LS 6000 Series Scintillation Systems Supplies & Accesories
Contents
1. Solid With highly acidic Non Support or basic samples n H lt 1 gt 1 Aqueous includes Ready Gel or Samples Tissues Ready Value Sample Character istics Org Soluble Tissue Solubilized Samples Emulsion Cocktail Aqueous Soluble Soluble Elute Solubilize Sample GOTO Aqueous Samples Aqueous Samples Cocktail Volume 7 15 mL Standard Vial lt 7mL Mini Vial Ready Organic Ready Protein Page 15 Page 10 Page 11 a 0 12 typical O 0 20 typical sample capacity sample capacity a High constant O High constant counting efficiency counting efficiency Reduced cost for nost commonly sample Packaging Processing nes Bid on Equipment 1 847 683 7720 www bid on equipment com a No two phase breaks ao 0 33 typical sample capacity a No two phase breaks a Count 1 mL sample in mini vials Protein Page 10 11 Sample Volume Cocktail Character istics High Efficiency Gel Cocktail amp Temperature Stability Classic Gel Cocktail Ready Flow lll Ready Value Ready Gel Page 14 Page 13 Page 12 0 12 and 25 50 typical sample capacity 3 0 12 and 25 50 typical sample capacity J Standard gel cocktail at reduced cost OG High efficiency gel cocktail OQ Gel temperature stability 10 30 C Specialized Scintillation Cocktails Ready Protein2. Numbers in parentheses represent volume of sample Other uses of BTS 450 include solubilization of blood serum and feces Chloroform Ready Organic Part No 586600 Description 4 x 3 75 L Plastic Containers 15 Liters For Organic Soluble Samples Ready Organic does not contain emulsifiers and so is not miscible with water This makes it ideal for radioassay procedures in which an aqueous radiolabeled substrate is transformed into an organic radiolabeled product e g enzyme reactions The product may be quantified without prior separation of the reactants simply by adding Ready Organic and counting the radioactivity solubilized into the cocktail phase This is one situation in which two phase counting is advantageous Neumann J R Morency C A and Russian K O A Novel Rapid Assay for Chloramphenicol Acetyltransferase Gene Expression BioTechniques 5 444 447 1987 For Tissues Ready Organic is the cocktail of choice for metabolism and other studies that require quantitation of radiolabeled compounds in tissues It is fully compatible with BTS 450 and other tissue solubilizers as well as other organic solvents used for extraction and precipitation of biological materials High Counting Efficiency With a counting efficiency as high as 62 for 1 mL of some samples Ready Organic yields high counting efficiencies for all samples tested The specially designed fluor system resists the effect of quenching at hi
3. BR 8127A SCINTILLATION SYSTEMS CATAL 3 LS 6000 Series Supplies and Accessories 4 BECKMAN ady Cap Win XteiScint Scfvect Fie Son ofemon Madiun Foe teonvitatie Micro Vokuome Sampies 1 Cutie S Trays of 100 Caps Each Fega No 34181 Packaging Processing Bid on Equipment 1 847 683 7720 BECKMAN www bid on equipment com AMNEREEHITESUIRAMUBINCTETERIE Y LEPAREUIPEIPEHESEUN ENERE HP FP DON VARNES M TAI MARE NV DO OI Print boi eid BECKMAN Herren Act i Ready Cap voe Eui LS 6000 Series Scintillation Systems he LS 6000 Series Scintillation Systems offer the best state of the art electronics available The 32 768 Channel Multichannel Analyzer provides eight times the resolution of other scintillation counters offering A more precise quench monitor with H Number Plus e Superior multilabel studies Quench determination without quench curves Automatic color detection and correction Luminescence correction A unique Programmable Coincidence Gate allows optimization of counting results when using solid scintillators such as Ready Cap and Ready Filter Safer Scintillation Supplies For a cleaner lab and a cleaner environment Beckman offers solid scintillants and an environmentally safe biodegradable cocktail Ready Cap and Ready Filter solid scintillators allow you to count without cocktail for filter samples and nonvolatile samples 200 uL or less An
4. Part No Description 586604 4 x 3 75 L Plastic Containers 15 Liters For Filter Counting Ready Protein contains a patented blend of emulsifiers that is very effective for solubilizing biological precipitates from filters It dissolves nitrocellulose and breaks up glass fiber filters The sample is brought into intimate contact with the cocktail improving efficiency and producing more accurate results It helps reduce or eliminate self absorption quench when counting filters in cocktail Part No 586604 Sample Capacity Ready Protein Although Ready Protein is designed for a 9 sample load Sample Holding Capacity for typical biological samples capacities as high as 30 may Water be achieved with some buffers Refer to the phase diagram Phase Time and Temperature Stability Ready Protein has been tested for single phase stability at both 10 C and 30 C for a minimum of 72 hours At common Single sample volumes i e less than 10 sample load most T samples remain solubilized within this temperature range D PBS Phosphate Buffered Saline te Tris Cl Buffer 1 Quench Resistance Single 4 Ready Protein shows considerable resistance to quench For example when the volume of phosphate buffered saline O 1M NaOH is increased from to 2 mL in 10 mL of cocktail the tritium TAS counting efficiency varies from 46 to 44 6 a drop of Phase only 1 4 xc pn High Counting Efficiency Single As indicated i
