IOP:004.4 IOP:004.4A IOP:004.3B IOP:004.10C IOP:004.0D
Contents
1. SAFETY No special safety precautions PROCEDURES I Preparation of the Trilogy Fluorometer for use A With the unit turned off insert the green optical module into the base for the sample adapter B Turn on the On Off switch located on the back of the Trilogy Verify that the display becomes active and shows the module selection screen C Opt for the selected green module and touch OK on the confirming screen D When the display indicates the Home screen you are ready to use Trilogy in the Raw mode measurements are relative or calibrate Trilogy to make quantitative measurements II Calibration of the Fluorometer A With a known standard 1 On the home screen touch Calibrate to begin a calibration sequence 2 Select Run New Calibration 3 Select the unit of measurement PPB 4 Insert the calibration blank and touch OK 5 Enter the concentration for the first standard If doing a multi point calibration use standards in order of increasing concentration 6 Follow the screen prompt indicating that the standard should be inserted then touch OK 7 After the calibration is complete either select Proceed with Current Calibration or select Enter More Standards in which case return to 5 above 8 Save calibration for future use optional 9 Confirm successful completion of the calibration by measuring the previously used standard The disp2. B Allow the instrument to operate for 15 minutes G Assure that the emission and excitation filters are installed See Components 11 C 2 D Insert aperture slide if required E Place cuvette with blank solution or distilled water into the cuvette holder J MAINTENANCE I The cuvette used for the blank is matched to the cuvette s used during the sampling 1 Zero the instrument with the blank cuvette 2 Additional cuvettes used for sampling are matched to the blank sample cuvette within 1 fluorescence unit Set Span fully clockwise and adjust Zero knob until display indicates 000 Replace blank solution with sample to be measured If the display remains blank reduce Gain until a number is displayed If display remains blank on lowest Gain setting install aperture slide Read and record the fluorescence on the Dye Study data form Appendix M General Maintenance and Repair A Instrument is kept clean and out of dusty areas or areas with temperature and humidity extremes The dust cover is placed on the instrument when not in use Fuse Replacement 1 Turn off instrument and remove cable from power outlet 2 Remove power cable from back of instrument 3 Slide plastic cover to left exposing fuse holder 4 Locate black lever marked FUSE PULL Swing lever out and to the left 5 Remove bad or blown fuse 6 For 100 and 120 VAC voltages replace fuse with 2 0 amp fuse 7 Push lever back into place 8
3. 2 Sample Compartment II IH Instrument Components A Controls and indicators Model 450 1 Gain Turning clockwise to higher readings increases the gain of the instrument increases sensitivity of the instrument to read lower fluorescence emission The opposite effect occurs when turning gain counterclockwise 2 Zero This control is used to zero the instrument response This eliminates the sample background fluorescence before measuring samples 3 Span This control is used in conjunction with the gain control to display the concentration of a standard 4 Display Provides the instrument reading of fluorescence B Cuvette holder 1 The standard cuvette holder supplied with the instrument accommodates a 12mm X 75mm tube 2 To insert tube lift the cover from the front and insert the tube until it touches the bottom of the cell C Interchangeable Filter Holder 1 Inside of the cuvette holder are two machine slotted grooves designed to hold the emission and excitation filters 2 The emission filter is installed in the bottom groove and the excitation filter is installed in the groove on the left 3 Both filters are utilized to measure the relative fluorescence of Rhodamine WT Dye D Aperture slide 1 The aperture slide is located inside the cuvette holder at the top 2 The aperture slide reduces the sensitivity of the instrument when sample fluorescence is too high Operation A Turn on power switch back panel of instrument
