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Service Manual - Frank`s Hospital Workshop

1. 63 9 2 3 Format of Acknowledge of the Receiver 63 9 2 4 Detailed asa etc ea toca ER E n uS M UE 63 9 2 5 lt T REEL 63 9 2 6 DATA Package een 64 9 2 Transmission Example rae cans nee E PA 66 9 3 Recommended 67 9 4 HUMACOUNT tubing 5 ea II 68 9 5 MUMACOUNT TUBING SCliermialig cusam ra 69 9 6 MUMAGOUNT PEUS Cabling N 70 9 7 FIUNIACGCOUNHT DIA gt a sroine 71 9 8 HUMACOUNT PLUS Amplifier High voltage 4 HGB Boards 72 9 9 HUMAC OUNT AMPIO 73 IEUMACOUNTPEUSAMPINERPEB nennen 74 941 SHOMACOUNT AMBINEFPEB zarte 75 912 H amp V DIE VMM EDOplo and Valve Boards anna 76 29 Hoi O 77 Sad REVISION EHI SIOLV DM MR sasa 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS 3 77 This side left blank 4 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 1 INTRODUCTION Since HUMACOUNT PLUS and HUMACOUNT have so much common characteristics we cover both instruments in a common Service Manual Information herein applies for both analyzers unless otherwise noted To be well up in the instruments please read this manual carefully to have the knowledge for ser
2. E T MOST C ue pe zs nor rs 285 H at i Ar FL ae Cas er Po ELIT 1 werd 21 TEP Som Pe X lt gt TUE 1 T A xor T I2ANI Lu m E T n i TORE say 239 5 5 042 t 7 1 m a AOSZ NOZZ 172100 qo OTH ZTAND ZANIH 4 Zu a Service Manual HUMACOUNT amp HUMACOUNT PLUS 72 77 N Human 9 9 HUMACOUNT Amplifier lt ok lt Service Manual HUMACOUNT amp HUMACOUNT PLUS 73 77 9 10 HUMACOUNT PLUS Amplifier PCB WBC 25 OOO N en Q N T4 gt lt TG IC10 Service Manual HUMACOUNT amp HUMACOUNT PLUS 74 77 N Human 9 11 HUMACOUNT Amplifier PCB Service Manual HUMACOUNT amp HUMACOUNT PLUS 75 77 9 12 H amp V DIL VM LD Opto and Valve Boards 2 E M 3 EM SONA ONG 0140 QU HA TIO A SH Shoe 017 TA bumassssananas een E E E E E eee ene eee INA i
3. e kah k b al T ER em D1 1 as cc Eco LES a WM a Connection to P cable h 2 Diotron LTD PcPROVI 6 Diatron LTD PCPRO 1 6 The pressure sensor tolerates maximum 5 V and it has the same power voltage as the opto sensors have Do not connect higher voltage than 5 V to this board because it can damage the pressure sensor Service Manual HUMACOUNT amp HUMACOUNT PLUS 13 77 2 1 12 Stepper Motor Opto Boards There are three stepper motors in the system Horizontal and Vertical motors which make the movements of the aspirating tip moving carriage and Dilutor motor which moves the three syringes macro lyse micro The stepper motor opto boards make the connections between the motor controller PlCs and motors and have opto switches for the motor s end and home positions The actual status of the stepper motor s optos is indicated by two LEDs on both stepper motor opto boards H amp V motor opto board connects to the horizontal and vertical stepper motors and to the MPNIF card through HM and VM ribbon cables On the other side of this board there are four opto switches of the two stepper motors positions home and end for both and its own indicator LEDs H amp V motor opto board Dilutor motor opto board Dilutor motor opto board contains the opto switches of the dilutor motor which moves the syringes in the fluidic s
4. 4 DrainWbc X X V1 DrainMix x Mi a tui 1 i CQ waste Fur se Waste Full Sensor Micro Pressure Puffer Reservoir In HUMACOUNT the RBC counting process is absolutely the same as the WBC from pneumatical point but the diluted sample which is aspirated by the regulated vacuum from the chamber is the second dilution rate made by the second dilution process RBC counting time is also 8 seconds 3 8 Washing processes Both instruments make the necessary washing processes such as MIX WBC and RBC chambers washing processes in HUMACOUNT PLUS and chamber washing process in the HUMACOUNT The systems has a two step washing method which ensures a very low carry over between measurements At the first step called pre washing 1 ml of diluent washes the actual chamber and the pump drains it At the next step called main washing the actual chamber is primed with diluent This diluent volume is higher 0 2 ml than the chamber s operating sample volume Sometimes for example the MIX washing in HUMACOUNT PLUS this second diluent is not drained immediately after the washing process it stays in the chamber protecting it from contamination In this case the chamber is drained at the end of the total washing process Draining is a very simple fluidic method The Pump aspirates the fluid from the actual chamber or tube through its draining valve is On while the other valves are
5. Bottom opto OM2 sensor but OM3 sensor is not lt 2 Gate opto 2 1 4 Amplifier Boards HUMACOUNT PLUS and HUMACOUNT amplifier boards are different PCBs but there are a lot of common functional parts on them Common functional parts amplifier board includes its own voltage regulator the connection interfaces for HGB head for high voltage board and for MPNIF card In this board there is the current generator circuit which works from 50 V measuring voltage coming from high voltage board and the probe DC voltage is amplified with a voltage follower output ELV Nominal measuring current is 870 pA The amplifier board includes the main 3 stage amplifier channel which amplifies the input signal to the 0 5 V range this is the input range of the A D converter which is on the MPNIF card The offset voltage is set by P1 potentiometer by the manufacturer Adjust the offset voltage only in case it is out of the 5mV range The test circuit makes possible to generate test pulses with TEST and PLS signals through FETs for checking the proper operation of the amplifier channel 8 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human HUMACOUNT PLUS amplifier board includes also the connection JP2 for volume opto limiter In this board there are two reed relays on the input side IC10 can select between the two channels RBC WBC with RSW signal IC11 connects high voltage from high voltage board to
6. Romedy HGB dark HIGH Instrument door open Close instrument door ada Check HGB head connections or LOW HGB LED out of order check HGB LED operation during measurement HGB light HIGH Instrument door open or Close door or HGB LED too bright replace HGB head LOW Fault on High Voltage Check measuring voltage 50V on Electrode voltage pi Hiah volt or HIGH or Amplifier board ign voltage and LOW Check current generator relays Current Fault on Amplifier board and test generator FET on or HIGH Amplifier board Offset LOW Unadjusted Offset on Adjust the offset potentiometer on or HIGH Amplifier board Amplifier board LOW MPNIF and Amplifier Boards Check cables and connectors Amplifier test are not connected between MPNIF and Amplifier LO O HIGH Instrument not grounded Check mains ground lead W Check current generator relays Peak of pulses Fault on Amplifier board and test generator FET on or HIGH Amplifier board HIGH Instrument not grounded Check mains ground lead HIGH Instrument not grounded Check mains ground lead LOW Etpa gabie Check pressure sensor on PRO Outer pressure amolifier or PIC 0 B board cable connections and or HIGH MPNIF board pressure amplifier LOW Peristaltic pump failure Check peristaltic pump amp tube HIGH Leakage in pneumatics Check tubing in pneumatics Service Manual HUMACOUNT amp HUMACOUNT PLUS 39 77 5 2 Service
7. 40 5 2 1 lt AA 40 92 2 Mellitus e nenn 40 923 oo 40 Daza DEVICE INLONMALON gt aun om e 40 0 205 40 920 41 927 Testing Mehl 42 952 0 Valve Test MENU mmm 42 5 2 9 Display and Keyboard TES 43 9 2 10 MONG ee 43 Sil Museum E E 43 5 2 12 Rescue Code for Multi user 43 SERVICE OPERA TIONS 44 6 1 a 44 6 2 esse 45 6 2 1 FREMOVING DIMM Gasen 45 6 2 2 MANN OIMNE AA ap 45 6 3 MDA Monochrome Display Adapter emulation 45 6 4 KEY BIOS settings TOF Corect operation u uuu uu au 46 6 4 1 BIOS Sellhgs or DIMP ute m bum uqa 46 OZ EXIT MENU uu aaah 47 6 5 DOS Funetions u u u u uu uy u enge 47 6 6 Fi O Messages 48 6 6 1 EO COGE ee 48 6 6 2 Message COCS d AA AA 51 2 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS 6 7 Problems related to Warning 05
8. A718 Valve I 6 10 cable HUMACOUNTPLUS 58 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS CODE A719 A720 A721 A722 A723 A724 A725 A726 727 C130 131 132 140 196 400 406 500 501 C503 C510 C600 C611 C612 C620 C621 C624 C630 C712 C713 C714 C715 C716 C717 C719 C720 Human NAME HUMACOUNT HUMACOUNT PLUS We I 11 15 cab HUMACOUNTPLUS Pressure boar cae HUMACOUNT PLUS oo prpiier board cable HUMACOUNTPLUS Angie boar HUMACOUNTPLUS o HVB board cable HUMACOUNTPLUS DD e BCE LPTEUSB bon cab EC Semping bar HUMACOUNT Service anual HUMACOUNT 8 HUMACOUNT PLUS 59 77 CODE NAME HUMACOUNT HUMACOUNT PLUS C721 Amplifier board cablel HUMACOUNT J t ___ C722 Amplifier board cable I HUMACOUNT J o gt o C723 HVB board cable HUMACOUNT O 0 t 5200 5203 5205 5209 5210 5211 5214 5221 5222 60 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 9 APPENDICES 9 1 Warning Flags Flag Meaning Recommended user action WBC three part 2 Repeat the measurement Possibly lyse problem warning or WBC three Check the discriminators in the WBC histogram If the discriminators are part diff Unsuccessful in the proper place the populations can be separated by eye then the results are correct No WBC three p
9. M yj ny pa 2 71 68064000020 j DEL ZEZE ZLOBONd 32 Mbytes RAM SanDisk controller m LE z gt LE d 2 Le 177 s Pung t mg ma IIT AMD Elan SC520 Super Edgeconnector 2 1 16 2 1 17 Switching Power Supply The analyzer works with a standard 200 or 230VA ATX type PC switching power supply The power supply has its own internal fuse and a built in fan The most frequent problem is the malfunction of the fan so check and clean it regularly The power supply has a temperature sensor which can detect overheating and shut down the power 2 1 18 Floppy Disk Drive The built in Floppy Disk Drive makes possible to save data on floppy disks and to install or upgrade the software normal case and for SW upgrade the boot device priority should be Hard Drive then Floppy drive in BIOS Setup In this case the analyzer starts up from SanDisk Boot Device Priority submenu for Normal operation Hard Drive Removable Devices If you need to boot from Floppy disk set up the following boot order In this case it is possible to boot from an external floppy disk containing bootable code e g MS DOS
10. V9 Cle aner 2 4 III V10 DilWbc N3 V7 FloatUp V3 FloatDown m 2 1 ha Ver V8 AspWbc Pressure Meter Puffer Reservoir V1 DrainMix V6 DrainWash X n HUMACOUNT there is also another sampling process for the second RBC dilution 25 ul of primary dilution is aspirated by the aspirating tip from the chamber but it is kept in the aspirating tip during the WBC measurement and the cleaning process Sample V11 DilWash gt i par V13 DilNeedle 2 1 4 V12 DilChamber DILUENT V14 LyseWbc 2 X 0 3 Lyse Diluent amp Cleaner V5 Cleaner Sensor Sensor AH CLEANER Micro Lyse Dil 3 NS x Pressure Meter RBC WBC LYSE Puffer Reservoir V1 DrainChamber x X V2 DrainAperture WASTE 28 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 3 3 Aspirating tip washing process Both instruments clean the aspirating tip with diluent in the washing head after sampling It is important to clean the outer surface of the aspirating tip to avoid inaccurate sampling The Macro syringe doses and the pump drains the diluent from the washing head while the aspirating tip moves upwards so the total length of it is washed and cleaned This process is called total aspirating tip washing There is another process which is washing only a part of the aspirating tip is the same but the
11. 1 4 i x gt E T T TUTUYEYUCYUYCYTC 1 E P 2 gt i i 1 mi x 5 Duo E ut maa uu s AAA A 1 AMU H Sn iq 1 LT Try GH 1 3 AS33390 0 2 RAT Quen C ry ae L H o 1 F t T o ERPPEPPFFPPPPESPPPEFTTLPERTFPERTTLERRTCPRe i i Ps 70 77 Service Manual amp HUMACOUNT PLUS 9 7 i Y J 2 sl 1 ih 9 2 m m BA m Pe De LA BO cH OH PALA PO im Ln e H MM 4 4 4 OO NOTI 1 AASA masa 3 gu ni 1 VALVE BOARD ECRIRE D IDEEPROM BHELIMATIC AMIEBEBCGE ROAR uu tae Bene ee PPL P PO Lf kka Fy U Pe fi l mi e mE zn Tee OV seo 4 Amin LCD maa ME ASUREMENLT n Li meam 7 t TL ase Se ot m J lt 7 pun LL l 1 jasuszerunA Ba w i pa a e x o
12. 52 6 8 Causes ee es ne ne 53 6 8 1 Contaminated ee 53 6 8 2 Bad Earth 9roUndINg ee 53 6 8 3 External electrical ee a ta 53 6 8 4 Ihternalunelse SOUrGES zuteil 54 MAINTENANGE cmm 55 7 1 Weekly Maintenance DY USER uu uuu u uuu 55 7 1 1 Cleaning The washing 55 7 2 Maintenance SEN AA a simu 55 7 2 1 Check Self test and Device statistics 55 7 22 Cleaning and Greasing nah u au 55 723 Checking and Lubricating Dilutor Piston tu EON 55 7 2 4 Cleaning and Lubricating Needle Moving Mechanics 55 7 29 Checking and Replacing Washing Head a 55 7 2 6 Checking and Replacing Peristaltic Pump 55 7 2 7 Checking of the Power __6___________ 55 129 Bleaching of SV aaa ea ac eg 55 SPARE a db ETE EE I I T T 56 9 APPENDICE ade sede ee n 61 9 1 Waking cia EU 61 9 2 Serial Protocol V2 X ale 62 9 2 1 BC PLA 62 9 2 2 Format of Packages Sent HUMACOUNT PLUS HUMACOUNIT
13. 2 Place the DIMM PC with edge connector to the socket with Heatsink up at approx 30 angle and push it softly into the socket Do not to force it because the socket can be damaged Pull apart the white holder on both sizes and snap the module into place by pushing it down 3 Make sure the levers are back in place keeping the module down 4 Screw in the safe lock screw with the plastic ring M2 x 10 mm hexagonal DimmPC is the trade mark of Kontron Embedded Modules GmbH 63 MDA Monochrome Display Adapter emulation mode MDA emulation mode was developed to provide video output to be displayed on the LCD This mode is available during the total operation but especially useful for checking the BIOS setup and OS functions This mode should be handled the same as the service menu the user does not have to know these options At power on the controller PIC on MPNIF card is configuring the FPGA chip and displays the greeting screen After 6 seconds it starts to scan the keypad and behaves like an MDA adapter to the PC although the displayed screen is still holding the greeting screen If you switch to MDA emulation mode the system boot events can be displayed and the CMOS SETUP program can be started if necessary If the main program does not start in 90 seconds the PIC will automatically switch to MDA displaying mode to give information about the reason of hang up This can be caused by some CMOS failure e g there is a non boota
14. You can refer to the floppy disk as A SanDisk will be C Boot Device Priority submenu for booting from a bootable floppy disk Removable Devices Hard Drive 2 1 19 Parallel and USB Printer Ports The Printer port located on the rear panel is for external printers with parallel port The USB port is an option If DimmPC is equipped with USB module and at least SW V3 0 is installed the analyzer supports USB printer connection 2 1 20 Serial Port The Serial port is a standard RS232 V24 port with the standard pin out COM1 marked Serial can be used is useful for communication with a host computer 16 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS 2 2 Main mechanic and fluidic parts of the Analyzer Human HUMACOUNT PLUS HUMACOUNT Hematology Analyzers consist of the following mechanic and fluidic parts 1 2 3 4 5 6 7 8 9 1 Dilutor H amp V moving unit Volume meter tube xVM only for HUMACOUNT PLUS Pump Aspirating tip Chamber s Valves 1 5 6 10 11 15 Washing head Puffer reservoir 0 Tubing V11 DilWash V13 DilNeedle 2 1 lt EITE alls v9 X V14 LyseWbc Cleaner INE ZN AX V10 DilWbc V3 FloatDown 2 1 Pressure Puffer Reservoir x L V4 DrainWbc ar s gt V2 DrainRbc AXE 2 way Valve Ng Closed Off N Open On V1 DrainMix V6 Dra
