226 66530 Exchange NPM board G2226 69530 2 Power supply 0950 2528 3 Solvent selection valve connecting cable G1312 61602 4 Flow sensor G1376 68704 5
123. ols required Wrench 14 mm Parts required Active inlet valve without cartridge G1312 60025 Valve Cartridge 5062 8562 Removing the Active Inlet Valve Unplug the active inlet valve cable from the connector 2 Disconnect the solvent inlet tube at the inlet valve Beware of leaking solvents 3 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head Valve body Ru Valve cartridge Figure 14 Active Inlet Valve Parts 1200 Series Nano Pump Service Manual 121 3 122 Repairing the Pump Exchanging the Valve Cartridge 1 Using a pair of tweezers remove the valve cartridge from the actuator assembly Before inserting the new valve cartridge clean the area in the actuator assembly Fill a syringe with alcohol and flush the cartridge area thoroughly Insert a new valve cartridge into the actuator assembly Make sure the valve cartridge is fully inserted into the actuator assembly Replacing the Active Inlet Valve 1 Insert the new valve into the pump head Using the 14 mm wrench turn the nut until hand tight Position the valve that the solvent inlet tube connection points towards the front Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Do not overtighten the valve The solvent inlet tube connection should point to the right corner of the pump head Reconnect the inlet tube and the active inlet valve cable to
124. oltage 24 V 4 5 A 5V switching p 5 V 3 A regulator sense linear 15V 3A voltage regulators pm 15 V 3A total power consumption on 36 V and 24 V must not exceed 107 watts Figure 47 Main Power Supply MPS Block diagram To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines When overload conditions occur the power supply turns off all output voltages Turning the line power off and on again resets the power supply to normal operation if the cause of the overload condition has been removed An over temperature sensor in the main power supply is used to turn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 46 Main Power Supply Specifications Maximum power 300 VA 200 W Continuous output Line Input 100
125. op part 1 Place the top part on the bench and insert 2 Replace the cover the left and right side into the top part 3 Replace the nano pump into the stack and reconnect the cables and capillaries 4 Turnon the nano pump 166 1200 Series Nano Pump Service Manual Repairing the Pump 3 Replacing the Top Cover and Foam Tools required Screwdriver Pozidriv 1 Preparations forthis Make sure that after your repair all assemblies cables capillaries and procedure connectors are located in its correct place 1 Place the damper cable in a curve to the 2 Make sure that the foam is installed right side towards the active inlet valve correctly and is located in the safety light cable of pump B Replace the top foam switch section BEE 1200 Series Nano Pump Service Manual 167 3 Repairing the Pump 3 Location of foam in the light switch Light switch 4 Replace the optional interface board or the board cover plate 5 Replace the metal cover slide the metal tabs into place 1 underneath the Z Panel in the front then lower the back of the metal plate 2 and fix the two holding screws 6 Replace the top cover 168 1200 Series Nano Pump Service Manual Repairing the Pump 3 7 Ensure clips are seated correctly move lever back 8 Replace the leak funnel with the waste tube Locate the lower end of the waste tube in the holder
126. p plungers Al and B1 are at the top of their stroke 1200 Series Nano Pump Service Manual 85 2 86 Troubleshooting and Test Functions Ramp 2 Ramp 3 Plateau 1 Ramp 4 Plateau 2 Ramp 5 Plateau 3 Ramp 6 Plateau 4 The nano pump continues pumping solvent with a flow rate of 150 ul min Channel A delivers for one pump cycle first plunger A2 delivers then plunger A1 followed by channel B plunger B2 then plunger B1 both channels with a stroke of 20 ul The pressure increase during this phase should be linear Large leaks or defects will be characterized by an unstable non linear slope Just before the start of the first plateau plunger A2 delivers with a flow rate of 50 ul min for approximately 8 seconds The system pressure should be 240 bar or higher At plateau 1 plunger A2 delivers with a flow rate of 3 ul min for 30 seconds During this time the slope should be horizontal or slightly positive slight pressure increase A negative slope indicates a leak rate greater than 3 ul min Plunger B2 delivers 50 ul min for approximately 8 seconds Plunger B2 delivers with a flow rate of 3 ul min for 30 seconds The slope should be horizontal or slightly positive A negative slope indicates a leak rate greater than 3 ul min Plunger A1 delivers 50 ul min for approximately 8 seconds Plunger A1 with a flow rate of 3 ul min for 30 seconds The slope should be horizontal or slightly positive A negative slope
127. panol 500 ml This test requires the manual purge valve installed and the Electronic Flow Control EMPV and flow sensor bypassed To install the manual purge valve see Installing the Manual Purge Valve on page 126 Make absolutely sure that all parts of the flow path that are part of the test are thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail Running the test from the ChemStation 1 Select the pressure test from the test selection box in the Diagnosis screen 2 Start the test and follow the instructions 1200 Series Nano Pump Service Manual 81 2 82 Troubleshooting and Test Functions Normal Mode Pressure Test OF x Start Explain Close Result Status Nano Pump Pressure Test Manual Purge Valve iL Expected total time approx 10min lt CT y ee rr 1 i Test Procedure ooo o o Running pressure test about 4 5 min Evaluating pressure test 9 Open purge valve to release pressure 6 7 8 Close purge valve LI The slope and plateau is evaluated automatically Evaluating the Results on page 83 describes the evaluation and interpretation of the pressure test results Running the test from the Control Module 1 Place a bottle of LC grade isopropyl alcohol in the solvent cabinet and connect it to channel A2 Block the injection device port 6 wi
128. ped mobile phase with user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclable Table 48 Performance Specification Agilent 1200 Series Micro Degasser Type Specification Maximum flow rate 5 ml min per channel Number of channels 4 Internal volume Typically 1 ml per channel pH range 1 14 RS232 output For diagnosis purpose Material in contact with PTFE FEP PEEK solvents The Agilent 1200 Series micro vacuum degasser has been tested for evaporation of solvents into the atmosphere by an independent institute with approved methods The tests were performed with Methanol BIA Nr 7810 and Acetonitrile NIOSH Nr 1606 Evaporation of these solvents into the atmosphere when operating the degasser was below the limits of detection 1200 Series Nano Pump Service Manual 247 7 Specifications 248 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 8 Appendix General Safety Information 250 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 253 Radio Interference 254 Sound Emission 255 UV Radiation 256 Solvent Information 257 Declaration of Conformity for HOX2 Filter 259 Agilent Technologies on Internet 260 This chapter provides addition information on safety legal and web ig Agilent Technologies 249 8 Appendix General Safety Information 250 The following general safet
