Home

Agilent 1290 Infinity Autosampler User Manual

1. Solvent cabinet b Pump j i p e e r ee j q NU Autosampler Thermostat for the ALS optional Figure 9 Recommended two stack configuration for 1290 Infinity front view Agilent 1290 Infinity Autosampler User Manual 37 3 Installing the Autosampler Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Instant Pilot Analog detector signal tional optional EN i PA CAN Bus cable Thermo cable optional AC Power Figure 10 Recommended two stack configuration for 1290 Infinity rear view 38 Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler 3 Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the
2. Notices Agilent Technologies Inc 2011 2014 2015 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64226 90001 Rev C Edition 02 2015 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnis
3. 26 Agilent 1290 Infinity Autosampler User Manual Site Requirements and Specifications 2 Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 15 5 kg 34 2 lbs Dimensions height x width 200 x 345 x 440 mm 8 x 13 5 x 17 inches x depth Line voltage 100 240 V 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 200 VA 200 W 683 BTU Maximum Ambient operating 4 55 C 39 131 F temperature Ambient non operating 40 70 C 40 158 F temperature Humidity lt 95 r h at 40 C 104 F Non condensing Operating altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15092 ft For storing the module Safety standards IEC CSA Installation category Il Pollution degree2 For indoor use only UL Agilent 1290 Infinity Autosampler User Manual 27 2 Site Requirements and Specifications Performance Specifications Table 2 Performance specifications G4226A Type Specification Comment Injection range Precision Pressure range Sample viscosity range Sample capacity 0 1 20 pL in 0 1 pL increments 0 1 40 pL in 0 1 pL increments if 40 pL loop is installed 0 1 120 pL in 0 1 pL increments with 1290 Infinity large volume injection kit hardware modification required pressure range up to 1200 bar 0 1 100 pL in 0 1 pL increments with 100 uL upgrade kit G4214A
4. NOTE The module was shipped with default configuration settings For changing these settings refer to section Setting the 8 bit configuration switch Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler 3 Flow Connections to the Autosampler Parts required Description 1 System Capillaries and tubing from Accessory Kit 1 ChemStation and or Instant Pilot G4208A with the appropriate revisions see Performance Specifications on page 28 Preparations Autosampler is installed in system This procedure shows the Autosampler outside of a system In an Agilent 1290 Infinity LC System the Autosampler is located between a G4220A Binary pump below and the G1316C TCC SL above see Optimizing the Stack Configuration on page 34 Install the capillary from the pump outlet into the port 1 of the injection valve 1 Open the front cover by pressing the button on the right 2 side of the module Agilent 1290 Infinity Autosampler User Manual 45 3 Installing the Autosampler Flow Connections to the Autosampler 3 Install the capillary from the port 6 of the injection valve to the TCC NOTE The Autosampler can only be operated with the front and side covers closed 46 Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 4 Using the Module Leak and Waste Handling 48 Preparing the Autosampler 49 Set
5. 8 Test Functions Sample Transport Self Alignment The sample transport self alignment uses predefined positions on the well plate tray to calibrate the positioning of the needle The sample transport self alignment is required to compensate for larger deviations in positioning the needle carrier The sample transport self alignment is required after disassembling the system or when you exchange the sample transport the sampling unit the tray or the MTP main board This function is in the calibration screen of the Lab Advisor When After disassembling the module or by larger deviations in the positioning of the needle Preparations Well plate tray needs to be installed and empty 124 Agilent 1290 Infinity Autosampler User Manual Test Functions 8 Sample Transport Self Alignment 1 Run the Transport Alignment with the Agilent Lab Advisor for further information see Online Help of user interface Agilent Lab Advisor Diagnostic Results Stop Test G Print Results Test Name Transport Alignment ipti The sample transport alignment with the sampling unit and the well plate tray is required to compensate larger deviations in Module G4226A LP00055010 positioning the needle carrier It is required after disassembling the system or after exchanging either the Calibration Descriptions 5 Approx Time Not defined sample transport the sampling unit the MTP main board or G13674 B C G13774 G2258A Status Running the well plate s
6. hardware modification required up to 600 bar Typically lt 0 25 RSD from 5 20 pL Typically lt 0 5 RSD from 2 5 pL volume Typically lt 0 7 RSD from 1 2 pL volume Up to 1200 bar Up to 600 bar 0 2 5 cp Capacity 2 x well plates MTP 10 x 2 mL vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate 100 Micro vial tray plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 mL in 2 x 27 Eppendorf tube plate Measured with injections of benzylalcohol with 1290 Infinity large volume injection kit installed with 100 uL upgrade kit G4214A installed Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity 28 Agilent 1290 Infinity Autosampler User Manual Site Requirements and Specifications 2 Table 2 Performance specifications G4226A Type Specification Comment Injection cycle time Typically lt 21 s using following standard conditions Default draw speed 100 pL min Default eject speed 100 pL min Injection volume 5 pL Carry Over Typically lt 0 004 Using the following conditions Column Agilent ZORBAX SB C18 2 1 x 50 mm 1 8 um 827700 902 Mobile Phase A 0 1 TFA in water B 0 1 TFA in Acetonitrile Isocratic B 35 Flow rate 0 5 mL min Temperature 25 C Wavelength 257 nm Sample 1200 ng pL Chlorhexidin
7. 5181 1516 5181 1519 LAN cables p n 5023 0203 5023 0202 Identifying Cables 11 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n G1103 61611 RS 232 cables p n G1530 60600 RS232 61601 5181 1561 Description General Purpose Cable Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1290 Infinity Autosampler User Manual 175 11 Identifying Cables Analog Cables 176 One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 7 oe 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog an Center Center Analog Agilent 1290 Infinity Autosam
8. Change Needle 127 Change Loop Capillary 127 Arm Position 128 Change Needle Carrier 128 Change Metering Device 129 Injector Steps 130 Step Commands 131 This chapter describes the tests for the module ot Agilent Technologies 117 8 Test Functions Introduction All tests are described based on the Agilent Lab Advisor Software B 01 03 or above Other user interfaces may not provide any test or just a few Table 6 Interfaces and available test functions Interface Comment Available Function Agilent Instrument Utilities Maintenance tests available System Pressure test Sample transport Self Alignment Agilent Lab Advisor All tests are available System Pressure test Sampler leak test Sample transport Self Alignment Agilent ChemStation No tests available Pressure Adding of pressure to e Pressure ripple chromatographic signals e Temperature mainboard possible Agilent Instant Pilot Some tests are available System Pressure test Sample transport Self Alignment For details on the use of the interface refer to the interface documentation 118 Agilent 1290 Infinity Autosampler User Manual Test Functions 8 System Pressure Test The test determines the leak rate of the system between pump outlet valves and a blank nut The blank nut can be positioned at different locations in the system before the flow cell to determine and verify the leak rate of individual modules and components The test allows fo
9. Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Error ID 0070 Error Information 7 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Agilent 1290 Infinity Autosampler User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 99 7 Error Information Lost CAN Partner Error ID
10. Response Time Data Rate Peak width at half Response Signaldata Scandatarate Scandatarate Scandatarate Scan data rate height min s rate Hz HZ HZ HZ HZ 126 pts sca lt 251pts sca 501 pts sca gt 501 pts sca n n n n lt 0 0016 0 016 1602 1602 80 40 20 gt 0 0016 0 03 1602 1602 80 40 20 gt 0 003 0 062 80 80 80 80 40 gt 0 006 0 12 40 40 40 40 40 gt 0 012 0 25 20 20 20 20 20 gt 0 025 0 5 10 10 10 10 10 gt 0 05 1 0 5 5 5 5 5 gt 0 10 2 0 2 5 2 5 2 5 2 5 2 5 gt 0 20 4 0 1 25 1 25 1 25 1 25 1 25 gt 0 40 8 0 0 625 0 62 0 625 0 625 0 625 gt 0 85 16 0 0 3125 0 31 0 3125 0 3125 0 3125 1 2 64212A only 86 Values in the User Interface may be rounded Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 How to Achieve Lowest Carry Over Carryover is measured when residual peaks from a previous active containing injection appear in a subsequent blank solvent injection There will be carry over between active injections which may lead to erroneous results The level of carryover is reported as the area of the peak in the blank solution expressed as a percentage of the area in the previous active injection The Agilent 1290 Infinity autosampler is optimized for lowest carryover by careful design of the flow path and use of materials in which sample adsorption is minimized A carryover figure of 0 002 should be achievable even when a triple quadrupole mass spectr
11. 1 0 0 0 0 0 1 204 Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument per
12. 148 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Removing the Metering Seal When When poor injection volume reproducibility or when metering device analytical head is leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2392 4 mm Hex key G4226 43800 Seal insert tool Parts required p n Description 1 0905 1717 Metering seal 1 Inthe LabAdvisor User Interface start Service amp 2 Open the front door Diagnostic and select Maintenance Positions function In this section the Change Metering Device function can be found Agilent 1290 Infinity Autosampler User Manual 149 9 Maintenance Removing the Metering Seal 3 Remove the two attached capillaries with a inch 4 Unscrew alternately the two fixing screws with a 4 mm wrench hex key 5 Pull the metering device analytical head away from the 6 Remove the two fixing screws at the base of the metering sampling unit device analytical head 150 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Removing the Metering Seal 7 Remove the head body 8 Carefully remove the metering seal using the steel side of the insert tool Clean the chamber and ensure all particulate matter is removed Insert tool Agilent 1290 Infinity Autosampler User Manual 151 9 Maintenance Installing the Metering Seal When After removing the metering seal Tool
13. Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Agilent 1290 Infinity Autosampler User Manual 25 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 27 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation
14. Parts 167 Leak System Parts 168 Upgrade Kits 170 Large Volume Injection Kit multi draw 171 1 Identifying Cables 173 Cable Overview 174 Analog Cables 176 Remote Cables 178 BCD Cables 181 CAN LAN Cables 183 External Contact Cable 184 Agilent Module to PC 185 Agilent 1200 Module to Printer 186 12 Hardware Information 187 Firmware Description 188 Boot up and Initialization Process 191 Electrical Connections 192 Interfaces 194 Setting the 8 bit Configuration Switch without On board LAN 201 Early Maintenance Feedback 205 Instrument Layout 206 13 LAN Configuration 207 Setting up the module in a LAN environment 208 Connecting the module via LAN 209 Agilent 1290 Infinity Autosampler User Manual Contents 14 Appendix 211 General Safety Information 212 Lithium Batteries Information 215 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 216 Radio Interference 217 Sound Emission 218 Agilent Technologies on Internet 219 8 Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 1 Introduction Features 10 Overview of the Module 11 Autosampler Principle 13 System Overview 19 Leak and Waste Handling 19 This chapter gives an introduction to the autosampler ee Agilent Technologies 1 introduction Features 10 The 1290 Infinity Autosampler features an increased pressure range enabling the use of today s column techn
15. accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 192 Agilent 1290 Infinity Autosampler User Manual Rear view of the module Hardware Information Electrical Connections Figure 37 Rear view of the module Agilent 1290 Infinity Autosampler User Manual CO Agilent Technologies Prodect No QUMA Bomian Swa Ne Ofteerte TT tet tangen ET a we Made in Germany 12 193 12 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 10 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN optional on board RS 232 Analog APG Remote Special Pumps G1310B Iso Pump 2 G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump Yes No G4220A B Bin Pump 2 No G4204A Quat Pump Yes G1361A Prep Pump 2 Yes No Samplers G132
16. and minimizes the bench space required The Agilent 1290 Infinity Binary Pump should always be installed at the bottom of the stack Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler Optimizing the Stack Configuration HN WN AUN NAAT Instant Pilot Solvent cabinet Detector Column compartment Autosampler Figure 7 Recommended stack configuration for 1290 Infinity front view Agilent 1290 Infinity Autosampler User Manual 35 3 Installing the Autosampler Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Instant Pilot Analog detector signal optional AC Power CAN Bus cable Figure 8 Recommended stack configuration 1290 Infinity rear view 36 Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler 3 Optimizing the Stack Configuration Two Stack Configuration In case the autosampler thermostat is added to the system a two stack configuration is recommended which places both heavy modules 1290 Infinity pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 9 on page 37 and Figure 10 on page 38 Instant Pilot Detector Column compartment ANIMA UUNIT A
17. assembly Please contact your Agilent service representative 3 Defective transport assembly flex board Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual 109 7 Error Information Initialization Failed Error ID 4020 The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause Suggested actions 1 Side door not installed correctly Check if the side door is installed correctly Check if the magnet is in place in the side door 2 Sample transport sampling unit not aligned Do an auto alignment correctly 3 Mechanical obstruction Ensure unobstructed movement of the transport assembly 4 Defective sampling unit flex board Please contact your Agilent service representative 5 Defective transport assembly flex board Please contact your Agilent service representative 6 Defective sampling unit motor Please contact your Agilent service representative 7 Defective main board Please contact your Agilent service representative 110 Agile
18. assembly if necessary Exchange the needle carrier assembly Clean or change the needle seat assembly if necessary Please contact your Agilent service representative Please contact your Agilent service representative The needle carrier on the Sample Transport Assembly failed to move correctly Probable cause 1 Defective Z motor 2 Vial pusher blocked 3 Bad needle carrier positioning in X or Theta 4 Defective vial pusher sensor 5 Defective main board Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual Error Information 7 Missing Vial or Missing Wash Vial Error ID 4019 4034 4035 4541 4542 4706 4707 No vial was found in the position defined in the method or sequence When the needle carrier moves to a vial and the needle goes into the vial the position of the needle is monitored by an encoder behind the vial pusher If no vial is present the encoder detects an error and the message missing vial is generated Probable cause Suggested actions 1 Novialin the position defined inthe method Install the sample vial in the correct position or or sequence edit the method or sequence accordingly 2 Defective needle carrier