15. 215 lt gt 233 lt gt 246 lt gt 254 lt gt 255 lt gt 249 lt gt 240 lt gt 227 lt gt 213 lt gt 200 lt gt 187 lt gt 176 lt gt 164 lt gt 153 lt gt 141 lt gt 129 lt gt 118 lt gt 108 lt gt 99 lt gt 92 lt gt 86 lt gt 81 lt gt 76 lt gt 71 lt gt 67 lt gt 64 lt gt 62 lt gt 61 lt gt 60 lt gt 60 lt gt 60 lt gt 57 lt gt 55 lt gt 51 lt gt 47 lt gt 44 lt gt 43 lt gt 42 lt gt 42 lt gt 43 lt gt 44 lt gt 45 lt gt 47 lt gt 47 lt gt 46 lt gt 45 lt gt 43 lt gt 40 lt gt 37 lt gt 34 lt gt 32 lt gt 30 lt gt 3 0 lt gt 30 lt gt 30 lt gt 29 lt gt 28 lt gt 26 lt gt 24 lt gt 21 lt gt 19 lt gt 17 lt gt 15 lt gt 14 lt gt 13 lt gt 12 lt gt 13 lt gt 13 lt gt 13 lt gt 1 3 lt gt 13 lt gt 12 lt gt 11 lt gt 11 lt gt 11 lt gt 12 lt gt 12 lt gt 14 lt gt 15 lt gt 16 lt gt 17 lt gt 17 lt gt 17 lt gt 17 lt gt 17 lt gt 17 lt gt 17 lt gt 17 lt gt 17 lt gt 18 lt gt 19 lt gt 21 lt gt 22 lt gt 2 3 lt HT gt 24 lt HT gt 25 lt HT gt 25 lt HT gt 25 lt HT gt 26 lt HT gt 29 lt HT gt 31 lt HT gt 34 lt HT gt 35 lt HT gt 36 lt HT gt 36 lt HT gt 35 lt HT gt 35 lt
16. The moving mechanic parts have bigger arrow 2 indicating the direction of movement Only the open On valves are mentioned in this section while all the other valves are closed Off 3 1 Waste Full Checking There is a special pneumatic process in HUMACOUNT PLUS it is called Waste Full Checking Valve15 switches the measuring vacuum to the waste full sensor tube for a short period of time 10 msec The pressure meter measures the decrease of the vacuum during this time From this value the program can decide that the vacuum has aspirated fluid or air so the waste container is full or not In HUMACOUNT there is no pneumatic Waste Full Checking It is counting the volume of the reagents used and gives a message when this volume reaches the preset tank capacity You can modify the volume of waste tank in the Service menu Generally it is recommended to set the volume of waste tank 2 liters less than the real capacity of the tank Service Manual HUMACOUNT amp HUMACOUNT PLUS 27 77 3 2 Sampling process 25 yl 50 ul in pre diluted mode of blood sample is aspirated by the aspirating tip The Micro syringe makes the aspirating while the M3 Dilutor motor moves down In HUMACOUNT PLUS there is another sampling process when 25 yl of primary dilution is aspirated by the aspirating tip from the MIX chamber to the chamber for the second dilution Sample V11 DilWash lt gt TALK V12 DilFloat DILUENT
17. er Pneumatic System Board Display Module I nn u Fee Pressure 4 Needle Moving VIDEO Liquid Sensor Mechanism amp Power RAM Board Dilutor Block Motor Controllers amp Drivers Stepper Motor Opto Boards A D Converter Volume Opto Board Amplifier Board Digital O amp FPGA Valve Cellcounter ISA bus Interface Valve Driver Probe s amp HGB Interface amp Drivers Boards 5 4 N Driving Power Processor Board COMB Parallel Printer Port Parallel Port DimmPC Supply Interface RAM FlashDisk Serial Port Tus Floppy Disk Floppy Disk Serial Data Port Interface Interface Drive N USB Keyboard PS 2 Keyboard optional USB Interface interface interface Printer Port HUMACOUNT PLUS HUMACOUNT Electronic Functional Block Diagram 6 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 1 1 Cell counter Probes Impedance method is used for determination of volume and number of cells In this method a known volume of dilution is drawn through a small aperture Constant current is passed through the aperture from one side to the other Wh
18. opto wheel and the special mounting This moving unit moves the aspirating tip holding carriage and in the assembled analyzer it includes the aspirating tip and the washing head Aspirating tip holder Home opto switch H amp V motor opto board Washing head housing Horizontal motor Vertical motor The Vertical motor works with a special opto wheel for detecting home amp end positions See the adjustment section of this manual to place this wheel to the proper position Greasing of the horizontal vertical guiding rods should be done regularly using Photorub a PTFE based thin lubricant It is recommended to check and repeat greasing of guiding rods every year or after 10000 measurements Service Manual HUMACOUNT amp HUMACOUNT PLUS 19 77 2 2 4 Volume Meter Tube xVM HUMACOUNT PLUS only The volume meter tube xVM is mounted on the volume opto board see photo The tube contains a float which is moved up by diluent flow before counting Volume Meter tube During counting first it comes down the measurement is started when the top LED goes off and the movement of the Float float is proportional to the volume of WBC dilution measured Counting stops when bottom LED goes on RBC counting is performed when the float goes upwards none of the LEDs are on The arrow on the tube must point upwards when replacing tube Do not mix up the float direction during cleaning of the tube because the chamfering is not sym
19. 3 lt gt 22 lt gt 22 lt gt 21 lt gt 21 lt gt 19 lt gt 17 lt gt 13 lt gt lt gt 5 lt gt lt gt Receiver acknowledges WBC transmission and closes the link It is possible to request for PLT histogram if the acknowledge contains P instead of SPACE but it is not shown in this example lt ACK gt lt SPACE gt D 9 3 Recommended kit of tools e PC standard keyboard Screwdrivers Cross Slot Screwdrivers Philips Slot Screwdrivers O Hexagon Screwdrivers 3 5 2 5 2 0 1 5 mm sizes Pocket digital multimeter e Diagonal Cutter plier e Nipper Service Manual HUMACOUNT amp HUMACOUNT PLUS 67 77 22189 SfYld 8 LNNODVINNH 9995 Abacus Tubing Schematics VERSION 2 0 2000 04 18 Abtub10 vsd xx x 4 2 silicon tube B 6 3 silicon tube xx x 4 2 special tube v6 Burqni LNNOOVINNH 9 5 HUMACOUNT tubing schematic Arcus Tubing Schematics version 1 2 2000 12 08 ARCcs6v12 vsd 4 2 silicon tube xx x B 6 3 silicon tube 4 2 28 mam TIL small T fitting 1 6 big T fitting 2 3 adapter N Human Service Manual HUMACOUNT amp HUMACOUNT PLUS 69 77 9 6 HUMACOUNT PLUS Cabling Diagram MPNIF V1 8 S EHIELD AMP E L
20. HT gt 4 29 lt HT gt 0 lt LF gt P22 lt HT gt 8 2 lt HT gt 0 lt LF gt internal identifier date of measurement YYYYMMDD time of measurement HHMMSS sample identifier alphanumeric patient identifier alphanumeric patient name alphanumeric birthdate of patient YYYYMMDD patient sex 0 male 1 female doctor s name operator identifier alphanumeric patient type warning bits 32 bit hexadecimal PLT lower marker PLT upper marker RBC lower marker WBC upper marker WBC LYM MID marker WBC MID GRA marker number of parameters to send first parameter ID value and second parameter ID value and flag last parameter ID value and flag Remarks Position and meaning of the warning flags Bit Mask Letter Meaning 0x00001 RBC PLT clogging 1 0x00002 S RBC PLT time error 2 0x00004 RBC PLT data package errors 3 0x00008 m RBC PLT coincidence is too high Linearity error 4 0x00010 RBC peek warning 5 0x00020 RBC PLT limit warning 0x00040 RBC blank is high or no RBC blank 7 0x00080 p PLT blank is high or no PLT blank 0x00100 WBC clogging 9 0x00200 S WBC time error 10 0 00400 D WBC data package errors 11 0x00800 M WBC coincidence is too high Linearity error 12 0x01000 45 1 Too many RBC from WBC 13 0 02000 L WBC RBC limit warning 0x04000 3B WBC blank is high or no WBC blank 64 77 Service Manual HUMA
21. HUMACOUNT amp HUMACOUNT PLUS Human 4 2 2 Amplifier offset setting Amplifier offset should be between 5mV Run self test to determine whether offset is within this range If it is out of range it should be re set by the following way n Maintenance Service Password Miscellaneous menu press Offset adjustment menu Adjust the potentiometer to reach 0 mV Offset adjustment for HUMACOUNT PLUS is not so easy the potentiometer can be reached from the internal space only HUMACOUNT offers easier access for the amplifier offset setting element It is located on the measuring block Service Manual HUMACOUNT amp HUMACOUNT PLUS 37 77 5 CHECKING OF PROPER OPERATION Both analyzers have built in Self test and Service menu 5 1 Self test 5 1 1 Self Test Screens oelf test can be used to check the operation of the instrument Self test results Date of testing 21 05 2004 Model HUMACOUNT PLUS Serial No 2541 Version 2 9 Compiled 21 05 2004 PCPNIF Firmware version MPU Firmware version Self test results HCB dark HGB light Electr Voltage current offset Ampl test peak Self test results Noise test 1 pls 5 sec OK Atm 978 mBar OK Vacuum 218 mBar OK drift 3 10 OK Overall result Successful The first panel shows general information about the instrument See menu on the left The PCPNIF firmware version will always be empty the MP
22. MBE d ud ea eg 15 2 19 Omm e 5 15 241 Switcehlhg POWER 16 2 1218 ee 16 221 49 USB Primer POS uuu 16 21520 PON 16 2 2 Main mechanic and fluidic parts of the 17 2 2 1 M nee 18 2 2 222 na E 18 UND X Y uu u eee A 19 2 2 4 Volume Meter Tube HUMACOUNT PLUS only 20 2 2 5 icis LO za 20 22O STAI _ __ __ _ 21 2212 Valves 1 5 6 10 VIET aka 21 2 28 Washing 2 duo 21 22 9 ______6 _6_ _ ___ 22 Dee 22 2 3 OSIS AI CI a a SE 23 2 3 1 FUNMACOUNT PEUS Electronic BlOGK ne en O 23 2 3 2 HUMACOUNT PLUS Mechanic and Fluidic 24 233 HUMACOUNT EIEEHONIEBIOCK Ana 25 2 3 4 HUMACOUNT Mechanical and Fluidic 8 88888 akan 26 3 5 6 OPERATION OP THE SYSTEM L
23. Menu 5 2 1 Entering to Service Menu There is a Service menu for servicing and operation checking purposes The entry point is in the Maintenance Service screen where Service Information is displayed Enter the code to access Service menu 6484 5 2 2 Main Service Menu The Main Service menu provides access to submenus and service utilities Service menu Edit Service contact Device information Calibration Settings Testing Miscellaneous 5 2 3 Edit Service Contact Service information Your service partner You can edit the Information fields by keys Address external keyboard The information set up here is shown in the Maintenance Service screen for the user Phone Press the OK button if a field is completed Contact Person 5 2 4 Device Information In the Device Information menu the model name the serial Device information number the software version and compilation date appear Model HUMACOUNT PLUS Serial No 1359 Version 2 3 Compiled 21 12 2001 5 2 5 Service Calibration Calibration menu is provided for Service calibration purposes In result calculations the service calibration factors are used as the user calibration factors so they are multiplied for each 1 Calibration factors parameter 2 Apply user factors Calibration RBCoisp Factrec User Factggc Serv RBC measured If the user factor is near the bound 0 80 1 20 by setting the
24. Off In HUMACOUNT the washing process also use Cleaner solution to clean the chamber from the lyse reagent between the WBC and RBC measurements and after each measurement cycle High voltage burning pulses are applied to the apertures in each measurement cycle to prevent lipid and protein build up Service Manual HUMACOUNT amp HUMACOUNT PLUS 33 77 3 9 Cleaning process In HUMACOUNT PLUS the system can clean the apertures and the volume limiter with the connected cleaner reagent The pump aspirates the cleaner through the V7 On V3 Off and V9 On valves After that the instrument cleans the WBC chamber with cleaner reagent Section 6 4 The instrument generates vacuum in the WBC chamber with the pump and after that this vacuum aspirates the cleaner reagent into the WBC chamber through V9 On V3 Off V7 On V8 On This second process is called Autoclean see the figure which is a part of the normal cleaning cycle V11 DilWash V13 DilNeedle 2 d TN DilFloat DILUENT vom sen 4 1 PT ez 10 DilWbc V7 FloatUp V3 FloatDown V8 AspWbc IA m CLEANER Ver Micro Pressure E x 1 DrainMix V4 DrainWbc x ___ ___ eservoir u T n HUMACOUNT the pump aspirates the cleaner through the V5 On V15 On V2 On and V4 valves to clean the aperture After that the instrument cleans the chamber with cleaner reagent that remained in the tubes between V12 V5 a
25. PICO CEO TINGAN USO Ras Ines aa SNIGINOS 08068 SH HHZSUT 330155344 xx ag TOM ALIAILISNIS ANG OITWISMI 208 HO TBT Eg HOIIGSM3dMOO BETPHT F 4 4 4 g g 5208 dagua oem SOISUDISQ dgrn6rd uHZSUT do OIA PEA RT Service Manual HUMACOUNT amp HUMACOUNT PLUS 76 77 N Human 9 13 Electronic part PCBs High Voltage Board JPH iatron CI le lt gt Diatron 1 2 Qi E 8 8 5 2 8 PCPRO V1 7 _ Diatron LTD 9 14 Revision History Service Manual amp HUMACOUNT PLUS 77 77 Human Gesellschaft f r Biochemica und Diagnostica mbH Max Planck Ring 21 e D 65205 Wiesbaden Germany Telefon 49 6122 9988 0 Telefax 49 6122 9988 100 eMail human human de Internet http www human de 01 2004 06
26. The PIC makes displaying even greeting screen at start up and keyboard scanning after the loading the configuration into FPGA CSMO Connection to Horizontal motor CSV1 Connection to 1 5 valves CSM1 Connection to Vertical motor CSV2 Connection to 6 10 valves CSM 2 Connection to Dilutor motor CSV3 Connection to 11 16 valves Valve 16 PUMP CSK Connection to keypad L C G D G G O G O GQ C C 0 T i w HAHAHAH HAUL a nmm 1283 id mimi CSP Connection to the liquid CSD Connection to CSA1 CSA2 Connection amp pressure sensor board the LCD module to the amplifier 10 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 1 6 Safe configuration E PROM board IDEPROM This board contains 24FC256 serial E PROM which stores the FPGA s configuration data Safe greeting screen and identity information about the instrument Keeping the hardware identity information OEM information model version HUMACOUNT PLUS HUMACOUNT the IDEPROM makes possible to run the correct software HUMACOUNT PLUS or HUMACOUNT and store the instrument s serial number hon iD M The IDEPROM is connected to the MPNIF card and it is write protected Ja VIDEPROM 1 2 ae The data of the normal configuration E PROM on the MPNIF card can be modified by
27. by selecting Load oetup Defaults and starting all over again 6 5 DOSFunctions on the Instrument To check the main board as a PC motherboard or for other service actions you may wish to run the instrument like a normal PC In this case an MS DOS 6 22 operating system boot floppy disk is required Follow the steps below to boot from an MS DOS diskette e Connect an external PS 2 keyboard e After turning on immediately press repeatedly F2 on the external keyboard until you see the keyboard lights flashing on or you hear beeps Switch to mode by and and press and cursor keys on the keypad to stabilize screen e Use arrows on the keyboard to go to the BOOT section of the menu and go down to Boot device priority menu press Enter e The list shows the boot order move the cursor to the item you wish to change then press on keyboard to move it higher lower in the list Move Removable devices first e Press Esc key until you get to the Exit menu and press Save changes then Enter to confirm e Putthe MS DOS boot floppy into the floppy disk drive The instrument will boot from the floppy and after pressing Enter to accept date time you can use it as a DOS computer where floppy is A or B and SanDisk Hard Disk is C Service Manual HUMACOUNT amp HUMACOUNT PLUS ATITT This mode gives facilities to the Service Personnel to run DOS functions Scandisk for checking the Hard Disk the ma
