Home

Hemolyzer - Index of

1. EE 51 5 25 Service RE 52 5 2 6 Aun Mn 52 3 2 7 Service Testing Menu ssiri ei 55 5 2 8 IPIE 55 5 2 9 DIMITRI T 22 5 2 10 Display and Keyboard 56 3 2 11 Stress C bekennen ern nee Free onen ernennen 56 5 2 12 Miscellaneous Setinp scr t ete cedet e RETI PUR E E Ce otio P TRUE do de ated 57 213 Multi user Rescue Code iie rer ERE E 57 6 SERVICE OPERATION P 58 6 1 OPENING THE INSTRUMENT e eU eher oe EIER RU REI IP RR 58 6 2 MONOCHROME DISPLAY ADAPTER EMULATION eene eene nennen 58 6 3 KEY BIOS SETTINGS FOR CORRECT OPERATION eee en eren ennemi enne innen ens 59 6 4 CHECKING THE BIOS SETUP irte ttr EU I FORO I RE REOR TUE E EO POR REED RES 60 6 5 BIOS DESERIPTION M M 60 6 6 DOS FUNCTIONS ON THE INSTRUMENT sesser Ee KENGE epa vene said 62 7 UN COQU DB ONIS COLO D o 63 7 1 ERROR CODES 64 TAA Softw re SyStem eFFOES ere ere DERE IC ge 64 7 1 2 Pneumatic m c Soe 64 7 1 3 Measure errors 65 7 1 4 66 7 1
2. lt gt 167 lt HT gt O lt LF gt P21 HT 8 2 lt HT gt O lt LF gt P22 lt HT gt 5 3 lt HT gt O lt LF gt ETX gt 7 C lt EOT gt Receiver acknowledges DATA transmission and requests for RBC package lt ACK gt RB Hemolyzer 3 sends RBC package lt SOH gt CR lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt CHN lt HT gt 256 lt LF gt 9 lt HT gt 16 lt HT gt 26 lt HT gt 1 lt HT gt 0 lt ETX gt F2 lt EOT gt Receiver acknowledges RBC transmission and requests for WBC package lt ACK gt WC Hemolyzer 3 sends WBC package lt SOH gt DW lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt CHN lt HT gt 256 lt LF gt 0 lt HT gt 15 lt HT gt 84 lt HT gt 5 lt HT gt 3 lt ETX gt 69 lt EOT gt Receiver acknowledges WBC transmission and closes the link lt ACK gt lt SPACE gt D It is possible to request for PLT histogram but it is not shown in this example 90 Hemolyzer 3 10 2 7 Serial Protocol 2 20 2 23 This version utilizes a bit upgraded header information lt SOH gt BD lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt NAME lt HT gt JOE SMITH lt LF
3. here 32 4 1 4 Fluid transfer from MIX chamber to WBC chamber eene 33 4 1 5 34 4 1 6 lm UEM 35 4 1 7 Aperture priming prOCESS p 36 4 1 8 WBC chamber draining process esee eene nnne nenne enne ener nennen trennen nennen 37 4 1 9 RBC chamber draining process essen nennen 38 4 1 10 LOI TTA BIDDER 39 4 2 MAIN FUNCTIONS OF FLUIDIC SYSTEM innen inneren tenen ens 40 4 2 1 Ho nngrmo 40 4 2 2 La E 40 4 2 3 Measurement Cy6le 41 4 2 4 Ay 47 4 2 5 eu Amm 48 4 2 6 gene 48 4 2 7 48 Hemolyzer 3 5 CHECKING THE PROPER OPERATION cusssssossossorsnssonsnnsnnsnnsnnssnssnnsnnsnnsnnsnnssnssnssnnsonsnnsnnsnnsnnsnnnsnne 49 5 1 49 5 1 1 Self test einen mehren 49 Dob de Normal range of Self Test parameters esee eene nennen eren eene enne 50 3 4 3 Troubleshooting Guide for Self test esee 50 2 2 SERVICE MEN 51 5 2 1 Entering to Service Ment iie e aee phone 51 2 2 2 Main Service m G 51 3 2 3 Edit service Contact 51 5 2 4 Device
4. o 0 00100 C Q WBC clogging 11 0x00800 coincidence is too high Linearity error 14 0x04000 B WBC Blank is high or WBC blank 15 0x08000 H HGB Blank is high or no HGB blank 16 0x10000 E No WBC three part The detailed description can be found in the User s Manual Service Manual 85 The markers are given in histogram channel between 0 and 255 where 0 means that the marker could not be found 3 The parameter IDs in Hemolyzer 3 are P01 WBC 10 2 RBC 10 HGB g l P04 96 MCV fl pg 7 MCHC g l PLT 10 96 P10 MPV fl P11 PDWsd fl P12 PDWov 96 P13 RDWsd fl P14 RDWcv 96 P15 LYM 10 P16 MON 10 P17 GRA 10 P18 LYM 96 P19 MON P20 GRA P21 RBCtime sec P22 WBCtime sec The parameter value is always 4 characters wide spaces from left added if necessary It can be 9999 if the value could not be displayed in 4 digits or if the value could not be calculated because of an error The meaning of the parameter flags Flag Displayed Meaning 0 Value correct 1 Value high more than upper limit 2 Value low less than lower limit 3 Value is unreliable 4 E Value not given because of error Value is 5 Value cannot be calculated There is no value
5. RBC WBC and PLT package The format of the histogram packages are the same they differ in type descriptor only The package contains the sample data to identify the package and the histogram SNO lt HT gt 152 lt LF gt internal identifier DATE lt HT gt 19980715 lt LF gt date of measurement YYYYMMDD TIME lt HT gt 114500 lt LF gt time of measurement HHMMSS PID lt HT gt 2 lt LF gt patient identifier 0 9999 CHN lt HT gt 256 lt LF gt number of histogram channels 256 9 lt HT gt value of first histogram channel 1 lt HT gt value of 255 histogram channel 0 value of the last histogram channel 86 Hemolyzer 3 XML record descriptor package Each time a communication is initialized Hemolyzer 3 will send an XML file in ASCII mode receiver application should be aware of this by saving this XML file for later use This package contains the description of the FULL record format FULL record format means transmission of a record stored in Hemolyzer 3 as is in binary format Data sent in an F package should be interpreted according to the description in the XML file below Would an F package arrive without an X package received earlier receiver application can ask for the XML descriptor by sending a request for an X package See WBC RBC PLT histogram package request XML file sent by Hemolyzer 3 lt DB_Struct_DAT gt lt Header gt
6. Open Service Manual 37 4 1 8 WBC chamber draining process The WBC chamber draining is performed via V3 On and V4 On valves by the pump while the 5 must be off The WBC chamber draining is always executed without using the puffer reservoir So other tasks like RBC chamber draining can be performed parallel with this operation 38 Hemolyzer 3 4 1 9 RBC chamber draining process The RBC chamber is drained by the vacuum in the puffer reservoir through V1 On valve V2 must be off This vacuum is made and maintenated by the peristaltic pump During the draining the pump can perform either other task like WBC chamber draining or needle wash or can help the draining of the RBC chamber Service Manual 39 4 1 10 Cleaner priming process The cleaner solution is primed by vacuum in the following way V7 On Cleaner Detector V6 On back side of the RBC aperture back side of the WBC aperture V2 On V3 On V4 On and the peristaltic pump The 1 and V5 valves are off The flow of the cleaner is controlled by the vacuum generated in the puffer reservoir before the start of the priming After the priming the remaining cleaner between the cleaner detector and V6 approx 1 ml can be dosed into the mix chamber Stepper Motor INAL I way Valve 2 3 40 Hemolyzer 3 4 2 Main functions of fluidic system 4 2 1 Initialization Fluidic initialization process performs the following st
7. 3 5 2 5 2 0 1 5 mm sizes e Pocket digital multimeter e Diagonal Cutter plier e Nipper 97
8. Field name Heaa type char length 9 gt lt Field name Modified type DateTime length 1 gt Field name Length type DWORD length 1 gt Field name Order type BYTE length 1 gt Field name Dummy1 type BYTE length 1 gt Field name Dummy2 type WORD length 1 gt lt Field name Capacity type WORD length 1 gt Field name RecLen type WORD length 1 gt lt Field name Dummy3 type BYTE length 7 gt lt Header gt lt Record gt lt Field name RecNo type WORD length 1 path Measures Measure alias RecNo gt lt Field name TimeStamp type DateTime length 1 path Measures Measure alias Date gt Field type WORD length 1 path Measures Measure alias OpID gt Field name SamplelD type char length 8 path Measures Measure alias SID gt Field name PatientID type char length 20 path Patient alias PID gt lt Field name Name type char length 32 path Patient alias Name gt lt Field name BirthDate type DateTime length 1 path Patient alias Birth gt Field name Sex type BYTE length 1 path Patient alias Sex extID Sex gt Field name Doctor type char length 16 path Measures Measure alias Doctor gt Field name LimitsLow type float length 23 path Measures Measure alias field_LimitLow incr 1 gt Field name LimitsHigh type float length 23 path Measures Measure
9. 2 bytes WORD PrVMaxR NA 2 bytes WORD PrVMinW2 2 bytes WORD PrVMaxW2 2 bytes 1 byte char ClogReport 29 LE 29 bytes Total 1240 bytes 92 Field RecNo TimeStamp SamplelD PatientlD Name BirthDate Sex Doctor LimitsLow LimitsHigh PatTypeName VetMode HistoRBC HistoPLT HistoWBC PLTIo PLThi RBClo WBClo LYMhi GRAlo Param Flag Warning Lyse Lyse_2 Options PrVMinW PrVMaxw PrVMinR PrVMaxR PrVMinW2 PrVMaxW2 Age ClogReport Data map of TmeasData inside an F package explanation Internal Record ID Timestamp of sample Operator ID Important in multiuser mode only Sample ID Patient ID Patient name Birth date of patient Gender of patient Doctor s name Array of Low limits for parameters 23 values Array of High limits for parameters 23 values Species name Internal ID of species Array of RBC histogram 256 values Array of PLT histogram 256 values Array of WBC histogram 256 values Lower threshold of PLT graph Upper threshold of PLT graph Lower threshold of RBC graph Lower threshold of WBC graph Upper threshold of LYMs on WBC graph Lower threshold of GRAs on WBC graph Array of measured values 23 elements Array of flags of measured values 23 elements Binary representation of measurement flags Volume of lyse reagent used for sample ml Volume of lyse reagent used for sample ml Options set for sample Probe voltage min for WBC
10. Probe voltage max for WBC Probe voltage min for RBC Probe voltage max for RBC Probe voltage min for WBC2 Probe voltage max for WBC2 Age of patient as filled in PatID dialog Clogging information of sample note 1 2 aa ao aA N NN c c NNN A wd dex po a a an an no no of bytes data type 2 WORD 4 DateTime 2 WORD 8 char 20 char 32 char 4 DateTime 1 BYTE 16 char 23 4 bytes float array 23x4bytes float array 20 char 2 WORD 256 BYTE 256 BYTE 256 BYTE 1 BYTE 1 BYTE 1 BYTE 1 BYTE 1 BYTE 1 BYTE 23x4bytes float array 23 1 bytes BYTE array 8 DWORD 4 float 4 float 2 WORD 2 WORD 2 WORD 2 WORD 2 WORD 2 WORD 2 WORD 1 BYTE 20 char Hemolyzer 3 Service Manual 93 Note 1 WORD data format is stored back to front E g Decimal value 13553 Hexadecimal value 34 F1 Stored as 1 34 Note 2 DateTime is packed binary format It contains YEAR MONTH DAY hour minute in the following format YYYYYYYYYYYY MMMM DDDDD hhhhh mmmmmm e g 11 27 2005 13 37 is stored as 2005 in binary on 12 bits is 0111 1101 0101 11 in binary on 4 bits is 1011 27 in binary on 4 bits is 1 1011 13 in binary on 5 bits is 0 1101 37 in binary on 6 bits is 10 0101 Concatenating these we get 0111 1101 0101 1011 1101 1011 0110 0101 In hexadecimal these become 7D 5B DB 65 and gets stored back to front It is stored as 65 DB 5B 7D Note
11. Unrecoverable Fatal error in Check the proper operation of the pneumatical error mechanical motor modules home end opto occurred n subsystem Possibly switches ribbon cables and the Device s n mechanical jam motor moving Error code s n Status x n Please turn off the instrument and turn it on again 7 1 3 Measure errors Code 1200 Fatal Measlnit error Fatal electronic error Call service Power off the system 1201 Fatal HGB error HGB channel did not Call service Power off the system give a ready signal 66 Hemolyzer 3 7 1 4 Printing errors 1400 Printer error d Printer error Check the connections between the instrument and the printer and check the printer setup in the Settings Printer settings sub menu Printer is out of paper Printer is out of paper Feed the printer with paper Check and error d and there are some the connections between the other printer errors instrument and the printer and check the printer setup in the Settings Printer settings sub menu 1402 Printer is out of paper Printer is out of Feed the printer with paper paper 1403 Printer was not set up The selected printer Modify the printer setup in the correctly type does not match Settings Printer settings sub menu to the printer large over the margin Settings Printer settings sub menu large over the margin Settings Printer settings sub menu small over the margin Settings P
12. Control LEDs Control LEDs Service Manual 23 2 2 5 Micro Dilutor Micro dilutor is taking the precise sample 25 or 50 into the sampling needle It includes a stepper motor a motor opto board and the micro syringe Connection to Valve Connection to sampling needle Connection to PPB Motor opto board Flexible clutch Stepper motor 2 2 6 Puffer reservoir The glass puffer reservoir is directly connected to the pressure sensor During measurement there is no pump activity so the puffer reservoir maintains measuring vacuum stable The instrument measures atmospheric pressure and adjusts measuring vacuum according to it 2 2 7 Pump Pump generates regulated vacuum and drains the fluidic system It is connected to the PPB and it has its own driver circuit Darlington If the tube of the peristaltic pump becomes worn it can be broken causing Pressure error It is recommended to check the state of the tube and replace it every 2 years or after 20 000 measurements Always replace the peristaltic pump tube to the same PharMed type with the same length For servicing the tube of the pump open the peristaltic pump from its top see picture and remove the tube together with the white plastic side wall see picture J In case of damaged tubes it can be replaced by a new one by opening the two metal locks located at the two ends of the tube see picture 24 3 2 3 Assemb
13. 2 8 Bleaching of Fluidic System niii ret t a kan 75 9 SPARE PARTS 76 Service Manual 5 10 APPENDICES 80 10 WARNING RLAGS 80 10 2 SERIAL COMMUNICATION 82 10 2 1 General Description een ni 82 10 2 2 Format of Packages nen un nalen an nn 83 10 2 3 Format of Acknowledge of the 83 10 2 4 Detailed Description Of 83 10 2 5 Serial Protocol 1 0 u a 88 10 2 6 Serial Protocol Tasse a 89 10 2 7 Serial Protocol 2 20 D238 90 10 2 8 PLOLO COL E 91 10 3 HEMOLYZER 3 CABLING t 95 10 4 HEMOLYZER 3 TUBING SCHEMATICS ccsscceescecssecesneeceaeceseeecsaeceeneecsaecesneecsaeceeneecsaeceeeecsaeceeneesnaeeeseees 96 10 5 RECOMMENDED KIT OF TOOLS 97 Hemolyzer 3 Service Manual 1 INTRODUCTION Although Hemolyzer 3 is a member of Analyticons Hemolyzer family it has special and different characteristics compared to other junior family members We issue a dedicated Service Manual for this instrument information herein applies for Hemolyzer 3 To be