19. can be found in the Tools section Agilent 1290 Infinity Autosampler User Manual 145 9 Maintenance Replacing the Rotor seal Replacing the Rotor seal When When poor injection volume reproducibility or when injection valve is leaking Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2394 Hex key 9 64 inch 15 cm long T handle Parts required p n Description 1 5068 0007 Injection valve rotor seal 1 Open the front door 2 Remove all capillaries from the Injection valve with a 1 4 inch wrench 146 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Replacing the Rotor seal 3 Unscrew and remove the three stator screws from the stator head with a 9 64 inch hex key Se ZA a 4 Remove the stator head and the stator ring Agilent 1290 Infinity Autosampler User Manual 147 9 Maintenance Replacing the Rotor seal 7 Install new rotor seal and isolation seal 8 Reinstall the stator ring and stator head The pins on stator ring and stator head must engage in the corresponding holes 9 Insert and tighten the stator screws alternating with the 9 64 inch hex key until the stator head is secure Ah Next Steps 10 Reconnect all capillaries to the injection valve ports with a 1 4 inch wrench The positions of the individual fittings can be seen on the sticker on the sampling unit 11 Close the front door
20. cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized mod
21. gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment Agilent 1290 Infinity Autosampler User Manual 135 9 Maintenance Overview of Maintenance The following pages describe maintenance simple repairs of the autosampler
22. in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even though this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection The delay volume in a system includes the volume in the pump from the point of mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column 70 Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 How to Configure the Optimum Delay Volume For very fast gradients over 0 5 min which can only be achieved in the Agilent 1290 Infinity Binary LC System the delay volume of the system can be easily reduced without changing the physical configuration of the system This change is achieved by changing the behavior of the autosampler The 80 ul delay volume of the Agilent 1290 Infinity Autosampler is due to the flow path from the injection valve through the metering device needle needle seat and connecting capillaries ba
23. maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets Agilent 1290 Infinity Autosampler User Manual 39 3 Installing the Autosampler Installation Information on Leak and Waste Handling Figure 11 Leak and waste handling overview typical stack configuration as an example 40 Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler 3 1 Solvent cabinet Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the sampler s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 11 on page 40 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect ca
24. may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on
25. of the system When By suspected Autosampler performance problems Preparations Solvents must be present in both channels 1 Run the Autosampler Leak Test with the Agilent Lab Advisor for further information see Online Help of user interface General Limts Signals Test Name ALS leak test for A Description Preliminary ALS leak test for Aladdin Module gt Approx Time Not defined Status Passed COTTI Test Procedure Rest Name Value 1 Checking pre requisites pumo on eic Metering device leak Flush the system Needle seat leak Total sampler leak wn Insert blank nut Measuring leak rates Evaluate results QSSSSR8 nn s Restore system configuration Figure 22 Sampler Leak Test Results 122 Agilent 1290 Infinity Autosampler User Manual Test Functions 8 Sampler Leak Test Evaluation Sampler Leak Test Failed Probable cause Suggested actions 1 Leaky metering device seal Change metering device seal 2 Damaged needle and or needle seat Change needle and needle seat 3 Damaged rotor seal in the injection valve Change Rotor seal 4 Leaky fittings Tighten fittings or replace capillaries Notice the difference between error in the test and a failed result An error is caused by an abnormal termination during the operation of the test whereas a failed result indicates that the test result were not within the specified limits Agilent 1290 Infinity Autosampler User Manual 123
26. operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 218 Agilent 1290 Infinity Autosampler User Manual Appendix 14 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent 1290 Infinity Autosampler User Manual 219 Index Index 8 bit configuration switch without On Board LAN 201 A accessory kit 163 Agilent Lab Advisor software 94 Agilent Lab Advisor 94 Agilent oninternet 219 ambient non operating temperature 27 ambient operating temperature 27 analog signal 198 analog cable 1 6 apgremote 199 arm 128 position 128 automated delay volume reduction 87 bandwidth 82 battery safety information 215 BCD cable 181 bench space 26 C cable analog 1 6 BCD 181 CAN 183 220 external contact 184 LAN 183 remote 1 8 RS 232 185 cables analog 174 BCD 174 CAN 175 external contact 175 LAN 175 overview 174 remote 1 4 RS 232 175 CAN cable 183 carryover 87 cleaning 137 Communication settings RS 232C 202 compensation sensor open 101 compensation sensor short 102 condensation 26 configuration one stack 34 two stack front 37 two stack rear 38 two stack 37 D data collection rate 84 defect on arrival 32 delay volume description 70 delivery checklist 33 33 detector achievi
27. over Injection D needle 2 L d gt 2 gt 2 gt gt To waste Sample loop Metenng device aP fush port Peristaltic pump From pump Injection valve To column Mainpass Position Figure 1 14 Agilent 1290 Infinity Autosampler User Manual Introduction 1 Autosampler Principle When the sample sequence begins the valve unit switches to the bypass position Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figure 2 Bypass Position Agilent 1290 Infinity Autosampler User Manual 15 1 Introduction The standard injection starts with draw sample from vial In order to do this the needle moves to the desired sample position and is lowered into the sample liquid in the sample to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close the sample loop In case of an injector program several steps are interspersed at this point t Sample loop Metering device Injection needle Pp Aush port gt gt gt gt gt gt gt g gt gt D gt gt Poristaltic gt gt pump gt To waste From pump Ingection valve To column Figure 3 Drawing the Sample 16 Agilent 1290 Infinity Autosampler User Manual Introduction 1 Autosampler Principle Flush the Needle Before injection and to re
28. settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 14 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 Table 15 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 16 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 17 Parity Settings without on board LAN Switches Parity 7 e 0 0 No Parity 0 1 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the
29. size and a longer column will give a higher plate number The pressure rises with the inverse square of the particle size and proportionally with the length of the column This is the reason that the 1290 Infinity LC system was designed to go to 1200 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small molecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and that can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be of
30. that can be carried out without opening the main cover Table8 Overview of Maintenance Procedure Typical Frequency Notes Changeneedle needle 60 000 needle into seat seat Change metering seal 30 000 injections Peristaltic pump 3000 hours on time cartridge Change rotor seal 30 000 injections 136 Agilent 1290 Infinity Autosampler User Manual Maintenance Cleaning the module 9 To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path Agilent 1290 Infinity Autosampler User Manual 137 9 Maintenance Removing the needle assembly When When the limit in the needle into seat counter in the EMF is exceeded or when needle shows indications of damage blockage or leaks Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description G4226 87201 Needle assembly Preparations In order to avoid leaks close the shutoff valves in the pump or remove tubings from solvent bottles WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful working at the needle carrier assembly gt Use the silicon
31. the instant of injection The question should be asked whether the gradient has already reached the autosampler in which case a small step in the gradient will result This happens when the delay volume is less than the flush out volume and is not necessarily a problem but may be a factor to be considered in a method transfer With a flush out factor of 5 and an injection volume of 10 ul the autosampler will allow 50 ul to pass through before switching to bypass which with a delay volume of 50 ul means the gradient just reached the injection valve Smaller injection volumes will have no effect but for larger injection volumes this will introduce a small step in the gradient The flow rate in use will also have an impact on the decision to use ADVR or not At 0 2 ml min the delay time saved is 21 seconds while at 1 0 ml min it is 4 seconds The ADVR function is unlikely to be suitable for applications involving compounds which are known to cause carry over problems Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 How to Achieve Higher Injection Volumes The standard configuration of the Agilent 1290 Infinity Autosampler includes a variable volume sample loop for up to 20 ul injections The metering device can inject a maximum volume of 40 ul and the sample loop cartridge can be exchanged to allow this The system delay volume due to the autosampler will increase accordingly To extend the injection range once again
32. the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Table3 Agilent 1290 Infinity Autosampler Description Quantity Autosampler 1 Power Cable 1 User Manual on Documentation CD part ofthe 1 per order shipment not module specific Accessory Kit 1 Autosampler Accessory Kit Contents ltem p n Description 1 G4226 68705 Accessory kit 5067 4659 SS Capillary 340x0 12 ps ns 3 5042 1386 96 well plate 0 5 ml PP pack of 10 4 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5 5181 1516 CAN cable Agilent module to module 0 5 m 6 8710 0510 Wrench open 1 4 5 16 inch Agilent 1290 Infinity Autosampler User Manual 33 3 Installing the Autosampler Optimizing the Stack Configuration 34 If your module is part of a complete Agilent 1290 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume For other possible configurations please refer to the Agilent 1290 Infinity System Manual One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Binary LC System in the following configuration See Figure 7 on page 35 and Figure 8 on page 36 This configuration optimizes the flow path for minimum delay volume
33. you can either use a 1290 Infinity large volume kit G4266 68714 which increases the range of the injection volume up to 100 uL or 120 uL depending which loop size is installed or install the 100 uL Upgrade Kit G4214A which is reducing the pressure limit to 600 bar Whenever a method is scaled down from a larger column to a smaller column it is important that the method translation makes an allowance for reducing the injection volume in proportion to the volume of the column to maintain the performance of the method This is to keep the volume of the injection at the same percentage volume with respect to the column This is particular important if the injection solvent is stronger more eluotropic than the starting mobile phase and any increase will affect the separation particularly for early running peaks low retention factor In some cases it is the cause of peak distortion and the general rule is to keep the injection solvent the same or weaker than the starting gradient composition This has a bearing on whether or by how much the injection volume can be increased and the user should check for signs of increased dispersion wider or more skewed peaks and reduced peak resolution in trying to increase the injection size If an injection is made in a weak solvent then the volume can probably be increased further because the effect will be to concentrate the analyte on the head of the column at the start of the gradient Conversely if the in
34. 0 Infinity Autosampler User Manual 171 10 Parts for Maintenance Large Volume Injection Kit multi draw 172 Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 11 Identifying Cables Cable Overview 174 Analog Cables 176 Remote Cables 178 BCD Cables 181 CAN LAN Cables 183 External Contact Cable 184 Agilent Module to PC 185 Agilent 1200 Module to Printer 186 This chapter provides information on cables used with the 1290 series of HPLC modules ee Agilent Technologies 173 11 Identifying Cables Cable Overview 174 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 178 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose Agilent 1290 Infinity Autosampler User Manual CAN cables p n
35. 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative 100 Agilent 1290 Infinity Autosampler User Manual Error Information 7 Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The cu
36. 067 4659 G1329 43200 n a 2x G4226 43800 Parts for Maintenance 10 Recommended Vial Plates Description Vial plate for 54 x 2 mL vials 6 pk Vial plate for 15 x 6 mL vials 1 pk Vial plate for 27 Eppendorf tubes 1 pk Description Accessory kit CAN cable Agilent module to module 1 m Screw Cap Vial 2 mL amber glass write on spot 100 pk Blue screw caps 100 pk Wrench open 1 4 5 16 inch Rheotool socket wrench inch Hex key 4mm15 cm long T handle Hex key 9 64 inch 15 cm long T handle Hex key 3 mm12 cm long Tubing 1 mm i d 3 mm o d silicone 5 m SS Capillary 340x0 12 ps ns Adapter air channel Tubing clips Seal insert tool Agilent 1290 Infinity Autosampler User Manual 163 10 Parts for Maintenance Analytical Head Assembly Analytical Head Assembly gt Figure 32 Analytical Head Assembly 164 Agilent 1290 Infinity Autosampler User Manual Parts for Maintenance 10 Item p n Description G4226 60013 40 uL analytical head 1 0515 0850 Screws 5064 8293 Micro Plunger assembly 3 G1377 60012 Micro seal support 4 0905 1717 Metering seal 5 G4226 27701 Head body 6 G4226 60301 Metering capillary SST Cap 0 17 mmi d 160 mm pre swaged not shown Agilent 1290 Infinity Autosampler User Manual 165 10 Parts for Maintenance Injection Valve Assembly Injection Valve Assembly Item p n Description 1 5067 4114 Injection valve actuator 2 1535 4045 Isolation seal 3 5068 0118 Stator ring 4 50
37. 10 l ogge Draw Speed 100 0 l min Eject Speed _ 1100 0 ulmin _ Exit oler with Agilent Instant Pilot Ry 14 11 l The Configure screen allows to configure Symbolic Name of module Volumes On Missing Vessel behaviour e Plate configuration Flush Out Pump Serial Interface configuration Sample Illumination The Method screen lists all method parameters of the autosampler These can be edited Agilent 1290 Infinity Autosampler User Manual Using the Module Main Screens of the Autosampler with Agilent Instant Pilot G4208A The Maintenance screen allows EMF setup logging of maintenance activities module identification blinking LED Firmware updates can be done via the System Maintenance screen Maintenance HiP ALS System Controller Bin Pump HiP ALS The Diagnosis screen provides access to module specific tests Pressure Test Injector steps Injector Steps Lamp intensity test Calibration Test Cell test No Pass Fail result Agilent 1290 Infinity Autosampler User Manual 4 63 4 Using the Module Solvent Information 64 Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see pump manuals e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid or minimize the use of solvents that