28. cell counter probes The chamber on the left is an HUMACOUNT PLUS MIX chamber it is closed from the front and it is open at the top The chamber on the right is an HUMACOUNT PLUS WBC chamber with the hole in the front for the cellcounter probe It is closed at the top and it has the special WBC long PTFE draining connector Place for cellcounter probe Long PTFE draining connector In the HUMACOUNT there is only one chamber for diluting and measuring the blood cells This chamber is the same as the WBC chamber in the HUMACOUNT PLUS with the long draining connector but it is open at the top and it has an extender cap on it It is extremely important for both instruments that these chambers should have no damage or crack because it could cause erroneous measurements noise 2 2 Valves 1 5 6 10 11 15 There are two kind of pneumatic valves in the system 2 way Valve e 3 way Valve Valves are assembled in a unit of five on the valve blocks HUMACOUNT PLUS contains 3 of these boards 15 valves HUMACOUNT has only 2 valve blocks 10 valves 2 2 8 Washing head Washing head is located at the bottom of the H amp V moving unit and it is for cleaning the outer surface of the aspirating tip This washing process is made by diluent reagent and the fluid is drained by the pump The arrows on the picture show the direction of diluent flow Draining by the pump Diluent reagent It is recommended to check t
29. corresponding service factor the user factor can be adjusted to 1 00 40 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human Factppc user 1 19 and Factppc sen 1 00 and Factrec user 1 00 and serv 1 19 gives the same result for RBC Apply user factors function can be used to move the combination of the existing user and service factors automatically to the Service level If all service calibration factors were 1 00 after this function all service factors will be set to the product of the previous user and service factors and all user factors will become 1 00 5 2 6 Software Settings Software settings can be customized in this menu Autoclean cycle HUMACOUNT PLUS only after how many ettings measuring cycles HUMACOUNT PLUS makes an autoclean Autoclean cycle 0 50 process default 20 cycles Zero means never Standby time 10 60 min Lower this setting if periodic noise appears in the system i e 12 15 measurements good and after that 5 8 measurements with Serial I O speed Baud high PLT Serial Protocol Version 2 20 Stanby time after how many minutes the instrument goes to Waste container capacity 1 60 18 standby mode This is the setting used in Measure mode if Start Laboratory header lines has not been pressed chambers will be primed to avoid clogging 4 of aperture default 15 Rinse Time 0 3 hours Reblank time after how many hours
30. easy to access the mechanic and fluidic system Vy Reagent Sensors H amp V Moving Unit Valve Block Il Measuring Block Dilutor Block x m E L p K pi i Etem p j i j i 4 _ E i t m Rum 2 coc Mem eM m Peristaltic Pump Reagent Inputs 26 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 3 OPERATION OF THE FLUIDIC SYSTEM This section describes the main fluidic steps of HUMACOUNT PLUS and HUMACOUNT measurement cycle The fluidic systems are totally different in the two instruments but they use the same modules valves tubes chambers dilutor etc and from the point of the measurements they follow the same steps For this reason we show the fluidic system processes for both instruments in the same logical blocks HUMACOUNT PLUS and HUMACOUNT Fluidic Schematics are shown in Section 2 2 of this manual The following figures show the actual process and help to understand how the fluidic system works The following steps are introduced in this section Sampling process Aspirating tip washing process Diluting process Lysing process WBC counting process RBC counting process Washing processes Cleaning process DEN DE The active pipe is filled with black color beside the tube an arrow gt shows the direction of the flow
31. ie 27 3 1 27 3 2 SAMPING c A 28 3 3 ASPIFAUAgNID WashIng process nn nee A 29 3 4 PUUUNO D OCO 30 3 5 31 30 BGC ROCESS ni 32 3 7 KAC Gao a pun 33 3 8 33 3 9 Cleaning GARO 34 ADJUSTMENT snae ee eee ec ee 35 4 1 Mechanical 56 35 4 1 1 lO ee ee 35 4 1 2 ASPIaUNg ebene 36 4 2 S 36 4 2 1 Adjustment of the liquid detector 36 4 2 2 Amplifier offset setting AA AA D E 37 CHECKING OF PROPER OPERATION AA ee gen 38 5 1 TOS MAA AR DA PAPA PARAN d iN 38 5 1 1 SEN IRRE 38 9 1 2 JNormmaalrange or Sell test Paramelels naar u 39 5 1 3 Troubleshooting Guide for Self test rr e 39 5 2 SEIVICE
32. instrument makes the second dilution into the chamber after these processes Sample diluent V11 DilWash RES DILUENT CLEANER Nr 13 DilNeedle 2 1 V14 LyseWbc 4 Diluent amp Cleaner Sensor 9 Pressure Meter LYSE Puffer Reservoir 1 V4 DrainPuffer 30 77 Service Manual HUMACOUNT 8 HUMACOUNT PLUS Human 3 5 Lysing process In HUMACOUNT PLUS during this step the primary dilution remaining in the mixing chamber flows via V1 On and V4 On into WBC chamber because the vacuum which is generated by the pump aspirates the fluid through V8 On while V3 and V7 are Off Simultaneously 0 7 ml of lysing reagent is added through V14 On while the Lyse syringe moves upwards This process makes the 1 190 dilution rate and good mixing with lyse and at the end the mixing chamber is empty and the specified RBC and WBC dilutions are in the proper chambers After this step the system makes a MIX chamber washing cycle V11 DilWash V13 DilNeedle 2 1 puer el l i CLEANER o iL Ver Pressure Meter Puffer V1 DrainMix 4 Se 24 Reservoir ar T sa 36 E n T Waste Full WASTE gis In HUMACOUNT in this step 0 7 ml of lysing reagent is added into the measuring chamber through V14 On while the Lyse syringe moves upwards This process makes the 1 190 WBC diluti
33. lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 60 lt gt Receiver acknowledges transmission and requests for WBC package lt ACK gt WC 66 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human HUMACOUNT PLUS HUMACOUNT sends WBC package lt SOH gt DW lt STX gt SNO lt HT gt 1 lt LF gt DATE lt HT gt 20010405 lt LF gt TIME lt HT gt 163800 lt LF gt SID lt HT gt 1 lt LF gt PID lt HT gt 57290 lt LF gt CHN lt HT gt 256 lt LF gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt O lt HT gt 255 lt HT gt 25 5 lt gt 255 lt gt 251 lt gt 209 lt gt 163 lt gt 118 lt gt 82 lt gt 56 lt gt 40 lt gt 32 lt gt 29 lt gt 29 lt gt 30 lt gt 3 4 lt gt 40 lt gt 47 lt gt 55 lt gt 64 lt gt 76 lt gt 88 lt gt 103 lt gt 119 lt gt 136 lt gt 155 lt gt 174 lt gt 195 lt gt
34. lt gt 248 lt gt 251 lt gt 252 lt gt 253 lt gt 2 53 lt gt 253 lt gt 253 lt gt 253 lt gt 254 lt gt 255 lt gt 254 lt gt 252 lt gt 248 lt gt 243 lt gt 236 lt gt 229 lt gt 221 lt gt 214 lt gt 207 lt gt 199 lt gt 192 lt gt 185 lt gt 178 lt gt 170 lt gt 162 lt gt 154 lt gt 146 lt gt 137 lt gt 129 lt gt 121 lt gt 113 lt gt 104 lt gt 96 lt gt 88 lt gt 82 lt gt 75 lt gt 71 lt gt 66 lt gt 62 lt gt 58 lt gt 55 lt gt 51 lt gt 47 lt gt 43 lt gt 38 lt gt 35 lt gt 31 lt gt 28 lt gt 25 lt gt 22 lt gt 19 lt gt 18 lt gt 16 lt gt 14 lt gt 12 lt 1 gt 11 lt gt 10 lt gt 9 lt gt 9 lt gt 9 lt gt 9 lt gt 8 lt gt 8 lt gt 7 lt gt 7 lt gt 6 lt gt 6 lt gt 5 lt gt 5 lt gt 5 lt gt 4 lt gt 4 lt gt lt gt lt gt lt gt 2 lt gt 2 lt gt 2 lt gt 1 lt gt 1 lt gt 1 lt gt 1 lt gt 1 lt gt 1 lt gt 1 lt gt 2 lt gt 2 lt gt 2 lt gt 2 lt gt 2 lt gt 2 lt gt 2 lt gt 1 lt gt 1 lt gt 1 lt gt 1 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0 lt gt 0
35. open a new tank RBC PLT limit The RBC PLT valley is too high It is rather a diagnostic flag If the warning discriminator is in a wrong place in the PLT or RBC histogram then repeat the measurement for a correct PLT result RBC peak warning Perform cleaning and repeat the measurement clogging If itis a general problem change the RBC aperture RBC PLT coincidence The same action as in case of the M warning flag is too high Linearity error RBC PLT data The same action as in case of the D warning flag package errors RBC PLT time error The same action as in case of the D warning flag RBC PLT clogging The same action as in case of the C warning flag o Service Manual HUMACOUNT amp HUMACOUNT PLUS 61 77 9 2 Serial Communication Protocol V2 x 9 2 1 General Description The HUMACOUNT PLUS HUMACOUNT software is able to make serial connection link to a receiver device e g computer which is connected to the serial port If the Baud Rate setting in the Service Menu is set to a valid value other than Not Connected the instrument will try to initiate a communication sequence The serial communication is always initiated by HUMACOUNT PLUS HUMACOUNT and the receiver must respond to this The hardware protocol 8 data bit 1 stop bit no parity If the other side is not responding in 1 second the instrument will repeat the transmission twice but if still no response the other side will
36. than 1 Volt If the mains outlet does not provide a good ground you can make it by connecting HUMACOUNT PLUS HUMACOUNT to a grounded conductor i e water tap or other grounded piping If itis possible find another place for HUMACOUNT PLUS HUMACOUNT which is not near the other instruments Use your analyzer with an individual outlet or use line filters You should lower the Autoclean setting to 10 52 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 6 8 Possible Causes of Noise Generally high count of any particle even if you think it should be low or near zero can be caused by NOISE i e something interferes with measurement The most important thing in these cases to identify the source of the NOISE otherwise we cannot protect the system against it NOISE can come from has several sources and the different NOISE sources are added Sometimes we have to fight one of them but sometimes more Only one of them is enough to make problem 6 8 4 Contaminated reagent The most probable cause real particles are in the reagent and therefore the PLT blank is continuously high e g always 30 40 You can easily sort out this case by replacing diluent by opening a new tank PLT blank must go down is several blank measurements below 10 Another reason which is HUMACOUNT specific the PVC tube between the T fitting coming from nozzle 2 of Valve 5 and nozzle 2 of Valve 12 should have a mi
37. tip does not move in the total length just a part of it Some procedures perform this kind of aspirating tip washing aspirating tip up V11 DilWash E V13 DilNeedle et 5 an V12 DilFloat cT DILUENT IN V9 Cleaner 7 1 2 X NB vio DilWbc V3 Ok Al CLEANER Micro Pressure Meter _ X V1 DrainMix lt 4 DrainWbc X x V5 Puffer X V2 DrainRbc T e Waste Full Sensor WASTE Puffer Reservoir V6 DrainWash X Pump aspirating tip up 11 DilWash V13 DilNeedle Diluent VA gt gt x lt gt V12 DilChamber DILUENT V14 LyseWbc ET Diluent amp Cleaner V5 Cleaner Sensor Alle je CLEANER P Pressure Meter LYSE Puffer Reservoir 1 DrainChamber DE X V2 DrainAperture d WASTE In HUMACOUNT PLUS the Macro syringe doses the diluent through V13 Off V12 Off V11 On The Pump aspirates the diluent from the washing head through V6 On while the M2 Vertical motor moves the In HUMACOUNT the Macro syringe pushes the diluent through V13 Off V12 Off V11 On The Pump aspirates the diluent from the washing head through V4 Off while the M2 Vertical motor moves the Service Manual HUMACOUNT amp HUMACOUNT PLUS 29 77 3 4 Di
38. COUNT amp HUMACOUNT PLUS Human 15 0 08000 H HGB blank is high or no HGB blank 0x10000 No WBC three part 0x20000 WBC three part warning or WBC three part diff Unsuccessful The markers are given in histogram channel between 0 and 255 where 0 means that the marker could not be found 5 The parameter Ids in HUMACOUNT PLUS HUMACOUNT are P01 WBC 1071 P02 10 HGB g l P04 9e P05 fl P06 P07 MCHC 08 PLT 101 P09 PCT 96 P10 MPV fl P11 PDWsd fl P12 P13 RDWsd fl P14 RDWov 96 P15 LYM 1071 P16 MID 1071 P17 GRA 1071 P18 LYM 96 P19 MID 96 P20 GRA 90 P21 RBCtime sec P22 WBCtime sec parameter value is always 4 character wide spaces from left added if necessary It can 9999 if the value could not be displayed in 4 digits or if the value could not be calculated because of an error The meaning of the parameter flags Flag Displayed 0 Value correct Value high more than upper limit 22 f Value low less than lower limit E Value is unreliable Value not given because of error Value is Lu Value cannot be calculated There is no value I RBC WBC and PLT package The format of the histogram packages are the same they differ in type descriptor only The package contains the sample data to identify the package and the hi
39. D display as it is shown in the figure below HUMACOUNT keyboard panel 2 1 10 Start key amp glowing LED The Start key is a micro switch connected to the MPNIF card through the K amp L ribbon cable The glowing LED indicates the actual status of the analyzer and it has three colors red green and amber See User s Manual The LED has three pins and the actual color depends on the controlled pins The LED is also connected to the MPNIF card through the K amp L ribbon cable 2 1 11 Pressure amp Liquid Sensor Board PCPRO This board contains MPX5100AP calibrated pressure sensor which can measure the pressure of air It has two liquid detectors for sensing diluent cleaner and lyse reagents There are two potentiometers to adjust the sensitivity of both liquid sensors but in different position for HUMACOUNT PLUS and HUMACOUNT see the pictures below The potentiometer on the left is for the Diluent Cleaner the one on the right is for the Lyse detector sensitivity The PCPRO board is connected to the MPNIF card through the P ribbon cable On the other side of the board there are two liquid detectors with indicator LEDs which show the actual state of the sensors the corresponding LED is on if no reagent in the tube HUMACOUNT PLUS amp HUMACOUNT PCPRO Potentiometers amp Pressure sensor Doe pe eta FROS HEROS 004 1005 004 003 RO2 5 RO1 RO2 EQ NV S
40. HT gt 35 lt gt 37 lt gt 38 lt gt 41 lt gt 43 lt gt 46 lt gt 47 lt gt 48 lt gt 50 lt gt 52 lt gt 56 lt gt 60 lt gt 64 lt gt 70 lt gt 74 lt gt 79 lt gt 82 lt gt 86 lt gt 89 lt gt 92 lt gt 95 lt gt 99 lt gt 102 lt gt 103 lt gt 104 lt gt 105 lt gt 105 lt gt 106 lt gt 108 lt gt 110 lt gt 112 lt gt 115 lt gt 117 lt gt 119 lt gt 120 lt gt 121 lt gt 120 lt gt 120 lt gt 120 lt gt 121 lt gt 122 lt gt 125 lt gt 129 lt gt 132 lt gt 136 lt gt 139 lt gt 141 lt gt 142 lt gt 142 lt gt 140 lt gt 13 8 lt gt 135 lt gt 132 lt gt 129 lt gt 127 lt gt 125 lt gt 123 lt gt 122 lt gt 120 lt gt 117 lt gt 115 lt gt 112 lt gt 1 09 lt gt 108 lt gt 108 lt gt 109 lt gt 110 lt gt 110 lt gt 108 lt gt 104 lt gt 99 lt gt 94 lt gt 89 lt gt 85 lt gt 82 lt gt 80 lt gt 79 lt gt 78 lt gt 77 lt gt 75 lt gt 72 lt gt 68 lt gt 63 lt gt 58 lt gt 54 lt gt 50 lt gt 47 lt gt 44 lt gt 43 lt gt 41 lt gt 41 lt gt 42 lt gt 42 lt gt 42 lt gt 42 lt gt 39 lt gt 37 lt gt 34 lt gt 30 lt gt 28 lt gt 25 lt gt 24 lt gt 2