5. Scintillation Systems 1 847 683 7720 www bid on equipment com Safer Scintillation Supplies Xtalscint Counting Efficiencies The following values were obtained with dry Ready Caps eee s E LCS ANCHOCO EL DIOEEE CI 05 ee ae If counting in an LS 6000 Series scintillation counter use the Xtalscint option if installed If not installed or when using other counters use a wide open window and turn off the External Standard Quench Monitor Ready Cap Ready Cap is a small container coated on the bottom with Xtalscint solid scintillator It is recommended for liquid samples containing nonvolatile radioactivity that are 200 LL or less Not strongly colored Red yellow green and brown colors absorb the light emitted from Ready Cap In low molarity buffers or organic solvents Less than M is recommended gradient samples of sucrose or CsCl nonvolatile solvents e g glycerol are not recommended Part No Description 534181 Ready Cap Box of 500 How to Use Ready Cap 1 Add Sample Pipette up to 200 uL of aqueous or nonaqueous solution containing nonvolatile radioactivity 2 Dry Use any of the following methods for drying Ready Cap Xtalscint Dryer Uses heated forced air and dries 50 Ready Caps simultaneously in a numbered tray Drying time is 45 minutes or less depending on solvent Heat Lamp Forced Air Convection Oven 60 C Air Dry Ove
6. ea ul A 566777 Dispenser 2 10 mL Includes two 225 mm Teflon filling tubes Min one Teflon discharge tube and one polypropylene adapter and two bottle adapters n 140356 Discharge Tubes set of 2 e i 140357 225 mm Filling Tubes set of 2 Part No 566777 Cocktail Dispenser The 28 mm adapter is for Beckman caps prior to 1987 only For Beckman bottles after 1987 the dispenser fits without any adapter Standards All standards contain scintillation grade fluors and toluene and are permanently flame sealed under nitrogen in low potassium glass standard or miniature vials The calibration of each standard is referenced to the National Institute of Standards and Technology reference material Accuracy of calibration is 3 Quenched Standards to Establish Quench Curves Quenched standards are used to establish quench correction curves Each contains exactly the same amount of radioactivity but is quenched progressively to lower counting efficiencies by the addition of a quenching agent Each set contains ten standards Part No Description Size Calibration standards and quenched standard sets 566681 Quenched C Standards Standard Vial 20 mL 566680 Quenched H Standards Standard Vial 20 mL 566682 Quenched C Standards Mini Vial 7 mL 566683 Quenched H Standards Mini Vial 7 mL Unquenched Standards for Optimal Instrument Performance Unquenched standards are used to establish optimal instrument settings and to moni
7. CDI printer 188031 LS 7000 800 8100 LS 100 LS 3100 P Series Gamma 4000 5500 7000 8000 Built in printers 20 column 2 25 inch Seiko printer 10 rolls 847806 LS 7000 800 8100 LS 100 LS 3100 P Series Gamma 4000 5500 7000 8000 Texas Instruments Silent 733 300 ft roll thermal paper 882749 LS 7000 800 8100 LS 100 LS 3100 P Series Gamma 4000 5500 7000 8000 Texas Instruments Silent 743 100 ft roll thermal paper 838986 LS 3100 T Series 333 345 355 Standard Teletype 300 ft roll Ribbon Part No Description 945637 Epson LX 80 86 ribbon triangular shaped cartridge 929563 Epson RX 80 FX 80 85 LX 800 FX 850 P70RA ribbon long rectangular shaped cartridge as wide as the paper 839986 Teletype 33 ribbon 886287 Teletype 43 ribbon 847591 Seiko ribbon Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com Software quio un EM Hi Hi Tle TE 1 f BP Mii iii i mim i Es aly eei y BRI aha TUE Part No 501016 BSF File Beckman Standard Format ASC File Columnar ASCII Text Format for other third party software WKS File Lotus Spreadsheet Format Usable with Microsoft Excel DBF File dBASE III Database Format Available with Data Capture Support Data Capture Software for Data Acquisition Collect store and spectrum data automatically from Beckman scintillation sample counters onto DOS formatted diskettes The data are save
8. Waer aa Clear 28 Clear pes Bufor 33 Clear 7 ter omna 33 ter 1 ter U1MMOH 34 ew 4 Clear omua es ter Mini vials 1 mL sample 5 mL cocktail Microfuge Tubes 100 uL sample 1 mL cocktail Counting Efficiencies Ready Flow III provides high counting efficiencies even with small sample volumes As the efficiency table indicates counting efficiencies are in excess of 4096 even when counting in Microfuge tubes High Sample Holding Capacity The accompanying phase diagram illustrates that the loading capacity can exceed 30 for a variety of commonly encountered sample types For Use with Flow Detectors On line quantitation and automatic pooling of radiolabeled peaks using Ready Flow III and the dual channel Model 171 Radioisotope Detector from Beckman reduce sample handling and lead to faster experimental conclusions Ready Flow III provides nongelling properties low viscosity and pH stability pH 1 13 Packaging Processing 1 847 683 7720 t Bid on Equipment www bid on equipment com Specialized Scintillation Cocktails Part No 586600 Typical Counting Efficiencies for Tritium Using Ready Organic Percent ficiency in 10 ml Cocktail Sample Volume w 51 dE fu i GN Ne ee ee ee E Sa Se DES eee VENIS Petroleum Ether Diethyl Ether Heptane Ethyl Acetate Methanol Isopropanol BTS 450 Gl Acetic Acid Acetone Methylene CI
9. X Linear d Logl0Y Percent Y 1 Y 8 1 LOG CONCENTRATION 15088 U NEIARIANLOGISTIC STD I U NEIARIANLOGISTIC STF O C NEIARIANLOGISTIC STO O C EIARIA LOGISTIC STU C EITARIA LOGISTIC STL ImmunoFit takes full advantage of the graphical user interface provided by Microsoft Windows m SET UP HIA ANALYSIS LOGISTIC RIA MELE Data file Curve fit Main Menu Total Counts Standard NSB 4 zra i 6 GG G9 GG G9 i Ge Geo Ge e Sample NSB Samples Replicates Dilution Limits ImmunoFit lets you customize assay design easily Requirements Minimum Recommended Multitasking Pentium Memory 4MB 8MB 16MB Monitor EGA V6A VGA HEEL L 5 mu o ow o de Microsoft Windows pp Omm a a a OO OOEEEEAEG Ap GHGLLLG GAGLUL U ILCKtG cLIL a EE a EEE SS SS SS SS Scintillation Systems ImmunoFit EIA RIA 3 0 ImmunoFit performs radioimmunoassay RIA and enzyme immunoassay EIA calculations on data obtained from LS and gamma counting systems and the Biomek 2000 Laboratory Automation Workstation The software is fully compatible with Microsoft Windows 3 0 mouse is recommended Features Pop up windows and pull down menus e Semi automated operation Selection of five different curve fitting routines Correct weighting based on historical error curve Precision profile for assay performance monitoring Automatic or manual Customized printed re
