Fluoview FV1000 safety guide
Contents
1. CHS1 G 1 FITC HV Gain Offset mf 2p sd BS sl e SS RER PEN ET ER v Laser 48g 96 0 ionCor DRE S O APA E Focus x2 VA Focus X4 xY Repeat ips Er CHS1 6 1 7 CHS2 6 2 g c os g su TD FITC on cee ea HV Gain Offset HV Gain Offset HV Gain Offset HV Gain Offset CA Lamp L2 4 2J 41 2 2 a 2 EJ EE x ze d oum 31 Shows the laser beam being output Lambda Focus x2 i Depth Twin 2 Focus x4 XY Repeat xy Stop Time Bleach Stop iv CHS1 G1 Y CHS2 G2 MEN os Il m1 SU TD FITC cys _ IA HV Gain Offset HV Gain Offset HV Gain Offset HV Gain Offset z NM Ga ae ralla sa za X ye acc crue La pa o um 31 al auto mm SAFETY GUIDE 1 2 Page SAFETY CAUTION Objective Lens Beam Angle Offset UAPO 150X OTIRFM NA 1 45 vj 0 4 Default Mirror Unit FS IBEVA 11 0mm 4 Excitation DM Beam Collimator Adjust DM488 543 633 Laser and Trigger control Refractive index of sample 1 33 E Laser Control Penetration Depth v 488 ql 0 nm In case of Manual mode Laser beam being output appears In case of Trigger Input or Key Assign mode y i M Spenser COT anon Laser beam being output or Laser emission being waited appears When Trigger Input mode is used and even if Laser emission button is set to the state o2. is heated quickly moisture may condense on the equipment and could irreparably damage the optics e In a place subject to direct sunlight e Outdoors e Inaplace where a flammable gas is produced In a place with excessive vibrations e Onanunstable surface of floor or platform Please carry out about exchange of consumable materials according to the handling description of each unit 3 4 Recommendations for Use e Combination with either an upright microscope or an inverted microscope use ordinary biological objectives Therefore the observed specimen should be prepared using a cover glass with thickness of 0 17 mm or a petri dish with bottom thickness of 0 17 mm The use of a special cover glass is recommended for observations using immersion objective for LSM e When using this system we recommend turning the indoor lighting low in order to reduce external disturbance light on observations The indoor lighting particularly affects the differential interference observation images SAFETY GUIDE eo Page 3 3 CAUTION FOR SYSTEM OPERATION Care and Storage e To extend the life of laser head while it is not used for a long period it is recommended to perform 8 hours of continuous aging at least o
3. laser Multiline Ar 22mW output linear polarization laser forced air cooled Ar ion laser 457 9nm 488nm 514 5nm FV5 LAMAR 2 Power consumption 90 264V 14A max Multiline Ar 40mW output linear polarization laser forced air cooled Ar ion laser 458nm 488nm 515nm FV5 LAMAR Power consumption 100 240V 20A max HeNe G laser 1 mW output linear polarization FV10 natural air cooled Green HeNe laser 543 5 nm LAHEG230 2 Power consumption 90 132V 0 65A max 180 264V 0 65A max HeNe G laser 1 mW output linear polarization FV10 natural air cooled Green HeNe laser 543 nm LAHEG230 Power consumption 115V cie 230V 0 2A max Semiconductor LD405 Head output 25mW liner polarization Laser With temperature control circuit using Peltier device LD440 Head output 25mW liner polarization With temperature control circuit using Peltier device LD473 Head output 15mW liner polarization With temperature control circuit using Peltier device LD559 Head output 15mW or more liner polarization With temperature control circuit using Peltier device Power consumption AC100 120 220 240V 0 3A max 0 15A max 50 60Hz LD635 Head output 20mW liner polarization With temperature control circuit using Peltier device SPECIFICATIONS Page 1 1 SPECIFICATIONS Specifications of Units Specifications Scan unit FV10 SU Basic The Scan Unit FV10 SU is adapted to the back of microscope using Construction Microsc
4. 0 15A max Supply Unit Multiline Ar Laser AC90 264V 14A max FV5 LAMAR 2 Power Supply Unit Use any of these items AC100 120V 220 240V Multiline Ar Laser 20A max 10A max FV5 LAMAR Power Supply Unit SPECIFICATIONS 1 8 Page PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided please select the proper power supply cord for the equipment by referring to Specifications and Certified Cord below CAUTION In case you use a non approved power supply cord for Olympus products Olympus can no longer warrant the electrical safety of the equipment Specifications Voltage Rating 125V AC for 100 120V AC area or 250V AC for 220 240V AC area Current Rating 6A minimum Temperature Rating 60 C minimum Length 3 05 m maximum Fittings Configuration Grounding type attachment plug cap Opposite terminates in molded on IEC configuration appliance coupling Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2 The fittings are to be marked with at least one of agencies listed in Table 1 In case you are unable to buy locally in your country the power supply cord which is approved by one of the agencies mentioned in Table 1 please use replacements approved by any other equivalent and authorized agencies in your country Country Agency Certification Country Cert