41. HUMACOUNTPLUS A400 Meas Block excl Amplifier HUMACOUNTPLUS 401 56 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS CODE A403 A404 A405 A410 A420 A421 A422 A430 A431 A433 A434 A440 A441 A442 A500 A501 A502 A503 A504 A505 A506 A507 A508 A510 A511 A530 A531 A532 A541 A542 A543 A544 A545 A546 A547 A548 A549 NAME Human HUMACOUNT HUMACOUNT PLUS Service M anual HUMACOUNT amp HUMACOUNT PLUS 57 77 CODE NAME HUMACOUNT HUMACOUNT PLUS A550 Diluent cont connector Divent cont connector _ ___ A551 colour locking ring A552 Colour lock _ A554 568 Reagent tubing set HUMACOUNT PLUS A559 Reagent tubing set HUMACOUNT 050 A600 Body HUMACOUNT PLUS mounted A611 Sampling bar HUMACOUNT PLUS mounted A612 Gloving LED cover HUMACOUNT PLUS plaste A613 Gloving LED cover board compete _ ___ A620 Instrument door HUMACOUNT PLUS lp A630 Upper body cover HUMACOUNT PLUS plastic A660 HUMACOUNT PLUS cover with HUMAN oo A661 HUMACOUNT PLUS cover withoutlogo 0 A662 HUMACOUNT cover with HUMANIogo L A663 HUMACOUNT cover withoutlogo o ___ 712 Kmotorcable HUMACOUNTPLUS lp A713 Ymotorcable HUMACOUNTPLUS S A714 Pilutor cable HUMACOUNT PLUS lp A715 Keyboard cable HUMACOUNT PLUS A716 LCD cable HUMACOUNTPLUS A717 1 5 cable HUMACOUNT PLUS
42. LF gt NAME lt HT gt lt LF gt BIRTH lt HT gt 00000000 lt LF gt SEX lt HT gt O lt LF gt DOC lt HT gt lt LF gt OPID lt HT gt 0O lt LF gt MODES lt HT gt O lt LF gt WRN lt HT gt O lt LF gt PM1 lt HT gt 10 lt LF gt PM2 lt HT gt 135 lt LF gt RM1 lt HT gt 34 lt LF gt WMI lt HT gt 21 lt LF gt WM2 lt HT gt 55 lt LF gt WMS3 lt HT gt 91 lt LF gt PARN lt HT gt 22 lt LF gt PO1 lt HT gt 6 4 lt HT gt 0O lt LF gt PO2 lt HT gt 4 36 lt HT gt O lt LF gt PO3 lt HT gt 131 lt HT gt 0 lt LF gt P04 lt HT gt 39 0 lt HT gt 0 lt LF gt P05 lt HT gt 90 lt HT gt 0 lt LF gt PO6 lt HI gt 30 0 lt HT gt 0 lt LF gt P0 7 lt HT gt 335 lt HT gt 0 lt LF gt P 08 lt HT gt 262 lt HT gt O lt LF gt POY lt HT gt 0 24 lt HT gt 0O lt LF gt PIO lt HT gt 9 2 lt HT gt 0O lt LF gt P11 lt HT gt 12 0 lt HT gt 0O lt LF gt P12 lt HT gt 3 6 9 lt HT gt 0 lt LF gt P13 lt HT gt 52 3 lt HT gt 1 lt LF gt PI4 lt HT gt 16 3 lt HT gt O lt LF gt PI5 lt HT gt 2 1 lt HT gt O lt LF gt PI6 lt HT gt 0 5 lt HT gt O lt LF gt P17 lt HT gt 3 7 lt HT gt O lt LF gt PI8 lt HT gt 33 4 lt HT gt 0O lt LF gt PI9 lt HT gt 7 8 lt HT gt 1 lt LF gt P20 lt HT gt 58 3 lt HT gt 0O lt LF gt P21 HT 8 4 lt gt 0 lt gt 22 lt gt 5 0 lt HT gt 0O lt LF gt lt ETX gt 89 lt EOT gt Receiver acknowledges DATA transmission and requests for RBC package lt ACK gt RB HUMACOUNT PLUS HUMACOUNT sends RBC package lt SOH gt CR lt SSTX gt SNO lt HT gt 1 lt LF gt DATE lt HT gt 20010405 lt LF gt TIME lt HT gt 163800 lt LF gt SID lt HT gt 1
43. Measurement amp Pneumatic Interface Card MPNIF MPNIF card incorporates measurement processing and also the pneumatic display and keyboard functions in one board In addition this card has an MDA monochrome display adapter emulation mode which allows the Service Person to use the LCD like a computer monitor or checking the proper operation of the instrument s main board The controller card is connected to the main board through the ISA bus After power on the card holds the main board in wait state with IOCHRDY signal until the PIC micro controller configures the FPGA circuit from the configuration E PROM located on the card or from the safe located on the IDEPROM board After that the FPGA makes and memory address decoding and selects the actual part placed on the board e Video RAM for MDA Monochrome Display Adapter emulation and Graphics controller PIC e Motor controller PICs micro controllers controlling stepper motors The FPGA circuit also performs the main measurement data sampling and communication tasks The board s AID chip with its own sample hold circuit makes the analog to digital conversion After that the FPGA makes the data processing and stores the results in a temporary FIFO memory The results are sent to the PC main board by DMA cycles With the internal registers of the FPGA the software can control the valves the digital interface of the amplifier and the controller PIC
44. PS uh P ES F lt US n tt a ARRAREN 1 2 5HIELD ABCABL12 5CH ea E z c E LiLELELIELILI 4 REEEEEGEERPEEFER ABACUS CABLING DIAGRAM Size Document B d MAA EE PAGEL BOOB pd ba HI LT s i e e B P Lh p an a Wm _ 6 Arme a VALVE V1 2 A BOARD was TAAS RR RR RR Rei KA newer a a KEN NAA AA EBZGGIEHSGSGERESATERRMUERRUU EM e RR LIS J MEI in EIE T IDEEPROM an ag a m MATRIX VALVE BOARD VALVE V1 2 Poe Pa AMAN A d START KEN 1 COLD CATHODE LAMP CONNECTOR Lu dM TAQOPLEC LIGIUD PRESSURE ROBRD aT DOO n MEASWREMENT amp PNEUMATIC TNTEREACE BOARD a an 4 rentes 5 TCOTER D WW T DLL CLE E JanunaananaBa 1 5
45. Puffer Reservoir V4 On a DrainMix XS V6 DrainWash X e V5 Puffer gt X v2 DrainRbc n HUMACOUNT the regulated vacuum it is generated by the pump in the puffer reservoir aspirates the diluted sample WBC from the chamber through V2 On valve There is no volume limiter so the instrument counts the cells for 8 seconds At the end of this process the HGB measurement is executed V11 DilWash V13 DilNeedle 1 DILUENT CLEANER Diluent amp Cleaner Sensor P Pressure Meter LYSE Puffer Reservoir 2 V1 DrainChamber x V2 DrainAperture 32 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 3 7 RBC counting process In HUMACOUNT PLUS the RBC counting process is executed immediately after the WBC counting process when the float is at the lower opto sensors The measuring vacuum which is in the puffer reservoir aspirates the RBC sample through V7 ON and V5 ON valves while the V3 valve is OFF Both the WBC and the RBC samples are flowing but the float measures only the RBC sample volume by moving upwards With this method a predefined sample volume 200 ul is measured but he WBC and the RBC measuring times are different because the diameter of the cell counter apertures are different V11 DilWash V13 DilNeedle 2 F Ek n vi 0 DilWbc el V7 FloatU V3 FloatDo 8 T CLEANER 4
46. Service Manual HUMACOUNT amp HUMACOUNT Plus Hematology Analyzer Revision 3 0 Cat No 16400 2 TABLE OF CONTENTS 1 2 INTRODUC TION ans nn 5 1 1 he ee 5 1 2 lir m T 5 1 3 Integrated softlware a 5 FONC HONAL DESCRIPTION zu en 6 2 1 Maim electronic pans ofthe 2 NANANA DARA BIAG ANA 6 2 1 1 GE E ee E I d EE 7 212 7 251522 Volume ore and 8 Ze 2 ee 8 2 1 5 Measurement amp Pneumatic Interface Card 10 2 1 6 Safe configuration E PROM board IDEPRON einen ee 11 2 1 7 Aigi Voltage Board AV een nennen 11 Graphic ECD MOQUE sr maaa uu quqa qa qu 12 2 1 9 13 21 10 Start Key 13 2 11 Pressure amp Liquid Sensor Board POPR00uu uu u barbare Ei 13 2 1 12 Stepper Motor Opto 14 2 119 Valve DAVer BOI e u Z uuu ete tuc 14 Mat Board
47. U firmware version holds the MPNIF version number The second and third panel contains tested parameters as follow HGB dark count HGB LED is off this parameter is always zero the HUMACOUNT and light LED is on Electrode voltage measuring current and amplifier offset Amplifier by generating 20000 test pulses including gain related peak value noise related deviation Amplifier Noise test during a 5 second period Atmospheric pressure stands for outer pressure Vacuum reports pump operation vacuum generated by the pump in a 10 second period of time Drift represents pressure loss of vacuum measured in a 10 second period of time At the end of each result line a status message is displayed which means that the actual test result is in the normal range OK higher HIGH or lower LOW than the pre determined limits or the result produced error ERROR Overall test result is displayed which can be Successful in case of every test result is OK or Errors if any of the parameters is HIGH LOW ERROR 38 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 5 1 2 Normal range of Self test parameters Parameter Une Lower bound Upper bound com 0 3000 deviation noise mwv 0 Nose O _ Suter pressure mBa 600 UU on mp o 19 5 1 3 Troubleshooting Guide for Self test Parameter Mak
48. am packages The histogram package can be RBC WBC and PLT Their format is the same the type descriptor differs only 9 2 5 INIT package HUMACOUNT PLUS HUMACOUNT sends the general identifiers by this package during initiation of a communication DEVICE lt HT gt VERSION lt HT gt DATE lt HT gt TIME DEVICE device identifier VERSION version of the software e g 2 7 DATE date in YYYYMMDD format TIME time in HHMMSS format Service Manual HUMACOUNT amp HUMACOUNT PLUS 63 77 9 2 6 DATA package This package contains sample and patient data the measured parameters and the markers of histograms One data element consists of two items name and value which are separated by a lt HT gt character and closed by lt LF gt Important SNO DATE TIME SID and PID fields will always be present in this order but other fields may be omitted SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 1AXX435 lt LF gt PID lt HT gt B72D44 lt LF gt NAME lt HT gt JOE SMITH lt LF gt BIRTH lt HT gt 19650208 lt LF gt SEX lt HT gt 0 lt LF gt DOC lt HT gt Dr John Gold lt LF gt OPID lt HT gt 1172 lt LF gt MODE lt HT gt 0 lt LF gt WRN lt HT gt 0 lt LF gt PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LF gt WM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 lt LF gt PO1 lt HT gt 6 6 lt HT gt 0 lt LF gt PO2 lt
49. annot write archive data Check disk It Cannot write archive may be unformatted data because some Replace the floppy disk Replace the Cleaner reagent container with a filled one full or bad Do you want disk errors to retry Cannot read archive Archive disk is data damaged or not an Change the floppy disk archive Do you want to retry archive disk Cannot load archive Disk is not an archive data disk or damaged Change the floppy disk archive Check the connections between the instrument and the printer and check the printer setup in the Settings Printer settings sub menu Feed the printer with paper Check the connections between the instrument and the printer and check the printer setup in Printer error d Printer error Printer is out of paper and there are some other printer errors Printer is out of paper and error d the Settings Printer settings sub menu Printer is out of paper Printer is out of paper Feed the printer with paper The selected printer type does not match to the printer TO Paper width steps over Modify the paper setup in the Settings Printer settings sub menu Printer was not set up correctly Modify the printer setup in the Settings Printer settings sub menu Service Manual HUMACOUNT amp HUMACOUNT PLUS 49 77 Code Ewortext Reason Remeay Paper height is too Paper height steps over Modify the paper setup in the large the margin S
50. art Possibly lyse problem but in some pathological samples too high lymphocytes it can be happened HGB blank is high gt Repeat the blank measurement and accept it no HGB blank Possibly lyse or diluent problem WBC blank is high gt Repeat the blank measurement and accept it no WBC blank Possibly lyse or diluent or noise problem L WBC RBC limit Check the 1 RBC LYM discriminator If it is in the minimum point or warning close to it accept the results Otherwise repeat the measurement If the retried action makes very similar results and discriminator is in a wrong place then the MID and results are OK but the WBC and LYM results can be higher because of the RBCs Too many RBC cut Repeat the measurement Possibly lyse problem from WBC If the WBC measuring time is too high more than 8 sec it could be aperture clogging In that case perform cleaning and repeat the measurement WBC coincidence is The results are out of the linearity range Make a dilution with an external too high Linearity dilutor with a pre defined dilution range Do not forget to correct the error results with the defined factor WBC data package Perform cleaning and repeat the measurement aperture clogging PLT blank is high or gt Repeat the blank measurement and accept it RBC blank is high Repeat the blank measurement and accept it no RBC blank Diluent problem Replace diluent
51. be supposed to be not ready to receive data and thus HUMACOUNT PLUS HUMACOUNT will not try to communicate any more From this state it can wake up by receiving an lt ENQ gt ASCII code 5 character from the other side and the instrument will immediately respond an lt ACK gt ASCII code 6 From this point the instrument will send data if it is ready for transmission The communication is based on packages There are 5 package types INIT package Device identification software version current date and time DATA package Sample and patient information measured parameters and markers e RBC package Sample information RBC histogram e WBC package Sample information WBC histogram PLT package Sample information PLT histogram The communication sequence is always started with an INIT package If the link is successful the instrument will send DATA package and the receiver can request RBC WBC and PLT packages at acknowledge opecial characters used in the communication me 2 _ me 3 op 62 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 9 2 2 Format of Packages Sent by HUMACOUNT PLUS HUMACOUNT The packages sent by HUMACOUNT PLUS HUMACOUNT are always between a lt SOH gt and lt EOT gt character and they consist of header data and tail The header consists of a package identifier and a package type descriptor The tail includes the checksum Typical format l
52. ble floppy disk in the drive and boot order is set to start on floppy disk and the system is waiting for user confirmation Remove disk and press a key If you need to run BIOS setup an external keyboard must be connected Hold the button and press and release the button on the foil keypad of the analyzer to activate the mode Use the like a shift button The display will use the LCD like a primary computer monitor 80 characters by 25 rows The LCD is smaller than the standard monitor area therefore just about a quarter of the total screen is displayed at once 40 characters by 16 rows Service Manual HUMACOUNT amp HUMACOUNT PLUS 45 77 to change the actual quarter displayed use cursor keys lt 1 on the keypad to go where the cursor is cursor follow operation press the 2 button to go back to the normal graphic displaying mode press again Quarter 1 on LCD Quarter 2 on LCD Quarter 3 on LCD Quarter 4 on LCD Definition of displayed LCD quarters in MDA emulation mode The MDA screen and the Graphical Application Display are existing in the same time They use different area of the video RAM located on the MPNIF board Displaying is controlled by the configuration PIC on the MPNIF board and hosted by the FPGA If there is no display in MDA mode or garbage characters are displayed check the following points 1 board for correct conn
53. ecified it can be given in years or months Predilution ratio can be selected between 1 10 or 1 3 This ratio is used in Pre dilution mode Reagent code a value to modify the zero point of HGB function valid between 0 30 default 9 For pre diluted sample HGB can have a significant offset due to reagent non linearity LCD light off delay hrs 0 Set code 0 and measure HGB in pre diluted mode and in normal mode Adjust the code until the 2 HGB results are the same then calibrate HGB Use this reagent code with your reagents Extend Probe Voltages This option is ON by default It makes the instrument accept the physically highest probe voltages 450 on both channels it is expressed in A D resolution where 1023 shows 55V so 450 is practically 24 2 V on the electrode Probe Voltage displayed in the measurement results screen are representing the electrical probe potential of the measuring electrode They are displayed in a range like RBC Probe Voltage RPrV 223 228 WBC Probe Voltage WPrV 245 249 Settings Predilution Ratio 1 3 Reagent code 9 Extend ProbeVoltages Calendar Mode Gregorian Probe voltage can change a maximum of 4 If it changes more than 4 or it is higher than 420 or 450 if Extend Probe Voltages in On the analyzer will display an error and C or c flag will show clogging condition on the corresponding channel Calendar mode You can select between the Gregorian and the Jalaali Persia