4. Slide plastic cover to the right and reconnect power cord to instrument and power outlet Lamp Replacement 1 Turn instrument off and remove cable 2 Remove Cover a Remove two screws in lid of the cuvette holder with an Allen wrench and remove lid b Turn instrument over and remove the five recessed screws holding the cover in place c Turn the instrument upright and remove the cover 3 Locate the lamp within the black lamp housing Remove the two small screws holding lamp with a Phillips screwdriver 4 Remove the lamp and disconnect the two leads from the board 5 Clean the new lamp with lens tissue and acetone 6 Install the new lamp replace the screws and connect the two leads to the board The lamp is pre aligned and requires no further alignment 7 Replace the cover Il Troubleshooting A Little can be done by the operator to repair a malfunction in the Model 450 fluorometer 1 The Model 450 has no user replaceable or adjustable components other than the lamp and fuse 2 Tf the following steps do not solve problem contact dealer service representative B Front panel does not illuminate 1 Turn power off and remove electrical cord 2 Remove and inspect fuse Replace if necessary see fuse replacement section 3 Reinsert electrical cord and turn instrument on to verify proper operation C Front panel illuminates source lamp does not 1 Replace lamp See lamp replacement section D Front panel and source il
5. Standard Operational Instructions P N 998 0025 REV A This procedure has been reviewed and approved by the undersigned representatives of the U S Fish and Wildlife Service and Fisheries and Oceans Canada N L3 IES da REVIEWED APPROVED bat teen DATE 2 12 14 Field Supervisor U S REVIEWED APPROVED ARE o DATE 2 12 4 Division Manager Canada ATTACHMENT Calibration Log Stream Cal Std conc Solid Remarks ug L standard A IOP 004 0D Trilogy Laboratory Fluorometer IOP 004 0D U S Fish and Wildlife Service April 16 2008 Marquette Biological Station 3090 Wright Street Marquette Michigan 49855 U S A and U S Fish and Wildlife Service Ludington Biological Station 229 South Jebavy Drive Ludington Michigan 49431 U S A and Fisheries and Oceans Canada Sea Lamprey Control Centre 1219 Queen Street East Sault Ste Marie Ontario P6A 2E5 Canada INSTRUMENT OPERATING PROCEDURE INSTRUMENT Fluorometer MODEL Trilogy Laboratory Fluorometer MANUFACTURER Turner Designs SERIAL AND DEPARTMENT NOS Model number Property number Identifying number 7200 000 SLCC 720000190 06 12 06 12 PRECAUTIONS POTENTIAL INTERFERENCES Use a clean cuvette for all readings The Trilogy is sensitive sample residue may cause errors Use a lense cleaning cloth when using plastic Methacrylate cuvettes Fluorescence is temperature sensitive read the blank standard and all samples at the same temperature
6. IOP 004 4 IOP 004 4A IOP 004 3B IOP 004 10C IOP 004 0D Fluorometers IOP 004 4 Model 450 Fluorometer IOP 004 4 U S Fish and Wildlife Service April 16 2006 Marquette Biological Station 3090 Wright Street Marquette Michigan 49855 U S A and U S Fish and Wildlife Service Ludington Biological Station 229 South Jebavy Drive Ludington Michigan 49431 U S A and Fisheries and Oceans Canada Sea Lamprey Control Centre 1219 Queen Street East Sault Ste Marie Ontario P6A 2E5 Canada INSTRUMENT OPERATING PROCEDURE INSTRUMENT Fluorometer MODEL Model 450 MANUFACTURER Barnstead Thermolyne Sequoia Turner SERIAL AND PROPERTY NOS Model number Property number Identifying number 450 MBS 904980824984 1212 PRECAUTIONS POTENTIAL INTERFERENCES See instrument manual SAFETY No special safety precautions PROCEDURES I Installation A Place the instrument on a vibration free laboratory counter Allow two inches behind panel for air circulation and power cord B Assure that the proper fuse is installed in the voltage selector board i e 2 0 Amp fuse for 100 120 VAC E Connect power cord to instrument and to outlet SEQUOIA TURNER CORPORATION MODEL 450 DIGITAL FLUOROMETER 4 DISPLAY CUVETTE Model 450 Fluorometer 1 GAIN SWITCH 2 ZERO 3 SPAN FIG 3 1 SEQUOLA TURNER Model 450 Digital Fluorometer 7 Top of cuvette holder 6 EXCITATION FILTER EMISSION FILTER FIG 3
7. If the unit is moved from a cold to a hot humid area the unit may fail to operate correctly Leave instrument on and the condensate will evaporate in about 15 to 20 minutes E Little can be done by the operator to repair a malfunction in the Model 10 000 Fluorometer It is recommended that the unit be returned to a service technician for repair REFERENCE Operating and Service Manual for the Model 10 Series Fluorometers This procedure has been reviewed and approved by the undersigned representatives of the U S Fish and Wildlife Service and Fisheries and Oceans Canada REVIEWED APPROVED_ eb J ART DATE 2 9 12 Field Supervisor U S IOP 004 3B Model 10 AU 005 CE Fluorometer IOP 004 3B U S Fish and Wildlife Service January 12 2012 Marquette Biological Station 3090 Wright Street Marquette Michigan 49855 U S A and U S Fish and Wildlife Service Ludington Biological Station 229 South Jebavy Drive Ludington Michigan 49431 U S A and Department of Fisheries and Oceans Sea Lamprey Control Centre 1219 Queen Street East Sault Ste Marie Ontario P6A 2E5 Canada INSTRUMENT OPERATING PROCEDURE INSTRUMENT Fluorometer MODEL Model 10 AU 005 CE MANUFACTURER Turner Designs SERIAL AND PROPERTY NOS Model number Property number Identifying number PRECAUTIONS POTENTIAL INTERFERENCES No known interferences SAFETY No special safety precautions PROCEDURES I Installation A Place the instrument o