38. 13 oe Agilent Technologies 95 7 Error Information Vessel or Wash Vessel Error 114 Vessel Stuck to Needle 114 Rear Blind Seat Missing 115 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 96 Agilent 1290 Infinity Autosampler User Manual Error Information 7 What are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in th
39. 290 Infinity Autosampler User Manual Hardware Information 12 Setting the 8 bit Configuration Switch without On board LAN The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module i SS Y Figure 39 Configuration switch settings depend on configured mode All modules without on board LAN default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Agilent 1290 Infinity Autosampler User Manual 201 12 Hardware Information 202 The following tables represent the configuration switch settings for the modules without on board LAN only Table 13 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN
40. 4 uL The Max Light high sensitivity cell has a path length of 60 mm and this will give between three and five times increase in signal to noise values depending on the application conditions The dispersion volume is fractionally increased compared to the standard cell Wavelength and Bandwidth The detector measures absorbance simultaneously at wavelengths from 190 nm to 640 nm using diode array detection A UV lamp provides good sensitivity over the whole wavelength range The diode array detector DAD can simultaneously compute and send to the data system up to eight chromatographic signals and the full range spectra at every time point A UV chromatogram or signal is a plot of absorbance data versus time and is defined by its wavelength and bandwidth e The wavelength indicates the center of the detection band The bandwidth defines the wavelength range over which the absorbance values are averaged to give the result at each time point Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 For example a signal at wavelength 250 nm with a bandwidth of 16 nm will be an average of the absorbance data from 242 nm to 258 nm Additionally a reference wavelength and reference bandwidth can be defined for each signal The average absorbance from the reference bandwidth centered on the reference wavelength will be subtracted from its equivalent value at the signal wavelength to produce the output chromatogram Th
41. 68 0007 Injection valve rotor seal 5 5068 0006 Stator head 6 5068 0018 Stator screws 166 Agilent 1290 Infinity Autosampler User Manual Parts for Maintenance 10 Cover Parts Cover Parts Figure 33 Cover parts Item p n Description 1 5067 4662 Cabinet kit base sides and top 5042 9964 Name plate for Agilent 1290 series G4226 67001 Door repair kit includes the front door Agilent 1290 Infinity Autosampler User Manual 167 10 Parts for Maintenance Leak System Parts Leak System Parts Figure 34 Leak system parts 168 Agilent 1290 Infinity Autosampler User Manual Parts for Maintenance 10 Leak System Parts Item p n Description 1 5061 3356 Leak sensor 2 G4226 44511 Leak plane 3 0890 1711 Leak tubing 185 mm 4 5041 8388 Leak funnel Agilent 1290 Infinity Autosampler User Manual 169 10 Parts for Maintenance Upgrade Kits p n 5067 4703 G4214A 170 Description 40 uL Flex loop kit 100 uL Injection Kit includes 100 uL Flex Loop Kit 5067 4710 and analytical head G1367 60003 only for 600 bar Agilent 1290 Infinity Autosampler User Manual Parts for Maintenance 10 Large Volume Injection Kit multi draw Figure 35 Extension Seat Capillary 80 uL Item p n Description 1 G4216 68711 Large Volume Injection Kit multi draw contains the following 2 items 2 G4216 90000 1290 Infinity 1200 bar Multi draw Tech Note ENG 3 G4226 87303 Extension Seat Capillary 80 pL 0 5 mm ID 0 9 mm OD Agilent 129
42. 90 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 9 Maintenance Introduction to Maintenance 134 Warnings and Cautions 135 Overview of Maintenance 136 Cleaning the module 137 Removing the needle assembly 138 Installing the needle assembly 141 Exchanging the Needle Seat 144 Replacing the Rotorseal 146 Removing the Metering Seal 149 Installing the Metering Seal 152 Replacing Peristaltic Pump Cartridge 154 Installing the Interface Board 157 Replacing the Module Firmware 158 This chapter describes the maintenance of the Autosampler ae Agilent Technologies 133 9 Maintenance Introduction to Maintenance Introduction to Maintenance Figure 31 on page 134 shows the main user accessible assemblies of the autosampler These parts can be accessed from the front simple repairs and don t require to remove the autosampler from the system stack Metering device Loop capillary Transport assembly Needle carrier Needle Injection valve Needle wash peristaltic pump Figure 31 Main user accessible assemblies 134 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Warnings and Cautions Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety
43. 9B ALS 2 G2260A Prep ALS Yes No G1364B FC PS 2 G1364C FC AS G1364D FC uS G1367E HiP ALS K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler Yes No G4226A ALS 2 Yes No Yes 1 Yes Yes No CAN DC OUT for CAN slaves Yes Yes No CAN DC OUT for CAN slaves Yes Yes No THERMOSTAT for G1330B K1330B Yes Yes No THERMOSTAT for G1330B K1330B CAN DC OUT for CAN slaves Yes Yes No Yes 194 Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 Table 10 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed Agilent 1290 Infinity Autosampler User Manual 195 12 Hardware Informatio
44. Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 7 Error Information What are Error Messages 97 General Error Messages 98 Timeout 98 Shutdown 98 Remote Timeout 99 Lost CAN Partner 100 Leak Sensor Short 100 Leak Sensor Open 101 Compensation Sensor Open 101 Compensation Sensor Short 102 Fan Failed 102 Leak 103 Module Error Messages 104 Exhaust Fan Failed 104 Front Door Error 104 Side Door Error 105 Arm Movement Failed or Arm Movement Timeout 105 Valve to Bypass Failed 106 Valve to Mainpass Failed 106 Needle Lock Failed 107 Needle to Needle Seat Position 108 Needle Carrier Failed 108 Missing Vial or Missing Wash Vial 109 Initialization Failed 110 Metering Home Failed 111 Motor Temperature 112 Invalid Vial Position 113 Peristaltic Pump Error 1
45. Agilent ChemStation B 04 02 and above do not include any maintenance test functions e Screenshots used within these procedures are based on the Agilent Lab Advisor Software Agilent 1290 Infinity Autosampler User Manual 93 Troubleshooting and Diagnostics Agilent Lab Advisor Software 94 The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status Early Maintenance Feedback counters EMF instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon request the user can send this report to Agilent for a significantly improved troubleshooting and repair process The Agilent Lab Advisor software is available in two versions e Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor
46. Contact Cable c50 o o ol 10 6 15 41 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 184 Agilent 1290 Infinity Autosampler User Manual Agilent Module to PC p n G1530 60600 RS232 61601 5181 1561 Identifying Cables 11 Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1290 Infinity Autosampler User Manual 185 11 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 186 Agilent 1290 Infinity Autosampl
47. DB9 DB9 Male Female Female Male Figure 38 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e ou
48. FW Update Figure 36 Firmware Update Mechanism Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 190 Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 Boot up and Initialization Process CAUTION Obstruction of transport unit Any obstruction of the transport unit during the initialization process will result in a wrong transmission ratio and thus wrong needle positions gt Make sure no vials or other material gets into the X slide 1 Firmware Boot Process a Start Boot Loader b Boot main firmware OR Boot resident firmware if set in VRAM by DIP switch or if no wrong main FW is found 2 Initialize Transport Unit a Switch injection valve to bypass positio
49. Failed Probable cause Suggested actions 1 Pump leakages Perform the Pump Head Leak test 2 Loose or leaky fittings Tighten the fittings or replace capillaries 3 Autosampler leakages Perform the Autosampler Leak test 4 Thermostatted Column Compartment valve Replace the TCC valve rotor seal leakages e Notice the difference between error in the test and a failed result An error is caused by an abnormal termination during the operation of the test whereas a failed result indicates that the test results were not within the specified limits e Often it is only a damaged blank nut poorly shaped from over tightening that causes the test to fail Before investigating any other possible sources of failure make sure that the blank nut you are using is in a good condition and properly tightened Agilent 1290 Infinity Autosampler User Manual 121 8 Test Functions Sampler Leak Test The test determines the specific leak rates of rotor seal metering device needle seat and system by performing a series of pressure tests This is done with the injection valve in different positions and by using a blocked needle seat positioned at the rear of the module to block certain parts of the flow path The test allows for setting the pressure at which the test is performed The leak rate of high pressure parts are not always a linear function and therefore it is recommended to perform the test at a pressure that correspond to the normal operating pressure
50. LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Agilent 1290 Infinity Autosampler User Manual 197 12 Hardware Information 198 Table 11 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9
51. Maintenance This chapter describes the maintenance of the Autosampler 10 Parts for Maintenance This chapter provides information on parts material required for the module 11 Identifying Cables This chapter provides information on cables used with the 1290 series of HPLC modules 12 Hardware Information This chapter describes the autosampler in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connecting the autosampler to the Agilent ChemStation PC 14 Appendix This chapter provides addition information on safety legal and web Agilent 1290 Infinity Autosampler User Manual Contents Contents 1 Introduction 9 Features 10 Overview of the Module 11 Autosampler Principle 13 System Overview 19 2 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 3 Installing the Autosampler 31 Unpacking the Autosampler 32 Optimizing the Stack Configuration 34 Installation Information on Leak and Waste Handling 39 Installing the Autosampler 43 Flow Connections to the Autosampler 45 4 Usingthe Module 47 Leak and Waste Handling 48 Preparing the Autosampler 49 Setting up the Autosampler with Agilent ChemStation 51 Main Screens of the Autosampler with Agilent Instant Pilot G4208A 61 Solvent Information 64 5 Optimizing Performance 69 Delay Volume and Extra Column Volume 70 How to Configure th
52. Test Functions 8 Change Metering Device When removing the metering device is necessary by exchanging the metering seal for instance the metering drive needs to be moved to a position at the far back in order to prevent seal and or piston damage Change Metering Device Figure 29 Maintenance Positions Change Metering device Agilent 1290 Infinity Autosampler User Manual 129 8 Test Functions Injector Steps Injector Steps Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions enabling the specific step to be done When When troubleshooting the module 1 Run the Injector steps with the Agilent Lab Advisor for further information see Online Help of user interface Agilent Lab Advisor Agilent Lab Advisor ox Agilent Lab Advisor Version B 1 02 09096 2300 Current Instrument 1290 Infinity LC DE00000000 Current User AGILENT je1208 Administrator Tools Valve Bypass Step Status Plunger Home Needle Up Location PLAT Move To Location Needle Into Sample Draw Needle Up Needle Into Seat G42264 High Performar Serial LPOC Valve Mainpa
53. act your Agilent service misplaced broken representative 2 The sensor on the main board is defective Please contact your Agilent service representative Arm Movement Failed or Arm Movement Timeout Error ID 4002 The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated Axes identification e Arm Movement 0 Failed X axis e Arm Movement 1 Failed Z axis e Arm Movement 2 Failed Theta needle carrier rotation Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Defective motor assembly Please contact your Agilent service representative 4 Defective sample transport assembly Please contact your Agilent service representative flex board 5 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual 105 7 Error Information Valve to Bypass Failed Error ID 4014 4701 The injection valve failed to switch to the bypass position T
54. ampler tray 61364A B C D Test Procedure 1 Initialize sampler for the calibration 2 Verify that an appropriate well plate tray got installed 3 Ensure that the tray is empty 4 Ensure that the door is closed 5 Align the transport unit and the well plate tray Figure 23 Sample Transport Self Alignment Running Agilent 1290 Infinity Autosampler User Manual 125 8 Test Functions Maintenance Positions Some maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the Agilent Lab Advisor Software the maintenance positions can be selected from the Tools icon When When performing Maintenance on the module 1 Run the Maintenance Positions with the Agilent Lab Advisor for further information see Online Help of user interface amp Agilent Lab Advisor Agilent Lab Advisor i Agilent Lab Advisor 4 ersion B 1 02 09096 2300 Current User AGILENT je1208 Administrator Current Instrument 1290 Infinity LC DE00000000 Tools Change Needle Change Loop Capillary Arm Position a Start Start Move Arm Home Instrument Change Needle Carrier Change Metering Device Stat Start Help 1120 Tools overview 1200 Tools overview Figure 24 Maintenance Positions Running 126 Agilent 1290 In
55. anual Agilent 1290 Infinity Autosampler User Manual e Installing the Autosampler J Unpacking the Autosampler Damaged Packaging 32 Delivery Checklist 33 Optimizing the Stack Configuration One Stack Configuration Two Stack Configuration 32 34 34 37 Installation Information on Leak and Waste Handling 39 Installing the Autosampler 43 Flow Connections to the Autosampler 45 This chapter provides information on unpacking checking on completeness stack considerations and installation of the autosampler ee Agilent Technologies 31 3 Installing the Autosampler Unpacking the Autosampler Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 32 Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler 3 Delivery Checklist Ensure all parts and materials have been delivered with the autosampler For this compare
56. ation Instrument 1 online Method amp Run Control File RunControl Instrument Method Sequence View Abort Help a f i Methods 2 lid DeF_Lc m gt Sequences R eh DEF_LC S Aaa Brg4d GaP C CHEMS2 1 SEQUENCE i AFCDELAY S Gi BATCH S SL DEF_LC S F DGNOISE S iy INSTPERF S Gy LOADTEST S Sg ROBUST S ZE ROUTINE S I SEQDOC S F SEQSUM S Sy STATIST S 52 Method and Run Control Esl Data Analysis E4 Report Layout 2 Verification 09 Pv Main Automation Controls Single Sample 4 i N x OE Injection Volume _ Sample Location npi24019 DEF LCM 1290 Infinity Autosampler Idie ME TT 0 0 uL Instrument 0 00 0 00 min Idle t Data Dir DEFAULT D C data Data Analysis Figure 14 ChemStation Method amp Run Control Agilent 1290 Infinity Autosampler User Manual T instrument 1 LJ Read Setting up the Autosampler w Using the Module 4 ith Agilent ChemStation The Autosampler User Interface Injection Volume 0 0 ul Sample Location Set Error Method Identify Device Injection Volume needle up Sample Location TST Switch Valve to Bypass Switch on Tray Illumination npi24019 Edit Wellplate Types Vru lt gt Assign Wellplates Agilent 1290 Infinity Autosampler User Manual Within the Autosampler user interface there are active areas If you move the mouse cursor a
