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SERVICE MANUAL - Frank`s Hospital Workshop

Contents

1. I zaif B uq M HESATIIM E O11 15 7239 T AU 0 aie 15445 Fle der 3972 Www SNOISNINIG 0 15 3SUMM3HIO 30 50 900 S310H UO amp XXX Zoek 206 MOL NRO 3 NJA SLOSHZ SINO Nid H 3001 td tar 3401 Lo T 1051403 3a ic 4 VALOJN ct 400 DOWN 1 LOI 900 oNIBI3HIONS 07 3903 gavus D 0771 Oi w i ne Ec 1 22 OL MYOM 59303 3AON3H d 13709 ul di LI 45 94 9 a o l _ _ hated 3194 82 1 39 m ux D Rd uma me GAP RS ue ST QN M T zt DP eiectus cce i a S AGTL OCL Dt Drago F wd i P Jow JS LU 147 3l f diWid FOHZYNOTT3N 250 2222 HBGIION 123 12365 1 QD 158 2330 40 S 3 I J R DL d c9 n i 2279295 031915 35 e3HIO 523190 930 C c
2. NOISN3DG VINIS SEE M 65 SUNNA 1 011 AOOE 30 600 ONY SUNN 092 022 ADDL 37 0600 5 125 1NVIHVA SPY ONILd3OX3 Wild NOGYVO AGSZ O SMHOAL3N NI JON SHOLSIS3M TW 310N 19 Wey i iZ O INI 128 08 Ae g 9N N A33 AVEOVIG ONIMIM HSHSVM HSVMTUM 961 30 DESNO 3HL 30V 38 JON ISNA M 53409 3 vOSL v00 IBNDN ONIMVHO pact eere 25506 CRINE 1 yn SO BIND 609 STH 9 XX FOF XX N35 7 N33489S MOTI3A N3339 Q31V1S 3SIAWM3HIO SSTINR 20 91 TW 0 2 020 NOISH3A LNWINWA A339 GBINLS 3SIMH3HIO SSTINN 270 91 TIY AOPZ OZZ 5 2 INVIMVA 031915 35 SS3Nn 270 91 ANIM
3. 10 11 Prime IndiGator eire cer Dan m exa dir tu Supa 11 IE MEM P HM 24 26 Prime p sh DUMON 11 ERR RI 24 28 teserVoll eps ncaa eds Savane ee cade eee 10 Programi 10 30 PLU UU tis ede cen be rey 10 WASta ATION eacus e 18 Q Quick Reference Guidea ie re a e e e b e a a d re E Po testes 63 Reagent DOTS EE 10 37 Reset DuttOh nu ideas 11 27 28 34 Rotary swItch85 tre pp odes CA th cd taba p eti 32 Rowselectibtt oli S EPA 11 Row selectndicators 11 countdown sequence ces inae actus dn pares 23 26 S Safety Syrmibols u S P E etre 7 Selecting progra Mirota gaa tea ma Pear ir pa a ven 30 SEVICE RP MEI 51 SIUtCOWE 3 o rer v ea dr d t ETE 34 Cais 4 PT 34 Site OF asss 15
4. esos m i Fun 198 pd e 38nL WoNj 38 OL OL G3lili SYIMYYN YN0109 LON 311108 3SN3dSIQ S 1Y 100 777 715 ANANDA
5. A 11 27 28 49 15 TEST IIT TENERETUR 33 Warning signal eie eie DEO RIBERA E 30 Warranty 12 16 57 Washlhrarnm assembly tete E RE eu 10 Washi EID 10 WMS tall At ON s Su 21 Wash head 22 holder azu 17 installatiODrz 3 oa beni a eR ea 18 23 UNClOGGING 42 Wash mode Ena DES 31 Wash reagent MP 37 DO oett clt T ss 41 49 unequal dispensing or 49 EFIE kasa 10 Waste S m OSO A ak 10 37 overfilling precautions l irren nnne tke 19 14 172 13 15 54 Working areas 15 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 62 Chapter 13 Glossary and Abbreviations 13 Glossary Abbreviation
6. 16 Thiings to avold REGERE ERA 16 Technical prerequisites i tenti rr Er 16 Installation a uray 17 How to install the pump unit and washer unit connections 19 Wash head height 5 iren tir 23 Mains supply cable nune iW 24 Changing wash heads 24 Operatioral check Fra naa a A 24 lI pue 26 Operational CheckliSts oi roii Fa mri nea PG De EX TAE 26 Washing 27 Priming DrocedUre i agua bias ie ene eb Pee ete rhe 29 Adding wastlireagernt e ere REESE 30 Program Sel CH OM eunte ib reete Seems 31 Two way switch functions nnne nennen nnns 32 Rotary switch 33 pato de pide 35 Sliutdown procedure 35 Daily shutdown 35 Long period shutdown eere e gne 35 Emergency 5 5 gni ead ee 35 u DS Su 36 Regular and preventive maintenance sess enne 36 I 36 Maintenance 1 444000000 36 se tite
7. 54 Feedback FOM Es 65 Frequency 10 16 FUS691 54 43 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Chapter 12 Index H HOSE CONMECLOMS uiii ici ankaqa ceu da 20 How to disinfect the instrument nasa 34 57 eiie red ve icd ve beret em vet e Eie den Mata ber ra den 24 30 INGICATONS ede ade cc 11 ce 12 e BETE A E 2 Ete 12 E 15 Installing liquid level 19 pump and washer units 1 242 4 400 040 2 1 1 00 u 20 Instrument decontarrnltiatiori gt du es 45 10 INS PECTING Ea kauki a ond rcd
8. nad Unpinching tlie Tube sema e gre at ted e bua en La aea RR ee ena 42 De clogging the wash head oi i ioi e ea e d dia 43 Opening the fuse holder of the washer 2 44 Washer unit fuse and fuse 22 99 e e ea a a a a 44 Opening the fuse holder of the pump unit 45 Pump unit fuse holder opened 45 Jristallatio Chee E tts e a d no e 13 Silicon tubing color codes Aug Operational checklist irii e ee A u eR 26 Number of washes after a soak period 33 Aspiration after the final wash ui pernod NTNUS s i eee e d v e 33 Pause seconds between each washing 34 Number of washes before soaking 2 34 Washing volume ul 424 Maintenance check list ESTO Troubleshooting lists ea i i o i oed dai 50 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Chapter 1 Safety Symbols Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated Safety symbols and markings used on the Wellwash 4 Mk 2 Power ON C Power OFF SN Serial number REF Catalog number Date of manufacture Consult Instructions for Use In Vitro Diagnostic Medical Device WEEE symbol This p
9. NINO INVIBYA SNIBRL 15 01 434 did HUA H3ON31IS SN SOd 2 Sluvd ONIAOM JO 5319 SIHA HILHAS 3HDSN3 3 43H Manu Lo Du wonmis AGNOS Ol AN3OV TOV 3015 50 700 POPs z SOHNVGNYIS DHIIISHIGNS INGHHDO Hem NLEY Sussex 14 9EY England Item Drawing No m Description 1 004 101 CHASSIS PUMP UNIT 1 2 3 4 004 067a PUMP SUB ASSEMBLY 1 5 004 330 MODIFIED ELBOW CONNECTOR 1 6 000709004 SOCKET FUSE HOLDER 1 7 000412396 FUSE 1A A S 2 8 9 000709008 MAINS SOCKET 1 10 11 50 019 SILICONE TUBE 630 12 004 062 VACUUM TUBE ASSEMBLY 1 13 000757017 ELBOW CONNECTOR 1 14 000706038 ON OFF SWITCH 1 15 000727005 PIGGY BACK CRIMP CONNECTOR 2 16 000727002 SHROUDED RECEPTICAL 3 16 6 17 18 000743017 FRICTION BUSH 2 19 000780007 M4 EARTH TAG 2 S PROOF 20 000891001 EARTH SYMBOL 1 21 22 000827003 SLEEVING H20 x 20 8 23 C2266 WIRE 16 0 2 EQUIP GREY BLUE 310 mm 24 C3259 WIRE 16 0 2 EQUIP GRN YLLW 305 mm 25 C1563 WIRE 16 0 2 EQUIP BLUE 80 mm 26 C1561 WIRE 16 0 2 EQUIP BROWN 90 mm 27 C6060 WIRE 16 0 2 EQUIP RED BRO
10. 1514319 Document Release Page 004 006 100 X 3 0 9 ThermoFisher SCIENTIFIC WELLWASH 4 2 SERVICE MANUAL Miscellaneous Spare parts ITEM CAT D T Em 1210520 Mains cord B RO Ree 1210550 Fuse 0 5A 220 2 unu ees a u ee 1210840 F se 220 24 OV ette tenete e E ERE 1210890 Fuse 2 0 110 120 2 0 0442000 1210920 0 7 10 120 2845 PH ED 000716001 Silicone tube 3 18 x 1 98 een ener nennen entis 000788004 Silicone tube 50 15 000788005 Silicone TUBE 5 5 X 2 9 ciue ORG Dr 50 019 Pump Service 000837001 Declogging IDOL Laune eoo ER tape 004 319 Packing materials Wellwash 4 AMK2 eee 1514417 Service manual Wellwash 4 AMK2 WW 004 853 Preventative maintenance kit for 4 2 5 00099 Document Release Page 004 006 100 X 3 0 10 ThermoFisher SCIENTIFIC WELLWASH 4 2 SERVICE MANUAL Wash Hedd sesope gissingen iiaiai WW004 1 12 way Programme Card 1 and 4 washes
11. ol leeds 64 ej re dri M 12 35 COG GING S 42 Preventi oasis aan 34 Color coding of the tubes 18 5255 EE 1 Consumables warranty limitations RR 57 Contamination SPECIMEN ASSN 27 Control 11 Countdown sequence of row indicators 4 404 44 1 4 nnn nnns 23 2 25 2 45 Certifiealerof uuu 64 a u catia ands dod co aaa s aN apasi 45 DIMENSIONS uum u s Ra LEE 54 55 45 Disinfection procedure 34 DISPENSE 33 2 Tx alay aa UNTER RR 54 Disposal RETE TIE DIUI RR 47 DET cm 45 Environmental 1 u
12. 1430 1 Hn 45 ISN SWill 335 1 5 C s IBO FO E 900 F STOH 10 5 xXx NOISH3A NONVONO 2 MR 31310580 von G3AN3S34 p cz 06 200 NOISH3A AAC A A 31310580 9 NOISM3A 0 _ SINVIMVA 1 SONIS 19300 OL 260 OL 8 Will 9 NNO OL 5 031 303 8 WALI NI S HOH SL INO Y 8 RW FE GL vOO 335 8 INVIBVA 264 700 33S 3 3 O V SINVIMVA ul 553034 NI SZ LL WIU Z 9661 4dV 6 gt j ONIN3IHOLL 3Sv3N9 3NOOHIS SNIJYNS ANY N31HOIH3AO LON OQ SONIXIJ 1544 S NI MIND N3IHSIL NN201 OL AJANG ONISN SINN N3IHSIL H3JAO2 Ol 380438 WWZ 1 30 3SIWH19N31 MOB 3AYONOO V SVH L 3UnSN3 l 310N 9 SALON 336 335 09 6 P 8 7 3 2 Sl AGU HUM Z 30 53903 Tyas 338 fear
13. HOUMS HOJ ANO 4 INVINWA Z ISd S 9 OL 9 OL 3H SS3Hd 135 1 S310N per om 966 81 SOL ZHOS 021 011 NOISH3A a ZHOS O Z OZZ NOISH3A TALON NMOHS Y3A09 3 NOLLO3S NO MJA 18494 SN SOd 2 gt E 335 BION 335 INGALYVd30 SNDDVd Ol H3JSNVHL OL 1531 5343 9 Lid 3 05 LISNVMI AINO 340236 335 52 HGOND ATNIKL Ov3HdS 38 01 SZ Wall V V V NOILO3S 081 wa usep 004 050 004 054A CHASSIS ASSEMBLY PUMP UNIT 004 636 CATCH BOTTLE CAVITY LID 50 019 SILICONE TUBE 1465 LONG INN 4 BOTTLE s UTILE NEMPE 1 2 2 000804010 WASHER 4 PLAIN 1 9 APR 1996 w i 2 gt r X B Mmm mane a a aaacapuwqywanaqananamapayvayamaraa Q 4 o B pa pa o y b h we N NIN 4
14. BILLINGSHURST FADENLEY pee eus j L APPROVED THIS DRAWING IS THE COPYRIGHT MC NEM DR ORE S DOWNES DENLEY INST LTD COPIES bog al WHO T NOT wc x WITHOUT THE WRITTEN CONSENT oF 20 259 88 137 pare ox ox 145 1984 TITLE SHEET DEPT I LIST FOR DRG No DRAWN d GENERAL ASSEMBLY PUMP UNIT 4 i 220 240 50HZ 22 004 0534 WW 004 Wa 27 000832007 LABEL IMPORTANT 8 RATING LABEL 606840004 HEAT SHRINK TUBING REB 32 000860006 HEAT SHRINK TUBING YELLOW 37 000881004 DOT MARKER ADHESIVE BACKED YELL 1 4 DIA 000891006 DOT MARKER ADHESIVE BACKED BLACK 1 4 DIA MEME NM NNNM 000881242 LOCTITE NUTLOC 242E 2 ae 0 12 7 I D 12 7 1 0 1247 I D 12 7 I D Lond El BILLINGSHURST HDENLEY ms 14 SEY APPROVED CHECKED TITLE SHEET DRAWN GENERAL ASSEMBLY PUMP UNIT Ne 2 220 240V 50HZ oF 2 THIS DRAWING IS THE COPYRIGHT UE OF DENLEY INST LTD COPIES 4 2371 LI WHOLE OR PART MUST NOT BE MADE WITHOUT THE WRITTEN CONSENT OF THE COMPANY 1993 9 o gt In use ON 004 050 TI CHASSIS ASSEMBLY PUMP UNIT 004 0548 004 636
15. 5 363 1 MSVW AMVSS3O3N SV SVN M3Q10S jO 3343 NIVN3M OL SNHL Q3MNVA S310H L 824 SIHL 3I8N3SSV OL q3sn SI ONINSCIOS N3HM SZ W3ll NISN 530 14 SL sg 30 931 5 8 3 331S NOILISOd 440 OL 135 3MV 96 W3ll NO S3HOJIIMS Tv 1 SSLON 80558 OL 3ONYOHODOv NI Q3HVd3Hd NOLUD3POBd GYIHL 1930 ASSY 1X3N LGO OC EHE S cus Sussex RHI4 9EY item Drawing _ n Des 1 004 801 PCB 1 2 004 325 LED SUPPORT 1 3 4 5 INTERGRATED CIRCUITS 6 000703065 MICRO PRO PD 8749 VERSION 4 1 IC1 7 000703017 IC 74 LS 165 4 IC2 TO 1 5 8 000703018 IC 74 LS 374 2 IC7 C20 9 000703019 IC 74 LS 04 1 IC9 10 000703021 7 WAY DARL ORIVER ULN 2003N 1 IC10 11 000703022 OPTO TRIAC DRIVER MOC 3041 3 1C11 12 13 12 000703023 IC 7404 1 13 000703030 HALL EFFECT IC UGN 3120U 1 IC14 14 000703066 IC 74 LS 125 1 22 15 000703067 IC 74 LS 139 1 21 16 5 17 000101787 2 2 35V SOLID TANTALUM 1 C3 18 000102702 10 uF 35V SOLID TANTALUM 1 C16 19 000104477 100 uF 16V ELECTROLITIC 1 C4 20 000103749 2200 uF 63V ELECTROLITIC 1 C61 21 000114424 0 47 uF 100V MIN LAYER 1 C6 22 000114418 0 22 uF 100V MIN LAYER 1 C5 23 000126124 22 pF 100V PLATE CERAMIC 2 4 2 28 100702009 0 1 25V DISC
16. The prescribed disinfection procedure is required prior to shipping the instrument 0 Labsystems e g for repair The complete disinfection procedure of the instrument is advised prior to relocating the instrument from one laboratory to another Disinfection is not required for the proper functioning of the instrument Disinfection agents Formaldehyde solution 10 Alcohol 70 Gluteraldehyde solution 4 WARNING ALWAYS USE DISPOSABLE GLOVES AND PROTECTIVE CLOTHING IN A WELL VENTILATED AREA 14 4 4 1 Disinfection procedure before transport Before the unit is transported the following procedure or something similiar should be completed 1 2 3 4 11 12 13 14 15 16 Prepare the disinfection agents 200 ml 10 Formaldehyde solution and 200 ml 4 Gluteraldehyde solution Prime the unit from the Reagent Bottle with distilled water Lift the Wash Head and fill the Prime Tray with gluteraldehyde solution Aspirate the Prime Tray using the Wash Head then reprime the Prime Tray with distilled water Remove the Plate Carrier and the Wash Head place in gluteraldehyde solution for 24 hours Empty the fluid containers Switch OFF the power and disconnect the mains cable Disinfect the outside of the unit using a wad of cotton wool soaked in 70 alcohol Place the entire instrument in a large plastic bag Ensure that the reagent containers are disconnected and open Place wad of cotton
17. pu THIS DRAWING 16 THE COPYRIGHT RE NON IAE E DANCE S DOWNES 06 DENLEY INST LTD In WHO OR P E DE WITHOUT THE WAITTEN consent oF 19 393 iri Es en e TITLE SHEET DEPT I LIST FOR DRG No DRAWN GENERAL ASSEMBLY PUMP UNIT No 2 110 120 60HZ o 2 22 004 053 60 001 ZZ SNIAVHOI 1430 buHs Wed ATHW3SSV SISSVHO AMOHIA 3OV 38 LON ISNA BO 58300 ANGI 9 SHE 151 335 z PS 800 57 VO T0 O TX XING LNVISVA 209 AOLL NOISH3A P NMOHS LON LNG 91 WALI 330 ZHOS 0 2 NOISN3A 2 B Prud TvOuoVHd SY 35010 SV HO 5 3 WOUS SNIINVIS Of Wall HUM 3901 OL SIYM 1 WOS NO MJA 15 V INVIMVA Sv LE NOLLVIN3IUO 3309 mm Zx s W EE 6l dv 6 SN SOd gt ONLLIIJ 380438 IOHODTY 142099051 3SVO NYi15 5300991 3NYHI3H ACIOd NV312 gt HUM 20 35043 1v03 Manassy gt ONIENMd 503 SL v00 335
18. usen 004 050 004 313 HEADLIFT KNOB ts ae see ae 000543901 CABLE TIE 9 10 004 648 Di Meet C C O S pem WASHER WAYE __________ 8 vem h EI BILLINCSHURST RH14 9EY E n APPROVED S DOWNES Ut gt Dimi eje be io THIS DRAWING IS THE COPYRIGHT Wa sapa F DENLEY INST LTD COPIES IN Ed HOLE PART MUST NOT BE MADE ITHOUT THE WRITTEN CONSENT OF 3 9 93 2370 HE COMPANY 1993 iss one es one en D NAPPER TITLE SHEET DEPT I LIST FOR DRG No DRAWN DENLEY 1 5 D NAPPER COUER 22 004 052 9 posco mue mu ye IDOHLA XM 38 ION ISNA Died UO TOHM 52200 n SS E dnd L EBBEH O 5 9 USM ATIC 20 AHORUJCO S 22 JANN no PHS isn SWiH 335 tO XK GOK 10030 NMOHS LON LNG a3sn S S IVI30 304 252 00 335 11815504 sv ONITINOW OL YVAN SV 03404504 SI 13911 0 M3ON3TIS 34nSN3 ONOT 3993 1914 dN3dVl 01 937130 Sv Lino uua JI3HOS M 8 50 00 335
19. mus pu 14 rrarkinigs u RR Le et de d d EDI 55 safety symbols and markings 44 424 8401 4 2444 4 4 0 nen nnns seen 7 UNPACKING uei Di RIS 12 K Keypad cer ECC 10 11 iei eu ii 36 L Lig id level Sensor ien ed 10 installatiOr niente none 19 Ligulidtrapy y uy 10 LIQUIG TRAP DOT cats aa UTR 10 37 19 23 Mails 23 35 5 35 Manifold DIP OS LIS 20 Microplate nm 27 2172 ide sen t er t ED o a Fa EEG LX EE a 22 N Number Keys t rrt ee eer dete Leone edt pe beg eel Lp 11 Number of washes cei tin creer sad cv CERERI CO AERE
20. 16 004 851 5 11 92 BINARY DISPLAY ROW POSTWASH Switch PREWASH Switch Switch Setting gt Oi i CO tQ O __ MAINTENANCE SELECT BUTTONS 1 2 4 1 2 3 3 6 7 OFF ON OFF OFF ON OFF ON ON ON OFF ON OFF ON ON ON ON OFF OFF ON OFF OFF ON OFF ON ON OFF OFF ON ON OFF ON OFF ON ON ON ON ON 8 Binary 4 Row LED cond 8 Row LED cond OFF OFF OFF OFF OFF OFF OFF OFF ON ON ON ON ON ON ONLY FOR OLD WW4 MODELS 1 SENLEY INSTRUMENTS LTD SECTION 2 MAINTENANCE 2 4 2 Pump Unit Pressure Adjustment To check the Pump Unit pressure setting disconnect the Pumo from the Washer Unit Connect a Preesure Gauge 0 15 psi range to the fluid feed pipe which would normally be connected to Manifold Block in the Washer Unit Connect the Pump Unit to the appropriate mains supply and switch ON reading of between 6 0 and 6 5 p s i should be obtained If a reading outside these limits is obtained adjustment must be performed To adjust the pressure locate the Pressure Adjustment Screw which is a chromed lock nut and screw located in a recess at the front of the pump above the Mains Outlet After releasing the lock nut the pressure may be adjusted by turning the screw turning anti clockwise will decrease the pressure turning clockwise will increase the pressure Once the pressure
21. Sl TYPE Switch 1 2 3 4 Row LED cond 52 TYPE Switch 5 6 7 8 Row LED cond Switch Setting 0 OFF OFF OFF 1 ON OFF OFF OFF 2 OFF OFF OFF 3 ON ON OFF OFF 4 OFF 5 OFF OFF 6 OFF ON ON OFF 7 ON ON ON OFF 8 OFF OFF OFF ON 9 ON OFF OFF ON A OFF ON OFF ON B ON ON ON OFF OFF ON D ON OFF ON ON OFF ON ON ON ON ON ON ONLY FOR OLD WW4 MODELS 004 851 8 11 92 9 DENLEY INSTRUMENTS LTD SECTION 2 L BINARY DISPLAY ON ROW SELECT BUTTONS I 2 4 8 Binary Sl REPT Switch 1 2 3 4 Row LED cond 52 REPT Switch 5 6 7 8 LED cond Switch Setting 0 OFF OFF OFF l ON OFF OFF OFF 2 OFF OFF OFF 3 ON ON OFF OFF 4 OFF OFF ON OFF 5 ON OFF ON OFF 6 OFF ON ON OFF 7 ON ON ON OFF 8 OFF OFF OFF ON 9 ON OFF OFF ON A OFF ON OFF ON B ON ON OFF ON OFF OFF ON D ON OFF ON E OFF ON ON F ON ON ON ON ONLY FOR OLD WW4 MODELS BINARY DISPLAY ON ROW SELECT BUTTONS 1 2 4 8 Binary Sl PAUS Switch 1 2 3 4 Row LED cond S2 PAUS Switch 5 6 7 8 Row LED cond Switch Setting OFF OFF ON OFF OFF OFF OFF OFF OFF ON ON OFF OFF OFF OFF OFF OFF ON ON OFF ON ON ON OFF OFF OFF OFF ON ON OFF OFF ON OFF ON OFF ON ON ON OFF ON OFF OFF ON ON ON OFF ON ON OFF ON ON ON ON ON ON ON uj nj UJ OY Gl i CO tO O 004 851 8 11 92 DENLEY INSTRUMENTS LTO
22. The user should be familiar with the contents of Chapter 7 on maintenance procedures that may be performed by the user For warranty and ordering information refer to Chapters 10 Ordering Information and 11 Warranty Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 8 Chapter 3 Introduction to the Wellwash 4 Mk 2 Introduction to the Wellwash 4 2 The Wellwash 4 Mk 2 is a semiautomatic microplate washer comprising pump and washer units The instrument is supplied with both 8 and 12 way wash heads four program cards and an accessory pack Your local Thermo Fisher Scientific representative can arrange instrument training at the commissioning of the instrument for extra charge if required 3 1 Intended use The Wellwash 4 Mk 2 is a microplate washer for 96 well plates It can be used in research or routine test laboratories by trained personnel to wash Microtiter microplates and strips in 96 well plate format that meet the SBS standards For verification of the entire system it is recommended that Good Laboratory Practices GLP be followed to guarantee reliable analyses Use for self testing is excluded If the analyzing performance is critical to the medical diagnosis the diagnostic test result has to be ensured with internal quality contols or with an alternative test 3 2 Principle of operation The instrument provides the following wash functions initiated through the keypad and cont
23. 210108 HOLVD 311108 SLValdSV 31108 3SN3dSIQ 438 052 s0 vo0 te0 roo 90 00 9507400 _ _ 290 500 _ _490 00_ 490 00 80 0 LLL LLLLNODDBNNOO 55 MOTA 44 O38 3AS3HOY ERA 100 I WINN 100 25 3mi8 100 ROTA CDXOVG 3AISIHGY HIMA 100 ___ 1 04471 BATS wNIeHSIVSM A 221 ame MAS MNIBHSIV3H A SZ x 3Noonis AUTBA ASS MNRIHSIVSH Sz Q i 3NOOhlS Hi Sz 3NOOIIS oe aft 656 5 o T E 2 Cz X Of SZ x 38ni 3NOOfIIS 38ni 3NO2FlS 65 5 Ht S S a SZ x 0 55 Sant _ 660 00 ALON 38 43114 ALON 336 _ 650 900 __ 50 700 BSGUVONVIS Vl X x a os Beant
24. ADPZ OZZ NOISH3A INVIMVA 31ON LNVINVA HOLIMS MOTI3A N3389 N NZ MOION 1 H 3sn3 AOR NO O vZ NMOUB T 0 0 ASSV MOTISA W3385 318VO N3380S 3m8 9 ABO lt B NN OTBA N33H9 3 831114 SNIVA INdLNO sr MOTISA N3385 fepe ME ame LZ oe i n 790 700 OION3IOS Ol PME ASSY HiVd CBLSIML ES i LTIOIN L 3AMO 1 UN OL 318 2 NOSEY NAOMB HINV3 LOAid v SUBVONVIS SNRGENIONS 53903 dHvHS eM pp uu cp HVUSVIG x 8881 30 1N3SNO2 N3ILIHA LNOHLIN LINA LON TOHA NI 831402 ALT LENI A371N30 JO SI SIMI 165 Y00 __ _____ ___ ____ ____ Srl J i 99 1 0 2 0 670 X 31 1 SV 1433 3 06 01 SAV Oc 5350 SLINN 021 0 4 LNVIYVA 4 INVIUVA A3u3 INVIHVA 30708 43849 5 SV ld32X3 3HIM 2 0 91 SJSN SLINN 9 2 022
25. S310H UO XXX eG ery UOI 830 233 0Hddv 114905 LNdINO SHIVA SLI AOLE OF OL OMY OZ SUNN AOFC NO NMOS8 cC O vZ 03183 38 7300 ATMv3 2345 iH34a4nq2 S OL SQv31 2 HO 1412 3 S3SA G2 E ced T DS m TE c 2 2 FISY 1v NOIO3HNO2 dJOHINOO Oil ang 5 ES rs SNIVW MOTI3A N3339 m A POM lt m 3019 335 G8VOS 1091405 MOT13 A N3335S LAdNI SNIYN x 900 SOSVONYIS OMIN3HIONS n2 53003 4 5 uo 57778 JONYJECOOY se s c 5 TEN s BM A 3 43 1839 1 559 we wip mem Bu 22 B Tao es M GIC Ti i Pai Cin r OIN IO XU IS quee i iu SF EET
26. WW OO06 2L2 2 12 way Programme Card 1 and 4 washes for ww4mk2 WW O06 2C1 3 12 way Programme Card 2 and 3 washes Denley WW006 3L2 2 12 way Programme Card 2 and 3 washes for ww4mk2 WW006 3C1 3 nx cL Wash He d WW 004 11 8 way Programme Card 1 and 4 washes Denley WW004 12L 1 8 way Programme Card 1 and 4 WW006 12L2 2 8 way Programme Card 1 and 4 WW 006 12C1 3 8 way Programme 2 and 3 washes WW O04 13L 1 8 way Programme Card 2 and 3 washes WW006 12L2 2 8 way Programme Card 2 and 3 washes WW 006 12C1 3 WW004 20 Overflow C WW 004 21 2 litre Wash or Waste Bottle with plain cap WW004 22 Adaptor Plate for Flexible microplates eese WW004 27 Remote Pump Extension WW 004 28 Spare O ring pack 5 of each RE teu WW004 29 4 5 litre Bottle with plain beri o WW004 30 4
27. imo PMID Of Ouest Sra n ome 7 011 Cos crat wee usnm lint Jesu SEIN 129 900 JIBNISSY 37872 031915 SSTING 20 94 38 SQvi 532188255 33870 142353 Jam MOTIS4u3382 L3UQV3H 3553 a 5 6 NMOS 3 suem 7 P Ju M OTI31 83389 03151 7634 FA EE MOTTE u SAMI 2380 l MOTIJA N3389 2 SV EE SNIV W3FOIO SSNYNI I indino 2 9 O1134 N3385 03151 1 3 BOTIA N338D Ov3H GION3 IOS 01 JUHM HOLOW ino 33 00 01 137014 31872 89 2 HiNY3 Aval B 800 Staas aceti 13023 eem D i 27 nene ORD sOilYOes Teen Dee 7 o Suir 1357 Ss LAS te EAN E r ESAE awe LEN eve M TU pes mr Deseo ez ke NL MM IiROHUA SWA LON Lena Deg
28. CATCH BOTTLE CAVITY 110 004 1224 5 50 019 SILICONE TUBE _ 146 004 3234 PU MOULDING PUMP UNIT Se 0Gosoroz ELASTIC 934 330 BOTTLE CAP MOD 5 000821006 000904078 WASHER WAVE ER WR S 2 000803008 NUT FULL HEX Emi w 24 25 SILICONE SEALANT ____________ THIS DRAWING IS THE COPYRIGHT 2 12 87 48 aif QA wor ae WITHOUT THE WRITTEN CONSENT 1 1387 El 1 amp amp LONG LONG Win lt TITLE SHEET DEPT I LIST FOR DAG No DRAWN GENERAL ASSEMBLY PUMP UNIT Me 1 DN PJF 110 120V 60HZ o 2 22 004 0538 USED WW 0048 DRAWING No TITLE 2 000882010 LABEL BOTTLE FULL 27 000892007 LABEL IMPORTANT 8 000900020 RATING LABEL H lt HEAT SHRINK TUBING RED Saal 12 7 I D 000840005 HEAT SHRINK TUBING BLUE 12 12 7 I D 000840006 HEAT SHRINK TUBING YELLOW 3 000840007 HEAT SHRINK TUBING BLACK DIA 000891006 DOT MARKER ADHESIVE BACKED BLACK 1 4 DIA 1 000881242 LOCTITE NUTLOC 242E 000840004 1 12 12 7 I D a CO N BILLINGSHURST BDENLEY s ae muxr L
29. Q 8 D p ua Saa 10 11 NOTE Spare parts that considered as Recommended Spare parts to service offices have in theyre stock are marked with Document Release 004 006 100 3 0 2 ThermoFisher SCIENTIFIC WELLWASH 4 2 SERVICE MANUAL Spare parts POS ITEM CAT 1 Carriage ASSN 004 0841 2 2 Membrane panel u 004 644 3 Washer track assembly See page 4 004 052L2 4 Control assembly 230V See page 6 006 053AL2 Control assembly 115V See page 006 053BL2 5 Pump unit assembly 230V See page amp 006 052AL2 Pump unit assembly 115V See page amp 006 052BL2 Document Release 004 006 100 3 0 3 ThermoFisher SCIENTIFIC WELLWASH 4 2 SERVICE MANUAL WASHER TRACK ASSEMBLY Document Release 004 006 100 3 0 4 ThermoFisher SCIENTIFIC WELLWASH 4 2 SERVICE MANUAL Washer track assembly Spare parts POS ITEM CAT 1 Track 004 055