14. 3 Character strings are stored in character arrays space not used within a string is always filled with 05 String to store George Gets stored on 20 chracters as G e 00000000000000 Note 4 Sex is decoded as Sex 0 Not specified Sex 1 Male Sex 2 Female Note 5 Float numbers are stored on 4 bytes according to the IEEE 745 standard Note 6 Vetmode stores the internal database identifier for the record It is of no importance here Note 7 Histogram representation Each histogram is stored in an array of 256 bytes Each byte represents the histogram value at the given point as a value from 0 to 255 lowest PLT volume Actual volume PLTlo x 50 fl 256 PLThi highest PLT volume Actual volume PLThi x 50 fl 256 RBClo lowest RBC volume Actual volume RBClo x 200 fl 256 WBClo lowest WBC volume Actual volume WBClo x 400 fl 256 LYMhi highest LYM volume Actual volume LYMhi x 400 fl 256 GRAlo lowest GRA volume Actual volume GRAlo x 400 fl 256 94 Hemolyzer 3 Full scale and resolution of histograms PLT 50 fl 1 fl x 50 256 RBC 200 fl 1 fl 200 256 WBC 400 fl 1 fl 400 256 Note 8 Flags for individual parameters See page 4 remark 4 Note 9 Warning flags see page 3 remark 1 Note 10 Options flag Options field interpretation Option mask Displayed Meaning 0x0001 PREDIL sample was run in pre diluted mode 0x0002 RBCEXT sampl
15. 3 Hematology Analyzers consist of the following mechanic and fluidic parts 1 WP Sampling needle Washing head H amp V moving unit Micro Dilutor Dilutor Chambers Cell counter probes Puffer reservoir 9 Pump 10 Valves 11 Tubing Hemolyzer 3 Fluidic Schematics 49 Stepper Motor 3 way Valve 2 way Valve 1 3 Off amp Closed Off Liquid Detector 2 3 On Open 20 Hemolyzer 3 2 2 1 Sampling needle Sampling needle is assembled in the H amp V moving unit and it makes the sample aspirations Correct setting of sampling needle is necessary and very important see Chapter Adjustments 2 2 2 Washing head Washing head is located at the bottom of the H amp V moving unit and it is for cleaning the outer surface of the sampling needle This washing process is made with diluent reagent and the fluid is drained by the pump The arrows on the picture show the direction of diluent flow during sampling needle washing Clean diluent Pump to waste gt i Clean replace washing head after 10 000 measurements Service Manual 21 2 2 3 H amp V moving unit This unit contains slides to move the sample sampling needle in Horizontal and Vertical directions two stepper motors XYR opto board opto wheel washing head and the sampling needle It moves the needle to the desired position from sampling position to washing head
16. 5 67 7 2 WARNING MESSAGES LE 68 742 1 PRA D nupt NOR 68 7 2 2 Database relating warnings er bee tre ten ING 69 723 Warnings relating to QC 69 7 2 4 Calibration relating warnings eese 69 7 2 5 Hardware relating Warnings 70 7 2 6 Other Warniligs E AO ETNE 70 7 3 POSSIBLE CAUSES QN 71 7 3 1 Contaminated uo esi 71 7 3 2 Bad earth grounding iei ski 71 43 3 Externalelectrical NOISE iis eee 72 7 3 4 Internal n isesources ade K IRIS EINE TENE 72 8 MAINTENANCE OR 74 8 1 WEEKLY MAINTENANCE BY USER 5 eese stesse testa eerta esto seen 74 8 1 1 Cleaning the Washing head nenn 74 8 2 PERIODIC MAINTENANCE BY SERVICE ER EI 74 8 2 1 Check Self test and Device statistics eene nennen rennen nete e 74 8 2 2 Cleaning and Greasing Dilutor 74 6 2 3 Checking and Lubricating Dilutor Piston Tips eese eene 74 8 2 4 Cleaning and Lubricating Needle Moving Mechanics esee 74 8 2 5 Checking and Replacing Washing eene een eene 74 8 2 6 Checking and Replacing Peristaltic Pump 74 8 2 7 Checking condition of ea Lo ree Tue tes iR 75 8
17. 7 2 5 Hardware relating warnings 5400 12V too low The 12V is not Contact the Service Personnel sufficient the measurements are unreliable 5401 12V too low The 12V is not Contact the Service Personnel sufficient the measurements are unreliable Battery voltage too low The battery has been Check Date and Time settings discharged This problem has no effect on measurement Contact Service Personnel 7 2 6 Other warnings Insert software update Software upgrade After pressing the system media will restart The system will restart 5904 This function will delete Delete If you press Jall records will all selected records be deleted If you press it will be not deleted 5906 Now it is safe to turn Shut down process Power off the analyzer instrument off has been finished Now it is time to wipe the Maintenance day You have to perform weekly washing head with a maintenance 5907 damp cloth Make sure it If you want to change the is clean then you proceed maintenance day contact Service Personnel Service Manual 71 7 3 Possible Causes of Noise Generally high count of any particle even if you think it should be low or near zero can be caused by NOISE i e something interferes with measurement The most important thing in these cases to identify the source of NOISE otherwise you cannot protect the system against it NOISE can come from has several sources
18. WBC and PLT packages at acknowledge If serial communication protocol is set to version 3 0 Hemolyzer 3 will send XML and FULL packages Special characters used in the communication Character ASCII code lt SOH gt 1 lt STX gt lt ETX gt lt EOT gt lt ENQ gt lt ACK gt lt HT gt lt LF gt lt NAK gt 21 lt SPACE gt 32 O Service Manual 83 10 2 2 Format of Packages Sent The packages sent by Hemolyzer 3 are always between lt SOH gt and lt EOT gt character and they consist of header data and tail The header consists of a package identifier and a package type descriptor The tail includes the checksum Typical format lt SOH gt MID CMD lt STX gt MESSAGE lt ETX gt CHKSUM lt EOT gt MID Message ID one capital letter between A and 2 CMD Command one capital letter package type descriptor F for INIT D for DATA for RBC W for WBC P for PLT X for XML descriptor F for full record transmission MESSAGE the message consists of ASCII characters between 32 128 CHKSUM two hexadecimal digits of a one byte checksum which contains the lower byte of the sum of characters between the lt SOH gt and lt ETX gt incl those too 10 2 3 Format of Acknowledge of the Receiver The receiver must acknowledge transmission by the following message lt ACK gt CMD MID CMD the type of the next package to sen
19. alias field_LimitHigh incr 1 gt lt Field name PatTypeName type char length 20 path Measures Measure alias PatTypeName gt lt Field name VetMode type WORD length 1 path Measures Measure alias field_VET source PatTypeName gt Field name HistoRBC type BYTE length 256 path Measures Measure field_RBCH alias D extID Histo gt lt Field name HistoPLT type BYTE length 256 path Measures Measure field_PLTH alias extID Histo gt lt Field name HistoWBC type BYTE length 256 path Measures Measure field_WBCH alias D extID Histo gt lt Field type BYTE length 1 path Measures Measure field_PLTH alias PM1 gt lt Field name PLThi type BYTE length path Measures Measure field_PLTH 2 gt lt Field name RBClo type BYTE length 1 path Measures Measure field_RBCH alias RM1 gt lt Field name WBClo type BYTE length 1 path Measures Measure field_WBCH alias WM1 gt lt Field name LYMhi type BYTE length 1 path Measures Measure field_WBCH alias WM2 gt lt Field name GRAlo type BYTE length 1 path Measures Measure field_WBCH alias WM3 gt Field name Param type float length 23 path Measures Measure alias field_P incr 1 extID Param gt Field name Flag type BYTE length 23 path Measures Measure alias field_P alias2 _f incr 1 extID Flag gt Field name Warning type DWORD length 1 path Meas
20. and Sample rotor motors have one combined ribbon cable connection Main Dilutor with two motors and Micro dilutor have separated connectors Valve driver section is based on the valve driver PIC micro controller and three 8 bit powered output shift registers with built in protection diodes and there are two common ribbon cable connections for the 4 valve boards The peristaltic pump has a separated Darlington driver circuit for more reliable operation All the 7 6 for motors 1 for pneumatic microcontroller have 2 LEDs a yellow one and a green one The yellow one indicates motor moving or holding and active valve or pump moving it means current flows into motors valves or pump Service Manual 15 The green one has 3 states e dark after initialization phase error state e blinking communication in progress normal state e on just lighting OK normal state 2 1 8 Opto boards for stepper motors There are five stepper motors in the system Horizontal and Vertical motors which make the movements of the sampling needle the main Dilutor motors 2 which move the syringes and the micro Dilutor motor which drives the sampling phase The stepper motor opto boards make the connections between the motor driver ICs and motors and have opto switches for the motor s home and end positions The actual status of the stepper motor s optos is indicated by two LEDs on each stepper motor opto boards Dilutor and Micro dilutor have i
21. can make the following selections on the Main Menu itself Use the sub menus for other selections 62 Hemolyzer 3 6 6 DOS functions instrument run computer part of the instrument like normal the Service should have a floppy drive with cables see chapter 9 5 and an MS DOS 6 22 operating system boot floppy disk Take off upper cover of instrument Connect an external keyboard Connect the floppy drive to PPB board Floppy CD power connector COMB board FLOPPY connector Put the MS DOS boot floppy into the floppy disk drive After turning on go to the BIOS setup press F2 repeatedly on the keyboard and switch to MDA mode by pressing i Change the Boot Device Priority in the BOOT menu The Removable Devices must be the first in the list Select Removable Devices and move it up by pressing on the keyboard Save the actual settings to CMOS the instrument will restart Exit Saving Changes The instrument will boot from the floppy and the software will be terminated Switch to MDA mode by pressing i Do not forget to restart the instrument and set back Boot Priority list after servicing Hard Drive first Service Manual 63 7 TROUBLESHOOTING The analyzer checks the operations of several mechanic fluidic and electronic parts during measurement The system shows the type of the error on the LCD display if any kind of malfunction is detected The electronic parts ha
22. component failure Check the shielded cable connections as well Sometimes inside out connection hot electrode goes outside as a shield is the problem both ends of amplifier signal cable must be reversed analog signal ribbon cable it picks up noise Check the ribbon cable between the circuit board and the amplifier Maybe it is pinched under some screws or components This may cause trouble and even noise 7 3 4 4 D Pneumatic failures liquid paths that conduct noise into the chamber liquid remains under the chamber in drain tube during measurement the conducting liquid remains inside the drain tube making noise to appear there Check chamber draining path for clogging or salt crystals Check the pump operation Since draining of the chamber goes under pressure control maybe a bad pressure sensor or connection can cause trouble Clean the draining path Do not use alcohol but bleach Replace chamber if necessary liquid remains in the washing inlet at top of the chamber during measurement the conducting liquid remains inside the chamber wash tube making noise to appear The software is not compatible with the mechanics or related valve is bad partly clogged or the tubing is clogged loose lyse path guides in noise during counting if the a liquid in the draining tube is touching lyse reagent in T fitting noise can appear Check the lyse path and the lyse valve as well 74 8 1 8 1 1 8 2 8 2 1
23. input range of the A D converter which is placed on the COMB card There is an offset potentiometer P1 in the third amplifier stage manufacturer sets the correct offset voltage Adjust the offset voltage only in case it is out of the 5mV range DHON signal from the COMB card switches on the LED in the HGB head via a transistor but the Photo Detector in the HGB head is working continuously The other side of the amplifier board contains special connectors for the chambers and the HGB head 2 1 4 Control and Measurement Board COMB with DIMM PC core The compact COMB incorporates a single PC and its environmental functions as well as the specific measurement processing functions in one board PS2 COM USB USB kevboard Speaker gt 4 IDEPROM a connection HVB connection Display connection 4 Floppy connection IDE connection Amplifier connection DIGIO PC system of the COMB board is based on the DIMM PC module which is a credit card size PC with AMD Elan SC520 133 MHz micro controller DIMM PC itself contains 16 or 32Mbyte RAM and same size of FlashDisk that acts like a hard disk DIMM PC module is easily replaceable as it has an open socket it has also a screw for safe fixing COMB card contains single ICs and some drivers protection circuits for the interfaces such as COM1 PS2 USB IDE and Speaker Measurement processing is based on a FPGA circui
24. items name and value which are separated by a lt HT gt character and closed by lt LF gt SNO lt HT gt 152 lt LF gt internal identifier DATE lt HT gt 19980715 lt LF gt date of measurement YYYYMMDD TIME lt HT gt 114500 lt LF gt time of measurement HHMMSS PID lt HT gt 2 lt LF gt patient identifier 0 9999 NAME lt HT gt JOE SMITH lt LF gt patient name max 32 characters MODE lt HT gt 0 lt LF gt patient type JRN lt HT gt O lt LF gt warning bits 32 bit hexadecimal PM1 lt HT gt 12 lt LF gt PLT lower marker PM2 lt HT gt 204 lt LF gt PLT upper marker R RM1 lt HT gt 51 lt LF gt lower marker M1 lt HT gt 23 lt LF gt WBC upper marker IM2 lt HT gt 57 lt LF gt LYM MID marker 13 lt HT gt 92 lt LF gt WBC MID GRA marker PARN lt HT gt 22 lt LF gt number of parameters to send 01 lt gt 6 6 lt HT gt 0 lt LF gt first parameter ID value and flag PO2 lt HT gt 4 29 lt HT gt 0 lt LF gt second parameter ID value and flag gt 22 lt gt 8 2 lt HT gt 0 lt LF gt last parameter ID value and flag Remarks Position and meaning of the warning flags Bit Mask Letter Meaning 0x00001 RBC PLT clogging 0x00008 m RBC PLT Coincidence is too high Linearity error 0x00040 b RBC Blank is high or no RBC blank 0x00080 p PLT blank is high or no PLT blank