5. Labels CAUTION UV EMISSION HIGH SURFACE TEMPERATURE Do wol slate abo wllraniolel tars fo aol irradiate ullraviolel rars lo skin Bo mol sol fingers te the liah guide mounl f SAREE Eni EWE RT CRAY SARTERCATAVTC EAM 774 NAD RE EA NG CEA Other protective cabinet labels than those shown above are attached to other positions on the product These labels are for Olympus service personnel to refer to For replacement of soiled or peeling warning labels please contact your Olympus representative SAFETY GUIDE amp 8 AX CU EC h k Page 2 17 CAUTION FOR SYSTEM OPERATION Transportation of Equipment 3 CAUTION FOR SYSTEM OPERATION 3 1 Transportation of Equipment e This system has been assembled and set up by specialist engineers and technicians Avoid moving the system whenever possible because this could alter the alignment of the optics Please consult your local Olympus representative if you want to move or displace the system If the system is moved by the user Olympus cannot be liable even if system trouble or damage occurs 3 2 Treatment of System e The laser tube used with this system should always be disposed of in accordance with local industrial waste treatment regulations When disposal is req
6. OLYMPUS User s Manual Safety Guide FLUOVIEW FV1000 CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPE FV10 ASW Ver2 0 Thank you for your purchase of Olympus microscope at this time AX7807 Retain this manual in an easily accessible place near a system for future reference CAUTION A CAUTION FV1000 is a CLASS 3B laser product The procedures for using this system are classified as follows e Service Service means any adjustment or repair performed by highly trained and skilled technical personnels who are provided the service training following to the service manual for this system The performance has influence on the feature of this system and there is a risk which unintended CLASS 3B laser light is emitted e Maintenance Maintenance means adjustment or other procedures performed by customers to maintain that this system functions properly e Operation Operation means all performance described in the user s manuals in this system CLASS 3B laser light is only emitted from the objective lens during the actual execution The User s Manuals of this system consist of the following In order to maintain the full performance of this system and ensure your safety be sure to read these user s manuals and the operating instructions for the laser unit and light source unit before use e FV1000MPE FV1000 User s Manual Laser Safety Guide e FV1000 User s Manual Safety Guide e FV1000 User s Ma
7. SYSTEM OPERATION Handling Precautions e The air outlet of the laser cooling fan blows out warm air Do not place a flammable or weak object near the heat from the air outlet Also to allow smooth heat radiation provide clearance of at least 50 cm around the air outlet Otherwise overheating of the laser tube may destabilize or stop the laser oscillation The rise in the equipments internal temperature may also cause malfunction or failure e The laser power supply unit incorporates a cooling fan on the front and rear To avoid blocking the area surrounding the cooling fan or the slits at the top and sides of the power supply unit install the unit at a distance at least 30 cm from the surrounding walls Also do not place objects such as papers or books on the laser power supply unit for this may lead to overheating of the laser power supply unit or activation of the protection circuitry causing stoppage of the laser oscillation e The Power Supply Unit Laser Combiner and computer have a cooling fan on the rear To prevent malfunction be sure to reserve a clearance of at least 10 cm around their air outlets e Regarding Z direction move besides mechanical limitation of microscope a limit can separately be programmed in software within mechanical limitation If a software limit is set to the position where objective lens or specimen comes close each other for example the damage of specimen that occurs by a contact with objective lens ma
8. TIONS Specitications of Units Specifications Fluorescence Detector Unit Filter type Detector Hamamatsu Photomultiplier R7862 fluorescent 1 2inch R6357 high sensitivity type Detector 3ch standard 1ch option FV10 FD Single Pinhole Pinhole size Q50 800um changeable 5um step SDM HQ ion coating Barrier Filter 3CHs standard Selection 6 position turret for SDM Barrier Filter on each CH Dimensions 2 0 W x 162 D x 144 H mm Spectra type CH1 and CH2 has individual optical gratings and slits Detector The grating angle defines the wavelength of the detecting fluorescence The wavelength width are selected by the slit on each channel Spectral fluorescent detector FV10 SPD Spectra channel Number of Channels 2 Resolution 2nm Setting resolution 1nm owitching speed 1ms 100nm Wavelength Width 1 100nm Channel separation SDM 6 position Filter Number of Channel s 1 or 2 Channel s SDM HQ ion coating Barrier Filter 6 position each Detector Hamamatsu Photomultiplier R7862 1 2inch R6357 high sensitivity type 3ch standard 1ch option Pinhole Single Pinhole Pinhole size 50 300um changeable 5um step Selection SDM Grating type spectrometer CH1 CH2 SDM HQ ion coating Barrier Filter CH3 CH4 Dimensions 270 W x 162 D x 144 H mm Weight 6 6kg SPECIFICATIONS Page 1 3 SPECIFICATIONS Specifications of Units Power Supply Unit FV10 PSU Po