30. See Section 6 The protection is likely to be impaired if for example the instrument 1 shows visible damage fails to perform the intended functions has been subjected to prolonged storage under unfavourable conditions has been subjected to severe transport stresses SERVICE WARNING VOLTAGES DANGEROUS TO HUMAN LIFE ARE PRESENT IN THIS INSTRUMENT BEFORE REMOVING ANY COVERS DISCONNECT THE INSTRUMENT FROM THE POWER SUPPLY 6 1 Warranty Statement This instrument is warranted against defects in workmanship or material for a period of 12 months from the date of purchase Any fault or part found to be defective will be repaired or at the manufacturers option replaced without charge for labour or material on condition that the instrument 1 Has been used only the supply voltage as stated on the rating plate attached to the instrument 2 Has not been tampered with or been subject to misuse or accident and that any ecommended user maintenance has been completed on schedule 3 Has not been repaired or modified by any person other than an authorised Manufacturer s employee or agent 6 2 Service Contracts It is strongly recommended that this instrument is regularly maintained and serviced every six months on a contract basis by the Manufacturer s trained service engineers This will ensure that the product is properly maintained and gives trouble
31. Warning Remove any microplate still on the plate carrier Dispose of all microplates and strips as biohazardous waste The wash reagents may contain buffer salts which can crystallize when the washer is not in use This may cause clogging of the wash aspirate tips To avoid this the following procedures should be followed 6 7 1 Daily shutdown procedure When the last wash cycle of the day is complete switch the power OFF through the switch at the rear of the pump unit Fill the priming reservoir of the plate carrier with distilled water and lower the wash head tips into the priming reservoir Do not detach the tubes unless you first open the wash bottle cap slightly to release the pressure Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled aqua a mild detergent SDS sodium dodecyl sulfate or soap solution If you have spilt infectious agents on the instrument disinfect with 7096 alcohol or other disinfectant See the Wellwash 4 Mk 2 User Manual Section 7 3 Last of all put the dust cover on 6 7 2 Long period shutdown If the washer is not likely to be used for a long period you should flush the system through with distilled water prior to carrying out the DAILY shutdown procedure Empty and clean the waste bottle and check that the liquid trap is dry When the washer is to be used after a prolonged shutdown you should prime the system Section 6 3 at least four 4 times p
32. contaminated sufficiently to reduce its efficiency it should be replaced Part No 000 727 008 available from the Service Dept Re assemble by reversing the above steps IMPORTANT Care should be taken when re fitting Moulded Cover to avoid crimping of any tubing which may affect the Pump Units performance 004 851 5 11 92 DENLEY INSTRUMENTS LTD SECTION 2 __ MAINTENANCE 2 1 5 Indexing Mechanism Check Periodic inspection of the Indexing Mechanism is recommended to ensure correct operation although replacement is seldom required To inspect the Indexing Mechanism remove the Washer Unit cover by taking out 6 fixing screws on the underneath and 2 fixing screws at the rear of the unit Lift the cover clear of the chassis The Indexing Mechanism is located on the right hand side of the chassis The mechanism should be checked for any serious wear on the pulleys or on the sliding section and for the correct tension of the Drive Belt It is important that the position of the Opto comb in relation to the Sliding Section is correct to provide central location of the Wash Head Tips in the Microplate Adjustment is achieved by loosening the locking nuts on the Opto comb moving it to the required position using the slots and re tightening tbe lock nuts It is also important that the Drive Belt is correctly tensioned such that the sliding bracket moves smoothly while at the same time providing positive positional accuracy Tension of
33. Ieri 32 Spare parts l aedes ner EE teret ER 56 Specifications general at Bie Ril eh at igi ene 54 55 DII 11 N 26 SWITCH CAN 31 T Tips UN CLOGGING Mh ce 42 Transport darmage 7 RR REO 14 Transport locking 5 10 13 16 Transport packagB eir n REESE in ERR Uti e 12 14 RD ES 48 Troubleshooting S ao ER E eek aes oe ve se ea 49 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 61 Chapter 12 Index Tubes E 18 unpinchilg ego 41 Two way SWITCHES uuu luan 31 Qu 12 Using after prolonged 1 II enne 34 Vacuum fail
34. Warranty limitations cet a eae 58 lin 59 Glossary and Abbreviations 63 Keywords for web a n n 63 ea RD SEEN REDE 63 Wellwash 4 Mk 2 Quick Reference Guide esee 64 Certificate of Decontamination 65 Wellwash 4 2 Feedback Form 66 Addresses u EE 67 5 68 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 List of Figures Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 41 4 2 5 1 5 2 53 5 4 5 5 5 6 5 7 5 8 5 9 5 10 5 11 5 12 5 13 5 14 5 15 5 16 6 1 6 2 6 3 7 1 7 2 7 3 7 4 7 5 7 6 Z 78 7 9 7 10 7 11 List of Tables Table 5 1 Table 5 2 Table 6 1 Table 6 2 Table 6 3 Table 6 4 Table 6 5 Table 6 6 Table 6 7 Table 7 1 Table 8 1 List of Figures and List of Tables Wellwash 4 2 microplate washer 995595999555 555555595 11 Keypad ied bee TRA 12 Accessories included with the instrument 14 Spare TUBING screen na Vv
35. accessory pack which comprises Operators Manual Spare Fuses Spanners Mains Supply Cable Spare Tubing Allen Key Declogging Tool Spare O Rings 6 2 UNPACKING AND INSTALLATION 2 1 Unpacking and Inspection for Transit Damage The Wellwash 4 comprises two units which are packed in the same carton Place the carton on the floor and open the top This will reveal an inner carton opening this will reveal the two further packs approximately half the size of the carton One pack contains the Pump Unit Figure 1 1 the other is a rectangular tube containing the accessories underneath which is a further pack containing the Washer Unit Remove accessory spares pack from the top of the Washer Unit Lift out the packs and open them to reveal the Accessories Pump and Washer units Figure 1 2 Lift out the Washer and Pump Units removing the shaped packing pieces from around the Washer and place them on a flat level surface Remove the units from their plastic bags and the packing piece from underneath the Wash Head Figure 1 7 Remove the plastic bag from around the Head Assembly Figure 1 10 NOTE Retain the original packing materials for future use in the event that the unit is shipped to another location or returned for service This package is customed designed to assure safe shipment so please do not substitute alternative packing materials Check for visible signs of damage If damage is found retain the packing material request
36. but the instrument remains ready for use ROW SELECT 1 12 push buttons These are momentary action switches that select the last row to be washed in any chosen program sequence An audible tone will sound when pressed to confirm operation ROW SELECT indicators These yellow LEDs located to the right of the ROW SELECT push buttons when illuminated indicate the rows selected for washing START push button This momentary action switch initiates the wash cycle An audible tone will sound when pressed to confirm operation RESET push button When pressed the RESET button will reset the washer to its pre programmed state An audible tone will sound when pressed to confirm operation POWER indicator This green LED is located at the bottom left of the keypad and when illuminated indicates that the POWER switch is ON VAC FAIL indicator A yellow LED which when flashing indicates that the waste bottle is full or that there is a vacuum failure Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 12 Chapter 5 Installation 5 Installation 5 1 Installation check list The section on installation will contain an outline of the points mentioned in the check list below Table 5 1 Installation check list Tick OUD Item Unpack the Wellwash 4 Mk 2 instrument carefully Refer to 5 2 1 Keep the original packaging and packing material for future transportation Check the d
37. sabotage war riot or any other occurrence of the character listed above e Refurbished products which are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connection with the use of the product or other indirect damages Full warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer This document acts as warranty certificate 11 1 Warranty limitations Consumables are not included in the warranty Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Chapter 12 Index A AGCOSSOFIBS ciini aet ea pane IRR aquqa a E FATE REFIERE 56 14 12 5 m 13 Applicat OM 2 65 AUIS 30 Bottle Caps 10 a IL aa ed LEE 10 iui ER CLE 37 replacement Interval RM 36 BUON mm 11 C Certificate of Decontamination
38. 000848013 54 000848008 55 000337598 56 000336696 57 000412403 88 000412784 59 000713005 60 000713013 62 000791008 63 000791009 64 000794004 65 000730001 66 000794002 70 000401986 71 000848010 72 000606771 73 000402181 74 3 75 000827005 77 000702003 80 000399394 86 000802049 87 000802050 90 000802259 93 000803008 96 000804076 Drawn D Napper Checked Approved J Hazell A Partridge AUD WARNING OEVICE 22094 38 12V 3mm LED YELLOW TELEFUNKEN THLY 4614 3mm LED GREEN CMD 5474 C 3mm LED YELLOW CMD 5374 C KEYBOARD SWITCH RED 2 WAY DIL SWITCH FUSE 2 250V BSI KITE MARKED FUSE CLIPS 3 WAY CONNECTOR 4 WAY CONNECTOR 2 WAY TERM BLOCK SMKD 53 2 5 08 3 WAY TERM BLOCK SMKD 53 3 5 08 CARD CONNECTOR HEATSINK RIGHT ANGLED HEADER 10 WAY SOCKET 40 WAY LED SPACER SPACER 20mm TRANSISTOR PAO FISH BEADS RED HELSYN SLEEVE CAPACITOR 0 091 uF 700V SILICONE SLEEVING tmm BLACK SCREW M3 x 8 PAN HEAD POZI SCREW M3 x 10 PAN HEAD POZI SCREW M3 x 30 CSK HEAD POZI NUT M3 FULL HEX WASHER M3 WAVE DENLEY INSTRUMENTS LTD COPIES IN 6 16196 _ 5642 WHOLE OR PART MUST NOT MADE WITHOUT THE WRITTEN CONSENT OF THE COMPANY 1996 4 14 1295 mh N Sel 10 36 Q N 10mm ren Gaia d ET sess Title CONT
39. 1 INTRODUCTION 1 1 Description of the Instrument 12 Safety and Power Requirements 1 3 Specification UNPACKING AND INSTALLATION 21 Unpacking and Inspection for Transit Damage 2 2 Site Requirements 23 Installation Procedure 2 4 Operational Check OPERATING PROCEDURE 34 Controls and Indicators 3 2 Operating Procedures 3 3 Shut Down Procedure OPERATING PRECAUTIONS AND LIMITATIONS 41 Before Operation 4 2 Cleanliness 4 3 1 Operation 4 4 Disinfection Procedure HAZARDS 5 1 Liquid Reservoir IMPORTANT 5 2 Electrical 5 3 Mechanical and Environmental 5 4 Defects and Abnormal Stresses SERVICE 6 1 Warranty Statement 6 2 Service Contracts 63 Maintenance and Service 6 4 Fault Finding 6 5 Fuse Replacement 6 6 Service Requests Page amp WW CO 20 20 1 INTRODUCTION This manual gives the installation and operating instructions for the Wellwash 4 Microplate Washer together with the maintenance procedures that may be performed by the user The manual should be read in its entirety prior to any attempts to operate the Washer Special attention should be paid to sections 2 3 4 and 5 1 1 Description of the Instrument The Wellwash 4 is designed to wash coated wells in a 8 x 12 microplate It comprises of a Pump Unit and Washer Unit Fig 1 which are connected by power air and liquid lines The Pump Unit consists of a double headed va
40. 5 litre Bottle conversion kit including dispense bottle WW 004 31 4 5 litre Bottle conversion kit including aspirate bottle WW004 32 8 way Half Area Wash a WW004 34 2 litre Wash or Waste Bottle with quick release cap WW004 35 2 litre Waste Bottle with quick release cap N03283 4 2 litre Waste Bottle with quick release cap and level sense assy 1514313 4 Waste level sensor dsserti y uuus 1514319 4 Second Dispense Bottle WW004 39 Wash S WW004 48 Programmable Programme Card WW 006 10C1 2 litre labelled Wash or Waste Bottle WW934 1 1 Used in instruments manufactured before the serial number 004 078 Document Release 004 006 100 3 0 11 ThermoFisher SCIENTIFIC WELLWASH 4 4Mk2 SERVICE MANUAL 2 Usedin instruments manufactured after the serial number 004 078 3 For WW4mk2 4 For instruments made after s n 006 9 4693 Document Release 004 006 100 3 0 12 ThermoFisher SCIENTIFIC 9001 so brew s IWA 38 LON ISNA Dvd 0 63000 3HSvA AI8W3SSY SMAO t XU US AIGO 30 MOMI 3HL OMM SHL 250 00 ZZ
41. 9 q 08 31 81 134305 104100 Lu D INVIUVA NO GaLVIOSI d3H _ q LNVIHVA NO 831 7061 q iNVIUVA 039 d 4 038 __ LNVIHVA NMOHB 3H Pe dMWfid 77 HOLIMS SNIVM SLINA AOTT NO 3HIM 0 01 SLINN NO SHIM NMOHS Z 0 vC 6831114 38 S 300H ANIT SYH 9345 lN3uu 9 M0T13A N3309 9 0 81 9MV8T 9 INVIUVA 0 91 _ M0113A N33HU9 700 IARA J 2 1564 00 9001 owonoo 0 Su iguana JOVA 38 JON 150 3409 USN ATWGU 9 OMA SML op Wvuovid SNIBWYId _ O318W3SSv N3HM dvO NO 93410709 HOIVW OL 9985 Sl 30 3007109 dvd ISNIVOV dn LING SI SdvO 37008 1d39X3 SON3 38ni 08 01 9313 5 9100709 31ON 000 0 wow UD XXX T0 Xx SOF AOL GZ v00 335 ONIGWN Id NINOS 503
42. AUD WARNING DEVICE EB 22094 38C 12V 1 AWD1 RS 249 794 52 000848015 3mm LED YELLOW TELEFUNKEN THLY 4614 4 LD12 13 14 16 53 000848013 3mm LED GREEN CMD 5474 C 1 LD11 54 000848008 3mm LED YELLOW CMD 5374 C 14 LD1 TO LD10 17 LD18 19 20 55 000337598 KEYBOARD SWITCH RED 1 549 56 000336696 2 WAY DIL SWITCH 1 0 1 57 000716005 FUSE 2 250 UL CSA 239 1 51 58 000412784 FUSE CLIPS 2 51 59 000713005 3 WAY CONNECTOR 1 PL1 60 000713013 4 WAY CONNECTOR 1 PLS 61 62 000791008 2 WAY TERM BLOCK SMKD 53 2 5 08 3 1 2 4 63 000791009 3 WAY TERM BLOCK SMKD 3 3 5 08 1 TB3 64 000794004 CARD CONNECTOR 1 EC1 65 000730001 HEATSINK 1 FOR REG1 66 000794002 RIGHT ANGLED HEADER 10 WAY 10 36 PL7 67 68 69 70 000401986 IC SOCKET 40 WAY 1 71 000848010 LED SPACER 4 FOR LD12 13 14 16 72 000606771 SPACER 20mm 2 73 000402181 TRANSISTOR PAD 3 FOR SCR1 3 74 N3 FISH BEADS 8 FOR R45 75 000827005 RED HELSYN SLEEVE 17 76 77 000702010 CAPACITOR 0 39 uF 300V 1 C21 78 NOTE 79 TEST PCB BEFORE FITTING C21 80 000399394 SILICONE SLEEVING 1mm BLACK 10mm FIT TO C19 81 82 83 84 85 86 000802049 SCREW M3 x 8 PAN HEAD POZI 1 87 000802050 SCREW M3 x 10 PAN HEAD POZI 2 88 89 90 000802259 SCREW M3 x 30 CSK HEAD POZI 2 91 92 93 000803008 NUT M3 FULL HEX 5 94 95 96 000804076 WASHER M3 WAVE 5 d 1 9 APR 1996 98 99 Bugi dJ t00 THIS DRAWING IS THE COPYRIGHT OF d L L IT C
43. Delay Indoor use Operating range Full specification range Storage temperature range Transport temperature range 9096 max non condensing Max pressure on vessel 100 110 120 Vac 2 0 5 A 250 V UL 1986 Time Delay 2 x 2 0 A 250 V UL 198G Time Delay 5 to 40 15 C to 25 10 C to 70 C packed in transport packaging 40 C to 70 C packed in transport packaging 7 psi 0 5 bar self limiting Plate type Containers 96 well plate Material HDPE high density Wash bottle capacity Waste bottle capacity Additional wash bottle capac polyethylene 2 liters 2 liters ity 2liters Fluid consumption Residual aspiration volume Wash fluid consumption Priming consumption approx 150 ml complete microplate approx 15 20 ml lt 5 ul per well using 0 02 Tween solution Dispensing precision 5 using 0 0296 Tween solu tion Wash heads 8 way and 12 way Material POM polyoxymethylene polyacetals Wash program cards 1 4 washes Programmable card Wash time Volume 0 750 ul Washes 1 15 Soak time 0 10 minutes Pause 0 4 minutes 120 s x 350 ul with 12 way wash head 165 s 3 x 350 ul with 8 way wash head Thermo Scientific Wellwash 4 Mk 2 User Manual Re v 2 3 Cat no 1507340 55 Chapter 9 Technical Specifications 9 2 Safety specifications Safety performance EN 61010 1 1993 2 1995 610
44. ET COD RETE E TEE TRA 33 15 Operational Check 23 56 Ovettilling Of wells ore 49 P PACKING ouo eie CE cime M CO A en T cT 47 OP 46 PETERE his ER NE ERR ERR 13 Pa se between Washes iiie tenia a we da co Lara Ev qawa 33 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Chapter 12 Index Pinchr eet ve teta ca br ERG 10 19 28 uem 17 oc IEEE 41 IMEEM 9 34 ETE 34 HQ Ea A 10 26 28 MOL MOVING IMPER 49 Spillages a Ae 36 QUEEN Ra UA 31 ROWED 23 Power MGIC ATOR rne hapa i Fee X Ear GR e I ae RT PY E D ty 11 eee bes rho hr nm Co ed Pra E vod
45. Rev 2 3 Cat no 1507340 40 Chapter 7 Maintenance 6 Letthe red coded tube remain connected to the bottle cap and take the bottle out unscrew the liquid trap bottle cap and empty the bottle Fig 7 5 Opening the liquid trap bottle 7 Close the bottle Make sure the end of the red coded tube is about 2 cm deeper inside the bottle than the thin tube will be Put the bottle back to its place but don t push it yet all the way down 8 Insert the thin tube back through the liquid trap bottle cap so that the mark on the tube Fig 7 4 item 1 is at the level of the cap 9 Push the bottle all the way down Push the thick vacuum tube as far as it goes Its end should now be approximately at the same level as the red coded tube s end 10 Slide the cover back down and screw the black screw back on You can pull the red coded tube slightly out to make sure the cover is tightly on Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 41 Chapter 7 Maintenance 7 1 8 Pinch valve Occasionally the tube passing through the pinch valve becomes permanently closed i e it will not return to its normal shape when the valve opens while in use This tends to occur after extended periods of non operation Release the pressure in the tubing by opening the wash bottle cap slightly To unpinch locate the valve under the wash arm assembly Press the black top of the valve to release the tube and pull the tube gently th
46. Switch See 2 4 2 Washer will not Prime Programme Card missing Insert Programme Card 004 881 5 11 92 10 JENLEY INSTRUMENTS 179 2 PROBLEM POSSIBLE CAUSE Poor aspiration Air leaks Constricted tubing Pump Failure MAINTENANCE ACTION Check bottle caps for leaks amp tighten as required Check tubing for kinks Check Pump Unit is switched ON and is correctly connected to Washer Unit Feed tube in Pinch Valve constantly pinched No delivery of wash reagent into microplate Air leaks at Wash Reagent bottle cap Tubing leaks Dispensing Head blockage Pump Filter blocked Pull tube through and reshape or replace See 2 1 1 Tighten cap replace gasket if required Check tubing amp tube connectors Inspect Dispense Head See 2 1 3 Inspect Pump filter See 2 1 4 Unequal dispense or aspirate of wash reagent Microplate incorrectly positioned on Plate Carrier One or more Dispense Tip blocked Pump Unit pressure incorrectly set 204 851 5 11 92 11 Check position of Microplate Inspect Dispense Head amp clean as required See 2 1 3 Re set Pump Unit pressure See 2 1 2 5ENLEZY INSTRUMENTS LTD SECTION 2 MAINTENANCE 2 3 Removal Replacement Procedures The following paragraphs describe removal and replace
47. also Section 8 2 Service request protocol 7 41 Packing The Wellwash 4 Mk 2 comprises two units that are packed in a single box For packing the washer and pump units follow the procedure below 1 Place the box on the floor and open the top Put the bottom part of the package shock absorber at the bottom of the box 2 Wrap the washer and pump units into plastic Drop them into their own places onto the bottom part of the package shock absorber 3 Putthe top part of the package shock absorber into the box Ensure correct positioning and the correct location for the manifold support piece 4 Putthe bottles into Minigrip plastic bags and drop them into their own locations in two corners of the box 5 Putthe two small carton boxes onto the top part of the package shock absorber DO NOT place the small boxes on top of each other but place them instead into their own specific locations 6 Putthe rest of the items user manual labels notice packing list etc into a Minigrip plastic bag and place it into its own position on the top part of the package shock absorber 7 Close and seal the box 7 5 Disposal of the instrument Appendix B on decontamination Also wash and decontaminate the Warning Decontaminate the instrument prior to disposal See Section 7 3 and bottles prior to disposal Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equi
48. damaging Indicator LED s 2 4 Calibration Test Procedures WARNING VOLTAGES DANGEROUS TO HUMAN LIFE ARE PRESENT IN THIS INSTRUMENT BEFORE REMOVING ANY COVERS DISCONNECT THE INSTRUMENT FROM THE MAINS SUPPLY The following paragraphs describe adjustments tests and calibration procedures that may be performed on this unit 2 4 1 Control Logic PCB Calibration Testing The following section describes how to use the self test functions built into the Microprocessor IC To test calibrate the PCb remove the Washer Unit cover by taking out 6 fixing screws on the underneath and 2 fixing screws at the rear of the unit Lift the cover Clear of the chassis leave all electrical connections to the PCB intact including the keypad ribbon cable connection The basic test procedure is outlined below and is explained in further detail in the following sub sections 004 851 5 11 92 DENLEY INSTRUMENTS LTD L3 SECTION d MAINTENANCE Basic Test Procedure Only for old WW4 1 Set switch 1 of the Dual in Line DIL switches 051 to ON 2 Insert Programmable Switch card into Unit all hexadecimal switches on the Card should be set to O 3 Apply mains power to the Washer Unit and press SW9 on the PCB all indicator LED s should flash and the Audible Warning Device AWD should sound 4 Pyess the Start key on the keypad Insert a small Screwdriver or similiar tool into the Sl TYPE and 52 TYPE switches on the Program Card by
49. ead ree ERR 36 Immediate Hie 37 A a 37 Reagent waste bottles Y 37 trap bottler cee ae Rene n eA A edis 38 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 7 1 8 7 1 9 7 1 10 7 2 7 3 7 3 1 7 4 7 4 1 7 5 8 1 8 2 8 3 8 3 1 8 3 2 8 3 3 8 4 8 5 8 5 1 8 5 2 8 6 9 1 9 2 9 3 10 10 1 10 2 11 11 1 12 13 13 1 13 2 Appendix A Appendix B Appendix C Appendix D Table of Contents PING Valve teo etr ie AT ct e e 42 tips e ee Ert a 43 F sereplacement endet tree etit s eso Sachse asa uk Tea 44 Disposal Of materlialguuu u en nnne a nennen nns 46 Decontamination 46 How to decontaminate the 1 1 46 HOW to pack Tor SERVICE ul 47 ttd P I ee 48 Disposal of the 2 U nnne nnns nennen nnne 48 Troubleshooting 49 Troubleshiootirg gulde ite ten enar 49 Service request 1 1 2 1 04 ener enne 52 Limita