25. opto end switches of the H amp V moving unit The top of this block is called HV head and it is shown in the figure below Set the distance to 1 2 mm between the moving carriage and the stable part of the head Loose screws to allow free movement of the timing belt Adjust the opto wheel to home position i e home hole DN Screw B must be in home sensor and LED corresponding to Screw A home opto sensor goes on Fasten screws Opto wheel End opto c m End hole Home opto a Home hole Check the end position as well move the needle down Adjustment is successful if end LED goes on before moving part reaches end of mechanical range Once this adjustment is necessary never miss sampling needle setting described in the next section 28 Hemolyzer 3 3 1 2 Sampling needle setting This adjustment sets the sampling needle to the operational position In Service menu in Miscellaneous submenu of AJ AJvet and in Service menu of AJB select Needle setting The software moves the needle back and up and turns on horizontal and vertical motors AJ AJvet to keep needle in place AJB holds only the vertical motor during needle setting gt Sampling needle Bottom plane of washing head Teflon stuffing ring lt Tubes to from washing head Check the setting of the needle If end of the needle is at the bottom of the washing head needle is set correctly If not open sc
26. you press LX you will exit from measurement If you press the blank measurement will start Service Manual 69 7 2 2 Database relating warnings 5101 data record s 16 There u Press v selected records present in the database which match the selection 5106 No such type of data There are no Press and check the data records present selection in the database which match the selection 5108 Connect an USB storage This message Connect storage media into the device with data appears at View USB port and press _ external function data record s will be The analyzer will Insert USB storage media and saved on USB save u number of press_W_ storage device data records Insert a storage device into the USB port 7 2 3 Warnings relating to measure Your previous data When you press If you press the new will be lost for accepting values will be accepted and all of Are you sure new QC target the measurements on the values selected QC level will be deleted If you press old values remain 7 2 4 Calibration relating warnings Some or all of the During automatic Check the target values and the calibration factors are out calibration atleast control blood and repeat the one of the factors are calibration If the problem still out of the range 0 8 persists contact the Service or 1 2 Personnel of range 70 Hemolyzer 3
27. zero Name Service Password 729456 This forms a capital A on the keypad 58 Hemolyzer 3 6 SERVICE OPERATION 6 1 Opening the instrument On the right side of the instruments there is a side door which allows reaching of the fluidic system and the mechanical parts Other parts of the analyzer electronic parts etc can be reached by opening the top left cover e How to take off the top left cover First unscrew the 5 cover fixing screws located on the rear panel 3 screws on the upper side and 2 screws on the vertical side After this pull the cover backwards a few centimeters to release sliding locks and then you can turn it upwards to take it off completely Care for internal printer flat cable disconnect it from the printer connector In this way you will be able to reach the electronics 6 2 MDA Monochrome Display Adapter emulation mode MDA emulation mode was developed to help the manufacturer and Service Personnel in the checking phase of the instruments This mode is available during the total operation but especially useful for checking the BIOS setup and OS functions This mode should be handled the same as the service menu the user does not have to know these options At power on the controller PIC display module displays the greeting screen After 6 seconds it starts to scan the keypad and behaves like an MDA adapter to the PC although the displayed screen is still holding the greeting screen If you swi
28. 05 06 07 08 09 a o Pressure 964 7 mBar Set valve On lt UP gt Off lt DOWN gt Toggle lt OK gt 5 2 9 Motor Test Menu N 3 5 gt op 7 49 In the menu on the left you can see the valve numbers and a number under each that represents the actual state of the valve P means peristaltic pump Selected valve number is shown in inverse 0 is Off state 1 is On state Use cursor keys for selecting and setting valve state or press OK to toggle state Pressing numeric key on keyboard the regarding motors start operation repeatedly until pressing 1 or another key All motors stop at initial positions 56 Hemolyzer 3 5 2 10 Display and Keyboard Test Display and keyboard test is provided to check keypad and LCD panel Press a key to test and the LCD will show the code of the pressed key and will be invert the colors at each touch of a key for testing that every LCD dot is functioning 5 2 11 Stress Mode In Stress mode the instrument performs measuring cycles without sample blank measurements continuously This can be used for burn in tests or to check pneumatic system after changing any main fluidic parts You can have information about stability cleanliness HGB operation and counting time stability Results of the last 10 PLT and HGB blank is displayed as well You can detect any kind of noise or bubbles in the system if the PLT is not stab
29. 10 V8 V6 and the remaining cleaning solution is pushed into the RBC chamber with 5 0 ml diluent see diluting process Then the M4 dilutor unit pulls and pushes the diluted cleaner solution via the aperture combined with high voltage burns After this the chambers are drained and rinsed with diluent 4 2 7 Shutdown The fluidic shutdown performs the following steps e Drains chambers e Perform a cleaning cycle e Priming chamber with diluent to avoid drying out of aperture MIX 2 0 ml RBC 5 0 ml WBC 5 0 ml e Sampling needle is positioned above MIX chamber needle up e All of the syringes are positioned down Service Manual 49 5 CHECKING THE PROPER OPERATION There is a built in Self test and Service menu in the analyzer 5 1 Selftest 5 1 1 Self test Screens Self test can be used to check the operation of the instrument Self test ee The first panel shows general information about Model ABACUS 3 as the instrument See menu on the left taps With the new COMB card the PCPNIF firmware Compiled 47 44 2008 version is empty and the MPU firmware version Overall result ERROR holds the COMB version number Overall test result is displayed which can be napak 0 Successful case of every test result is OK or HSB ight er Errors if HIGH LOW or ERROR The panels contain tested parameters as follow ti HGB light dark LED is on off Measuring Electrode voltage curre
30. 11 note that it matches with PAR PDWcv define PAR RDWsd 12 define PAR RDWcv 13 note that it matches with PAR RDW define PAR RDW 13 note that it matches with PAR RDWcv define PAR LYM 14 define PAR MON 15 define PAR GRA 16 define PAR LYMp 17 define PAR MONp 18 define PAR GRAp 19 define PAR RBCt 20 define PAR WBCt 21 define PAR WBCt2 22 Use the above identifiers to interpret entries in the array PARAM and FLAG interpretation should happen accordingly This package starts with the X package identifier contains 2 size descriptor bytes SIZE1 and SIZE2 and the XML file in ASCII format Use this as a descriptor of the structure sent in F package SOH MESSAGE ID gt X lt STX gt lt SIZE1 gt lt SIZE2 gt lt PAYLOAD XML file in ASCII format SIZE1 256 SIZE2 BYTES lt ETX gt lt CHKSUM1 gt lt CHKSUM2 gt lt EOT gt FULL record package This package starts with the F package identifier contains 2 size descriptor bytes SIZE1 and SIZE2 and a record in binary format Interpretation of the binary information should happen using field definitions of the XML file received in an X package lt SOH gt lt MESSAGE_ID gt F lt STX gt lt SIZE1 gt lt SIZE2 gt lt PAYLOAD actual binary record SIZE1 256 SIZE2 BYTES gt lt ETX gt lt CHKSUM1 gt lt CHKSUM2 gt lt EOT gt 88 Hemolyzer 3 10 2 5 Serial Protocol 1 0 Receiver sends that it is ready to receive now lt ENO gt Hemolyzer 3 send
31. 8 2 2 8 2 3 8 2 4 8 2 5 8 2 6 Hemolyzer 3 8 MAINTENANCE Weekly Maintenance by User User should carry out on the first workday before starting up the analyzer Cleaning the washing head User should clean the lower surface of the sampling needle washing head using a soft cloth immersed in warm tap water to remove salt build up Periodic Maintenance by Service The instruments should be checked and maintenance must be carried out in every 6 months or after 10 000 measurement cycles Check Self test and Device statistics Run the built in Self test and check the overall test result Check the device statistics to find common problems Cleaning and Greasing Dilutor Block The dilutor block driving wheels and gear bar should be cleaned from dirt and must be greased with A597 grease between the gear bar and the support and between cogged wheels Checking and Lubricating Dilutor Piston Tips The cogged end of PTFE dilutor pistons should be cleaned and lubricated by neutral silicon grease A599 Apply just a thin layer and move it along the perimeter of the piston so that some of the material goes into the gaps between the sealing rings Repeat this step for Iyse and dilutor pistons as well Check the condition of the micro piston sealing and replace if necessary Cleaning and Lubricating Needle Moving Mechanics The H amp V moving mechanics sliding bars should be cleaned from dust Lubricating of the slidin
32. BC chamber 1 5 Lysing process 1 6 Counting process 1 7 Aperture priming process 1 8 WBC chamber draining process 1 9 chamber draining process 1 10 Cleaner priming process 2 Main functions of the fluidic system 2 1 Initialization 2 2 Wake up 2 3 Measurement cycle 2 4 Standby 2 5 Cleaning 2 6 Hard cleaning 2 7 Shutdown In the detailed process description figures the active tube is filled with black or gray color while an arrow gt shows the direction of the flow Moving mechanic parts have another arrow indicating direction of movement In the section of the basic processes only relevant valves are mentioned in this section ON or OFF while all the other valves are in either ON or OFF state depending on the status of the instrument and other parallel running process Hemolyzer 3 employs a software waste full checking feature Software integrates volume of the reagents used 30 Hemolyzer 3 4 1 Basic processes 4 1 1 Sampling process The aspirating needle aspirates 25 ul 50 ul in prediluted mode of blood sample The Micro dilutor syringe makes the aspirating while the M5 Micro dilutor motor moves down 2 Sway Valve 2 way 3 1 3 Off T Closed Off 2 3 Open There is another sampling process for the second RBC dilution when 35 ul of primary dilution is aspirated from the MIX by the aspirating needle Both of the samples are separated from the diluent with a small air bub
33. C Hemolyzer 3 sends WBC package lt SOH gt DW lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt PID lt HT gt 2 lt LF gt CHN lt HT gt 256 lt LF gt 0 lt HT gt 15 lt HT gt 84 lt HT gt 5 lt HT gt 3 lt ETX gt 69 lt EOT gt Receiver acknowledges WBC transmission and closes the link lt ACK gt lt SPACE gt D It is possible to request for PLT histogram but it is not shown in this example Service Manual 89 10 2 6 Serial Protocol 1 7 Receiver sends that it is ready to receive now lt ENO gt Hemolyzer 3 sends acknowledge lt ACK gt Hemolyzer 3 sends INIT next time to initiate link there is data to send lt SOH gt AI lt STX gt Hemolyzer 3 lt HT gt 2 22 lt HT gt 20011005 lt HT gt 135212 lt ETX gt 30 lt EOT gt Receiver sends acknowledge lt ACK gt lt SPACE gt A Hemolyzer 3 sends DATA package lt SOH gt BD lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt NAME lt HT gt JOE SMI TH lt LF gt MODE lt HT gt 0 lt LF gt WRN lt HT gt 0 lt LF gt PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LF gt WM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 lt LF gt PO1 lt HT gt 6 6 lt HT gt 0 lt LF gt PO2 lt HT gt 4 29 lt HT gt 0 lt LF gt P lt
34. Diskette A 1 44 1 25 MB Primary Master FlashDisk SDTE XX e ADVANCED PNP OS Installed No Reset Configuration Data No gt device configuration Local Bus IDE Adapter Disabled Floppy disk controller Enabled in case of Floppy drive Base address Primary Serial Port A Enabled Base I O address 3F8 Interrupt 1804 Enabled Base I O address 2F8 Interrupt IRQ3 Parallel Port Enabled Mode EPP Base I O address 378 Interrupt IRQ7 gt Watchdog Settings Mode Disabled I O Chip Select Base Disabled Halt on Errors No e SECURITY Fixed disk boot sector Normal Virus check reminder Disabled System backup reminder Disabled e Quick Boot mode Enabled gt Boot Device Priority CD ROM Drive Hard Drive DV XX Slave device FlashDisk SDTE XX Bootable Add in Cards Removable Devices Legacy Floppy Drives Network Boot 60 Hemolyzer 3 6 4 Checking the BIOS setup is suggested to check it if the instrument hangs after switching on or software does not start or cannot be upgraded from floppy disk e Connect an external keyboard US layout to the instrument e Press F2 and keep pressed and switch the instrument e Release F2 when the instrument beeps e Switch the instrument to MDA emulation mode by pressing i on the keypad e Check the key points listed above or from the enclosed Main B
35. Ds Reagent detectors The Reagent Sensor Board is connected to the PPB card and the valve driver micro controller makes the sensing and compensating operations Instrument makes automatic initialization called calibration of reagent sensors during priming phase of fluidics Service Manual 17 2 1 12 LCD Display Module with High Voltage Board LCD amp Keypad LCD module controller Start key connection and status LED LCD backlight lamp Keypad connector Display assembly contains the 320x240 dots color graphical LCD display and the high voltage board LCD has a high voltage backlight lamp high voltage board generates the required voltage There is a special temperature compensation circuit in the display module which makes possible to use the LCD module in wide temperature ranges with the adjusted contrast High Voltage Board HVB generates LCD backlight voltage 300 aperture cleaning voltage 150V and measuring voltage 50V The high voltage board is connected to the system through the amplifier board and the COMB card This unit contains INVC191 inverter which is a high voltage high frequency circuit producing suitable voltage for CCFL cold cathode fluorescent lamp of the LCD The CFSW digital signal from the COMB card controls HVB logical LOW turns inverter on The MVON digital signal from the COMB card switches the measuring voltage 50 V on off by O1 opto switch Warning Be careful with