9. Y GUIDE 2 2 Page WARNING LABELS Laser Safety Warning Labels e Combination with BX61WI AVOID ps um RADIATION 15 EMIT ROM THIS APERTURE SAFETY GUIDE 4 LLL Page 2 3 WARNING LABELS Laser Safety Warning Labels e Combination with IX81 Objective lens 2 Filter holder 2 Condenser lens SAFETY GUIDE O 2 4 Page WARNING LABELS Laser Safety Warning Labels 2 1 3 Protective Housing Labels CAUTION CLASS 3B LASER RADIATION WHEN OPEN AYOID EXPOSURE TO THE BEAM e Combination with BX61 BX61WI D When the transmitted light unit is not installed SAFETY GUIDE 888 Page 2 5 WARNING LABELS Laser Safety Warning Labels e Combination with BX61 BX61WI CAUTION CL ASS 35 Anger ACHAT ION WHEN OP I ENTER FE TO Tine n mane t Ane SAFETY GUIDE _____f_____ 2 6 Page WARNING LABELS Laser Safety Warning Labels e Combination with 1X81 e Combination with 1X81 SAFETY GUIDE ee ee er Page 2 7 WARNING LABELS Laser Safety Warning Labels e Combination with 1X81 SAFETY GUIDE OOOO ZZ 2 8 Page WARNING LABELS aser Safety Warning Labels e Common for
10. an Standards Association CSA SV SVT SJ or SJT 3 X 18AWG OLYMPUS OLYMPUS CORPORATION Shinjuku Monolith 3 1 Nishi Shinjuku 2 chome Shinjuku ku Tokyo Japan OLYMPUS LIFE SCIENCE EUROPA GMBH Postfach 10 49 08 20034 Hamburg Germany OLYMPUS AMERICA INC 3500 Corporate Parkway P O Box 610 Center Valley PA 18034 0610 U S A OLYMPUS SINGAPORE PTE LTD 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 OLYMPUS AUSTRALIA PTY LTD 31 Gilby Road Mount Waverley Victoria 3149 Australia OLYMPUS LATIN AMERICA INC 5301 Blue Lagoon Drive Suite 290 Miami FL 33126 U S A Printed in Japan 200810 1 1
11. combinations with BX61 BX61WI and IX81 SAFETY GUIDE Page 2 9 WARNING LABELS Laser Safety Warning Labels e Common for combinations with BX61 BX61WI and 1X81 Tea SAFETY GUIDE SSN 2 10 Page WARNING LABELS Laser Safety Warning Labels e Common for combinations with BX61 BX61WI and IX81 eet eae e Common for combinations with BX61 BX61WI and 1X81 SAFETY GUIDE Page 2 11 WARNING LABELS Laser Safety Warning Labels Common for combinations with BX61 BX61WI and IX81 EN Ex m u Dur 5 i ES SAFETY GUIDE SSN 2 12 Page WARNING LABELS Laser Safety Warning Labels e Common for combinations with BX61 BX61WI and IX81 SAFETY GUIDE 4888 o o oo_ _ o Page 2 13 WARNING LABELS Laser Safety Warning Labels SAFETY GUIDE O 2 14 Page WARNING LABELS Laser Safety Warning Labels CAUTION CLASS 38 LASER RADIATION WREN OPEN AVOID EXPOSURE TO THE BEAN e Combination with BX61 BX61WI m MEE e Combination with IX81 SAFETY GUIDE ee o Page 2 15 WARNING LABELS Other Warning Labels 2 2 Other Warning Labels 5 AAN CAUTION Hot surface e Common for combinations with BX61 BX61WI and IX81 SAFETY GUIDE ff 2 16 Page WARNING LABELS Other Warning
12. cted light from the specimen may enter your eyes which is extremely hazardous e Never attempt to remove any of the units while the laser beam is output during laser scanning the laser beam may be output from the system and cause an extremely hazardous situation If you want to remove each unit refer to our service representatives since it is defined as the service activities 1 2 Transmitted lamp housing ex U LH100 Transmitted light fiber cable Removal from Transmitted Detector Unit CO a irr c H dq 4 Cube turret of the reflected light fluorescence unit 4 5 6 7 8 For detailed operation of lamp replacement see the manual of the REFLECTED LIGHT FLUORESCENCE ATTACHMENT Revolving nosepiece Objective Condenser Differential interference slider NN ARK ARK mm A Nc Excitation filter spectral filter and barrier filter Inside the scan unit SAFETY GUIDE 06b ST Page 1 5 SAFETY CAUTION e When you do not attach a objective to a the revolving nosepiece for objectives please be sure to attach a cap e Do not touch the stage while the Z motor is applied Otherwise the stage may move and pinch your hand or finger which is hazardous e If the system is disassembled for example opening a cover with a tool the ability of the product and safety cannot be assured Never attempt to disassemble the equipment e Do not bend or pull the laser fiber cable with an excessive force or step on it If th
13. e laser fiber cable is damaged the laser light may leak outside it and cause an extremely hazardous situation Should such an event occurs immediately turn off the laser power and contact your local Olympus representative e The air outlet of the laser cooling fan blows out warm air Do not place a flammable or non heat resistant object near the heat from the air outlet e Connect the ground correctly Otherwise Olympus can no longer warrant the electrical safety and performance of the equipment e Observe Electric Safety Standard of your country for power cable If the proper cord is not used product safety and performance cannot be guaranteed e Do not plug or unplug a cord or cable while the power is supplied Make sure that the main switch is set to O OFF before plugging or unplugging a cord or cable e The power cord may be melt by the heat of hot parts at 60 C or more such as the lamp housing etc if the cord comes in contact with them There is potential danger of electric shock Distribute the power cord at an enough distance from hot parts e The absolute alcohol used to clean the optics is highly flammable Take special care in handling against fire main switch ON OFF etc Room ventilate is recommended while you use absolute alcohol SAFETY GUIDE wf 1 6 Page SAFETY CAUTION pp O e The power supply connector to the HeNe laser is clamped with special hardware which should never be removed in