129. ple A602 is the revision A 06 02 and zz is the build number of the firmware For instructions refer to the documentation provided with the Firmware Upgrade Tools provided on the Agilent web Update of main system can be done in the resident system only Update of the resident system can be done in the main system only main FW update Resident System Main System resident FW update Figure 43 Firmware Update Mechanism 1200 Series Nano Pump Service Manual Optional Interface Boards Introduction to the Nano Pump 6 The Agilent 1200 Series modules have one optional board slot that allows addition of an interface board to the modules Table 31 Optional Interface Boards Description Part Number BCD Board Fuse 250 mA four are on the board LAN Communication Interface Board G1351 68701 2110 0004 G1369A or G1369 60001 BCD Board The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused Board identificatio Processo BCD r register External contacts E E ce 12 gt Line driver 250 mA cis 4x Figure 44 Block Diagram BCD Board 1200 Series Nano Pump Service Manual BCD connector
130. pump assemblies For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the connector P8 P21 and P12 P14 at the main board 162 1200 Series Nano Pump Service Manual Repairing the Pump 3 7 Disconnect the fan cable P17 at the main board 8 Remove the solvent selection valve See exchanging the solvent selection valve in this chapter 9 Remove the CSPM board Disconnect the remaining connectors see exchanging the high pressure pump main board in this chapter 10 Push the leak sensor cable through the recess of the solvent selection valve and lift out the bottom foam 11 Unclip the leak pan and place it in front of the nano pump 12 Pull the leak sensor out of the leak pan and lift the cable out of the metal plate 13 Place the new leak sensor into the leak pan see Figure 24 and replace the pan Make sure that the cable is located in the recess of the bottom metal plate 14 Reinstall the bottom foam Make sure that the leak senor cable is guided through the foam to the top side 15 Replace the main board see Exchanging the Nano Pump Main Board NPM Board on page 146 16 Re install the solvent selection valve 17 Re install the two pump head Reconnect the capillaries and the cable 18 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 19 Re install the mixing chamber to its hol
131. put connector 5 Remove the board Place it on an ESD kit The RFI spring plate sitting on the board connectors is very sharp Be careful not to cut yourself when removing it from the old board and sliding it onto the new board 6 In most cases the RFI spring plate Radio Frequency Interference spring plat prevents radio emissions from the instrument to ambient remains on the interface connectors of the board Carefully remove the spring plate from the old board and slide onto the new board before installing the new board in the pump the RFI spring plate is NOT part of an exchange board 1200 Series Nano Pump Service Manual 147 3 Repairing the Pump 7 Ifyou have to exchange other parts also continue with that work first 8 On the new board check the switch setting of address switch S1 see Table 39 on page 238 An incorrect switch setting e g TEST BOOT may cause the pump to turn into a basic mode yellow or red flashing status light In such a case turn off the pump re set the address switches and turn on the pump again 9 Install the new board and reconnect the connectors Make sure that the board is fitted correctly in the board recess holes at the rear panel CAUTION Make sure that P21 is not accidentally connected into the position of P16 P17 possible on revision A and B boards This will damage the encoder of pump drive B when turned on 10 Replace the connector screws fa is gt
132. r Reconnect the capillaries and connectors P16 P11 P20 19 Re install the mixing chamber to its holder 20 Re install the flow sensor Reconnect the capillaries and the cable 21 Re install the EMPV Reconnect the capillaries the waste tube and the cable 22 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 167 1200 Series Nano Pump Service Manual 161 3 Repairing the Pump Exchanging the Leak Sensor Frequency Leak messages without leak in the funnel Tools required Screwdriver Pozidriv 1 e Wrench 1 4 inch Wrench 14 mm Wrench 7 mm Wrench5mm Parts required Leak sensor 5061 3356 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 143 2 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 3 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 147 6 Remove both
133. r 0 means that the switch is down and 1 means that the switch is up Table 41 Baud Rate Settings Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 42 Data Bit Settings Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication 1200 Series Nano Pump Service Manual 239 6 Introduction to the Nano Pump Table 43 Parity Settings Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 I Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Forced Cold Start Settings CAUTION Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased 240 If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 44 Forced Cold Start Settings Mode Select 1 2 3 A 5 i 8 seo PR RO a RO o To return to normal operation set switches back to your GPIB or RS 232 configuration s
134. r cords is different and designed to match the wall socket of a particular country or region Never operate your instrumentation from a power outlet that has no ground connection Never use a power cord other than the power cord designed for your region 14 1200 Series Nano Pump Service Manual Installing the Pump 1 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bench Space The nano pump dimensions and weight see Table 1 allow to place the nano pump on almost any laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for the circulation of air and electric connections If the bench should carry a complete Agilent 1200 Series system make sure that the bench is designed to carry the weight of all the modules The pump should be operated in a horizontal position Environment CAUTION Your nano pump will work within specifications at ambient temperatures and relative humidity as described in Table 1 Do not store ship or use your nano pump under conditions where temperature fluctuations could cause condensation within the nano pump Condensation will damage the system electronics If your nano pump was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation Table1 Physical Specif