36. GH Instrument door open Close instrument door LOW HGB head not connected or HGB light HGB LED out of order measurement HGB LED too bright LED resistor on amplifier board LOW Fault on High Voltage Check measuring voltage 50V on High voltage and Amplifier boards LOW Check current generator and test Amplifier test connected to main board coming from the Amplifier Check current generator and test or HIGH Fault on Amplifier board generator FET on Amplifier board Instrument not grounded Check mains ground lead LOW Pressure sensor cable or Check pressure sensor cable Outer pressure or HIGH connector problem connections and controller board pressure connector Peristaltic pump failure Check peristaltic pump HIGH Leakage in pneumatics Check tubing in pneumatics Service Manual 51 5 2 Service 5 2 1 Entering to Service There is a Service menu for servicing and operation checking purposes The entry point is in the Users Service screen where Service Information is displayed Enter code to access Service menu 6484 5 2 2 Main Service Menu The Main Service menu provides access to submenus and service utilities 1 Edit service contact 2 Device information 3 Calibration 4 Settings 5 Testing x B Miscellaneous gt 5 2 3 Edit service contact Here you can edit the Information card fields by cursor keys or by an external keyboard Edit service contact P
37. Hemolyzer A analyticon Servicemanual 1 0 Release Analyticon Biotechnologies AG Am Muehlenberg 10 35104 Lichtenfels Germany info analyticon diagnostics com www analyticon diagnostics com Hemolyzer 3 Service Manual TABLE OF CONTENTS 1 INTRODUCTION 7 1 1 AND SERTAE NUMBER irera rto FW HERE RE EXE can oaedderesvonsaevededuayuabecapsteecdayetstasteey 7 1 2 INTENDED USE TEA VE 7 1 3 INTEGRATED our 8 2 FUNCTIONAL DESCRIPTION e eeeeeeee teen seen tn stats ta sns tn sesta 9 2 1 MAIN ELECTRONIC PARTS OF THE ANALYZER nene enne 9 2 1 1 Counting chambers with electrodes and measuring apertures eene 10 2 1 2 HGB 10 2 4 3 Cell counter Amplifier Board iun ec e tie pet eie 11 2 1 4 Control and Measurement Board COMB with DIMM PC 12 2 1 5 DIMMER C Module 13 2 1 6 Configuration and ID board IDEPROM s sscsscsssesssessessesssessessessessssssesssessesssssessees 13 2 1 7 Pneumatic and Power Board 14 2 1 8 Opto boards for stepper motors eene nemen rem remettre nennen trennen 15 2 1 9 Valve boards ER 15 2 1 10 Pressure Sensor nee ner 16 2 1 11 Digital Reagent Sensor Boddin ee
38. agent containner become empty or the lyse fluid sensor is too sensitive or there are lot of bubbles in the lyse tubes Check the pump and the tubes fittings and valves around the puffer reservoir Empty waste container Replace the Diluent reagent container with a filled one If this error still remains with a filled diluent reagent container calibrate sensors and check the tubings and the fittings Replace the Lyse reagent container with a filled one If this error still remains with a filled lyse reagent container calibrate sensors and check the tubings and the fittings Service Manual 65 Vacuum error The measuring vacu Leakage in the pneumatic system um has been check the pump fittings tubings dramatically pressure sensor puffer reservoir decreasing during and valves the measurement or absolutely lost Pneumatical error Fatal error in If freguently happens this error occurred mechanical check the proper operation of the Device 905 subsystem motor modules home end opto Error code 905 switches ribbon cables and Status moving of motors Retry Check Cleaner container Cleaner reagent Replace the Cleaner reagent container became container with a filled one empty or the clean this error still remains with a fluid sensor is too filled cleaner reagent container sensitive or there are calibrate sensors and check the lot of bubbles in the tubings and the fittings cleaner tubes
39. amber contains 5 0 ml of diluent the wake up process or previous measurement put it into the chamber and the blank HGB measured with this liguid used The RBC chamber contains the last rinsing amount of diluent of the previous measurement cycle or the standby volume The following two flowcharts introduce the entire measurement cycle One of these flowcharts describes the fluidic transfers related the needle the other one the chamber related activities The flowcharts has synchronization points where one of them waiting W for the others signal Needle process Dilutor M3 moves down Sampling primes diluent Needle washnig needle positioning over the MIX chamber Dilutor M3 moves down primes diluent Needle moves down into the MIX chamber 42 Hemolyzer 3 First dilution MIX chamber Dilutior M3 moves down RBC sampling Needle short washing Dilutior M3 Needle positioning moves down over the RBC chamber Second dilution RBC chamber Dilutior M3 moves down Needle up Service Manual Microdilutor syncronization Fill up the MIX chamber with 2 5ml of diluent Fillthe MIX chamber with 2 5ml of diluent Dilutior M3 moves down Perform wake up process 43 44 Chamber process HGB blank delay Measuring HGB blank RBC chamber draining Hemolyzer 3 Dilutor M4 moves down and primes diluent Fill up the MIX chamber with 1
40. and the different NOISE sources are added Sometimes we have to fight one of them but sometimes more Only one of them is enough to make problem 7 3 1 Contaminated reagent The most probable cause real particles are in the reagent and therefore the PLT blank is continuously high e g always 30 40 You can easily sort out this case by replacing diluent by opening a new tank PLT blank must go down is several blank measurements below 10 How can a good reagent become bad by time the reagent tube was contaminated and some bacteria begin to grow inside once you put an infected reagent tube into a new tank by time it can become infected as well i e the background PLT blank becomes high Wash the reagent tube which is in connection with the reagent with 1 of bleach solution then rinse with clean distilled water or diluent It can avoid the bacteria to grow inside If tank is open and cap is not installed or closed external dust can make reagent dirty 7 3 2 Bad earth grounding In this case external ground referenced noise can get into the system by ground coupling If system ground is not good enough ground terminal can become noise source as well i e external signals will be coupled into the system instead of protecting it If no earth ground is available you can use a screw at the rear panel to connect a ground potential to the case so that noise immunity can be increased Measure voltag
41. and to the measuring chamber Sampling needle holder gt Sampling needle XYR opto board Vertical opto wheel Vertical motor Horizontal motor Both stepper motors have optical end switch sensors for detecting these positions These are required for correct initialization and error detection All sensors have status LEDs to show actual conditions The Vertical motor works with a special opto wheel for detecting home amp end positions See the Adjustment section of this manual to place this wheel to the proper position Greasing of the horizontal vertical guiding rods should be done regularly using Photolube A598 a PTFE based thin lubricant It is recommended to check and repeat greasing of guiding rods every year or after 10000 measurements 22 Hemolyzer 3 2 2 4 Main Dilutor There are two stepper motors a common motor opto board four syringes and piston rods with gear transmission Maintenance should be provided to the piston tips by applying neutral silicon grease to the cogged end of the Macro and Lyse pistons between the syringe and the tip itself This will ensure optimum sealing and longer lifetime of piston tips Greasing of the cogged transmission parts cogwheel and cogged bar should be done regularly using machine grease It is recommended to check and repeat greasing of piston tips and transmission gear every year or after 10000 measurements Lyse syringe and piston Diluent syringes
42. ard PPB Wa 2x5 Valve 0 gt gt a PR E E 2x7 2 Ar 5 Internal Printer Main Dilutor Peek F 3a id 4 5V regulator p fixed to chassis PANAT Switch 4 Fuse PCB 7 3 1 2 d c E mi Serial Port E E B PS 2 Keyboard Ne sss eee ee eee ee Liquid Crystal PS 2 Speaker 1 4 2 38 e gt oc al UA x WBC Control amp Measurement Board COMB dmm 5 E 2f I d 2 5 2 10 3 High Voltage Board 3 Amplifier Board 7 777 77 on board CCFL generator e 4 TJ 3 7 LEGEND nesia v ban 2x5 10 pin Double Header Ribbon Cable uie d er x10 1 4 4 pin Single Header Ribbon Cable 3 ve 1x2 options X S shielded 141 Rei power 2 2 wires only 96 Hemolyzer 3 10 4 Hemolyzer 3 tubing schematics Hemolyzer 3 vi v1 vi Vi Tubing schematics Version 1 4 2007 03 14 A Tubing vsd PUFF Pum Mi v2 v3 va 5 Left oj 2 Service Manual 10 5 Recommended kit of tools e standard keyboard PS 2 e Floppy drive with cables A154 J727 J7505 e Screwdrivers Cross Slot Screwdrivers Philips S Slot Screwdrivers
43. artner Your Distributor Press the OK button if a field is completed Address 3 This information will appear in the User Service eS O menu Phone e Contact Conan ERES 5 2 4 Device Information QUT the Device Information menu the model name the serial number the software version and Model HEMOLYZER 3 in compilation date appear Version 050 1363 Compiled 23 04 2009 52 Hemolyzer 3 5 2 5 Service Calibration The analyzer provides a menu for Service calibration purposes In result calculations the service calibration factors are used as the user calibration factors so they are multiplied for each parameter RBCoisp FaCtrsc user FaCtrec serv RBCmeasurea If the user factor is near the bound 0 80 1 20 by setting the corresponding service factor the user factor can be adjusted to 1 00 Example Fact nac user 1 19 and Fact rec serv 0 96 and Fact nac user 1 00 and Fact rec serv 1 14 gives the same result for RBC Apply user calibration factors function is used to combine user and service calibration factors The software will multiply the existing factors and move them to the Service level to set user factors to 1 00 5 2 6 Settings 5 2 6 1 Pneumatic Measurement options Service Person can set the parameters of the automatic functions in this dialog menu Standby time after how many minutes the instrument goes to standby mode default 15 Database capa
44. ble 4728 regulator with cable J7502 34P ribbon cable XY block J7503 10P ribbon cable printer C722 Amplifier shielded cable AP701 Ground cable 15 cm AP702 Ground cable 25 cm AP703 Ground cable 62 cm AP7501 10P ribbon cable reagent sensor AP7502 20 14 ribbon cable LCD AP7503 20P ribbon cable VALVE AP7504 20P ribbon cable HVB board Service Manual CODE NAME AP7505 34P ribbon cable amplifier board AP7506 14P ribbon cable microdilutor AP7507 26P ribbon cable dilutor AP7508 ribbon cable start button AP7509 10P ribbon cable HUR AP132 Foil keyboard AP140 Amplifier board complete AP250 Dilutor block complete AP251 Lyse syringe with piston 202 Lyse syringe S210 Bearing for dilutor J214 Dilutor panel mounted 79 80 Hemolyzer 3 10 APPENDICES 10 1 Warning flags In the followings we summarize the warning flags and give an explanation of their possible cause and a few hints to overcome the problem Uppercase letters refer to WBC or HGB problems Fiag Meaning Recommended user action W three part gt the measurement Possibly Iyse problem warning or WBC Check the discriminators in the WBC histogram If the three part diff discriminators are in the proper place the populations can unsuccessful be separated by eye then the results can be accepted DEE Possibly lyse problem but in som
45. ble and there is another air bubble between the sample and the end of the needle Service Manual 31 4 1 2 Needle washing process The instruments clean the sampling needle with diluent in the washing head after sampling It is important to clean the outer surface of the sampling needle to avoid inaccurate sampling The Macro syringe doses and the pump drains the diluent from the washing head while the sampling needle moves upwards so that the total length of it is washed and cleaned This process is called total sampling needle washing and it is mainly used after taking primary sample from sample tube 374 i NM Y C va 25 GND E VESTES T lt Pump e 4 2 way Valve O ooe DILUENT LYSE CLEANER waste Ux Lewd Detector 23 0 Open Another process which is washing only a smaller of the sampling needle is the same but the needle does not move in the total length Some procedures perform this kind of sampling needle washing The M3 Macro syringe pushes the diluent through V11 Off V12 On The Pump aspirates the diluent from the washing head through V4 On while the M2 Vertical motor moves the sampling needle up 32 Hemolyzer 3 4 1 3 Diluting processes Before the dilutions the MIX and the RBC chambers are fille
46. city mi n Laboratory header size Calendar type Startup dialog Reblank time after how many minutes the 1 Disable multiuser mode instrument makes a blank measurement if it was 3 Reag pack mode in standby mode default 1 hour If it is set to 0 then no ReBlank is performed service settings TA Rinse time If this time is set then the instrument will an automatic extended washing u procedure with more diluent of the chamber s to y B i ig cs time 0 12 hour get wash dirt out of the chambers dust If it is set Waste capacity m to 0 then Rinse is performed Diulent capacity ml Lyse capacity ml 300 Autoclean cycle after how many measurements Bearer capacity 300 the instrument makes autocleaning Reagent pack stability days 9 Disable 3 part diff for using quick lyse without 3 part differential the errors and the bad 3 part parameters can be excluded from the results Disable markers setting this option to Yes will omit the vertical markers from printed reports Service Manual 93 Stretch histogram setting this option to Yes will modify the WBC histogram range from 0 300 fl instead of 0 400 fl this is recommended for Veterinary mode Reagent code This code can be used to modify the offset of the HGB function Enter a value between 0 and 30 default is 9 Extended Probe Voltages This option is ON by default It makes the instrument acc
