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Axoclamp-2A Microelectrode Clamp Service Manual

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1. Set Bridge Balance to indicate 0 Using the DVM monitor TP12 V1 Thumbwheel on 050 0 continuous Repetitively switch from 050 0 to 050 0 Make a note of the total voltage change Using the DVM monitor 13 10 Trim RVT18 so change in 10 Vm is ten times change in V1 16 Vm DPM accuracy Repetitively switch from 050 0 to 050 0 Adjust trim pot inside Vm DPM to read same as DVM 17 offset Thumbwheel on 000 0 external On scope measure TP13 10 TP8 gnd at 20 mV div Set MEI Input Offset for 0 V On scope measure 12 1 TP8 gnd at 2 mV div Trim 16 for 0 V 18 CCS2 CMRR Bridge mode ME2 current clamp command off Plug x1 headstage into ME2 slot Connect 1 100 Hz square wave directly to the headstage input On scope measure 4 2 source voltage at 100 x V div Trim RVT2 for a null 19 12 CMRR On scope measure TP14 I2 TP17 gnd at 100 pV div Trim 4 for a null 20 ME2 COM gain Remove headstage Link pins 8 gnd and 11 V2a with a 22 Q resistor Using DVM monitor TP4 Vo2 Thumbwheel 100 0 continuous Destination ME2 Repetitively switch from 100 0 to 100 0 Trim RVT6 for 2 000 V difference 21 CCS2 offset Thumbwheel on 000 0 OFF On scope measure 4 1 TP17 gnd at 50 u V div Trim RVT3 for 0 V AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC Appendix
2. 1 0 REMOTE BUZZ 2 1 2300 001 0 ELMCH AXOCLAMP REMOTE BUZZ NONE 1 0 EA y AXON 3 10 2600 019 MECH REMOTE BUZZ NONE 1 0 y _ amp 101 5010 005 ALUM DIE CAST 2 25 X 1 38 X 1 12 TYPE _ NONE 1 0 POMONA 2417 Se 101 2270 023 C STUFFED REMOTE BUZZ 1 0 4 101 4100 019 1 CMOS DUAL MONOSTABLE 4538 01 1 0 GENELECT 045388 amp 102 4110 003 1C HC QUAD 2 INPUT NOR 74 02 u2 1 0 EA MOTOROLA MC74HCO2N amp 201 3600 005 SOCKET GOLD PLD DUAL LEAF 16 PIN 1 1 0 2 640358 4 6 202 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN 2 1 0 2 640357 4 4 301 3100 023 50 10 0 10 1 1 0 C322C104K5R5CA 4 302 3100 002 CAP CER DISC 50V 10 0 010 c2 1 0 EA MALLORY GE103M 4 350 3100 020 CAP CER ML 50V 0 2IN 0 10 1 0 EA 323 1042505 924 amp 601 4020 000 DIODE 1 SIGNAL 1N914 01 1 0 FAIRCHLD 14914 4 402 4020 000 DIODE 51 SIGNAL 1N914 1 0 FAIRCHLD 1N914 4400880 3500 0097 RES Fick 0 254 1 100PPM 10K RI 1 0 EA DALE 55 4 500 3500 009 RES FILM 0 254 1 100 10K R2 1 0 DALE CCF 55 amp 503 3500 009 RES FILM MET 0 25W 1 100PPM 10K R3 1 0 EA DALE 55 amp 506 3500 009 RES FILM MET 0 254 1 100PPM 10K R4 1 0 EA DALE CCF 55 1 8 505 3500 0
3. 5 22 12 offset On scope measure TP14 Im TP17 gnd at 100 4 V div Trim RVTS for 0 V 23 12 accuracy Thumbwheel on 100 0 continuous Repetitively switch from 100 0 to 100 0 Trim RVT24 for 1 000 on DPM select 12 24 ME2 Bridge Balance accuracy On scope measure TP15 V2 TP17 gnd at 2 mV div Plug in x1 headstage Ground via 10 MQ 1 or better Set Bridge Balance 2 to indicate 10 MQ Externally activate thumbwheel at 100 Hz Thumbwheel on 050 0 Trim RVT7 for a null 25 ME2 Bridge offset Switch step command off Use Offset control to center trace on screen Trim 15 so that trace does not shift as Bridge pot increased to maximum 26 V2 DPM accuracy Using the DVM monitor 15 2 Set Bridge Balance 2 to indicate 0 MQ Thumbwheel on 050 0 continuous Repetitively switch from 050 0 to 050 0 Adjust trim pot inside V2 DPM to read same as DVM 27 VC COM Set up TEVC at high gain requires two electrodes and an RC cell model Hold near 0 V Using the DVM monitor TP13 10 Vm Thumbwheel on 100 0 continuous Destination VC Repetitively switch from 100 0 to 100 0 Trim RVT19 for 2 000 difference AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 6 Appendix This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENT
4. berba HE 1 GENERAL Desde eR adir tente rad m POWER SUPPLY tet Lasst otim 1 REMOTE INPUT AND CONTROL 2 5V REFERENCE STEP COMMAND GENERATOR DESTINATION SWITCH 3 MICROELECTRODE 3 MICROELECTRODE 2 3 VOLTAGE CLAMP AND V1 CONTINUOUS 4 MAINTENANCE essa tts eade distet OM EDI fe EOS AC ERR 7 SPECIFICATIONS 9 DRAWING 1 CIRCUITS T DEN TT ADJUSTMENT PROCEDURE vidis TP ME 1 REPLACABLE PARIS i corel EE CLE AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC LIST OF ILLUSTRATIONS Page Fig 1 Timing Diagram CEPR OE 5 Fig 2 Differential Measurements on Oscilloscope Mc ELLE C B 7 CIRCUITS Remote Buzz T PNE RES 2 5V Reference Destination Switch ee 3 Current Command amp Headstage Drive Circuit ME1 4 Im Monitor Circuit PENES 5 Current Command amp Head
5. 25 10 00 R54 1 0 EA DALE CMF 55 T9 B 4 1055 3500 084 RES FILM 0 25w 1 100PPM 22 1 R55 1 0 DALE 55 4 1056 3500 002 RES FILM 0 254 1 100 108 56 1 0 DALE CCF 55 4 1057 3500 012 RES FILM MET 0 25W 1 100PPM 100K R57 1 0 EA DALE CCF 55 4 1058 3500 099 RES FILM 0 25M 1 100PPM 27K4 R58 1 0 EA DALE CCF 55 4 1059 3500 012 RES FILM 0 254 1X 100PPM 100K R59 1 0 DALE 55 4 1060 3500 222 RES FILM MET 0 25W 1 100 82K5 R60 1 0 EA DALE CCF 55 4 1061 3500 147 RES FILM MET 0 250 1 100PPM 39 2 R61 1 0 EA DALE CCF 55 4 1062 3500 039 RES FILM 0 25W 1X 100PPM 1 5 R62 1 0 EA DALE 55 6 1063 3500 039 RES FILM 0 254 1 100PPM 1 5 R63 1 0 DALE CCF 55 amp 1064 3500 012 RES FILM MET 0 254 1 100PPM 100K R64 1 0 EA DALE CCF 55 amp 1065 3500 012 RES FILM 0 254 1X 100PPM 100K R65 1 0 EA DALE CCF 55 4 1066 3500 057 RES FILM 0 254 1X 100PPM 18K2 R66 1 0 EA DALE CCF 55 4 1067 3500 003 RES FILM 0 25W 1 100PPM 100R R67 1 0 DALE CCF 55 amp 1068 3500 015 RES FILM MET 0 25W 1 100 1M R68 1 0 EA DALE CCF 55 6 1069 3500 042 RES FILM MET 0 25W 1 100PPM 15K R69 1 0 EA DALE CCF 55 4 1070 3500 123 RES FILM MET 0 25W 1 100PPM 3328 R7O 1 0 EA DALE CCF 55 4 1071 3560 006 RES FILM 0 254 1
6. BICCVERO 20 21370 amp 1409 3800 002 TERMINAL PCB TEST EYELET TPO 1 0 BICCVERO 20 21370 4 1410 3800 002 TERMINAL PCB TEST EYELET TP10 1 0 EA BICCVERO 20 21370 4 1411 3800 002 TERMINAL PCB TEST EYELET TP11 1 0 EA 20 2137D 4 1412 3800 002 TERMINAL PCB TEST EYELET TP12 1 0 EA BICCVERO 20 21370 4 1413 3800 002 TERMINAL PCB TEST EYELET TP13 1 0 EA BICCVERO 20 21370 4 1414 3800 002 TERMINAL TEST EYELET TP14 1 0 BICCVERO 20 2137D 4 1415 3800 002 TERMINAL PCB TEST EYELET 15 1 0 BICCVERO 20 21370 4 1416 3800 002 TERMINAL PCB TEST EYELET TP16 1 0 EA BICCVERO 20 21370 6 1417 3800 062 TERMINAL PCB TEST EYELET 17 1 0 BICCVERO 20 21370 amp 1418 3800 002 TERMINAL PCB TEST EYELET 18 1 0 EA BICCVERO 20 21370 6 1419 3800 002 gt TERMINAL TEST EYELET 19 1 0 20 21370 4 1420 3800 002 TERMINAL PCB TEST EYELET M TP20 1 0 EA BICCVERO 20 21370 amp 1421 3800 002 TERMINAL PCB TEST EYELET 21 1 0 EA 20 21370 4 1422 3800 002 TERMINAL PCB TEST EYELET 0 TP22 1 0 EA BICCVERO 20 21370 4 1423 3800 002 TERMINAL PCB TEST EYELET 25 1 0 EA BICCVERO 20 21370 4 1601 3430 001 K PCB AXOCLAMP 2 MAIN NONE 1 0 EA 4 1602 3435 001 K SCH AXOCLAMP 2 MAIN NONE 1 0 EA AXON 3 201 2300 023 ELMCH FRONT PANEL AXOCLAMP 2 NONE 1 0 EA y AXON 6 1 3000 001 COMPONENT NOT PRESENT 301 1
7. DATE ENT 20 26 Im Vm OUTPUT BANDWIDTH KHZ 1 3 4412 R222 49 1 2K21 100m V V d J4 EXT COM 6185 RVTI9 8K25 2K R169 R170 20mv v 10K 3K32 J13 17 21 R184 R304 13 15 10K VR HOLDING POSITION 13 6 gt VC MEI ME2 St SHIFT 22 24 R303 R181 B25R 3 19 17356 V C301 02 5 2304 1 0 0014 0 010 GU LF356 qus R313A LF347B J13 5 1K21 10K LIN Ji3 11 10K LOG J13 20 2 A v Cw R175 J13 10 S315 562R 13 7 20 Q 200 PARON 03 02 89 gt 6 INSTRUMENTS INC PARON 04 11 89 NEXT NEXT ASSY USED ON MME VOLTAGE CLAMP 2270 024 2 AND Vi CONTINUOUS gt en 9 06 08 90 18 14 scare 0016_ ster 7 or 10 REMSION RECORD bare 46 11 13 173478 R189 MS 221R gt gt V BATH OUT R189 4758 46 3 10 15V gt BATH C34 D CONNECTOR 1U TANT 10 TANT J6 5 12 15V gt gt R198 4758 n3 18 916 gt V BATH 1 26 V BATH 2 26 ME2 4415 BATH OUT MRTUAL GND D CONNECTOR DISPLAY SELECT 28 DISPLAY IVG 28 SELECT 12 25 j a PARTS UST 03 02 89 pg AXON INST