8. Ludington Michigan 49431 U S A and Department of Fisheries and Oceans Sea Lamprey Control Centre 1219 Queen Street East Sault Ste Marie Ontario P6A 2E5 Canada INSTRUMENT OPERATING PROCEDURE INSTRUMENT Fluorometer MODEL 8000 MANUFACTURER Turner Designs SERIAL PROPERTY AND DEPARTMENT NOS ves ose st mes o iPS 8000 002 MBS 800451 2197 a 8000 010 MBS 800626 2193 ae m s sesso ss SS PRECAUTIONS POTENTIAL INTERFERENCES Do not use glass or quartz cuvettes Use only lens cleaning tissues or cloth on plastic cuvettes a TM or Use a clean cuvette for all readings The Aquafluor is sensitive sample residue may cause errors Fluorescence is temperature sensitive read the blank standard and all samples at the same temperature High background or blank values may reduce sensitivity Do not use this instrument for dye dilution studies SAFETY No special safety precautions PROCEDURES I Preparation of the Aquafluor Fluorometer for use A Turn on the instrument by pressing ON OFF button The instrument will countdown from five after which it is ready to use B If RHOD doesn t appear in lower left of screen press the A B button This toggles between the Rhodamine and Turbidity channels II Calibration of the fluorometer A With a known standard 1 Press the STD VAL button 2 Use the up and down arrow buttons to adjust the readout to the standard value Holding the
9. button down activates scrolling When done press ENT or ESC to return to home screen 3 Press the CAL button then the ENT button 4 Insert a blank close cover and press ENT Be sure the cuvette is at least half full gt 2 mL of sample is free of bubbles and is clean and dry Wait until prompted before moving to the next step Always orient the cuvette in the same direction when inserting it into the instrument 5 Insert calibration standard and press ENT When calibration is complete Calibration Completed will be displayed press ENT If ENT is not pressed within 10 seconds the readout will ask if you wish to abort the calibration if not press the down arrow then ENT to accept the calibration 6 Insert the Secondary Solid Standard press either READ button and record attachment the result Slight variations in placement of the standard may result in variations in readings Itis recommended that 3 5 readings be taken while removing and reinserting the standard between readings The average of these readings is used for future checks of instrument stability and for recalibration B With the Secondary Solid Standard 1 If the instrument was previously calibrated with a known standard and the value of the Secondary Solid Standard was determined against that value you can recalibrate with the Secondary Solid Standard 2 Follow calibration directions II A 1 6 3 Enter the value found for the Secondary Solid Standard in
10. ets the basic sensitivity of the unit It requires adjustment if the optical filters are changed 2 Blank Is used to compensate for the residual or blank fluorescence corresponding to zero concentration of the unknown 3 Span Is used to calibrate the panel meter in the concentration of the unknown If the Blank control is properly set the Span control may be adjusted without affecting the setting of the blank control 4 Range Auto Man Switch When the control is in the Auto position the instrument selects the appropriate range for measuring the fluorescence of the sample When the control is in the Man manual position ranges are changed only with the Step switch 5 Range light indicators These indicate the selected sensitivity B Cuvette system 1 The model 10 000 comes with a high volume continuous flow cuvette system installed and ready for operation 2 A single sample cell cuvette is used for measurement of individual samples II Calibration A Prior to use a 100 ug L Rhodamine WT standard solution is prepared with deionized water The instrument response is linear so a single standard is sufficient TOP 002 x and TOP 003 x B Turn unit on and wait 5 minutes for instrument to warm up C Unlock BLANK knob and set full counter clockwise D Unlock SPAN knob Set three turns counter clockwise from the knob s clockwise stop E Pour deionized water through the cuvette and adjust the BLANK control knob to
11. layed concentration should equal the same value used in step 5 above Optionally the secondary standard now could be adjusted to give the same reading for future calibrations or accuracy checks B Using the Secondary Solid Standard 1 Measure a dye solution of known value 100 ppb standard and note the Trilogy reading 2 Place the secondary standard in the Optical Module note the reading and if necessary turn the adjustment screw to produce the same displayed concentration as in step 1 Turning the adjustment screw clockwise decreases the displayed concentration 3 The secondary standard can now be used for future calibrations or to check the instrument for accuracy prior to measurement of unknown samples IH Analyzing unknown samples A Samples are collected either through hand sampling hand or more routinely through the use of automatic water samplers Natural fluorescence is present in stream water so the fluorescence of water from the site to be sampled without Rhodamine is measured first to provide a background reading The background fluorescence blank is used for comparison to determine if Rhodamine dye is present or absent 1 Place a properly filled dry cuvette into the sample compartment Close the lid and press Measure Fluorescence The Trilogy will measure the sample for six seconds and report the average reading for the sample 2 The Trilogy reports data on the Home screen and displays the res