10. bid on equipment com Ready Value Part No Description 586602 4 x 3 75 L Plastic Containers 15 Liters Economical Gel Cocktail Ready Value is the ideal economical choice for laboratories that count large numbers of aqueous samples under standard conditions It eliminates the hazards and mess associated with preparing your own cocktail Efficiency and Holding Capacity Despite its economical price good counting efficiencies can be expected for tritium Additional cost savings can be obtained while retaining acceptable counting efficiencies for many applications by switching to mini vials Ready Value contains no Triton X 100 and so has no unstable uncountable region between 0 5 sample load As illustrated in the accompanying phase diagram Ready Value has a wide sample holding capacity forming either a clear homogeneous solution or a stable gel under conditions of high sample load It can even hold up to its own volume of sample Phase stability for the clear liquid and gel regions has been demonstrated by less than a 396 change in counting efficiency for a minimum of 72 hours over the temperature range 10 C to 30 C This versatility lets you keep one cocktail on hand for a wide variety of samples and volumes Typical Counting Efficiencies for Tritium Using Ready Value Standard Vials Wini vias War s Ger 33 ca PBs Buter 378 ter 319 ce woa ss Cer omnea 359 Ce
11. turn off the External Standard Quench Monitor Ready Filter Applications For detailed listings of Ready Filter applications request BR 7884B Scintillation Supplies and Sample Preparation Guide from your local Beckman representative Note Ready Filter is not recommended for wipe tests Safer Scintillation Supplies Xtalscint Dryer This dryer uses heated forced air to dry up to 50 Ready Caps or 36 Ready Filter circles in three stackable trays Drying time is 45 minutes or less Part No Description 655805 Xtalscint Dryer 120 15 volts 50 60 Hz 450036 Xtalscint Dryer 240 24 volts 50 60 Hz Part No 655805 Ready Cap Drying Trays For use in the Xtalscint Dryer Part No Description 265543 Pack of 10 trays and 3 baffles Part No 265543 Ready Filter Drying Trays For use in the Xtalscint Dryer Part No Description 265544 Pack of 10 trays and 3 baffles Packaging Processing Bid on Equipment 1 847 683 7720 Beckman Instruments Inc www bid on equipment com NENNNs5cc5NNN AMM RM G GV V V V V V V V B Specialized Scintillation Cocktails Selection Chart for Specialized Scintillation Cocktails Beckman also produces a series of cocktails formulated for special applications These are based on pseudocumene a solvent with lower volatility and higher flashpoint than other scintillation solvents like toluene or p xylene Sample
12. 000 Miscellaneous LS 6000 Series Supplies Part No 897516 596281 945638 233006 454928 455140 592883 594716 594945 247971 559981 Description Paper 8 1 2 x 11 inch for Epson printers 1000 fanfolded sheets Ribbon Cartridge for Epson LX 800 or P70RA printer Print Head for Epson LX 800 or P70RA printer Command Cards for Rack Numbers Set of 1 63 and Interrupt Command Cards for User Numbers 1 20 Set of 23 user cards Includes 20 user cards Auto DPM Card Auto Calibrate card and Halt card Command cards for user Numbers 21 50 Rack Standard Vial white Interrupt Rack Standard Vial yellow Halt Rack Standard Vial red Rack Miniature Vial blue Interrupt Rack Miniature Vial yellow Halt Rack Miniature Vial red Bio Vial Racks green set of ten Requires miniature rack system or Versa Rack option Operator s Manual for LS 6000 Series Service Manual for LS 6000 Series Items are supplied with appropriate scintillation counting systems unless not required Scintillation Systems Miscellaneous Supplies for Systems Prior to LS 6000 Series Paper Part No Description 886420 LS 1800 20 column thermal paper 3 rolls 594956 LS 5800 3800 2800 80 column thermal paper 330 fanfolded with perforation HP printer 594957 LS 5800 3800 2800 80 column thermal paper 100 pages per roll HP printer 897261 LS 9800 7800 6800 80 column thermal paper 150 ft roll
13. 300 uL Cartridge package of 1 596807 300 uL Cartridge package of 3 Liquid Cells Part No Description 605335 Liquid Cell pre wound to 200 uL 1 16 in OD 1 32 in ID tubing 605336 Liquid Cell pre wound to 500 uL 1 16 in OD 1 32 in ID tubing 243201 Clear Teflon Tubing 1 16 in OD 1 32 in ID tubing 10 ft 605350 Liquid Cell pre wound to 2750 uL 1 8 in OD 0 093 in ID tubing 605351 Liquid Cell pre wound to 1000 uL 1 8 in OD 0 093 in ID tubing 605341 Clear Teflon Tubing 1 8 in OD 0 093 in ID tubing 32 in Fittings and Tubing Part No Description 605260 Black Drain Tubing For Solid Cells Part No Description Illustration Key 605258 Black Teflon Exit Tubing A 1 16 in OD 5 ft 945670 Red Fingertight B For use with solid cell 960037 Isolation Fitting C For Liquid Cells Part No Description Illustration Key 605338 Black Exit Tubing D For use with exit coil on liquid cell 596809 High Efficiency Mixer E For liquid system 945670 Red Fingertight F For use with solid cell 960037 Isolation Fitting G Supplies Part No Description 247288 Operator s Manual for 171 Radioisotope Detector 929563 Ribbon Cartridge for Epson Printer 897516 Paper for Epson Printer 1000 fanfolded sheets Included with 171 Solid System P N 596800 and P N 596801 Included with 171 Liquid System P N 596802 and P N 596803 24 1 847 683 7720 www bid on equipment com Beckman Instruments Inc Applications