8. FILM FILM FILM FILM FILM FILM FILM FILM MET MET 2 4 BILL OF MATERIAL BY ITEM REPORT 0 254 0 25 Axon Instruments Inc 0 1 25PPM 10 00 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 CARBON CMPSN 6 254 0 254 0 254 0 25w 0 259 0 254 0 25 0 254 0 25 6 254 0 25 0 254 0 254 0 254 1 1 1 1 1 1 1 1 1 1 1 1 1 1 100PPM 100 100 100 100 100 100 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100 100 100 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100 0 254 5 10M 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 100PPM 499R 392R 4K75 18 2 221R 4758 1K 18K2 221R 100R 100K 20K 274R 221R 49 9 10K 4998 100k 10K 6K81 10K 100R 56R2 121R 4K75 4K75 3K32 82R5 18K2 2K21 2K21 392R 22R1 22R1 4998 475 90K 10K 1 4 75 100K 100R 47K5
9. GOLD PLD DUAL LEAF 14 1 XA19 1 0 AMP 2 640357 4 920 3600 004 SOCKET GOLD PLD DUAL LEAF 14 PIN 20 1 0 2 640357 4 4 921 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 PIN 21 1 0 2 640463 4 4 922 3600 004 SOCKET GOLD PLD DUAL LEAF 14 PIN XA22 1 0 EA 2 640357 4 amp 923 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 PIN 23 1 0 EA 2 640465 4 4 924 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 PIN 24 1 0 EA 2 640463 4 4 925 3600 000 SOCKET GOLD PLO DUAL LEAF 8 PIN XA25 1 0 AMP 2 640463 4 6 926 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 26 1 0 2 640463 4 6 927 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 XA27 1 0 EA 2 640463 4 4 928 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 PIN XA28 1 0 2 640463 4 6 929 3600 000 SOCKET GOLD PLD DUAL LEAF 8 PIN XA29 1 0 AMP 2 640463 4 6 930 3600 000 SOCKET GOLD PLD DUAL LEAF 8 PIN 1 0 2 640463 4 4 931 3600 000 SOCKET 1 GOLD PLD DUAL LEAF 8 XA31 1 0 2 640463 6 2 6 9320 3600 000 SOCKET GOLD PLO DUAL LEAF 8 PIN 2 1 0 2 640463 4 6 951 3600 005 SOCKET IC GOLD PLD DUAL LEAF 16 PIN XU1 1 0 EA 2 640358 4 4 952 5566 005 SUCKET IC GOLD PLD DUAL LEAF 15 Xue 1 0 2 640358 4 4 953 3600 004 SOCKET IC GOLD PLD DUAL LEA
10. 0 1 25PPM 10 00 R23 1 0 DALE 55 9 4 1024 3500 171 RES FILM 0 25W 1 100PPM 4998 R24 1 0 EA DALE CCF 55 4 1025 3500 018 RES FILM MET 0 25W 1 100PPM 1281 R25 1 0 EA DALE CCF 55 amp 1026 3500 147 RES FILM 0 25W 1X 100 39K2 R26 1 0 DALE CCF 55 4 1027 3500 084 RES FILM 0 25W 1 100PPM 22 1 R27 1 0 EA DALE CCF 55 amp 1028 3500 156 RES FILM 0 25W 1X 100PPM 475R R28 1 0 EA CORNING RN60D4750F 6 1029 3500 009 0 254 1 100 10 R29 1 0 EA DALE CCF 55 4 1030 3500 177 RES FILM 0 25W 1X 100PPM 49 9 R30 1 0 EA 55 4 1051 3500 009 RES FILM 0 25W 1 100 10 R31 1 0 DALE 55 4 1032 3500 171 RES FILM MET 0 25W 1 100PPM 499R R32 1 0 DALE 55 4 1033 3500 042 RES FILM 0 250 1 100PPM 15K 33 1 0 DALE CCF 55 4 1034 3500 123 RES FILM 0 25W 1X 100 3328 R34 1 0 EA DALE CCF 55 7 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 7 Axon Instruments Inc 12 48 17 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 6 1035 3500 006 RES FILM 0 259 1X 100 1K R35 1 0 EA DALE CCF 55 amp 1036 3500 002 RES FILM MET 0 25W 1X 100 10R R36 1 0 DALE CCF 55 4 1037 3500 006 RES FILM MET 0 25W 1 100PPM 1K R37
11. 10K R305 1 0 BOURNS 35905 291 103 4 406 3400 017 POT TEN TURN WIREWOUND 5K R306 1 0 BOURNS 35905 291 502 4 407 3400 016 POT TEN TURN WIREWOUND 20K R307 1 0 BOURNS 35905 291 203 amp 408 3400 014 POT TEN TURH WIREWOUND 10K R308 1 0 BOURNS 35905 291 103 amp 409 3400 014 A POT TEN TURN WIREWOUND 10K R309 1 0 BOURNS 35905 291 103 4 410 3400 016 A POT TEN TURN WIREWOUND 20K R310 1 0 BOURNS 35905 291 203 4 411 3400 014 POT TEN TURN WIREWOUND 10K R311 1 0 EA BOURNS 35905 291 103 4 412 3400 016 A POT TEN TURN WIREWOUND 20K R312 1 0 BOURNS 35905 291 203 4 413 3400 007 SGL TURN PLSTC DUAL LOG LIN 10K R313 1 0 ALLNBRAD 25M319 4 421 3500 006 RES FILM MET 0 256 1X 100 1K R321 1 0 EA DALE CCF 55 4 422 3500 126 RES FILM MET 0 25W 1X 100PPM 3K32 R322 1 0 DALE CCF 55 4 423 3500 009 RES FILM MET 0 25W 1X 100PPM 10K R323 1 0 EA DALE CCF 55 4 424 3500 129 RES FILM MET 0 254 1 100PPM 33K2 R324 1 0 EA DALE CCF 55 4 425 3500 012 RES FILM 0 25W 1X 100PPM 100K R325 1 0 EA DALE 55 4 426 3500 006 0 25 1 100 1 326 1 0 EA DALE CCF 55 amp 427 3500 126 RES FILM 0 254 1 100 3K32 R327 1 0 DALE 55 amp 428 3500 009 RES FILM MET 0 250 1X 100PPM 10K R328 1 0 EA DALE CCF 55 6 429 3500 129 RES FILM 0 254
12. 2281 R136 1 0 EA DALE CCF 55 4 1137 3500 002 RES FILM 0 254 1 100 108 137 1 0 DALE CCF 55 m 1 0 EA DALE CCF 55 1138 3500 075 RES FILM MET 0 25W 1 100PPM 22R1 R138 C 9 4 2 6 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 9 Axon Instruments Inc Time 12 48 35 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 4 1139 3500 204 RES FILM 0 25 1 100PPM 6K81 R139 1 0 DALE CCF 55 amp 1140 3500 204 RES FILM 0 25W 1 100PPM 6K81 R140 1 0 DALE CCF 55 4 1141 3500 204 RES FILM 0 254 1 100PPM 6K81 R141 1 0 DALE CCF 55 4 1142 3500 204 RES FILM 0 25W 1 100 6K81 R142 1 0 EA DALE CCF 55 4 1143 3500 204 RES FILM 0 254 1 100PPM 6K81 R143 1 0 EA DALE CCF 55 4 1144 3500 204 RES FILM MET 0 254 1X 100 6K81 R144 1 0 DALE CCF 55 4 1145 3500 204 RES FILM MET 0 259 1X 100PPM 6K81 R145 1 0 EA DALE CCF 55 4 1146 3500 204 RES FILM 0 254 1X 100PPM 6K81 R146 1 0 EA DALE CCF 55 4 1147 3500 204 RES FILM 0 254 1 100PPM 6K81 R147 1 0 EA DALE CCF 55 4 1148 3500 204 RES FILM 0 25 1X 100PPM 6K81 R148 1 0 EA DALE CCF 55 6 1149 3500 204 RES FILM MET 0 25W 1 100 6 1 R149 1 0 DALE CCF 55 h 1150 3500 204 RES FILM 0 25W 1 100PPM 6K81 R150 1 0 DALE CCF 55 4 1151 3500 099 RE
13. 44 40 X 0 188 HEATSINt 10 0 EA 4C36 PSS 5 601 4220 002 IC VOLTAGE REG 15V TO 220 7815 1 1 0 EA FAIRCHLD UA7B15UC 5 602 4220 005 A IC VOLTAGE REG 15V TO 220 7915CT vR2 1 0 FAIRCHLD UA7915UC 9 603 4220 002 A IC VOLTAGE REG 15V TO 220 7815CT VR3 1 0 EA FAIRCHLO UA7815UC 5 604 4220 005 A IC VOLTAGE REG 15 TO 220 7915 VR4 1 0 EA FAIRCHLD UA7915UC 5 605 4220 001 IC VOLTAGE REG 5V 10 220 7805 VR5 1 0 EA MA7805UC 5 606 4030 003 DIODE ZENER SOOMW 15V 1N965A VR6 1 0 EA MOTOROLA 1N965A 5 607 4030 003 DIODE ZENER SOOMW 15V 1N965A VR7 1 0 EA MOTOROLA 1N965A 5 701 3800 002 TERMINAL TEST EYELET 1 1 0 EA BICCVERO 20 21370 5 702 3800 002 TERMINAL PCB TEST EYELET 2 1 0 20 21370 5 703 3800 002 TERMINAL PCB TEST EYELET 1 0 EA BICCVERO 20 2137D 5 704 3800 002 TERMINAL PCB TEST EYELET TP4 1 0 BICCVERO 20 21370 5 705 3800 002 TERMINAL PCB TEST EYELET TPS 1 0 EA 20 21370 5 706 3800 002 TERMINAL PCB TEST EYELET TP6 1 0 EA BICCVERO 20 21370 5 707 3800 002 TERMINAL PCB TEST EYELET TP7 1 0 EA BICCVERO 20 21370 5 708 3800 002 TERMINAL PCB TEST EYELET 1 0 BICCVERO 20 21370 5 709 3800 002 TERMINAL PCB TEST EYELET TPO 1 0 EA 20 2137D 5 710 3800 002 TERMINAL PCB TEST EYELET TP10 1 0 20 21370 5 71
14. 5 DRIVE UMLESS OTMDFMGE 55 0010 4 oF 10 REMSION RECORD fev oF DISPLAY SELECT 28 1 9 622 0 0014 R107 d 2K BRIDGE BALANCE R116 10K 0 1 412 12 412 9 R312 CAL REF 21 BATH R11 28 10K 0 1 End 10K 0 1 R113 0 1 Gd manna or SST 1 17 PARON 03 02 88 AXON INSTRUMENTS INC MARC _PARON 04 11 89 assy JME 2270 024 AXOCLAMP 2A 2 2 _ _ 13430 001 2 MAIN UNLESS i 06 08 90 18 10 na a sneer 5 oF 10 4 CAL REF 21 VIB 22 Potted 06 08 90 18 12 815 R210 100K 100K 15V RVT16 BRIDGE BALANCE J 5 7 410 11 ET A R105 R17 R16 4K75 1K R19 10K 0 1 4 R20 10K 0 1 X R18 1 10 R197 2218 315 4 REVISION RECORD Fe i SEE SHEET 1 mem je LII PARTS LIST om MARC PARON O3 02 85 MARC PARON PARON 04 11 89 11 89 3430 6001 SIZE INSTRUMENTS INC BRIDGE BALANCE MONITOR amp CONTINUOUS SCE 1D 8 V REVISION RECORD REY