12. luminate display will not change 1 Perform installation procedure and operation procedure REFERENCE Operator s Reference Manual Model 450 Digital Fluorometer This procedure has been reviewed and approved by the undersigned representative of the U S Fish and Wildlife Service i REVIEWED APPROVED_ ed gt Field Supervisor U S DATE 2 7 7 IOP 004 4A Model 10 000 Fluorometer IOP 004 4A U S Fish and Wildlife Service January 12 2012 Marquette Biological Station 3090 Wright Street Marquette Michigan 49855 U S A and U S Fish and Wildlife Service Ludington Biological Station 229 South Jebavy Drive Ludington Michigan 49431 U S A and Fisheries and Oceans Canada Sea Lamprey Control Centre 1219 Queen Street East Sault Ste Marie Ontario P6A 2E5 Canada INSTRUMENT OPERATING PROCEDURE INSTRUMENT Fluorometer MODEL Model 10 000 MANUFACTURER Turner Designs SERIAL PROPERTY AND DEPARTMENT NOS Model number Property number Identifying number 10 000 MBS 000503 364182 Ss 10 000 MBS 000390 360269 PRECAUTIONS POTENTIAL INTERFERENCES No known interferences SAFETY No special safety precautions PROCEDURES I Installation A Place the instrument on a vibration free laboratory counter B Connect power cord to instrument and to outlet The unit will also run off a 12 volt battery if required II Instrument Components A Controls and indicators 1 Sensitivity S
13. menu screen 2 0 Set the concentration range to Auto Enter the concentration for your standard E If you want the instrument to subtract a blank set screen 2 12 to yes F Measure the blank to determine blank range The auto range mode will automatically select the appropriate range generally the lowest range Access Run Blank from screen 2 11 While on screen 2 11 wait until the reading is stable Then if the blank is less than 200 press lt 0 gt Remove the cuvette and set aside Run standard to determine the standard range access Run Standard screen 2 3 The auto range function will select the best standard range for the instrument that will ensure the optimum resolution Note the best accuracy and resolution is attained on low and medium ranges In all cases regardless of range selection the concentration of the standard must be within the linear range of the instrument for the dye being analyzed Return to the Home screen and analyze samples The dye concentration of each sample will be displayed digitally ug L IV Operation A Turn on power switch B Allow the instrument to warm up for 10 minutes Set the basic operating level E Calibrate the instrument see section III The calibration should be checked periodically to ensure accuracy precision and to detect drift D Pour a stream blank sample into a cuvette This reading can be subtracted from sample readings to compensate for background E Record the tempera
14. n a vibration free laboratory counter B Connect power cord to instrument and to outlet The unit will also run with power from a 12 volt battery if required II Instrument Components A Controls and indicators 1 Sensitivity Sets the basic sensitivity of the unit This procedure is done the first time before calibration and is locked in place 5 32 Allen screw 2 Blank Is used to compensate for the residual or blank fluorescence corresponding to zero concentration of the unknown 3 Span During calibration minor adjustments may be made to sensitivity with the span function 4 Auto ranging function Will select the appropriate instrument range for the standard and blank Instrument ranges high medium and low are in factors of 10 You also have the option of setting the instrument to manual and selecting the range for the blank and standard 5 Range alarm indicators These indicate the selected sensitivity B Cuvette system 1 A single sample cell cuvette is used for measurement of individual samples discrete sampling IH Calibration A The calibration process is completed only once the information is stored within the instruments memory Once calibrated drift is less than 0 5 B Prior to use prepare a standard that is 80 of the highest concentration that you may encounter but still within the linear range E Turn unit on and wait 10 minutes for instrument to warm up D Access the calibration