14. ensions mm Weight SDM HQ ion coating Barrier Filter Hamamatsu Photomultiplier R7400 94 W x 99 5 D x 144 H 2kg Page SPECIFICATIONS Specitications of Units Specifications Non Confocal Detector for SU FV10 OPD Additional Scan Unit FV10 ASU PC Interface Board FV10 PCIB Personal Computer FV10 PCSET Display Device Mounting on the SU external port to detect non Confocal fluorescence Number of Channel 2CH User can access and change the filter cube manually Wavelength Filter cube SDM Barrier Filter selection Hamamatsu Photomultiplier R7400 270 W x 73 D x 145 H Weight 3 7kg Mounting on the SU external port and lighting the selected laser beam at an arbitrary position of a sample separately from LSM observation Construction Scanning device 2 galvano meter mirrors point scanning Pupil Projection Lens Same as the main scanner Tube Lens common use with the main scanner Laser shutter with the Inter lock function Laser Introduction Port Standard 1 port for FV10 FUR or FV5 FURUV Control Optional software for FV10 ASW and FV10PSU required 270 W x 77 D x 145 H W Weight 2 3kg 187 W x 92 D x 20 H i enh Custom made I F board Dimensions mm Weight 0 11kg PC AT compatible GB or more 80GB or more Graphic board ATI RADEON 9600 or 9800 Pro Monitor Display 1 or 2 possible Display Resolution XGA 1280X1024 or more Display color 32Bit Full co
15. f Laser beam being output the system would remains in the state of Laser emission being waited when the trigger is not active Therefore the laser is not emitted When the laser emission should be terminated with Trigger Input mode click Laser emission button and set the system to the state of laser stop When Key Assign mode is used and even if Laser emission button is set to the state of Laser beam being output the system would remain in the state of Laser emission being waited when the key is not pressed Therefore the laser is not emitted When the laser emission should be terminated with Key Assign mode click Laser emission button and set the system to the state of laser stop SAFETY GUIDE 4 9 Page 1 3 SAFETY CAUTION While the LED lamp on the FV10 SU is illuminated the laser beam is being output from the objective mount hole There is potential danger that the reflected light may enter your eyes or the scattered light may leak outside etc Take special care for operating Never look at the specimen while the laser scanning It indicates that laser is being emitted when two lamps are lit Two lamps FV10 SU with FV10 SUIX FV10 SU with FV10 SUBX If any one of the LED
16. hard disk should be at least of 300Mbyte Disable the screen saver by setting it to None before use With this software the screen saver has already been set to None at the factory Never delete the folder or modify its name which created in the hard disk of the computer in advance or the software would not be starting up o Never activate other softwares while this software is running or it might cause malfunction of this software SAFETY GUIDE eo OST Page 3 5 SPECIFICATIONS On This Volume This volume describes the specifications of the FLUOVIEW FV1000 system Please read this volume so that you can understand the system before use CONTENTS pe 1 SPECIFICATIONS 1 1 l 1 SPECIICALIONS OF Unless 1 1 1 2 Power CONSUMPTION ee eres eee eee erre nennen nnn 1 8 SPECIFICATIONS Specifications of Units 1 SPECIFICATIONS 1 1 Specifications of Units Specifications Polarized preserved fiber cable length 3 m Laser Combiner Optical fiber FV10 MCOMB S 2 cable FV10 FURBB Single port type FV10 MCOMB S 2 1 cable FV10 MCOMB D 2 2 cables FV10 MCOMB D 2 514 640 W x 364 504 D x 236 H Dual port type mm Weight MAX 40kg Intensity AOTF Operation continuous change 0 1 to 100 0 1 step adjustment Operating Normal Laser shut off during beam flying back Mode ROI Intensity can be defined for each region Laser line can be selected for each region Gas
17. hose directly connected to a low voltage power supply network which supplies buildings used for domestic purposes Class B Equipment for use in domestic establishments and in establishments directly connected to a low voltage power supply network which supplies buildings used for domestic purposes This system is applied Class A Some interference may occur if this system is used in domestic location The system configuration of the Class A is as follows SPECIFICATIONS Page 1 7 SPECIFICATIONS Power Consumption 1 2 Power Consumption As shown below some of the units consume a large amount of power Be sure to check the power capacities of the power outlets before use Microscope Power Supply Power Supply Control Box Unit Unit UCB FV10 PSU FV10 PSU AC100 120V 220 240V 4A max 2A max AC100 120V 220 240V 4A max 2A max Monitor Monitor Reflected Fluorescence Light AC115V 2 8A max Power Supply Unit Europe Area U RFL T 200 AC230V 1 6A max AC100 120V 220 240V Power Supply Unit for 2A max 1A max the Laser combiner FV10 MCPSU Air Compressor AC90 132V 180 264V HeNe G Laser 6 5mA max 6 5mA max FV10 LAHEG230 2 Power Supply Unit e Power consumption of devices depend upon their destinations G Laser AC115V 0 4A max FV10 LAHEG230 Use any of these items Power Supply Unit AC230V 0 2A max AC100 120V 220 240V LD559 Laser Power 0 3A max