50. free service For details please contact the Service Department See Section 6 6 6 3 Maintenance and Service Routine maintenance and service procedures that may be performed by the user are described below For any other service problems refer to the authorised service See Section 6 6 6 3 1 Routine Maintenance Clean the instrument as often as necessary It is recommended that the instrument is wiped with a soft cloth moistened with a mild detergent solution Spillages of liquid on the carriageway should be wiped off promptly as this may cause the Plate Carrier to drag during operation 6 3 2 Pinch Valve Service Occasionally the tube passing through the pinch valve becomes permanently closed i e it will not return to its normal shape when the valve opens in use This tends to happen after extended periods of non operation 18 unpinch locate the valve under the stainless steel assembly holding the Wash Head Pull the tube gently through the pinch valve a few millimetres the valve will now pinch in a new position If the Washer is now run liquid will flow normally It may be necessary to replace the tube after extended periods of use due to fatigue 6 3 3 Unclogging Tips It is possible that the dispense aspirate tips of the Wash Head may become clogged with salt deposits To clear them switch OFF the Washer lift up the Wash Head Assembly and unclog the tips using the small declogging tool provided 6 4 Fault Finding
51. has been correctly set re tighten the lock nut 2 4 3 Vacuum Fail Switch Calibration Testing To check the function of the Vacuum Fail Switch connect the Pump Unit to the Washer Unit connect to the appropriate mains supply and switch ON To set the Vacuum Fail Switch loosen the aspirate bottle cap manually lift the Wash Dispense Head and insert a small screwdriver into the silicone guide tube located in the vertical slot in the moulded case immediately behind the pinch valve By pushing the screwdriver fully home location will be felt in the Vacuum Fail Adjustment screw Turn the adjustment screw Clockwise until the Vac Fail LED flashes Turn the mains power off Tighten aspirate bottle cap then turn mains power on Vac Fail LED should now be extinguished Perform a normal wash run and while the unit is in motion loosen the aspirate bottle cap the Vac Fail LED should illuminate to confirm correct setting of the Vacuum Fail Switch Attention in all WW 4 mk units the vacuum fail switch is in the back of the pump unit You can adjust it by inserting a Phillips screw driver into white plastic hole in bottom left corner at the back of the pump unit 004 851 5 11 92 INSTRUMENTS LTD 18 SECTION 3 3 2 Tools Equipment 004 26 GR2 112 BS400 109 GR2 152 000 960 003 4004 010 Pressure Gauge Vacuum Flow Gauge 004 851 5 11 92 Declogging Tool 3mm Allen Key 2 5mm Allen Key 2 0mm Allen Key S
52. list the Transportation discrepancy report the User Manual DO NOT touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 13 Chapter 5 Installation Fig 4 1 shows the pump and washer units in their entirety Lift out the washer and pump units removing the shaped packing pieces from around the washer and place them on a flat level surface To lift the instrument put your fingers under the bottom on both sides and lift it with your back straight The net weight of the instrument is 10 kg 22 Ibs Caution When unpacking the instrument it is recommended that two people lift the instrument together taking proper precautions to avoid injury Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Note Before use remember to remove the transit screws at the bottom of the instrument and save them in the adjoining screw holes marked with the text Transit screw storage Remove and save the pinch valve holder until needed for future transportation of the instrument 5 2 2 Check
53. microplates are being used make sure that there are no missing wells in the rows to be washed and that all the wells are at the same level If you press the RESET button on the keypad at any stage this will stop the immediate washing sequence and reset the system to its pre programmed state Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 28 gt gt gt Chapter 6 Operation Caution DO NOT smoke eat or drink while using the Wellwash 4 Mk 2 Wash your hands thoroughly after handling test plates Observe normal laboratory procedures for handling potentially dangerous plates Use proper protective clothing Use disposable gloves Ensure that the working area is well ventilated Caution Never spill fluids in or on the equipment Prevent any liquid from entering the instrument Caution Immediately wipe away spilled liquids from outer surfaces to prevent damage and wipe over with a mild detergent Caution Keep the instrument free of dust and other foreign matter Clean the plate carrier and the plate carrier slideway and keep them dry to prevent jamming 6 3 Priming procedure Position the plate carrier on its slideway with the priming reservoir to the rear under the wash head see Fig 5 13 Press the PRIME button The wash head will lift and the pinch valve will open for one second allowing wash fluid to flow into the priming reservoir The wash head will then lower itself to empty the priming res
54. over the plate Note The times currently available are subject to approval and may be altered 1 to suit individual customer requirements Table 6 5 Pause in seconds between each washing cycle Setting 0 1 2 3 4 5 6 7 Strip wash mode 0 1 2 3 4 5 6 7 seconds Plate wash mode seconds Setting 8 9 A B C D E F METE 9 10 15 20 45 60 seconds Plate wash mode 50 60 75 85 105 120 180 240 seconds WASHES Set the number of washes to be performed before a soak period Table 6 6 Table 6 6 Number of washes before soaking Setting 0 1 Number of washes 0 1 2 Setting 8 9 A B C D E F Number of washes 8 9 10 11 12 13 14 15 No wash only aspirate VOLUME Set the volume dispensed during washing Table 6 7 Table 6 7 Washing volume in Setting 0 1 2 3 4 5 6 7 Washing volume ul 0 50 100 150 200 250 300 350 Setting 8 9 A B C D E F Washing volume ul 400 450 500 550 600 650 700 750 Note The dispense volume is less than the wash volume to avoid overfilling the wells Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 34 Chapter 6 Operation 6 6 Resetting If you press the RESET button on the keypad at any stage this will stop the immediate washing sequence and reset the system to its pre programmed state 6 7 Shutdown procedure
55. slideway so that the reservoir is under the wash head Fig 5 13 1 Wash head 2 Priming reservoir 3 Plate carrier Fig 5 13 Correct position of the plate carrier Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 22 Chapter 5 Installation 5 3 2 Wash head height To accommodate the differences in size and volume capacity of manufacturers microplates the height of the wash head is adjustable This means that a positive meniscus can always be obtained without an unnecessary waste of wash reagent The wash head is factory set at a nominal height to suit the most commonly used microplates Should adjustment be required follow these instructions 1 Make sure the wash bottle is empty to facilitate the adjustment Start a simple wash routine and wait until the wash arm assembly starts to slide backwards Turn OFF the instrument from the power switch at the back of the pump unit The wash head is now in the raised position A stainless steel head lift rod is located on the left hand side of the washer unit immediately above the keypad There is a domed nut at the top of the rod with a locknut underneath Perform the adjustment using the two spanners 5 5 6 0 supplied with the instrument Fig 5 14 Loosening the locknut with spanners 4 Loosen the locknut Fig 5 14 by keeping the 6 0 spanner stationary while turning the 5 5 spanner clockwise Adjust the height by rotating the domed nut cl
56. subsequently shifted out by clocking the CLK inputs both of these inputs are under direct control of the microprocessor via Ports 10 and 11 Switch device 051 is connected to Input Ports 14 to 17 004 851 5 11 92 9 DENLEY INSTRUMENTS LTO SECTION 2 QI WARNING VOLTAGES DANGEROUS TO HUMAN LIFE ARE PRESENT IN THIS INSTRUMENT BEFORE REMOVING ANY COVERS DISCONNECT THE INSTRUMENT FROM THE POWER SUPPLY 2 1 Preventative Maintenance We recommend that the listed items should receive periodic preventative maintenance to the following schedule 1 Fluid Feed Tube Washer Inspect 6mths 2 Pump Unit Pressure Check 6mths 3 Wash Heads Inspect 6mths 4 Pump Unit Filters Inspect l2mths 5 Indexing Mechanism Check l2mths 2 1 1 Fluid Feed Tube Washer Inspection Occasionally the tube passing through the pinch valve becomes permanently closed i e it will not return to its normal shape when the valve opens in use This tends to happen after extended periods of non operation To unpinch locate tbe valve under the wash head arm Pull the tube gently through the pinch valve a few millimetres The valve will now pinch in a new position If the Washer is now run fluid will flow normally If the tube has permanently distorted replacement will be required to ensure correct dispensing To replace the tube lift the Wash Head and disconnect the tube from the Manifold Block remove the tube from the Pinch V
57. sure the ambient temperature range is between 5 C 41 F and 40 104 F preferably between 15 C 59 and 25 C 77 e Make sure relative humidity is between 10 and 90 non condensing Note The use of the instrument in strong direct sunlight or next to radiators and other heat sources may markedly increase the temperature prevailing at the instrument This should be taken into account when deciding where the instrument should be located and operated The Wellwash 4 Mk 2 does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Warning DO NOT operate the instrument in an environment where potentially damaging liquids or gases are present 5 2 5 Things to avoid DO NOT smoke eat or drink while using the Wellwash 4 Mk 2 Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Be sure the working area is well ventilated Never spill fluids in or on the equipment Only use the instrument for its intended purpose Refer to Section 3 1 5 2 6 Technical prerequisites The washer and pump units require no special site preparations A normal laboratory bench close to a mains supply socket is quite suitable The net weight of the instrument is 10 kg 22 Ibs Check that the voltage label on the rear panel of the pump
58. that the Head engages in the slots provided and that the O Rings in each End Cap are in position and undamaged To maintain correct and even washing it is also important that the Wash Head is set at the correct height above the microplate being used To check the height of the Wash Head load a Microplate onto the Plate Carrier press the Start button then switch 082 the Mains Power Supply to the unit when the Wash Head is between two Rows Verify that the gap between the delivery tubes in the head and the microplate being used is 1 0 1 5 If adjustment is necessary it can be achieved by slackening the lock nut beneath the dome nut on the Head Lift Rod adjusting the dome head nut and re tightening the lock nut 2 1 4 Pump Unit Filter Inspection Periodic inspection of the Pump Unit filter is recommended to ensure correct operation although replacement is seldom required inspect the filter remove the Pump Unit cover by taking out 2 fixing screws on the underneath 2 fixing screws at the front and 2 fixing screws at the rear of the unit Lift the cover clear of the chassis The filter is located at the front right hand corner of the chassis The filter cap can be removed without disconnection of the silicone tubing by firmly grasping the top of the filter and lifting in an upward direction Removal of the cap will expose the filter element which should be lifted out and checked for excess contamination If the element
59. the Pump from the Washer Unit Connect a Vacuum Flow Gauge to the vacuum pipe which would normally be connected to the Manifold Block on the Washer Unit Connect the Pump Unit to the appropriate mains supply and switch ON reading of gt 0 7 bar should be obtained If a reading outside this limit is obtained there is no adjustment available so a replacement Pump will be required 2 1 3 Wash Head Inspection To ensure even dispensing and washing it is neccessary that the Wash Heads and their end caps are kept clean and free of contamination and obstructions and that they are set to the correct height Some wash reagents contain buffer salts which can crystallise when the instrument is not in use this may cause clogging of of the wash aspirate tips To inspect the condition of the Wash Heads and their End Caps it is neccessary to remove the Head from the Washer Unit Lift the Head Assembly grasp the Wash Head centrally and slide it down in the direction of tips until it clears the End Caps 004 851 5 11 92 9 SENLEY INSTRUMENTS 170 SECTION 2 MAINTENANCE Using a De clogging Tool Part No 004 319 available from the Service Dept ensure all Dipense Tips are free from obstruction and clean as required Check the End Caps for signs of contamination or obstructions and inspect condition of O Ring seals clean and replace as required Refitting the Wash Head is a reversal of the removal procedure ensure
60. this has been found to be the optimum position 5 1 9 POSTWASH Delay time from Head down position to liquid dispense stop This switch controls the length of delay between when the Dispense Head is at the bottom of the plate well and the liquid stopping dispensing during the routines set on 61 TYPE and 52 TYPE switches The delay can be added incrementally in steps of 0 04 secs from a minimum setting of 0 27 secs to a maximum of 0 87 secs Setting the switch to position 5 gives 0 47 secs delay position 6 gives 0 51 secs delay position 7 gives 0 55 secs delay etc 5 1 10 8 12 WAY Switch This switch sets the washer to be used as either an 8 way 8 rows or a 12 way 12 rows washer With switch 1 set to ON and switch 2 set to OFF the unit will wash up to 8 rows with a 12 way wash head fitted With switch 1 set to OFF and switch 2 set to ON the unit will wash up to 12 rows wih an 8 way wash head fitted 004 851 5 11 92 DENLZY INSTRUMENTS 279 24 Document 004 006 100 X Release 3 0 WELLWASH 4 4Mk2 SERVICE MANUAL Spare parts Wellwash 4 AMk2 Page WELLWASH 4 2 SERVICE MANUAL Spare parts SUBJECT PAGE 1 onc Me T c 2 Main Darts M 3 Washer track 4 Control assembly H 6 Pump nit assembly uyu ag a
61. turning the switches through all of their positions from O to F at each position an LED should flash and the Audible Warning Device should Sound to confirm that the is satisfactory See the note at the end of this section for binary LED displays when performing the above and following checks 9 Press the Start key and repeat the above instructions for Sl REPT and S2 REPT switches 6 Press the Start key and repeat the above instructions for Sl PAUS and S2 PAUS switches 7 Press the Start key and repeat the above instructions for POSTWASH and PREWASH switches 8 Press the Start key then by pressing any of the Row Select keys 1 12 their corresponding LED s on the should illuminate and the AWD should sound 9 Press the Start key the setting of the DIL switch 081 will now be displayed by the LED s permanently LD3 and LD4 LD4 will remain permanently illuminated in this mode and LD3 will flash as switch 1 on 051 is switched On and Off 10 Pressing the Start key will effectively place the unit into a manual condition where the test engineer has total control over the instruments operation Pressing the Prime key checks that the Prime LED LD10 illuminates Row key 1 is inoperative Row key 2 checks the Audible Warning Device Row key 3 checks that the Vacuum Fail LED 1011 illuminates Rcw key 4 checks the correct operation of the Head Lift Motor and the Head Lift position sensor with Row B LED LD8 flashing and the A
62. two O rings in each end cap are in position and undamaged To install the alternative wash head slide it upwards onto the slots making sure that it is fully engaged Fig 5 7 Fig 5 15 Changing the wash head wash heads will only fit into the end slots when the raised flange 1 the wash head is facing the front Remember to check the program card accordingly 8 way or 12 way 5 4 Operational check The following procedure should be completed to confirm the correct functioning of the washer prior to normal use 1 Check that the wash head is correctly located by means of its guide slots in the head assembly Fig 5 7 The operational check is performed faster with the 12 way wash head 2 Add approximately 2 to 3 dl of distilled aqua to the wash bottle and make sure that both bottle caps are firmly tightened 3 Switch ON the mains power supply by depressing the POWER ON switch positioned on the rear panel of the pump unit The POWER indicator on the keypad Fig 4 2 will be illuminated and a countdown sequence will begin on the row indicators The row 12 indicator will illuminate first followed by row 11 row 10 row 9 and so on at one Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 24 Chapter 5 Installation second intervals until the PRIME indicator is reached The pressure generation takes 10 seconds The PRIME indicator continues flashing to prompt the user to prime th
63. unit conforms to the supply voltage used in the laboratory The instrument operates at voltages of 100 120 Vac 220 240 Vac and the frequency range 50 60 Hz see Section 5 3 step 2 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 16 Chapter 5 Installation Installation procedure Any suitably skilled person using standard tools may carry out the following procedure 1 Restthe pump unit on its side and remove the transit screws the Allen cap head type is painted red using the key supplied Fig 5 1 item 5 Relocate them in the retaining holes provided Fig 5 4 that the transit screws are refitted Failure to do so could invalidate the warranty Note When relocating this instrument or returning it for repair it is essential 2 Ensure that the pump mains switch is in the OFF position After checking that the electrical supply conforms to that specified on the voltage label on the rear panel of the unit slide the frequency selector switch in the pump model Cat no 5160771 to the 50 Hz or 60 Hz position depending on the mains supply voltage frequency Fig 5 4 Other pump models do not include a frequency selector FREQUENCY 50Hz eae gt t 55 ESE 60H gt SHIPMENT TRANSIT SCREWS MUST BE FITTEO IN THESE TWO POSITIONS PRIOR TO 1 Location of the frequency selector switch 2 Retaining holes for the transit screws 3
64. wool soaked in the 10 Formaldehyde solution into the bag Ensure the wad does not make contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hrs Remove the instrument from the bag Clean the instrument using a mild detergent Remove any stains using 70 alcohol Take the Plate Carriage and the Wash Head out of the gluteraldehyde solution and rinse with distilled water Reposition them on the unit Flush the fluid path of the instrument with distilled water using the priming procedure After performing this disinfection procedure label the instrument with a signed and dated decontamination certificate 5 HAZARDS This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to the user The following recommendations are given for added user safety 51 Liquid Reservoir IMPORTANT Due to the nature of the materials used for the manufacture of our reservoir bottles our suppliers have recommended that they are replaced after two years use This replacement policy must be more frequent if the bottles are stored or used in direct sunlight for long periods Labsystems are aware a potential leak in the pressure bottles when they have been subjected to long periods of UV radiation Although this occurance is very rare our customers are urged to follow this advice Bottles are available from Labsystems for b
65. 10 1 1990 A1 1992 A2 1995 including CENELEC Common Modifications US and CA National differences EN 61010 1 2001 Ed 2 9 3 In conformity with the requirements Wellwash 4 Mk 2 bears the following markings Type 006 100 120 Vac 200 240 Vac 50 60 Hz 300 VA CE mark Wellwash 4 Mk 2 conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B 2002 96 EC Waste of Electrical and Electronic Equipment EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 56 Chapter 10 Ordering Information 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Code 5160770 Instrument Wellwash 4 Mk 2 220 240 V 50 Hz 5160772 Wellwash 4 Mk 2 110 120 V AC 60 Hz 10 1 List of accessories Code Product N03283 2 wash bottle 1514313 2 waste bottle Wwwo04 1 12 way wash head WW2004 11 8 way wash head WW 004 48 Wash height adapter 10 2 List of recommended spare parts Code Product 00
66. 2 2 way pinch valve 000717019 3 Manifold assembly ooo appen cu 004 060L2 4 Solenoid grounding Wire 004 088L1 6 P nand End Cap ASSY uu aaa aa 004 077 7 R hand End 004 078 Document Release Page 004 006 100 X 3 0 5 ThermoFisher SCIENTIFIC WELLW ASH 4 2 SERVICE MANUAL CONTROL ASSEMBLY Page 6 Release 3 0 Document 004 006 100 X ThermoFisher SCIENTIFIC WELLWASH 4 4Mk2 SERVICE MANUAL Spare parts Control assembly POS ITEM CAT l Main plate assembly 230V Incl items 2 6 006 058AL2 Main plate assembly 115V Incl items 2 6 006 058BL2 2 Transformer 230V 004 075AL1 Transformer 115V T RR 004 075BL1 3 Fuse frame assembly P O 004 072L1 4 Mains filter assembly P O pe 004 073L1 5 Track motor assembly 230V 006 076AL1 Track motor assembly 115V 006 076BL1 6 Headlift motor assembly 230V P d 004 056AL1 Headlift motor assembly 115V P O 004 056 du Control peb 230V N03868 C
67. 6 612 006 613 006 613J 004 023D Spare parts bag for 220 V see Fig 5 1 Spare parts bag for 110 V Spare parts bag for 100 V Spare tubes bag see Fig 5 2 1210840 1210890 1210920 Spare fuses 0 5 A Spare fuses 1 A for 220 V Spare fuses 2 A for 100 V and 110 V Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 57 Chapter 11 Warranty Certificate 11 Warranty Certificate Thermo Fisher Scientific Microplate Instrumentation Business s Wellwash 4Mk2 is fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of twelve 12 months from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions Warranty is invalid if products have been misused or abused For this warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion
68. AYER CERAMIC 5 C23 TO C27 27 RESISTORS CARBO 28 000131104 56R 0 25W 3 R12 29 000131132 100R 0 25W 4 42223 58 30 000131148 120R 0 25W 20 R5 TO R10 R46 TO R55 R71 TO R74 31 000131160 180R 0 25W 1 R56 32 000131176 220R 0 25W 1 R62 33 000131211 470R 0 25W 3 R11 12 13 34 000131227 560R 0 25W 1 R15 35 000131255 1K 0 25W 7 R16 TO R21 R57 36 000131334 4K7 0 25W 3 R24 61 75 37 000131378 10K 0 25W 6 R25 26 R76 TO R79 38 000131457 47K 0 25W 2 R32 38 39 000131520 180K 0 25W 2 R33 34 40 000842769 82K 0 6W 1 R27 41 000842776 RESISTOR NETWORK 5X1K 1 RP4 42 000155352 CERAMIC W W RESISTOR 4 78 4W 1 R45 43 000305888 VOLTAGE REGULATOR uA 7805UC 1 44 000703015 VOLTAGE REG L78S12CV 12V 2 1 REG2 45 000283255 HIGH SPEED SUPPRESSOR 6 8V 2 01 2 46 000302198 QUARTZ CRYSTAL HC18U 6MHZ 1 XT 47 000785008 TRIAC MAC 97 6 3 SCR1 2 3 48 000842011 RESISTOR NETWORK 8x47K 5 1 2 3 5 6 49 000262113 RECTIFIER KBL 02 1 50 000711010 SLOTTED LTH 301 LITE 2 OSW1 2 Ls WHOLE OR PART MUST NOT 3 36336 6642 LEAN WITHOUT THE WRITTEN CONSENT 8 141255 5601 JK OF THE COMPANY 2 1996 155 DATE ECNNO CHK 155 DATE ECNNO Drawn Checked Approved Title CONTROL LOGIC Sheet Dept I A for Drg No D Napper J Hazell A Partridge PCB ASSEMBLY 12 WAY No 1 KEYPAD 110 120v 2 004 0668 51 000249794
69. CERAMIC 11 C7 TO C15 C20 C22 25 000702005 0 01 uF 400V POLYESTER 3 C17 TO C19 26 000702172 820 100v SINGLE LAYER CERAMIC 5 C23 TO C27 27 RESISTORSH 28 000131104 56R 0 25W 3 R1 2 3 29 000131132 1008 0 25W 4 R4 22 23 58 30 000131148 120R 0 25W 20 85 R10 R46 TO R55 a R71 TO R74 31 000131160 180R 0 25W 1 R56 32 000131176 220R 0 25W 1 R62 33 000131211 4708 0 2544 Oe 3 R11 12 13 34 000131227 560R 0 250 1 X I R15 35 000131255 1K 0 25W gt 7 R16 TO R21 R57 36 000131334 4 7 0 25W en 7 3 R24 61 75 37 000131378 10K 0 25W 6 R25 26 876 TO R79 28 000131457 47K 0 25W 2 R32 38 39 000131520 180K 0 25W 2 R33 34 40 000842769 82K 0 6W 1 R27 41 000842776 RESISTOR NETWORK 5X1K 1 RP4 42 000155352 CERAMIC W W RESISTOR 4 7R 4W 1 R45 43 000305888 VOLTAGE REGULATOR uA 7805UC 1 44 000703015 VOLTAGE REG 78512 12V 2 1 REG2 45 000283255 HIGH SPEED SUPPRESSOR 6 8V 2 012 46 000302198 QUARTZ CRYSTAL HC18U 6MHZ 1 XTi 47 000785008 TRIAC MAC 97 6 3 SCR1 2 3 48 000842011 RESISTOR NETWORK 8x47K 3 RP1 2 3 5 6 49 000262113 RECTIFIER KBL 02 1 50 000711010 SLOTTED LTH 301 LITE ON 2 OSW1 2 THIS ORAWING IS THE COPYRIGHT OF DENLEY INSTRUMENTS LTD COPIES IN WITHOUT THE WRITTEN CONSENT THE COMPANY 1996 Approved A Partridge Title CONTROL ASSEMBLY 12 WAY KEYPAD 220 240v VL for T 004 066 51 000249794 52 000848015 53
70. D J and C P Price 1997 Separation Techniques In Principles and Practice of Immunoassay 274 ed eds Price and Newman D J pp 154 172 Stockton Press New York Coupe and A M Cain Microplate washing the most important step in the ELISA process Labsystems Denley application note Beumer T Stoffelen E Smits J and Carpay W 1992 Microplate washing process description and improvements J Immunol Methods 154 77 87 Parry J V Mortimer P P Friederich P and Connell J A 1997 Faulty washers and soiled micropipettors may generate false positive serological results Clinical and Diagnostic Virology 7 173 181 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 63 Appendix Wellwash 4 2 Quick Reference Guide Fill the wash bottle and empty the waste bottle Plug the program card into the slot Position the plate carrier for priming Turn the instrument ON Place the microplate onto the plate carrier Remove the microplate from the plate carrier Turn the instrument OFF Thermo Scientific Wellwash 4 Mk 2 User Manual Appendix A Wellwash 4 Mk 2 Quick Reference Guide Ensure that the instrument is switched OFF Open the wash bottle cap Fig 6 1 very carefully to release any residual pressure Let the hissing sound end prior to opening the cap all the way Fill the wash bottle with approp
71. Dilute the cleaning agent as recommended by the manufacturer DO NOT expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Clean the keypad with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning Ifany surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 3 Warning If for any reason you need to move the instrument release first the pressure from the tubing by slightly opening the bottle cap Let the hissing sound end and then detach the yellow and blue coded tubes from the instrument 7 1 4 Immediate A 7 1 5 Tubing A Although the Wellwash 4 Mk 2 is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled aqua Spillages of liquid on the carrier slideway should be wiped off promptly as this may cause the plate carrier to drag during operation Caution DO NOT use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish Inspect the condition of the tubing regularly When you notice that a tube is becoming harder and it is no longer clear it should be replaced Only use tubing supplied by Thermo
72. Fisher Scientific Oy The instrument is supplied with spare tubes Fig 5 2 See Chapter 10 Ordering Information Warning Never detach a tube without first releasing the pressure by slightly opening the bottle cap Let the hissing sound end prior to detaching any of the tubes 7 1 6 Reagent waste bottles Due to the nature of the materials used for the manufacture of our reservoir bottles our suppliers have recommended that they be replaced after two years of use This replacement policy must be more frequent if the bottles are stored or used in direct sunlight for long periods of time Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 37 Chapter 7 Maintenance Thermo Fisher Scientific Oy is aware of a potential risk of leakage in the pressure bottles when they have been subjected to long periods of UV radiation Although this occurrence is very rare our customers are urged to follow this piece of advice Bottles are available from Thermo Fisher Scientific Oy for the Wellwash 4 Mk 2 Cat no WW934 1 Spare O rings for the bottle plugs are included in the spare parts bag delivered with the instrument see Fig 5 1 item 6 See Chapter 10 Ordering Information Ensure that the waste bottle is emptied regularly and at least every time the wash bottle is filled Do not let the liquid level exceed the level mark on the bottle The waste bottle is fitted with a liquid level sensor The sensor prevents the use o
73. In case of technical problems use the following trouble shooting list to diagnose the fault and restore the instrument to working order Problem Possible Cause Solution No POWER ON Mains lead not connected Connect lead to an indicator appropriate supply Fuse blown Replace fuse Pump Unit POWER switch Switch POWER switch ON is OFF VAC FAIL Air leaks Check Bottle Caps for leaks illuminated and tighten as required Constricted Tubing Check tubing for kinks Pump failure Check that the Pump Unit is switched ON Check that the Pump Unit is connected to the Washer Unit correctly Check Pump Unit fuse and replace if required Carrier does not move Program Card not inserted Insert Program Card when START is pressed Problem No delivery of Wash Reagent into wells Unequal dispensing or aspirating of Wash Reagent Over filling of wells during washing Possible Cause Silicon tube in pinch valve constantly pinched Air leaks at Reagent Bottle cap Tubing leaks Microplate incorrectly positioned on Plate Carrier Strips incorrectly installed in holder One or more dispense tips clogged Wash Head height requires adjustment Solution Pull tubing through and reform See 6 3 2 Tighten cap replace gasket if necessary Check tubing and tube connectors Check and adjust position of Plate Carrier Check that strips are correct and adjust as necessary De clog Washer Tip s See 6 3 3 Using spanners suppl
74. NUT M3 FULL HEX 4 55 000803010 NUT M4 FULL HEX 6 THIS DRAWING 15 THE COPYRIGHT OF DENLEY INSTRUMENTS LTD COPIES IN WHOLE OR PART MUST NOT MADE WITHOUT THE WRITTEN CONSENT 13 4196 5488 THE COMPANY 1995 Title VL for Org No CHASSIS ASSEMBLY PUMP UNIT 110 120V 60HZ 004 054B 12 3255015 ISONTT1I8 79 SNL 240238 577 3AON3H ss fers 56541 e 82d 21901 1OMINOO 031531 N338 SVH 824 WINN 122 HOlIDYdVO Lid LON 4 NO OVdA3M AVM ZL AT1B8W3SSV 9551 JO 1 25 02 LON ISNA luvd YO NOHA Ni 531402 ISNI JO 1HOhAgOO JHL SI ISM SW3ll 335 8 53903 4 6 5 AIdWOD OL 5498 TIY 3AOW3S ASV 18h00 NI dl X 1101 N39 G3393H9 335 U3ddvN d NAH S3NMOU S 3015 SIHL 3 dWiQ ZHO9 AOZL OLL INVIMVA ZHOS 0 2 072 LNVINWA D S NVINVA N SOd NOLBJ3SNI 3H0438 0737 619 40 50 31 H108 OL 38 OL 08 3A331S ONILVINSNI 9 SL ONISN 343415 38 24 30 58937 5 ZHO9 021 011 8
75. Push the de clogging tool into the thin metal tube Fig 7 7 De clogging the wash head Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 43 Chapter 7 Maintenance 7 1 10 Fuse replacement 1 Switch OFF the washer and disconnect the mains supply cable 2 Slide the washer and pump units open and disconnect the pressure and vacuum tubing from the washer unit pressure by slightly opening the wash bottle cap Let the hissing sound Warning Never disconnect the pressure tubing without first releasing the end prior to disconnecting the tubing Locate the fuse holders at the rear of the washer and pump units On the washer unit use a screwdriver to remove the fuse holder cap Turn the screwdriver counterclockwise about 45 to release it and then pull it out Fig 7 8 PRESSURE VACUUM 7 8 Opening the fuse holder of the washer unit 5 Remove the faulty fuse and discard it PRESSURE VACUUM PUMP CONTROL THIS EQUIPMENT BE CONI MUST VASA engen Fig 7 9 Washer unit fuse and fuse holder Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 44 Chapter 7 Maintenance 6 Ensure that the replacement has the same rating as appears on the rating label on the rear panel of the washer unit Only use fuses specified by Thermo Fisher Scientific Oy 7 Insert the new fuse to the fuse holder and push the holder into its place Turn the holder clockwise a
76. ROL LOGIC PCB ASSEMBLY 12 WAY KEYPAD 220 240v Sheet 22 2 a AWD1 RS 249 794 LD12 13 14 16 LD11 LD1 TO 2010 17 LD18 19 20 SW9 051 51 251 PL1 PLS T8124 TB3 EC1 FOR REG1 PL7 FOR 012 13 14 16 FOR SCR1 TO 3 FOR R45 C21 FIT TO C19 b pA 19 1996 FEEN E s d VL for Org No 004 066A H DE nn Billingshurst Sussex 14 9EY basis 1 004 801 1 2 004 325 LED SUPPORT 1 3 4 5 INTERGRATED CIRCUITS 6 000703065 MICRO PRO PD 8749 VERSION 4 1 7 000703017 74 LS 165 4 2 TO 05 8 000703018 74 LS 374 2 IC7 1C20 9 000703019 IC 74 LS 04 1 IC9 10 000703021 7 WAY DARL DRIVER ULN 2003N 1 11 000703022 TRIAC DRIVER 3041 3 11 12 13 12 000703023 7404 1 13 000702030 HALL EFFECT UGN 31200 1 14 14 000703066 IC 74 LS 125 1 22 15 000703067 74 LS 139 1 21 16 CAPACITORS 17 000101787 2 2 uF 35V SOLID TANTALUM 1 C3 18 000102702 10 uF 35V SOLID TANTALUM 1 C16 19 000104477 100 uF 16V ELECTROLITIC 1 C4 20 000103749 2200 uF 63V ELECTROLITIC 1 C61 21 000114424 0 47 uF 100V MIN LAYER 1 C6 22 000114418 0 22 uF 100V MIN LAYER 1 C5 23 000126124 22 pF 100V PLATE CERAMIC 2 C12 24 000702009 0 1 uF 25V DISC CERAMIC 11 C7 TO C15 C20 C22 25 000702005 0 01 uF 400V POLYESTER 3 C17 TO C19 26 000702172 820 uF 100v SINGLE L
77. Rev 2 3 Cat no 1507340 Thermo Scientific Wellwash 4 Mk 2 Cat 5160772 115 5160770 230 V or 5160771 100 V User Manual Rev 2 3 August 2007 Cat no 1507340 Copyright Copyright 2007 Thermo Fisher Scientific rights reserved Printed Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks and Wellwash is a registered trademark of Thermo Fisher Scientific other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions or for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Contact information Thermo Fisher Scientific Oy 100 01621 Vantaa Finland Tel 358 9 329 100 Fax 4 358 9 3291 0415 www thermo com Thermo Scientific Wellwash 4 Mk 2 User M
78. SERVICE MANUAL Wellwash 4 4Mk2 ThermoFisher SCIENTIFIC Thermo Fisher Scientific Ratastie 2 P O Box 100 01621 Vantaa Finland Telefax 358 9 32910 409 Tel 358 9 329 100 WW 004 853 17 10 2007 LABSYSTEMS ellwash 4 Operation manual LABSYSTEMS Information in this manual is subject to change without prior notice Cat no 1507330 The instrument has been designed and tested in accordance with DIN 57411 Pt 0411 Pt 1 and IEC Publication 348 Safety Requirements for Electronic Measuring Apparatus and has been supplied in safe condition The instrument fullfills the requirements related to the EU Directives 73 23 EEC related to safety Low Voltage Directive and 89 336 EEC as amended by 92 31 related to Electromagnetic Compatibility See section 1 3 for details The present instruction manual contains some information and markings which have to be followed by the user to ensure safe operation and to retain the instrument in a safe condition Every effort has been made to avoid errors in test and diagrams However Labsystems assumes no responsibility for any errors which may appear in this publication It is the policy of Labsystems to improve products as new techniques and components become available Labsystems therefore reserves the right to change specifications at any time We appreciate any comments on this publication Labsystems Wellwash 4 Operation Manual 10 7 1997 CONTENTS
79. SL V e DENLEY INSTRUMENTSLTO COPIEs IN iT ant WHOLE OR PART MUST NOT MADE ra pnus ot Pr cate ECNNO Che ATE ENG Gre WITHOUT THE WRITTEN CONSENT OF THE COMPANY 1996 Title CONTROL LOGIC UL for Org No PCB ASSEMBLY 12 WAY 004 066B Approved A Partridge KEYPAD 110 120v 4011 092 13104 0011 A389 UNOY 0HINOO PHA 9661 SL 30 DOENOO ANCHIS 300 38 JON Dd NO TOHA 3409 3339 unowo 5216192 201 AS OW lidSv ame i 69161 O ori Sol i 2 O 931 p ONISSOND 0432 1 sian E gt VO X VEQOZNIN 70574 BWOSE 1105 7105 824 AS el 91 M 019V N 2 9i 89I N 5 i 3053 8 33 HOSN3S YOSN3S 39Viuuvo L 7051 5 POL 63 05 35 Z 653 T jorami HOLIMS OVA
80. Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 67 Notes Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 68 Notes Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 69 Notes Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 70 WWO04 WELLWASH 4 MICROPLATE WASHER Natts Lane 0 d Billingshurst West Sussex S RH14 9EY 0 7 0403 783441 WW004 853 amp CONTENTS PAGE SECTION 1 THEORY OF OPERATION 2 1 1 General Description 3 1 2 Pump Unit Assembly 4 1 3 Washer Unit Assembly 5 1 4 Control Logic PAGE SECTION 2 MAINTENANCE 6 2 1 Preventative Maintenance 10 Zee Fault Finding 12 2 3 Removal Replacement Procedures 13 2 4 Calibration Test Procedures PAGE SECTION 3 PARTS LIST 19 Spare Parts 20 3 2 Tools Equimpent PAGE SECTION 4 DIAGRAMS 21 4 1 Diagrams PAGE SECTION 5 TECHNICAL BULLETINS 22 Sa Programmable Switch Card PAGE NOTES 25 004 851 5 11 92 DENLEY INSTRUMENTS L72 po SURO OF Oe The following paragraphs discuss the theory of operation of the Wellwash 4 Microplate Washer and sho
81. Transit screws during transportation Fig 5 4 Location of the frequency selector switch and the transit screws 3 Turn the pump unit back to its normal position Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 17 Chapter 5 Installation 4 Lift the wash arm assembly the stainless steel cover Remove the pinch valve holder Fig 5 5 before use Store the wire holder for future use __ 22 Pinch valve holder Fig 5 5 Pinch valve holder Note The wash heads are packed separately Install them before use 5 Install first the four 4 small O rings to the wash head holders two 2 on both sides Fig 5 6 Fig 5 6 Installing the rings to the wash head holders Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 18 Chapter 5 Installation 6 Slide the wash head between the holders Fig 5 7 Note The wash heads will only fit into the end slots when the raised flange on 1 the wash head is facing the front 5 7 Sliding the wash head between the holders 5 3 1 How to install the pump unit and washer unit connections Fig 5 8 Pump unit with tubes installed 1 The wash bottle with the black connections is located on the right hand side of the pump unit and the waste bottle to the left the tubing and connections are color coded to ensure correct connection Table 5 2 Table 5 2 Silicon tubing color codes Pressure feed from the pump to
82. WD sounding as the Hall effect magnet pesses the Hall Effect Device 14 to confirm correct Operation Row key 5 checks the forward drive of the Plate Indexing Mechanism and the correct operation of Indexing Sensor 0542 the opto comb passes through the Indexing Sensor 0592 both the Row 7 LED indicator 107 and the indicator LED LD13 will flash and the AWD will sound to confirm correct operation ONLY FOR OLD WW4 MODELS 004 851 5 11 92 9 INSTRUMENTS LTD 14 SECTION Row key 6 checks the return drive cf the Plate Indexing Mechanism and the correct operation of both the Indexing Sensor 0542 and the Home Sensor OSW1 As the Opto comb passes through the Indexing Sensor 0SW2 both the Row 7 LED 107 and the indicator LED 1014 will flash and the AWD will sound to confirm correct operation Returning the Indexing Mechanism to its Home position at the Home Sensor 0591 will cause the Row 6 LED to illuminate and the AWD to sound confirming correct operation Row key 7 checks operation of the Solenoid Valve an audible click will be heard from the Valve and the indicator LED LD16 will flash to confirm correct Operation Row 8 key will exit from the last test Row 9 key will begin the test cycle again starting with the Prime LED illumination check NOTE When Test Sequence has been completed 0 1 Switch 1 must be returned to the OFF position BINARY DISPLAY ON ROW SELECT BUTTONS 1 2 4 8
83. WN 310 28 29 000543901 CABLE TIE BASE 4 30 000543428 CABLE TIE 10 31 000892012 LABEL TRANSIT SCREW 1 32 33 000757031 BARBED CONNECTOR 1 34 35 000789008 FEET 4 36 000892019 LABEL TUV TEST MARK 1 37 000802449 SCREW M3 x 8 RSD CSK HD POZI 4 38 000801831 SCREW No6 x 3 8 PN HD S TAP POZ 3 39 40 41 42 43 44 45 000804076 WASHER M3 WAVE 7 46 47 48 000804078 WASHER M4 WAVE 4 THIS ORAWING IS THE COPYRIGHT OENLEY INSTRUMENTS LTD COPIES IN WHOLE OR PART MUST NOT 8E MADE WITHOUT THE WRITTEN CONSENT THE COMPANY 1995 HR L ww IIS ee eru Ie Sheet Dept CHASSIS ASSEMBLY 1 22 PUMP UNIT 220 240 50 2 of 2 for Org No 004 054A 51 000803008 NUT M3 FULL HEX 4 55 000803010 NUT M4 FULL HEX 6 Next Assembly 0041053 __ ____ __________________ THIS DRAWING IS THE COPYRIGHT OF LLL L L L L j OENLEY INSTRUMENTS LTD COPIESIN WHOLE OR PART MUST NOT BE MADE Hu ux tft WITHOUT THE WRITTEN CONSENT 12 44396 GOES OF THE COMPANY 1995 is oare EcNNO CHK SS DATE ECNNO Drawn Checked Approved Title Sheet for Org No CHASSIS ASSEMBLY PUMP UNIT 220 240V 50HZ D Napper D Roderick S Downes 004 054A Drawing _ Description _ E Billingshurst Sussex RH14 9EY England __9 _ Rema
84. Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 31 Thermo Scientific Wellwash 4 Mk 2 User Manual Chapter 6 Operation Contained on the programmable switch card are four 4 rotary type and nine 9 two way type switches each marked with their function The setting of these switches controls the function of the washer enabling a wide variety of protocols to be performed WW 006 10C1 ELECTRON CORPORATION SOAK X 0 5 MIN VOLUME X50 uL Fig 6 3 Programmable switch card Two way switch functions Thermo WELLWASH4 MK2 Switch settings SINGLE Make sure that this switch is always set to single wash head 8 WAY Use with an 8 way wash head PLATE All selected rows are washed once the instrument waits during the PAUSE setting and the following wash cycle is started 12 WAY Use with a 12 way wash head STRIP Each row is washed the set number of times WASHES The PAUSE setting is between each row STEPOFF The plate carrier will only pass over the selected rows With this selection the plate processing times are kept to a minimum STEPOVR The plate carrier will pass over all the rows not washing the rows not selected With this selection the time used for each plate remains constant WASHLO The wash head fills the well while rising from the bottom of the well WASHHI The wash head fills the well from the raised position WET The plate will re
85. ad this manual in its entirety The instrument should be unpacked and installed in accordance with Section 2 Particular attention should be paid to the correct fitting of the tubing to obviate leaks See Fig 2 Always ensure that the electrical supply in the laboratory conforms to that specified on the voltage label on the rear of the Pump Unit 4 2 Cleanliness Please clean the instrument regularly as stated below 4 2 1 4 2 2 4 3 It is recommended that the case of the instrument is cleaned periodically to maintain its good appearance It is particularly essential that the Carrier slideway be clean and dry to prevent jamming A soft cloth dampened in warm mild detergent solution will be found to suffice If any surfaces have been contaminated with biohazardous material a mild sterilising solution should be used In Operation Ensure that the Waste Bottle is emptied regularly and at least every time that a Reagent Bottle is loaded The Liquid Trap Bottle which prevents liquid from entering the Pump should be kept dry at all times Before pressing START button ensure 1 that the Wash Head is fitted 2 that the microplate is correctly orientated 3 that before any Program Card is fitted it should be wiped with a clean dry cloth to prevent any possibility of malfunctions 4 that the appropriate Program Card is fitted for procedure required 5 that if only part of a plate is to be washed the correct button is selected
86. age the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 15 Chapter 5 Installation 5 2 4 Environmental requirements When you set up your Wellwash 4 Mk 2 avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables wash bottles etc e Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make
87. alve and disconnect the tube from the Wash Head End Cap Replace the tubing and re assemble by reversing the above steps 004 851 5 11 92 9 DENLZY INSTRUMENTS LTD SECTION 2 2 1 2 Pump Unit Pressure and Vacuum Check To ensure correct volumes are dispensed it is neccessarv that the Pump Unit is set and maintained at the correct setting of 6 0 6 5 p s i 0 37 0 40 Bar To check the Pump Unit pressure setting empty the Wash Reagent Bottle and disconnect the Pump Unit from the Washer Unit Connect a Pressure Gauge 0 15 psi range to the fluid feed pipe which would normally be connected to the Manifold Block in the Washer Unit Connect the Unit to the appropriate mains supply and switch ON A reading of between 6 0 and 6 5 p s i should be obtained If a reading outside these limits is obtained adjustment must be performed To adjust the pressure locate the Pressure Adjustment Screw which is a chromed lock nut and screw located in a recess at the front of the pump above the Mains Outlet After releasing the lock nut the pressure may be adjusted by turning the screw turning anti clockwise will decrease the pressure turning clockwise will increase the pressure Once the pressure has been correctly set re tighten the lock nut To ensure that the unit aspirates fully it is important that the Pump Unit vacuum is functioning at the correct setting of 0 7 bar To check the Pump Unit vacuum disconnect