57. cal and Electronic Equipment WEEE Directive 2002 96 EC 216 Radio Interference 217 Sound Emission 218 Agilent Technologies on Internet 219 This chapter provides addition information on safety legal and web ee Agilent Technologies 211 14 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 212 Agilent 1290 Infinity Autosampler User Manual Appendix 14 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension
58. cated above and behind the needle seat and a peristaltic pump delivers the wash solvent It has a volume of 0 68 ml and the peristaltic pump delivers 6 ml min which means the flush port volume is completely refilled with fresh solvent in 7 s If the flush port is selected the user can set how long the outside of the needle is to be washed with fresh solvent This may be as low as two or three seconds in routine situations where carryover is less of a problem and 10 to 20 s for more complete washing It is recommended that washing the outside of the needle in the flush port should be standard procedure to avoid contaminating the needle seat If the needle seat becomes contaminated it will have to be back flushed by manually changing the flow connections to clean it This is one of the tasks that can be automated using the Flexible Cube module The flush port and its solvent delivery pump and tubing should be regularly flushed to ensure the lowest carryover For example before using the system each day prime the flush pump for three minutes with appropriate solvent When other measures have failed to eliminate carryover it might be that analyte is sticking inside the injector valve The injector valve can be set to make additional switching movements to clean out the flow path in the valve if problems occur here with carryover If the problem compounds need a high percentage of organic phase for elution it is recommended to switch the injection
59. ce 10 Vial Trays Description Tray for 2 plates 10 x 2 mL vials Screws for springs Spring Spring stud Tray Support Adapter air channel Plug channel Tray for 100 micro vials 161 10 Parts for Maintenance Recommended Plates and Closing Mats Table9 Recommended plates and closing mat Description Part Number Rows Columns Plate height Volume uL Package 384 Agilent 5042 1388 16 24 14 4 80 30 384Corning No Agilent PN 16 24 14 4 80 384Nunc No Agilent PN 16 24 14 4 80 96 well plate 0 5 ml PP pack of 10 8 12 14 3 500 10 5042 1386 120 96 well plate 0 5 ml PP pack of 120 5042 1385 96Agilent conical 5042 8502 8 12 17 3 150 25 96CappedAgilent 5065 4402 8 12 47 1 300 1 96Corning No Agilent PN 8 12 14 3 300 96CorningV No Agilent PN 8 12 14 3 300 96DeepAgilent31mm 5042 6454 8 12 31 5 1000 50 96DeepNunc31mm No Agilent PN 8 12 31 5 1000 96DeepRitter41mm No Agilent PN 8 12 41 2 800 96Greiner No Agilent PN 8 12 14 3 300 96GreinerV No Agilent PN 8 12 14 3 250 96Nunc No Agilent PN 8 12 14 3 400 Closing mat for all 96 Agilent plates 8 12 50 5042 1389 Using vessels higher than 41 mm will result in needle not being able to reach bottom of vessel 162 Agilent 1290 Infinity Autosampler User Manual p n G2255 68700 5022 6539 5022 6538 Accessory Kit p n G4226 68705 5181 1519 5182 0716 5182 0717 8710 0510 2x 8710 2391 8710 2392 8710 2394 8710 2411 5065 9978 5
60. chemical compatibility of materials Agilent 1290 Infinity Autosampler User Manual Using the Module 4 PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in a pH range between 1 12 and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity p
61. ck to the injection valve see Figure 16 on page 72 To make an injection the valve switches from mainpass to bypass so that the metering device can draw the sample into the needle capillary The injection is made when the valve switches back to mainpass and the sample is flushed onto the column The valve remains in this position during analysis so that the autosampler is continually flushed and hence the gradient has to flow through this delay volume to reach the column This can be eliminated by switching the injection valve from mainpass to bypass after the injection has been made and the injected sample has been flushed onto the column In practice this can be done a few seconds after injection and is activated by selecting the Automatic Delay Volume Reduction ADVR function in the autosampler setup menu The Flush out Factor typically 5 times injection volume ensures that enough time is allowed to flush the sample out of the injector before switching to bypass This effectively reduces the system delay volume from 125 ul to 50 ul Agilent 1290 Infinity Autosampler User Manual 71 5 72 Optimizing Performance 1 Valve in mainpass flow through 2 Valve in bypass drawing sample rf d i tee 3 Valve in bypass washing needle 4 Valve in mainpass sample injected Figure 16 Schematic of injection steps in 1290 Infinity Autosampler When using ADVR it should be noted that the gradient has already started at the pump at
62. cross the icons tray EMF button the cursor will change and you may click on the icon to Turn on off the autosampler 1 Configure the sample tray 2 Get the status of the EMF Early Maintenance Feature 3 Switch injection valve to Mainpass Bypass 4 Instrument actuals Information Injection volume Sample location A right click into the Active Area will open a menu to Show the Control User Interface special module settings Show the Method User interface same as via menu Instrument Setup G4226A Set Error Method Identify Device Home Arm Reset Sampler Wash Needle Needle Up Valve Mainpass Bypass same as click on the valve icon Switch on Tray Illumination Edit Well Plate Types e Wellplate Configuration same as click on the Tray icon 53 4 Using the Module Setting up the Autosampler with Agilent ChemStation iS 1290 Infinity Autosampler Injection Volume 0 0 ul Sample Location 0 0uL Injection Volume 0 0 uL _ Sample Location 54 Module Status shows Run Ready Error state and Not Ready text or Error text Error Red Not ready yellow Ready green Pre run Post run purple Run blue Idle green Offline dark gray Standby light gray EMF Status shows Run Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Mai
63. densate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container Agilent 1290 Infinity Autosampler User Manual 21 1 22 Introduction System Overview Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 24 Physical Specifications 27 Performance Specifications 28 This chapter provides information on environmental requirements physical and performance specifications ee Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the module Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 27 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord
64. dopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 s This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex l this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Agilent 1290 Infinity Autosampler User Manual Appendix 14 Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1290 Infinity Autosampler User Manual 217 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the
65. duce the carry over for very sensitive analysis the outside of the needle can be washed in a flush port located behind the injector port on the sampling unit As soon as the needle is on the flush port a peristaltic pump delivers some solvent during a defined time to clean the outside of the needle At the end of this process the needle returns to the injection port Figure 4 Flush the needle Agilent 1290 Infinity Autosampler User Manual 17 1 18 Introduction Inject and Run The final step is the inject and run step The six port valve is switched to the main pass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports the sample onto the column and separation begins This is the beginning of a run within an analysis In this stage all major performance influencing hardware is flushed internally by the solvent flow For standard applications no additional flushing procedure is required Sample loop Metering device injection Flush S needle port lt 2 lt gt 2 lt gt gt gt gt gt gt gt Peristaltic pump To waste From pump Injection a To column Figure 5 Inject and Run Agilent 1290 Infinity Autosampler User Manual Introduction System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understo
66. e Optimum Delay Volume 71 How to Achieve Higher Injection Volumes 73 How to Achieve High Throughput 75 How to Achieve Higher Resolution 76 How to Achieve Higher Sensitivity 79 How to Achieve Lowest Carry Over 87 Agilent 1290 Infinity Autosampler User Manual Contents Troubleshooting and Diagnostics 89 Overview of the Module s Indicators and Test Functions 90 Status Indicators 91 User Interfaces 93 Agilent Lab Advisor Software 94 Error Information 95 What are Error Messages 97 General Error Messages 98 Module Error Messages 104 Test Functions 117 Introduction 118 System Pressure Test 119 Sampler Leak Test 122 Sample Transport Self Alignment 124 Maintenance Positions 126 Injector Steps 130 Maintenance 133 Introduction to Maintenance 134 Warnings and Cautions 135 Overview of Maintenance 136 Cleaning the module 137 Removing the needle assembly 138 Installing the needle assembly 141 Exchanging the Needle Seat 144 Replacing the Rotor seal 146 Removing the Metering Seal 149 Installing the Metering Seal 152 Replacing Peristaltic Pump Cartridge 154 Installing the Interface Board 157 Replacing the Module Firmware 158 Agilent 1290 Infinity Autosampler User Manual Contents 10 Parts for Maintenance 159 Overview of Maintenance Parts 160 Vial Trays 161 Recommended Plates and Closing Mats 162 Recommended Vial Plates 163 Accessory Kit 163 Analytical Head Assembly 164 Injection Valve Assembly 166 Cover
67. e for UV 240 ng pL Chlorhexidine for MS dissolved with mobile phase A 1 pL injected and measured both on Agilent 6410 000 and G4212A DAD e Wash solution H20 with 0 1 TFA 5s Control and data Agilent ChemStation for LC B 04 02 or above evaluation EZChrom Elite 3 3 3 or above Mass hunter B 02 01 sp1 or above Lab Advisor B 01 03 or above Local Control Agilent Instant Pilot G4208A B 02 08 or above Communications Controller area network CAN RS 232C APG Remote ready start stop and shut down signals optional four external contact closures and BCD vial number output Agilent 1290 Infinity Autosampler User Manual 29 2 30 Site Requirements and Specifications Table 2 Performance specifications G4226A Type Specification Comment Safety and maintenance GLP features Housing Metering device Extensive diagnostics can be done with the help of the Control Module and Agilent LabAdvisor Diagnostic Software error detection and display through Instant Pilot and Diagnostic Software leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable Metering pump in high pressure flow path Agilent 1290 Infinity Autosampler User M
68. e signal wavelength and bandwidth can be chosen so that they are optimized for Broad band universal detection Narrow band selective detection e Sensitivity for a specific analyte Broad band or universal detection works by having a wide bandwidth to detect any species with absorbance in that range For example to detect all absorbing molecules between 200 nm and 300 nm set a signal at 250 nm with a bandwidth of 100 nm The disadvantage is that sensitivity will not be optimal for any one of those molecules Narrow band or selective detection is used most often The UV spectrum for a particular molecule is examined and an appropriate absorbance maximum is selected If possible the range where solvents absorb strongly should be avoided below 220 nm for methanol below 210 nm for acetonitrile For example in Figure 17 on page 83 anisic acid has a suitable absorbance maximum at 252 nm A narrow bandwidth of 4 nm to 12 nm generally gives good sensitivity and is specific for absorbance in a narrow range The narrow band can be optimized for sensitivity for a specific molecule As the bandwidth is increased the signal is reduced but so is the noise and there will be an optimum for best S N As an approximate guide this optimum is often close to the natural bandwidth at half height of the absorption band in the UV spectrum In the anisic acid example this is 30 nm The analytical wavelength is usually set at a wavelength maximum to increa
69. e specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 188 Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using the following tools latest version should be used Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files on a USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Agilent 1290 Infinity Autosampler User Manual 189 12 Hardware Information Main FW update Resident System Main System Resident
70. e user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1290 Infinity Autosampler User Manual 97 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 98 Agilent 1290 Infinity Autosampler User Manual
71. egatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide Zr0 Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Agilent 1290 Infinity Autosampler User Manual 67 4 68 Using the Module Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and no
72. enerate higher peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008 Maximizing peak capacity and separation speed in liquid chromatography Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 How to Achieve Higher Sensitivity The sensitivity of a separation method is linked to the choice of stationary and mobile phases as good separation with narrow peaks and a stable baseline with minimal noise are desirable The choice of instrument configuration will have an effect and a major impact is the setup of the detector This section considers how sensitivity is affected by e Pump mixer volume Narrower columns Detector flow cell e Detector parameters In addition the discussion on detector parameters also mentions the related topics of selectivity and linearity Columns Sensitivity is specified as a signal to noise ratio S N and hence the need to maximize peak height and minimize baseline noise Any reduction in peak dispersion will help to maintain peak height and so extra column volume should be minimized by use of short narrow internal diameter connection capillaries and correctly installed fittings Using smaller inner diameter columns should result in higher peak height and is therefore ideal for applications with limited sample amounts If the same sample amount can be injected on a smaller i d column then the dilution due t
73. ents faster flow Resolution between two peaks is described by the resolution equation 1 a 1 k Rs VN 2 i 4 a k where R resolution e N plate count measure of column efficiency e o selectivity between two peaks e ko retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selectivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an automated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle
74. er User Manual Agilent 1290 Infinity Autosampler User Manual 12 Hardware Information Firmware Description 188 Boot up and Initialization Process 191 Electrical Connections 192 Rear view of the module 193 Interfaces 194 Overview Interfaces 197 Setting the 8 bit Configuration Switch without On board LAN 201 Communication Settings for RS 232C 202 Special Settings 204 Early Maintenance Feedback 205 Instrument Layout 206 This chapter describes the autosampler in more detail on hardware and electronics Ee Agilent Technologies 187 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote e error handling diagnostic functions or modul