14. 739 ca LIMNaO 38 Clear 33 ce soMUrea 398 Cea 235 Ge Standard vials 1 mL sample 10 mL cocktail Mini vials 1 mL sample 5 mL cocktail Specialized Scintillation Cocktails Part No 586603 Ready Flow Ill Sample Holding Capacity Water 30 C Single Phase Single Phase Single Phase Single Phase Single Phase 20 30 Sample Load 2 3 4 5 6 mL of sample per 10 mL cocktail For Aqueous and Organic Modified Aqueous Effluents Typical Counting Efficiencies for Tritium Using Ready Flow Ill We amp Sampleload 25 Sample Load Sample Efficiency Appearance Efficiency Appearance PBS Butor 33 Gem 31 Clear MEE T WE ciue D m Fawcucv 30 er 37 e sow chene 30 er x3 ter OCHOR 39 Clear 334 Clear CH3CN 80 H20 CH3CN S0 H20 CH30H 80 H20 CH30H S0 H20 14 Ready Flow III Part No 586603 Description 4 x 3 75 L Plastic Containers 15 Liters For Use with Miniature Vials With Ready Flow III count aqueous or nonaqueous samples in as little as 1 mL of cocktail and still obtain excellent counting efficiencies Costs can be reduced by converting from standard 20 mL vials to mini vials or Microfuge tubes also reducing waste disposal costs Typical Counting Efficiencies for Tritium Using Ready Flow III TT inviats Microtuge Tubes
15. 79 888 83 888 48386 668 92 008 228467 83 122 888 127 888 9988 888 134 888 84513 469 411 Fraction Analysis Locate and quantify peaks in isotope spectra or sample data e g molecular sieve and ion exchange chromatography centrifuge gradient analysis Split screen mode lets you display graphs and results simultaneously during Fraction Analysis Features Display of log or linear spectra Display of up to nine files in any color combination Status Use and to change selection 14 22 26 9 Oct 1991 File Management 1 Autosca le Tri Screen 17318 i e Built in library of 37 spectra Single split 2 or tri 3 screen presentations A variety of line styles and data markers e Screen annotation for customized labeling Automatic peak search with a range of options Zoom In Out and Trace functions Addition and subtraction of spectra C NSPECTRANAM 241 BSF C NSPECTRANCL 36 BSF C NSPECTRANBI 287 BSF C NSPECTRANCO 68 BSF C NSPECTRANP 32 BSF C NSPECTRANFE 55 BSF C NSPECTRANCU 64 BSF C NSPECTRANSR 98 BSF e Selection of smoothin g functions Hard copy output to IBM or Epson dot matrix printers Up to nine isotope spectra may be displayed in 1 to 3 separate windows Support of X Y plotter with HP GL interface Part No Description 477076 IsoMAP 1 3 5 inch Diskettes and User s Manual Requirements Minimum Recommended Processor 8088 86 eem or
16. BI ee Data Capture software or data buffer and transfer system is required IsoMAP 1 accepts Beckman Standard Format BSF files for spectrum review from LS 6000 Series counters only For Fraction Analysis BSF files from earlier Beckman counters can also be used Microsoft Windows Packaging Processing Bid on Equipment Scintillation Systems 1 847 683 7720 www bid on equipment com Software Status Use F2 key to edit file name 11 65 11 14 Dec 1991 Preferences Ht Factor Match 163 4 Unknown 163 4 Bkg Iso 1 Iso 2 Iso 3 Iso 4 firea 18944 1008 ISOMAPZNBSFNUNKNOWNS BSF Spectrum with unknown isotopes Status Use and to change selection 11 28 56 14 Dec 1991 Estimate Factors CPM F NISOMAPZNBSFNRESULTS BSF F NISOMAPZNBSFNUNKNOUNS BSF F NISOMAPZNBSFNSS H3 BSF F NISOMAPZWBSFNSS S35 BSF F NISOMAPZWBSFNSS5 P32 BSF Graphical matching techniques are used to identify and quantitate isotope components in unknown spectra Pull down menu with graphics function Redrau Autoscale Save Result Recall Result Estimate Factors Zoon In Zoom Out UnZoom Shade Print Report Preferences Annotate Exit Requirements Minimum Recommended Multitasking Pentium Memory 80K8 4MB 16MB HadDik We ZL Ye e eS p EM wmm o A i T ONCCI dco M Mi rosoft ioen Sao cM LUCI Packaging Processing Bid on Equipme
17. Competition Minimum Recommended Processor 80286 80486 80486 or Pentium Disi tk vex ek Microsoft Windows Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com AccuFit Software for Receptor Analysis AccuFit Saturation Two Site This program computes 1 and 2 site models for LS and gamma counter data from receptor saturation assays AccuFit Competition This program computes 1 and 2 site models for LS and gamma counter data from receptor competition assays Features e Utilize non linear least squares regression analysis to solve the equations that describe the chemical equilibria in the assay tubes No user supplied initial estimates of final answers are required Automated data entry as Beckman Standard Format BSF files from LS and gamma counters Manual data entry is also possible Operate in batch mode up to 91 data files may be processed automatically ideal for multitasking Progressive Complexity Analysis to compute progressively more complex models automatically Built in statistical tests Runs and F tests to aid in unbiased selection of the best computed mode Built in HELP functions Customized printed reports for each assay Hlexibility of experimental design programs use experimentally measured or internally computed nonsaturable binding NSB values Rapid spreadsheet data editing t
18. Option uprades Solid System to Liquid System Menu driven programming guides vou quickly and 605323 System Gold Interface puts the 171 detector on the Gold Bus prog BE TUS y Requires System Gold Software version 3 8 or greater easily through the parameter set up screens with i P easy to understand prompts 605326 pran a Interface Kit for existing 171 Radiochromatography HELP screens explain in detail the input needed to ensure accurate results Two selectable counting windows allow single or dual label counting Pre set Window automatically uses the most accurate counting window even for dual label experiments Simply select the isotope H C P 5S or I Auto Isoset automatically determines and stores the correct window setting for an additional 25 isotopes not mentioned above Packaging Processing Scintillation Systems 1 847 683 7720 ENPE vigens OA Radioisotope Flow Detector Supplies and Replacement Parts Solid Scintillator Cell Part No 596863 Cartridge Part No 596806 Liquid Cell Black Drain Tubing Part No 605260 Packaging Bi Processing for Liquid Cell Bid on Equipment Part No 605351 Solid Scintillator Cells Part No Description 596862 Solid Cell 300 uL includes 1 cartridge 596863 Solid Cell 125 uL includes 1 cartridge Cartridge Part No Description 596804 125 uL Cartridge package of 1 596805 125 uL Cartridge package of 3 596806