88. am e Excellent washing performance Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Chapter 3 Introduction to the Wellwash 4 Mk 2 Quickrelease wash waste bottle caps e Automatic pump shutdown e Effective liquid management Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 10 Chapter 4 Functional Description 4 Functional Description The Wellwash 4 Mk 2 is designed to wash wells in an 8 x 12 microplate It comprises a pump unit and a washer unit Fig 4 1 which are connected by power air and liquid lines The pump unit consists of a double headed vacuum pump with high density polythene reagent and waste bottles and a liquid trap The main power ON OFF switch is at the rear of the pump unit The frequency selector switch is located on the bottom side of the pump model Cat no 5190771 near the transport locking screws The keypad is located on the left hand side of the washer unit and the microprocessor and associated control electronics are housed under the keypad The wash head is located above the plate carrier slideway which can be either 8 or 12 way The plate carrier moves the microplate accurately beneath the wash head and incorporates a priming reservoir at its leading edge The plate carrier is indexed forward by means of a magnetic coupling to the drive system housed inside the washer 1 Pump unit 8 Wash arm assembly 2 Washer unit 9 Wash head and
89. am 004 752 Plumbing Diagram 004 752 Items List 1 Sheet 1 2 1 Pump Unit Description The Pump Unit Assembly has three main functions l To provide electrical power for the Washer Unit 2 TO provide a pressurised supply of wash fluid to the iWasher Unit 3 To provide vacuum supply and reservoir to the Washer Unit for the removal of waste fluid Housed within the unit are tbe following controls and components Main Power Inlet and Power Outlet for Washer Unit Power On Off Switch Mains Filter Double Ended Pressure Vacuum Diaphragm Pump and associated Filters Silencers and Tubing Waste Overflow Bottle Housed externally are the Fluid and the Waste Bottles and their associated Tubing Located in the case is the Pump which is a Dcuble Ended Diagphram type with both IN ports joined together to provide maximum vacuum A single OUT port is connected to the Fluid Bottle to provide the pressurised dispense the remaining OUT port is exhausted via the rear of the Unit 004 851 5 11 92 DENLEY INSTRUMENTS LTD RORY OPERATION 1 3 Washer Unit Assembly Diagrams Required 004 050 General Assembly 004 050 Items List 1 Sheet 004 051 Chassis Assembly 004 051 Items Lists 3 Sheets 004 052 Cover Assembly 004 052 Items List 1 Sheet 004 752 Plumbing Diagram 004 752 Items List 1 Sheet 004 750 Wiring Diagram 1 3 1 Washer Unit Description The Washer Unit Assembly has two main functions l To p
90. an inspection by the Carrier and notify your supplier in writing within three days Neither the Manufacturer nor its agents can be held responsible for damage in transit but will make every effort to help obtain restitution from the Carrier Upon receipt of the Carrier s inspection report arrangements will be made for repair or replacement 2 2 Site Requirements The Washer and Pump Units require no special site preparation A normal laboratory bench close to a mains supply socket is quite suitable Check that the voltage label on the rear of the Pump Unit conforms to the supply voltage in the laboratory Wellwash 4 does not produce operating noise at a level which could cause a hazard No sound level measurements are needed after installation 2 3 Installation Procedure The following procedure may be carried out by any suitably skilled person using standard tools 2 3 1 Rest the Pump Unit on its side to and remove the transit screws Allen cap head type painted red using the key supplied Relocate them in the retaining holes provided NOTE When moving this instrument to a different location or returning it for repair it is essential that the transit screws are refitted Failure to do so could invalidate the warranty 2 3 2 Remove the two black plastic caps from the hose connectors at the rear of the Washer Unit 2 3 3 wb Fig 2 Connect the pressure aspirate and vacuum tubing from the Pump Unit to the Washer Unit Al
91. anual Rev 2 3 Cat no 1507340 Table of Contents Table of Contents WO N 13 Appendix Appendix Appendix D Safety Symbols and 5 4 4 40000 7 About the User 2 ener nennen nennen 8 Introduction to the Wellwash 4 2 9 Functional 11 CIE 13 22222 26 2 cvwadec SNAKEN NNA AA KENAA a ENAME 36 Troubleshooting 49 Technical 55 Ordering Information 0 22412 57 Warranty Certificate 58 lie masa aun CE 59 Glossary and Abbreviations 63 Wellwash 4 Mk 2 Quick Reference 64 Certificate of Decontamination 1 11 65 Wellwash 4 Mk 2 Fe
92. ash am assembly tUbIng RE ead 15 Location of the frequency selector switch and the transit screws 17 Pinch valve holder 2 18 Installing the rings to the wash head holders 222 000 000000000000 18 Sliding the wash head between the holders Pump unit with tubes installed 2 Liquid level sensor system naa hA aiani plastic Cabs i ved eerte eee ntn Connection of pump and washer units Opening and closing the Correct position of the plate carrier E Loosening the locknut with Changing the wash head siu b dip Placing the microplate with 12 way 8 way wash head nu Releasing the pressure from the wash bottle 30 Preprogrammed three washes with 12 way wash 31 Programmable switch card Liquid level sensor and liquid trap bottle 38 Opening the liquid trap bottle 2 2 2 21 01 010111000000 39 Pulling the thick tube out of the liquid trap bottle 40 the thin tube out of the liquid trap bottle 2 40 Opening the liquid trap
93. bout 45 with the screwdriver 8 Onthe pump unit use the screwdriver to gently pry the fuse folder open THIS Equip BEC MENT M ONNECTED TOA PROS TH MAINS SUPPLY Fig 7 10 Opening the fuse holder of the pump unit 9 There are two fuses inside the holder The outer one is a spare fuse and the inner one is the one in use Lo x Mutt vO VYXO C lt MSS SUPPLY 14 57 POWER Fig 7 17 Pump unit fuse holder opened 10 Remove the inner fuse and discard it 11 Ensure that the replacement fuse have the same rating as appears on the rating label on the rear panel of the pump unit 12 Insert the new fuse the spare fuse to the inner slot of the fuse holder and push the holder into its place 13 Reconnect the tubing and the washer and pump units Reconnect the instrument to the mains supply If the fuses blow again contact authorized service See Section 8 2 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 45 Chapter 7 Maintenance Note Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders are prohibited 7 2 Disposal of materials When disposing of used material follow the Good Laboratory Practices Refer to local regulations for the disposal of infectious material Warning The sample
94. certified by the local authorities The mains plug should only be inserted into a socket outlet provided with a protective ground contact The protective action must not be negated by use of an extension cable without a protective ground wire Warning DO NOT replace fuses without first disconnecting the mains supply cable Only use fuses specified by Thermo Fisher Scientific Oy Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 52 Chapter 8 Troubleshooting Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders are prohibited covers or the removal of components is likely to expose live parts Before the instrument is opened for any adjustment replacement maintenance or repair disconnect it from all voltage sources by disconnecting the mains supply cable Warning When the instrument is connected to the mains supply the opening of Any adjustment maintenance or repair of the opened instrument under voltage should be avoided if possible but if unavoidable should be carried out only by a skilled technician aware of the hazard The same precautions applicable when using any electrical equipment should naturally be observed with this instrument DO NOT touch switches or electrical outlets with wet hands Switch the instrument OFF before disconnecting it from the mains supply 8 3 3 M
95. cted Fuse blown The pump unit POWER switch is OFF Connect the lead to an appropriate supply Replace the fuse Switch the POWER switch ON 2 VACFAIL illuminated Wash bottle is full Air leaks Constricted tubing Pump failure Empty the waste bottle Check the bottle caps for leaks and tighten as required Check the tubing for kinks Check that the pump unit is switched ON Check that the pump unit is correctly connected to the washer unit Check the pump unit fuse and replace it if required 3 Carrier does not move when START is pressed audible warning sounds 3 times Program card not inserted No row is selected Insert the program card 6 5 Select the row number of the last row to be washed 4 No delivery of wash reagent into wells Silicon tube in pinch valve constantly pinched Air leaks at the wash bottle cap Tubing leaks Push the pinch valve and pull the tubing through the valve and reform 7 1 5 Tighten cap replace gasket if necessary Check the tubing and tube connectors 5 Too small dispensing of wash reagent into wells 6 Unequal dispensing or aspirating of wash reagent Constricted or clogged tubing Microplate incorrectly positioned on the plate carrier Strips incorrectly installed in the holder One or more dispense tips clogged Check the tubing and tube connectors for kinks and obstacles Check and adjust th
96. cuum Pump with high density polythene Reagent and Waste Bottles and Liquid Trap At the rear of the Pump Unit is the main power on off switch Located on the left hand side of the Washer Unit unit is the Control Panel under which the microprocessor and associated control electronics are housed Above the Plate Carrier slideway is the Wash Head which can be either 8 or 12 way A Plate Carrier locates the microplate accurately beneath the Wash Head and incorporates a Priming Reservoir at its leading edge The carrier is indexed foward by means of a magnetic coupling to the drive system housed inside the Washer 1 Pump Unit 6 Plate carrier 2 Washer Unit 7 Wash head 3 Waste Bottle 8 Program card 4 Wash Bottle 9 Pinch valve 5 Control panel 10 Head assembly Fig 1 Wellwash 4 Microplate Washer 1 2 Safety and Power Requirements 1 2 1 International Safety Warning IEC 348 Class 1 This manual contains information and warnings which must be observed by the user to ensure safe operation and retain the instrument in a safe condition The instrument has been designed for indoor use within the specified limits of temperature Le 5 C to 40 RH 90 max and an altitude below 2000 m It should not be switched on if there are obvious signs of mechanical damage and it should not be used under wet conditions 1 2 2 Grounding Earth The instrument must be operated with a protective ground connected via the appropriate yellow green
97. d 7 1 4 Check the condition of the tubing and the wash and e waste bottles See Sections 7 1 5 and 7 1 6 Check the pinch valve See Section 7 1 8 Unclog the wash head tips See Section 7 1 9 Change the fuses when blown See Section 7 1 10 e Decontaminate the instrument if any biohazardous material has been spilled on it or when shipping it to service See Section 7 3 Perform the operational check regularly See Section 5 4 Arrange for the yearly servicing of the instrument Section 7 1 1 above 7 1 3 General Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied For reliable daily operation keep the instrument free of dust and spills from liquids It is also advisable to cover the instrument with the dust cover supplied when not in use In the event of any damage contact your local Thermo Fisher Scientific representative for service Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 36 A Chapter 7 Maintenance Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance soft cloth dampened in a warm mild detergent solution will be sufficient Caution Painted surfaces can be cleaned with most laboratory detergents
98. e position of the plate carrier Check that the strips are correct and adjust as necessary De clog the washer tip s 7 1 9 7 Overfilling of wells during washing 8 Leakage between wash cycles Wash head height requires adjustment Air leaks at the waste bottle cap Pinch valve not functioning properly By using the spanners supplied adjust the wash head height by loosening the locknut lowering the domed nut and locking into position on the head lift rod 5 3 2 Tighten cap replace gasket if necessary Push the pinch valve and pull the tubing through the valve and reform 7 1 5 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 50 Wash arm assembly not moving as it should Chapter 8 Troubleshooting Contact authorized service 10 Plate carrier not moving as it should Obstacle on the plate carrier slideway Keep the slideway clean 11 Wash cycle does not correspond to the program card Program card not inserted all the way Program card is dirty Program card is damaged Push the program card all the way into the slot Clean the program card Replace the program card Contact your local Thermo Fisher Scientific representative 12 Instrument not functioning as it should Press the RESET button If that doesn t help switch the instrument OFF and ON again If the problem persists contact authoriz
99. e appropriate programme card is to automatically control all aspects of the washing and aspirating of a microplate The PCB utilises a single chip microprocessor UPD 8749 which incorporates CPU RAM EPROM and Input Outputs The microprocessor and its associated support circuits are all contained on the PCB along with the control switches input sensors control outputs display devices and power supplies The following is a more detailed circuit description Read in conjunction with circuit diagram 004 751 is the microprocessor which has a built in xtal oscillator controlled by XT1 Inputs from the sensors enter the microprocessor via ports P22 to P28 Inputs from the sensors enter the microprocessor via ports P22 to P28 Inputs from the Carriage Home and Carriage Index sensors are buffered IC8 Pins 8 and 9 IC9 Pins 1 and 2 Start and Prime switches are connected directly to the microprocessor INT or interrupt output SWJ the Internal Reset Switch connects directly the microprocessor Reset Input along with the Power Up Reset components RP6 and C3 The Serial Input Port of the microprocessor TO takes its input from IC s 2 3 4 5 and the Serial Extension Connector PL6 The Serial Input IC s 2 3 4 and 5 are shift registers with parallel load inputs connected to the Programme Card Socket PL7 thus allowing the remote selection of Wash Programmes All of the Shift Registers are loaded by SH LD inputs being low and are
100. e of Decontamination see Appendix B as well as the return authorization number RGA are sent with the instrument Thermo Fisher Scientific s service department will keep you up to date with the progress of service and provide you with any further details you might need e g on maintenance serviceability troubleshooting and replacement 8 3 Limitations warnings and cautions This instrument is designed to provide full user protection When correctly installed operated and maintained it will present no hazard to the user The following recommendations are given for added user safety 8 3 1 Liquid reservoir IMPORTANT 8 3 2 Electrical A Due to the nature of the materials used for the manufacture of our reservoir bottles our suppliers have recommended that they are replaced after two years of use This replacement policy must be more frequent if the bottles are stored or used in direct sunlight for long periods of time Thermo Fisher Scientific Oy is aware of a potential leak in the pressure bottles when they have been subjected to long periods of UV radiation Although this occurrence is very rare our customers are urged to follow this piece of advice Bottles are available from Thermo Fisher Scientific Oy for the Wellwash 4 Mk 2 Cat no WW934 1 See Chapter 10 Ordering Information Ensure that the mains supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables
101. e plate carrier and the wash head out of the glutaraldehyde solution and rinse with distilled water Reposition them on the unit Flush the fluid path of the instrument with deionized distilled aqua using the priming procedure The wash heads can be sterilized in 7096 ethanol Also wash and decontaminate the wash rinse and waste bottles 7 4 How to pack for service When you ship the instrument for service remember to Inform about the use of hazardous materials Decontaminate the instrument beforehand Screw the transit screws into the bottom of the instrument to the correct shipment location Pack the instrument according to the enclosed packing instructions Section 7 4 1 Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges Enclose a dated and signed Certificate of Decontamination Appendix B both inside and attached to the outside of the package in which you return your instrument or other items Enclose the return authorization number RGA given by your Thermo Fisher Scientific representative Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s service department Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 47 Chapter 7 Maintenance See Section 9 1 for details on storage and transportation temperatures See
102. e system 4 Select the preprogrammed wash card for 1 wash and for the 12 way wash head Plug it into the slot on the right hand side of the washer unit Fig 4 1 item 13 5 Liftthe wash head and slide the plate carrier onto the stainless steel slideway so that the reservoir is under the wash head Fig 5 13 6 The instrument should now be primed Press the PRIME button to initiate the sequence The wash head will lift and the pinch valve will open for one second allowing wash fluid to flow into the priming reservoir The wash head will then lower itself to empty the prime reservoir The PRIME indicator will turn off when the priming is complete If no further key entry is made within ten 10 seconds of priming the pump will automatically switch OFF The instrument will then enter its idle state waiting for instructions from the keypad and program card 7 Place a microplate onto the plate carrier When using a 12 way wash head the well A1 of the microplate should be positioned in the top left hand corner When using an 8 way wash head the well A1 should be positioned in the top right hand corner See Fig 5 16 Fig 5 16 Placing the microplate with 12 way and 8 way wash head 8 Select the row number of the last row to be washed on the keypad e g when button 4 is pressed rows 1 to 4 will be washed Note When the 12 way program card is installed buttons 9 to 12 are 1 inoperative 9 Press the START button to set th
103. e washing cycle into operation The plate carrier will be drawn into the washer filling and aspirating each row according to the sequence selected on the program card At the end of a sequence rows will be left substantially dry and the plate carrier will stop with the priming reservoir under the wash head 10 The instrument is performing properly if the wells are dry If they are not check that both bottle caps are firmly closed Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 25 Chapter 6 Operation 6 1 Operational checklist Table 6 7 Operational checklist Tick Item Fill the wash bottle with appropriate washing reagent Select the program card and insert it When using the programmable switch card check the settings Switch the instrument ON Prime the instrument Place a microplate onto the plate carrier Select the row number of the last row to be washed Start the wash sequence When the wash sequence has finished remove the microplate D DL L D D D L UD LD Shut down the instrument The following symbols are used in the flowchart below Press the key Perform Indicates the actions you should take action Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 26 Chapter 6 Operation 6 2 Washing a microplate Fill the wash bottle and empty the waste bottle Plug the program card into the slot Position the plate carr
104. ead Press the PRIME button The Wash Head will operate up and down aspirating and dispensing into the Reservoir finally stopping in the down position The priming function is complete when PRIME indicator is permanently illuminated NOTE priming procedure must be repeated whenever the POWER switch has been operated or there has been an interruption of the power supply Loading a Microplate Place the microplate on the Plate Carrier ensuring that it is correctly orientated See Section 2 4 6 When using removable well plates ensure that no wells are missing from rows to be washed and that all wells are level on the Plate Wellwash 4 has been designed to work with the majority of plates in common use If you experience difficulty with the location of a particular microplate please contact the Manufacturer or his approved distributor Examples of Wash Routines Wellwash 4 is programmable by use of simple plug in Program Cards for each assay or routine required Functions preset or which can be included in any routine are as follows Aspirate and fill rows sequentially once only with a final aspirate Aspirate and fill rows 3 times followed by a final aspirate 2 3 Aspirate and fill rows 4 times with a final aspirate 4 Aspirate fill aspirate rows once sequentially followed by aspirate and fill rows 3 times with a final aspirate 5 Aspirate and fill rows 4 times with 3 seconds pause after each row followed by a final aspira
105. echanical and environmental Mechanical The wash head is a free moving mechanical device controlled by the instrument and not directly by the operator It is designed for hands off operation and should be used as such Never reach into the workspace while the instrument is performing a wash cycle If it is necessary to stop the operation of the equipment press the RESET button on the keypad Never open the wash bottle detach any of the tubing or press the black top of the pinch valve without first releasing the pressure by slightly opening the bottle cap Let the hissing sound end prior to proceeding Environmental Infectious clinical samples and corrosive fluids are commonly used with this equipment The hands off nature of the system allows the user to wash the reaction wells without getting into direct contact with these fluids However wells that have been in contact with potentially hazardous fluids must be handled before and after the wash process and this should be done with utmost care Hand and eye protections should always be worn as well as corrosive resistant laboratory coats Q If you are using a washing reagent that is either volatile or easily splashing or gt P when you are washing microplates with samples of human or animal origin keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into a fume hood When spills get into contact with the carrier sl