135. r indicates a not ready condition The nano pump is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a setpoint or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the nano pump has detected an internal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis A flashing yellow status indicator indicates that the module is in its resident mode Call your local service provider for assistance upon observing this error condition A flashing red status indicator indicates a severe error during the startup procedure of the module Call your local service provider for assistance upon observing this error condition Error Messages Indication Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair frit exchange or exchange of consumables required In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument logbook 36 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions E
136. re Limit Exceeded 61 Motor Drive Power 62 Encoder Missing 63 Inlet Valve Missing 64 Electro Magnetic Proportional Valve EMPV Missing 65 Flow sensor missing 66 Unsupported Flow Sensor 6 7 Leak Sensor Missing 68 Servo Restart Failed 69 Pump Head Missing 70 Index Limit 71 Index Adjustment 72 Index Missing 73 Stroke Length 74 1200 Series Nano Pump Service Manual Initialization Failed 75 Wait Time out 76 Testing your Nano Pump 77 Micro Mode Pressure Test 77 Normal Mode Pressure Test 80 Leak Test 85 EMPV Test 96 Evaluating the results 97 Flow Sensor Accuracy Calibration 98 EMPV Cleaning 103 Basic System Troubleshooting 104 System Pressure Abnormally Low 105 System Pressure Abnormally High 106 EMPV failed to initialize micro mode only 107 Unstable column flow and or system pressure 108 Poor peak shape 110 Failure to produce peaks or abnormally small peaks after injection 111 Wandering Detector Baseline 112 User interface displays error messages for specific modules 113 114 3 Repairing the Pump Introduction 117 Simple Replacements 117 Exchanging Internal Parts 117 Cleaning the Nano Pump 118 Using the ESD Strap 118 Overview about the Nano Pump 119 Simple Repair Procedures 120 1200 Series Nano Pump Service Manual Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 121 Removing the Active Inlet Valve 121 Exchanging the Valve Cartridge 122 Replacing the Active Inlet Valve 122 Exch
137. re solvent delivery are accomplished by two pump channels each capable of delivering a maximum of 2 5 ml min flow at up to 400 bar pressure 1200 Series Nano Pump Service Manual 209 6 Introduction to the Nano Pump Each channel consists of an identical independently controlled pump unit Each pump unit includes a pump metering drive assembly and pump head assembly The pamphlet assemblies both consist of two identical chambers pistons and seals plus an active inlet valve and an outlet ball valve The channel flow outputs are initially joined by a low volume pre mixer and are then connected by a capillary coil to a pressure pulse damper The pressure pulse damper also serves as a pressure transducer which sends system pressure information to the user interface This output flow called primary flow is connected to the Electronic Flow Control EFC system The EFC system consists of an Electro Magnetic Proportioning Valve EMPV in series with a Flow Sensor The EMPV is protected from particles in the mobile phase by a solvent filter frit Responding to user entered column flow setpoint the EFC system determines how much of the main flow volume is ultimately delivered to the column The remaining main flow volume which is not required by the column is diverted to waste by the EMPV Under user control the EMPV can also function as a purge valve for purposes of solvent changeover etc In this case the EMPV is totally open and th
138. re the pump mode is set to Normal Access the pump Purge control Set up a purge table which will purge all channels for 5 minutes each at a flow of 2500 ul min Then start the purge When the pump has been turned off for a certain time for example overnight oxygen will re diffuse into the channels between the degasser and the pump It is suggested to purge each channel at 2500 pl min for 1 minute at the beginning of each day 1200 Series Nano Pump Service Manual Table 4 Choice of Priming Solvents for Different Purposes Installing the Pump 1 Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system After an installation second choice Ethanol or Methanol Alternative to Isopropanol if no Isopropanol is available When switching between reverse phase Isopropanol Best solvent to flush air out of the system and normal phase both times To clean the system when using buffers Bidistilled water After a solvent change Bidistilled water After the installation of normal phase seals Hexane P N 0905 1420 5 Isopropanol To clean the capillaries Acetone Best solvent to re dissolve salts Best solvent to re dissolve salts Good wetting properties Best solvent to remove impurities from the capillaries 1200 Series Nano Pump Service Manual 31 1 32 Installing the Pump 1200 Series Nano Pump Service Manual Agilent 1200 Series Nano Pump Service Manual 2 Trou
139. red through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF 1200 Series Nano Pump Service Manual Appendix 8 Declaration of Conformity for HOX2 Filter Declaration of Conformity We herewith infom you that the Holmium Oxide Glass Filter Type Hoya HY 1 Part No 79880 22711 meets the following specification of absorbance maxima positions e n _ Number Accura Banduidih A Irm C T 79853C 1050 Esa Sron i rm 413 5rm isama drm G1314A B C 1100 1200 360 8ren Ld lrn 418 5mm 536 4rn The variation in Measured Wavelength depends on the different Optical Bandwidth Agilent Teckmologies guarantees the trace ability of the specified absorb ance maxima to a National Institute of Standards amp Technology NIST Hohmmnn Oxide Solution Standard vrith a lot to lottolerance of 0 3 rm The wavelength calibration filter built into the Agilent Teckmologies UV VIS detectors is made of this material and meets these specifications E is therefore suitable for vravelength calibration of these detectors within the specified wavelength accuracy of the respective dete ctor over its vravelength range
140. rror Messages Time out 39 Shutdown 40 Remote Time out 41 Synchronization Lost 42 Leak 43 Leak Sensor Open 44 Leak Sensor Short 45 Compensation Sensor Open 46 Compensation Sensor Short 47 Fan Failed 48 Open Cover 49 Restart Without Cover 50 Zero Solvent Counter 51 Pressure Above Upper Limit 52 Pressure Below Lower Limit 53 Pressure Signal Missing 54 Valve Failed 55 Missing Pressure Reading 56 Pump Configuration 57 Valve Fuse 58 Inlet Valve Fuse 59 Temperature Out of Range 60 Temperature Limit Exceeded 61 Motor Drive Power 62 Encoder Missing 63 Inlet Valve Missing 64 Electro Magnetic Proportional Valve EMPV Missing 65 Flow sensor missing 66 Unsupported Flow Sensor 67 Leak Sensor Missing 68 Servo Restart Failed 69 1200 Series Nano Pump Service Manual 2 Troubleshooting and Test Functions Pump Head Missing 70 Index Limit 71 Index Adjustment 72 Index Missing 73 Stroke Length 74 Initialization Failed 75 Wait Time out 76 38 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Time out The time out threshold was exceeded Probable Causes e The analysis was completed successfully and the time out function switched off the nano pump as requested e A not ready condition was present during a sequence or multiple injection run for a period longer than the time out threshold Suggested Actions V Check the logbook for the occurrence and source of a not ready condition Restart