19. ample Load 2 3 4 5 6 mL of sample per 10 mL cocktail mL 2H Water in 10 mL pie EM Gel 2mL Efficiency ES 5 30 5 27 0 240 200 CPM per 1000 DPM per mL of Sample 1220 Time in Minutes 8 2 5 2 to Reach 2 Error When counting to a preset time greater sample volume will mean more counts and therefore better statistical counting accuracy TWO PHASE REGION Packaging Processing Bid on Equipment Ready Gel Part No Description 586601 4 x 3 75 L Plastic Containers 15 Liters For Extremes of pH High Salt Concentrations and High Sample Loads Ready Gel is well suited to experiments that generate samples with pH values less than 3 or up to 10 Salt concentrations up to 2 M Sample volume equal to cocktail volume Organic solvents Ready Gel can eliminate the uncertainty associated with cloudy counting conditions Samples are solubilized into a clear solution or in the case of large volumes into a stable gel High Efficiency and Consistent Sample Capacity Stability of clear liquid and gel phases for a variety of common sample types has been confirmed by a less than 3 change in counting efficiencies over a minimum 72 hour period at the temperature range of 10 C to 30 C In practice phase stability for both regions may last for months Holding capacities for these samples are shown in the accompanying phase diagrams Many cocktails have unstable gel regions a
20. and Technical Literature Article Reprints Bulletin No Description 007 186 Liquid Scintillation Analysis of Rn in Water by Alpha Beta Discrimination 007 212 Solid Scintillation Analysis of Rn in Water by Alpha Beta Discrimination Brochures Bulletin No Description BR 7884B Scintillation Supplies and Sample Preparation Guide BR 7885B LS 6500 Advanced Technology Guide BR 7886B PC amp Software Guide BR 7894B Alpha Beta Discrimination on Liquid Scintillation Counters Primer BR 7932B LS 6500 Scintillation System The Counter Solution All trademarks are the property of their respective owners Scintillation Systems Newsletters Bulletin No Description 007 11 Nuclear Counting Exchange Vol No I 007 13 Nuclear Counting Exchange Vol No II 007 122 Nuclear Counting Exchange Vol II No 007 14 Nuclear Counting Exchange Vol Il No Il 007 15 Nuclear Counting Exchange Vol IIl No 007 16 Nuclear Counting Exchange Vol III No II 007 17 Nuclear Counting Exchange Vol IV No I Technical Information Bulletins Bulletin No Description T 1659 Obtaining Radioactivity Data with Model 171 and System Gold T 1715 Calibration of Alpha Beta Discrimination Using the LS 6000 Series Scintillation Counters T 1730 Data Buffer amp Transfer System A Feature to Simplify Data Acquisition on the LS 6000 Series T 1731 IsoMAP 2 A Program to Provide Semi Automated Isotope Identificati
21. bove 25 C Ready Gel maintains gel stability to 30 C Counting efficiency for tritium is virtually unsurpassed by any other high sample content cocktail Typical Counting Efficiencies for Tritium Using Ready Gel StawariVias Mini Vials Waer 48 Cer 2 Ga PBS Butor 407 ew 38 ce Cuwma x dw orma 33 Ger 39 Ga roimNaod 402 Ces 330 Ge Taomurea 398 Cier we ce am Sucrose A Ge s17 Cear Standard vials 1 mL sample 10 mL cocktail Mini vials 1 mL sample 5 mL cocktail Low Activity and Low Level Samples With low activity or low level samples efficiency and throughput can be increased by taking advantage of the high sample holding capacity of Ready Gel For example to count to a 296 2 sigma error accumulate a total of 10 000 counts To do this faster add more sample As illustrated in the table at left although the counting efficiency may decline as more sample is added more CPM are actually measured reaching 10 000 counts more quickly 1 847 683 7720 www bid on equipment com Beckman Instruments Inc Specialized Scintillation Cocktails Part No 586602 Ready Value Sample Holding Capacity Water 30 C Single Phase Single Phase Single Phase Single a Phase qa 20 30 Sample Load 2 3 4 mL of sample per 10 mL cocktail Packaging Processing Bid on Equipment 1 847 683 7720 www
22. d Ready Safe offers a biodegradable liquid cocktail for those general purpose applications when a cocktail is needed A full line of specialized cocktails is also available to meet specialized application needs Tomorrow s Technology Today Flexible Data Handling Software Data handling with the LS 6000 Series is much more flexible because you are not locked into redundant computer technology Applications software packages are available to meet your needs including radioimmunoassays receptor binding studies fraction and spectrum analysis and multiple isotope identification Table of Contents Page Salor Scinilludon SUDBIDS 1 5 pi FER IER DIDUS HU UR RID RUE UD 4 Specialized Scintillation Cocktails sess 9 Cocktail Dispenser and Standards 16 Vials and Miscellaneous Supplies eee 17 DEDE o ARIN TEI sei nies uL DEOS E Re DE i C ERE 18 Radioisotope Flow Detector and Accessories 23 Radioisotope Detector Supplies and Replacement Parts 24 Applications and Technical Literature 25 Use this catalog to order items for your Beckman scintillation system or other liquid scintillation counters For more information on LS 6000 Series Scintillation Systems order BR 7932B from your local Beckman representative www bid on equipmentcom Safer Sc