15. place of the known standard II Analyzing unknown samples A Samples are collected by either hand or more routinely through the use of automatic water samplers Natural fluorescence is present in stream water so the fluorescence of water from the site to be sampled without Rhodamine is measured first to provide a background reading Stream water with suspended solids must be filtered to remove the solids before measurement The background fluorescence is used for comparison to determine if Rhodamine dye is present or absent 1 If suspended solids are present in the stream water filter before measurement 2 Place a properly filled dry cuvette into the sample compartment Press either of the two READ buttons 3 The instrument will auto range measure and average the fluorescence for 5 seconds then display the reading at the top center of the display WAIT is displayed in the upper left corner of the display during the 5 second measurement period When WAIT disappears the next sample can be read MAINTENANCE A Minimal maintenance is required or possible Replacement of batteries is completed according to instructions found in the instrument manual A section on service troubleshooting is included in the instrument manual B All maintenance conducted on the instrument is recorded in the instrument log book REFERENCE Aquafluor Handheld Fluorometer and Turbidimeter User s Manual P N 998 0851 and Aquafluor Secondary Solid
16. read 0 0 on the maximum sensitivity scale Lock the BLANK knob Note Samples should be at least 400 mL to effectively flush the previous sample from the flow through cuvette F Select the appropriate range for the standard and pour the standard through the cuvette While pouring unlock the Span knob and adjust it until the panel meter indicates the concentration of the standard Lock the Span knob For the single cell cuvette model fill the cuvette and insert Record the temperature of the standard IV Operation A Turn on power switch B Allow the instrument to warm up for 5 minutes E Calibrate the instrument see section III D Pour a stream blank sample through the flow through cell or into a cuvette While pouring into the flow through cell unlock the blank control knob and adjust to zero on the maximum sensitivity scale X31 6 The volume of all the samples must be at least 400 mL to flush the flow through cuvette of the previous sample E Record the temperature of the samples for further temperature compensation if greater precision is needed MAINTENANCE I General Maintenance and Repair A Instrument is kept clean and out of dusty areas or areas with temperature and humidity extremes B All connections in the flow through cuvette must be kept air tight and hoses within 0 5 m of the flow cell must be opaque The sample compartment must be kept air tight to prevent the formation of condensation on the cuvette
17. ture of the samples for temperature compensation if greater precision is needed MAINTENANCE I General Maintenance and Repair A Instrument is kept clean and out of dusty areas or areas with temperature and humidity extremes Make sure cuvettes are clean clear and dry before use B The sample compartment should be air tight to prevent condensation on the cuvette If the unit is moved from a cold to a hot humid area the unit may fail to operate correctly Leave instrument on and the condensate will evaporate in about 15 20 minutes C Little can be done by the operator to repair a malfunction Itis recommended that the unit be returned to a service technician for repair I Documentation A Note all instrument malfunctions B Note all repairs conducted on the fluorometer REFERENCE Operating and Service Manual for the Model 10 AU 005 CE Fluorometer user s manual April 1999 Part Number 10 AU 074 Service department Turner Designs 845 W Maude Ave Sunnyvale CA 94086 This procedure has been reviewed and approved by the undersigned representative of the U S Fish and Wildlife Service A DI REVIEWED APPROVED Qu DATE_2 2 Field Supervisor U S IOP 004 10C Model 8000 Fluorometer IOP 004 10C U S Fish and Wildlife Service December 18 2012 Marquette Biological Station 3090 Wright Street Marquette Michigan 49855 U S A and U S Fish and Wildlife Service Ludington Biological Station 229 South Jebavy Drive
18. ults for the 20 most recent measurements If more than 20 readings are taken the oldest reading is overwritten Calibration is normally performed with standards made with distilled water so the stream blank must be subtracted from the reported value to ensure accuracy MAINTENANCE A Minimal maintenance is required or possible B Fluorescence troubleshooting is available in Appendix A of the User s manual REFERENCE Trilogy Laboratory Fluorometer User s Manual Trilogy Fluorometer Quick Start Guide This procedure has been reviewed and approved by the undersigned representatives of the U S Fish and Wildlife Service and Fisheries and Oceans Canada n 7 Y f k 6 A C REVIEWED APPROVED__ cu A J LUN DATE LCA Deu nl Division Manager Canada
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