18. ification Mark Mark JET JQA TUV Belgium CEBEC Netherlands KEMA K ae UTE SEV Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or embossed Harmonization Black Red Yellow Thread Approval Organization Marking May be located on jacket or Length of color section in mm insulation of internal wiring Bax mes Yetow Comite Electrotechnique Belge CEBEC CEVEC lt HAR gt 10 30 10 Verband Deutscher Elektrotechniker VDE e V Pr fstelle lt VDE gt lt HAR gt Instituto Italiano del Marcio di Qualita IMQ IEMMEQU lt HAR gt British Approvals Service for Electric Cables BASEC BASEC lt HAR gt SEMKO AB Svenska Elektriska Materielkontrollanstalter SEMKO lt HAR gt Osterreichischer Verband fur Elektrotechnik OVK lt OVK gt lt HAR gt Danmarks Elektriske Materielkontrol DEMKO lt DEMKO gt lt HAR gt National Standards Authority of Ireland NSAI lt NSAI gt lt HAR gt 30 30 50 Norges Elektriske Materiellkontroll NEMKO NEMKO lt HAR gt 10 10 70 Asociacion Electrotecnica Y Electronica Espanola AEE lt UNDE gt lt HAR gt Hellenic Organization for Standardization ELOT ELOT lt HAR gt Instituto Portugues da Qualidade IPQ np lt HAR gt re Schweizerischer Elektro Technischer Verein SEV lt HAR gt Elektriska Inspektoratet SETI lt HAR gt re Underwriters Laboratories Inc UL SV SVT SJ or SJT 3 X 18AWG Canadi
19. lamps is illuminated solely while activating the FLUOVIEW software there may be a system failure Stop operating and contact your local Olympus representative SAFETY GUIDE eee 1 4 Page SAFETY CAUTION e Do not expose your hand or finger to the laser beam output from the objective mount hole objective tip or condenser lens or your skin may be damaged Also never attempt to output the laser beam outside the system by inserting a mirror or a similar object in the light path Otherwise the laser beam may enter your eyes which is extremely hazardous e Ifthe device is breaking down the laser beam may illuminate under the objective Do not put your hand in under the objective When you place a specimen on the stage you should pull out the stage place a specimen on it and move the specimen toward under the objective with the stage movement knob To take the specimen off you should also pull out the stage with the stage movement knob e Place the specimen horizontally on the stage If the specimen inclines the laser beam may reflect around the microscope which is extremely hazardous e Do not incline the specimen under the objective Otherwise the laser beam may reflect which is extremely hazardous e Do not remove the cube turret or the revolving nosepiece for objectives while the software is running e Do not set or remove the specimen while the laser beam is output during laser scanning Otherwise the refle
20. le light laser combination CLASS IIb CDRH LASER LIGHT AVOID EXPOSURE TO BEAM CLASS SB IEC60825 1 1993 A1 1997 A2 2001 CLASS Ib LASER PRODUCT CDRH CLASS 3B LASER PRODUCT 1EC60825 1 1993 A1 1991442 2001 50mW MAX CW 400 700nm Laser products of Class 3B are products that can only be used under control of laser safety officer Assign a laser safety officer and ensure safety in accordance with the instruction given by the said laser safety officer In IEC 60825 1 Safety of laser products Part 1 Equipment classification requirements and user s guide it requires safety preventive measures for users of laser products Before using read IEC 60825 1 of the latest edition carefully and then use the products after enforcing adequate measures Unless the safety measures indicated in IEC 60825 1 are provided our company cannot insure safety of the product since it may cause exposure of dangerous laser emission SAFETY GUIDE o Page 1 1 SAFETY CAUTION Safety Notes Take care regarding the following points during operation When the displayed function window Acquire panel shows the following figure the laser beam is being output from the objective mount hole There is potential danger that the reflected light may enter your eyes or the scattered light may leak outside etc Take special care for operating Never look at the specimen while the laser scanning Shows the laser beam being output