23. d in compressed Beckman Standard Format BSF files for use with our applications software Features Unique file name for each BSF file saved Each file converts to formats directly compatible with third party software Easy retrieval of your data file while others are still counting Part No Description 501016 LS 6000 Series Data Capture Software For LS 6000SE SC IC TA LL and LS 6500 3 5 inch diskettes User s Manual and RS 232 cable included Data Capture requires a minimum PC configuration of 512K RAM two floppy disk drives serial port and DOS 2 1 or higher Requirements Minimum Recommended Multitasking Processor 8088 86 80486 80486 or Pentium EIN LL NS as CL S ee ww 1 9 M s Microsoft Windows Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com Beckman Instruments Inc Software IsoMAP 1 Spectrum and Status PENNE trreterences Split Seren Fraction Analysis Software IsoMAP 1 uses graphical tools to perform Fraction Analysis Digital Integration of experimental data and Spectrum Review and Analysis of isotope spectra The program operates with or without a mouse 158888 inimum Values Off dic io a m T et ans Spectrum Review and Analysis LM M A NL View manipulate and print plot isotope spectra 41 888 44 888 54446 668 55 888 215533 95 56 888 58 888 85893 328 61 008 165325 88
24. emporary and permanent modes Graphics selection of coordinates automatically scaled and labeled Part No Description 477506 AccuFit Saturation Two Site 3 5 inch Diskettes and User s Manual 471505 AccuFit Competition 3 5 inch Diskettes and User s Manual Data Capture Software is required Beckman Instruments Inc Radioisotope Flow Detector and Accessories CPM or DPM Output corrects CPM results by a constant counting efficiency giving DPM e Normalization Factor per isotope converts results into any unit Auto Background Subtract automatically subtracts the background for every individual run assuring accurate data when using auto injectors Luminescence Correction detects the presence of interfering luminescences prints out the amount and automatically corrects the final result Cell Set self adjusting circuitry for optimal detection efficiency for solid and liquid cells Memory Protect retains all programs during a power outage When power is resumed the 171 comes up Part No 596800 running saving valuable time Cold Room Compatibility ensures stable performance Model 171 Radioisotope Flow Detector in the cold room A dual channel detector designed to accurately and efficiently Auto Start and Stop allows complete hands off operation detect any energy beta or gamma isotope single or dual label when using auto injectors Automatic Fraction Collector control aut
25. gh sample volumes Typical Samples Solubilized by 1 mL BTS 450 Num ee ee MEER Lo eee Pee o Loc Ss eae ae Nerve Cells 50 100 mg 50 140 mg 50 140 mg 80 100 mg Other uses of BTS 450 include solubilization of blood serum and feces Tissue Solubilizer BTS 450 Part No 580691 Description BTS 450 500 mL Bottle Simple Sample Preparation BTS 450 is a quaternary ammonium hydroxide 0 5 N in toluene It rapidly and economically digests proteinaceous tissues and tissue homogenates Up to 0 45 mL of water can be dissolved in 1 mL BTS 450 Just follow a simple series of steps to solubilize and count Weigh sample in a glass vial see table at left Add 1 mL BTS 450 and digest sample at 40 C 50 C until tissue dissolves Decolorize with 30 H 0 Add 70 uL glacial acetic to eliminate chemiluminescence Add 10 mL Ready Organic aRhekaging Processing Bid on Equipment Scintillation Systems 1 847 683 7720 15 www bid on equipment com Cocktail Dispenser and Standards Scintillation Cocktail Dispenser wm This scintillation cocktail dispenser is volume adjustable from Adapter 2 10 mL and comes complete with two 225 mm Teflon filling tubes one Teflon discharge tube and two polypropylene bottle adapters Discharge tubes set of two and filling tubes set of two are available separately as replacement parts o 3 lide la LS 28 mm Adapter Part No Description
26. intillation Supplies Filtration Assays Homogenates Whole Cells TCA Precipitates Polyacrylamide Gels CP etc Course Ground Tissues Ready Filter Page 7 Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com Solid Includes Cell Suspensions Specialized Cocktails amp Tissue Solubilizer Safer Scintillation Supplies Selection Chart Beckman s line of safer scintillation supplies includes our unique solid scintillants for counting without cocktail and a biodegradable environmentally safe cocktail Liquid Aqueous amp Nonaqueous Sample Volume Tissues amp Inert Support s 200 uL 200 uL amp amp Nonvolatile For Multilabel Sample Studies Organs TCA Precipitates TLC Scrapings Polyacrylamide Gels etc Bio Conventional degradable Includes Gel Cocktails Specialized Cocktails Go to Flow Chart for Specialized Cocktails Go to Flow Chart for Specialized Cocktails Page 9 Page 6 Page 5 Page 9 a i M MM a e Ba a TR n Beckman Instruments Inc Safer Scintillation Supplies Ready Safe Part No Description 141349 4 x 3 75 Plastic Containers 15 Liters Multipurpose Biodegradable Cocktail Ready Safe is a liquid scintillation cocktail formulated for use with multilabel volatile samples gt 200 uL It is nonhazardous and has a high holding capacity for reduced cocktai