141. rvice Manual 199 5 Identifying Cables BCD Cables One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to General Purpose Connector Wire Color Pin Signal Name BCD Digit G1351 81600 Agilent 1200 Green 1 BCD 5 20 Violet 2 BCD7 80 ST Blue 3 BCD 6 40 LEE Yellow 4 BCD 4 10 Black 5 BCD 0 1 SNA ES Orange 6 BCD3 8 Red 7 BCD2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 not connected 14 not connected 15 5V Low 200 1200 Series Nano Pump Service Manual Identifying Cables 5 Agilent 1200 to 3396 Integrators Connector Pin Pin Signal Name BCD Digit 03396 60560 3392 3 Agilent 1200 1 1 BCD 5 20 Es 2 BCD 7 80 de d 3 3 BCD 6 40 to 4 4 BCD 4 10 i o 5 5 BCDO 1 1e 6 6 BCD3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 1200 Series Nano Pump Service Manual 201 5 Identifying Cables Auxiliary Cable One end of this cable provides a modular plug to be connected to the Agilent 1200 Series vacuum degasser The other end is for general purpose Agilent 1200 Series Degasser to general purposes Connector Color Pin Signal Name G1322 61600 Agilent 1200 White 1 Ground Brown 2 Pressure signal e Green 3 i Yellow 4 Grey 5 DC 5V
142. s Enter Into the box labeled Instr Instruction enter the command TYPE XXXXX Letters and numbers are created using the up and down arrows XXXXX is the 5 character product number of the detector being changed There must be a space between the word TYPE and the product number Examples TYPE G2226A to configue as a WPS To configure the pump as G2226A nano pump requires the correct main board version If you enter the wrong type your module will not be accessible anymore In such a case see Changing the Product Number and Serial Number on page 149 or Using the Control Module G1323B on page 151 for recovering 152 Now press the Execute F8 key Below the box a reply line should then say Reply RA 0000 TYPE XXXXX XXXXX is what you just entered Turn the detector OFF the ON again Turn on should be normal In the Records screen the product column should indicate the module you just entered If an Agilent ChemStation is also connected re boot it now 1200 Series Nano Pump Service Manual Repairing the Pump 3 Replacing the Nano Pump s Firmware The installation of new firmware is required e if anew version solves problems of the currently installed version e if the version of firmware on the new main board NPM after an exchange of the board is older than the one previously installed To upgrade the nano pump s firmware follow the procedures and instructions given on the internet http lsbu mar
143. s attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met 1200 Series Nano Pump Service Manual In This Guide 1200 Series Nano Pump Service Manual Annex A This guide contains information to install the Nano Pump on both a Cerity NDS Professional system stand alone and client server installation Installing the Pump How to install the nano pump Troubleshooting and Test Functions The nano pump s built in troubleshooting and test functions Repairing the Pump Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts Parts and Materials Detailed illustrations and lists for identification of parts and materials Identifying Cables A wide spectrum of customary as well as special cables is shown to keep sampler in contact to different interfaces Introduction to the Nano Pump An introduction to the nano pump instrument overview theor
144. sured values are evaluated automatically A table with the measured pressure drop over the inlet filter the measured flow and pressure offset and the correction factors for all steps are printed You have now the possibility to accept and store all the correction factors and the measured offset accept only the measured offset reset no correction factor or offset are applied 100 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Test evaluation feet i a fer Date 6 25 02 Time 4 3 Calbrat bo soil ei cai Figure 11 Flow sensor accuracy calibration test evaluation Table5 Flow sensor accuracy calibration test evaluation Pressure drop over filter The measured value should not be higher If higher we recommend to than 10 Bar change the filter in front of the EMPV and restart the test again Offset flow This value can be positive or negative If higher or lower replace the and should be between 500 nL min and nano flow sensor 90 nL min 1200 Series Nano Pump Service Manual 101 2 Troubleshooting and Test Functions Table5 Flow sensor accuracy calibration test evaluation continued Offset pressure sensor This value has not to be considered in the evaluation Correction factor Step 1 Between 95 and 105 Ifvalue out of range check the Step 5 Between 80 and 500 restriction capillary for Step 2 4 Continuous trend between damage or blockage and Step 1 and Step 5 rep
145. t of the pump head The pressure produced by piston 2 also closes the outlet ball valve preventing any chamber 2 solvent from back streaming into chamber 1 After a predefined piston 1 stroke length the servo motor is stopped and the active inlet valve is closed The pistons now reverse directions Piston 1 begins its delivery stroke high pressure and piston 2 begins its intake stroke Piston 2 is moving at only half the speed of piston 1 The outlet ball valve is forced open by the pressure generated by piston 1 Piston 1 begins to deliver the volume previously sucked into chamber 1 Because of the 2 1 speed ratio of the pistons half of the solvent flow from chamber 1 is forced out of the pump head continuing into the pump hydraulic path The other half of the flow from chamber 1 simultaneously refills chamber 2 1200 Series Nano Pump Service Manual Introduction to the Nano Pump 6 When piston 1 has completed its delivery stroke the pistons reverse direction and the cycle is repeated To mixing gt chamber Inlet From valve solvent_A gt bottle Ball screw drivi Gear RR Motor with encode Figure 38 Operating Principle of the pump head Table 30 Nano Pump Details Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active Inlet Valve SST gold sapphire ruby ceramic PTFE Outlet Valve SST gold sapphire ruby tantalum Adapter
146. t to use features such as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level is defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e outputs are open collector type inputs outputs wired or technique Table 36 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities
147. tack Table 15 Simple Repair Procedures Procedure Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve on page 121 Exchanging the Outlet Ball Valve Sieve or the Complete Valve on page 124 Installing the Manual Purge Valve on page 126 Installing the Manual Purge Valve on page 126 Exchanging the Pump Seals and Seal Wear in Procedure on page 134 Exchanging the Plungers on page 137 Exchanging the Flow Sensor on page 138 120 Symptom If internally leaking If internally leaking Unstable column flow or system pressure Column flow and system pressure drops from time to time If pump performance indicates seal wear If scratched Extended flow range 100 pl needed Leak on the flow sensor Unstable column flow Flow sensor blocked Notes Pressure ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification A pressure drop of gt 10 bar across the frit 2 5 ml min H20 with purge open indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification Seal life time shorter than normally expected check plungers while changing the seals 1200 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve Frequency If internally leaking backflow To