54. ection the ISA slot 2 DimmPC connector the CPU is not placed correctly in the socket or there is bad contact 3 Replace the DimmPC 6 4 Key BIOS settings for correct operation To start the setup utility keep repeatedly pressing the F2 key on the external keyboard during startup The start of the Setup program is indicated by the keyboard status LED s coming on and off then only NumLock remains on Additional beeps when F2 is pressed indicate running of the Setup program Now use dot keys on the instrument keypad to enter mode to see computer display on LCD With MDA mode you get the normal PC screen displayed on the LCD As the LCD is smaller than the screen only a part one quarter of the screen is shown You can move this display window of the screen using the arrows on the keypad If you press the displayed quarter will follow the cursor To select an item simply use the arrow key to move the cursor to the field you want Then use the plus and minus keys to select a value for that field The Save Value commands in the Exit Menu save the values currently displayed in all the menus To display sub menu use the arrow keys to move the cursor to the sub menu you want Then press Enter A pointer marks all sub menus 6 4 4 BIOS Settings of DimmPC Main menu Legacy Diskette 1 44 1 25 MB 7 Primary Master AUTO Primary Slave AUTO Advanced Menu Reset Configuration Data No Halt On Er
55. en a cell passes through the aperture it causes a change in resistance which generates a voltage pulse The amplitude of the voltage pulse is proportional to the ratio of cell volume per aperture volume This is used to determine the volume of cells The number of cells can be obtained by counting the pulses n the HUMACOUNT PLUS there are two Cellcounter Probes WBC probe with 100 um aperture and RBC probe with 80 um aperture Both have a ground electrode assembly and U shaped metal fixing as it is shown in the next figure n the HUMACOUNT there is only one Cellcounter Probe the aperture size is 80 um this is the same as the RBC probe in the HUMACOUNT PLUS Assembled Cellcounter Probe Measuring tubes Red rings mark EA 9 measuring tubes One RBC 80 um LL a Two WBC 100 um lt U shaped metal fixing Measuring tube Ground electrode assembly The aperture is made of ruby and it is moulded into the measuring tube The correct sealing around the aperture is very important for measurements 2 1 2 HGB Head Hemoglobin head is placed at the bottom of the WBC chamber in the HUMACOUNT PLUS and at the bottom of the measuring chamber in the HUMACOUNT It contains light source LED at 540 nm and Photo Detector TSL235 The Photo Detector converts the light to frequency The HGB concentration is a logarithmic function of this frequency measured by the FPGA circuit of the MPNIF card Co
56. en the instrument To lock the cover pull the fastener to the opposite direction e For HUMACOUNT there is no special fastener for the cover by unscrewing the cover fixing screws on the rear panel the cover is ready to open But this instrument has a special sliding rack system for the main board holder plate to provide easier access for cables By loosening the main board holder plate fixing screws on the rear panel the Service Q Personnel can pull out the entire main board holder plate with the main board from the front of the instrument and access the back of the assembly plate a Figure on the left shows a dismounted HUMACOUNT for easier understanding 26 44 77 Service Manual HUMACOUNT 8 HUMACOUNT PLUS Human 6 2 Removing Installing DimmPC 6 2 1 Removing DimmPC The DIMM PC can be dismounted in the following way see Figure below 1 Discharge all electricity from your body by touching the instrument case before proceeding DimmPC is very sensitive against electrostatic discharge Remove the safe lock screw M2 x 10 mm hexagonal 2 3 Open the two white levers on both sides so that Dimm PC flips up 4 Pull out DimmPC module from the socket in a sloping angle 2 Remove screw 3 Open levers Dismounting of DimmPC 6 2 2 Installing DimmPC 1 Discharge all electricity from your body by touching the instrument case before proceeding DimmPC is very sensitive against electrostatic discharge
57. erated by a small tag fixed on the moving part The principle is the interruption of infra red light Lyse syringe emitted by the diode of the sensor The control lights LEDs are showing the actual state of the sensors if the light is on the Macro syringe dilutor moving part reached the corresponding sensor Micro syringe HUMACOUNT PLUS HUMACOUNT dilutor module works with separated syringes glass for Macro and Lyse plexiglass for Micro Macro syringe is for diluent reagent Lyse syringe is for Iyse reagent and the Micro syringe is for precision sampling during Control lights measurement LEDs for chackin the state of opto switches Opto switches Maintenance should be provided to the piston tips by applying neutral silicon grease to the cogged end of the Macro and Lyse pistons between the syringe and the tip itself This will ensure optimum sealing and longer lifetime of piston tips Greasing of the cogged transmission parts cogwheel and cogged bar should be done regularly using machine grease It is recommended to check and repeat greasing of piston tips and transmission gear every year or after 10000 measurements 18 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 2 3 H amp V moving unit XY This unit is the same for both instruments HUMACOUNT PLUS and HUMACOUNT and it contains the Horizontal and Vertical stepper motors H amp V motor opto board
58. ervice Manual HUMACOUNT amp HUMACOUNT PLUS 53 77 6 8 4 Internal noise sources The most annoying but real cause is some sort of internal noise The reason for this phenomenon is that the inside electrode hot point of the measuring circuit must be well insulated from the surrounding electronics otherwise inside noise sources can take their effect A Bad chamber insulation bad shielding of the chamber floating shield couples signals to the chamber and does not prevent against them Check grounding of shield remove it and clean the surface between the shield and the metal base bad reference electrode connection floating ground reference Repair is required bad sealing of aperture Replacement of measuring tube is required broken measuring chamber starts to conduct through the gaps ground path Replacement of chamber is required contaminated draining tube starts to conduct due to protein or lipid build up It is very easy to identify this case After replacing the drain tube of the measuring chamber mainly WBC WBC histogram peak or PLT becomes low soon Normally a good cleaner is required to dissolve lipid or protein build up Sometimes the cleaner is not strong enough to keep this tube clean enough Periodic washing using 1 hand warm bleach solution helps B Bad insulation of electronic signal paths In these cases check for any capacitive coupling of electronic signals to the chamber interference with HGB head high
59. etectors of the H amp V moving unit The top of this block is called HV head and it is shown in the figure below Setthe distance to 2 3 mm between the two parts of the head 2 3 mm BERE Loose the screw for free movement on the timing belt WES TR Adjust the opto wheel to the home position while the head stays screws untouched Fix the screws screws Home position is reached when the HOME hole is in the HOME opto sensor and the HOME state LED goes OFF on the H amp V motor opto board see picture below After that check the END position as well move the needle to the lowest position it is indicated by END state LED In that state 1 2 mm distance must be between the carriage and the bottom of the H amp V moving unit HOME state LED END state LED END hole END opto sensor HOME hole HOME opto sensor OPTO wheel Service Manual HUMACOUNT amp HUMACOUNT PLUS 35 77 4 1 2 Aspirating tip setting This adjustment sets the aspirating tip to the operational position Set the distance between the two parts of the head to 5 mm Loose the screws and adjust the aspirating tip to its operational position le Aspirating tip Bottom plane of Teflon sealing ring washing head Tubes to from the Washing head Washing head is located in the bottom of the H amp V moving unit which is shown in the figure above Use the Needle setting menu poin
60. ettings Printer settings sub menu 1406 Paper width is too small 299 width steps over Modify the paper setup in the the margin Settings Printer settings sub menu 1407 Paper height is too Paper height steps over Modify the paper setup in the small the margin Settings Printer settings sub menu e Modify the margin setup in the 1408 Left margin is too large Left margin is too large Settings Printer settings sub menu Top margin is too large Top margin is too large Settings Printer settings sub menu The distance between Modify the vertical spacing setup in the 9 9 two results is too high Settings Printer settings submenu Check the connections between the in print instrument and the printer and check the et Printer software error printer setup in the Settings Printer settings initialization au call Try to make a software upgrade You have to add at Follow the instruction change to single least one user to use Multi user problem user mode in the User settings submenu multi user mode 50 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 6 6 2 Message code list Are you sure to prepare the device to delivery S00 Remove reagent tubing at rear reagent inputs Diluent Lyse and Cleaner 5002 Connect min 100 m water to reagent inputs using cleaning tubo Kt 8005 Remove cleaning tube Kit Keep reagenti
61. formation or from the Self test submenu Instrument software V1 7 and above is absolutely Plug and Play it can detect what type of instrument is used and it will control the analyzer according to the hardware without any user or service help Every HUMACOUNT PLUS HUMACOUNT software version can be updated by the latest release available from HUMAN Service Manual HUMACOUNT amp HUMACOUNT PLUS 5 77 2 FUNCTIONAL DESCRIPTION 2 1 Main electronic parts of the analyzers HUMACOUNT PLUS HUMACOUNT Hematology Analyzers consist of the following electronic parts 1 Cellcounter Probe s HGB Head Volume Opto Board only for HUMACOUNT PLUS Amplifier Board Measurement amp Pneumatic InterFace Card MPNIF Safe configuration E PROM board High Voltage Board Graphic LCD Display Module 9 Keyboard Panel 10 Start key amp glowing LED 11 Pressure amp Liquid Sensor Board 12 Stepper Motor Opto Boards 13 Valve Driver Boards 14 Switching Power Supply 15 MBA Main Board with DimmPC 16 Floppy Disk Drive 17 Parallel Port optional USB printer port 18 Serial Port EEE 9219 Start key Measurement amp Pneumatic Interface Display J ennt Controller MPNIF PIC Keyboard FPGA Safe Conf Interface configurator E PROM High Voltage Graphic LCD pa
62. frequency signal is coupled to the chamber HGB head metal parts must be grounded The ground comes externally it must be in place otherwise HGB head does not shield but couples in noise interference with internal high voltage inverter high frequency signal is coupled to the chamber Repair is required avoid near contact of HVB cable to chamber or shielded amplifier cable interference with internal start button polling signal to start button may cause noise Guide start button wires as far from chamber as possible You may try mix them up on the start micro switch if applicable interference with display cable high frequency LCD signal is coupled to the chamber by the ribbon cable Keep the ribbon cable far from the chamber interference with CPU fan or other digital logic traces CPU fan or other digital signal radiates to chamber or to the shielded amplifier cable Try keeping the ribbon cables far from the chamber and shielded cable C Bad components or connections bad soldering salt residuals or component failure on amplifier especially if some reagent could get in the amplifier section Cleaning of PCB electrode socket or replacement of amplifier is required Check for the correct soldering of reference cable and its connector MPNIF board bad soldering or component failure Check the shielded cable connections as well Sometimes inside out connection the hot electrode goes outside as a shield is the problem both ends of a
63. functioning properly F4 5 2 10 Stress mode In Stress mode the instrument performs measuring cycles without sample blank measurements continuously This can be used for burn in tests or to check clean pneumatic system after changing any main fluidic parts You can have information about stability cleanliness HGB operation and for HUMACOUNT PLUS the float operation Results of the last 10 PLT and HGB blank is displayed as well You can detect any kind of noise or bubbles in the system if the PLT is not stable low or HGB has big variation The operation of the volume limiter tube can be monitored by the displayed floating measuring time for RBC and WBC measurement and besides their calculated minimum maximum and the standard deviation To exit from this mode press START button at the end of a normal cycle until the Stress operation is finished 5 2 11 Miscellaneous settings In this menu you can directly perform Software upgrade this Miscellaneous will restart the instrument 1 Software upgrade Clear Device Statistics Device statistics number of 2 Clear device statisctics measurements aperture clogging and other errors can be 3 Clear database cleared 4 Offset adjustment 5 Needle setting 6 Log in as supervisor Clear Database The whole Database measurement results including histograms can be cleared Offset Adjustment Provides an easy way to set the offset value see Section 4 2 2 Enter this
64. he state of the washing head at regular service visits Clean replace it yearly or after 10 000 measurements Service Manual HUMACOUNT amp HUMACOUNT PLUS 21 77 2 2 9 Puffer reservoir The puffer reservoir insures the stable vacuum during the measuring process HUMACOUNT PLUS and HUMACOUNT puffer reservoirs are made by glass but in different sizes The figures of these units are shown on the assembled analyzers section of this manual 2 2 10 Tubes The fluidic system contains tubes which have different diameters and lengths and also made by different materials Reagent paths requiring perfect sealing are made of Tygon tubing the draining circuit is made of silicon The Tubing Schematics Section contains the parameters of the tubes length amp diameter It is recommended to check the state of the tubing especially lyse path regularly during service visits Run a complete bleaching cycle check or replace tubing and fittings yearly or after 10 000 measurements 22 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 3 Assembled Analyzers This section includes the photos of the assembled analyzers There are two main parts for both instruments e The electronic block located on the left side e The mechanic and fluidic block located on the right side and it is assembled to a stainless steal assembly plate which makes also the separation from the electronic block 2 3 1 HUMACOUNT PLUS Electro