47. d this makes possible to a request for histograms If no more packages are required lt SPACE gt should be sent MID the identifier of the package that acknowledged by this message If receiving of the message was not successful lt NAK gt should be sent and Hemolyzer 3 will repeat the last package The receiver has approx 1 second to reply Otherwise Hemolyzer 3 will repeat the last transmission twice automatically But if still no response after 3 trials Hemolyzer 3 will not start to communicate any more even if there is data to send see General Description 10 2 4 Detailed Description of Packages The packages sent by Hemolyzer 3 are placed between a header and a tail There are 5 main types of them INIT DATA and histogram packages XML descriptor and FULL record The histogram package can be RBC WBC and PLT Format is the same only the type descriptor differs INIT package Hemolyzer 3 sends the general identifiers by this package during initiation of a communication DEVICE lt HT gt VERSION lt HT gt DATE lt HT gt TIME lt HT gt DB VERSION DEVICE device identifier Hemolyzer 3 VERSION version of the software e g 1 3 DATE date in YYYYMMDD format TIME time in HHMMSS format VERSION Database version in Mmm Major minorminor format 84 Hemolyzer 3 DATA package This package contains sample and patient data the measured parameters and the markers of histograms One data element consists of two
48. d up with 1 ml of diluent This method prevents the chamber from dirt and makes the diluting process faster The sampling process has aspirated 25 of sample or 35ul of primary dilution which is in the sampling needle In the first diluting step the sample is dispensed into the MIX chamber with 3 ml of diluent 1 0 ml is waiting in the MIX chamber which comes from the Macro syringe through V11 On and Micro dilutor while the M3 Dilutor motor moves upwards This process makes the 1 160 first dilution rate in the MIX chamber 1 E x j A _ t T GND zer CPS Pump Stepper Motor UNAL Valve 2 way Valve 1 3 Of Closed Off DILUENT LYSE CLEANER WASTE LDx Detector 2 3 On Open The second sampling process aspirates 35 ul of primary dilution and then it is added with 4 0 ml of diluent 1 0 ml 3 0 ml into the RBC chamber This process makes 1 18285 dilution rate in the RBC chamber Service Manual 33 4 1 4 Fluid transfer from MIX chamber to WBC chamber In Hemolyzer 3 during this step the liguid remaining in the mixing chamber flows via the VO On into the WBC chamber because the vacuum which is in the puffer reservoir and maintenated by the pump aspirates the fluid through V5 On and V3 On while V2 and V1 are Off Simultaneously the peristaltic pump supports this proc
49. e on ground terminal to make sure earth grounding is correct AC voltage lower than 1V is accepted in this case At some places as a bad practice electricians like to connect earth ground terminal to neutral wire Depending on the resistance of the neutral back wire where it is really earthed several volts can appear and this way any inductive noise will be coupled into the instrument It is better to create a real earth grounding and connecting it to the rear screw 72 Hemolyzer 3 7 3 3 External electrical noise If another instrument is near the analyzer can radiate electromagnetic signals in the 1 kHz 100 kHz frequency region it can be picked up by the system especially if they are very close to each other or the grounding is not quite perfect You can easily identify this noise source by relocating the instrument noise high PLT blank disappears In this case you have to identify the possible noise source switch mode power supplies computer monitors since they are not shielded centrifuges due to high switching noise of rotor contacts etc the power of the electromagnetic source because if high power is present maybe relocation does not solve your problems sometimes the electric power supply makes the coupling so UPS solves the problem Another source of coupling in external noise can be the reagent tanks and tubes Especially radio transmitters can cause problems of radiating so that even the reagents diluent guide
50. e pathological samples too high lymphocytes it can be happened or no blank Possibly lyse or diluent problem or duen WBC blank Possibly lyse or diluent problem L WBC RBC limit gt Check the 1 RBC LYM discriminator If it is in the minimum warning point or close to it accept the results Otherwise repeat the measurement If the retried action makes very similar results and the discriminator is in a wrong place then the MID and GRA results are OK but the WBC and LYM results can be higher because of the RBCs Too many RBC cut Repeat the measurement Possibly lyse problem from WBC If the WBC measuring time is too high more than 8 sec it could be aperture clogging In that case perform cleaning and repeat the measurement M WBC coincidence is The results are out of the linearity range Make a dilution too high Linearity with an external dilutor with a pre defined dilution range Do error not forget to correct the results with the defined factor WBC data package Perform cleaning redo measurement aperture clogging Ss errors gt it is a general problem please call your Service Personnel WBC time error The same action as in case of the D warning flag WBC clogging Aperture clogging The same action as in case of the D warning flag Table 9 Summary of warning flags related to WBC HGB Service Manual 81 Warning flags in lowercase r
51. e was run in RBC extended mode not used in code 0x0004 WBCONLY sample was run in WBC only mode RBC related values filled with 0 0 Note 11 Probe voltages max and min values for separate measurements Each value is stored on 1 byte Actual Probe Voltage of given measurement can be calculated as PrVxxxx 50V 1024 Note 12 Age field interpretation Age unit Age gt 128 MONTH YEAR if Age unit MONTH Age of patient 128 this in MONTHS else Age_of_patient Age this in YEARS Note depending on database settings either BirthDate or Age gets filled LimitsLow LimitsHigh and Param match in their structure they contain values for parameters in the order below indices go from 0 to MaxParams 1 WBC RBC HGB HCT MCV MCHC PLT PCT MPV PDWsd PDWcv PDW RDWsd RDWcv RDW LYM MID GRA LYMp MIDp GRAp RBCt WBCt WBCt2 Units can be found on page 4 note 3 Flags has the same order but contains values 0 5 each referring to the corresponding parameter Flags field value interpretation can be found on page 4 note 4 Service Manual 95 10 3 Hemolyzer 3 cabling diagram Reagent Sensor Board Peristaltic Fump e Needie Moving Unit X Y a 2 5 Valve Board 6 12 Pressure Sensor NS 2 5 gt Valve Board 1 5 az gt HN demo e S 1x4 3 J 1x4 3 Micro Dilutor Pneumfatic 87 Power Bo
52. eagents and run priming again 76 Hemolyzer 3 9 SPARE PARTS CODE NAME AP104 Hemolyzer 3 plastic front panel APX104 Front panel Hemolyzer 3 excl keyboard AP132 Foil keyboard A133 HVB board with inverter complete AP136 LCD display A137 Lamp for LCD AP140 Amplifier board Hemolyzer 3 complate J156 Power supply AP157 Mains switch AP730 Loudspeaker J100 PPB board complete AP170 IDEEPROM board J112 Control board COMB 4 12 J113 Control board for DIMMPC A192 DIMMPC CPU A195 DIMMPC safe ring AP300 XY needle movement block complete A301 Timing belt for XY block A302 Stepper motor A303 Bearing for XY block A304 Timing pulley for X mounted A305 Timing pulley for Y mounted A306 Gear for Y opto AP307 H amp V motor opto board complete A308 Opto switch 1 A309 Opto switch II A310 Head washer AP311 Aspirator tip A313 Seeger ring D 19 A314 Seeger ring RA5 A321 Return pulley for XY AP400 Meas Block excl Amplifier Hemolyzer 3 A401 Cone for chambers Service Manual CODE NAME A402 Sealing ring for chamber A403 U shaped metal fixing for apertures A404V2 Cone for WBC chamber A405 Shield for WBC chamber A406 Sealing ring for WBC chamber AP450 MIX chamber AP420 RBC chamber A421 RBC apert
53. edure via V4 Motor INAL Fway Valve 2 way Valve mon De DILUENT Liquid Detector 2 3 Open 34 Hemolyzer 3 4 1 5 Lysing process During this step the primary dilution remaining in the mixing chamber flows into WBC chamber like a simple MIX WBC transfer but in this case simultaneously 0 8 ml of lysing reagent is added through V9 On while the Lyse syringe moves upwards This process makes the 1 192 dilution rate and good mixing with lyse Motor INAL Sway Valve 2 way Valve 0 Stepper Br er Di so Liquid Detector 2 3 Open Service Manual 35 4 1 6 Counting process The regulated vacuum it is generated by the pump in the puffer reservoir aspirates the diluted sample WBC and RBC from the chamber through V2 On valve The instrument counts the cells for 5 seconds in both counting phases at first WBC and after it the Motor t Valve 2 way Valve 41 Stepper e a DE Liquid Detector 2 3 Open 36 Hemolyzer 3 4 1 7 Aperture priming process In this process the aperture branch and backsides of the apertures are filled with diluent and After generating vacuum in the puffer reservoir the pump continues the drainig of the aperture branch V2 and V3 On V1 and V5 meanwhile the diluent syringe of 4 dilutor unit pushes diluent through V10 On V6 On and V8 On 2 Sway Valve 2 way Valve t 3 2 Off T Closed Off 2 3
54. efer to RBC or PLT problems Flag Meaning Recommended user action PLT blank is high Repeat the blank measurement and accept it or no PLT blank Diluent problem Replace diluent open a new tank RBC blank is high Repeat the blank measurement and accept it or no RBC blank Diluent problem Replace diluent open a new tank RBC PLT limit The RBC PLT valley is too high It is rather a diagnostic warning flag If the discriminator is in a wrong place in the PLT or RBC histogram then repeat the measurement for a correct PLT result RBC peak warning Perform cleaning and repeat the measurement clogging If it is a general problem change the RBC aperture m RBC PLT The same action as in case of the M warning flag coincidence is too high Linearity error RBC PLT data The same action as in case of the D warning flag package errors RBC PLT time error gt The same action as in case of the D warning flag RBC PLT clogging The same action as in case of the C warning flag Table 10 Summary of warning flags related to RBC PLT 82 Hemolyzer 3 10 2 Serial Communication Protocol This document describes protocols 1 0 1 7 2 20 2 23 and 3 0 Only protocol version 3 0 contains sending Limits for parameters 10 2 1 General Description Hemolyzer 3 software is able to make serial connection link to a receiver device computer by connecting to the serial port of the instrument If Serial Commu
55. eps e Positioning all mechanical components stepper motors by scanning moving range with end switches Checking the atmospheric pressure Checking of pump and pressure sensor by generating measuring vacuum Priming of reagents and calibrating reagent sensors Fill the tubing with the reagents Cleaning of tubing amp measuring chamber Cleaning of aperture with high pressure back flush cleaner reagent amp high voltage burning 4 2 2 Wake up If the instrument is in standby state the wake up process is taken place in order to make the instrument ready to measure During this process the needle is go out and down to the sampling position and the M1 and M2 stepper motor hold it until the start button pressed or the instrument go into standby While the needle is moving the microdilutor M5 is aspirate some air into the needle this bubble is going to separate the dilent and the blood After this procedure the status LED is turned blue and measurement can be started Simultaneously the MIX and the WBC chamber is drained WBC draining MIX gt WBC WBC draining During the last WBC chamber draining the MIX is filled with 5 0 ml diluent Then this volume is moved into the WBC chamber Service Manual 41 4 2 3 Measurement cycle Before the start of the measurement cycle the instrument is in ready state status LED is green In this state the sampling needle is in the sampling position out and down The Mix chamber is empty the WBC ch
56. ept the physically highest probe voltages to allow operation at lower operating temperature range Maintenance day You can select from week days According to this setting the instrument will ask the user to do weekly maintenance during shut down Laboratory header lines you can choose how many lines to contain the header of the blood result Reagent pack Using packed reagent in Reagent status menu system displays the reagent pack installation day and the Reagent open stability period User can change reagents only together In case of No reagents can be changed separately the date of expiry is ignored Waste container capacity it essential to set the correct volume of the waste container for proper usage of the software waste full alert code 5006 Set this value two liters less than the total volume of waste container Lyse container capacity Enter here volume of Iyse container Cleaner container capacity Enter here volume of cleaner container Rinse container capacity For future development Enter here 0 It is possible but not recommended ignoring the reagent volume check entering 0 as volume of containers Clogging detections bubble volumes for development do not touch them 54 5 2 6 2 Operation Settings Hemolyzer 3 LCD light off delay LCD backlight switches off after 4 hours by pressing key switches back Still the light is off the LED is flashing yellow Disable multi user mode by this sett