148. talling the Nano Pump 1 Place the pump on top of the micro sampler Make sure that the two modules are interlocked correctly 2 Connectthe power cable to the power socket at the rear of the pump Do not connect the power cable to power until you have finished the hardware installation of all modules in the stack Configuration switch Slot for interface board Remote n Analog sp oo 00139 CAN Bus TEL VA 220 Hz 60 80 F Uno ie A V 100 120 220 240 78 s 4 s s HP4B Address eC in LUI pese INNI i HERBE o o o oln a ANNNAN U n U U U U n U ry U r U n U r VUUU D Power Plug Security Lever Figure 5 Rear of Nano Pump 3 Connect the free end of the Can bus cable from the micro sampler to one of the Can bus connectors at the rear of the pump 1200 Series Nano Pump Service Manual 25 1 Installing the Pump In an Agilent 1200 Series system the individual modules are connected through CAN cables The Agilent 1200 Series vacuum degasser is an exception A vacuum degasser can be connected via the APG remote connector to the other modules of
149. tegrator on the analog output or in the plot screen in the ChemStation The pressure profile is shown in Figure 8 Step 2 T T T T T T T 0 0 5 1 15 2 25 3 3 5 Time minutes Figure 8 Typical Pressure Test Pressure Profile with IPA The test begins with the initialization of both pumpheads After initialization plungers Al and B1 are both at the top of their stroke Next pump A begins pumping solvent with a flow rate of 510 ul min and stroke of 100 ul The nano pump continues to pump until a system pressure of 390 bar is reached 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Step 2 When the system pressure reaches 390 bar the nano pump switches off The pressure drop from this point onwards should be no more than 2 bar minute For this test only channel A2 is active To test the pressure tightness of the pump component use the leak test see Leak Test on page 85 Positioning the blank nut If a specific component is suspected of causing a system leak place the blank nut immediately before the suspected component then run the pressure test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails Running the Normal Mode Pressure Test Tools required Wrench 1 4 inch Parts and materials Blank nut 01080 83202 required Isopro
150. th a blank nut 01080 83202 Positioning the blank nut on page 81 Set flow for channel A2 to 2500 ul min and flush the degasser channel for about 5 minutes Set flow to 0 ul min 5 Connect the signal cable to the analog output at the rear of the nano pump only if an integrator is used Press Execute to initialize the pressure test 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Once the test is started the nano pump increase the pressure up to approximately 390 bar Afterwards the flow stops and the pressure drop is monitored and displayed on the control module The control module displays a graphical representation of the pressure Evaluating the Results on page 83 describes the evaluation and interpretation of the pressure test results 7 When the test is finished slowly unscrew the blank nut Evaluating the Results The sum of all leaks between the pump and the blank nut will be indicated by a pressure drop of gt 2 bar minute at the plateau Note that small leaks may cause the test to fail but solvent may not be seen leaking from a module Please notice the difference between an errorin the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test where not within the specified limits If the pressure test fails e Ensure all fittings between the pump and the
151. th lint free cloth Ensure all particulate matter is removed Best cleaning results will be achieved by removing all valves see pages 121 124 and the capillary Inject solvent into each chamber Chambers 4 Insert seals into the pump head and press firmly in position 5 Reassemble the pump head assembly see Reassembling the Pump Head Assembly on page 139 Reset the seal wear counter and liquimeter as described in the User Interface documentation 1200 Series Nano Pump Service Manual 135 3 Repairing the Pump CAUTION Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 but it will definitely damage the normal phase application seals 0905 1420 136 Fill a bottle with 100 ml Isopropanol and place the bottle head assembly of the pump head that is supposed to be worn in into the bottle Screw the adapter 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it Connect the restriction capillary 5022 2159 to the outlet of the EMPV Insert its other end into a waste container Turn the system in purge mode and purge the system for 2 minutes with isopropanol at a flow rate of 2 ml min Turn the system to standard mode set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 minutes at this pressure to wear in the seals The pressure can be monitored at your analog output signal with the handheld con
152. the damper V Exchange the CSM board 56 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Pump Configuration At switch on the pump has recognized a new pump configuration The nano pump is assigned its configuration at the factory If the active inlet valve and pump encoder of channel B are disconnected and the nano pump is rebooted the error message is generated However the nano pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable Causes e Active inlet valve and pump encoder of channel B disconnected Suggested Actions Y Reconnect the active inlet valve and pump encoder of channel B 1200 Series Nano Pump Service Manual 57 2 58 Troubleshooting and Test Functions Valve Fuse Valve Fuse 0 Channels Al and A2 Valve Fuse 1 Channels B1 and B2 One of the solvent selection valves in the nano pump has drawn excessive current causing the selection valve electronic fuse to open Probable Causes e Defective solvent selection valve e Defective connection cable front panel to CSM board e Defective CSM board Suggested Actions V Restart the nano pump If the error message appears again exchange the solvent selection valve V Exchange the connection cable V Exchange the CSM board 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Inlet Valve Fuse Inlet Valve Fuse 0 Pump chann