15. BOURNS 4608X 101 103 5 105 3410 006 TRIM MULTI TURN CERMET TOP ADJUST 20K 1 1 0 BOURNS 3296 1 203 5 201 3100 021 CAP CER ML 50V 20 1UF c1 1 0 EA KEMET 330 105 505 5 202 3000 001 COMPONENT NOT PRESENT c2 1 0 EA 5 203 3100 020 CER ML 50V 0 215 0 10 c3 1 0 KEMET C323C10425U5CAC924 5 204 3100 020 CAP CER ML 50V 0 2IN 0 10 C4 1 0 KEMET C323C10425U5CAC924 5 301 4020 003 DIODE SCHOTTKY 1N6263 105711 01 1 0 EA H P 185711 5 401 3220 009 CONN INS DISPL SOCKET 0 100 GOLD 8 PIN P1 1 0 PANDUIT 100 24 8 0 5 402 3220 017 CONN COVER FOR 0 100 INS DISPL 8 PIN P1 1 0 EA PANDUIT SCC100F 8D 5 403 3210 000 CONN EDGE 5 PIN FOR THUMBWHEEL SWITCH J2 1 0 CHERRY 260 123 5 406 3210 000 CONN EDGE 5 PIN FOR THUMBWHEEL SWITCH 43 1 0 EA CHERRY 260 123 5 405 3210 000 CONN EDGE 5 PIN FOR THUMBWHEEL SWITCH J4 1 0 EA CHERRY C2G0 P125 5 406 3210 000 CONN EDGE 5 PIN FOR THUMBWHEEL SWITCH J5 1 0 EA CHERRY 260 125 5 407 3210 000 CONN EDGE 5 PIN FOR THUMBWHEEL SWITCH 46 1 0 EA CHERRY 260 123 5 501 3700 000 SWITCH THUMBWHEEL 199 9 BCD SWT1 1 1 0 CHERRY 75 17 5 601 3600 006 SOCKET GOLD PLD DUAL LEAF 18 PIN _ 1 0 2 640359 4 5 602 3600 000 SOCKET GOLD PLD DUAL LEAF 8 PIN XA2 1 0 EA 2 640463 4 2 5 701 3430 004 0 THUMBWHEEL TO VOLTAGE CONVERTER N
16. THRMLLY 22258 amp 801 3410 002 POT TRIM MULTI TURN CERMET TOP ADJUST 10K RVT1 1 0 EA BOURNS 3299 1 103 4 802 3410 004 POT TRIM MULTI TURN TOP ADJUST 200R RVT2 1 0 BOURNS 3296 1 201 4 803 3410 006 TRIM MULTI TURN CERMET TOP ADJUST 20K RVT3 1 0 BOURNS 3296 1 203 4 804 3410 008 TRIM MULTI TURN CERMENT TOP ADJUST 500R 1 0 EA BOURNS 3296 1 501 4 805 3410 006 POT TRIM MULTI TURN CERMET TOP ADJUST 20K RVTS 1 0 BOURNS 3296Y 1 203 4 806 3410 009 POT TRIM MULTI TURN CERMET TOP ADJUST 5K RVT6 1 0 BOURNS 3296 1 502 4 807 3410 009 POT TRIM MULTI TURN CERMET TOP ADJUST 5K RVT7 1 0 BOURNS 32967 1 502 4 808 3410 004 POT TRIM MULTI TURN CERMET TOP ADJUST 200R RVT8 1 0 BOURNS 3296Y 1 201 4 809 3410 006 POT TRIM MULTI TURN CERMET TOP ADJUST 20K RVT9 1 0 BOURNS 3296 1 203 4 810 3410 004 POT TRIM MULTI TURN CERMET TOP ADJUST 2008 RVT10 1 0 BOURNS 3296 1 201 4 811 3410 006 POT TRIM MULTI TURN CERMET ADJUST 20K RVT11 1 0 BOURNS 3296 1 203 6 812 3410 009 POT TRIM MULTI TURN CERMET TOP ADJUST 5K RVT12 1 0 EA BOURNS 3296 1 502 4 813 3410 010 TRIM MULTI TURN CERMET TOP ADJUST 50K RVT13 1 0 EA BOURNS 3296Y 1 503 4 814 3410 010 POT TRIM MULTI TURM CERMET TOP ADJUST 50K RVT14 1 0 EA BOURNS 3296Y 1 503 4 815 3410 006 POT TRIM MULTI TURN CERMET TOP ADJUST 20K RVT15
17. 0 EA 4 2 3730 002 SWITCH ROT 0 51 2 2 5 5302 1 0 ALCOSW 2 5 3 3730 003 SWITCH ROT 0 514 1 2 10 303 1 0 ALCOSW MRS 1 10 4 4 3730 003 SWITCH ROT 0 514 1 2 10 304 1 0 ALCOSW MRS 1 10 24 5 3730 003 SWITCH ROT 0 51N 1P 2 10T 305 1 0 EA ALCOSW MRS 1 10 4 6 3730 007 A SWITCH ROT 0 751N NON SHORTING 2P6T S306 1 0 EA 7 3750 000 SWITCH TGL MINI 2P ON NONE ON 307 1 0 EA ALCOSW 206 4 8 3720 005 SWITCH MOM ILLUM 1P2T 5308 1 0 EA UNIMAX 01 121 S y 370 005 PB RECI MOM ILLUM 309 1 0 EA THO1 121 4 10 3720 005 RECT MOM ILLUM TP T 310 1 0 EA THO1 121 S4 11 3720 005 SWITCH PB RECT MOM ILLUM 1P2T 311 1 0 EA UNIMAX THO1 121 2 12 3750 006 SWITCH TGL MINI 2P ON OFF ON 312 1 0 EA ALCOSW MTA 206P 4 B 3750 005 SWITCH TGL MINI 1 ON OFF ON 313 1 0 EA ALCOSU 1065 4 14 3730 008 SWITCH ROT 0 7514 NON SHORTING 4P3T 314 1 0 GRAYHILL 718Y233309 4 15 3730 007 SWITCH ROT 0 751N NON SHORTING 2 6 315 1 0 EA 4 16 3750 006 SWITCH TGL MINI 2P ON OFF ON 316 1 0 EA ALCOSW MTA 206P 4 17 3720 000 SWITCH PB MINI MOM 1P2T 317 1 0 8121SY2GE 12 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 12 Axon Instruments Inc Time 12 49 00 Level Item Component Rev Description Ref Qty UM P
18. 1 command 10 x H nA V Ext ME 2 microelectrode 2 command 10 x H nA V Input Impedance 22 kQ Max Input Voltages 30 for voltage clamp commands 60 V for current clamp commands CALIBRATION SIGNAL A pulse equal in magnitude to the setting on the thumbwheel switch is superimposed on the voltage and current outputs for the duration of a HIGH control signal on the CAL ACTIVATE input AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC SPECIFICATIONS Page 13 BATH POTENTIAL COMPENSATION Signal recorded by bath headstage or by an external amplifier is automatically subtracted from the intracellular measurements If bath potential is not measured the system automatically reverts to using OV as the reference potential Standard headstages HS 2 work as bath headstages when plugged into the bath headstage connector VIRTUAL GROUND CURRENT MEASUREMENT A VG 2 virtual ground headstage can be plugged into the connector provided The current measured is the sum of all currents into the preparation The correct operation of the AXOCLAMP is not affected by the use or nonuse of virtual ground current measurement REMOTE Logic HIGH control signals activate BUZZ and CLEAR of each microelectrode and select between BRIDGE DCC SEVC and TEVC modes 15 pin connector MODEL CELL model cell is provided with the AXOCLAMP 2A Electrodes are 50 MQ cell is 50 500 pF A switch grounds the electrodes directly BAT
19. 10 15 CER DISC 50V 10X 150P TANT 25V 10U TANT 25V 10U COMPONENT NOT PRESENT CAP CAP CAP CAP CAP CAP CAP CAP CAP FILM 10X 100V 0 001U FILM 10X 100V 0 001U CER DISC 50V 10X 33P CER DISC 50V 10X 15P FILM 10 100V 0 001U TANT 35V 10 CER ML 50V 0 21 0 1U ALUM ELCTLT RDL LEAD 25V 100U CER ML 50V 0 2IN 0 10 u12 u13 04 015 a1 Q4 05 Q6 a7 CRD1 CRD2 CRD3 CRD4 1 VR2 VR3 VRS D1 D2 03 ci c2 5 c6 c7 c8 c9 c10 c11 12 13 14 15 16 17 18 19 c20 21 22 C23 C24 c25 C26 C27 C28 9 a h A BP VP Barrera Be ch ch amp eA eh eA 4 A hb o o Qty UM Mfg ANLGDEVC Date 02 13 90 Time 12 47 36 Manufacturer Part AD7512DIJN MOTOROLA MC140198CP AXON ANLGDEVC ADVFC32KN FAIRCHLD FAIRCHLD FAIRCHLD FAIRCHLD FAIRCHLD MOTOROLA SILCONIX TELCRYST TELCRYST TELCRYST TELCRYST MOSTEK MOSTEK MOSTEK MOSTEK FAIRCHLD FAIRCHLD FAIRCHLD FAIRCHLD MALLORY KEMET KEMET ARCO ARCO ARCO ARCO ARCO MALLORY KEMET KEMET ARCO MALLORY MALLORY MALLORY MALLORY MALLORY KEMET KEMET AXON ARCO ARCO MALLORY MALLORY ARCO KEMET KEMET ILLIN
20. 140288 4 59 4440 000 IC DARLINGTON DRIVER 9605 99 1 0 1 UA9665FC 6 60 4440 000 HEPT DARLINGTCN DRIVER 9665 u10 1 0 EA FAIRCHLD UA9665PC 6 61 4230 008 IC SWITCH JFET SPDT X2 NOT FOR NEW DESIGNS 011 1 0 EA ANLGDEVC AD7512D1JN 2 usr qad c4 p Page 2 101 102 103 104 105 106 107 201 202 203 204 301 302 303 304 305 351 352 353 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 _ 425 426 427 428 429 ey NE RARI ce MEE NU i t AE UE OE NEED ode d de dA RRR RR ie 4M GA dA dA de Ge dM 4A GM GM GA GM GM GA de qe GA GA GA dA dA GA dM GA dA dM RRR RRR dA 4230 008 4100 004 3000 001 4210 000 4620 000 4620 000 4620 000 4620 000 4620 000 4640 001 4610 001 4030 000 4030 000 4030 000 4030 000 4030 001 4030 001 4030 001 4030 001 4220 004 4020 000 4020 000 4020 000 3130 017 3100 020 3100 020 3130 001 3130 009 3130 000 3130 001 3130 009 3100 004 3160 001 3160 001 3130 006 3100 000 3100 000 3100 007 3100 003 3100 004 3160 001 3160 001 3000 001 3130 000 3130 000 3100 007 3100 003 3130 000 3160 000 3100 020 3120 006 3100 020 2 4 BILL OF MATERIAL BY ITEM REPORT Axon Instruments Inc Description
21. 3310 007 KNOB 14 5MM 0 25 BLK W LINE R313 1 0 EA ELMA 021 3520 4 607 3310 031 KNOB WING 14 5MM 0 125 BLK W LINE NONE 3 0 EA ELMA 023 3220 4 608 3310 010 KNOB WING 10MM 0 125 BLK W LINE 303 1 0 EA 023 2220 6 609 3310 010 KNOB WING 10MM 0 125 BLK W LINE 304 1 0 ELMA 023 2220 4 610 3310 010 KNOB WING 10MM 0 125 BLK W LINE 305 1 0 EA ELMA 023 2220 C 14 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 14 Axon Instruments Inc Time 12 49 19 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part amp 611 3310 029 KNOB WING 14 5MM 0 25 BLK W LINE S306 1 0 EA ELMA 023 3520 4 612 3310 029 KNOB WING 14 5MM 0 25 BLK W LINE 314 1 0 EA ELMA 023 3520 4 613 3310 029 KNOB WING 14 5 0 25 BLK W LINE 315 1 0 ELMA 023 3520 4 701 3310 021 KNOB CAP FOR 14 5MM BLK R301 1 0 ELMA 040 3020 4 702 3310 021 KNOB CAP FOR 14 5MM BLK R302 1 0 EA ELMA 040 3020 6 703 3310 021 KNOB CAP FOR 14 5MM BLK 305 1 0 ELMA 040 3020 4 704 3310 023 KNOB CAP FOR 14 5MM YELLOW R306 1 0 040 3050 amp 705 3310 025 KNOB CAP FOR 14 5MM BLUE R311 1 0 EA ELMA 040 3040 4 706 3310 022 KNOB CAP FOR 14 5MM RED R313 1 0 ELMA 040 3030 4 707 3310 021 KNOB FOR 14 5MM BLK 302 1 0 ELMA 040 3020 4 708 3310 018 KNOB CAP FOR 10MM BLK 5503 1 0 ELMA 040 1020 4 709 3310 018 KNOB CAP FOR 10M