27. l consumption Part No 141349 Excellent Sample Holding Capacity As shown in the accompanying phase diagram Ready Safe exhibits excellent sample holding characteristics for a wide l variety of common sample types This cocktail may truly be Single n regarded as multipurpose Phase Ready Safe Sample Holding Capacity Water 30 C Typical Counting Efficiencies for Tritium Using Ready Safe StadardVials Mini Vials Waer 458 Cea 393 Clear PBs Bufer 451 e 43 Clear 0MNact 456 tea 45 Clm 11M HG Clear 452 Clear 304 Clear a ae iT Standard vials 1 mL sample 10 mL cocktail Single Mini vials 1 mL sample 5 mL cocktail Single Phase Single Phase 8 0 M Urea 10 Sucrose Phase 20 30 Sample Load 2 mL of sample per 10 mL cocktail Solvent Biodegradability Biodegradation of Cocktail Over Time Where state local and institutional regulations permit Ready Safe is drain disposable helping to reduce disposal 1 costs A comparative study published in the journal Health aon Physics demonstrates that PXE degrades more rapidly than E other solvents used in commercially available environmentally E PXE safe cocktails 8 Data taken from Klein R C and Gershey E L Biodegradable Liquid Scintillation Counting Cocktails Health Physics 59 461 470 1990 Time days Packaging Processing Bid on Equipment
28. m unperforated Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com Ready Filter Ready Filter is a glass fiber filter coated on one side with Xtalscint solid scintillator For ease of orientation the glass fiber side is colored blue Skatron Filter mats are white on both sides Ready Filter has the sample retention characteristics of a Whatman GF B filter This is equivalent to a pore size of 1 micron Part No Description 586310 25 mm Circles 4 Boxes 100 per Box 586302 Mats for Brandel 3 x 16 Harvester Box of 50 586303 Mats for Skatron 6 x 16 Harvester Box of 50 586304 Mats for PHD 2 x 12 Harvester Box of 50 How to Use Ready Filter 1 Add Sample Filter your sample with your manifold or harvester 2 Dry Use any of the following methods for drying Ready Filter Xtalscint Dryer For Ready Filter Circles only Uses heated forced air and dries up to 36 circles simultaneously in stackable trays Drying time is 45 minutes or less depending on solvent Heat Lamp Forced Air Convection Oven 60 C Air Dry Overnight 3 Count Place or punch into glass or plastic counting vials Counting Efficiencies The following values were obtained with dry Ready Filters Counting Efficiency S C Se 70 20 If counting in an LS 6000 Series scintillation counter use the Xtalscint option if installed If not installed or when using other counters use a wide open window and
29. n the table below tritium counting efficiencies of 46 or greater are possible with mL of sample in 10 mL 1 25 Sucrose of cocktail 9 sample load Typical Counting Efficiencies for Tritium Using Ready Protein a a Sample eEficiency Appearance PBS Bufer a mama s www e ner o asm 55 Standard vials 1 mL sample 10 mL cocktail Single l Sample qi 2 3 4 5 6 mL of sample per 10 mL cocktail Packaging Processing Bid on Equipment 1 847 683 7720 Beckman Instruments Inc www bid on equipment com Specialized Scintillation Cocktails Part No 586605 Ready Solv HP Sample Holding Capacity Water 30 C Single Phase Single Phase Tris Cl Buffer Single Phase 0 1M NaOH Single Phase Single Phase Single Phase 3 4 5 mL of sample per 10 mL cocktail Ready Solv HP Part No Description 586605 4 x 3 75 L Plastic Containers 15 Liters For High Performance with Aqueous Samples Ready Solv HP is designed for investigators who need the best counting efficiency with a wide variety of different types of aqueous samples High counting efficiency can increase sample throughput by decreasing counting time when preset error counting is performed High Sample Loads Although Ready Solv HP is ideally suited to the frequently encountered sample size of 1 mL in 10 mL 9 sample load it will hold most aqueous
30. nt 1 847 683 7720 www bid on equipment com IsoMAP 2 Multiple Isotope Identification Software This graphics based program performs isotope identification for up to four unknown isotopes in a single sample and computes absolute activities DPM for each component identified Valuable for environmental scientists and health physicists performing radioactive waste disposal monitoring Features Direct data entry of logarithmic BSF files from LS 6000 Series scintillation counters Graphical comparison and matching of an unknown spectrum with up to four isotope library spectra Display of spectra in any color combination Automatic channel by channel subtraction of a background spectrum from the unknown spectrum Automatic estimation of factors for spectral matching On screen display of keV and channel values Automatic DPM computation for each identified component in the unknown spectrum e Screen annotation for customized labeling Zoom In Out for detailed spectral matching Hard copy output to IBM or Epson dot matrix printers Part No 477077 Description ISoMAP 2 3 5 inch Diskettes and User s Manual Data Capture software or data buffer and transfer system is required IsoMAP 2 accepts Beckman Standard Format BSF log spectrum files from LS 6000 Series scintillation counters Beckman Instruments Inc Software ImmunoFit EIAJRIA Graphics 358 Linear X Logi X v Log e
31. omatically controls peak collection detecting the beginning and 171 Solid System 115V 50 60 Hz end of each peak and pooling it into one tube 596801 171 Solid System 240V 50 60 Hz 596802 171 Liquid System 115V 50 60 Hz Dual Analog Output provides single or dual analog 596803 171 Liquid System 240V 50 60 Hz tracings on tne chart recorder Solid System includes Quick Change Cell two interchangeable cartridges Radioisotope Flow Detector Accessories 125 uL and 300 uL Liquid System includes 110B Deluxe LC Pump Flow Rate Controller P oe lene iqui ystem Wictudes etuxe ump ow Kate Controller F unm eU Stand High Efficiency Mixer and Liquid F low Cell of variable volume 200 uL 523903 Scintillation Test Kit to 2750 uL 596852 UV Tracing Accessory receives analog output from a second detector UV electrochemical etc Requires printer P N 523096 and Peak Plot Software P N 596855 or recorder or computer Features 605313 High Resolution Analog Output Accessory is recommended w n f tput t xt int t Liquid Crystal Display provides 4 line 40 character 2 E z ENN P oon 4 s ki icd pe 596830 ream Splitter is used with the liquid system to fraction detailed easy to read prompts off any samples prior to mixing with scintillant Eight user programs can be stored ready for use at the 605316 Manual Sample Holder holds one 12 x 75 mm test tube press of a button 596851 Liquid System