21. lor 16 770 000 colors a Windows XP Professional English CPU CPU entium4 2GHz or higher ep SPECIFICATIONS Page 1 5 SPECIFICATIONS Specifications of Units Specifications Software Main Acquisition Scanning Setting Features Image size Scanning Speed ZoomIN Panning etc FV10 ASW Hardware Setting FV10 SW Automatic Settings and Manual Settings PMT Sensitivity Automatic Setting Real time image operation Karman filter Peak detecting image processing Image Display Image size 128 128 2048 2048 Max channels 6CH image Image display Side by side Image Display Overlaying Display Trimming Tiling Step by step display slow motion and continuous motion Display for Series e g X T Icon for each image type RGB Table LUT RGB setting Virtual colors y setting Comment insert Graphic Pattern Text Scale etc Image Filtering Processing Average Low pass High pass Sobel Medial 2D laplacian edge enhance binary Image Processing Operation between images Arithmetic Logic Operation Image Analysis Bird view intensity Histogram intensity Perimeter Area Measurement Time series Measurement etc 3D Processing 3D Animation Section Display Display Others PAPP protocol processor for routine or complex experiment Time course lon Measurement Trigger IN OUT function Operating environment e Indoor use e Altitude Maximum 2000m e Ambien
22. ment is used in a manner not specified in this manual the safety of the user may be imperiled In addition the equipment may also be damaged Always operate the equipment following this user s manual SAFETY GUIDE es 3 4 Page CAUTION FOR SYSTEM OPERATION Computer and Software 3 7 Computer and Software The computer data may be destroyed by accident Be sure to back up the computer data as required e Olympus cannot assume liabilities for any auxiliary damage including compensation for loss of computer data resulting from use of or inability to use this product e The computer used with this system has already been set up with Microsoft Windows This operating system should be backed up and the backup retained carefully by the user Olympus does not provide backup support For details on the computer and Microsoft Windows also refer to the manuals provided with them e Quality assurance for this system is provided at ex factory basis Be advised that any operating failure or disordered function due to Environment Setting Change BIOS change or installation of other software by user is not covered by the warranty e When the remaining available hard disk space becomes small the data processing rate may drop drastically or errors may tend to occur To prevent this delete unnecessary data files frequently For the data file deletion procedure refer to the manuals provided with Microsoft Windows Free space of
23. nce every month Due to the properties of gas laser the laser life becomes shorter when it is not used for a long period than when itis used continuously e To stabilize the laser beam output leave the system for warm up after turning the laser power ON then use it All lasers should be warmed up for more than 30 minutes before use 3 5 Care and Storage e When the microscope is not in use be sure to set the main switch to O OFF After checking that the lamp housing has been cooled down place the dust prevention cover on the microscope for storage Never leave stains or fingerprints on the lenses and filters Should a glass component be stained simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper or clean gauze If a lens is stained with fingerprints or oil smudges wipe it gauze slightly moistened with commercially available absolute alcohol Since the absolute alcohol is highly flammable it must be handled carefully Be sure to keep it away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched on or off Also remember to always use it only in a well ventilated room e To clean a part wipe with a soft cloth moistened with a little drop of diluted neutral detergent Do not use organic solvents that will deteriorate the surface coating and plastic parts 3 6 Caution If the equip
24. nual Hardware Manual e FV1000 FV10 ASW User s Manual Quick Start Also we have prepared one service manual for this system as below Technical personnels who perform the service require to take the service training e FV1000MPE FV1000 Service Manual Reproduction copying or duplication of a part or all of this software and manual is prohibited Page 1 Registered Trademarks Registered Trademarks Microsoft Microsoft Windows Excel for Windows are registered trademarks of Microsoft Corporation Other brand names and product names are trademarks or registered trademarks of their respective owners FLUOVIEW MANUAL CONFIGURATION The FLUOVIEW system uses two manuals including this Users Manual and the on screen manual built into the software Online Help The User s Manual is composed of the two following volumes and subject matter SAFETY GUIDE Describes notes and cautions on using the FLUOVIEW system and on types of warning labels 1 SAFETY CAUTION ee pte Er ET ert EN 1 1 2 WARNING LABELS nada 2 1 3 CAUTION FOR SYSTEM OPERATION een 3 1 SPECIFICATIONS Describes the specifications of the FLUOVIEW system FSP EG I ICA NON Sisa 1 1 NOTATIONS IN THIS MANUAL This manual complies with the following notations lt gt Notation of Caution Notes and Tips Notation Description Caution to prevent injuries to the user or damage to the A product including surrounding objects aer Indica