75. et gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F 48 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For details on correct installation see section Installation Information on Leak and Waste Handling in the service manual Agilent 1290 Infinity Autosampler User Manual Using the Module 4 Preparing the Autosampler For best performance of the autosampler e When using the Autosampler in a system with a vacuum degassing unit shortly degas your samples before using them in the autosampler Filter samples before use in 1290 system Use the high pressure filter kit High pressure filter kit 5067 4638 for inline filtering When using buffer solutions flush the system with water before switching it off e Check the autosampler plungers for scratches grooves and dents when changing the piston seal Damaged plungers cause micro leaks and will decrease the lifetime of the seal Solvent Information Observe recommendations on the use of solvents Always filter solvents through 0 4 um filters Small particles can permanentl
76. failed to move successfully The upper and lower positions of the needle lock are monitored by position sensors on the sampling unit flex board The sensors detect the successful completion of the needle lock movement If the needle lock fails to reach the end point or if the sensors fail to recognize the needle lock movement the error message is generated Probable cause Suggested actions 1 Defective or dirty position sensor Clean the position sensor 2 Sticking spindle assembly Please contact your Agilent service representative 3 Defective needle drive motor Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual 107 7 108 Error Information Needle to Needle Seat Position Error ID 4510 4511 4714 The needle failed to reach the end position in the needle seat The position of the needle is monitored by a position encoder on the needle carrier If the needle fails to reach the end point or if the encoder fails to recognize the needle carrier movement the error message is generated Probable cause 1 Bad sample transport sampling unit alignment Bent needle Missing needle gt Ww N Blocked seat 5 Defective position sensor in the needle carrier assembly 6 Defective main board Needle Carrier Failed Suggested actions Do an auto alignment Check and exchange the needle
77. finity Autosampler User Manual Test Functions 8 Change Needle The position is positioning the needle carrier so that there is easy access for changing needle or needle seat The position is to the far left and the current to the motors are off so that the arm can be turned while servicing the module Change Needle Figure 25 Maintenance Positions Change Needle Change Loop Capillary The Change Loop Capillary command positions the arm in the middle of the tray at half height to enable easy exchange of the loop cartridge Change Loop Capillary Figure 26 Maintenance Positions Change Loop Capillary Agilent 1290 Infinity Autosampler User Manual 127 8 Test Functions Arm Position The home position of the autosampler ensures a better access to the tray area and for exchanging trays When transporting the module it is highly recommended to use the Park Arm command in order to place the Arm in a position for safe transport Arm Position Park Arm Figure 27 Maintenance Positions Arm Position Change Needle Carrier The Change Needle Carrier function moves the needle to the front of the autosampler enabling easy access to the needle carrier mechanism Change Needle Carrier Figure 28 Maintenance Positions Needle Carrier Start moves the needle to the front of the sample tray area e End resets the autosampler after the needle carrier has been changed 128 Agilent 1290 Infinity Autosampler User Manual
78. formance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1290 Infinity Autosampler User Manual 205 12 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate
79. fset by reducing the temperature of the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve shows that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns Agilent 1290 Infinity Autosampler User Manual 77 5 78 Optimizing Performance In isocratic separations increasing the retention factor k results in better resolution because the solute is retained longer In gradient separations the retention is described by k in the following equation te F100 A B V S m where k mean k value tg time length of gradient or segment of gradient min F flow ml min Vi column delay volume e A B change in fraction of solvent B during the gradient e S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constant and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can g
80. he coding surfaces of the sample or defective transport assembly flex board tray are clean located at the rear of the Peristaltic Pump Error Error ID 4514 sample tray e Please contact your Agilent service representative The peristaltic pump motor in the autosampler has failed The current on the motor is used by the MTP board to monitor the speed of the peristaltic pump motor If the current falls below a certain value the error message is generated Probable cause 1 Defective motor 2 Defective SUD board 3 Defective main board Agilent 1290 Infinity Autosampler User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 113 7 114 Error Information Vessel or Wash Vessel Error Error ID 4540 4544 4545 4705 4712 The needle does not reach the target position in the vial or in the vessel of the well plate The sensor behind the vial pusher in the needle carrier assembly detects the successful completion of the needle movement to the vessel If the needle fails to reach the end point the sensor fails to recognize the needle movement and the error message is generated Probable cause 1 Bad vessel definition in the plate configuration 2 Closing mat to rigid thick 3 Bad X or Theta positioning 4 Defective encoder on the needle carrier assembly Vessel S
81. he switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the Autosampler main power OFF and ON the bypass and mainpass positions 2 Defective injection valve Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Valve to Mainpass Failed Error ID 4015 The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the Autosampler main power OFF and ON the bypass and mainpass positions 2 Defective injection valve Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 106 Agilent 1290 Infinity Autosampler User Manual Error Information 7 Needle Lock Failed Error ID 4702 4703 The lock assembly on the sampling unit
82. hed under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like
83. ification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1290 Infinity Autosampler User Manual 213 14 Appendix Safety Symbols Table 20 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal CAN Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product e The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up gt WARNING aia alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION aler
84. in board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual Error Information 7 Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak
85. is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 24 Agilent 1290 Infinity Autosampler User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by
86. is a replaceable unit The tubing inside the pump is not replaceable 1 Remove the corrugated leak tubing Press the two clips on the front of the peristaltic pump cartridge 154 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Replacing Peristaltic Pump Cartridge 3 Pull the cartridge forward off the motor shaft 4 Disconnect the tubing leading to the wash port and the tubing coming from the solvent bottle 5 Connect the wash port tubing to the upper tubing of the 6 Connect the tubing coming from the solvent bottle to the new cartridge use sand paper to get a good grip on the lower tubing of the new cartridge tubing Agilent 1290 Infinity Autosampler User Manual 155 9 Maintenance Replacing Peristaltic Pump Cartridge 7 Push the cartridge onto the motor shaft until the clips 8 Reinstall the corrugated leak tubing click into place 156 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Installing the Interface Board When At installation or when defective Tools required Description Flat head screwdriver Parts required Description 1 Interface board CAUTION Electronic boards are sensitive to electrostatic discharge ESD and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD can damage electronic boards and components gt Be sure
87. it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 204 Then try a firmware update see Replacing the Module Firmware on page 158 If this does not help a main board replacement is required Agilent 1290 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 User Interfaces Depending on the user interface the available tests and the screens reports may vary e Preferred tool should be Agilent Lab Advisor Software see Agilent Lab Advisor Software on page 94 e The
88. jection is in a stronger solvent than the starting mobile phase then increased injection volume will spread the band of analyte down the column ahead of the gradient resulting in peak dispersion and loss of resolution Agilent 1290 Infinity Autosampler User Manual 73 5 Optimizing Performance Perhaps the main consideration in determining injection volume is the diameter of the column as this will have a big impact on peak dispersion Peak heights can be higher on a narrow column than with a larger injection on a wider column because there is less peak dispersion With 2 1 mm i d columns typical injection volumes might range up to 5 tol0 ul but it is very dependent on the chemistry of the analyte and mobile phase as discussed above In a gradient separation injection volumes of about 5 of the column volume might be achieved whilst maintaining good resolution and peak dispersion One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the injection before switching it i e injecting it onto an analytical column see Sample Enrichment on page 74 The valve can be conveniently located in the thermostatted column compartment or in the flexible cube Sample Enrichment Pump A autosampler Pump A autosampler Waste Position 1 74 Analytical Analytical column A detector sou detector Enrichment column Enrichment column Position 2 Agilent 1290 I
89. l Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Agilent 1290 Infinity Autosampler User Manual 181 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 182 Agilent 1290 Infinity Autosampler User Manual CAN LAN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Agilent 1290 Infinity Autosampler User Manual 183 11 Identifying Cables External
90. ll effectively fall onto a small band of diodes the width of which is proportional to the width of the entrance slit The description of the slit as 4 nm describes this behavior the light falls on the number of diodes that detect a bandwidth of 4 nm It follows that the minimum optical resolution will be 4 nm and therefore the diode array or digital bandwidth should be set to 4 nm or greater For optimum sensitivity the 8 nm setting will allow most light in and will minimize noise but spectral resolution is at its lowest This is not usually a problem with UV spectra as their natural bandwidths are usually greater than 25 nm without any fine structure The optical bandwidth at 8 nm reduces the linearity range compared to 4 nm slit so it is important that a validated method always uses the slit width that was used for validation For optimum spectral resolution the lnm setting is best This will enable fine structure such as in the benzene spectrum to be resolved see Figure 18 on page 84 Very few compounds display such fine detail in solution spectra The light level will be lower so the signal will have more noise the noise level depends on the wavelength and mobile phase solvents used Agilent 1290 Infinity Autosampler User Manual 83 5 Optimizing Performance 240 245 250 255 Figure 18 Benzene at 1 and 4 nm Slit Width Principle The injection volume and the sample dissolution solvent are important in controlling dispersi
91. mmunication Device name 1290 Infinity ALS TypelD G42264 Serial number PP00055050 Firmware revision 4 06 15 001 Connection settings Options Syringe 20 Seat Capillary 1 2 Max injection volume External contacts board installed use BCD port for BCD port output format Thermostat installed Rinse valve installed Define Wellplates Device name based on the module Type ID based on the module product number Some modules may allow changing the type based on hardware firmware This results in a change of features and functions Serial number based on the module Firmware revision based on the module Options lists installed options Agilent 1290 Infinity Autosampler User Manual Using the Module 4 Main Screens of the Autosampler with Agilent Instant Pilot G4208A Below the main screens for the use of the autosampler are shown The Control screen allows System On Off System Get Ready System Clear Errors Welcome 1 System On Off 2 System Get Ready 3 System Clear Errors 1290 Infinity F 4 Bin Pump Seal wash HIP ALS Wash needle 5 Bin Pump Purge 1290 Infinity 6 Bin Pump Prime 7 H P ALS Wash Needle 1290 Infinity 8 DAD Balance E 9 More a a Bin Pump 1280 Infinity G4220A LP00000005 J 14 05 Method Sequence Status Logbook Morea 2 The System Info screen lists details of the autosample
92. module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations 90 Agilent 1290 Infinity Autosampler User Manual Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status f L E t _s Figure 19 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Agilent 1290 Infinity Autosampler User Manual 91 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 92 When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when
93. module will turn into 19200 baud 8 data bit with no parity Agilent 1290 Infinity Autosampler User Manual 203 12 Hardware Information Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 18 Boot Resident Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 19 Forced Cold Start Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1
94. motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Motor belt tension too high Switch off the module at the power switch Wait at least 10 minutes before switching on again 4 Defective motor Please contact your Agilent service representative 5 Defective transport assembly flex board Please contact your Agilent service representative 112 Agilent 1290 Infinity Autosampler User Manual Invalid Vial Position Error ID 4042 Error Information 7 The vial position defined in the method or sequence does not exist The reflection sensors on the transport assembly flex board are used to automatically check which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause 1 Incorrect tray installed 2 Incorrect tray definition 3 Incorrect vial positions defined in the method or sequence Suggested actions Install the correct trays or edit the method or sequence accordingly Install the correct trays or edit the method or sequence accordingly Install the correct trays or edit the method or sequence accordingly 4 Tray recognition defective dirty sample tray Ensure t
95. n Table 10 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 196 Agilent 1290 Infinity Autosampler User Manual Hardware Information 12 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a
96. n b Find initial positions for X Z and theta motors c Check belt tension of theta motor d Determine transmission ratio for X and theta axes Turn needle carrier fully counter clockwise theta min Move X slide into left end stop X min Move X slide into right end stop X max Rotate needle carrier fully clockwise theta max happens at the same time as step iii Read RFID tag of Sampling Unit Read RFID tag of sample tray if tray is different from last time Move needle into needle seat to determine the seat depth Move needle into seat use depth value from step 5 Lower the needle lock on oOo ot FF Q Switch the injection valve to mainpass Agilent 1290 Infinity Autosampler User Manual 191 12 Hardware Information Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket
97. n Prepare run Low 80 3 3 Gray Start Low S NC 4 Blue Shut down Low 0 5 NC 5 Pink Not 1e connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1290 Infinity Autosampler User Manual 179 11 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 180 Agilent 1290 Infinity Autosampler User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to Genera
98. n and or Instant Pilot G4208A with the appropriate revisions see Performance Specifications on page 28 Preparations Locate bench space Provide power connections Unpack the module CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1 Place the Autosampler in the stack see Optimizing the Stack Configuration on page 34 2 Ensure the power switch on the front of the module is OFF switch stands out Agilent 1290 Infinity Autosampler User Manual 43 3 Installing the Autosampler Installing the Autosampler NOTE 3 Connect the power cable to the power connector at the rear of the module ea ge REMOTE AS 292 CAN CAN CAN DC OUT Figure 13 Rearview of Autosampler 4 Connect the CAN cable to other Agilent 1290 modules Connect the APG remote cable optional for non Agilent instruments 6 Turn on the power by pushing the button at the lower left hand side of the module The power button stays pressed in and the status LED should be green When the line power button stands out and the green light is off the module is turned off
99. n uncovered needle is a risk of harm to the operator gt Be careful working at the needle carrier assembly gt Use the silicon safety tube supplied with every new needle It is recommended to always exchange the needle assembly and the needle seat at the same time to prevent premature leakage Agilent 1290 Infinity Autosampler User Manual 141 9 Maintenance Installing the needle assembly 1 Push the silicon safety tube delivered with every needle over the needle 2 Insert the loop capillary into the needle assembly and tighten the fitting hand tight 3 Pinch the holder clamp and reinsert the needle assembly into the needle carrier 4 Attach a 5 16 inch wrench to hold the position at the needle assembly Use a 1 4 inch wrench to tighten the fitting of the loop capillary 142 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 5 Close the leak guide Next Steps 6 Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the Autosampler is calculated from the needle pusher position 7 Remove the silicon safety tube from the needle 8 In the user interface exit the Change needle seat function and exit the maintenance mode In the Lab Advisor software the Change needle seat function can be fo
100. nfinity Autosampler User Manual Optimizing Performance 5 How to Achieve High Throughput The injection can be optimized for speed remembering that drawing the sample too fast can reduce the reproducibility Marginal gains are to be made here as the sample volumes used tend towards the smaller end of the range in any case A significant portion of the injection time is the time taken with the needle movements to and from the vial and into the flush port These manipulations can be performed while the previous separation is running This is known as overlapped injection and it can be easily turned on from the autosampler setup screen in the control software The autosampler can be told to switch the flow through the autosampler to bypass after the injection has been made and then after for example 3 minutes into a 4 minutes run to start the process of aspirating the next sample and preparing for injection This can typically save 0 5 to 1 minute per injection Agilent 1290 Infinity Autosampler User Manual 75 5 Optimizing Performance How to Achieve Higher Resolution 76 Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section explains how resolution can be increased by examining the following points e Optimize selectivity e Smaller particle size packing e Longer Columns e Shallower gradi
101. ng higher sensitivity 80 dimensions 27 E electrical connections descriptions of 192 electrostatic discharge ESD 157 EMF early maintenance feedback 205 error messages arm movement 105 autosampler 104 compensation sensor open 101 compensation sensor short 102 fan failed 102 front door error 104 initialization failed 110 invalid vial position 113 leak sensor open 101 leak sensor short 100 leak 103 lost CAN partner 100 metering home failed 111 missing vial 109 motor temperature 112 needle lock failed 107 needle to needle seat position 108 peristaltic pump error 113 rear blind seat missing 115 remote timeout 99 shutdown 98 timeout 98 valve to bypass failed 106 valve to mainpass failed 106 vessel error 114 Agilent 1290 Infinity Autosampler User Manual vessel stuck toneedle 114 exchanging needle seat 144 external contact cable 184 extra column volume 70 F fan failed 102 firmware description 188 main system 188 resident system 188 update tool 189 updates 189 158 upgrade downgrade 158 flow cell Max Light cartridge flow cell 80 Max Light high sensitivity cell 80 frequency range 27 G general error messages 98 H humidity 27 l injection volume achieving higher volumes 73 injector steps 130 installation bench space 26 installing metering seal 152 instrument layout 206 interfaces 194 internet 219 L LAN cable 183 leak sensor open 101 leak sensor short 100 leak 103 line fre
102. nt 1290 Infinity Autosampler User Manual Error Information 7 Metering Home Failed Error ID 4054 4704 The metering piston has failed to move back to the home position The home position sensor on the sampling unit flex board monitors the home position of the piston If the piston fails to move to the home position or if the sensor fails to recognize the piston position the error message is generated Probable cause Suggested actions 1 Defective sensor or main board Please contact your Agilent service representative 2 Broken plunger Exchange the metering plunger and seal 3 Defective metering drive motor Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual 111 7 Error Information Motor Temperature Error ID 4027 4040 4261 4451 One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched off the motor to prevent damage to the motor Motor identification e Motor 0 temperature X axis motor e Motor 1 temperature Z axis motor e Motor 2 temperature Theta motor The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the
103. ntenance might be required yellow EMF warning Maintenance required red Agilent 1290 Infinity Autosampler User Manual Using the Module 4 Agilent ChemStatio Setting up the Autosampler wit Control Settings These settings are available via right click on the Active Area of the ALS GUI ical Missing Vessel The handling of missing vessels can be aaa configured Illumination Can be turned on off On Linked Pump To configure which pump delivers flow to off the Autosampler Prime Flush Pump Priming the Needle wash flush pump Linked Pump G42204 LP00000005 Agilent 1290 Infinity Autosampler User Manual 55 Method Parameter Settings Setup Method These settings are available via Menu gt Instrument gt Setup Agilent 1290 Infinity Autosampler or via right click on the Active area The signal window in the lower part is not shown when opening the parameter settings via right mouse on the Autosampler user interface amp 1290 Infinity Autosampler oy 1290 Infinity Autosampler Injector Program Injection Mode Injection volume Mode Time Location Repeat Stoptime No Limit Advanced 100 pl Ausiliary Draw speed Standard injection Eject speed Draw position 0 0 mm Equilibration time 20 gt sec Sample flush out factor 5 0 times injection volume Flush Port Vial Well bottom sensing High throughput Automatic delay volume reduction Enable overlapped injection Po
104. nts for Different Purposes Activity Solvent Comments After an installation lsopropanol Best solvent to flush air out of the system When switching between lsopropanol Best solvent to flush air out of reverse phase and normal phase both times After an installation To clean the system when using buffers After a solvent change Ethanol or methanol Bidistilled water Bidistilled water the system Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Agilent 1290 Infinity Autosampler User Manual Using the Module 4 Setting up the Autosampler with Agilent ChemStation The setup of the Autosampler is shown with the Agilent ChemStation B 04 02 Depending on the controller e g Agilent Instant Pilot EZChrom Elite the screens look different For the Instant Pilot refer to Main Screens of the Autosampler with Agilent Instant Pilot G4208A on page 61 This section describes the autosampler settings only For information on the Agilent ChemStation or other 1290 Infinity modules refer to the corresponding documentation or the 1290 Infinity System Manual After successful load of the ChemStation you should see the module as an active item in the graphical user interface GUI Agilent 1290 Infinity Autosampler User Manual 51 4 Using the Module Setting up the Autosampler with Agilent ChemSt
105. o column diameter will be less and the sensitivity will increase For example decreasing the column i d from 4 6 mm to 2 1 mm results in a theoretical gain in peak height of 4 7 times due to the decreased dilution in the column For a mass spectrometer detector the lower flow rates of narrow columns can result in higher ionization efficiencies and therefore higher sensitivity Agilent 1290 Infinity Autosampler User Manual 79 5 80 Optimizing Performance How to Achieve Higher Sensitivity for Detector The detector has a number of parameters that are used to optimize performance The following sections describe how the detector parameters affect performance characteristics e Flow cell affects sensitivity Wavelength and bandwidth affect sensitivity selectivity and linearity e Slit Width affects sensitivity spectral resolution and linearity e Peak Width affects sensitivity and resolution Flow Cell The Max Light cartridge flow cell has a standard 10 mm path length and is optimized for minimal volume and dispersion 6 volume 1 0 uL It has high light transmission minimizing noise to reduce noise due to the optofluidic waveguide It is suitable for use with a wide range of analytical columns from short narrow bore columns to long standard diameter 4 6 mm columns Generally the peak dispersion volume calculated from peak width x flow rate should be greater than about 2 uL for this cell for example 0 02 min x 200 uL min
106. od and instructions are carefully followed Agilent 1290 Infinity Autosampler User Manual 1 1 20 Introduction System Overview Figure 6 Leak and waste handling concept overview typical stack configuration as an example Agilent 1290 Infinity Autosampler User Manual Introduction 1 The solvent cabinet 1 is designed to store a maximum volume of 6 L solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet The leak pan 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 8 A guides excessive overfill from one module to the next as the solvent flows into the next module s leak funnel 8 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to waste The condense drain outlet of the autosampler cooler 5 guides con
107. oduction Control of the vial plate temperature in the thermostatted autosampler is achieved using an additional Agilent 1200 Series module the Agilent 1200 Series thermostat for ALS FC Spotter The thermostat contains Peltier controlled heat exchangers A fan draws air from the area above the sample vial tray of the autosampler It is then blown through the fins of the cooling heating module There it is cooled or heated according the temperature setting The thermostatted air enters the autosampler through a recess underneath the special designed sample tray The air is then distributed evenly through the sample tray ensuring effective temperature control regardless of how many vials are in the tray In cooling mode condensation is generated on the cooled side of the Peltier elements This condensed water is safely guided into a waste bottle for condensed water Agilent 1290 Infinity Autosampler User Manual Introduction 1 Autosampler Principle The movements of the autosampler components during the sampling sequence are monitored continuously by the autosampler processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosampler by the injection valve during the sampling sequence The needle moves to the desired sample position and is lowered into the sample liquid in
108. of plate used All axes of the transport mechanism x z theta robot are driven by stepper motors Optical encoders ensure the correct operation of the movement The standard metering device provides injection volumes from 0 1 20 ul A 0 1 40 ul injection volume metering device is installed in the G4226A with a 20 ul low restriction loop capillary restricting the injection volume The entire flowpath including the metering device is always flushed by the mobile phase after injection for minimum internal carry over An additional needle flush station with a peristaltic pump is installed to wash the outside of the needle This reduces the already low carry over for very sensitive analysis The bottle containing the mobile phase for the wash procedure will be located in the solvent bottle cabinet Produced waste during this operation is channeled safely away through a waste drain The six port only 5 ports are used injection valve unit is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through the autosampler which ensures that all of the sample is injected onto the column and that the metering unit and needle are always free from sample residue before the next sampling sequence begins Agilent 1290 Infinity Autosampler User Manual 11 1 12 Intr
109. ology sub two micron narrow bore columns with the Agilent 1290 Infinity LC System Increased robustness is achieved by optimized new parts high speed with lowest carry over by flow through design increased sample injection speed for high sample throughput increased productivity by using overlapped injection mode and flexible and convenient sample handling with different types of sample containers such as vials and well plates Using 384 well plates allows you to process up to 768 samples unattended For specifications see Performance Specifications on page 28 This 1290 Infinity Autosampler has been introduced together with the Agilent 1290 Infinity Liquid Chromatograph Agilent 1290 Infinity Autosampler User Manual Introduction 1 Overview of the Module The Autosampler transport mechanism uses an X Z theta robot to optimize the positioning of the sampling arm on the well plate Once the sampling arm is positioned over the programmed sample position the programmed sample volume is drawn by the metering device into the sampling needle The sampling arm then moves to the injection position where the sample is flushed onto the column The autosampler employ a vial plate pusher mechanism to hold down the vial or the plate while the needle is drawn back from the sample vessel a must in the case a septum is used This vial plate pusher employs a sensor to detect the presence of a plate and to ensure accurate movement regardless
110. ometer is the detector Operating settings of the autosampler allow the user to set appropriate parameters to minimize carryover in any application involving compounds liable to stick in the system The following functions of the autosampler can be used to minimize carryover Internal needle wash External needle wash e Needle seat backflush e Injection valve cleaning The flow path including the inside of the needle is continuously flushed in normal operation providing good elimination of carryover for most situations Automated delay volume reduction ADVR will reduce the delay volume but will also reduce the flushing of the autosampler and should not be used with analytes where carryover might be a problem The outside of the needle can be washed using a wash vial in a specific location or the needle can be washed using the flush port If a wash vial in a tray location specified by the user is chosen then this vial should have no septum and should contain a solvent suitable for washing the sample from the needle The septum is not used to avoid wiping contamination off the needle on the downstream only to re apply it on the upstroke The needle can be dipped into the vial multiple times This will be effective in removing a small degree of carryover but for more effective washing of the outside of the needle use the flushport Agilent 1290 Infinity Autosampler User Manual 87 5 88 Optimizing Performance The flush port is lo