57. g bars must be made using A598 Photolube oil containing PTFE Grease or pure lubricating oil is not suitable Checking and Replacing Washing Head Check the state of the washing head and replace if necessary After replacing washing head do not forget to perform correct adjustment of sampling needle height see Section 4 1 2 Checking and Replacing Peristaltic Pump Tube Replace peristaltic pump tube if needed You can check it by opening the lock and removing the tube for inspection Check for leakage of the tubing Reassemble the head Service Manual 8 2 7 8 2 8 75 Warning Be careful DO NOT twist the tube while reposition it the head because it will cause malfunction in a very short period of time Checking condition of lyse tubing Lyse reagent get the tubing material hard at connections air can be aspirated to the system which decreases volume of lyse reagent Check the hardness of tubings at rear panel lyse connection by hand If you feel the Tygon tubing hard compared by other tubing replace them Bleaching of Fluidic System is recommended to run a bleaching procedure to remove stains from the fluidic system Connect 2 5 hand warm clean bleach solution to all reagent inputs and perform priming on all reagent inputs Leave it in the tubing for not more than 2 3 minutes Remove the bleach prime on air Connect distilled water 100 ml and perform priming all reagents again Connect r
58. gt MODE lt HT gt O lt LF gt WRN lt HT gt O lt LF gt PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LFSWM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 lt LF gt PO1 lt HT gt 6 6 lt HT gt 0 lt LF gt PO2 lt HT gt 4 29 lt HT gt 0 lt LF gt PO3 lt HT gt 167 lt HT gt O lt LF gt P21 lt HT gt 8 2 lt HT gt O lt LF gt P22 lt HT gt 5 3 lt HT gt O lt LF gt AGE lt HT gt 24 lt LF gt lt ETX gt 7C lt EOT gt A parameter called AGE is included in the header info This represents the age of the given patient if selected in service menu and defined at sample info screen The interpretation of this value is If the age value is greater than 128 then it represents months in the following way MONTHS AGE 128 months If it is less than 128 then it represents years This parameter has been removed in version 2 23 Service Manual 91 10 2 8 Serial Protocol 3 0 This version utilizes a new approach to record transmitting It sends the INIT package where instrument and PC should establish the communication After connection is established Hemolyzer 3 will send an X package with the XML file to give a description to the binary data sent in the F FULL package From then F packages are sent and receiver software must interpret the binary data using the descriptors in the XML file Str
59. h High Voltage Board LCD and Keyboard controller and Keyboard Panel Internal Printer Pr aum sic and Power Board PPA Ruldie By rium Motor river 1 Opto board contig Mobrcontolk r 2 movlig chambers wit motor Hiv kotoi s he E poss ramur Moroo tole rare r83 opto board HGB meas arg Dpto bando 7 m berdi conkallerid ver 455 86 dre ts Vake bbcks Va E HC fol M Reagestseasor IF E power Boat i rece Se Ho Se N Dinm C AMD Sc 520 Hemolyzer 3 Electronic Functional Block Diagram Am pil tar cell comer J 10 Hemolyzer 3 2 1 1 Counting chambers with electrodes and measuring apertures Impedance method is used for determination of volume and number of cells In this method a known volume of dilution is drawn through a small aperture Constant current is passed through the aperture from one side to the other When a cell passes through the aperture it causes a change in resistance which generates a voltage pulse The amplitude of the voltage pulse is proportional to the ratio of cell volume per aperture volume This is used to determine the volume of cells The number of cells can be obtained by counting the pulses In the instrument there are two Cellcounter Probes WBC probe with 100 u
60. ing the multi user mode can be disabled Instrument startup procedure You can select to start the instrument with Database screen without any pneumatic initialization or with Measurement screen with pneumatic initialization Database capacity You can choose here the capacity of database at installation Changing later all measured data will be lost Using larger database the instrument software startup time can be increased Native keyboard Setting it to Yes will utilize the external PC keyboard with the language specific layout Calendar mode You can select between the Gregorian and the Jalaali Persian calendar All dates will be converted accordingly 5 2 6 3 Edit Service Code Patient data There are two options Birthdate and Age According to this setting both in the sample information dialog and in the database the instrument will prompt for the age or the date of birth If age is specified it can be given in years or months You can change here the default 6484 service code to a maximum 12 character alphanumeric code It is recommended not to change the code if it was changed it is hardly recommended to save the new code Service Manual 5 2 7 Service Testing Menu 55 Service Testing menu provides tools for checking hardware Valve test Motor test Display test 1 2 3 4 Self test 5 Stress test 6 Cleaning 5 cycles 5 2 8 Valve Test Menu Valves 00 01 02 03 04
61. ironment situation as well There are two kinds of HGB measurements e Sample measurement before RBC counting e Diluent measurement in WBC washing phase The HGB result is calculated from these measurements by HGB log CNT gituent tight sample spite of the fact that Hemolyzer 3 is less sensitive to incident light changes it is recommended to keep side door closed during measurements 2 1 3 Cell counter Amplifier Board Amplifier board includes its own voltage regulator the connection interfaces to HGB head to high voltage board and to COMB card In this board there is the current generator circuit which works from 50 V measuring voltage generated by High Voltage Board and the probe voltage DC is amplified with a voltage follower output ELV Nominal measuring current is 870 pA Amplifier board includes two input connectors for the chambers measuring electrodes There are two reed relays on the input side IC10 can select between the two channels RBC WBC with RSW signal IC11 connects high voltage to the selected probe with HSW signal Test circuit makes possible to generate test pulses with TEST and PLS signals through FETs for checking the proper operation of the amplifier channel Connection to the HVB Reed relays Connection to COMB AMP and DIGIO 12 Hemolyzer 3 Amplifier board includes a 3 stage main amplifier channel which gains to the input signal to the 0 5 V range this is the
62. is card The board also contains a 24FC256 serial E PROM which stores the FPGA s configuration data and identity information of the instrument Serial Number OEM model etc EPROM Keeping the hardware identity information write protected IDEPROM allows running the correct software 14 Hemolyzer 3 2 1 7 Pneumatic and Power Board PPB PPB card contains the main power regulator circuits valve and motor driver circuits and other connections for the fluidic and pneumatic system s parts PUMP MDIL X_Y_SR Peristaltic pump Micro dilutor Horizontal Vertical amp connection connection Sample rotor connection PRESSURE Pressure sensor 1 L connection REAGENT_SENSOR Reagent sensor j connection Ymmmr VALVES Valve connections at eae T P 12 IN i Power input gt PRINTER Internal Jem printer connection I PCB CONNID r nnection board connectio 5 voltage regulator Power to Floppy CD DIL_MDIL on chassis Main Dilutor connection PPB card contains the main power regulator circuits valve and motor driver circuits and other connections for the fluidic and pneumatic system s parts Power system generates 5V Digital power 8V Printer power and 12V Motor and valve power from the single 12V DC input signal Motor driver part consists of six separated PIC micro controllers with power drivers Horizontal Vertical
63. le low or HGB has big variation To exit from this mode press the START button at the end of a normal cycle until the Stress operation is finished Service Manual 57 5 2 12 Miscellaneous Settings In this menu you can directly perform Software upgrade this will restart the 1 Software upgrade instrument 2 Clear device statisctics Clear Device Statistics Device statistics number of measurements aperture clogging and other errors can be cleared 3 Clear database 4 Offset adjustment 5 Needle setting 6 Edit users Clear Database The whole Database measurement results including histograms can be cleared Offset adjustment You can adjust the offset on the amplifier board The lower indicator line represents the measured voltage Offset voltage 0 0 mV Needle setting By entering this menu the mv DW 8 needle will to position you can adjust it You can Log in as supervisor if the Multi user mode was selected in the Users Settings submenu Number 4 if present shows the availability of this function In supervisor mode you have the ability change any user passwords and you have full access over user settings Always log out after this supervisor log in 5 2 13 Multi user Rescue Code It the supervisor password has been forgotten there is a rescue code for service purposes to access the supervisor level The Service user data are as follows User ID 0
64. led Analyzer 2 3 1 Hemolyzer 3 Front Panel Built in thermal printer optional 240x128 dots Graphic LCD Function keys Foil keypad START button Rear panel Power switch Warning labels 12V DC inlet USB ports Reagent inlets Serial Port USB slave port PS 2 external keyboard port Service Manual 25 Construction front Valve block Pressure sensor Electronic block amp unit Speaker Valve block Construction right side f Needle moving mechanics Reagent sensor Puffer reservior e Microdilutor Measuring chambers Pump Dilutor block Valve block 26 Hemolyzer 3 Construction left side Pneumatic and Power Board PPB ID Eprom Board IDEPROM Control and Speaker Measurement Board COMB Service Manual 27 3 ADJUSTMENT Mechanical and hardware adjustments are described in this section Software settings are included in Section 5 2 3 1 Mechanical settings There are two important mechanical settings in the system e Opto wheel setting Vertical motor e Sampling needle setting The manufacturer adjusts the analyzer during production However in case of repairs in the mechanical system these adjustments should be checked The omission of these settings can cause malfunction or damages to the instrument 3 1 1 Opto wheel setting This setting is necessary for the vertical motor movements because this adjustment sets the
65. left or right Arrow key Select a different menu up or down Arrow key Move cursor up and down lt gt lt Shift Tap gt lt gt lt End gt Move cursor to bottom of current window lt PgUp gt or lt PgDn gt Move cursor to next or previous page lt F5 gt or lt gt Select the previous value for the current field lt F6 gt or lt gt or lt Space gt Select the next value for the current field lt F9 gt Load default configuration values for this menu lt F10 gt Save and Exit lt Enter gt Execute command or select submenu lt Alt R gt Refresh screen To select an item simply use the arrow key to move the cursor to the field you want Then use the plus and minus keys to select a value for that field The Save Value commands in the Exit Menu save the values currently displayed in all the menus To display a sub menu use the arrow keys to move the cursor to the sub menu you want Then press lt Enter gt A pointer 4 marks all sub menus The Field Help Window The help window on the right side of each menu displays the help text for the currently selected field It is updates as the cursor is moved to each field The General Help Window Pressing lt F1 gt or lt Alt F1 gt on any menu brings up the General Help Window that describes the legend keys and their alternates Press lt Esc gt to exit the General Help Window The Main Menu You
66. m aperture and RBC probe with 80 um aperture Both have a ground electrode assembly and U shaped metal fixing as it is shown in the next figure Assembled Cell counter Probe Measuring tubes _ m rings mark measuring tubes 3 80 Two WBC 100 um d U shaped metal fixing Measuring tube Reference electrode The aperture is made of ruby and it is moulded into the measuring tube 2 1 2 HGB Head Hemoglobin head is placed on the two sides of the WBC chamber It contains light source LED at 540 nm wavelength and Photo Detector TSL235 The Photo Detector converts the light to frequency The HGB concentration is a logarithmic function of this frequency measured by the FPGA circuit of the COMB card Connection to the amplifier LED gt 4 4 TSL235 Service Manual 11 The analyzer performs enhanced Hemoglobin measurement technology for HGB measurement The output of HGB head is freguency TSL235 detector is light to freguency converter This signal is counted by digital counter in the FPGA circuit micro controller This counter counts up while the LED is on and counts down while the LED is off the LED and the counter directions are switched with a 100 Hz signal This method provides real time backlight correction which makes the HGB measurement more precise in changing backlight env
67. ml of diluent Dilutor M4 moves down and primes diluent Fill up the RBC chamber with 1 ml of diluent WBC chamber draining Lysing Service Manual 45 Dilutor M4 moves down and primes diluent Aperture priming Generating measuring vacuum WBC counting RBC counting HGB measurement RBC chamber draining Pull out 0 2 ml of Lyse WBC chamber draining Yes No Fill up the RBC Fill up the RBC chamber with 0 8 ml chamber with 2 5 ml of diluent of diluent 46 Hemolyzer 3 MIX gt WBC Yes No Cleaner Priming chamber Fill up the MIX draining chamber with 2 5 ml of diluent and cleaner Fill up the RBC chamber with diluent Aperture Priming MIX gt WBC High voltage cleaning pulses backflush Dilutor M4 moves down and primes diluent RBC chamber draining chamber Fill up the MIX draining chamber with 2 5 ml of diluent and cleaner Dilutor M4 moves down and primes diluent Fill up the RBC chamber with 4 5 ml of diluent Service Manual 47 MIX gt WBC No Dilutor M4 moves down and primes High voltage cleaning diluent pulses backflush Aperture Priming Dilutor M4 moves down and primes diluent 4 2 4 Standby Standby process is executed when the instrument is in ready state and the standby time see Service Settings ellapsed In the beginning of the standby process the needle goes up and is washed and the chamber