25. ope adapter FV10 SUBX FV10 SUIX FV10 SUWI Microscope Adapter FV10 SUBX FV10 SUIX FV10 SUWI Prepared 3 ports for laser introduction Visible UV option IR more than 700nm Detector Choice for SU 3CH HQ ion coating Filter Type 2CH Spectrometer 1CH Filter Type Optional SU port available for twin scanner or 2CH non confocal detector Point optical scanning system with 2 galvano meter mirrors Device Scanning mode XY scanning Pixels 64 64 4096 4096 Aspect ratio 1 1 4 3 or Arbitrary ratio Scanning Speed Pixel duration 2us 5ms 9 steps Dimension Time Z Ramda combine freely Multi dimensions Line Type Straight line incl Rotation Free line T Z Ramda Number of lines 1 32500 Lines Free factor combination Line Scan Point Scan Field of view 18 FN 1 50 X 0 5x step Optical Path Motorized Path Switching owitching LSM FL Reflected Light Illuminator External Optional Port Filter Cube Mount conventional cubes on 4 positions out of 6 on the turret One Turret position is dedicated to the LSM path IX81 Motorized 6 position BX61 Motorized 6 position BX61WI Manual 6 position Dimensions 324 W x 487 5 D x 110 H FV10 SUIX mm 324 W x 603 7 D x 110 H FV10 SUBX 324 W x 603 7 D x 110 H FV10 SUWI Weight 10 98kg with FV10 SUIX FV10 SU 12 90kg with FV10 SUBX 11 18kg with FV10 SUWI SPECIFICATIONS 1 2 Page SPECIFICA
26. order to unplug the connector Even after the laser power switch is set to OFF prevent electric shock by not touching the connector because the laser head may still be charged e _ The outer remote interlock connector is equipped for all lasers Current Rating 70mA Voltage Rating DC 24V To prevent electric shock or fire be sure to consult your local Olympus representative before handling the outer remote interlock connector Remote interlock connector of laser PSU Backside Panel e The laser tube used with this system should always be disposed of in accordance with your local industrial waste treatment regulations When disposal is required please consult your local Olympus representative e Also refer to the section described about safety of the manual of microscope e Usage note of the external PMT unit OPD BXD IXD When image acquisition is not executed uncheck the Ch check box of the Ch group box on the Image Acquisition Control window roo SN HV Gi al Ch check box If the check box is left checked under the circumstance it is light in the room it may damage PMT SAFETY GUIDE Page 1 7 SAFETY CAUTION Safety Symbols The following symbols are found on the system Study the meaning of the symbols and always use the system in the safest possible manner Explanation Indicates that the surface becomes hot and should not be touched with bare hands Otherwise burns may resul
27. t Before use carefully read the user s manual Improper handling could result in injury to the user and or damage to the equipment Indicates that the voltage is so high Take special care to prevent electric shock Indicates that the laser beam is applied Take special care in handling Indicates that the main switch is ON Indicates that the main switch is OFF SAFETY GUIDE ese 1 8 Page 2 WARNING LABELS 2 1 Laser Safety Warning Labels The kind of Labels attached on the laser system depends on the laser combination you have Only visible laser system s label 1 2 3 2 1 1 Warning Label 1 LASER LIGHT AVOID EXPOSURE TO BEAM CLASS Ib LASER PRODUCT CDRH CLASS 38 LASER PRODUCT 1EC60825 1 1993 A1 1997 A2 2001 5 OmW MAX CW 400 700nm Attaching Position e Combination with IX81 Label 1 as shown The right hand side of a Scan Unit SAFETY GUIDE 888 Cocococooooooooo u ccoo COo Page 2 1 WARNING LABELS Laser Safety Warning Labels 2 1 2 Aperture Label AVOID EXPOSURE LASER RADIATION 13 EMITTED FROM THIS APERTURE Laser emits from the place with the above labels and indicated with arrow e Combination with BX61 I Objective or revolving nosepiece hole AVOID EXPOSURE i EM LASER f ma T TED Enz RADIATION 15 AU PORA THI s HIS APERTURI e Combination with BX61 BX61WI 2 Condenser lens SAFET
28. t temperature 18 C to 28 C Performance guarantees Maximum relative humidity 80 for temperatures up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F 60 at 37 C 99 F to 50 relative humidity at 40 C 109 F e Main supply voltage fluctuations not to exceed 10 of the nominal voltage e Pollution Degree 2 in accordance with IEC 60664 e Installation Over voltage Category II in accordance with IEC 60664 Storage environment e Ambient temperature 10 C to 40 C 77 F to 149 F Relative humidity 10 to 90 SPECIFICATIONS 1 6 Page SPECIFICATIONS Specitications of Units This device complies with the requirements of both directive 89 336 EEC concerning electromagnetic compatibility and directive 73 23 EEC concerning low voltage The CE marking indicates compliance with the above directives The directives are applied based on the conformance to the requirements of technical standards The standards below are used so as to verify the compliance with the directives Safety EN61010 1 2001 2 Edition Safety requirements for electrical equipment for measurement control and laboratory use Electromagnetic Compatibility EN61326 1997 A1 1998 A2 2001 Electrical equipment for measurement control and laboratory use EMC requirements EN61326 defines two categories according to the location for use Class A Equipment suitable for use in establishments other than domestic and t