22. CABLE FOR REMOTE BUZZ CABLE 8 306 OVERALL SHIELD 0 13 00 HS 2 BUSHING RUBBER FOR 0 22 CABLE CONN D SUB MALE SOLDER CUP 15 PIN CONN 0 SUB HOOD 15 STRAIN RELIEF 0 17 CABLE K 375 125 SWITCH CAP FOR MINI PB 0 375 BLACK SWITCH CAP FOR MINI PB 0 375 BLUE KNOB 10MM 0 125 BLK W LINE KNOB CAP FOR 10MM BLK MECH PANEL COVER ROMOTE BUZZ PANEL COVER REMOTE BUZZ PANEL ARTWORK COVER REMOTE BUZZ SCREW SHT MTL PAN 2 0 25 0 085 POMONA PACKAGING ZIP LOCK BAG 6 X 8 X 0 002 ACCESSORIES AXOCLAMP INCAND LAMP LAMP INCAND T 1 75 MDGT GRVD BASE 14V PACKAGING ZIP LOCK BAG 3 X 5 X 0 002 S U CLAMP 1 MODEL CELL ELMCH CLAMP 1 MODEL CELL CABLE GND LEAD FOR CLAMP 1 MODEL CELL PLUG 0 080 2MM SOLID GOLD BRASS PLUG 0 080 2MM SOLID GOLD BRASS WIRE PATCH 206 41 X 36 0 087 OD BLK TUBING HEATSHRINK POLYOLEFIN 6 X 0 125 BLK MECH CLAMP 1 MODEL CELL MACHINED PLUG FOR MODEL CELL 2MM MACHINED PLUG FOR MODEL CELL 2MM JACK 0 080 2MM GOLD BRASS NO INSUL BOX ALUM DIE CAST NO COVER HS 2 TYPE RES HI MEG FILM LOW CAP 2X 50M RES HI MEG FILM LOW CAP 2X 50M RES HI MEG FILM LOW CAP 2X 50M CAP CER DISC 50V 10 470P SWITCH TGL SEALED 1P ON NONE ON JACK 0 080 2MM GOLD BRASS NO INSUL MECH PANEL COVER CLAMP 1 PANEL COVER CLAMP 1 MODEL CELL PANEL ARTWORK CLAMP 1 MODEL CELL SCREM SHT MTL PAN 2 0 25 0 085 POMO
23. EA 2 640357 4 4 983 3600 004 SOCKET 1 GOLD PLD DUAL LEAF 14 PIN XRL3 1 0 EA 2 640357 4 6 984 3600 004 SOCKET IC GOLD PLO DUAL LEAF 14 PIN XRL4 1 0 2 640357 4 4 985 3600 004 SOCKET GOLD PLD DUAL LEAF 14 PIN XRL5 1 0 EA 2 640357 4 4 986 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL6 1 0 AMP 2 640357 4 4 987 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL7 1 0 2 640357 4 4 988 3600 004 SOCKET GOLD PLD DUAL LEAF 14 PIN XRL8 1 0 EA 2 640357 4 4 989 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL9 1 0 2 640357 4 6 990 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL10 1 0 EA 2 640357 4 4 991 3600 004 SOCKET GOLD PLD DUAL LEAF 14 PIN XRL11 1 0 EA 2 640357 4 amp 992 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL12 1 0 2 640357 4 amp 993 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL13 1 0 AMP 2 640357 4 4 1001 3500 009 RES FILM MET 0 25W 1X 100PPM 10K R1 1 0 EA DALE 55 4 1002 3500 012 RES FILM MET 0 25W 1 100 100K R2 1 0 EA DALE CCF 55 4 1003 3500 171 RES FILM MET 0 254 1 100 4998 R3 1 0 EA DALE CCF 55 4 1004 3500 204 RES FILM MET 0 25W 1 100PPM 6K81 R4 1 0 EA DALE CCF 55 4 1005 3530 001 RES HI MEG CARBON CMPSN 0 254 5X 10M R5 1 0 EA ALLNBRAD RCO7GF106J 4 1006 3500 138 RES FILM MET 0 25W 1 100PPM 3
24. REDRAWN amp UPDATED DWG PER AXON S CURRENT STD 30 3702 89 _____________ AXON INSTRUMENTS INC ENG MARC PARON 4 11 89 TE POWER SUPPLY 3430 005 AXOCLAMP 2A UMLEXS 3435 005 010 scale 005 01 sheet 106 1 REVISION RECORD sme orc REDRAWN amp UPDATED DWG PER AXON S 4 06 89 CURRENT STANDARD 303 UPGRADED 1 TO BECOME 10 NO CHANGES APPLIED 5 08 89 TO 5 DWG PER 370 REMOVED C2 NC PER ECO 506 5 07 00 ad 07525 MSB 44 41 4 5 4 6 4 7 5 4 5 5 5 6 95 7 46 4 46 5 COMPONENTS LOCATED BEHIND 1992 THUMBWHEEL SWITCH 3430 004 om on L BERNARD 8 05 12 87 INSTRUMENTS INC ARD B 05 11 87 gt T ASSY USED ON TLE rest assy SCH THUMBWHEEL _________ 2270 034 3450004 _ CONVERTER CL 3435 00418 _ NSCHNOO4 seer Tor 1 Si V Plotted 05 07 90 17 27 APPENDIX 1 ADJUSTMENT PROCEDURE ABBREVIATIONS CMRR common mode rejection ratio TP test point Vp p Volts peak to peak gnd ground H headstage current gain scope cathode ray oscilloscope
25. SCC100F 127 SCC100F 4D 1818 13 1822 33 1485 83 643A 7412 19 653A T amp 10 19 1411 4 2850 1220563 1353 106 025 5 1 220563 103 1353 106 025 5 103 VTL33S63 VTL33563 PDB C330C105M5USCA C330C105MSUSCA 16 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 16 Axon Instruments Inc Time 12 49 44 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 5 13 3120 003 CAP ALUM ELCTLT RDL LEAD 35V 2200U 13 1 0 INTCOMP 5 1 3100 021 CER ML 50V 20 1UF 14 1 0 330 105 505 5 15 3100 021 CER ML 50V 20 1UF C15 1 0 EA KEMET C330C105M5U5CA 5 16 3120 004 CAP ALUM ELCTLT RDL LEAD 16V 4700U C16 1 0 INTCOMP PDB 5 17 3000 001 COMPONENT NOT PRESENT 17 1 0 EA 5 18 3100 020 CAP CER ML 50V 0 211 0 10 18 1 0 EA C323C10425U5CAC924 5 101 3500 126 RES FILM MET 0 25W 1X 100 3K32 R1 1 0 EA DALE CCF 55 5 102 3500 054 RES FILM MET 0 254 1X 100 1 82 R2 1 0 EA DALE CCF 55 5 201 3220 005 CONN PCB 0 156 STRAIGHT W LOCK 4 PIN 41 1 0 EA PANDUIT MLSS156 4 D 5 202 3220 005 CONN 0 156 STRAIGHT W LOCK 4 PIN J2 1 0 PANDUIT MLSS156 4 D 5 203 3220 013 CONN INS DISPL SOCKET 0 156 4 PIN NONE 2 0 PANDUIT 156 22 40 5 204 3220 021 CONN COVER FOR 0 156 INS DISPL 4 PIN P2 1 0 EA PANDUIT SCC156F
26. SOCKET IC GOLD PLD DUAL LEAF 8 PIN 1 0 EA 2 640463 4 4 904 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 PIN XA4 1 0 EA AMP 2 640463 4 6 905 3600 001 SOCKET IC GOLD PLD 8 PIN ROUND 0 23 DIA XA5 1 0 EA ARIES 81 514 10 4 906 3600 001 SOCKET 1 GOLD PLD 8 PIN ROUND 0 23 DIA XA6 1 0 ARIES 8L 514 10 6 907 3600 001 SOCKET IC GOLD PLD 8 PIN ROUND 0 23 DIA XA7 1 0 ARIES BL 514 10 6 908 3600 000 SOCKET GOLO PLD DUAL LEAF 8 PIN XAB 1 0 EA 2 640463 4 amp 909 3600 000 SOCKET IC GOLD PUD DUAL LEAF 8 PIN XA9 1 0 EA AMP 2 640463 4 amp 910 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XA10 1 0 2 640357 4 4 911 3600 000 SOCKET GOLD PLD DUAL LEAF 8 XA11 1 0 2 640463 4 4 912 3600 001 SOCKET GOLD PLO 8 PIN ROUND 0 23 DIA 12 1 0 ARIES 81 514 10 amp 913 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 PIN XA13 1 0 EA 2 640463 4 4 914 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 14 1 0 EA 2 640463 4 4 915 3600 001 SOCKET GOLD PLD 8 ROUND 0 23 DIA 15 1 0 EA ARIES 8L 514 10 6 916 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 16 1 0 2 640463 4 amp 917 3600 004 SOCKET 1 GOLD PLO DUAL LEAF 14 PIN XA17 1 0 AMP 2 640357 4 4 918 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN 18 1 0 2 640357 4 4 919 3600 004 SOCKET
27. except that the case is grounded instead of driven Less than 10 uV peak to peak grounded input Available in 5 values specify two with order Select on basis of cell input resistance and maximum current capacity Imax x0 0001 for ion sensitive electrodes 0 01 for Rin greater than about 300 0 1 for Rin about 30 300 H x 1 standard for Rin about 3 30 x10 for about 300 to 3 These ranges are suggested for optimum performance Some overlap is allowable Imax 1000 x H nA 5 uV rms measured with a 10 kHz single pole filter in the measurement circuit 51 47 pV rms measured with a 10 100 source resistance and capacitance neutralization adjusted for a 10 1 kHz bandwidth and with a 10 1 kHz single pole filter in the measurement circuit Values are for H x0 1 HS 2L headstage 16 54 us for a voltage step applied to the input via a 10 100 MQ low capacitance resistor and 16 60 us for a current step into the same resistor Capacitance neutralization adjusted for zero overshoot Values are for H x1 0 1 AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 10 l SPECIFICATIONS Working Input Voltage Range 13 V for transients and steady state protected to 30 V Input Resistance 1014 1015 0 x 0 0001 see note 1013 0 x 01 1012 0 01 1010 1090 2 x 10 Note For the x0 0001
28. 0 Lag range ms 0 0 0 02 0 0 2 0 2 0 20 0 200 Anti Alias Filter Time constant range 0 2 100 us RMP Balance Indicators Equal brightness indicates voltage clamping will be at resting membrane potential Blank Stops clamp from responding to new inputs for the duration of a HIGH control signal the BLANK ACTIVATE input Used to reject stimulus artifacts Series Resistance Compensation Operates cSEVC mode Value set on Bridge potentiometer External input at 100 mV V can be used in TEVC mode AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 12 SPECIFICATIONS SAMPLING CIRCUIT Rate 500 Hz to 50 kHz Operates in DCC and dSEVC modes only Counter 3 digit display to 99 9 kHz max Blanked in continuous modes Sample Clock Logic level trigger output at the sampling rate Sample Acquisition Time 1 ps 10 V step to 0 1 INTERNAL COMMANDS Note Commands from all sources sum linearly Voltage Clamp Step Command 199 9 mV Set on thumbwheel switch Activated by a HIGH control signal on the STEP ACTIVATE input or by a front panel switch Voltage Clamp Holding Position Range 200 mV transmembrane potential Ten turn potentiometer Current Clamp Step Command 199 9 x nA Set on thumbwheel as above DC Current Command 100 H nA Ten turn potentiometers EXTERNAL COMMANDS Sensitivities Ext VC command 20 mV V Series resistance compensation 100 mV V Ext ME 1 microelectrode