68. n of electronic signal paths In these cases check for any capacitive coupling of electronic signals to the chamber interference with HGB head high freguency signal is coupled to the chamber HGB head metal parts must be grounded The ground comes externally it must be in place otherwise HGB head does not shield but couples in noise interference with internal high voltage inverter high frequency signal is coupled to the chamber Repair is required avoid near contact of HVB cable to chamber or shielded amplifier cable interference with internal start button polling signal to start button may cause noise Guide start button wires as far from chamber as possible You may try mix them up on the start micro switch if applicable interference with display cable high frequency LCD signal is coupled to the chamber by the ribbon cable Keep the ribbon cable far from the chamber interference with CPU fan or other digital logic traces CPU fan or other digital signal radiates to chamber or to the shielded amplifier cable Try keeping the ribbon cables far from the chamber and shielded cable 7 3 4 3 C Bad components or connections bad soldering salt residuals or component failure on amplifier especially if some reagent could get in the amplifier section Cleaning of PCB electrode socket or replacement of amplifier is required Check for the correct soldering of reference cable and its connector circuit board bad soldering or
69. ne een eene ener nene trennen teens 16 2 1 12 LCD Display Module with High Voltage Board esee 17 24 13 Cn 18 2 1 14 External Power Supply esee nn sn 18 2 2 MAIN MECHANIC AND FLUIDIC PARTS OF THE ANALYZER en nene eren enne 19 2 2 1 Sampling needle t t HER re e Re ORE HR OR IS EE 20 2 2 2 Washing hedd u essen IRE MPO EHE Net bI TEE 20 2 2 3 HEV moving unit une ee sn nennen 21 2 2 4 Main 22 2 2 5 Micro Emm 23 2 2 6 23 2 2 7 23 2 3 ASSEMBLBD ANALYZER 24 2 3 1 cte cuoc cux dui Luc dut 24 3 VA LU TOV 27 3 1 MECHANICAESEELTINGS I ORI 27 ILI Opto wheel 27 3 1 2 Sampling needle Setting Em 28 3 2 HARDWARE SETTINGS 28 3 2 1 Amplifier offset setting iter ee rete 28 4 OPERATION OF THE FLUIDIC SYSTEM sorsussonsnssssssnsssssossnnsonsnnsnnsnnssnssnssnnsnnsnnsnnsnnnnnssnssnnsonsnnsnnnne 29 4 1 BASICIPROCESSES 30 4 1 1 30 4 1 2 Needie te 31 4 1 3
70. nication Speed Utilities Settings Customize General Settings Baud Rate setting is set to a valid value other than Offline Hemolyzer 3 will try to initiate a communication sequence Hemolyzer 3 initiates serial communication the receiver must respond to this request The hardware protocol is 8 data bit 1 stop bit no parity If the other side is not responding within 1 second Hemolyzer 3 will repeat the transmission twice but if still no response the other side will be supposed to be not ready to receive data and thus Hemolyzer 3 will not try to communicate any more From this state it can wake up by receiving an lt ENQ gt ASCII code 5 character from the other side and Hemolyzer 3 will immediately respond with an lt ACK gt code 6 From this point Hemolyzer 3 will send data if it is ready for transmission The communication is based on packages There are 7 package types INIT package Device identification software version current date and time DATA package Sample and patient information measured parameters and markers RBC package Sample information RBC histogram WBC package Sample information WBC histogram PLT package Sample information PLT histogram XML file with FULL record structure information FULL record transmission The communication sequence is always started with an INIT package If the link is successful Hemolyzer 3 will send DATA package and the receiver can request RBC
71. nt and m Amplifier Noise test during a 5 second period Nalseitest Oplsi amp sec OK Amplifier transfer by generating 20000 test Amplifier test 19999 pls pulses incl gain related peak value noise Peak 1645 0 OK related deviation on pa gt Atmospheric pressure stands for outer t Vacuum reports pump operation vacuum made vnum nn ag by the pump in a 10 second period of time Drift 0 mBar 1 s ift t B rift represents pressure loss of vacuum Power 12V 421 V measured 10 period time Battery voltage 28 V Power 12 shows the amplifier voltage value Core temperature 38 Power reports voltage value of the battery If battery fails system time will stop Core Temp shows CPU temperature of the Control and Measurement Board COMB At the end of a result line status message is displayed which means that the actual test result is at the normal range OK higher HIGH lower LOW than the pre determined limits or the result is an error ERROR 50 Hemolyzer 3 5 1 2 Normal range of Self Test parameters Parameter Unn Lower bound Upper bouna Heo com o w deviation noise 9 89 News mue 0 8 om moe 0 o Gore Temp TT TT 5 1 3 Troubleshooting Guide for Self test Parameter Mark Possible reason Remegy HGB dark HI
72. oard Manual if you find differences set these settings e Go to the Exit menu upper right quarter of the display and select Exit Saving Changes Confirm this by selecting Yes in the pop up window e instrument will restart with the new correct BIOS setup configurations 6 5 BlOS Description The DIMM PC 520 I is equipped with a JUMPtec Embedded BIOS which is located in a Flash EPROM onboard This device has an 8bit wide access Faster access is provided by the shadow RAM feature default For a detailed description of the BIOS Setup please refer to the section below The Setup Guide With the PhoenixBIOS Setup program it is possible to modify BIOS settings and control the special features of the computer The setup program uses a number of menus for making changes and turning the special features on or off General Information To start the PhoenixBIOS setup utility press lt F2 gt during the string Press lt F2 gt to enter setup is displayed during boot up The Main Menu will be displayed The Menu Bar The Menu Bar at the top of the window lists all the different menus Use the left right arrows to make a selection The Legend Bar Use the keys listed in the legend bar on the bottom to make your selection or exit the current menu The list below describes the legend keys and their alternates Service Manual 61 Key Function lt F1 gt or lt Alt H gt General help window lt gt Exit this menu
73. reagent inputs using cleaning tube kit Remove cleaning tube kit Keep reagent inputs free You can power off the system Apertures are partially clogged Try cleaning 5005 5006 5008 5009 Please waste container empty There are no accepted blank values Measure blank The blank expired results are Measure blank First message of preparing for shipment Second message of preparing for shipment Third message of preparing for shipment The preparing for shipment function is finished During the measurement one or both of the apertures were clogged for WBC for RBC calculated waste volume has reached its maximum level The last blank measurement was unsuccessful The reblank time has expired Remove the reagent tubing and leave waste connected After pressing the analyzer drains itself Connect cleaning tube kit with at least 100 ml distilled water and press After this the analyzer will be rinsed with the distilled water Remove the reagent tubing and leave waste connected After pressing the analyzer drains itself Power off the instrument Try cleaning perform blank measurement If the problem still persists use the hard cleaning function Check and empty the waste container Press If you press you will exit from measurement If you press the blank measurement will start If
74. rews B see above and adjust the needle to the bottom of the washing head Fasten B screws Set the end of the tip to the washing head s bottom plane while the carriage is held by motors Needle setting menu Fix the screws Be careful with the bent upper end of the sampling needle because if badly aligned during movement it can hit other mechanical components causing mechanical jam and therefore damages or error 3 2 Hardware settings 3 2 1 Amplifier offset setting Amplifier offset should be between 5mV Run self test to determine whether offset is within this range If it is out of range it should be re set by the following way 1 Locate the opening for offset setting potentiometer on the measuring block see enclosed picture 2 In Service menu select Offset adjustment menu 3 Adjust the potentiometer to reach 0 mV Opening for offset adjustment on measuring block Service Manual 29 4 OPERATION OF THE FLUIDIC SYSTEM This section describes the steps of Hemolyzer 3 fluidic functions The instruments Fluidic Schematics are shown in section 2 2 of this manual The following figures show total measurement flow diagram and detailed descriptions of basic processes for understanding the fluidic system work The following steps are introduced in this section 1 Basic processes 1 1 Sampling process 1 2 Needle washing process 1 3 Diluting process 1 4 Fluid transfer from MIX chamber to W
75. rinter settings sub menu 1407 Modify the paper setup in the small over the margin Settings Printer settings sub menu Modify the margin setup in the large Settings Printer settings sub menu 1409 margin is too large Modify the margin setup in the large Settings Printer settings sub menu Spacing is too large The distance Modify the vertical spacing setup in 1410 between two results the Settings Printer settings is too high submenu Error d in printer Printer software error Check the connections between the initialization instrument and the printer and 1411 check the printer setup in the Settings Printer settings sub menu Try to make a software upgrade Service Manual 67 7 1 5 Other errors You have to add atleast Multi user problem Follow the instruction or change one user to use multi to single user mode in the User user mode settings submenu s sensor calibration Reagent container Replace the reagent container failed became empty or with a filled one The system turned off the there are lot of If this is still a problem with a s sensor bubbles in reagent filled reagent container turn on tubes the sensor and try recalibrating and check the tubings and the fittings 68 7 2 Warning messages 7 2 1 Pneumatic warnings Hemolyzer 3 Remove reagent tubing at rear reagent inputs Diluent Lyse and Cleaner 5001 5002 5003 5004 Connect min 100 ml distilled water to
76. s the noise A metal pack for the diluent tank then a good earth grounding of this metal box allows this coupling to disappear forever 7 3 4 Internal noise sources The most annoying but real cause is some sort of internal noise The reason for this phenomenon is that inside electrode hot point of the measuring circuit must be well insulated from surrounding electronics otherwise inside noise sources can take their effect 7 3 4 1 Bad chamber insulation shielding of the chamber floating shield couples signals to the chamber and does not prevent against them Check grounding of shield remove it and clean the surface between the shield and the metal base reference electrode connection floating ground reference Repair is required sealing of aperture Replacement of measuring tube is required broken measuring chamber starts to conduct through the gaps ground path Replacement of chamber is required contaminated draining tube starts to conduct due to protein or lipid build up It is very easy to identify this case After replacing the drain tube of the measuring chamber mainly WBC WBC histogram peak or PLT becomes low soon Normally a good cleaner is required to dissolve lipid or protein build up Sometimes the cleaner is not strong enough to keep this tube clean enough Periodic washing using 1 hand warm bleach solution helps Service Manual 73 7 3 4 2 Bad insulatio
77. s acknowledge lt ACK gt Hemolyzer 3 sends INIT to initiate link there is data to send lt SOH gt AI lt STX gt Hemolyzer 3 lt HT gt 1 3 lt HT gt 20011005 lt HT gt 125027 lt ETX gt 30 lt EOT gt Receiver sends acknowledge lt ACK gt lt SPACE gt A Hemolyzer 3 sends DATA package lt SOH gt BD lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt PID lt HT gt 2 lt LF gt NAME lt HT gt JOE SMITH lt LF gt MODE lt HT gt 0 lt LF gt WRN lt HT gt O lt LF gt PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LF gt WM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 lt LF gt PO1 lt HT gt 6 6 lt gt 0 lt 1 gt 02 lt gt 4 29 lt gt 0 lt 1 gt P lt lt gt 167 lt HT gt O lt LF gt P21 HT 8 2 lt HT gt O lt LF gt P22 lt HT gt 5 3 lt HT gt O lt LF gt ETX gt 7C lt EOT gt Receiver acknowledges DATA transmission and requests for RBC package lt ACK gt RB Hemolyzer 3 sends RBC package lt SOH gt CR lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt PID lt HT gt 2 lt LF gt CHN lt HT gt 256 lt LF gt 9 lt HT gt 16 lt HT gt 26 lt HT gt 1 lt HT gt O lt ETX gt F2 lt EOT gt Receiver acknowledges RBC transmission and requests for WBC package lt ACK gt W
78. s are drained After this the 3 0 ml diluent is put into MIX needle and the RBC chamber via V8 and the diluent from the MIX is transferred into the WBC chamber and at last MIX chamber is filled with 1 ml of diluent and the back side of the apertures are primed The diluent in the chambers prevent the apertures and chambers from dirt drying out and salt build up 48 Hemolyzer 3 4 2 5 Cleaning Cleaning function drains all of the chambers and fills the chamber and the MIX chamber with 2 5 ml of diluent After moving content of the MIX chamber into the WBC chamber the aperture branch is primed with cleaner see 4 1 10 The remaining cleaner between the cleaner detector and the V6 valve is pushed into the MIX chamber with diluent After this the WBC chamber is drained and the cleaner with diluent transferred here from the MIX chamber Then the apertures are cleaned with high voltage burn and with backflush The RBC chamber rinsed with 5 0 ml of diluent and the remaining cleaner is pushed out with diluent from the aperture branch and back side off the apertures At least the volumes of the standby state are dosed into the chambers 4 2 6 Hard cleaning Before the hard cleaning a wakeup procedure is executed After pressing the start button the dilutor aspirates 0 2 from the hard cleaning solution Half of this amount is dosed into the MIX chamber diluted with 5 0ml diluent coming from the other dilutor unit M4 through V