153. the stack The AUX output allows the user to monitor the vacuum level in the degasser chamber An Agilent 1200 Series control module can be connected to the CAN bus at any of the modules in the system except for the degasser The Agilent ChemStation can be connected to the system through one GPIB or LAN requires the installation of a LAN board cable at any of the modules except for the degasser preferably at the detector MUST for the DAD For more information about connecting the control module or Agilent ChemStation refer to the respective user manual For connecting the Agilent 1200 Series equipment to non Agilent 1200 Series equipment see Introduction to the Nano Pump on page 208 4 Connect the pre terminated end of the pump to sampler capillary G1375 87322 or G1375 87323 to the flow sensor outlet of the pump Connect the other end of this capillary to port 1 of the sampler injection valve Carefully route all capillaries so that they are not crushed or broken by module front covers Avoid excessive bending 5 Connectthe 1 8 inch plastic EMPV waste tube to the barbed waste fitting of the EMPV Route the waste tube to an appropriate waste container The pump was shipped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch on page 237 To disconnect a module from line unplug the power cord The power supply still uses some power even if the power switch on the front
154. tive Action Loose or leaky fitting Tighten the fitting or exchange the capillary Untight EMPV Run the EMPV test Damaged pump seals or plungers Run the leak test to confirm the leak High flow sensor offset Run the flow sensor accuracy calibration and correct the flow sensor offset Potential Cause Autosampler Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Needle seat Exchange the needle seat Rotor seal injection valve Exchange the rotor seal Damaged metering seal or plunger Exchange the metering seal Check the plunger for scratches Exchange the plunger if required 1200 Series Nano Pump Service Manual 79 2 80 Troubleshooting and Test Functions Normal Mode Pressure Test Pressure bar Step 1 Description The pressure test is a quick built in test designed to demonstrate the pressure tightness of the system The test should be used when problems with leaks are suspected or after maintenance of flow path components e g pump seals injection seal to prove tightness up to 400 bar The test involves monitoring the pressure profile as the nano pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness of the system The injection device outlet port 6 is blocked with a blank nut and then the test is run using isopropyl alcohol IPA while monitoring the pressure profile using an in
155. to flow sensor capillary on the flow sensor inlet Disconnect the flow sensor to sampler capillary on the flow sensor outlet Unscrew the flow sensor Install the new flow sensor Reconnect the EMPV to flow sensor capillary on the flow sensor inlet Connect the flow sensor accuracy calibration capillary G2226 67300 on the flow sensor outlet Run the flow sensor accuracy calibration from the ChemStation Follow the step by step procedure At the end of the procedure disconnect the flow sensor accuracy calibration capillary 10 Reconnect the flow sensor to sampler capillary on the flow sensor outlet 138 1200 Series Nano Pump Service Manual Repairing the Pump 3 Reassembling the Pump Head Assembly Tools required 1 Place the support rings on the plunger housing plungers not installed and snap the pump head and plunger housing 4 mm hexagonal key together PTFE lubricant 79841 65501 3 mm hexagonal key Pump head Support ring plunger housing 2 Tighten the lock screw 3 Carefully insert the plungers into the pump head assembly and press them completely into the seals Plunger l Lock screw ny Pump head rs assembly 1200 Series Nano Pump Service Manual 139 3 Repairing the Pump 4 Slide the pump head assembly onto the pump drive Apply a small amount of pump head grease to the pumphead Tighten screws stepwise with increasing torque
156. to the syringe 2 Pour intended analytical solvents into the solvent bottles and install the bottles on the descibed solvent channels Install Isopropanol on channels which will not be used right away 3 Put a paper towel over the leak sensor in the pump leak tray 4 Disconnect the channel A solvent tube from the A1 port of the pump solvent selection valve 1200 Series Nano Pump Service Manual 29 1 Installing the Pump Liquid may drip from the disconnected solvent tube Make sure to follow appropriate safety precautions 30 Connect the end of the solvent tube to the syringe adapter Slowly draw a syringe volume 20ml from the solvent tube Disconnect the solvent tube from the syringe adapter and reconnect the tube to the A1 port of the solvent selection valve Eject the syringe contents into an appropriate waste container 7 Repeat steps 4 to 6 for the three remaining solvent channels 8 When all 4 channels are manually primed remove the paper towel from the pump leak tray Make sure that the pump leak sensor is dry before turning on the pump Purging the Pump 1 Make sure that the 1 8 inch plastic waste tube is tightly connected to the barbed waste fitting of the pump EMPV and routed to an appropriate waste container Turn on the LC System All system parameters should be set to default The degasser should also be turned on at this time Initialize the system Then access the pump controls and make su
157. total time approx 5 min REGA aa loc ee 1 Connect bottle with aqueous 41 and organic solvent B1 2 Release system pressure __ 1200 Series Nano Pump Service Manual 103 2 Troubleshooting and Test Functions Basic System Troubleshooting 104 In this troubleshooting guide you will find a Possible Causes Suggested Actions approach to troubleshooting and correcting certain capillary LC system problems The problems are categorized by the following symptoms System Pressure Abnormally Low 105 System Pressure Abnormally High 106 EMPV failed to initialize micro mode only 107 Unstable column flow and or system pressure 108 Poor peak shape 110 Failure to produce peaks or abnormally small peaks after injection 111 Wandering Detector Baseline 112 User interface displays error messages for specific modules 113 This troubleshooting guide deals with systemic problems of the capillary LC For detailed diagnostic troubleshooting and repair information on specific LC modules status indicators error messages diagnostic tests etc refer to the Reference Manual supplied with the specific LC module 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 System Pressure Abnormally Low The current system pressure is significantly below the typical system pressure produced by this method with this column Table 6 System Pressure Abnormally Low Possible causes and actions Possible Causes Suggest
158. troller Chemstation or any other controlling device connected to your pump Turn OFF the pump slowly disconnect the restriction capillary from the EMPV to release the pressure from the system Reconnect the capillary going to the flow sensor and the connecting tube from solvent selection valve to the AIV Rinse your system with the solvent used for your next application 1200 Series Nano Pump Service Manual Exchanging the Plungers Repairing the Pump 3 When required When scratched Tools required 3 mm hexagonal key 4 mm hexagonal key Parts required Plunger 5063 6586 1 Disassemble the pump head assembly see Removing and Disassembling the Pump Head Assembly on page 132 2 Check the plunger surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace plunger if scratched Plunger surface 3 Reassemble the pump head assembly see Reassembling the Pump Head Assembly on page 139 1200 Series Nano Pump Service Manual 137 3 Repairing the Pump Exchanging the Flow Sensor Frequency Leak on the flow sensor Unstable column flow Flow sensor blocked Tools required 8710 2412 2 5 mm hex key 8710 1534 4 mm open wrench Parts required G1376 68704 Nanoflow sensor uy Oo 1 F WwW DB G2226 67300 Flow sensor accuracy calibration capillary 8000 mm 25 um G2226 67300 Turn off the pump Disconnect the EMPV
159. tronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use an ESD protection when handling electronic boards and components 118 Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 12 Using the ESD Strap 1200 Series Nano Pump Service Manual Overview about the Nano Pump Repairing the Pump 3 Figure 13 shows the main assemblies of the nano pump The pump heads and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacing internal parts will require removing the module from its stack and to open the top cover 8 A JZ a i LL f 4 LZ 10 PNN a 4 SS a A 5 13 Figure 13 Table 14 Main Assemblies 1 Pumpdrive A 2 Damper 3 EMPV 4 Outlet Ball Valve A 5 Pumphead A 6 Solvent Selection Valve 7 Leak Sensor Overview of Repair Procedures Main Board Power Supply Fan Flow Sensor Pumphead B Active Inlet Valve B 1200 Series Nano Pump Service Manual 119 3 Repairing the Pump Simple Repair Procedures The procedures described in this section can be done with the nano pump in place in the system s
160. ual Appendix 8 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1200 Series Nano Pump Service Manual 253 8 Appendix Radio Interference Never use cables other than the ones supplied by Aligent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 254 1200 Series Nano Pump Service Manual Appendix 8 Sound Emission Manufacturer s Declaratio
161. ughout the system especially in the following areas All micro sampler valve ports b Column inlet and outlet Flow cell inlet capillary at the capillary cell body junction Refer to chapter 3 for information on connecting capillaries Make sure the capillary connections are correctly made throughout the system especially in the following areas All micro sampler valve ports Column inlet and outlet Flow cell inlet capillary at the capillary cell body junction Refer to chapter 3 for advice on diagnosing an internally broken capillary Check capillaries for an internal break particularly the needle seat capillary the sampler valve to column capillary and the flow cell inlet capillary At very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see Capillaries which have been crushed by module covers are often broken internally and may show no external evidence of a break 110 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Failure to produce peaks or abnormally small peaks after injection There are no peaks or the peak size is significantly below the typical peak size for this method with this column Table 11 Actions Failure to produce peaks or abnormally small peaks after injection Possible Causes and Suggested Possible Causes Suggested Actions Notes A leak in a samp
162. uired V Exchange the pump drive assembly 1200 Series Nano Pump Service Manual 71 2 72 Troubleshooting and Test Functions Index Adjustment Index Adjustment Pump channel A B Index Adjustment Pump channel B The encoder index position in the nano pump is out of adjustment During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable Causes e Irregular or sticking drive movement Defective pump drive assembly Suggested Actions V Remove the pump head and examine the seals plungers and internal components for signs of wear contamination or damage Exchange components as required V Exchange the pump drive assembly 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Index Missing Index Missing Pump channel A B Index Missing Pump channel B The encoder index position in the nano pump was not found during initialization During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable Causes e Disconnected or defective encoder cable
163. ure plots shown below are examples only The plots may vary depending on the type and degree of leakage 1200 Series Nano Pump Service Manual 89 2 90 Troubleshooting and Test Functions No pressure increase or minimum pressure of plateau 1 not reached Potential Cause Corrective Action Pump not running Wrong solvent line connections to solvent selection valve Loose or leaky fittings Large leaks visible at the pump seals Large leaks visible at active inlet valve outlet valve Check the logbook for error messages Ensure the solvent lines from the degasser to the solvent selection valve are connected correctly Ensure all fittings are tight or exchange capillary Exchange the pump seals Ensure the leaky components are installed tightly Exchange the component if required Pressure limit not reached but plateaus horizontal or positive Potential Cause Corrective Action Degasser and pump channels A and or B not flushed sufficiently air in the channels Wrong solvent Purge the degasser and pump channels thoroughly with isopropanol under pressure use the restriction capillary Install isopropanol Purge the degasser and pump channels thoroughly 1200 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 AII plateaus negative Potential Cause T T T T T T 15 2 25 3 36 4 Corrective Action Loose or leaky fittings
164. ure tightness of the system The test should be used when problems with leaks are suspected or after maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 400 bar The test involves monitoring the pressure profile as the nano pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness of the system Normal Mode Pressure Test on page 80 Leak Test The leak test is a diagnostic test designed to determine the pressure tightness of the nano pump Especially when a problem with the pistons the AIV or the OBV is suspected use this test to help troubleshoot the nano pump and its pumping performance Leak Test on page 85 EMPV Test The test is designed to verify the performance of the EMPV The test must always be done when the EMPV valve is exchanged The test should also be done if column flow stability problems occurs in micro mode see The EMPV test is not a substitute for the leak test or pressure test The leak and pressure tests should also be done when leaks within the pump heads might be the problem EMPV Test on page 96 Flow Sensor Accuracy Calibration The flow sensor accuracy calibration test is designed to calibrate the flow accuracy and to compensate the electronic offset of the nano flow sensor This test should be done if high flow accuracy is needed at flow rates lower than 500 nl min