29. 00 16 4 312 3220 020 CONN COVER FOR 0 100 INS DISPL 20 PIN P312 1 0 PANDUIT SCC100F 20C 13 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 13 Axon Instruments Inc Time 12 49 09 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 4 313 3220 020 CONN COVER FOR 0 100 INS DISPL 20 PIN P313 1 0 PANDUIT 5 100 20 4 314 3220 019 CONN COVER FOR 0 100 INS DISPL 16 PIN P314 1 0 PANDUIT 5 100 16 4 315 3220 017 CONN COVER FOR 0 100 INS DISPL 8 315 1 0 PANDUIT 5 100 80 4 316 3220 016 CONN COVER FOR 0 100 INS 01591 4 PIN P317 1 0 EA PANDUIT 5 100 40 amp 331 3210 001 CONN EDGE 2 X 10 0 156 EYELETS W MOUNTS P331 1 0 EA TEXMATE CN L10 4 332 3210 001 CONN EDGE 2 X 10 0 156 EYELETS W MOUNTS P332 1 0 EA CN L10 4 333 3210 001 CONN EDGE 2 X 10 0 156 EYELETS W MOUNTS P333 1 0 TEXMATE CN L10 amp 334 3210 001 CONN EDGE 2 X 10 0 156 EYELETS W MOUNTS P334 1 0 EA CN L10 amp 601 3400 014 POT TEN TURN WIREWOUND 10K R301 1 0 BOURNS 35905 291 103 amp 402 3400 001 SGL TURN PLSTC LOG 100 R302 1 0 ALLNBRAD 73J1G032P104A amp 603 3400 005 A POT SGL TURN PLSTC LIN 5K R303 1 0 EA ALLNBRAD 73416032P502U 4 404 3400 014 A POT TEN TURN WIREWOUND 10K R304 1 0 EA 5 3590s 291 103 4 405 3400 014 POT TEN TURN WIREWOUND
30. 00 CAP CER DISC 50V 10 10P c70 1 0 EA MALLORY GE100D 4 471 3130 001 CAP FILM 10 100V 0 01U c71 1 0 ARCO PRI 103 K 2A 4 472 3130 000 CAP FILM 10 100V 0 001U c72 1 0 EA ARCO PR1 102 K 2A ak 473 3100 020 CAP CER SOV 0 216 0 10 673 1 0 EA 325 1042505 924 4 474 3100 020 CER ML 59 0 215 0 10 C74 1 0 EA KFMET C323C104Z5U5CAC924 4 475 3100 020 CAP CER ML 50V 0 211 0 10 C75 1 0 323 1042505 924 4 476 3100 020 CAP CER ML 50V 0 21N 0 1U C76 1 0 KEMET 323 1042505 924 amp 477 3100 020 CER ML SOV 0 2IN 0 10 677 1 0 KEMET 3235 1042505 924 4 478 3120 000 CAP FILM 10 100 0 0010 78 1 0 EA ARCO PR1 102 K 2A 4 479 3130 018 CAP FILM PS 50V 2 5X 330P 79 1 0 MALLORY 5 1333 4 480 3130 000 CAP FILM 10 100V 0 0010 80 1 0 EA ARCO PRI 102 K 2A 4 1 0 EA C323C10425U5CAC924 481 3100 020 CAP CER ML 50V 0 21 0 1U C81 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 4 Axon Instruments Inc Time 12 47 53 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 4 482 3100 006 CAP CER DISC 50V 10 220P 82 1 0 EA MALLORY GE221K 4 501 3450 000 gt RELAY PCB MOUNT DIP FORM 1 5V RL1 1 0 EA CPCLARE PRMATCOSB 4 502 3450 000 RELAY PCB MOUNT DIP FORM 1C 5V RL2 1 0 EA CPCLARE 1 5 4 503 3450 0
31. 00 RELAY PCB MOUNT DIP FORM 1 5V RL3 1 0 CPCLARE 1 058 4 504 3450 000 RELAY PCB MOUNT DIP FORM 1C 5V RL4 1 0 CPCLARE PRMA1COSB 4 505 3450 000 RELAY PCB MOUNT DIP FORM 1C 5V RLS 1 0 EA CPCLARE PRMA1CO5B 4 506 3450 000 RELAY PCB MOUNT DIP FORM 1C 5v RL6 1 0 PRMA1COSB 4 507 3450 000 RELAY PCB MOUNT DIP FORM 1C 5 RL7 1 0 CPCLARE PRMA1CO5B 4 508 3450 000 RELAY PCB MOUNT DIP FORM 1 5V RL8 1 0 EA CPCLARE PRMA1C058 6 509 350 000 RELAY PCB MOUNT DIP FORM 1 5V RL9 1 0 EA PRMA1CO5B 4 510 3450 000 RELAY PCB MOUNT DIP FORM 1C 5V RL10 1 0 CPCLARE PRMA1CO5B 4 511 3450 000 RELAY PCB MOUNT DIP FORM 1C 5v RL11 1 0 CPCLARE 1 058 4 512 3450 000 RELAY PCB MOUNT DIP FORM 1C 5V RL12 1 0 CPCLARE 1 5 4 513 3450 000 RELAY PCB MOUNT DIP FORM 1C 5V RL13 1 0 CPCLARE PRMAICOSB 4 601 3220 000 CONN PCB 0 100 STRAIGHT GOLD 4 PIN 41 1 0 PANDUIT 55100 4 4 602 3220 001 CONN 0 100 STRAIGHT GOLD 8 42 1 0 PANDUIT MPSS100 8 D A 4 603 3200 005 CONN D SUB FEMALE 90 DEG W BRKT 15 43 1 0 117 DA 15SA 4 604 3200 005 CONN D SUB FEMALE 90 DEG W BRKT 15 PIN 44 1 0 AMP 117 DA 1554 4 605 3200 005 CONN D SUB FEMALE 90 DEG W BRKT 15 PIN J5 1 0 AMP 117 DA 15SA amp 606 3200 005 CONN D SUB FEMALE 90
32. 063 8 875 750 PCB STUFFED POWER SUPPLY AXOCLAMP 2 CAP ALUM ELCTLT RDL LEAD 63V 2200 CER DISC 500V 0 010 CAP TANT 25V 10U ALUM ELCYLT RDL LEAS 63V 2200 CER DISC 500 0 010 CAP TANT 25V 10U CER DISC 500v 0 010 CAP ALUM ELCTLT RDL LEAD 63V 33U CAP ALUM ELCTLT RDL LEAD 63V 33U CAP ALUM ELCTLT RDL LEAD 35V 2200U CAP CER ML 50V 20 1UF CAP CER ML 50V 20 1UF y gt gt e o e o e eb oh oA b e 4 c lA e oa oh oh gt AMP AMP AMP EF JOHN EF JOHN EFJOHN CONCORD AMPHENOL FELLER BUSSMAN BUSSMAN PANDUIT PANDUIT PANDUIT PANDUIT PANDUIT PANDUIT PANDUIT PANDUIT PANDUIT PANDUIT AXON y AXON AXON y AXON ELMA ELMA ELMA CONCORD CONCORD AXON HHSMITH AXON AXON HEYCO AXON MALLORY MALLORY KEMET MALLORY MALLORY KEMET MALLORY MALLORY MALLORY INTCOMP KEMET KEMET 227726 2 227726 2 227726 2 227726 2 227726 2 227726 2 227726 2 108 0907 001 108 0912 001 108 0912 001 07 9084 1 0312 117 155 5200 1 23 GDC 0 5 GDC 0 5 CE100F24 8 D A CE100F24 12 T A CE100F24 4 D A CE100F24 12 T A 100 24 4 0 SCC100F 8D SCC100F 121 SCC100F 4D
33. 1 100PPM 33K2 R329 1 0 EA CCF 55 4 630 3500 012 RES FILM 0 25W 1 100PPM 100K R330 1 0 EA DALE CCF 55 4 431 3500 051 RES FILM 0 259 1X 100 182R R331 1 0 DALE CCF 55 amp 432 3500 051 RES FILM MET 0 25W 1 100PPM 182R R332 1 0 EA CCF 55 amp 433 3500 090 RES FILM MET 0 250 1X 100PPM 2784 R333 1 0 EA DALE CCF 55 4 434 3500 012 RES FILM MET 0 25W 1 100PPM 100K R334 1 0 EA DALE CCF 55 4 501 3310 001 DIAL TURNS COUNTING 11N DIAM W BRAKE R304 1 0 EA BOURNS H 494 3 4 502 3310 601 DIAL TURNS COUNTING 11N DIAM W BRAKE R305 1 0 BOURNS H 494 3 4 503 3310 001 DIAL TURNS COUNTING 114 DIAM W BRAKE R307 1 0 BOURNS 494 3 4 504 3310 001 DIAL TURNS COUNTING 11M DIAM W BRAKE R309 1 0 EA BOURNS H 494 3 amp 505 3310 001 DIAL TURNS COUNTING DIAM W BRAKE R310 1 0 BOURNS 494 3 4 506 3310 001 DIAL TURNS COUNTING 1IN DIAM W BRAKE R308 1 0 EA BOURNS H 494 3 4 507 3310 001 DIAL TURNS COUNTING 1IN DIAM W BRAKE R312 1 0 BOURNS H 494 3 _ 4 601 2310 027 KNOB 14 5MM 0 25 BLK R301 1 0 EA ELMA 020 5520 4 600 1210 00 505 14 5 0 25 BLK R302 1 0 FLEA 021 5520 4 603 2310 007 KNOB 14 5MM 0 25 BLK W LINE R303 1 0 ELMA 021 3520 4 604 3310 027 KNOB 14 5MM 0 25 BLK R306 1 0 EA ELMA 020 3520 4 605 3310 02 KNOB 14 5 0 25 BLK R311 1 0 EA ELMA 020 3520 4 606
34. 1 0 BOURNS 3296Y 1 203 4 816 gt 3410 010 POT TRIM MULTI TURN CERMET TOP ADJUST 50K RVT16 1 0 BOURNS 3296Y 1 503 5 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 5 Axon Instruments Inc Time 12 48 91 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part amp 817 3000 001 COMPONENT NOT PRESENT RVT17 1 0 amp 818 3410 005 POT TRIM MULTI TURN CERMET TOP ADJUST 2K RVT18 1 0 EA BOURNS 3296Y 1 202 4 8197 3410 005 POT TRIM MULTI TURN CERMET TOP ADJUST 2K RVT19 1 0 BOURNS 3296Y 1 202 6 820 3000 001 NOT PRESENT RVT20 1 0 AXON 6 821 3410 006 TRIM MULTI TURN CERMET TOP ADJUST 20K 21 1 0 BOURNS 3296Y 1 203 6 822 3410 010 POT TRIM MULTI TURN CERMET TOP ADJUST 506 RVT22 1 0 BOURNS 3296 1 503 6 823 3410 008 POT TRIM MULTI TURN CERMENT TOP ADJUST SOOR RVT23 1 0 BOURNS 3296Y 1 501 amp 824 3410 001 POT TRIM MULTI TURN CERMET TOP ADJUST 1 RVT24 1 0 BOURNS 3296Y 1 102 6 825 3410 001 POT TRIM MULTI TURN TOP ADJUST 1 RVT25 1 0 BOURNS 3296Y 1 102 4 826 3410 006 POT TRIM MULTI TURN CERMET TOP ADJUST 20K RVT26 1 0 BOURNS 3296 1 203 4 901 3600 000 SOCKET IC GOLD PLD DUAL LEAF 8 XA1 1 0 EA 2 640463 4 4 902 3600 000 SOCKET IC GOLD PLO DUAL LEAF 8 PIN 2 1 0 2 640463 4 4 903 3600 000