111. on Care must be taken that the compounds are focused at the top of the column to avoid peak dispersion due to the injection which would cause a reduced peak height To achieve this the sample should be dissolved in a solvent composition of lower elution strength than the mobile phase It may be possible to increase the injection volume to have a greater concentration of analyte on the column and hence increased peak height Peak Width Response Time and Data Collection Rate The peak width setting response time and data rate in the detector are all linked The available settings are shown in Table 5 on page 86 It is important to set this correctly for optimum sensitivity and to preserve the resolution achieved in the separation The detector internally acquires data points faster than is needed for a chromatogram and processes them to produce the signal seen by the data system Part of the processing reduces the data to an appropriate data rate which allows the chromatographic peaks to be accurately drawn As with most analytical determinations groups of readings are effectively averaged to reduce error in the result The detector bunches raw data points and produces the output signal data at the required data collection rate by an electronic filtering process If the resulting data rate is too slow over filtering the peak heights will be reduced and the resolution between them reduced too fast and the data is noisier than it need be to acc
112. pillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual WARNING Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off gt Avoid loops gt Tubes must not sag gt Do not bend tubes gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module Agilent 1290 Infinity Autosampler User Manual 41 3 Installing the Autosampler Installation Information on Leak and Waste Handling Warning Attention Warnung Attenzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant tout d placement gt Entfernen Sie das Netzkabel bevor Sie das Gerat bewegen g Scollegare il cavo di alimentazione prima di spostare lo strumento Desconectar el aparato de la red antes de moverlo b 2J l Figure 12 Warning label illustration for correct waste tubing 42 Agilent 1290 Infinity Autosampler User Manual Installing the Autosampler 3 Installing the Autosampler Parts required Description Autosampler Power cord Other cables see below and section Cable Overview on page 174 ChemStatio
113. pler User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog a 3 Red Analog S e Agilent 1290 Infinity Autosampler User Manual 177 11 Identifying Cables Remote Cables 178 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1290 Infinity Autosampler User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL a 9 1 White Digital ground NC 2 Brow
114. quency 27 line voltage 27 lithium batteries 215 loop capillary change 127 lost CAN partner 100 M maintenance feedback 205 overview 160 160 136 positions 126 removing the needle assembly 138 replacing firmware 158 message remote timeout 99 metering device change 129 metering seal install 152 remove 149 missing parts 33 N needle carrier change 128 needle seat exchange 144 needle change 127 non operating altitude 27 Agilent 1290 Infinity Autosampler User Manual Index non operating temperature 27 0 operating Altitude 27 operating temperature 27 optimization achieving higher resolution 76 achieving higher sensitivity 79 achieving lowest carryover 87 column use 79 detector sensitivity 80 injection volumes 73 slit width 83 stack configuration 34 wavelength and bandwidth 80 overview autosampler 11 P packaging damaged 32 parts and materials 33 parts identification cables 173 peak width 84 peristaltic pump cartridge replace 154 physical specifications 27 power consideration 24 power consumption 27 power cords 25 power supply indicator 91 power switch 43 principle autosampler 13 remote cable 1 8 221 Index removing metering seal 149 repairs replacing firmware 158 replacing peristaltic pump cartridge 154 rotor seal 146 resolution Optimization 76 response time 84 rotor seal exchange 146 RS 232C cable 185 communication settings 202 S safety class 212
115. r Firmware revision Property Value a ti Installed Options Seal Wash Pump Solvent Selection Valve On time LAN TCP IP Mode USING STORED LAN TCP IP Address 134 40 28 111 LAN MAC Address 0030D306213B Main Board information Transport assembly information Board ID TYPE G64220 65000 SER MAC REV AA MFG ra HiP ALS PP00055050 Sampling unit information Main Revision A 06 15 001 j i i Resident Revison A 06 10 004 Syringe information On time 49d 20 50h Board ID TYPE 6 1367 66520 REV CA_CB SER 0086 Transport G4226 60019 A 00050 Sampling Unit G4226 60028 A 00050 Syringe TCC DE00000009 Main Revision A 06 16 001 Resident Revison A 06 10 004 On time 18d 00 20h zy Exit J fm 14 05 a SS eS ee E Agilent 1290 Infinity Autosampler User Manual 61 4 gt Configure HiP ALS lt Not Set gt Syringe 20 ul Seat 1 2 ul On Missing Vessel Abort Plate 1 Front lt No Plate gt Plate 2 Back lt No Plate gt _ i Flush Out Pump BINPUMP Serial Interface 19200 Baud 8 Bits None Parity Sample Illumination OFF Exit JA user defined identifier for the module KS J 14 02 System Controller I Bin Pump HiP ALS wy Method UNNAMED filtered Edit Injection Volume i 2 Injection Mode Standard Overlap Disabled ps Needle Wash 3 0 sec in Flush Port T i Wash Position V
116. r setting the pressure at which the test is performed The leak rate of high pressure parts are not always a linear function and therefore it is recommended to perform the test at a pressure that correspond to the normal operating pressure of the system When In case of a suspected leak To verify successful execution of maintenance tasks Parts required p n Description 1 01080 83202 Blank nut Preparations Solvents must be present in both channels Agilent 1290 Infinity Autosampler User Manual 119 8 Test Functions System Pressure Test 1 Run the System pressure test with the Agilent Lab Advisor for further information see Online Help of user interface General Limits Signals Test Name System pressure test for Aladdin Description Preliminary system pressure test for Aladdin Module G4220A LP00000003 Approx Time Not defined Status Passed Prepare pump pressure test System leak 2 1 bar Enter the test pressure Flush the system System checking leak rate of pump Insert blank nut System checking leak rate of system Evaluate results Restore system configuration NWS AN SS Figure 20 System Pressure Test Result Pump head leak test Enter the pressure at which the test will be executed Figure 21 System Pressure Test Dynamic pressure input 120 Agilent 1290 Infinity Autosampler User Manual Test Functions 8 System Pressure Test Evaluation System Pressure Test
117. radio frequency emissions from the instrument itself 206 Agilent 1290 Infinity Autosampler User Manual a Agilent 1290 Infinity Autosampler User Manual KP amp e 20 ee 13 7 e LAN Configuration e e e Setting up the module in a LAN environment 208 Connecting the module via LAN 209 This chapter provides information on connecting the autosampler to the Agilent ChemStation PC a Agilent Technologies 207 13 LAN Configuration Setting up the module in a LAN environment It is not recommended to connect an Agilent 1290 Infinity system via the G4226A Autosampler The G4212A Diode Array Detector is producing the most data in the stack followed by the G4220A Binary pump and it is therefore highly recommended to use either of these modules for the LAN connection 208 Agilent 1290 Infinity Autosampler User Manual LAN Configuration 13 Connecting the module via LAN If the module is being operated as a standalone module or if a connection via LAN is required regardless of above mentioned recommendation a G1369B C LAN card has to be used For installation and configuration see the G1369B C documentation Agilent 1290 Infinity Autosampler User Manual 209 13 LAN Configuration Connecting the module via LAN 210 Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 14 Appendix General Safety Information 212 Lithium Batteries Information 215 The Waste Electri
118. rosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCl Oo 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1290 Infinity Autosampler User Manual Using the Module 4 Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si04 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can n
119. rrent through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual 101 7 102 Error Information Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the ma
120. s required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2392 4 mm Hex key G4226 43800 Seal insert tool Parts required p n Description 1 0905 1717 Metering seal Preparations Removing the metering seal see Removing the Metering Seal on page 149 1 Install the new metering seal using the plastic side of the 2 Reassemble the metering device analytical head Make insert tool Press it firmly into position Avoid any offset sure to tighten screws firmly and have the tag on the angle as it might deform the seal right side looking from the front Insert tool 152 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Installing the Metering Seal 3 Press the piston into the seal 4 Reinstall the metering device analytical head to the sampling unit by tightening the two fixing screws alternately with a 4 mm hex key 5 Connect the two capillaries to the metering device using Next Steps a 1 4 inch wrench 6 Close the front door 7 Inthe user interface exit the Change Metering device function and exit the maintenance mode In the Lab Advisor software the Change Metering device function can be found in the Tools section Agilent 1290 Infinity Autosampler User Manual 153 9 Maintenance Replacing Peristaltic Pump Cartridge When Tubing blocked or broken Parts required p n 1 5065 4445 Peristaltic pump with Pharmed tubing The peristaltic pump cartridge
121. safety information lithium batteries 215 safety general information 212 standards 27 symbols 214 sensitivity optimization 79 shutdown 98 signal wavelength 82 site requirements power cords 25 slit width 83 special interfaces 200 special settings boot resident 204 forced cold start 204 specification physical 27 status indicator 92 steps 222 injector 130 step commands 131 system setup and installation optimizing stack configuration 34 T temperature sensor 103 test functions 90 timeout 98 troubleshooting error messages 90 97 status indicators 90 91 V vial trays 161 voltage range 27 WwW wavelength and bandwidth optimization 80 weight 27 Agilent 1290 Infinity Autosampler User Manual www agilent com In This Book This manual contains technical reference information about the Agilent 1290 Infinity autosampler G4226A introduction and specifications e installation e using and optimizing troubleshooting and diagnose e maintenance e parts identification hardware information e safety and related information Agilent Technologies 2011 2014 2015 Printed in Germany 02 2015 G4226 90001 Rev C fe Agilent Technologies
122. safety tube supplied with every new needle It is recommended to always exchange the needle assembly and the needle seat at the same time to prevent premature leakage 138 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Removing the needle assembly 1 Inthe user interface start the maintenance mode and 2 Open the front door and remove the side door select Change needle seat function In the Agilent Lab Advisor software the Change needle seat function can be found in the Tools section 3 Turn the Needle carrier 90 clockwise Agilent 1290 Infinity Autosampler User Manual 139 9 Maintenance Removing the needle assembly 5 Attach a 5 16 inch wrench to hold the position at the 6 Pinch the holder clamp pull back and remove the loop needle assembly Use a 1 4 inch wrench to loosen the capillary from the needle assembly fitting of the loop capillary 140 Agilent 1290 Infinity Autosampler User Manual Maintenance 9 Installing the needle assembly When When the limit in the needle into seat counter in the EMF is exceeded or when needle shows indications of damage blockage or leaks Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description G4226 87201 Needle assembly Preparations In order to avoid leaks close the shutoff valves in the pump or remove tubings from solvent bottles WARNING Risk of injury by uncovered needle A
123. se sensitivity to that molecule The detector is linear up to 2 AU and beyond for many applications This offers a wide linear range for concentration For high concentration analysis the concentration linear range can be extended by setting the wavelength to one with a lower absorbance such as a wavelength minimum or by taking a wider bandwidth which usually includes lower absorbance values The use of wavelength maxima and minima for quantitation dates back to Agilent 1290 Infinity Autosampler User Manual 81 5 82 Optimizing Performance conventional UV detectors which because of mechanical tolerances in moving gratings needed to avoid steeply sloping parts of the spectrum Diode array based detectors do not have this limitation but for reasons of convention maxima and minima are chosen in preference to other parts of the spectrum The reference bandwidth is normally set on a region of the UV spectrum in which the analyte has no absorbance This is shown in the spectrum for anisic acid in Figure 17 on page 83 This spectrum is typical of many small molecules containing a UV chromophore For best results the reference has been set so that it is a wide band as close to the signal wavelength as possible but on a zero absorbance region Reference bandwidths of 60 nm to 100 nm are commonly used The default reference is 360 nm with a bandwidth of 100 nm A wide bandwidth is used because this reduces the noise in the reference signal from stati
124. sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Agilent 1290 Infinity Autosampler User Manual 103 7 Error Information Module Error Messages 104 These errors are autosampler specific Exhaust Fan Failed Error ID 4456 4457 The exhaust fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain value the error message is generated and the module shuts down Probable cause 1 Fan cable disconnected 2 Defective fan 3 Defective main board Front Door Error Error ID 4350 4352 4458 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The front door and or the SLS board are damaged Probable cause 1 The sensor on the SLS board is defective 2 The door is bent or the magnet is misplaced broken Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual Error Information 7 Side Door Error Error ID 4355 4459 The side door and or the main board are damaged Probable cause Suggested actions 1 The door is bent or the magnet is Please cont
125. ss Duration 5 Needle Up Valve Mainpass Help et 1120 Tools overview Li 1200 Tools overview Figure 30 Injector Steps Running 130 Agilent 1290 Infinity Autosampler User Manual Step Commands Table 7 Step Commands Test Functions 8 Step Action Comments Valve Bypass Plunger Home Needle Up Move to Location Needle into Sample Draw Needle Up Needle into Seat Valve Mainpass Needle Up Mainpass Switches injection valve to the bypass position Moves the plunger to the home position Command also switches the valve to bypass if it is not already in that position Lifts the needle arm to the upper position Move the needle arm to the vial location on the plate Lowers the needle into the vial Metering device draws the defined injection volume Command lifts the needle and lowers the needle into the sample Command can be done more than once maximum draw volume of 20 uL for 40 uL and 120 pL hardware changes are required see multi draw cannot be exeeded Use Plunger Home to reset the metering device Lifts the needle out of the vial Lowers the needle arm into the seat Switches the injection valve to the mainpass position Moves needle arm to waste position and switches the injection valve to the mainpass position Agilent 1290 Infinity Autosampler User Manual 131 8 Test Functions Injector Steps 132 Agilent 12