165. ve holder 4 Remove the plastic cap with the gold seal from the purge valve 5 Using a pair of tweezers or a toothpick to remove the frit Valve body PTFE frit J Gold seal l ld Plastic cap Figure 19 Purge Valve Parts 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Replace the cap with the gold seal Before reinstallation always check the gold seal A deformed seal should be exchanged 8 Insert the purge valve into the purge valve holder and locate the pump outlet and the waste outlet as shown below 9 Tighten the purge valve reconnect outlet capillary and waste tubing When fused silica capillaries are used use the 4 mm wrench together with the torque tool to avoid overtightening 1200 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the EMPV Assembly Frequency If valve internally leaking If unstable column flow in micro mode If column flow and system pressure drops from time to time Tools required Wrench 1 4 inch Wrench 14 mm Wrench 7 16 inch Parts required EMPV assembly G1361 60000 Exchanging the complete EMPV 1 Turn the pump off 2 Remove the pump from the stack and remove the top cover and foam 3 Using a 1 4 inch wrench disconnect the capillaries going to the flow sensor coming from the filter Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure Disconnect the EMPV connector P
166. vent selection valve 130 1200 Series Nano Pump Service Manual Repairing the Pump 3 2 Usinga Pozidriv screwdriver 1 loosen the holding screws of the valves 3 Pull the valve module out of its connector 4 Hold the two plastic bodies of the valves and pull the two solvent selection valves apart 5 Exchange the defective solvent selection valve Press the exchanged valve new half together with the properly working old half 6 Connect the valve module to its electrical connectors and fix the assembly with the two holding screws 7 Reinstall solvent tubes and the active inlet valve connection tubes 1200 Series Nano Pump Service Manual 131 3 Repairing the Pump Removing and Disassembling the Pump Head Assembly CAUTION drive Never start the pump when the pump head is removed This may damage the pump When required Exchanging the seals Exchanging the plungers Exchanging seals of the seal wash option Tools required Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key Preparations for this procedure Switch off nano pump at power switch Remove the front cover to have access to the pump mechanics 1 Disconnect the capillary at the pumphead adapter and the tube at the active inlet valve Beware of leaking solvents Disconnect the active inlet valve cable plug Active inlet valve to mixer Plug 132 1200 Series Nano Pump Service Manual Repairing the Pump 3 1 Using
167. without R 5 Turn the detector OFF 6 Change the 8 bit Configuration Switch back to default settings see Setting the 8 bit Configuration Switch on page 237 7 Turn the detector ON again The Maintenance screen should display the correct type for this module Using the Control Module G1323B Conncet the control module to the detector Turn ON the detector 2 On the control module press System F5 then Records F4 Using the up down arrows make sure that the detector is highlighted 3 Press FW Update F5 then m This will display a box which says Update Enter Serial 4 Press Enter This will display the box labeled Serial 1200 Series Nano Pump Service Manual 151 3 Repairing the Pump Letters and numbers are created using teh up and down arrows Into the box labeled Serial entere the 10 character serial number for the detector When the the 10 character serial number is enetered press Enter to highligh the complete serial number Then press Done F6 Turn the detector OFF then On again The Records Scrreen should be display the correct serial number for this module If an Agilent ChemStation is also connected restart the Agilent ChemStation now as well To change the product number go to the System screen 1 2 3 4 Press Tests F1 and select the detector being changed and press Enter While in the Tests screen press m m m dot m From the box now displayed select the Command and pres
168. xchanging the Outlet Ball Valve Sieve or the Complete Valve 124 Installing the Manual Purge Valve 126 Exchanging the Purge Valve Frit or the Complete Manual Purge Valve 127 Exchanging the EMPV Assembly 129 Exchanging the Solvent Selection Valve 130 Removing and Disassembling the Pump Head Assembly 132 Exchanging the Pump Seals and Seal Wear in Procedure 134 Exchanging the Plungers 137 Exchanging the Flow Sensor 138 Reassembling the Pump Head Assembly 139 Exchanging the Optional Interface Board 141 Exchanging Internal Parts 142 Removing the Top Cover and Foam 143 Exchanging the Nano Pump Main Board NPM Board 146 Exchanging the Damper 154 Exchanging the Fan 156 Exchanging a Pump Drive 158 Exchanging the Power Supply 160 Exchanging the Leak Sensor 162 ott Agilent Technologies 115 3 Repairing the Pump Exchanging Status Light Pipe 165 Assembling the Main Cover 166 Replacing the Top Cover and Foam 167 This chapter includes Instruction on how to repair the pump 116 1200 Series Nano Pump Service Manual Introduction Repairing the Pump 3 Simple Replacements The nano pump is designed for easy repair The most frequent repairs such as plunger seal change and filter frit change can be done with the nano pump in place in the system stack These repairs are described in Simple Repair Procedures on page 120 When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures
169. y of operation external communication and internal connectors Specifications Performance specifications of the nano pump Appendix This chapter contains safety information For any information about running and optimizing your nano pump please refer to the nano pump user manual 1200 Series Nano Pump Service Manual Contents 1 Installing the Pump Site Requirements 14 Power Consideration 14 Power Cords 14 Bench Space 15 Environment 15 Unpacking the Nano Pump 17 Optimizing the Stack Configuration 19 Installing the Nano Pump 24 Installing the Nano Pump 25 Install the Degasser G1379B 27 Install the Solvent Cabinet 27 Get the System Ready for the First Injection 29 Manually Pirming the Solvent Channels 29 Purging the Pump 30 2 Troubleshooting and Test Functions Status Indicators 35 Power Supply Indicator 35 Instrument Status Indicator 36 Error Messages Indication 36 Error Messages 37 Time out 39 Shutdown 40 Remote Time out 41 1200 Series Nano Pump Service Manual Synchronization Lost 42 Leak 43 Leak Sensor Open 44 Leak Sensor Short 45 Compensation Sensor Open 46 Compensation Sensor Short 47 Fan Failed 48 Open Cover 49 Restart Without Cover 50 Zero Solvent Counter 51 Pressure Above Upper Limit 52 Pressure Below Lower Limit 53 Pressure Signal Missing 54 Valve Failed 55 Missing Pressure Reading 56 Pump Configuration 57 Valve Fuse 58 Inlet Valve Fuse 59 Temperature Out of Range 60 Temperatu
170. y precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Aligent Technologies assumes no liability for the customer s failure to comply with these requirements General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards This instrument is designed and certified as a general purpose laboratory instrument for research and routine application only It is not certified for in vitro or medical applications Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the speci

Agilent 1200 Series Nano Pump

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