106. ed service DO NOT use the instrument if it appears that it does not function properly If you were able to correct the error without having to turn off the instrument you may continue instrument operation by pressing start Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 51 Chapter 8 Troubleshooting 8 2 Service request protocol If the Wellwash 4 Mk 2 requires service contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s service department DO NOT under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your Thermo Fisher Scientific representative or distributor will take care of sending a complaint form Complaint order to Thermo Fisher Scientific s service department The Complaint order contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill in and forward the Complaint order to Thermo Fisher Scientific s service department Check Section 7 4 How to pack for service You will find instructions on how to proceed before shipping the instrument for service Check that any necessary decontamination procedure has been carried out before packing See Section 7 3 Decontamination procedure Ensure that the Certificat
107. edback Form 66 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Table of Contents Table of Contents 1 2 3 3 1 3 2 3 3 4 4 1 5 5 1 5 2 5 2 1 5 2 2 5 2 3 5 2 4 5 2 5 5 2 6 5 3 5 3 1 5 3 2 5 3 3 5 3 4 5 4 6 1 6 2 6 3 6 4 6 5 6 5 1 6 5 2 6 6 6 7 6 7 1 6 7 2 6 8 7 1 7 1 1 7 1 2 7 1 3 7 1 4 7 1 5 7 1 6 7 1 7 Safety Symbols and 5 4 4 40000 7 About the User Manual cerent 8 Introduction to the Wellwash 4 2 9 Intended Use IRE NEM 9 Principle of nee ei tre eter been eoe Cbr ka Ro PETERE ned MEC RARE nt Re 9 Advantages of using Wellwash 4 2 9 Functional Description nine dp Ree 11 Controls and iridiGators ERR REN Rc Ra ne 12 Installation su riim drain eerta 13 Installation check list u En a e Er 13 What do upon iii aiai aiaa ndani eaii 13 Howto unpack ioi 13 Checking delivery for completeness 1 6 enne 14 Checking for damage during 15 Environmental requirements 2 1 010
108. elivery for completeness Refer to 5 2 2 Check for damage during transport Refer to 5 2 3 Place the instrument on a normal laboratory bench taking into account both the environmental and technical prerequisites Refer to 5 2 4 and 5 2 6 Leave sufficient clearance on both sides and at the rear of the unit Install the instrument Remove the transit screws Refer to 5 3 step 1 Check the voltage and frequency of the electrical supply Refer to 5 3 step 2 Remove the pinch valve holder Refer to 5 3 step 4 Install the wash head Refer to 5 3 steps 5 and 6 Connect the tubing and the liquid level sensor cord to the pump unit Refer to 5 3 1 steps 1 to 5 Connect the washer unit to the pump unit Refer to 5 3 1 steps 6 and 7 Fill the wash bottle and tighten both bottle caps Refer to 5 3 1 steps 8 and 9 Slide the plate carrier to its place Refer to 5 3 1 step 10 Connect the mains supply cable cord to the mains input socket Refer to 5 3 2 Carry out the operational check Refer to 5 4 5 2 What to do upon delivery 5 2 1 How to unpack Move the unpacked instrument to its site of operation Unpack the Wellwash 4 Mk 2 instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package Caution the Warranty Certificate card the packing instructions packing
109. ermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 20 Chapter 5 Installation 6 Remove the three 3 black plastic caps from the hose connectors and the pump control at the rear of the washer unit Fig 5 10 Fig 5 10 Removing the plastic caps 7 Slide the two units together until the locating dowel and electrical connectors are firmly engaged Fig 5 11 Silicone tube waste blue color code Manifold pipes PRESSURE for reagent tube yellow VACUUM for waste tube blue Locating pin a and friction bush b Silicone tube wash yellow color code Signal connectors with text PUMP CONTROL Silicone tube pressure black color code Pump pressure adjustment screw do not touch for authorized service personnel only Fig 5 11 Connection of pump and washer units Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 21 Chapter 5 Installation 8 Open the wash bottle Fig 4 1 item 4 by rotating the cap ring while the white plug remains stationary Lift the cap ring and open the plug Fill the wash bottle up to the shoulder with wash reagent and reconnect ensuring that the plug mates correctly with the bottle insert Fig 5 12 and that the cap ring is tightly screwed down Fig 5 12 Opening and closing the bottle 9 Tighten the cap on the waste bottle 10 Lift the wash head and slide the plate carrier onto the stainless steel
110. ervoir The priming function is complete when the PRIME indicator turns OFF If no further key entry is made within ten seconds of priming the pump will automatically switch OFF The instrument will then enter the idle state waiting for instructions from the keypad and program card If the pump fails to start or if the bottle caps are loose an audible warning will be heard and the VAC FAIL indicator will illuminate This condition will clear once the pump has started If the VAC FAIL indicator does not clear after the pump has started the RESET key must be depressed Check also that the waste bottle is not full Note The priming procedure must be repeated whenever the POWER switch has been operated or if there has been an interruption of the power supply Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 29 Chapter 6 Operation 6 4 Adding wash reagent To add wash reagent first release the pressure from the wash bottle by carefully opening the bottle cap see Fig 6 1 Let the hissing sound end prior to opening the cap all the way 6 1 Releasing the pressure from the wash bottle Also when you are changing the used reagent and you wish to rinse and clean the tubing first release the pressure from the tubing and the wash bottle by slightly opening the wash bottle cap Close the wash bottle tightly Fig 5 12 The waste bottle needs to be emptied when the wash bottle is filled Thermo Sc
111. f the instrument after the waste bottle is filled to the mark 7 1 7 Liquid trap bottle The liquid trap bottle prevents liquid from entering the pump and it should be kept dry at all times Liquid should enter the liquid trap bottle only if the liquid level sensor is not properly connected Fig 7 1 item 1 and the waste bottle overflows If that happens follow immediately the steps below 1 Switch OFF the instrument and make sure there is no pressure in the wash bottle by opening the wash bottle cap slightly 2 Open the black screw on the cap of the liquid trap bottle cover Fig 7 1 item 2 EMPTIED WHEN FLUE REACHES THIS LEVE 1 Liquid level sensor 2 Liquid trap bottle cover screw Fig 7 1 Liquid level sensor and liquid trap bottle cover Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 38 Chapter 7 Maintenance 3 Release the cover by slightly lifting the tube and slide the cover then upwards along the tube Fig 7 2 Fig 7 2 Opening the liquid trap bottle cover Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 39 Chapter 7 Maintenance 4 Pull the thick vacuum tube with the 90 angle out of the liquid trap bottle 7 3 Pulling the thick tube out of the liquid trap bottle 5 Pull the thin tube out of the liquid trap bottle 7 4 Pulling the thin tube out of the liquid trap bottle Thermo Scientific Wellwash 4 Mk 2 User Manual
112. he Washer to aspirate the whole microplate Resetting Pressing the RESET button on the Control Panel will at any stage stop the immediate washing sequence and reset the system to its preprogrammed state Reagent Waste Bottles Ensure that the Waste Bottle is emptied regularly and at least every time a Reagent Bottle is loaded into the Washer The Liquid Trap Bottle which prevents liquid entering the Pump should be kept dry at all times Shut Down Procedure Wash Reagents may contain buffer salts which can crystallise when the Washer is not in use This may cause clogging of the wash aspirate tips To avoid this the following procedures should be followed 3 3 1 Daily Shut Down Procedure When the last washing cycle of the day is complete switch the power OFF through the switch at the rear of the Pump Unit Fill the Priming Reservoir of the Plate Carrier with distilled water and lower the Wash Head Tips into the Priming Reservoir Long Period Shut Down If the Washer is not likely to be used for a long period the system should be flushed through with distilled water prior to carrying out the DAILY shut down procedure Empty and clean the Waste Bottle and check that the Liquid Trap is dry When the Washer is to be used after prolonged shut down the system should be primed see Section 3 2 2 at least 4 times prior to carrying out any wash function 12 4 41 OPERATING PRECAUTIONS AND LIMITATIONS Before Operation Re
113. ideway it should be decontaminated with a hard surface disinfectant see Section 7 3 Observe Good Laboratory Practices GLP when handling potentially hazardous samples Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 53 Chapter 8 Troubleshooting 8 4 Defects and abnormal stresses Whenever it is likely that the protection has been impaired the instrument should be made inoperative and be secured against any unintended operation The protection is likely to be impaired if for example the instrument 1 Shows any visible damage 2 Fails to perform the intended functions 3 Has been subjected to prolonged storage under unfavorable conditions 4 Has been subjected to severe transport stresses 8 5 Before operation Read this manual in its entirety as it contains information necessary to ensure safe operation 8 5 1 Installation The instrument should be unpacked and installed in accordance with Chapter 5 Particular attention should be paid to the correct fitting of the tubing to obviate leaks Fig 5 11 Always ensure that the electrical supply in the laboratory conforms to that specified on the voltage label on the rear panel of the pump unit 8 5 2 operation Ensure that the waste bottle is emptied regularly and at least every time that a wash bottle is loaded The liquid trap bottle that prevents liquid from entering the pump should be kept dry at all times 8 6 Cleanliness Clean the
114. ied adjust the Wash Head height by loosening the lock nut lowering the domed nut and locking in position on the Head Lift Rod See 4 3 3 6 5 Fuse Replacement Switch off the Washer and disconnect the mains supply cord Locate the fuse holder at the rear of the Wash and Pump Units Using a coin or similar tool remove the fuse holder cap Remove the faulty fuse and discard Ensure that the replacement has the same rating as appears on the rating label on the rear of the Pump Unit Reconnect the Washer to the mains supply If the fuse blows again contact the authorised service See section 6 6 below NOTE Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuseholders is prohibited 6 6 Service Requests Should this instrument require service attention or if replacement parts are needed please contact Tel 358 9 329100 Fax 358 9 32910409 Labsystems Oy Technical Services Sorvaajankatu 15 00810 HELSINKI FINLAND or the local authorized Labsystems Distributor or Service agent 20 lt 5 gt Labsystems Manufacturer s Name Labsystems Oy Research Systems Division Manufacturer s Address Sorvaajankatu 15 P O Box 8 FIN 00881 Helsinki FINLAND Tel 358 9 329100 Telefax 358 9 32910415 Distributed by 21 Wellwash Thermo Scientific Wellwash 4 Mk 2
115. ientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 30 Chapter 6 Operation 6 5 Program selection Choose an appropriate card for the wash routine Plug the card into the slot located at the base to the right of the washer unit Note Before inserting any program card ensure that it is clean and dry Wiping the surfaces with a soft dry cloth will suffice If any liquid is present on the card a malfunction of the program may occur Note 1 If a run is started without a program card or any row selection the instrument will signal an error by sounding the audible warning device 3 times Note 2 If a run is started with the rows selected but with no program card m inserted the instrument aspirates the rows selected and returns to its idle state with the wash head lowered into the priming reservoir Note 3 If a run is started without any rows selected but with a program card fitted the instrument will aspirate all the rows in accordance with the card configuration i e 8 way or 12 way wash head m The preprogrammed wash cards will dispense and aspirate the plate row by row for the number of times indicated on the card There are four cards which can be inserted either side up to perform the wash routine indicated on the side facing up Thermo WELLWASH4 MK2 ELECTRON CORPORATION THIS WAY UP WW006 13C1 Lh dk a dhh Fig 6 2 Preprogrammed card three washes with 8 way wash head Thermo Scientific
116. ier for priming Turn the instrument ON Place the microplate onto the plate carrier Remove the microplate from the plate carrier Turn the instrument OFF Thermo Scientific Wellwash 4 Mk 2 User Manual Ensure that the instrument is switched OFF Open the wash bottle cap Fig 6 1 very carefully to release any residual pressure Let the hissing sound end prior to opening the cap all the way Fill the wash bottle with appropriate washing reagent and tighten the bottle cap firmly see Fig 5 12 Select either one of the preprogrammed wash cards or the programmable switch card see Section 6 5 Before inserting any program card ensure that it is clean and dry Wiping the surfaces with a soft dry cloth will suffice If any liquid is present on the card a malfunction of the program may occur Position the plate carrier on its slideway with the priming reservoir to the rear under the wash head see Fig 5 13 The POWER indicator on the keypad Fig 4 2 illuminates and a countdown sequence will begin on the row indicator LEDs The PRIME indicator flashes until you prime the system Section 6 3 The priming function is complete when the PRIME indicator turns OFF If no further key entry is made within fifteen seconds of priming then the pump will automatically switch OFF The instrument will then enter the idle state waiting for instructions from the keypad and program card Check that the cor
117. ing delivery for completeness Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy The instrument is supplied with both 8 and 12 way wash heads four program cards the user manual the mains supply cable and an accessory pack The content of the accessory pack is shown in Fig 5 1 and Fig 5 2 Store the accessories in their bags until you need them B H Combiplate 4 spare fuses see Section 7 1 10 Fuse replacement 2 Allen keys see Section 5 3 Installation procedure step 1 2 spanners see Section 5 3 2 Wash head height De clogging tool see Section 7 1 9 Unclogging tips 6 spare O rings for the bottle caps 4 spare O rings see Section 5 3 Installation procedure step 5 gt O Fig 5 1 Accessories included with instrument Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 14 Chapter 5 Installation Waste liquid spare tube Reagent liquid spare tube Pinch valve spare tubes item 3 in Fig 5 3 see Section 7 1 8 Pinch valve Wash arm assembly spare tube item 2 in Fig 5 3 Wash arm assembly spare tube item 4 in Fig 5 3 Wash arm assembly spare tube item 1 in Fig 5 3 DQ URUN Fig 5 2 Spare tubing Fig 5 3 Wash arm assembly tubing 5 2 3 Checking for damage during transport Visually inspect the transport pack
118. instrument regularly as stated below 1 It is recommended that the case of the instrument is cleaned periodically to maintain its good appearance It is particularly essential that the carrier slideway be clean and dry to prevent jamming A soft cloth dampened in warm mild detergent solution will suffice 2 surfaces have been contaminated with biohazardous material a mild N sterilizing solution should be used See Section 7 3 Decontamination procedure Caution DO NOT use any solutions containing hypochlorite such as bleach on any of the stainless steel surfaces as this may cause permanent damage to the finish Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 54 Chapter 9 Technical Specifications 9 Technical Specifications 9 1 General specifications General specifications Overall dimensions Washer unit 41 cm x 22 cm x 12 cm 16 1 x 8 7 x 4 771 Pump unit 30 cm x 18 cm x 16 cm 11 8 x 7 1 x 6 3 Weight Mains power supply Washer unit 4 5 kg 10 lbs 220 240 Vac 50 Hz 110 120 Vac 60 Hz 100 Vac 50 60 Hz Total 10 kg 22 Ibs Pump unit 5 5 kg 12 1 5 Power consumption 300 VA Heat dissipation 1024 BTU Operating cycle Continuous Fuse requirements Washer unit Pump unit Operating conditions Temperature Relative humidity Pressure 220 240 Vac 50 Hz 2 x 0 5 A 250 V UL 198G Time Delay 2 x 1 0 A 250 V UL 198G Time
119. ion Never open the wash bottle detach any of the tubing or press the black top of the pinch valve without first releasing the pressure by slightly opening the bottle cap Let the hissing sound end prior to opening the cap all the way If you are using a washing reagent that is either volatile or splashes easily keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into a fume hood When washing microplates with samples of human or animal origin keep yourself at a safe distance from the instrument while it is operating to avoid exposure Place the instrument into a fume hood If the pump fails to start or if the bottle caps are loose an audible warning will be heard and the VAC FAIL indicator will illuminate This condition will clear once the pump has started If the VAC FAIL indicator does not clear after the pump has started the RESET key must be depressed Also check that the waste bottle is not full The priming procedure must be repeated whenever the POWER switch has been engaged or if there has been an interruption of the power supply Make sure that sufficient reagent is present in the wash bottle to complete the required protocol See Fluid consumption in Section 9 1 General specifications If you use the programmable switch card ensure that the 8 WAY 12 WAY switch is in the correct position for either the 8 way or the 12 way wash head If removable well
120. l Panel and when illuminated indicates that the POWER switch is ON PRIME Push Button A momentary action switch which starts the PRIME function PRIME Indicator This yellow LED located to the right of the PRIME button flashes to indicate that the PRIME function is required and when permanently ON indicates that the Washer is primed and ready for use ROW SELECT 1 12 Push Buttons These are momentary action switches which select the last row to be washed in any chosen program sequence ROW SELECT Indicators These yellow LEDs located to the right of the ROW SELECT push buttons when illuminated indicate the rows selected for washing START Push Button This momentary action switch initiates the wash cycle RESET Push Button When depressed will reset the Washer to its preprogrammed state VACUUM FAIL Indicator A yellow LED which when flashing indicates a vacuum failure NOTE Operation of all buttons is confirmed by an audible tone from the Washer 10 3 2 3 2 1 3 2 2 3 2 3 3 2 4 3 2 5 3 2 6 Operating Procedures Switching ON Ensure that the switch located at the rear of the Pump Unit is in the OFF position Switch ON the mains supply and depress the POWER switch to ON The POWER ON indicator on the Control Panel should be illuminated and adjacent PRIME indicator should flash Priming Procedure Position the Plate Carrier on its slideway with the Priming Reservoir to the rear under the Wash H
121. l TYPE routine has been performed a second different routine s required The S2 set o switches should only be used when a second wash routine is needed for example when Aspirate Fill Aspirate routine is required to be followed by an Aspirate Fill routine this would allow the user to get rid of any high concentration waste prior to washing with a built in soak time Sw tch settings and corresponding routines are similiar to S1 TYPE Setting Routine 0 No Routine 1 Aspirate Only 2 Aspirate Fill move to next Row 3 Asoirate Fill Aspirate move to next Row 4 to F No Routine C3 Sl REPT Sequence 1 Set repeats per Row or Tray This switch sets the number of repeats of the routine set the 51 TYPE switch On this switch setting 0 will in fact give 1 repeat routine setting 1 will give 2 repeat routines 2 will give 3 repeat routines etc Note With Sl TYPE switch set in the Aspirate Fill routine the number of repeat routines set is the number of repeat routines per TRAY With 51 TYPE switch set in the Aspirate Fill Aspirate routine the number of repeat routines set is the number Of repeat routines per ROW 204 851 5 11 92 SENLEY INSTRUMENTS 172 22 SECTION 5 TECHNICAL BULLETINS 5 1 4 52 REPT Sequence 2 repeats per Row or Tray This switch sets the number o repeats of the routine set on the S2 TYPE switch in exactly the same manner as the Sl REPT switch re
122. l tubing and connections are colour coded to ensure correct connection Colour coding as follows Black Pressure feed from the Pump to the Wash Reagent Bottle Yellow Pressuring Wash Reagent from the Wash Reagent Bottle to the Washer Unit Red Vacuum feed from the Pump to the Waste Bottle Blue Waste feed from the Washer Unit to the Waste Bottle Connect the Vac Fail tubing from the front of the Pump Unit to the connector on the rear of the Washer Unit See Fig 2 Slide the two units together until the locating pegs are firmly engaged Ensure that the vacuum tubing is not kinked or pinched See Fig 2 Remove the Reagent Bottle Figure 1 3 1 4 by rotating it whilst holding the cap stationary Fill the Reagent Bottle up to the shoulder with wash reagent and reconnect ensuring that the pointed end of the wash reagent tubing is in contact with the bottom of the bottle and that the cap is tightly screwed down Tighten the cap on the Waste Bottle Place the Plate Carrier on the slideway orientated such that the Priming Reservoir is beneath the Wash Head Manifold pipes Locating pin Friction bush Silicone tube waste Silicone tube wash Connection of Pump and Washer Units Ensure that the Pump mains switch is in the OFF position After checking that the electrical supply conforms with that specified on the voltage label on the rear of the Unit plug the mains supply cable into the socket provided at the rear of the washer the co