65. he washing head and replace if necessary Perform correct adjustment of the needle height see Section 4 1 2 Checking and Replacing Peristaltic Pump Tube Replace peristaltic pump tube if needed You can check it by opening the lock and removing the tube for inspection Check for leakage of the tubing Reassemble the head Warning Be careful DO NOT twist the tube while reposition it into the head because it will cause malfunction in a very short period of time Checking of the Power Supply Open the instrument for regular cleaning inside Check the cleanliness and operation of the cooling fan of the power supply Clean or replace the fan if necessary Bleaching of Fluidic System It is recommended to run a bleaching procedure to remove stains from the fluidic system Connect 2 5 hand warm clean bleach solution to all reagent inputs and perform priming on all reagent inputs Leave it in the tubing for not more than 2 3 minutes Remove bleach and prime all on air Connect distilled water 100 ml and perform priming all reagents again Connect reagents and run priming again Service Manual HUMACOUNT amp HUMACOUNT PLUS 55 77 8 SPARE PARTS CODE NAME HUMACOUNT HUMACOUNT PLUS A130 Front panel HUMACOUNT PLUS complet A131 Front panel HUMACOUNT PLUS excl keyboard A132 Foil keyboard HUMACOUNT PLUS A140 Amplifier board HUMACOUNT PLUS complate 154 A196 MB4 motherboard holding plate
66. in board and the floppy disk drive Do not forget to set back Hard Disk first under boot order section in the way specified above otherwise Software Upgrade will not work and the system will hang if a floppy disk will be left in the floppy drive during start up 6 6 Error messages The analyzer checks the operation of several mechanic fluidic and electronic parts during measurement The system shows the type of the error on the LCD display if any kind of malfunction is detected The electronic parts have a very little chance to fail only the connections and cables could get disconnected which can cause malfunction of the electronic system The mechanic and fluidic system have more chance to go wrong because it has moving parts 6 6 1 Error code list jos filename concerned d error type concerned You error specific string emortem Fm Check the MPNIF card and DimmPC for Out of memory in s insertion program error Try to make a software upgrade ante Error d opening file Fatal system or a oS program error Cannot create file 9os p The same as in case of error 1000 program error Data file 9os is corrupt Stored data file is 155 New stored data will be created new will be created all corrupt or missing stored measure data will Disk or software automatically by User confirmation be lost error 0 Error d
67. inWash X WASTE Stepper Motor LDL Liquid Detector HUMACOUNT PLUS Fluidic Schematics HUMACOUNT Fluidic Schematics V11 DilWash V13 DilNeedle 2 1 V14 LyseWbc 1N 2 YX LDL Lyse Sensor Diluent amp Cleaner P Pressure Meter Puffer Reservoir Y V4 DrainPuffer gt 1 2 LDx Liquid Detector AXE 2 way Valve WASTE Closed Off Stepper Motor Open On Di CLEANER DILUENT 1 3 Off 2 3 lt L DILUENT CLEANER 1 3 Off 2 3 Service Manual HUMACOUNT amp HUMACOUNT PLUS 17 77 2 2 1 Aspirating tip The aspirating tip is assembled in the H amp V moving unit and it makes the sample aspirations The right setting of the aspirating tip is necessary and very important see Chapter Adjustments 2 2 2 Dilutor This unit is the same for both instruments HUMACOUNT PLUS and HUMACOUNT and includes the dilutor stepper motor the dilutor motor opto board three syringes and the piston rods with special transmission The dilutor motor opto board is on the other side of this unit The picture below shows the HUMACOUNT PLUS HUMACOUNT dilutor module with the three separated syringes m e m The dilutor block is operated by a stepper e motor which is connected to the motor opto board The motor opto board has home amp end position opto switches The optical home end position switches are op
68. lt LF gt PID lt HT gt 5 7290 lt LF gt CHN lt HT gt 256 lt LF gt 4 lt HT gt 9 lt HT gt 17 lt HT gt 27 lt HT gt 37 lt HT gt 48 lt HT gt 58 lt HT gt 66 lt HT gt 70 lt HT gt 72 lt HT gt 70 lt HT gt 66 lt HT gt 60 lt HT gt 54 lt HT gt 47 lt HT gt 39 lt HT gt 33 lt HT gt 28 lt HT gt 22 lt HT gt 18 lt HT gt 15 lt HT gt 11 lt HT gt 9 lt HT gt 8 lt HT gt 7 lt gt 5 lt gt 4 lt gt 4 lt gt lt gt lt gt 2 lt gt 2 lt gt 1 lt gt 1 lt gt lt gt lt gt lt gt lt gt lt gt lt gt 0 lt gt lt gt lt gt lt gt lt gt 0 lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt lt gt 1 lt gt 1 lt gt 1 lt gt 2 lt gt 2 lt gt lt gt 4 lt gt 5 lt gt lt gt lt gt 8 lt gt lt gt 10 lt gt 11 lt gt 12 lt gt 14 lt gt 16 lt gt 18 lt gt 21 lt gt 24 lt gt 28 lt gt 31 lt gt 35 lt gt 38 lt gt 43 lt gt 47 lt gt 53 lt gt 58 lt gt 5 lt gt 71 lt gt 78 lt gt 86 lt gt 94 lt gt 103 lt gt 113 lt gt 121 lt gt 130 lt gt 139 lt gt 149 lt gt 160 lt gt 171 lt gt 184 lt gt 19 6 lt gt 207 lt gt 217 lt gt 225 lt gt 231 lt gt 237 lt gt 242 lt gt 245
69. luting process The parts of the fluidics are rinsed with diluent MIX and RBC chambers in HUMACOUNT PLUS and the measuring chamber in HUMACOUNT are filled up with 1 ml of diluent This method prevents the chamber s from dirt and makes the diluting process faster The sampling process has aspirated 25 ul of sample which is in the aspirating tip In the first diluting step the sample is dispensed into the MIX chamber in HUMACOUNT PLUS and into the measuring chamber in HUMACOUNT with 3 0 ml of diluent which comes from the Macro syringe through V13 On and Micro syringe while the M3 Dilutor motor moves upwards This process makes the 1 160 first dilution in the chamber e n HUMACOUNT PLUS the second sampling process aspirates 25 ul of primary dilution and then it is added with 5 0 ml of diluent into the RBC chamber This process makes the 1 32 000 dilution in the RBC chamber Sample diluent V11 DilWash V13 DilNeedle 2 FI dX V12 DilFloat l DILUENT kop LyseWbc A vg Cleaner x NL TY Xp V8 AspWbc Yr oatup CLEANER 7 Pressure Puffer Reservoir V1 DrainMix V V5 Puffer V6 DrainWash X lt Iv _ Waste Full Sensor TOL s EE ad RE WASTE In HUMACOUNT the same dilution rates are made but the second sampling the 25 ul of primary dilution is stored in the aspirating tip during the WBC measurement and the cleaning process and the
70. maiore M i e 1 E nrang mn f ri I u m E TITTT un i 2 i PRESSURE amp LIGIUD J SEN SOR ASARR PISTTAL HER BOARD HUMACOUNT Cabling Diagram 1 Per RERRB im nn PA VALVE BOARD ee LED BOARD EE aa rn Eu ee ETE ud ur MO and dicun LLLA Aah Hu rms odo 40 oH HER 4 DD SE I HEY AST eR SELO ithe ttre GE errr trot Pe ete err ELETTETTT RED GREEN MP LTTEETTET Ea z A Pree trees VALVE Wi Pe ek BE a ur aq z gt Hi T ex PORRO Mix PAANAN TO MOTOR X MI c i ami DILUTOR MOTOR OPTIO a NR m R WR RC NER E npn III P mE P Pe s FP ES 4 TAO sr uD r a Breite rnm PE A ARAMA rr Arr AE TEETE E 1 5IGMAL 5HIELO etter rt TEE EN II errr tet rrr iret et eet eos ott Pe RUE ER ere Teter tre
71. me opto board and the float opto stop position within a switches float float house specified time 3 Check the fittings and the tubes around the volume opto board The measuring vacuum has been dramatically Leakage in pneumatic system check Vacuum error decreasing during the the pump fittings tubings pressure measurement or sensor puffer reservoir and valves absolutely lost Motor error One of the Check the proper operation of the motor s Fatal stepper motors could modules home end opto switches ribbon error 905 not reach a specified cables and the motor moving CMD u position either it has MDilu dilutor MVert vertical Power off the system reached one of the MHori horizontal motor Example end switches or it St step error Ra range error Ra error reached one without Possibly error due to lost steps of the expecting to do so corresponding stepper motor Cleaner reagent container became empty or the dil clean Check Cleaner S OG If this error still remains with a filled cleaner container sensitive or there are reagent container adjust the sensitivity of lot of bubbles in the the dil cleaner sensor and check the cleaner tubes tubings and the fittings pe ose CTN Fatal MPNIF error Replace the MPNIF board Power off the system Fatal HGB error HGB channel did not Replace the MPNIF board Power off the system give a ready signal C
72. menu to check and or adjust the offset voltage of the amplifier Adjust the potentiometer to reach 0 mV Needle setting This submenu provides an easy way to set the needle position see Section 4 1 2 You can Log in as supervisor if the Multi user mode was selected in the User s Settings submenu Number 6 if present shows the availability of this function In supervisor mode you have the ability to change any user passwords and you have full access over user settings Always log out after this supervisor log in 5 2 12 Rescue Code for Multi user Mode It the supervisor password has been forgotten there is a rescue code for service purposes to access the supervisor level Rescue data are User ID 0 zero Name Service Password 729456 This forms a capital A on the keypad Service Manual HUMACOUNT amp HUMACOUNT PLUS 43 77 6 SERVICE OPERATIONS 6 1 Opening of the instrument The left side of both instruments is a side door which allows to reach the fluidic system easily Other parts of the analyzers can be serviced by opening the cover HUMACOUNT PLUS first unscrew the cover fixing Z screws located on the rear panel After this open the left side door In the front part of the analyzer there is a special zip fastener as it is shown in the figure Push this fastener to the direction of the arrow this will unlock the fastener and after that the cover is ready to open Pull the cover upward to op
73. metrical and the float may block the tube The long small angle chamfer must face to the bottom of the tube 2 2 5 Peristaltic Pump The pump generates the regulated vacuum and drains the fluidic system The pump is connected to the Valve 11 15 driver board MO connection and the control chip located on the MPNIF card which drives this unit The pump is operated from 12Vdc If the tube of the peristaltic pump becomes worn it can be broken causing Pressure error It is recommended to check the state of the tube and replace it every 2 years or after 20 000 measurements Always replace the peristaltic pump tube to the same PharMed type with the same length For servicing the tube of the pump open the peristaltic pump from its top see picture and remove the tube together with the white plastic side wall see picture HL 1 5 JU OL OSI JHL ALTER 4 sl 383H dl IHE aS ATS LIA lt NOWINS In case of damaged tubes it can be replaced by a new one by opening the two metal locks located at the two ends of the tube see picture 20 77 Service Manual HJMACOUNT amp HUMACOUNT PLUS Human 2 2 6 Chamber s In the HUMACOUNT PLUS there are three different chambers made of polycarbon The MIX chamber is for the first sample dilution The RBC and the WBC chambers are mounted with the
74. mplifier signal cable must be reversed analog signal ribbon cable it picks up noise Check the ribbon cable between the MPNIF board and the amplifier Maybe it is pinched under some screws or components This may cause trouble and even noise D Pneumatic failures liquid paths that conduct noise into the chamber liquid remains under the chamber in drain tube during measurement the conducting liquid remains inside the drain tube making noise to appear there Check chamber draining path for clogging or salt crystals Check the pump operation Since draining of the chamber goes under pressure control maybe bad pressure sensor or connection can cause trouble Clean the draining path Do not use alcohol but bleach Replace chamber if necessary liquid remains in the washing inlet at top of the chamber during measurement the conducting liquid remains inside the chamber wash tube making noise to appear The software is not compatible with the mechanics or related valve is bad partly clogged or the tubing is clogged loose lyse path guides in noise during counting if the a liquid in the draining tube is touching lyse reagent in T fitting noise can appear Check the lyse path and the lyse valve as well 54 77 Service Manual HUMACOUNT 8 HUMACOUNT PLUS Human 7 MAINTENANCE 7 1 7 2 7 2 1 7 2 2 7 2 3 7 2 4 7 2 5 7 2 6 7 2 7 7 2 8 Weekly Maintenance by User User should carry out on the fi
75. n calendar All dates will be converted accordingly LCD light off delay The time that elapses without any user activity to turn off high voltage You can select between the Gregorian and the Jalaali Persian calendar All dates will be converted accordingly 5 2 7 Service Testing Menu Service Testing menu provides tools for checking operation fluidics and hardware Testing From this submenu Self test can be run see Section 5 1 1 Self test 2 Valve test 3 Display test 4 Stress 5 Cleaning 5 cycles 5 2 8 Valve Test Menu In Valve Test menu valve numbers and their state are displayed The number under each valve represents the actual state of the valve Selected valve number is shown in inverse valve test 0 is Off state 1 is On state Valve 16 means peristaltic pump E 4 as me arre ao 1811 12 13 14 15 18 ODE HUMACOUNT has no valves 6 10 Select valve lt LEFT gt lt RIGHT gt Set valve lt UP gt off DOWN Toggle valves lt gt 995 5 mbar Use cursor keys for selecting and setting valve state or press OK to toggle state 42 77 Service Manual HUMACOUNT 8 HUMACOUNT PLUS Human 5 2 9 Display and Keyboard Test Display and keyboard test is provided to check keypad and LCD panel Press a key to test and the LCD will show the code of the pressed key and will be invert the colors at each touch of a key Last pressed keu for testing that every LCD dot is
76. nd the liquid detector Dil And Cleaner detector the Macro syringe doses diluent and cleaner reagents from the tubes through V13 Off V12 On V15 Off and V3 On into the chamber V11 DilWash V13 DilNeedle pag viapichomber V12 DilChamber DILUENT H CLEANER lt gt 14 ren V5 Cleaner Micro P Pressure Meter LYSE Puffer Reservoir P y w 24 V4 DrainPuffer 34 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 4 ADJUSTMENT There are some very important mechanical and hardware settings in both HUMACOUNT PLUS and HUMACOUNT systems and there are some software settings which are available only for Service Personnel The mechanical and hardware adjustments are described in this section Special software settings are included in the 5 2 section of this Service Manual 4 1 Mechanical settings There are two very important and necessary mechanical settings in both instruments e Opto wheel setting e Aspirating tip setting All analyzers are adjusted by the manufacturer before shipment but there is a possibility to change any part of the mechanical system and in this case the Service Personnel must do these important settings The omission of these settings can cause malfunction or damags to the instrument 4 1 1 Opto wheel setting This setting may be necessary for the vertical motor movements because this adjustment sets the opto d