35. 1 0 EA DALE CCF 55 6 1038 3500 042 RES FILM 0 25 1 100PPM 15K R38 1 0 EA DALE 55 amp 1039 3500 123 RES FILM 0 254 1 100 3328 R39 1 0 EA DALE CCF 55 6 1040 3500 006 RES FILM 0 259 1 100 1K 40 1 0 EA DALE CCF 55 6 1041 3500 039 RES FILM 0 25W 1 100 1 5 41 1 0 DALE 55 6 1042 3500 159 RES FILM 0 25W 1 100 4 75 R42 1 0 DALE CCF 55 4 1043 3500 096 RES FILM MET 0 25W 1 100PPM 2K74 R43 1 0 EA DALE CCF 55 4 104 3500 096 RES FILM MET 0 25W 1 100PPM 2 74 R44 1 0 EA DALE CCF 55 4 1045 3500 002 RES FILM 0 25 1 100 10R R45 1 0 EA DALE 55 4 1046 3500 009 RES FILM MET 0 25W 1 100 10K R46 1 0 EA DALE CCF 55 4 1047 3500 009 RES FILM 0 25W 1 100 10K R47 1 0 DALE CCF 55 6 1058 3500 009 RES FILM 0 254 1 100PPM 10K R48 1 0 EA DALE CCF 55 4 1049 3500 006 RES FILM 0 254 1X 100 1 R49 1 0 EA DALE CCF 55 4 1050 3500 009 RES FILM MET 0 25W 1 100PPM 10K R50 1 0 EA DALE CCF 55 6 1051 3560 001 RES FILM 0 254 0 1 25 10 00 R51 1 0 EA DALE 55 9 4 1052 3560 007 RES FILM 0 254 0 1 25PPM 15 00 R52 1 0 EA DALE 55 9 4 1053 3560 007 RES FILM 0 254 0 1 25 15 00 R53 1 0 EA DALE CMF 55 T9 B 6 1054 3560 001 RES FILM 0 254 0 1
36. 1 3800 002 TERMINAL TEST EYELET TP11 71 0 BICCVERO 20 21370 5 801 3720 019 SWITCH POWER PB MOUNT 2P2T 1 1 0 SCHADOW 51811 5 802 3720 020 SWITCH CAP POWER BLACK 1 1 0 SCHADOW 21275 5 803 3720 018 SWITCH PLUNGER EXTENDER FOR PUSHBUTTON S1 6 0 EA SCHADOW 51985 1 5 1 0 804 3740 005 SWITCH SLIDE MOUNT 2P ON NONE ON 52 202031MS020A 17 usr qad c4 p 2 4 BILL OF MATERIAL ITEM REPORT Date 02 13 90 17 Axon Instruments Inc Time 12 49 59 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 5 901 3430 005 PCB POWER SUPPLY 5 OUTPUT NONE 1 0 EA 5 902 3435 005 01 E SCH POWER SUPPLY AXOCLAMP 3 1 0 EA 3 501 2010 000 B ASSY MATERIAL AXOCLAMP 2 n NONE 1 0 EA y AXON 4 1 5430 000 SCREM PHH PNH CHROME M X 6 NONE 6 0 EA ELMA 1912 06 4 2 5430 002 SCREW PNH SST M3 X5 NONE 6 0 EA BAYCITY 3 5 55 4 4 5410 002 4 SCREW PNH SST 44 40 X 0 375 6 0 BAYCITY 2 2 5 5430 001 SCREW OVAL PLD 100 DEG M3 X 10 NONE 8 0 ELMA 1905 14 2S4 11 5210 006 NUT HEX PLD 0 375 32 X 0 5 X 0 094 NONE 9 0 EA 4 12 5210 010 NUT 0 25 40 0 313 X 0 062 NONE 3 0 EA 7002 4 13 5210 009 NUT DRESS 0 25 40 FOR TOGGLE SWITCHES NONE 6 0 7099 4 14 3250 005 CONN NUT FOR BNC PLD 0 375 24 0 5 X 0 188 NON
37. 100 1K R71 1 0 DALE 55 4 1072 3500 042 RES FILM MET 0 254 1 100 15K R72 1 0 EA DALE CCF 55 4 1073 3500 006 RES FILM 0 25W 1 100PPM 1K R73 1 0 EA DALE CCF 55 4 1074 3500 002 RES FILM 0 25W 1 108 R74 1 0 EA DALE CCF 55 4 1075 3500 123 RES FILM MET 0 25W 1 100 3328 R75 1 0 EA DALE CCF 55 4 1076 3500 006 RES FILM MET 0 254 1 100PPM 1K R76 1 0 DALE CCF 55 4 1077 3500 039 RES FILM MET 0 254 1 100PPM 1 5 R77 1 0 DALE CCF 55 4 1078 3500 002 RES FILM 0 25W 1 100PPM 108 R78 1 0 EA DALE CCF 55 4 1079 3500 081 RES FILM 0 250 1 100 2 2K21 R79 1 0 DALE CCF 55 4 1080 3500 189 RES FILM 0 25W 1 100 5K62 R80 1 0 DALE CCF 55 amp 1081 3500 009 RES FILM MET 0 25W 100PPM 10K R81 1 0 EA DALE 55 4 1082 3500 009 RES FILM 0 25W 1 100PPM 10K R82 1 0 DALE 55 4 1083 3500 009 RES FILM 0 25W 1 100PPM 10K R83 1 0 EA DALE 55 4 1084 3560 001 RES FILM 0 25W 0 1 25 10 00 R84 1 0 EA DALE CMF 55 T9 B 4 1085 3560 007 RES FILM MET 0 254 0 1 25PPM 15 00 R85 1 0 EA DALE CMF 55 T9 B 4 1086 3560 007 RES FILM MET 0 250 0 1 25PPM 15 00 R86 1 0 EA DALE CMF 55 T9 B C 8 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 8 Axon Instruments Inc Time 12 48 24 Le
38. 1002 This section is similar to the ME2 section with the following differences The CCS A6 is connected to the command summing amplifier A9 in all modes A9 has a fixed gain During two electrode clamp relay RL2 bypasses the CCS and connects the output of the 30 V amplifier 7 to Ro There is no lowpass output filter AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 4 Switches 53 5 determine position of the decimal point in the current meter One of these three switches is activated depending on the position of the Current Display Select switch 52 VOLTAGE CLAMP AND V1 CONTINUOUS ASSEMBLY No A1002 Voltage Clamp The microelectrode headstage voltage is amplified 20 times in amplifier A21 The bath potential is lowpass filtered by A29 and subtracted from V1A in A21 If no bath headstage is used R218 sets OV i The output of A21 goes through the lowpass Anti Aliasing filter which is useful in DCC and dSEVC modes and becomes the input of A22 a high speed unity gain sample and hold amplifier The output of A22 20 is used the MEI section to form the 10 output 20 also becomes the input of the summing amplifier A24 A24 20V is compared with the voltage clamp command voltages coming out of A27 The phase shift network adds phase lag or lead to the output of A24 The signal is then amplified in A25 and A26 to produce the v
39. 100K 4758 1508 6K81 6K81 Qty UM P Mfg 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA CORNING 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA OALE 1 0 DALE 1 0 DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA 1 0 EA DALE 1 0 DALE 1 0 EA DALE 1 0 DALE 1 0 EA DALE 1 0 EA 1 0 DALE 1 0 DALE 1 0 DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA ALLNBRAD 1 0 EA DALE 1 0 DALE 1 0 DALE 1 0 DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA DALE 1 0 DALE 1 0 EA DALE 1 0 EA DALE 1 0 EA CORNING 1 0 DALE 1 0 DALE 1 0 EA DALE Date 02 13 90 Time 12 48 45 Manufacturer Part CMF 55 T9 B 55 55 55 55 55 CCF 55 RN60DA750F CCF 55 55 55 55 55 55 55 55 55 CCF 55 CCF 55 55 55 55 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 CCF 55 55 CCF 55 CCF 55 RCO7GF106J CCF 55 CMF 55 T1 CCF 55 CCF 55 CCF 55 55 CCF 55 55 CCF 55 CCF 55 RN60D4750F CCF 55 CCF 55 CCF 55 11 usr qad c4 p 2 4 BILL OF MATERIAL BY REPORT Date 02 13 90 11 Axon Instruments Inc Time 12 48 51 Level Item Component Rev D
40. 1120 3500 159 RES FILM MET 0 25W 1 100PPM 4K75 R120 1 0 EA DALE CCF 55 4 1121 3500 162 RES FILM 0 25W 1 100 47 5 R121 1 0 EA DALE CCF 55 4 1122 3500 159 RES FILM MET 0 254 1 100PPM 4K75 R122 1 0 EA DALE CCF 55 6 1123 3500 162 RES FILM 0 25W 1 100 47 5 R123 1 0 EA DALE 55 4 1124 3500 159 RES FILM 0 25W 1 100 4K75 R124 1 0 EA DALE 55 amp 1125 3500 162 RES FILM 0 254 1 47 5 R125 1 0 EA DALE CCF 55 4 1126 3500 159 RES FILM 0 254 1 100PPM 4K75 126 1 0 DALE CCF 55 4 1127 3500 162 RES FILM 0 25W 1 100PPM 47 5 R127 1 0 DALE CCF 55 4 1128 3500 204 RES FILM MET 0 25W 1 6 81 R128 1 0 EA DALE CCF 55 4 1129 3500 204 RES FILM MET 0 25W 1 100PPM 6 81 R129 1 0 EA DALE CCF 55 4 1130 3500 204 RES FILM MET 0 254 1X 100PPM 6K81 R130 1 0 EA DALE 55 4 1131 3500 204 RES FILM 0 254 1 100PPM 6K81 R131 1 0 EA DALE CCF 55 4 1132 3500 204 RES FILM 0 254 1 100PPM 6K81 R132 1 0 DALE CCF 55 amp 1133 3500 204 gt RES FILM MET 0 25W 1 100PPM 6K81 R133 1 0 DALE CCF 55 6 1134 3500 204 RES FILM 0 25W 1 100 6K81 R134 1 0 EA DALE CCF 55 4 1135 3500 075 RES FILM 0 25W 1 100 2281 R135 1 0 DALE 55 4 1136 3500 075 RES FILM 0 25W 1 100
41. 15 _ RES FILM 0 254 1X 100PPM 1M R5 1 0 EA DALE CCF 55 amp 4 550 3400 021 SGL TURN PLSTC PC MOUNT LOG 500K RTI 1 0 CLARSTAT 31664AJ5003 4 601 3720 000 SWITCH MINI 1P2T 1 1 0 81215726 amp 602 3720 000 SWITCH PB MINI MOM 1P2T 52 1 0 EA 8121SYZGE 6 801 3430 002 D PCB AXOCLAMP REMOTE BUZZ 059 1 0 EA 18 usr qad c4 p 18 Level Item Component 2 4 BILL OF MATERIAL BY ITEM REPORT Axon Instruments Inc Description Qty UM P Mfg Date 02 13 90 Time 12 50 10 Manufacturer Part 3 101 101 201 3 201 4 101 4 102 4 103 4 201 301 302 303 401 501 605 o 8 UI HE Ui 4 3435 002 2100 048 3010 003 5020 003 3200 001 3200 003 5020 006 3720 002 3720 003 3310 033 3310 018 2600 096 5300 047 5305 047 5440 007 6600 001 2050 007 3720 006 6600 000 2950 033 2300 020 2100 045 3235 004 3235 004 3900 031 5600 001 2600 016 5200 010 5200 010 3230 009 5010 002 3530 008 3530 008 3530 008 3100 010 3750 018 3230 009 2600 067 5300 001 5305 001 5440 007 6600 001 2200 015 3010 011 3010 013 3010 014 3010 015 2300 055 3710 000 3235 006 3235 005 3010 001 gt Om gt gt gt gt gt SCH AXOCLAMP REMOTE BUZZ