32. on and Quantitation T 1760 Radioactive Waste Manager A Convenient Tool for Automatic Radioactive Waste Tracking on LS 6000 Series Scintillation Counters Available with BR 7885B LS 6500 Advanced Technology Guide Available with BR 7886B PC amp Software Guide Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com
33. ports for each assay Built in context sensitive HELP Part No 586406 Description ImmunoFit EIA RIA 3 0 3 5 inch Diskettes and User s Manual Data Capture software or data buffer and transfer system is required Packaging Processing Bid on Equipment 1 847 683 7720 www bid on equipment com Software SUMMARY TABLE Computed Parameters PR1 FILE SAMPLE PR1 COMPETITION CM1 FILE SAMPLE CM1 DTi FILE S MPLE DT1 SM1 FILE SAMPLE SM1 WEIGHT UNITY Protein Conc mg m1l1 2 5 Assay ID Unnamed Model 3 gt lt gt LINEAR lt Kdi Mo c a 1 000E 08 IC50 2 031E 05 Kd2 M lt c gt 1 000E 08 Ki 9 020E 06 Kd3 M 1 043E 05 3 1 815E 06 Hill 93E 00 Kd4 M j 2 929E 05 Kic 8 953E 06 Bmaxi M prep 2 203E 10 5 1 191E 10 Pts 9 pt 1 226E 10 3 953E 03 gt 1 172bE 06 1 066E 06 7 617E 07 5 629E 12 5 875E 12 1 689E 04 gt 4 643E 22 3 392E 22 7 931E 23 7 913E 23 Progressively more complex models are computed automatically and the results presented in the summary table DATA FILE DEMOO1 MODEL 4 WEIGHTING UNITY BOUND FMOL MG PROTEIN 0 25 FREE NANOMOLAR 1 Data may be displayed and printed in a variety of graphs Requirements Saturation Minimum Recommended Multitasking Pentium Memory 8 amp AKB 4MB 16MB dad ol E a ee C LM 50 5 NES oM o o o Windows Requirements
34. rnight 3 Count Place in standard 20 mL glass or plastic counting vials The Ready Caps are easy to remove and the vials may be reused without fear of contamination Ready Cap Applications Whole cell and membrane receptor assays for counting reference dose standards transport studies enzyme assays radioimmunoassays thymidine incorporation tumor response assays counting of alpha emitters with low background cytotoxicity assays counting whole blood without bleaching For details of use of Ready Cap with these and other applications see the Scintillation Supplies and Sample Preparation Guide BR 7884B Beckman Instruments Inc Safer Scintillation Supplies Part No 586310 25 mm Filter Circles Filter Area Depends on manifold Part No 586302 Filter Mat for Brandel Harvester MB 48R Filter Area 250 mm perforated a Pa Ae M 0M ae PS uf Td NV N MN ou T un My JN S A Mu i wo a Y Y WWW Ce Nas BE NL SH N AVI nt ln ln lie a T K i x c 4X N33 3 A as S ur vul Su S n li t M 1 4 AN et et o m Hi X oW S 4 tus Nas Nu 4 T 25 7 cm Part No 586303 Filter Mat for Skatron and Compatible Harvesters Filter Area 112 mm perforated oooooooooooo0 6 GoOoouuooooOoo o y 3243 66 Part No 586304 Filter Mat for PHD Harvester 200A and 290 Filter Area 20 m
35. samples as a continuous clear liquid emulsion from zero to the recommended capacity with no two phase breaks High sample load means that you can add more sample or use less cocktail Using less cocktail keeps operating costs down Time and Temperature Stability Each lot of Ready Solv HP is tested to ensure single phase stability at 10 C and 30 C Samples incorporated into the cocktail are stable for counting for a minimum of 72 hours at these temperatures This means that whether you count your samples overnight or on weekends you can be assured of stability of counting conditions irrespective of temperature fluctuations Use the accompanying phase diagram to determine sample load percentages that are free of temperature effect Constant Efficiency Ready Solv HP cocktail resists the quenching effect of increasing sample load For example while tritium efficiency for a 1 sample load is typically 45 it is still at 42 if the sample load is increased to 16 This means that counting efficiency remains relatively constant with a variety of aqueous sample volumes so that quench correction may be unnecessary Packaging Processing Bid on Equipment wr 1 847 683 7720 11 www bid on equipment com Specialized Scintillation Cocktails Part No 58660 Ready Gel Sample Holding Capacity Water oc Single Phase Single Phase Single Phase Single Phase Single Phase 20 30 S
36. tor long term instrument performance A set of three standards is provided C H and a background standard Part No Description Size 566321 Unquenched LS Standards Standard Vial 20 mL 594946 Unquenched LS Standards Mini Vial 7 mL One set of unquenched standards is supplied with LS 6000 Series scintillation counting systems Packaging Processing Bid on Equipment 1 847 683 7720 Beckman Instruments Inc www bid on equipment com Ja Fee ee d aa VP a Vials and Miscellaneous Supplies 26 9 mm 18 3 mm 14mm T 27 3 mm 17 mm 14 1 mm Mini POLY Q Vial POLY Q Vial Bio Vial 18 mL 6 mL 4 mL Plastic Vials POLY Q and Mini POLY Q vials are made of high density linear polyethylene and have a very low permeability for the organic solvents used in LS cocktails The Bio Vial is manufactured from high density polypropylene making it translucent and unbreakable ideal for low speed centrifugation procedures These vials reduce sample handling time as the vial caps either snap on Bio Vial or twist on POLY Q and Mini POLY Q in a single movement Tight and leakproof the caps are smooth and have no serrations to irritate the hands when many samples are being capped Description Quantity POLY Q Vial Polyethylene 18 mL Tray Pack of 500 POLY Q Vial Polyethylene 18 mL Bulk Pack of 1000 Mini POLY Q Vial Polyethylene 6 mL Bulk Pack of 2000 Bio Vial Polypropylene 4 mL Bulk Pack of 1
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