77. nic Block This block contains the main electronic parts of HUMACOUNT PLUS the cables and the main board holder Valve Block 111 Pressure and Reagent Sensor Board Interface connectors plate MPNIF Card IDEPROM Board N 3 2 FI disk dri Service Manual HUMACOUNT 8 HUMACOUNT PLUS 23 77 HUMACOUNT PLUS Mechanic and Fluidic Block This block includes the mechanic and fluidic parts see 2 2 of HUMACOUNT PLUS and it is located on the assembly plate The analyzer has a door with magnetic lock on the right side which makes easy to access the mechanic and fluidic system Sampling needle H amp V Moving Unit Reagent Sensors Puffer Reservoir Valve Block Ill 42 27 Block 24 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 3 3 HUMACOUNT Electronic Block This block contains the main electronic parts of HUMACOUNT the cables and the main board holder on a rack system plate mM T EN pote BI ow Zu Main Board sm MPNIF Card m M Floppy disk drive Power Switch Speaker IDEPROM Board Service Manual HUMACOUNT amp HUMACOUNT PLUS 25 77 2 3 4 HUMACOUNT Mechanical and Fluidic Block This block includes the mechanic and fluidic parts see 2 2 of HUMACOUNT and it is located on the assembly plate The analyzer has a door with magnetic lock on the right side which makes
78. nimal length of 40 cm Please check the length of this tube because if a short tube is installed only and the cleaner is contaminated the PLT blank could not go down due to the diluent mixing with contaminated cleaner This reason can be sorted out easily replace the cleaner reagent with diluent and PLT blank should go down How can a good reagent become bad by time f the reagent tube was contaminated and some bacteria begin to grow inside once you put an infected reagent tube into a new tank by time it can become infected as well i e the background PLT blank becomes high Wash the reagent tube which is in connection with the reagent with 1 of bleach solution then rinse with clean distilled water or diluent It can avoid the bacteria to grow inside f tank is open and is not installed or closed external dust can make reagent dirty 6 8 2 Bad earth grounding In this case external ground referenced noise can get into the system by ground coupling If system ground is not good enough ground terminal can become a noise source as well i e external signals will be coupled into the system instead of protecting it If no earth ground is available you can use the 4 mm diameter screw at the rear panel to connect a ground potential to the case so that noise immunity can be increased Measure voltage on ground terminal to make sure earth grounding is correct AC voltage lower than 1V is accepted in this case A
79. nnection to the amplifier Light sensor Service Manual HUMACOUNT amp HUMACOUNT PLUS TITT HUMACOUNT PLUS makes four kinds of HGB measurements diluent measurements are performed on diluent reagent e Sample dark measurement before WBC counting e Sample light measurement before RBC counting e Diluent light measurement in WBC washing phase e Diluent dark measurement immediately after the previous one The HGB result is calculated from these measurements by HGB log CNT aiuentlight CN Tailuentdark CNT samplelight CNT sampiedark HUMACOUNT does not perform any dark measurements it is always zero so this equation is simpler HGB log CNT ciruentight CNT sampielight Do not open or close the instrument door during any measurement cycle because this may cause error in HGB measurement Normally door should be closed during real measurements 2 1 3 Volume Opto Board This board is present only in HUMACOUNT PLUS It contains three opto switches which make possible to measure a constant volume of the diluted sample with a special float The volume opto board is connected to the amplifier board through the VM connector on the other side of this board Measurement is made between OM1 and OM3 no LED on OM2 gate opto is for bubble error lt OM1 Top opto prevention The upper LED is lit when the float is between the OM1 sensor The lower LED is lit when the float is between the 4
80. nnector Power Switch Connector ISA slot for MPNIF card MBA board contains the necessary connectors for the different resources and an ISA slot for the MPNIF board There is a 3V Li battery to feed Real Time Clock and Calendar RTCC chip during power down Floppy disk drive is connected to the MB4 with a ribbon cable The required configuration of the floppy disk drive boot sequence can be set in the Boot priority section of the BIOS settings Video adapter MDA Monochrome Display Adapter and video memory are placed on the MPNIF card In MDA mode the instrument displays the text monitor output on the LCD Two LEDs are indicating the state of power on MB4 main board 1 Green LED is on if external power is present stand by mode 2 Yellow LED is on if power is turned on Always remove external power or turn off power switch on the power supply if you remove or replace components e g DimmPC MPNIF MB4 main board etc 2 1 15 DimmPC Module The 4 board incorporates a credit card sized PC named DimmPC The processor on the DimmPC is a 133MHz Pentium class core with 32Mbytes on board RAM and 32Mbytes on board SanDisk This is the HDD hard disk drive of the analyzer so instrument software with all user settings calibration database etc is stored on the DimmPC DimmPC is the Trade Mark of Kontron Embedded Modules GmbH Service Manual HUMACOUNT amp HUMACOUNT PLUS 15 77 NU Hard Disk Flash SanDisk
81. nputstree _ You cn power fr ne sytem 5005 Apertures are partly cogged soos Please emp waste contained 10 oeu aata vill be copie to tne databas _ CI 2 Insert data Insert data disk d of jou data record s will be saved on d disk s Insert an empty floppy disk Insert next empty floppy disk 5106 No such type of data Your previous QC data will be lost Are you sure Some or all of the calibration factors are out of range 0 or all of the calibration factors are out of Some or all of the calibration factors out of range 0 This function is not available because there are no accepted blank values ka a Insert software Jp disk 5902 Are you sure to delete all device statistics O Are you sure to delete all device Are you sure to delete all device statistics O This function will delete all measured results AY 5905 Are you sure oo This function will delete all selected records Are you sure Are you Are you sure 00 Service Manual HUMACOUNT amp HUMACOUNT PLUS 51 77 6 7 Problems related to Warning flags In this section we give you examples and troubleshooting of common problems WBC blank measurement error C B warning flags displayed but the WBC histogram is correct no noise There is no backlight in the LCD Module All measured parameters are 0 but HGB result is correct Analyzer always e
82. o the HVB because the over voltage on the output of the HVB can damage the HVB board and the amplifier Service Manual HUMACOUNT amp HUMACOUNT PLUS 11 77 2 1 8 Graphic LCD Module This module contains 240x128 dots LCD display HITACHI LMG7400PLFC or 5 14 002 the HVB and this is the back panel of the keyboard module LCD has a high voltage backlight lamp the high voltage board generates the required voltage LCD Connection to MPNIF Connection to backlight lamp Connection to amplifier High voltage board IU Tum II 2 a Fern 4 L B Li e E year i LAN 1 MIN a m 3 5 n axo My n LS ur a Temperature sensor Keyboard connection Ground connection LCD assembly of HUMACOUNT PLUS Inthe HUMACOUNT the HVB is fixed onto the LCD display holding frame Connection to LCD backlight lamp HVB Old version In the LCD module and on the MPNIF card there is a special temperature compensation circuit which makes possible to use the LCD module in wide temperature ranges with the adjusted contrast 12 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 1 9 Keyboard Panel The analyzer has a 24 foil keyboard including numerical keypad 0 9 cursor moving and OK buttons Delete and Help keys and 6 function buttons under the LC
83. oli indexing file Fatal system or EE REPE oS program error Fatal pressure error Cannot make the Check the pump and the tubes fittings and Power off the system measuring vacuum valves around the puffer reservoir Waste is full ar Empty waste container Empty waste container If it is not full there Waste container is a clogging in the waste sensor line The system assumes that is full maybe Valve15 is not closed completely you will do it going or tube is pinched on 2 Replace the Diluent reagent container with fill empty or the diluent Min Check Diluent container fluid sensor is too this error still remains with a filled diluent sensitive or there reagent container adjust the sensitivity of the diluent sensor and check the tubings are a lot of bubbles an in the diluent tubes and the fittings Es nn Replace the Lyse reagent container with a fill empty or the lyse Check Lyse container fluid sensor is too If this error still remains with a filled lyse sensitive or there reagent container adjust the sensitivity of are lotorbubblasin the lyse sensor and check the tubings and the Iyse tubes the fittings 48 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human Enora Ress remy 1 Try cleaning to remove clog 2 Check the proper operation of the The float in the volume opto board did not Timeout Try cleaning reach the start or the volu
84. on rate and good mixing with Iyse the syringe also pushes some air bubbles for mixing V11 DilWash EB Eum ei Diluent amp Cleaner V5 Cleaner m Sensor 4 V15 Ed ER V3 Bubble 13 DilNeedle 1 Lyse Sensor Dil 3 P Pressure Meter LYSE Puffer Reservoir V1 DrainChamber v2 Ly 3 ur __ DrainPuffer Service Manual HUMACOUNT amp HUMACOUNT PLUS 31 77 3 6 WBC counting process In HUMACOUNT PLUS at the beginning of the WBC counting process the analyzer moves the float up by diluent reagent coming from the macro syringe V12 amp V7 are On V10 V9 and V3 are OFF and pump drains the reagent By this time the pump has generated the regulated vacuum in the puffer reservoir After that the vacuum aspirates the diluted sample from the WBC and RBC chambers through V3 ON and V5 ON valves while all others are OFF during this the pump is OFF which is equivalent to a closed valve Both WBC and RBC samples are flowing through the aperture but the float measures only the WBC sample volume by moving down until the lower opto switch is off When the float reaches the lower opto V3 is closed In this state the HGB measurement is executed V11 DilWash V13 DilNeedle 2 V12 DilFloat puent V NG Ne vi 0 AR V7 FloatUp V3 en 8 AspWbc 1 1 Micro Pressure
85. r mode can be disabled on the user level Disable 3 part diff Disable 3 part diff for using quick lyse without 3 part Disable markers differential the errors and the bad 3 part parameters can be Stretch histograms excluded from the results Language English Native keyboard Maintenance day Thursday ers Patient data Birthdate application Stretch histogram setting this option to Yes will modify the WBC histogram from 0 300 fl instead of 0 400 fl this is Disable markers setting this option to Yes will omit the markers from the reports it is commonly used for Veterinary recommended for Veterinary mode Language this setting will adjust the user language The Service menu always in Available languages Arabic English French German Greek Hungarian Indonesian Italian Polish Portuguese Romanian Russian Spanish Turkish and Vietnamese Chinese language is available only for special models Native keyboard Setting it to Yes will utilize the external PC keyboard with the language specific layout Maintenance day You can select among the days of the week According to this setting the instrument will warn the user to do the weekly maintenance when shut down Service Manual HUMACOUNT amp HUMACOUNT PLUS 41 77 Patient data There are two options Birthdate and Age According to this setting in the sample information dialog and in the database the instrument will prompt for the age or the date of birth If age is sp
86. rate the system without grounding 2 This error can happen also if some other instruments X ray monitor are working near the instrument by making strong radiation or from the same electrical outlet by generating fluctuation in the line which causes noise 3 Some extreme conditions other reagent systems this problem can happen with good grounding as well In that case the blood samples make a thin fluidic film in the WBC draining tube lipids lysing reagent This fluidic film conducts the noise to the WBC measuring electrode To prevent the system from this problem we recommend to use the built in Autoclean procedure which cleans up the fluidic film from the WBC draining tube with the cleaner reagent after every Xth measurement The default value for the Autoclean cycles is 20 and it can be changed only from the Service menu gt Replace the WBC measuring tube aperture Check the WBC chamber and replace it if it is damaged Check the 34 pin and the 20 pin ribbon cables between the MPNIF Amplifier High Voltage boards Reconnect if you find something loose Replace the High Voltage Board HVB Reconnect the shielded cable at both ends MPNIF and Amplifier Move the volume meter tube to the lowest position and check the orientation of the float within the tube Measure grounding quality with a voltage meter by measuring AC voltage between grounding and neutral This voltage must be less
87. rors No CPU Speed 133 MHz ISA bus cycle duration 800 ns Device Configuration submenu Local Bus IDE Adapter Enabled Floppy disk controller Enabled Base address Primary Serial port A 3F8h IRQ4 Serial port B 2F8h IRQ3 Parallel Port Enabled EPP 378h 46 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human Setup Boot Menu Floppy Check Disabled Summary Screen Disabled QuickBoot Mode Enabled Dark Boot Disabled Boot Device Priority submenu Hard Drive Add on cards SanDisk Primary Slave Removable Devices Legacy floppy drives CD ROM Network Boot 6 4 2 Exit Menu Exit Saving Changes Saves all the selections and exits setup The next time you boot the BIOS configures the system according to the Setup selection stored in CMOS Exit Discarding Changes Use this option to exit Setup without storing in CMOS any new selections you may have made The selections previously in effect remain in effect Load Setup Defaults Select to display the default values for all the Setup menus Discard Changes If during a Setup session you change your mind about changes you have made and have not yet saved the values to CMOS you can restore the values you previously saved to CMOS Save Changes Saves all the selection without exiting Setup You can return to the other menus if you want to review and change your selection Would you make a mistake or get lost among settings you can restore defaults anytime