123. lways use disposable gloves and protective clothing and operate in a well ventilated area 7 3 1 How to decontaminate the instrument 1 Prepare the decontaminant e g 200 ml 1096 formaldehyde solution 1 396 Virkon solution or 200 ml 496 glutaraldehyde solution or another agent recommended by your safety officer Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 46 10 11 12 13 14 15 16 17 18 Chapter 7 Maintenance Prime the unit with distilled water from the wash bottle Lift the wash head and fill the prime tray with glutaraldehyde solution Aspirate the prime tray using the wash head and then reprime the prime tray with distilled water Remove the plate carrier and the wash head and place into a glutaraldehyde solution for 24 hours Empty the fluid containers Switch OFF the power and disconnect the mains supply cable Decontaminate the outside of the unit using a wad of cotton wool soaked in 70 alcohol Place the entire instrument into a large plastic bag Ensure that the reagent containers are disconnected and open Place a wad of cotton wool soaked in 1096 formaldehyde solution into the bag Ensure that the wad does not make contact with the instrument Close the bag firmly and leave the instrument in the bag for at least 24 hours Remove the instrument from the bag Clean the instrument using a mild detergent Remove any stains using 7096 alcohol Take th
124. main filled after washing Use this setting with soak period DRY The plate will be aspirated after washing F WET The plate will remain filled after the final wash F DRY The plate will be aspirated after the final wash Rev 2 3 Cat no 1507340 32 Chapter 6 Operation F1 OFF FINAL X1 and F2 OFF FINAL X2 Table 6 2 Number of washes after a soak period Settings Number of washes F1 OFF 22 0 FINAL X1 F2 OFF 1 1 FINAL X2 2 FINAL X1 FINAL X2 3 PLATE STRIP and WET DRY Table 6 3 Aspiration after the final wash Settings PLATE WET No aspiration wells filled PLATE DRY All rows aspirated after the final wash STRIP WET No aspiration wells filled STRIP DRY Each row aspirated after its final wash SOAK 6 5 2 Rotary switch functions Set the soak period Table 6 4 which commences after all the presoak wash routines WASHES are complete Make sure you have selected WET Table 6 4 Soak period in minutes Setting 1 2 3 Soak time minutes 0 5 1 0 1 5 2 0 2 5 3 0 3 5 Setting F Soak time minutes 40 45 50 60 70 80 9 0 10 0 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 33 Chapter 6 Operation PAUSE Set the pause between each wash cycle Table 6 5 During the strip wash mode the pause is between each row During the plate wash mode the pause is between each pass
125. ment for various assemblies WARNING VOLTAGES DANGEROUS TO HUMAN LIFE ARE PRESENT IN THIS INSTRUMENT BEFORE REMOVING ANY COVERS DISCONNECT THE INSTRUMENT FROM THE MAINS SUPPLY 2 3 1 Diaphragm Pump Unit l Un d Remove the mains cable connector from the rear of the pump unit and disconnect the washer unit Unscrew the Wash Reagent and the Waste Bottle caps and remove the bottles from their retaining straps Using a suitable screwdriver remove the Pump Unit Cover by taking out 2 fixing screws on the underneath 2 fixing Screws at the front and 2 fixing screws at the rear of the unit Lift the cover clear of the chassis some of the tubing in the unit will have to be disconnected Disconnect all tubing connections to pump Disconnect Live Neutral and Earth connections to pump Using an M4 nutdriver or spanner remove the 4 fixing nuts on the underside of the chassis and the pump is free to be lifted from the unit To replace the pump reverse the above procedure IMPORTANT Care should be taken when re fitting Moulded Cover to avoid crimping of any tubing which may affect the Pump Units performance 2 3 2 Control Logic PCB 1 2 COO amp CO Disconnect the Washer Unit from the Pump Unit Using a suitable screwdriver remove the Washer Unit cover by taking out 6 fixing screws on the underneath and 2 fixing screws at the rear of the unit Lift the cover Clear of the chassis Disconnect plug
126. ny electrical equipment should of course be observed with this instrument Do not touch switches or electrical outlets with wet hands Switch instrument off before disconnecting from the mains supply 5 3 5 4 Mechanical and Environmental Mechanical The Wash Head is a free moving mechanical device controlled by the instrument and not directly by the operator It is designed for hands off operation and should be used as such Never reach into the work space while the instrument is performing a wash cycle If it is necessary to stop operation of the equipment press Reset button on the Control Panel Environmental Infectious clinical samples and corrosive fluids are commonly used with this equipment The hands off nature of the system allows the user to wash reaction wells without direct contact with these fluids However wells that have been in contact with potentially hazardous fluid must be handled before and after the wash process and this should be done with utmost care Hand and eye protection should always be worn as well as corrosive resistant laboratory coats When spills contact the Carrier slideway they should be decontaminated with a hard Surface disinfectant Observe normal laboratory procedures for handling potentially hazardous samples Defects and Abnormal Stresses Whenever it is likely that the protection has been impaired the instrument shall be made inoperative and be secured against any unintended operation
127. ockwise to lower the wash head and anticlockwise to raise the wash head Set the tips of the wash head to approx 1 5 mm to 2 0 mm above the height of a microplate on the plate carrier when the wash head is in the raised position Lock the domed nut into position with the locknut When locking the domed nut into position keep it still with the spanner 6 0 and only rotate the locknut anticlockwise using the spanner 5 5 Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 23 Chapter 5 Installation 5 3 3 Mains supply cable Warning Never operate your instrument from a power outlet that has no ground connection Never use a mains supply cable cord other than the Thermo Scientific mains supply cable designed for your region 1 Ensure that the mains switch at the rear of the pump unit is in the OFF position 2 Connect the mains supply cable cord to the mains input socket at the rear of the pump unit If you need to use any other type of mains supply cable than supplied use only cables certified by the local authorities 3 Connectthe instrument to a correctly installed line power outlet that has a protective conductor that is grounded earthed 5 3 4 Changing wash heads You can change the wash head to either 8 way or 12 way Lift the head assembly Fig 4 1 item 8 grasp the wash head Fig 4 1 item 9 at both ends and slide it down in the direction of the tips until it clears the end caps Check that the
128. ontrol peb 006 080BL1 8 Pulley support assembly ans dici 004 058 9 Sliding profile assembly Incl item 10 004 071L 1 10 Mim pitch P O qana kaqa 000767007 11 004 300 Document Release Page 004 006 100 X 3 0 7 ThermoFisher SCIENTIFIC WELLWASH 4 2 SERVICE MANUAL mz Document Release Page 004 006 100 X 3 0 8 ThermoFisher SCIENTIFIC WELLW ASH 4 2 SERVICE MANUAL Spare parts Pump unit assembly POS ITEM CAT 1 Main switch deti 004 087L1 2 Main switch female 004 086L 1 3 O tl t assembly u u 004 511L1 4 1204460 5 Vacuum tube 006 069L 1 6 P mp ssembly 230V aaa 004 067A Pump assembly 115V oue diede tao 004 067 7 control POD P 1514341 Pump control pcb before s n 006 9 5101 006 060 8 125 ml bottle s tube assembly 004 512L1 9 Mans LS 004 085L1 Waste level sensor
129. oth the Wellwash 2 and Wellwash 4 using the catalogue number 934 1 5 2 Electrical Ensure that the mains supply cord supplied with the Unit is always used If a correct type of mains cord is not provided use only cords certified by the local authorities The mains plug should only be inserted in a socket outlet provided with a protective earth contact The protective action must not be negated by use of an extension cord without a protective earth wire Do not replace fuses without first disconnecting the mains supply cord Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuseholders is prohibited When the instrument is connected to the mains supply the opening of covers or removal of components is likely to expose live parts The instrument should be disconnected from all voltage sources by disconnecting the mains supply cord before it is opened for any adjustment replacement maintenance or repair Any adjustment maintenance or repalr of the opened instrument under voltage should be avoided if possible but if unavoidable should be carried out only by a skilled technician aware of the hazard 16 WARNING VOLTAGES DANGEROUS TO HUMAN LIFE ARE PRESENT THIS INSTRUMENT BEFORE REMOVING ANY COVERS DISCONNECT THE INSTRUMENT FROM THE POWER SUPPLY same precautions applicable when using a
130. panner Feeler Gauges Programmable Card Range 0 15 Range 0 8 bar 20 PARTS LIST oENLEY INSTRUMENTS 170 SECTION 4 4 1 Diagrams This section contains 004 851 5 11 32 004 050 004 050 004 051 004 051 004 052 004 052 004 053 004 053 004 054 004 054 004 066 004 066 004 701 004 750 004 751 004 752 004 752 DIAGRAMS the following diagrams Washer Unit General Assembly Items List 1 Sheet Washer Chassis Assembly Items Lists 3 Sheets Washer Cover Assembly Items List 1 Sheet Pump Unit General Assembly Items List 2 Sheets Pump Unit Chassis Assembly Items Lists 2 Sheets Control Logic PCB Assembly Items Lists 4 Sheets Control Logic Circuit Diagram Washer Unit Wiring Diagram Pump Unit Wiring Diagram Plumbing Diagram Items List 1 Sheet OENLEY INSTRUMENTS LTO 21 SECTION 5 TECHNICAL BULLETINS This section contains copies of the Technical Bulletins issued to date which relate to this instrument and whose content has not been covered elsewhere in this manual 5 1 Programmable Switch Card 5 1 1 61 TYPE Sequence 1 Type This switch controls the first type of wash routine to be performed by the Washer Unit Setting Routine 0 No Routine l Aspirate Only 2 Aspirate and Fill move to next Row 3 Aspirate Fill Aspirate move to next Row 4 to F NO Routine 5 1 2 52 TYPE Sequence 2 Type This switch is avaiable if after the SW
131. peats the setting on the Sl TYPE switch As above on this switch setting 0 will in fact give 1 repeat routine setting 1 will give 2 repeat routines 2 will give 3 repeat routines etc Note With S2 TYPE switch set the Aspirate Fill routine the number of repeat routines set is the number of repeat routines per TRAY With S2 TYPE switch set in the Aspirate Fill Aspirate routine the number of repeat routines set is the number of repeat routines per ROW 9 51 PAUS Sequence 1 pause time between Rows This switch controls the length of delay added to the overall time taken to wash a complete Row during the routine set on the Sl TYPE switch The delay can be added incrementally in steps of 0 34 secs from secs up to maximum delay of 5 1 secs according to the O to F setting of the switch for example when the Switch is set at position 8 2 72 secs the plate will take an extra 32 64 secs 12 x 2 72 to process in the 8 way format thereby giving a reliable built in soak time 5 1 6 52 PAUS Sequence 2 pause time between Rows This switch controls the length of delay added to the overall time taken to wash a complete Row during the routine set on the S2 TYPE switch The delay can be added incrementally in steps of 0 34 secs from 0 secs up to a maximum of 5 1 secs according to the 0 to F setting of the switch for example when the switch is set at position 4 1 36 secs the plate will take an extra 16 32 sec
132. pment and waste The proposals for the procedures vary by country Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Fisher Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS Regarding the original packaging and packing materials use the recycling operators known to you For further information contact your local Thermo Fisher Scientific representative Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 48 Chapter 8 Troubleshooting 8 Troubleshooting Warning DO NOT use the instrument if it appears that it does not function properly 8 1 Troubleshooting guide The problems covered below in Table 8 1 are considered as faults that require repair or corrective repair If the installation procedure is carefully followed no faults should arise However if problems occur or reoccur contact authorized technical service immediately Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 49 Table 8 7 Troubleshooting list Chapter 8 Troubleshooting Error Cause Action 1 No POWER ON indicator Mains lead not conne
133. quirements 220 240V AC 50Hz 110 120VAC 60Hz Containers Reagent Bottle Capacity Waste Bottle Capacity Fluid Consumption Wash Fluid Consumption Priming Consumption Pressure Dimensions Washer Unit Pump Unit Weight Washer Unit WW004 W Pump Unit WW004 P Temperature Operating Range Full Specification Range Storage Temperature Range Relative Humidity Safety Wellwash 4 fullfills the following requirements 220 240 VAC 50 Hz 110 120 VAC 60 Hz 240 VA Continuous 1 off 0 5A anti surge fuse in rear of Washer Unit 1 off 1 0A anti surge fuse in rear of Pump Unit 1 off 0 5A anti surge fuse in rear of Washer Unit 1 off 2 0A anti surge fuse in rear of Pump Unit 2 ltr 2 ltr approx 150ml complete microplate approx 15ml Max pressure on Vessel 7psi self limiting Alem x 22cm x 12cm 30cm x 18cm x 16cm 4 5 Kg 5 5 Kg 5 to 40 C 15 C to 2576 10 C to 70 C 90 max 348 Cat 1 amp DIN 57411 Pt 1 VDE 0411 Pt 1 Safety Class 1 IEC 1010 1 including Amendments 1 and 2 Un The safety specifications are met also under the following environmental conditions in addition or in excess to those stated in the operating conditions Altitude up to 2000 m Mains supply fluctuations 10 Installation category according to 664 1 note 1 overvoltage category Pollution degree 2 according to IEC 664 1 note 2 Notes 1 Installation category overvoltage ca
134. ram Card for the procedure required Plug it into the slot on the right hand side of the Washer Unit Lift the Head and slide the Plate Carrier onto the stainless steel slideway so that the Reservoir is under the Wash Head Press the PRIME button The Wash Head will operate three times up and down dispensing into and aspirating from the Priming Reservoir finally stopping in the DOWN position Place a microplate onto the Plate Carrier When using a 12 way Wash Head well Al the microplate should be positioned in the top left hand corner Select on the Control Panel the row number of the last row to be washed eg when the button 4 is pressed rows 1 to 4 will be washed NOTE When the 12 way Program Card is installed buttons 9 12 are inoperative Press the START button which will set the washing cycle into operation The carriage will be drawn into the Washer filling and aspirating each row to the sequence selected on the Program Card At the end of a sequence rows will be left substansially dry and the Carriage will stop with the Priming Reservoir under the Wash Head 3 3 1 OPERATING PROCEDURES Controls and Indicators The following paragraphs describe the Controls and Indicators on the Wellwash 4 3 1 1 POWER ON OFF Switch This two position switch at the rear of the Pump Unit controls the electrical power to both the Pump and Washer Units POWER Indicator This green LED is located at the bottom left of the Contro
135. rect wash head 8 way or 12 way is fitted Check that the plate is placed properly onto the plate carrier Fig 5 16 Select the row number of the last row to be washed If no row is selected the whole plate will be washed The instrument performs the wash sequence After pressing the START button there may be a delay of up to 15 seconds before the run begins to allow the reagent and waste bottles to reach the working pressure The plate carrier will advance into the washer aspirating and filling selected rows in the sequence determined by the program card fitted On completion of the protocol the plate carrier will come to rest with the wash head lowered into the priming reservoir Rev 2 3 Cat no 1507340 27 Warning 0 Caution Warning Warning 9 Warning Note Note e Caution Caution Caution Note Chapter 6 Operation If the instrument is used in a manner not specified the manufacturer the protection provided by the instrument may be impaired See Section 9 1 General specifications Handle the microplate with care to avoid any contamination of the instrument and the subsequent specimens The contamination of the specimen will lead to incorrect results If the contents of the microplate does spill however follow the decontamination procedure presented in Section 7 3 Also follow the system supplier s instructions in regard to the reagent kit and specimen handling in the event of contaminat
136. riate washing reagent and tighten the bottle cap firmly Fig 5 12 Select either one of the preprogrammed wash cards or the programmable switch card Section 6 5 Before inserting any program card ensure that it is clean and dry Wiping the surfaces with a soft dry cloth will suffice If any liquid is present on the card a malfunction of the program may occur Position the plate carrier on its slideway with the priming reservoir to the rear under the wash head see Fig 5 13 The POWER indicator on the keypad Fig 4 2 illuminates and a countdown sequence will begin on the row indicator LEDs The PRIME indicator flashes until you prime the system The priming function is complete when the PRIME indicator turns OFF If no further key entry is made within fifteen seconds of priming then the pump will automatically switch OFF The instrument will then enter the idle state waiting for instructions from the keypad and program card Check that the correct wash head 8 way or 12 way is fitted Check that the plate is placed properly onto the plate carrier Fig 5 16 Select the row number of the last row to be washed If no row is selected the whole plate will be washed The instrument performs the wash sequence Rev 2 3 Cat no 1507340 64 Appendix Certificate of Decontamination Appendix Certificate of Decontamination The decontamination procedure is required prior to shipping the instrument to Thermo Fi
137. rior to carrying out any wash function 6 8 Emergency situations In case there is any abnormal situation during operation such as fluids spilling inside the instrument follow these steps 1 Switch OFF the instrument 2 Unplug the instrument from the mains 3 Perform appropriate corrective actions However do not touch the interior of the instrument 4 Contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 35 Chapter 7 Maintenance 7 1 Regular and preventive maintenance 7 1 1 Service contracts It is strongly recommended that this instrument be regularly maintained and serviced every twelve 12 months on a contract basis by trained service engineers of the manufacturer This will ensure that the product is properly maintained and that it gives trouble free service For more details contact Thermo Fisher Scientific s service department 7 1 2 Maintenance check list The check list below contains a recommended maintenance schedule Table 7 1 Maintenance check list Item gt 5 5 o c gt Wipe the case of the instrument e Cover the instrument with the dust cover after use e Clean the instrument and the keyboard with a cloth dampened with mild detergent followed deionized distilled aqua See Sections 7 1 3 an
138. rks 1 004 101b CHASSIS PUMP UNIT 1 2 3 4 004 067b PUMP SUB ASSEMBLY 1 5 004 330 MODIFIED ELBOW CONNECTOR 1 8 000709004 SOCKET FUSE HOLDER 1 7 000716005 FUSE 2A A S 2 8 9 000709008 MAINS SOCKET 1 10 11 50 019 SILICONE TUBE 630 mm 12 004 062 VACUUM TUBE ASSEMBLY 1 13 000757017 ELBOW CONNECTOR 1 14 000706037 SWITCH 1 15 000727005 PIGGY BACK CRIMP CONNECTOR 2 16 000727002 SHROUDED RECEPTICAL 3 16 6 17 18 000743017 FRICTION BUSH 2 19 000780007 4 2 S PROOF 20 000891001 EARTH SYMBOL 1 B 21 22 000827003 SLEEVING H20 x 20 4 23 C6124 WIRE 20 AWG 10 30 GREY 310 mm 24 6144 WIRE 18 AWG 16 30 GREY YLLW 315 mm 25 C6121 WIRE 20 AWG 10 30 BLUE 310 mm 26 122 WIRE 20 AWG 10 30 BROWN 310 mm 27 C6126 WIRE 20 AWG 10 30 RED 310 mm 28 29 000543901 CABLE TIE BASE 6 30 000543428 CABLE TIE 12 31 000892012 LABEL TRANSIT SCREW 1 32 33 000757031 BARBED CONNECTOR 1 34 35 000789008 FEET 4 36 37 000802449 SCREW M3 x 8 850 CSK HD 4 38 000801831 SCREW x 3 8 HD 5 POZ 3 39 40 41 42 43 44 45 000804076 WASHER M3 WAVE 7 46 47 48 000804078 WASHER M4 WAVE 4 WHOLE OR PART MUST NOT BE MADE WITHOUT THE WRITTEN CONSENT 4336 22 L 1995 IIR EE NI EIN MN for Org No 004 054B CHASSIS ASSEMBLY PUMP UNIT 110 120V 60HZ DENLEY Billingshurst Sussex RH14 9EY England ty Remarks 51 000803008
139. roduct is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC p E E B Warning markings used in the documentation Warning Risk of electric shock AN Warning Biohazard risk Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of N performance or function in a specific application Other markings used in the documentation Note Marks a tip important information that is useful in the optimum 1 operation of the system or an item of interest Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 Chapter 2 About the User Manual 2 About the User Manual This User Manual has been written for the actual user laboratory technician and provides information on the Thermo Scientific Wellwash 4 Mk 2 microplate strip washer including installation and operating instructions Read the manual in its entirety prior to operating the instrument This User Manual has been designed to give you the information you need to e Review safety precautions e Install the Wellwash 4 Mk 2 e Carry out washing procedures e Perform basic maintenance procedures e Troubleshoot the instrument performance e Maintain the instrument This User Manual also describes features and specifications of the Wellwash 4 Mk 2 hardware and on board software Chapter 6 explains the operating procedures