29. tes details of caution to prevent damage or deterioration of products 1 TP Hint or one point advice for user reference 2 Page SAFETY GUIDE On This Volume This volume describes caution and notes for safe use of the FLUOVIEW FV1000 system Be sure to read this volume completely before use lf the system is operated in a manner not specified by this manual the safety of the user may be impaired IN addition the system may also be damaged Always operate the system as outlined in this user s manual CONTENTS pe 1 SAFETY CAUTIONZN 1 1 2 WARNING LABELS 2 1 2 1 Laser Safety Warning Labels 2 1 AN kabel tee een Deui 2 1 21 2 APEnuUre Lael eek 2 2 2 1 3 Protective Housing Labels WWW nennen nnne 2 5 2 2 Other Warning Labels ceres eee 2 16 3 CAUTION FOR SYSTEM OPERATION 3 1 3 1 Transportation of Equipment eere 3 1 3 2 Treatment of System 1 eee oom 3 1 3 9 Handling PrecautiOn Sanam 3 1 3 4 Recommendations for Use oo oo mo 3 3 3 5 Care and SITO 3 4 A na NN PD 3 4 3 7 Computer and Software oo oom 3 5 SAFETY CAUTION 1 SAFETY CAUTION N This product has been specified as a laser product of one of the following classes according to the combined laser 1 Visib
30. uired please consult your local Olympus representative e The mercury burner used with the illumination device of this system should always be disposed of in accordance with local industrial waste treatment regulations When disposal is required please consult your local Olympus representative 3 3 Handling Precautions This system is comprised of precision equipment Handle it with care Don t subject it to shock e Do not bend or pull the laser fiber cable with excess force or step on it for this may lead to considerable deterioration of performance e Once the reflected light power is switched OFF do not switch it ON again for at least 10 minutes Otherwise the life of the mercury burner may be shortened e Do not expose the laser head to cool air from an air conditioner etc Otherwise the operation will be unstable e Absolutely avoid condensation of dew of the laser tube for this will lead to explosion of the tube When the laser tube has been located in a cold environment for a long period do not heat the room until the laser operation has started to prevent condensation Any laser should be subjected for 8 hours of continuous aging every month when it has been left unused for a month or more If aging is not performed troubles which may affect the laser service life may occur such as instability or stoppage of laser oscillation SAFETY GUIDE eo Page 3 1 CAUTION FOR
31. wer Supply Unit for the Laser combiner FV10 MCPSU Microscope Upright type Inverted type Transmitted Light Detector FV10 TD Epi Fiber Illumination Unit FV10 FIR Fiber Port for Fluorescent Detection FV10 FPT Additional 4 channel fluorescent detector FV10 OP4CH SPECIFICATIONS 1 4 Specifications Dimensions mm 182 W x 358 D x 434 2 H 100 120 220 240V AC 4 0 2 0A max 50 60Hz 9 3kg 95 W x 210 D x 297 H Power consumption Weight Dimensions mm 100 120 220 240V AC 2 1A max 50 60Hz 4 3kg BX61 BX61WI BX62 BX62WI Power consumption Weight Models Objectives Models Objectives Z Drive System UIS series objectives IX81 UIS series objectives Motorized Focusing Module Microscope frame built in Moving step 0 01 um During Z series scanning the Focusing Module is directly controlled by FV10 PSU for faster frame rate via the Bandle fiber onto the transmitted light illuminator part with the microscope frame Motorized Switching for Transmitted Light Detector Transmitted Light Illumination Detector Lamp house Dimensions mm Weight Fiber connection from Lamp house to SU Provided with motorized shutter Motorized switching between LSM path and Illuminator path Dimensions mM 218 4 W x 180 D x 210 H Nen 4 48kg iber connector type FC Fiber core diameter 100 125um NA 0 2 Wavelength Selection Detector Dim
32. y be prevented Bring Microscope Configuration window and enter value in Near Limit text box on Focus tab in Microscope Configuration dialog box See Online Help Setting and Control of Microscope Main Unit Setting Microscope Main Unit About Focus tab e To prepare for an unexpected happening power switch cord should be turned off disconnect easily The system should be in a situation to be turned off disconnect the power switch the power cord from inlet or wall outlet easily anytime e If you want to use the simple anti vibration table select the one which is endurable the weight 70kg of the equipment SAFETY GUIDE es 3 2 Page CAUTION FOR SYSTEM OPERA TION Recommendations for Use e The proper conditions to operate lasers Multiline Ar laser HeNe laser and Semiconductor Laser are at a room temperature of 18 to 28 degrees C and at humidity of 30 to 80 96 To extend the service life of this system avoid using it in the following environments e In a place with excessive dust or dirt Dust and dirt taken in the oscillator may lead to failure Clean the system and peripherals periodically to avoid ball of dust e In a place where the temperature is low or high or where the humidity is high This includes for example a place that is near a water tap hot water heater room humidifier heater stove and a place subject to water drops e Ina place where humidity changes suddenly When a cold room
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