88. rst workday before starting up the analyzer Cleaning the washing head User should clean the lower surface of the aspirating tip washing head using a soft cloth immersed in warm tap water to remove salt build up Periodic Maintenance by Service The instruments should be checked and maintenance must be carried out in every 6 12 months or after 10 000 measurement cycles Check Self test and Device statistics Run the built in Self test and check the overall test result Check the device statistics to find common problems Cleaning and Greasing Dilutor Block The dilutor block driving wheels and gear bar should be cleaned from dirt and must be greased between the gear bar and the support and between cogged wheels Checking and Lubricating Dilutor Piston Tips The cogged end of PTFE dilutor pistons should be cleaned and lubricated by neutral silicon grease Apply just a thin layer and move it along the perimeter of the piston so that some of the material goes into the gaps between the sealing rings Repeat this step for Iyse and dilutor pistons as well Check the condition of the micro piston sealing and replace if necessary Cleaning and Lubricating Needle Moving Mechanics The H amp V moving mechanics sliding bars should be cleaned from dust Lubricating of the sliding bars must be made using Photorube oil containing PTFE Grease or pure lubricating oil is not suitable Checking and Replacing Washing Head Check the state of t
89. stogram SNO lt HT gt 152 lt LF gt internal identifier DATE lt HT gt 19980715 lt LF gt date of measurement YYYYMMDD TIME lt HT gt 114500 lt LF gt time of measurement HHMMSS SID lt HT gt 1AXX435 lt LF gt sample identifier alphanumeric PID lt HT gt B 2D44 lt LF gt patient identifier alphanumeric CHN lt HT gt 256 lt LF gt number of histogram channels 256 9 lt HT gt value of first histogram channel 1 lt HT gt value of 255 histogram channel value of the last histogram channel Service Manual HUMACOUNT amp HUMACOUNT PLUS 65 77 9 2 7 Transmission Example Let us suppose that the receiver was not ready to receive when HUMACOUNT PLUS HUMACOUNT was switched on therefore the instrument is not starting a communication Receiver sends that it is ready to receive now HUMACOUNT PLUS HUMACOUNT sends acknowledge lt ENQ gt lt ACK gt HUMACOUNT PLUS HUMACOUNT INIT next time to initiate link there is data to send V1 7 software lt SOH gt AI lt STX gt HUMAN HUMACOUNT PLUS lt HT gt 1 7g lt HT gt 20010405 lt HT gt 163902 lt E T X gt 88 lt EOT gt V2 x software lt SOH gt AI lt STX gt HUMACOUNT PLUS lt HT gt 2 15a lt HT gt 20010405 lt HT gt 160734 lt ET X gt E4 lt EOT gt Receiver sends acknowledge lt ACK gt lt SPACE gt A HUMACOUNT PLUS HUMACOUNT sends DATA package lt SOH gt BD lt STX gt SNO lt HT gt 1 lt LF gt DATE lt HT gt 20010405 lt LF gt TIME lt HT gt 163800 lt LF gt SID lt HT gt 1 lt LF gt PID lt HT gt lt
90. suuaqunanasa m CATHODE COLD LAMP CONNECTOR MAA AE PEE EE EASE HAM Ter OOO INT Horan Sa Ca nmm k EEE PT ELIT AMIN m Imma NN Perret tt tree Pere er ssa Na 3 amp i EE LTO ARCUS CABLING DIAGRAM ARCABL10 5CH Service Manual HUMACOUNT amp HUMACOUNT PLUS 71 77 ai ET H2S ZT U3H2d i A cuir h Qh F 39ULIOA HJIH snowed Qi HONIUIO TETTE HUMACOUNT PLUS Amplifier High voltage amp HGB Boards 9 8 H31H3AMI 5 SIHI T nzb MOOT I xT HOST L Ir Stuf parama MOT m oorssah Ul T Bz2 572 XT HEE hie AB o LZ szu IL m HOST BeTeNT BTH AB 50 Z8T98 HSH J TTM TH EE LEdo T OTSOOTEL3H OT 501223H ADSE NDE 1 1 Hoy SEI 25 eri za XT wor 5 Ha 47 k XE i em wo ZEX ors orsi arate MOST HOST HOST Bu Pe 82 zu L
91. t SOH gt MID CMD lt STX gt MESSAGE lt ETX gt CHKSUM lt EOT gt MID Message ID one capital letter between and 2 CMD Command one capital letter package type descriptor for INIT D for DATA R for 9 W for WBC e P for PLT MESSAGE the message consists of ASCII characters between 32 128 CHKSUM two hexadecimal digits of a one byte checksum which contains the lower byte of the sum of characters between the lt SOH gt and lt ETX gt incl Those too 9 2 3 Format of Acknowledge of the Receiver The receiver must acknowledge transmission by the following message lt ACK gt CMD MID CMD the type of the next package to send this makes possible to a request for histograms If no more packages are required SPACE should be sent MID the identifier of the package that acknowledged by this message If receiving of the message was not successful lt NAK gt should be sent and HUMACOUNT PLUS HUMACOUNT will repeat the last package The receiver has approx 1 second to reply otherwise HUMACOUNT PLUS HUMACOUNT will repeat the last transmission twice automatically But if still no response after 3 trials HUMACOUNT PLUS HUMACOUNT will not start to communicate any more even if there is data to send see General Description 9 2 4 Detailed Description of Packages The packages sent by HUMACOUNT PLUS HUMACOUNT are placed between a header and a tail There are 3 main types of them INIT DATA and histogr
92. t in Service Miscellaneous menu The instrument will move the motors to setting position and hold the carriage so that adjustment can be made safely without unintentional moving of the carriage Set the point of the tip to the washing head s bottom plane while the carriage is held by motors Needle setting menu Fix the B screws After setting exit from the Needle setting menu and check the position of the tip and if it is necessary make the adjustment again 4 2 Hardware settings The most part of the hardware settings are adjusted by the manufacturer and it is not required to reset these settings There is only one part in both instruments which claims some adjustment this is the sensitivity of the liquid detectors 4 2 1 Adjustment of the liquid detector sensitivity Liquid reagent detector sensitivity may be necessary to be adjusted Follow the procedure below to find the optimal setting 1 Without any reagents in the sensor tube set the Diluent Cleaner and Lyse potentiometers in the PCPRO board to reach the state when the LED goes off 2 After that turn the potentiometers counterclockwise to reach the on state the LED is on and after that turn 180 more in the same direction 3 Check that LEDs go off when priming with reagents To check the proper operation of the liquid detectors use the indicator LEDs Reagent with bubbles LED sometimes turns on Reagent without bubbles LED is off 36 77 Service Manual
93. t some places as a bad practice electricians like to connect earth ground terminal to neutral wire Depending on the resistance of the neutral back wire where it is really earthed several volts can appear and this way any inductive noise will be coupled into the instrument It is better to create a real earth grounding and connecting it to the rear screw 6 8 3 External electrical noise If another instrument near HUMACOUNT can radiate electromagnetic signals in the 1 kHz 100 kHz frequency region it can be picked up by the system especially if they are very close to each other or the grounding is not quite perfect You can easily identify this noise source by relocating the instrument noise high PLT blank disappears In this case you have to identify the possible noise source switch mode power supplies computer monitors since they are not shielded centrifuges due to high switching noise of rotor contacts etc the power of the electromagnetic source because if high power is present maybe relocation does not solve your problems sometimes the electric power supply makes the coupling so UPS solves the problem Another source of coupling in external noise can be the reagent tanks and tubes Especially radio transmitters can cause problems of radiating so that even the reagents diluent guides in the noise A metal pack for the diluent tank then a good earth grounding of this metal box allows the coupling to disappear forever S
94. the instrument makes a blank measurement if it was in stanby mode default 1 hour If it is set to O then no ReBlank is performed Rinse time If this time is set then the instrument will make an automatic extended washing procedure with more diluent of the chamber s to get wash dirt out of the chambers dust If it is set to O then no Rinse is performed Serial speed instrument can send results to a host computer via serial port with the RS232 V24 protocol The baud rate of this serial communication must be selected at this point For details of the serial communication protocol see Appendix 9 2 in this manual This setting must be set to the desired baud rate otherwise the link to PC is not possible Serial Protocol Version with this option the used protocol sending specific fields or not can be selected This is useful when a new software with more stored e g patient info is installed in laboratory system where an earlier serial protocol has been implemented Waste container capacity HUMACOUNT only to set the correct volume of the waste container for proper usage of the software waste full alert Set this value 2litres less than the total volume of waste container Laboratory Header Lines this setting controls the available lines to set up the laboratory header on printed reports The header text can be edited on user level Settings Disable multi user mode by this setting the multi user mode Disable multiuse
95. the selected probe with HSW signal The DHON signal from the MPNIF card switches on the LED in the HGB head via a transistor Q3 but the Photo Detector in the HGB head is working continuously in m Connection to the Reed relays Connection to the MPNIF high voltage board card AMP and DIGIO HUMACOUNT amplifier board includes only one input connector for the chamber In this board there is no relay in the input stage but there are two opto switches U1 U3 TLP627 to connect high voltage from high voltage board to the probe with HSW signal and isolate the input of the amplifier The RSW signal changes the gain RBC WBC in the feed back of the second amplifier stage with U2 MAX319 analog switch The DHON signal from the MPNIF card switches on the LED and also the Photo Detector in the HGB head via e is no dark HGB value in HUMACOUNT a transistor Q3 that is why ther fen L AB EC BH T I 4 7 LIN 1 L Fr aiba t m aac kush el r Connection to the MPNIF card Connection to the high voltage board w GH 4 FIT 24 I VEA Cy 3n U The other side of the amplifier boards includes special connectors for the chamber s and connector for the HGB head 4 Service Manual HUMACOUNT amp HUMACOUNT PLUS 9 77 2 1 5
96. the software allowing to change the FPGA functions If there is any problem e g power off during this overwriting process the instrument will start in Safe mode this is displayed on the LCD and the FPGA will be configured from the Safe E PROM IDEPROM 2 1 7 High Voltage Board HVB High Voltage Board generates the backlight voltage 300Vrms 180 kHz for CCFL cold cathode fluorescent lamp of the LCD the aperture cleaning voltage 150Vdc and the measuring voltage 50Vdc The high voltage board is connected to the system through the amplifier board and the MPNIF card This unit contains INVC191 inverter which is a high voltage high frequency circuit producing suitable voltage for CCFL of the LCD Connection to amplifier Connection EU rn E to LCD lamp E i gt her O O EM NUN E R5 FT i P una kal ILLI D ees Y 7 k LU LEE The CFSW digital signal from the MPNIF controls the high voltage board if it is high 1 the inverter is off if it is low 0 the inverter is on The MVON digital signal from the MPNIF card switches the measuring voltage 50 Vdc on off by an opto switch Warning Be careful with servicing this board in active state because the high voltage 300V at LCD lamp connector can cause damages or electric shock Never operate the analyzer without the LCD backlight connected t
97. vicing the instruments perfectly and avoid extra costs and wasting precious time This HUMACOUNT PLUS HUMACOUNT Service Manual contains the functional descriptions of both analyzers the operation of the fluidic systems the adjustments and settings and very important information for the Service Personnel about the service operations and possible problems 11 Name and serial number Name HUMACOUNT PLUS HUMACOUNT Hematology Analyzer Serial No Every instrument has its own serial number which is printed on the rear panel label and it can be read out from Device Information or from the Self test submenu This serial number is write protected by HUMAN 1 2 Intended use HUMACOUNT PLUS HUMACOUNT hematology analyzers are fully automated cell counters for in vitro diagnostic use The compact instruments were developed for small to medium size labs HUMACOUNT PLUS can process 60 HUMACOUNT can process 30 samples per hour and they are intended to determine the following 18 hematology parameters from a 25 ul whole blood sample WBC GRA LYM MID GRAY three part WBC differential e HGB RBC HCT MCV RDW MCH MCHC e PLT MPV PCT PDW 1 3 Integrated software The integrated software controls the instrument operations displays stores recalls data and allows the User to perform and calibration procedures and modify the user settings The software version number can be read out from the Device In
98. xits from the measurement with Timeout error WBC noise during measurements blank and normal Noise in the WBC histogram S warning flag The instrument measures the probe voltages for both apertures during the measurements From these values and from the measuring times the analyzer can detect the clogging C c warning flags If the WBC probe voltage is changed the instrument will give a C warning flag and the WBC value will have an E error flag This fluctuation of the probe voltage can happen if the diluent reagent temperature is changed or there is a passage of the measuring current next to the aperture It can be caused by bad aperture or the WBC chamber is damaged If the LCD is blank and the Self test shows electrode voltage and current is low there is no high voltage in the system The problem is that the inverter circuit that generates this high voltage is out of order or there is no connection between the MPU Amplifier High Voltage boards The high voltage board may be damaged Shielded cable that connects MPNIF board to the Amplifier board is disconnected In that case only the measured HGB value is available because it comes through the ribbon cable The volume meter tube is not in lowest point 1 If there is not a good grounding in the system the instrument can give such kind of errors HUMACOUNT PLUS HUMACOUNT has a shock protecting system that requires earth grounding You must not ope
99. ystem and the connection between the motor and the DM ribbon cable from the MPNIF card On the other side of the board there are two opto switches home and end switches and their own indicator LEDs 2 1 13 Valve Driver Boards In the HUMACOUNT PLUS there are three kinds of different valve driver boards e Valve board 1 5 e Valve board 6 10 e Valve board 11 15 amp pump In the HUMACOUNT there are only two kinds of valve driver boards e Valve board 1 5 e Valve board 11 15 8 pump 4 The Valve 11 15 driver board contains the MO connection to the Pump unit ss N viz E The valve driver boards are connected to controller chips located on the MPNIF card through A B and C ribbon cables In HUMACOUNT there is no B cable These boards control the valves in 5 valve sets 14 77 Service Manual HUMACOUNT amp HUMACOUNT PLUS Human 2 1 14 Main Board MB4 The analyzer works with a main board called MB4 Required settings of MB4 board and BIOS setup values are detailed in Chapter 6 Connector ATX Power Supply Connector Keyboard Bi Connector DONC rw 2n us y T E N pu E kabon x 1 MADR yt bo endet AU ipm Standby Li battery ae LEDs Printer Connector Speaker lt N Co

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