140. rol switches or a preprogrammed wash card 1 Liquid dispensing into wells microplate or microstrip 2 Liquid aspiration from wells microplate or microstrip With their unique and proven design the coaxial wash heads of the Wellwash 4 Mk 2 ensure excellent washing efficiency and low residual volumes of less than 5 ul For clean delivery of washing buffer the inner dispensing tubes are made of stainless steel A continuous vacuum in the outer tube prevents cross contamination and ensures complete liquid removal For fast and accurate selection of routine protocols the Wellwash 4 Mk 2 comes with four standard program cards 1 to 4 washing cycles as well as a programmable card This switch card permits a quick selection of all wash parameters including soak times and final wash protocols The quick release bottle caps of the Wellwash 4 Mk 2 ensure easy buffer changes An additional rinse bottle can be connected making it easier to purge wash buffer from the wash head or change to a different wash buffer Automatic pump shutdown reduces wear and noise levels If unused for 30 seconds the wash pump of the Wellwash 4 Mk 2 automatically switches off 3 3 Advantages of using Wellwash 4 Mk 2 The Wellwash 4 Mk 2 provides several advantages relating mainly to the principle of operation in that it has e Unique coaxial wash heads for trouble free washing and low residual volumes 8 12 way interchangeable wash heads e Easy to progr
141. rough the pinch valve a few millimeters the valve will now pinch into a new position See Fig 7 6 pressure by slightly opening the wash bottle cap Let the hissing sound Warning Never press the black top of the pinch valve without first releasing the end prior to pushing the valve top WELWASM awn Fig 7 6 Unpinching the tube If the washer is run now the liquid will flow normally It may be necessary to replace the tube after extended periods of use due to fatigue Use the spare tubes supplied with the instrument Fig 5 2 item 3 1 Switch OFF the instrument and make sure there is no pressure in the wash bottle by opening the wash bottle cap slightly 2 Press the black top of the valve to release the tube and slide the tube gently out of the pinch valve through the slit on the side Disconnect the tube from both ends and replace with a new one 4 Press the black top of the valve and slide the new tube back into the valve through the slit Make sure the tube is properly in place through the valve and is not pinched especially on the rear side of the valve Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 42 Chapter 7 Maintenance 7 1 9 Unclogging tips The dispense aspirate tips of the wash head may become clogged with salt deposits To clear them switch OFF the washer lift up the wash arm assembly and unclog the tips using the small de clogging tool provided see Fig 7 7
142. rovide positive location and indexing facilities for a 96 well microplate 2 To control all aspects of the dispensing and aspirating of wash fluid via interchangeable programme cards Housed within the unit are the following controls and components Main Power Inlet Control Keyboard Mains Filter Transformer Control Logic PCB Carriage Indexing Motor and Belt Drive Head Lift Motor Pinch Valve Dispensing Head Vacuum Switch Manifold Block and associated Tubing The Washer Unit is connected to the Pump Unit by fitting the Vacuum Fail tubing to the Vacuum Fail Switch connecting the electrical signal plugs then pushing the units together using the Mains connector and the Location Dowels for alignment Located inside the case is the drive motor for indexing of the microplate which is linked via a drive belt and pulleys to a magnetic linkage system on the microplate carrier Raising and lowering of the head is achieved by a second motor driving an eccentric block linked by a sprung loaded rod Dispensing and aspirating is achieved by inserting a suitable programme card selecting the number of rows to be washed and pressing the start button 004 851 5 11 92 B3ENLEY INSTRUMENTS LTD SECTION 1 THEORY OF OPERAT 1 4 Control Logic PCB Diagrams Required 004 701 Circuit Diagram 004 750 Wiring Diagram 1 4 1 Control Logic PCB Description The PCB is located inside the Washer Unit the function of which when fitted with th
143. rrect type of mains cord is not provided use only cords certified by the local authorities Connect the mains plug to the mains power socket 8 2 3 6 2 4 Changing Wash Heads Lift the Head Assembly Figure 1 10 grasp the Wash Head Figure 1 7 at both ends and slide it down in the direction of the tips until it clears the end caps Check that the two O rings in each end cap are in position and undamaged To install the alternative Wash Head slide it upwards on the slots making sure that it is fully engaged NOTE The Wash Heads will only fit into the end slots when the raised flange on the Wash Head is to the front Operational Check following procedure should be completed to confirm the correct functioning of the Washer prior to normal use 2 4 1 2 4 2 2 4 3 2 4 4 2 4 5 2 4 6 2 4 7 2 4 8 Check that the Wash Head is correctly located by means of its guide slots in the Head Assembly and that both Bottle Caps are firmly tightened Switch ON the mains power supply and depress the POWER ON switch positioned on the rear of the Pump Unit The POWER ON indicator on the Control Panel will be illuminate and a countdown sequence will begin on the Row indicators Row 12 indicator will illuminate first followed by Row 11 Row 10 Row 9 and so on at one second intervals until the prime LED is reached The prime LED indicator continues flashing to prompt the user to prime the system Select an appropriate Prog
144. s EIA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to measure or detect a substance using antibody antigen reactions EN European Norm EU European Union IEC International Electrotechnical Commission Microplate A rigid or framed polystyrene plate with microwells in different well formats e g 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as EIA or ELISA Priming Completely filling the pump tubing and syringe with bubble free fluid to allow sustained reproducible pumping action The air in an unprimed line acts as a spring adversely affecting accuracy and precision Reagent A substance or solution used to produce a characteristic chemical reaction sample that allows an analyte to be detected and measured RS 232C EIA approved standard used in serial data transmission covering voltage and control signals The data is transferred serially one digital bit at a time via one path but some control signals can be transferred simultaneously via parallel paths 13 1 Keywords for web pages 13 2 Literature EIA plate ELISA sandwich assay enzyme immunoassay separation technique s liquid handling sandwich assay microplate solid phase assays microplate washing Thermo Scientific Microtiter plate Thermo Fisher Scientific microwell plate washing Newman
145. s 12 x 1 36 to process in the 8 way format thereby giving a reliable built in soak time 3 1 7 PREWASH POSTWASH Both the Prewash and the Postwash switches control the timing and hence the Svolume S liquid dispensed during the routines set on 51 TYPE and S2 TYPE switches The switches work on an overlapoing time base and so with certain combinations of Prewash and Postwash switch settings no dispense will occur To avoid this situation it is recommended that the Prewash switch is set to position 7 as this has been found to be the optimum position for most users and that the Postwash switch be used in the range of positions from 5 to F to avoid the no dispense situation 004 831 5 11 92 DENLEY INSTRUMENTS LTO 23 SECTION 5 TECHNICAL BULLETINS 5 1 8 PREWASH Delay time from Head down position to liquid dispense This switch controls the length of delay between when the Dispense Head is at the bottom of the plate well and the liquid starting to dispense during the routines set on Sl TYPE and 52 TYPE switches The delay can be added incrementally in steps of 0 04 secs from a minimum setting of O ll secs to a maximum of 0 71 secs Setting the switch to position 0 gives 0 11 secs delay position 1 gives 0 15 secs delay position 2 gives 0 19 secs delay etc Note Although the Prewash timing is adjustable customers are advised to leave the switch set to Position 7 as during our own and independent testing
146. s can be potentially infectious Dispose of all used microplates microstrips disposable gloves syringes disposable tips etc as biohazardous waste 7 3 Decontamination procedure gt gt gt Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed A decontamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended that the complete decontamination procedure be performed before relocating the instrument from one laboratory to another See Section 7 4 How to pack for service Decontamination is not required for the proper functioning of the instrument Example of decontaminants e Ethanol 7096 e Virkon solution 1 396 e Glutaraldehyde solution 4 Chloramine e Microcide SQ 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect the enzyme being used in EIA tests in a negative way resulting in bad test results Caution Do not use sodium hypochlorite as a decontaminant The wash head will be damaged by it Warning A
147. s from and PLS Disconnect Mains Supply connections to PCB at TB3 Disconnect 15 Volt Power Supply to PCB at TB4 Disconnect Motor Leads at TBl TB2 and TB3 Remove Nylon M3 fixing screw from REG 2 Lift the Idler Pulley and Drive Belt clear from the mounting shaft Lift Sliding Profile Assembly clear of the Guiding Profile Remove 6 off M3 fixing nuts and washers The PCB is now free to be lifted from the chassis To replace the PCB reverse the above procedure IMPORTANT Care should be taken when re fitting Moulded Cover to avoid damaging Indicator LED s 004 851 5 11 92 9 DENLEY INSTRUMENTS LTD 12 SECTION 4 MAINTENANCE 2 3 3 Indexing Mechanism Assembly 1 2 gt C Ul M Disconnect the Washer Unit from the Pump Unit Using a suitable screwdriver remove the Washer Unit cover by taking out 6 fixing screws on the underneath and 2 fixing screws at the rear of the unit Lift the cover clear of the chassis Lift the Idler Pulley and Drive Belt clear from the mounting shaft Lift Sliding Profile Assembly clear of the Guiding Profile Release Drive Belt from Drive Pulley Grasp Sliding Profile Assembly and lift clear of the Guiding Profile The Guiding Profile can be removed by unscrewing the 3 x M3 fixing screws in the underside of the chassis To replace the Indexing Mechanism reverse the above procedure IMPORTANT Care should be taken when re fitting Moulded Cover to avoid
148. sher Scientific Oy e g for repair If for any reason the instrument is shipped back to Thermo Fisher Scientific Oy it must be accompanied by a dated and signed Certificate of Decontamination which must be attached to the outside of the package containing the instrument See Section 7 3 Decontamination procedure Failure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure that they fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals Please include decontaminating solution
149. te In addition to standard program cards for routine wash procedures cards can be provided for special routines incorporating any of the above functions See Section 7 Program Selection Choose appropriate Card for the wash routine Plug the Card into the slot located at the base of the right hand of the Wash Unit NOTE Before inserting any Program Card ensure that it is clean and dry wiping the surfaces with a soft dry cloth will suffice If any liquid 15 present on the Card a malfunction of the Program may occur Row Selection Select on the Control Panel the row number of the last row to be washed eg when button 4 is pressed then rows 1 to 4 will be washed NOTE When the 12 way Program Card is installed buttons 9 to 12 are inoperative 3 2 7 3 2 8 3 2 9 3 3 Washing Microplate Select the number of rows to be washed remembering that it is only necessary to press the button assigned to the last row to be washed Pressing any other button will override the previous selection and automatically reset the program Press the START button which will initiate the wash sequence The Plate Carrier will advance into the Washer aspirating and filling selected rows in the sequence determined by the Program Card fitted On completion of the protocol the Plate Carrier will come to rest with the wash head lowered into the Priming Reservoir NOTE Pressing the START button without entering a program will automatically cause t
150. tegory defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 2 Pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Both of these affect the dimensioning of the electrical insulation within the instrument Conformance to the EU Directives Wellwash 4 bearing the CE 89 336 EEC as amended by 92 3 relating to mark conforms to the Electromagnetic Compatibility following EU Directives 73 23 EEC Low Voltage Directive Performance specifications EN 55014 used to verify conformance to IEC 801 3 3V m the Directives above IEC 801 2 4 kV CD 8 kV AD IEC 801 4 1 kV 1010 1 EN 61010 1 Including Amendments 1 and 2 Catalogue Description Wellwash 4 Semi Automatic microplate Washer comprising Pump and Wash Units The instrument is supplied with both 8 way and 12 way Wash Heads 4 Program Cards and an
151. the Drive Belt should be such that it is not tight enough to cause undue strain or stalling of the Drive Motor but not so loose as to cause jumping of teeth on the pulleys Adjustment is performed by loosening the M4 fixing screw on the underside of the chassis and moving the position of the Pulley Mounting Block to obtain the correct tension and re tightening the fixing screw Re assemble by reversing the above steps IMPORTANT Care should be taken when re fitting Moulded Cover to avoid damaging Indicator LED s 004 851 5 11 92 9 DENLEY INSTRUMENTS LTD SECTION 2 2 2 Fault Finding MAINTENANCE Given below is a short form fault finding chart for the Wellwash 4 which defines some hypothetical problems PROBLEM POSSIBLE CAUSE No POWER indicator VAC FAIL light continuously illuminated Mains Lead not connected Fuse of Washer or Pump Unit blown Pump Unit POWER switch is OFF Pump amp Washer not properly connected Air Leaks Constricted tubing Pump Failure Incorrectly adjusted Vacuum Switch ACTION Connect lead to an appropriate supply Replace fuse Switch POWER switch ON Push Pump amp Washer Units firmly together Check bottle caps for leaks and tighten as required Check tubing for kinks collapse Check that Pump Unit is switched ON Check that Pump Unit is connected to the Washer Unit correctly Re calibrate Vacuum
152. the wash bottle Black Pressuring wash reagent from the wash bottle to the washer Yellow 6 unit Red Vacuum feed from the pump to the waste bottle Blue Waste feed from the washer unit to the waste bottle Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 19 Chapter 5 Installation 2 Connectthe pressure feed from the pump on the right to the wash bottle using the black coded tube 3 Connect the vacuum feed tube red from the pump to the waste bottle Fig 5 9 item 2 4 Connectthe liquid level sensor cord from the liquid trap bottle to the waste bottle Make sure the black connector is firmly in place Fig 5 9 item 1 inserted the black connector has to be pushed down all the way The waste bottle may otherwise overfill and cause the liquid trap bottle to start filling If at that point the liquid trap bottle is not emptied the overflowing waste liquid can enter the pump unit and damage it Caution The liquid level sensor does not function if the connector is not properly BOTTLE FU 2 BOTTLE MUST EMPTIED WHEN Fl REACHES THIS LEVE 1 Liquid level sensor connector 2 Vacuum feed tube 3 Liquid trap bottle cover Fig 5 9 Liquid level sensor system 5 Check that the tubes are properly connected to the pinch valve under the wash arm assembly on the washer unit Fig 4 1 item 14 The thin tube going through the valve should not be pinched by the edges of the valve Th
153. tions warnings 52 Liquid reservoir 52 Electrical et eto ep teet a erp 52 Mechanical and 1 1 4 0 0004 4 14 53 Defects and abnormal stresses 1 4 44 2000 74444404444441004 44119 54 Before Operation cs i l w ree TRE ae FORE ver Ea Eire 54 InStallatiOEl RE 54 e Ea aite TR 54 er 54 Technical 5 55 General 1 55 Safety 56 In conformity with the 2 0 110 1 nnne nnns 56 Ordering 57 LIS GPS6866SsSDrleS 57 List of recommended spare parts 111 1 57 pea Eve 58
154. to cover the last row to be washed 6 that if removable well microplates are being used there are no missing wells in the rows to be washed and that all wells are level Wash Head Height To accomodate the differences in size and volume capacity of manufacturers microplates the height of the Wash Head is adjustable This means that a positive meniscus can always be obtained without the unnecessary waste of Wash Reagent The Wash Head is factory set at a height to suit the most commonly used microplates should adjustment be required follow these instructions 1 Adjustment is performed using the spanners supplied with the instrument 2 Located on the left hand side of the Washer Unit immediately above the Control Panel is a stainless steel Head Lift Rod At the top of the Rod is a domed nut with a lock nut underneath 13 3 By loosening the nut lock height adjustment be performed by rotating the domed nut clockwise to lower the Wash Head and anti clockwise to raise the Wash Head 4 Setthe Tips of the Wash Head to approx 1 5 mm to 2 0 mm above the height of a microplate on the Plate Carrier when the Wash Head is in the raised position 4 4 Disinfection WARNING IF LOCAL OR LABORATORY REGULATIONS PRESCRIBE REGULAR DISINFECTION IT IS ADVISABLE NOT TO USE FORMALDEHYDE SINCE EVEN SMALL TRACES OF FORMALDEHYDE AFFECT THE ENZYME BEING USED IN MICROELISA TESTS IN A NEGATIVE WAY RESULTING IN BAD TEST RESULTS
155. uld be read with reference to the relevant drawings and diagrams These descriptions are intended to supply sufficient information for an understanding of the operation of the washer to be gained to aid diagnostic trouble shooting 1 1 General Description The WWOO4 Wellwash 4 is designed to wash coated wells a 12 x 8 format microplate It comprises a Pump Unit and Washer unit which are connected by power signal liquid and air lines The Pump Unit consists of a double headed vacuum pump with its associated tubing housed within a polyurethane moulding Mounted on either side of the moulding are high density polythene Wash Reagent and Waste Bottles At the rear of the Pump Unit is the main power on off switch Located on the left hand side of the Washer Unit is the Control Panel under which the microprocessor and associated control electronics are housed Above the Plate Carrier slideway is the Wash Head which be either 8 or 12 Way Plate Carrier locates the plate accurately beneath the Wash Head and incorporates a Priming Reservoir at its leading edge The Carrier is indexed forward by means of a magnetic coupling to the drive system housed inside the Washer 004 851 5 11 92 9 DENLEY INSTRUMENTS LTD SECTION 1 ___ OF OPERATION 1 2 Pump Unit Assembly Diagrams Required 004 053 General Assembly 004 053 Items List 1 Sheet 004 054 Chassis Assembly 004 054 Items Lists 6 Sheets 004 751 Wiring Diagr
156. used Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 65 Appendix Wellwash 4 Mk 2 Feedback Form Please send to Thermo Fisher Scientific Oy Fax 358 9 32910415 Appendix Wellwash 4 2 Feedback Form Cat Serial no PURCHASED BY PURCHASED FROM Company Organization Distributor Department Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Mr Mrs Ms Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions Instrument User manual LH Operational reliability Design LH Operational costs Customer support Further instrument system developments desired Further applications desired Where did you first learn about the product Would you like to receive information about other Thermo Fisher Scientific products Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 66 55 5 Appendix D Addresses For the latest information on products and services visit our worldwide web sites on the Internet at http www thermo com Manufactured Distributed by Thermo Fisher Scientific Oy Box 100 FI 01621 Vantaa Finland
157. wash head holders 3 Waste bottle 10 Priming reservoir 4 Wash bottle 11 5 Bottle caps 12 Plate carrier 6 Liquid level sensor 13 Program 7 Liquid trap bottle 14 Pinch valve Fig 4 1 Wellwash 4 Mk 2 microplate washer The pump creates pressure to the wash bottle Fig 4 1 item 4 allowing the wash liquid to be dispensed through the pinch valve Fig 4 1 item 14 into the microplate wells and a vacuum to the waste bottle Fig 4 1 item 3 to aspirate the used wash liquid into the waste bottle Thermo Scientific Wellwash 4 Mk 2 User Manual Rev 2 3 Cat no 1507340 11 Chapter 4 Functional Description 4 1 Controls and indicators PRIME 2 3 4 e 5 6 8 e 10 12 e 4 2 is a description of the controls and indicators on the Wellwash 4 Mk 2 POWER ON OFF switch This two position switch at the rear of the pump unit controls the electrical power to both the pump and washer units PRIME push button A momentary action switch that starts the PRIME function An audible tone will sound when pressed to confirm operation PRIME indicator This yellow LED light emitting diode located to the right of the PRIME button flashes to indicate that the PRIME function is required and when continuously ON indicates that the washer is primed and ready for use The indicator will turn off after a while
158. wire of the mains cord This is connected to the instrument before the line and neutral supply connections when the supply plug is inserted into the socket on the back of the instrument If the final connection between this and the supply is made elsewhere the user must ensure the ground connection is made before line and neutral 1 2 3 Live Parts The instrument is safe to operate with covers fitted and these must not be removed under normal usage The covers protect the user from live parts and they should be removed only after switching off the instrument and disconnecting the mains supply cord and by suitably qualified personnel for maintenance and repair purposes WARNING VOLTAGES DANGEROUS TO HUMAN LIFE ARE PRESENT IN THIS INSTRUMENT BEFORE REMOVING ANY COVERS DISCONNECT THE INSTRUMENT FROM THE POWER SUPPLY 1 2 4 Operating Temperature The instrument is designed for safe operation in an environment having an ambient temperature of between 5 C and 40 C RH 90 and an altitude below 2000 m It will operate with full accuracy within a temperature range of 15 C to 25 C NOTE The use of the instrument in strong direct sunlight or next to radiators and other heat sources may markedly increase the temperature at the instrument and this should be taken into account when deciding where the instrument should be located and operated 13 Specification Input Voltages Power Consumption Operating Cycle Fuse Re

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