126. stical theory the error i e noise in this case is reduced by the square root of the number of determinations It is important that the reference bandwidth does not extend to a part of the spectrum that has some absorbance as this would then reduce the resulting signal and sensitivity would be reduced The use of a reference wavelength can help to reduce drift or wander in the chromatogram caused by refractive index changes due to room temperature fluctuation or gradient operation The effect of a reference signal can be easily tested by setting two otherwise identical signals one with and one without a reference signal If there is no part of the spectrum with zero absorbance then it will be better to have the reference signal turned off Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 signal ici wavelength Anisic Acid reference bandwidth 10 0 nm 30 nm reference bandwidth wavelength 350 nm 280 30 320 Wavelength nm Figure 17 Spectrum of Anisic Acid Slit Width G4212A only Light transmission into the spectrograph and the optical bandwidth are controlled by the variable aperture entrance slit The default setting for the slit width is 4 nm which is appropriate for most applications as it gives good all round performance The performance characteristics affected are sensitivity spectral resolution and linearity Considering a particular wavelength entering the spectrograph its light wi
127. sttime Off Injection Cleaning Figure 15 Method Parameter Settings Manual Using the Module 4 Setting up the Autosampler with Agilent ChemStation Injection Mode The settable Injection volume range is from 0 1 20 0 pL Select to use Standard injection or Injection with Needle wash Needle wash It is possible to select between using the built in flush port of the Autosampler or using a non capped vial Using Needle wash is required to obtain minimum carry over An autosampler Stop Time can be set Agilent 1290 Infinity Autosampler User Manual 57 4 Using the Module Setting up the Autosampler with Agilent ChemStation Injection Cleaning The Injection Valve Cleaning section allows you to specify the valve switching times at the end of overlap or sample flush Times 1 4 are the times when the valve switches to bypass for time 1 or to mainpass and bypass for times 2 3 and 4 The times must be specified in ascending order You can also switch the times to off Between the first and second and second and third valve switches a rinse is executed using the rinse volumes specified in the Injector Cleaning section Valve movements specifies the number of times that the valve switches from mainpass to bypass at times 2 3 and 4 in the field The maximum value is 2 default is 1 58 Agilent 1290 Infinity Autosampler User Manual Injection Program Setup Method gt 1290 Infinity A
128. t to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al Og are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 5 Optimizing Performance Delay Volume and Extra Column Volume 70 Delay Volume 70 How to Configure the Optimum Delay Volume 71 How to Achieve Higher Injection Volumes 73 How to Achieve High Throughput 75 How to Achieve Higher Resolution 76 How to Achieve Higher Sensitivity 79 How to Achieve Lowest Carry Over 87 This chapter gives hints on how to optimize the performance or use additional devices ott Agilent Technologies 69 5 Optimizing Performance Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that
129. that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1290 Infinity Autosampler User Manual In This Guide In This Guide This manual covers the Agilent 1290 Infinity Autosampler G4226A 1 Introduction This chapter gives an introduction to the autosampler 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Autosampler This chapter provides information on unpacking checking on completeness stack considerations and installation of the autosampler 4 Using the Module This chapter provides information on how to set up the autosampler for an analysis and explains the basic settings 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1290 Infinity Autosampler User Manual 3 In This Guide 8 Test Functions This chapter describes the tests for the module 9
130. the sample to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close the sample loop Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The standard sampling sequence occurs in the following order The injection valve switches to the bypass position The plunger of the metering device moves to the initialization position The needle lock moves up The needle moves to the desired sample vial or well plate position The needle lowers into the sample vial or well plate The metering device draws the preset sample volume The needle lifts out of the sample vial or well plate The needle is then moved onto the seat to close the sample loop oo N ow F OMN The needle lock moves down 10 The injection cycle is completed when the injection valve switches to the mainpass position If needle wash is required it will be done between step 7 and 8 Agilent 1290 Infinity Autosampler User Manual 13 1 Introduction Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position In this position the mobile phase flows through the autosampler metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry
131. ting up the Autosampler with Agilent ChemStation 51 Control Settings 55 Method Parameter Settings 56 Module Configuration 60 Main Screens of the Autosampler with Agilent Instant Pilot G4208A 61 Solvent Information 64 This chapter provides information on how to set up the autosampler for an analysis and explains the basic settings ote Agilent Technologies 47 4 Using the Module Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Intern
132. to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components Switch OFF the autosampler at the main power switch Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the autosampler Install the interface board Secure the screws ao A Q N Reconnect the cables to the board connectors Screws Agilent 1290 Infinity Autosampler User Manual 157 9 Maintenance Replacing the Module Firmware When Tools required OR OR Parts required Preparations 158 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required mod
133. tput are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Agilent 1290 Infinity Autosampler User Manual 199 12 Hardware Information Table 12 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 200 Agilent 1
134. ts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 214 Agilent 1290 Infinity Autosampler User Manual Appendix 14 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig h ndtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1290 Infinity Autosampler User Manual 215 14 Appendix The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 216 Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC a
135. tuck to Needle Error ID 4453 The vessel sticks to the needle when Probable cause 1 Closing mat to rigid thick 2 Bad X or Theta positioning and the needle sticks into the wall between two holes 3 Defective encoder on the needle carrier assembly Suggested actions Check the vessel definition in the plate configuration Check that the closing mat is not too thick Please contact your Agilent service representative Please contact your Agilent service representative the needle moves up Suggested actions Check that the closing mat is not too thick Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity Autosampler User Manual Error Information 7 Rear Blind Seat Missing Error ID 4724 Rear blind seat is missing although claimed to exist by main board information occurs during initialization or if the blind seat location has to be used Probable cause Suggested actions 1 Blind seat is missing Install blind seat Agilent 1290 Infinity Autosampler User Manual 115 7 Error Information Module Error Messages 116 Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 8 Test Functions Introduction 118 System Pressure Test 119 System Pressure Test Evaluation 121 Sampler Leak Test 122 Sampler Leak Test Evaluation 123 Sample Transport Self Alignment 124 Maintenance Positions 126
136. ule firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent 1290 Infinity Autosampler User Manual This chapter provides information on parts material required for the module Agilent 1290 Infinity Autosampler User Manual 10 Parts for Maintenance Overview of Maintenance Parts 160 Vial Trays 161 Recommended Plates and Closing Mats 162 Recommended Vial Plates 163 Accessory Kit 163 Analytical Head Assembly 164 Injection Valve Assembly 166 Cover Parts 167 Leak System Parts 168 Upgrade Kits 170 Large Volume Injection Kit multi draw 171 ae Agilent Technologies 159 10 Parts for Maintenance Overview of Maintenance Parts 160 p n 0905 1717 5068 0007 G4226 87201 G4226 87012 G4226 60310 G4226 60013 5067 4703 Description Metering seal Injection valve rotor seal Needle assembly Needle seat Loop cartridge 20 uL 40 uL analytical head 40 uL Flex loop kit Agilent 1290 Infinity Autosampler User Manual Vial Trays Item p n 1 G2258 60011 0515 0866 G1313 09101 0570 1574 G4226 60000 o oa A Ww N G1329 43200 G1367 47200 7 G4226 60021 Agilent 1290 Infinity Autosampler User Manual Parts for Maintenan
137. umps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Agilent 1290 Infinity Autosampler User Manual 65 4 66 Using the Module Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens e High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less cor
138. und in the Tools section 9 Re install the side door and close the front door Agilent 1290 Infinity Autosampler User Manual 143 9 Maintenance Exchanging the Needle Seat When When seat is visibly damaged blocked or leaks Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch Flat head screwdriver Parts required p n Description 1 G4226 87012 Needle seat Preparations In order to avoid leaks close the shutoff valves in the pump or remove tubings from solvent bottles WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful working at the needle carrier assembly gt Use the silicon safety tube supplied with every new needle 1 Inthe user interface start the maintenance mode and 2 Open the front door select Change needle seat function In the Agilent Lab Advisor software the Change needle seat function can be found in the Tools section 144 Agilent 1290 Infinity Autosampler User Manual Maintenanc 9 at Exchanging the Needle 3 Disconnect the seat capillary from the Injection valve 4 With a flat head screw driver carefully lift out the needle seat from the holder Next Steps 5 Insert the new needle seat Press it firmly in position 6 Inthe user interface exit the Change needle seat function and exit the maintenance mode In the Lab Advisor software the Change needle seat function
139. urately profile narrow peaks 84 Agilent 1290 Infinity Autosampler User Manual Optimizing Performance 5 The peak width setting in the detector allows the user to correctly set these parameters without needing any knowledge other than sight of the chromatogram integration results to see how wide the peaks are The peak width setting should be set for the narrowest peak width observed in the chromatogram If it is set too wide it will make the peaks appear lower in height and wider and potentially less resolved and if it is set too narrow it will increase the baseline noise unnecessarily Essentially the software uses this value to set the data collection rate such that it collects enough data points over the narrowest peaks and it is aiming for 15 to 25 points across a peak The 1290 Infinity DAD can collect at a maximum 160 Hz if required which would allow enough data points to be collected over a peak that is only 0 1 s wide The response time setting is another way of indicating how this filtering is set It is measured in seconds and is about one third of the peak width value which is measured in minutes It effectively shows how quickly the plotted signal responds to a step change in the input signal The full spectra is not available under all conditions Based on the data points the scan data rate is reduced see Table 5 on page 86 Agilent 1290 Infinity Autosampler User Manual 85 5 Optimizing Performance Table 5 Peak Width
140. utosampler 1290 Infinity Autosampler Injector Program Append Imset Delete Gearan Moveun or ee ro Agilent 1290 Infinity Autosampler User Manual Using the Module Setting up the Autosampler with Agilent ChemStation The pretreatment injector program comprises a series of numbered lines each specifying an operation that the autosampler carries out sequentially When you activate a pretreatment injector program it replaces the standard injection cycle Select Append to add the contents of the edit line to the end of the table Select Insert to insert the contents of the edit line above the currently selected line Select Delete to delete the currently selected line Select Clear All to clear all pretreatment injector program functions from the table Select Move up to move the currently selected line one position up in the order of execution Select Move down to move the currently selected line one position down in the order of execution Select Cut to delete the currently selected line and place it on the clipboard Select Copy to copy the currently selected line to the clipboard Select Paste to paste the line on the clipboard at the current position 4 59 4 Using the Module p ine A tosam Module Configuration These settings are available via menu Instrument gt More Agilent 4220A gt Autosampler Configuration 1100 1200 HipALS Configuration Instrument 1 Co
141. valve at the high percentage of organic phase after the last peak has eluted It is also recommended to switch the injection valve again after the initial conditions for the mobile phase have stabilized This ensures that the bypass groove in the rotor seal of the valve contains the gradient start conditions which is especially important for flow rates below 0 5 ml min For samples where the outside of the needle cannot be cleaned sufficiently with water or alcohol from the flush pump use wash vials with an appropriate solvent With an injector program several wash vials can be used for cleaning Agilent 1290 Infinity Autosampler User Manual Agilent 1290 Infinity Autosampler User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 90 Status Indicators 91 Power Supply Indicator 91 Module Status Indicator 92 User Interfaces 93 Agilent Lab Advisor Software 94 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces Apg Agilent Technologies 89 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the
142. y block the capillaries and valves Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric and nitric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive to stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt 2COCI 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removed the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel Agilent 1290 Infinity Autosampler User Manual 49 4 50 Using the Module e Priming and Purging the System When the solvents have been exchanged or the system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel Therefore priming and purging of the system is required before starting an application Table 4 Choice of Priming Solve

Agilent 1290 Infinity Autosampler User Manual

Contents

Download Pdf Manuals

Related Search

Related Contents