79. servicing this board in active state because the high voltage 300V at LCD lamp connector can cause damages or electric shock Start key is a micro switch connected to the COMB card through the Display ribbon cable The status LED indicates the actual status of the analyzer and it has two colors red and blue See User s Manual The LED has three pins and the actual color depends on the controlled pins Start key and status LED are controlled by COMB Connection to COMB and amplifier Connection os d dg to LCD lamp zm un a 18 Hemolyzer 3 2 1 13 Keypad The analyzer has a 29 button foil keypad including numerical keypad 0 9 cursor moving OK and Del buttons and 6 6 function buttons above and under the LCD display as it is shown in the picture below Function buttons Cursor buttons Status LED 2 1 14 External Power Supply The analyzer works with an external power supply The next figure shows the power supply unit generating 12VDC 115V or 230V inlet 12V DC outlet The power supply modules have an auto range input which makes possible to use them with 230V or 115V mains outlet and it has the CE and UL safety certification The input socket of the power supply is a standard 3 terminal plug with power cable connection the output is a special lockable socket as it is shown in the picture Service Manual 19 2 2 Main mechanic and fluidic parts of the Analyzer Hemolyzer
80. sure alias field WBCH value gt Field path Measures Measure field_WBCH alias CHN value 256 gt lt Field path alias Version value 2 22 gt lt Field path Measures Measure alias Version value 2 22 gt lt Field path Measures Measure alias MeasureMode value 3 gt lt Misc gt lt MaxParams value 23 gt lt DB_VESRION value 2 22 gt lt DB_Struct_DAT gt Explanation of a Field of Record tag Record tag should be used to interpret F package lt Starting tag of field descriptor Field name RecNo Name of field can be used as reference in PC application tpe WORD Type descriptor of field length 1 How many bytes should be used for storing length Technical tags gt Closing of tag Field name LimitsLow type float length 23 path Measures Measure alias field_LimitLow incr 1 gt Service Manual 87 This field is an array contains 23 entries as indicated Interpretation of these entries is not listed in the XML file You will find them below define PAR WBC 0 define PAR 1 define PAR 2 always transmitted in 9 1 unit define PAR HCT 3 define 4 define PAR 5 always transmitted in g l unit define PAR MCHC 6 define PAR PLT J define 8 define 9 define PDWsd 10 define PAR PDWcv 11 note that it matches with PAR PDW define PAR PDW
81. t After power on the FPGA holds the DIMM PC in wait state with IOCHRDY signal until the PIC configures the FPGA circuit from the IDEPROM status LED is red during configuration After that the FPGA controls the entire pneumatic system through the Pneumatic bus the Keyboard and Display module with video RAM for MDA Monochrome Display Adapter emulation and Start button amp status LED FPGA circuit also performs measurement data acquisition by using the 10 bit A D chip FPGA makes digital data processing and stores the results in the internal FIFO memory Cell parameters are sent to the DIMM PC by single DMA cycles Service Manual 13 2 1 5 DIMM PC Module The MB4 board incorporates a credit card sized PC named DIMM PC The processor on the DIMM PC a 133MHz Pentium class core with 32Mbytes on board RAM and 32Mbytes on board FlashDisk This is the HDD hard disk drive of the analyzer so instrument software with all user settings calibration database etc is stored on the DIMM PC e DIMMPCO is the Trade Mark of Kontron Embedded Modules GmbH Hard Disk FlashDisk Flash BIOS FlashDisk controller 32 Mbytes RAM Clock generator On board SMPS Super AMD Elan Edge connector SC520 CPU 2 1 6 Configuration and ID E PROM board IDEPROM This board is the interconnection between COMB and PPB cards Pneumatic bus power lines and internal printer signals are connected through th
82. tch to MDA emulation mode the system boot events can be displayed and the CMOS SETUP program can be started if necessary If the main program will not start in 90 seconds the PIC will automatically switch to MDA displaying mode to give information about the reason of hang up This can be some CMOS failure if the motherboard settings are lost for some reason and the system is waiting for user confirmation F1 on external keyboard or running setup both require external keyboard to be connected Hold the i button and press and release the button on the foil keyboard of the analyzer to activate the MDA mode which uses the LCD like a primary computer monitor 80 characters by 25 rows The LCD is smaller than the standard monitor area therefore just about a quarter of the total screen is displayed 40 characters by 16 rows Quarter 1 on LCD Quarter 2 on to change the actual quarter displayed use the arrows cursor keys on the keypad to find the cursor press the i button to go back to the normal graphic displaying mode press i again Quarter 3 on LCD Quarter 4 on LCD Definition of displayed LCD quarters in MDA emulation mode Service Manual 99 6 3 Key BIOS settings for correct operation The required settings are enclosed in this section In MDA mode continuous changing of screen may appear To avoid this press left and up arrow on the keypad in any order MAIN Legacy
83. tings The software version number can be read out from the Device Information or from the Self test submenu Software is absolutely Plug and Play it can read out and detect the type and the serial number of the instrument therefore it will run the correct program for the hardware without any user or service help Every Hemolyzer 3 software version is upgradeable using an USB drive by the latest program developed by Analyticon and it can be downloaded from http www analyticon diagnostics com Service Manual 2 1 2 FUNCTIONAL DESCRIPTION Main electronic parts of the analyzer Hemolyzer 3 contains the following electronic parts 1 10 11 12 13 14 15 16 Counting chambers with electrodes and measuring apertures HGB Measuring Head Cell Counter Amplifier Board behind the chambers CPU Board with DIMM PC and measurement processing unit COMB Board Pneumatic and Power Board PPB with motor controllers valve amp pneumatic controller pump driver and power supply for internal printer 8V and digital circuitry 5V Safe configuration E7PROM board connecting CPU board and PPB Motors with common opto board of needle moving motors H V Main dilutor block with opto board for diluent lyse Micro dilutor block with opto board for sampling Valve boards set of 5 and max 7 Peristaltic Pump Pressure Sensor Digital Reagent Sensor Board Graphic LCD Display Module wit
84. ts own separated opto board located directly in the units Horizontal and Vertical motors have a common Opto board called XYopto Board Connections for Hoirizontal amp Vertical motors Opto switches amp LEDs for Horizontal motor Opto switches amp LEDs for Vertical motor The other side of the board contains a ribbon cable connection to the COMB 2 1 9 Valve boards There are two kinds of valve boards Valve board 0 5 and Valve board 6 12 The valve boards are connected to controller and driver chips are located on the PPB oo Valves Valve Board Connection to 16 Hemolyzer 3 2 1 10 Pressure Sensor This is an MPX5100AP calibrated pressure sensor which can measure the required air pressure and vacuum The Pressure Sensor is connected directly to the PPB card Connection to Puffer reservoir Connection to PPB The pressure sensor can operate from 5V only It is a calibrated sensor with 0 1 1 Bar input range Do not apply more than 1 5 Bar to it because it can ruin the pressure sensor 2 1 11 Digital Reagent Sensor Board This board contains four liquid detector opto detectors optos and a reference opto for automatic temperature and stray light compensation The reference opto is located in the middle and it has the same temperature and backlight conditions as the sensing ones Reference detector Control LE
85. uctures and data for interpreting F record without using an XML parser to get data based on lt Record gt tag of XML file these structures are locked to SW 2 68m typedef struct _DateTime unsigned minute 6 Lf 05 59 64 2 6 6 bits unsigned hour 2 D 0 23 32 2725 5 bits unsigned day 5 1 31 32 225 5 bits unsigned month su If 3 12 16 2 4 4 bits unsigned year 12 0 4095 4096 2 12 12 bits DateTime define MaxParams 23 struct TMeasData WORD RecNo 2 bytes DateTime TimeStamp 4 bytes WORD OperatorID 2 bytes char SampleID 8 8 bytes char PatientID 20 20 bytes char Name 32 32 bytes DateTime BirthDate 4 bytes BYTE Sex 1 byte char Doctor 16 16 bytes float LimitsLow MaxParams 23 4 92 bytes float LimitsHigh MaxParams 23 4 92 bytes char PatTypeName 20 20 bytes WORD VetMode 2 bytes BYTE HistoRBC 256 256 bytes BYTE HistoPLT 256 256 bytes HistoWBC 256 256 bytes PLTlo 1 byte PLThi 1 byte RBClo 1 byte BYTE WBClo 1 byte BYTE LYMhi 1 byte 10 1 byte float Param MaxParams 23 4 92 bytes Flag MaxParams 23 1 23 bytes DWORD Warning 4 bytes float Lyse 4 bytes float 2 4 bytes WORD Options A 2 bytes WORD PrVMinW 2 bytes WORD PrVMaxW 2 bytes WORD PrVMinR
86. ure 80 um A422 Ground electrode for RBC WBC AP430 WBC chamber A431 WBC aperture 100 um A433 Upper part for WBC chamber A434 HGB meas head complete J5P900 Microdilutor unit complete AP501 Valve unit I complete AP502 Valve unit Il complete A504 2 2 valve A505 3 2 valve A506 Valve coil for both valves A507 2 2 valve head A508 3 2 valve head A510 Puffer reservoir Abacus A511 Puffer reservoir holder pair J530 Pressure sensor with cable complete AP240 Reagent sensor board A541 4 2 plastic tube silicon A542 5 3 plastic tube silicon A543 4 1 8 Tygon tube A544 5 3 reagent tube A545 3 2 mm T connector A546 Y connector A547 2 3 3 2 fitting A548 O ring for WBC chamber A549 ring for apertures 77 78 Hemolyzer 3 CODE NAME A550 Diluent cont connector A551 Colour locking ring A552 Colour lock nut inside A553 Colour coding ring outside A554 Luer female A555 Luer male A556 2 3 mm T connector A557 Teflon regent cont Connector J558 Reagent tubing set AP560 Peristaltic pump A561 Tube for peristaltic pump A563 Cassette for pump A564 Fixing for pump pair A565 Connector for pump pair A610 Micro switch APX105 Hemolyzer 3 cover with printer APX107 Complete start bar AP620 Instrument door AP621 Door lock AP640 Hemolyzer 3 sample union A650 Screw for loudspeaker A701 Mains ca
87. ures Measure alias field_WRN gt lt Field name Lyse type float length 1 path Measures Measure alias field_LYSE gt Field name Lyse_2 type float length 1 path Measures Measure alias field_LYSE2 gt lt Field name Options type WORD length 1 path Measures Measure alias field_OPT extID Options gt Field name PrVMinW type WORD length 1 path Measures Measure alias PrVMinW gt Field name PrVMaxW type WORD length 1 path Measures Measure alias PrVMaxW gt Field name PrVMinR type WORD length 1 path Measures Measure alias PrVMinR gt Field name PrVMaxR type WORD length 1 path Measures Measure alias PrVMaxR gt lt Field name PrVMinW2 type WORD length 1 path Measures Measure alias PrVMinW2 gt Field name PrVMaxW2 type WORD length 1 path Measures Measure alias PrVMaxW2 gt lt Field name Age type BYTE length 1 path Measures Measure alias field_AGE gt lt Field name ClogReport type char length 29 path Measures Measure alias ClogReport gt lt Record gt lt Misc gt Field path Measures Measure alias Type value Haematology gt Field path Measures Measure alias field_PLTH value gt lt Field path Measures Measure field_PLTH alias CHN value 256 gt Field path Measures Measure alias field_RBCH value gt Field path Measures Measure field_RBCH alias CHN value 256 gt Field path Measures Mea
88. ve a very little chance to fail only the connections and cables could disconnect which can cause the malfunction of the electronic system The mechanic and fluidic system have a bit more chance to go wrong because it has moving parts This section allows to know what to do when a troubleshooting message appears on the screen 64 Hemolyzer 3 7 1 Error codes s filename concerned d error type concerned error specific string 7 1 1 Software system errors res d on opening file a Fatal system or program error The same as in case of error 1000 1002 Cannot create file s Fatal system or The same as in case of error program error 1000 Data file s is corrupt new will be created all stored measure data will be lost Stored data file is corrupt or missing Disk or software error New stored data file will be created automatically by User confirmation 1004 Error d on indexing file Fatal system or The same as in case of error S program error 1000 7 1 2 Pneumatic errors Fatal pressure error Power off the system Waste is full Empty waste container The system assumes that you will do it before going on Check Diluent container Check Lyse container Cannot make the measuring vacuum Waste container is full Diluent reagent con tainer become empty or one of the diluent fluid sensor is too sensitive or there are a lot of bubbles in the diluent tubes Lyse re
89. well up in the instruments please read this manual carefully to have the knowledge for servicing the instruments perfectly and avoid extra costs and wasting precious time This Hemolyzer 3 Service Manual contains the functional descriptions of the analyzer operation of the fluidic systems adjustments and settings and very important information for the Service Personnel about the service operations and possible problems 1 1 Name and serial number Name Hemolyzer 3 Hematology Analyzer Serial No Every instrument has its own serial number which is printed on the rear panel label and it can be read out from Device Information or from the self test submenu This identity number is write protected by Analyticon 1 2 Intended use Hemolyzer 3 hematology analyzer is a fully automated cell counter for in vitro diagnostic use The compact instrument was developed for small clinics point of cares and vet offices Hemolyzer 3 can process 60 samples per hour and is intended to determine the following 18 hematology parameters from 25 whole blood sample WBC LYM LYM MID GRA three part WBC differential e RDW MCH e PLT MPV PDW 8 Hemolyzer 3 1 3 Integrated software The integrated software controls the instrument operations displays stores recalls data and allows the user to perform QC and calibration procedures and modify the user set

Hemolyzer - Index of

Contents

Download Pdf Manuals

Related Search

Related Contents