42. 2 5 22 1002152 5 4420 0 HIX6 1 8 09 9101 1 03 E10970 CS005 50M 2 CS005 50M 2X CS005 50M 2X EPT3X4 470PF Y5P10 1015 2 09 9101 1 03 2514 6X8Z1P2MIL 17742 86511050 86516035 86552005 491 108 1082 001 108 0312 001 19122 usr qad c4 p gt NN NN STRAIN RELIEF 0 245 CABLE 5MN 3 PACKAGING AXOCLAMP PACKAGING RSC 275 D W 21 75 X 18 X 13 75 PACKAGING MAILER 200 LB 12 11 2 MANUALS PACKAGING FOAM BLOCKS SET OF 4 CLAMP S U PUSH ON C ABLE AI 2005 1 2010 CABLE BNC PUSH ON 150MM CABLE BNC PUSH ON 1M PACKAGING ZIP LOCK BAG 6 X 8 X 0 002 LABEL PROMOTIONAL SAMPLE PUSH ON CABLE LABEL 1 0 X 0 5 WHITE POLYESTER LETTER PROMOTIONAL SAMPLE PUSH ON CABLE 5020 005 2900 010 6610 000 6610 001 6620 000 2950 108 2100 000 2100 003 6600 001 2400 066 6300 010 9320 032 2 4 BILL OF MATERIAL BY ITEM REPORT Axon Instruments Inc gt wa oooooooo m m gt d gt ad eb gt End of Report 19 Date 02 13 90 12 50 27 Manufacturer Part 00125 PUSH ON CABLE SET 1103 27 6 1103 27 40 6 821 2 11 DAT 20 619 DR HARVEY J UNIVERSITY OF CALIFORNIA SAN DIEGO DEPARTMENT OF NEUROSCIENCES 0608 9500 GILMAN DRIVE LA JOLLA CA 92093 0608 CHANGES THE SERVICE MANUAL To keep abreast of newly developed electronic components technologies and user comm
43. 200 022 45V 464 2 22645 5 1 0 EA DATEL AM464 2 4 6 4200 009 IC OP AMP FET LT1056ACH 1 0 EA LINTECH LT1056ACH 2 4 7 4200 022 IC 45V 464 2 22645 1 0 DATEL 4 64 2 4 8 4200 008 1 111056 j A8 1 0 LINTECH LT1056CN8 2409 4200 000 IC LOW OFFSET 171001 9 1 0 EA LINTECH LT1001CN8 4 10 4200 016 1 OP QUAD MC34004 10 1 0 LINTECH LT1058CN8 24 1 4200 001 CP AMP LOW NOISE 171007 11 1 0 LINTECH LT1007CN8 SA 12 4200 022 45 464 2 22645 12 1 0 EA DATEL 464 2 4 13 4200 001 1 AMP LOW NOISE 111007 15 1 0 EA LINTECH LT1007CN8 2 4 14 4200 008 IC OP AMP FET LT1056 14 1 0 LINTECH 111056648 4 015 4200 009 IC OP AMP FET LT1056ACH 15 1 0 LINTECH LT1056ACH amp 16 4200 008 1C OP AMP FET LT1056 A16 1 0 LINTECH LT1056CN8 6 17 4450 000 IC S 5320 5 SHM 20C A17 1 0 EA DATELINT SHM 20C 2 77 200 016 IC OP ASP QUAD MC34004 18 1 0 LINTECH LT1058cN8 4 19 4200 016 10 OP AMP QUAD MC3S4004 19 1 0 EA LINTECH LT1058CN8 4 20 4200 016 IC OP QUAD MC34004 20 1 0 EA LINTECH LT1058CN8 4 21 4200 020 1 LF357 i A21 1 0 MOTOROLA LF357J 6 22 4450 000 IC S 5320 5 20 A22 1 0 DATELINT SHM 20C e 23 4200 017 IC OP AMP DUAL LF353 A23 1 0 EA MOTOROLA LF353
44. 35V 1U 4 C38 1 0 EA KEMET 1353 105 035 5 amp 439 3130 000 CAP FILM 10 100v 0 0010 c39 1 0 ARCO PRI 102 K 2A amp 440 3100 020 CAP CER ML 50V 0 21 0 10 C40 1 0 KEMET 323 1042505 924 4 441 3100 020 CAP CER ML 50V 0 21N 0 1U 641 1 0 323 1042505 924 4 442 3100 020 CER ML 50V 0 216 0 10 642 1 0 C323C10425U5CAC924 amp 443 3100 020 CAP CER ML 50V 0 2IN 0 1U 1 0 KEMET 323 1042505 924 4 444 3100 020 CER ML 50V 0 2IN 0 10 Chh 1 0 C323C10425U5CAC924 amp 645 3100 020 CAP CER ML 50V 0 21 0 1U C45 1 0 KEMET 325 10425 5 924 4 446 3100 020 CER ML 50V 0 21 0 1U C46 1 0 323 1042505 924 4 447 3100 020 CAP CER ML 50V O 2IN 0 10 C47 1 0 EA 323 1042505 924 4 448 3100 020 CAP CER ML 50V 0 21 0 10 648 1 0 323 1042505 924 4 449 3100 020 CAP CER ML 50V 0 21 0 1U C49 1 0 KEMET C323C10425USCAC924 4 450 3100 020 CAP CER ML 50V 0 21 0 10 C50 1 0 KEMET 323 1042505 924 4 451 3100 020 CAP CER ML 50V 0 21N 0 10 C51 1 0 EA KEMET C323C10425U5CACO24 4 452 3100 020 CAP CER ML 50V 0 21 0 1U 652 1 0 323 1042505 924 4 453 3100 020 CAP CER ML 50V 0 21N 0 10 653 1 0 632361042515 924 6 45
45. 4 3100 020 gt CER ML 50V 0 211 0 10 C54 1 0 KEMET C323C10425U5CAC924 4 455 3100 020 CAP CER ML 50V 0 216 0 10 655 1 0 KEMET 323 1042505 924 4 456 3100 020 CAP ML 50V 0 218 0 10 C56 1 0 EA C323C10425U5CAC924 4 457 3100 020 CAP CER ML 50V 0 21 0 10 57 1 0 323 10425 5 924 4 458 3100 020 CER ML 50V 0 214 0 10 58 1 0 C323C10425U5CAC924 4 459 3100 020 CER ML 50V 0 21N 0 10 C59 1 0 EA KEMET C323C10425U5CAC924 4 460 3100 020 CAP CER ML 50V 0 214 0 10 C60 1 0 EA 323 1042505 924 amp 461 3100 020 CAP CER ML 50V 0 21N 0 1U c61 1 0 323010425 5 920 4 462 3100 020 CAP CER ML 50V 0 21 0 10 C62 1 0 KEMET 323 1042505 924 amp 463 3100 020 CAP CER ML 50V 0 21N 0 10 C63 1 0 EA 323 1042505 924 4 464 3100 020 CAP CER ML 50V 0 21N 0 1U C64 1 0 EA 323 1042505 924 4 465 3100 020 CAP CER ML 50V 0 21 0 10 C65 1 0 EA KEMET C323C10425U5CAC926 amp 466 3100 020 CAP CER ML 50V 0 21N 0 10 C66 1 0 EA 323 1042505 924 4 467 3160 000 CAP TANT 35V 1U 67 1 0 KEMET T353A105K035AS amp 468 3130 001 CAP FILM 10 100V 0 010 c68 1 0 ARCO PRI 103 K 2A amp 469 3160 000 CAP TANT 35V 10 C69 1 0 EA KEMET T353A105K035AS 4 470 3100 0
46. 4 D 5 205 3220 001 CONN 0 100 STRAIGHT GOLD 8 43 1 0 EA PANDUIT 55100 8 0 5 006 3220 009 CONN INS DISPL SOCKET 0 100 GOLD 8 PIN 1 0 PANDUIT 100 24 8 0 5 207 3220 017 CONN COVER FOR 0 100 INS DISPL 8 P3 1 0 PANDUIT 65 100 80 5 208 3220 010 CONN INS DISPL SOCKET 0 100 GOLD 12 P4 1 0 PANDUIT 100 24 12 5 209 3220 018 CONN COVER FOR 0 100 INS DISPL 12 PIN P4 1 0 EA PANDUIT SCC100F 12T 5 210 3000 001 COMPONENT NOT PRESENT 45 1 0 EA 5 301 4020 001 DIODE SI 1A 400PIV 1N4004 01 1 0 EA 194004 5 302 4020 001 DIODE SI 1 400 1 104004 02 1 0 EA 144004 5 303 4020 001 DIODE SI 1A 400 1 104004 D3 1 0 GENELECT 1N4004 5 401 4020 004 DIODE BRIDGE 1 5 400P IV WO4M BR1 1 0 GENINST WO4M 5 402 4020 004 DIODE BRIDGE 1 5A 400P IV WO4M BR2 1 0 GENINST 04 5 403 4020 004 DIODE BRIDGE 1 5 400P IV WO4M BR3 1 0 EA GENINST 04 5 501 3330 000 HEATSINK PCB MOUNT TO 220 2 IN H1 1 0 EA THRMLLY 60928 5 502 3330 000 HEATSINK MOUNT TO 220 2 IN 1 0 THRMLLY 60928 5 503 3330 000 HEATSINK PCB MOUNT 10 220 2 IN H3 1 0 THRMLLY 60928 5 504 3330 000 HEATSINK PCB MOUNT TO 220 2 IN 4 1 0 EA THRMLLY 60928 5 505 3330 000 HEATSINK PCB MOUNT TO 220 2 IN H5 1 0 EA THRMLLY 60928 5 506 5410 000 SCREW PNH SST
47. 99 4 INSTRUMENTS INC PARON 04 11 89 3430 001 REMOTE AND CONTROL LOGIC peur OOOO Sor 10 LI V J10 1 R239 475 POWER SUPPLY MAIN BOARD 0004 PCB0001 POWER DISTRIBUTION 06 08 90 18 21 DPM DISPLAY REVISION RECORD REV DESCRIPTION OF CHANGE DATE APPROVED 1 mH TP20 3417 19 53 J18 CLOCK 5 4 SAMPLE UNKUP CONNECTOR lus J18 1 TO COUNTER CKT 29 1 26 78 11 5V gt gt 5 8 12 EEENNEILLLII IIIA ATURE OR PARTS UST om juan 0502 pg INSTRUMENTS INC MARC PARON O4 11 89 a CLOCK 2270 024 _______5430 00 AND CONTROL LOGIC UMLESS OTHERWSE 3435 0011 seme emer 1 19 2 8 GND SHIELD 13 GRN Ji 8 SHIELD GND 15V TO 15V TO CONNECT TO 15V TO HEADSTAGE GROUND 5 15 TO AMPS HEADSTAGE MODEL CELL CONFIGURATION SCH OO1L dwg J1 2 518 NOTE 51 52 LOCATED ON 0004 Plotted 04 19 90 13 13 Le couponents LOCATED ON POWER SUPPLY BOARD 0004 REMSION RECORD OF crane
48. 9R2 R6 1 0 DALE CCF 55 4 1007 3500 006 RES FILM 0 25W 1 100PPM 1K R7 1 0 DALE CCF 55 amp 1008 3500 123 RES FILM 0 25W 1X 100PPM 332R R8 1 0 EA DALE 55 4 1009 3500 132 RES FILM MET 0 25W 1 100PPM 332K R9 1 0 EA DALE CCF 55 6 1010 3500 003 RES FILM 0 254 1 100PPM 1008 R10 1 0 EA 55 amp 1011 3500 078 RES FILM 0 259 1 100 221R R11 1 0 DALE CCF 55 amp 1012 3500 171 RES FILM 0 25W 1 4998 R12 1 0 EA DALE CCF 55 4 1013 3500 012 RES FILM 0 25W 1 100PPM 100K R13 1 0 EA DALE 55 4 1014 3560 001 RES FILM 0 25W 0 1 25 10 00 R14 1 0 EA DALE CMF 55 T9 B amp 1015 3500 159 RES FILM MET 0 254 1 100PPM 4K75 R15 1 0 EA CCF 55 4 1016 3500 006 RES FILM MET 0 25W 1 100PPM 1K R16 1 0 DALE CCF 55 4 1017 3500 231 RES FILM MET 0 25W 1 100PPM 9K R17 1 0 DALE CMF 55 T1 4 1018 3500 015 RES FILM 0 254 1 100PPM 1M R18 1 0 EA DALE CCF 55 amp 1019 3560 001 RES FILM MET 0 25W 0 1 25 10 00 R19 1 0 EA DALE CMF 55 T9 B 6 1020 3560 001 RES FILM MET 0 25W 0 1 25 10 00 R20 1 0 EA DALE CMF 55 T9 B amp 1021 3500 009 RES FILM 0 25W 1X 100PPM 10K R21 1 0 DALE CCF 55 4 1022 3500 180 RES FILM MET 0 25W 1 100PPM 499K R22 1 0 EA DALE CCF 55 4 1023 3560 001 RES FILM 0 25W
49. CLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 3 5 REFERENCE ST COMMAND GENERATOR DESTINATION SWITCH ASSEMBLY No 1002 Voltage reference 2 and inverting amplifier provide a stable 5V and 5V reference for use by the rest of the circuit 1 and A2 located on the thumbwheel switch board comprise a multiplying D A converter of A2 corresponds to the setting of the switch Polarity is changed by swapping the D A input between Vg and Vg U12 is a CMOS switch used to convert the D A output into a ground referenced voltage step Duration of the step is set by the External Step Activate input or the front panel switch Destination switch determines whether the step voltage will be passed to the section the ME2 section or the voltage clamp section calibration signal is generated in A1 and is proportional to the setting of the thumbwheel switch Duration of the calibration pulse is set by the external Cal Activate input MICROELECTRODE 1 ASSEMBLY 1002 Transistors Q3 and Q4 and current regulator diodes CRD3 and CRD4 comprise a floating 10 V power supply for the headstages transistors are configured to simulate 10 V zeners common point of the floating supply is driven by the electrode voltage V1A 12 is a 30 V amplifier providing positive feedback to the headstage input through
50. Cy inside the headstage This feedback compensates the input capacitance capacitance neutralization 15 and inside the headstage comprise a constant current source CCS command voltage on R53 is forced across Ro thereby setting the electrode current The command voltage either comes from the voltage clamp circuit or from summing amplifier A16 depending on the position of relay RL6 The gain of A16 is boosted 3 3 times by RL6 during DCC mode This 3 3 boost compensates for the 3096 current duty cycle during discontinuous current passing Relays RL8 9 set the command voltage of the CCS to large positive or negative values resulting large currents are used to clear blocked electrodes The Bridge pot A28 and A32 are used to subtract the voltage drop across the electrode from the total electrode voltage This voltage drop is the product of the measured current and the Bridge pot setting DCC mode the Bridge pot is disabled by RL10 cSEVC mode the subtraction range is reduced to one tenth 19 buffers a first order lowpass filter Electrode current is proportional to the voltage drop across Ro This is measured by A14 Sample and hold A17 acts as a unity gain buffer during Bridge mode During DCC it samples the current pulses RL11 changes the current scaling during DCC to compensate the measurement for the 30 duty cycle A18 buffers a first order lowpass filter MICROELECTRODE 2 ME2 ASSEMBLY No A
51. DEG W BRKT 15 46 1 0 117 DA 15SA 4 607 3200 005 CONN D SUB FEMALE 90 DEG W BRKT 15 PIN 47 1 0 EA AMP 117 DA 15SA 4 608 3220 002 CONN PCB 0 100 STRAIGHT GOLD 12 PIN J8 1 0 EA PANDUIT 55100 12 4 609 3220 001 CONN PCB 0 100 STRAIGHT GOLD 8 PIN 39 1 0 EA PANDUIT MPSS100 8 D A amp 610 3220 003 CONN PCB 0 100 STRAIGHT GOLD 16 PIN 410 1 0 EA PANDUIT 55100 16 4 611 3220 002 CONN PCB 0 100 STRAIGHT GOLD 12 PIN J11 1 0 PANDUIT 55100 12 4 612 3220 004 CONN PCB 0 100 STRAIGHT GOLD 20 PIN J12 1 0 EA PANDUIT 55100 20 4 613 3220 004 CONN PCB 0 100 STRAIGHT GOLD 20 PIN J13 1 0 EA PANDUIT MPSS100 20 C A amp 614 3220 003 CONN PCB 0 100 STRAIGHT GOLD 16 PIN 414 1 0 EA PANDUIT MPSS100 16 T A 4 615 3220 001 CONN PCB 0 100 STRAIGHT GOLD 8 PIN J15 1 0 EA PANDUIT MPSS100 8 D A 4 616 3220 000 CONN PCB 0 100 STRAIGHT GOLD 4 PIN J16 1 0 EA PANDUIT MPSS100 4 D A 4 617 3220 000 CONN PCB 0 100 STRAIGHT GOLD 4 PIN J17 1 0 EA PANDUIT MPSS100 4 D A 4 618 3220 002 CONN PCB 0 100 STRAIGHT GOLD 12 PIN J18 1 0 PANDUIT MPSS100 12 T A 4 619 3220 000 CONN PCB 0 100 STRAIGHT GOLD 4 PIN 419 1 0 EA PANDUIT MPSS100 4 D A 4 701 3330 003 HEATSINK TO 5 PRESS AS 1 0 THRMLLY 22258 4 702 3330 003 HEATSINK TO 5 PRESS 1 0 THRMLLY 22258 4 705 3330 003 HEATSINK TO 5 PRESS Al2 1 0
52. DR HARVEY J KARTEN UNIVERSITY OF CALIFORNIA SAN DIEGO DEPARTMENT OF NEUROSCIENCES 0608 February 1990 9500 GILMAN DRIVE 92093 0608 AXOCLAMP 2A MICROELECTRODE CLAMP SERVICE PLEASE CHECK FOR CHANGE INFORMATION AT THE REAR OF THIS MANUAL Copyright 1987 1990 Axon Instruments Inc No part of this manual may be reproduced stored in a retrieval system or transmitted in any form or by any means electronic mechanical photocopying microfilming recording or otherwise without written permission from Axon Instruments Inc QUESTIONS Call 415 571 9400 Part Number 2500 001 REV A Printed in U S A iii COPYRIGHT THE CIRCUITS AND INFORMATION IN THIS MANUAL ARE COPYRIGHTED AND MUST NOT BE REPRODUCED IN ANY FORM WHATSOEVER WITHOUT WRITTEN PERMISSION FROM AXON INSTRUMENTS INC WARNING THIS INSTRUMENT OPERATES FROM LINE VOLTAGES DO NOT PERFORM ANY SERVICING UNLESS YOU ARE QUALIFIED TO SO LINE VOLTAGES ARE PRESENT IN THE LEFT MOST SECTION BETWEEN THE POWER ON OFF SWITCH AND THE LINE INPUT FILTER DR HARVEY J KARTEN M D UNIVERSITY OF CALIFORNIA SAN DIEGO DEPARTMENT OF NEUROSCIENCES 0608 9500 GILMAN DRIVE 92093 0608 AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC iv This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC TABLE CONTENTS Page THEORY OF OPERA TION
53. DVM digital volt meter DPM digital panel meter EQUIPMENT REQUIRED Differential input scope with 50 V div resolution DVM Square wave generator Frequency meter TRIM PROCEDURE It is important to do the trims in the order presented because some of them rely on earlier trims For brevity only changes to the previous setup are listed for each trim MEASUREMENT TECHNIQUES Measuring 0 V You will be asked to trim for 0 V differentially e g across TP8 First connect both probes from the scope to the ground point the second mentioned test point in this example TP8 to establish 0 V Next connect the probes separately to the two test points and make the requested trim Filtering When measuring zero volts or establishing a null the input to the scope should be lowpass filtered at 10 kHz or less Measuring DC voltages eliminate offset errors from measurement all of these measurements will be balanced That is the command potential will be switched alternately from ve to ve and the difference in the measured outputs will be measured Connect DVM ground to yellow ground plug at rear of unit H Some measurements require x1 headstage and 10 1 resistor If you do not have headstage a x0 1 headstage can be used with a 100 MQ 1 resistor 1 Counter Connect frequency meter to TP20 Set Rate pot for about 500 Hz on frequency meter Trim RVT21 for same reading on panel counter Set Rate po
54. E 6 0 BAYCITY 4 21 5700 004 WASHER LOCK INTL PLD 0 375 10 0 500 00 NONE 9 0 EA BAYCITY 4 22 5700 003 WASHER LOCK INTL PLD 0 25 ID X 0 4 OD NONE 3 0 EA 7003 3 amp 23 5700 001 i WASHER LOCK INTL PLD 4 X 0 26 00 NONE 27 0 EA BAYCITY 6 41 5700 005 WASHER RETAINING FOR 0 25 KEYWAY BUSHING NONE 8 0 7007 2 2 601 2600 051 MECH COVER 420 305 MM COMPLETE NONE 2 0 EA AXON CLAMP COVER mu 1 5100 007 CHASSIS TOP BOTTOM COVER 420 X 305 MM NONE 1 0 ELMA 1466 83 443 701 6290 011 DOC LINE VOLTAGE INFORMATION NONE 1 0 EA 3 801 6600 003 PACKAGING POLYBAG 24 X 38 X 0 002 NONE 1 0 EA BARKOFF 24X38 POLY 1 102 2500 001 A 7 MANUAL SERVICE AXOCLAMP 2 NONE 1 0 EA AXON e 1 6020 002 DOC COVER SET W WINDOW NAV BLU PRINTED NONE 1 0 EA GBC 5000052 2 2 6020 004 BINDING SPIRAL 0 375 DIAMETER WITH NONE 1 0 EA AXON 2 3 3435 001 K SCH AXOCLAMP 2 MAIN 0 0 e 6 3435 002 D SCH AXOCLAMP REMOTE BUZZ 0 0 lt 2 5 3435 004 SCH THUMBWHEEL TO VOLTAGE CONVERTER 0 0 EA 2 6 3435 005 01 E SCH POWER SUPPLY AXOCLAMP 0 0 EA AXON 1 103 6290 008 A DOC PRINTED WARRANTY REGISTRATION NONE 1 0 EA AXON 1 104 6290 004 C DOC PROBLEMS AND SUGGESTIONS REPORT NONE 2 0 EA 1 105 6250 011 DOC SHIP CHECK LIST AXOCLAMP NONE 1 0 EA 1 201 2950 035 S U REMOTE BUZZ AXOCLAMP
55. EL MOUNT FEMALE CONN BNC ISOLATED PANEL MOUNT FEMALE CONN BNC ISOLATED PANEL MOUNT FEMALE CONN BNC ISOLATED PANEL MOUNT FEMALE CONN BNC ISOLATED PANEL MOUNT FEMALE CONN BNC ISOLATED PANEL MOUNT FEMALE CONN BNC ISOLATED PANEL MOUNT FEMALE JACK BANANA 4 44MM PANEL YELLOW JACK BANANA 4 44MM PANEL VIOLET JACK BANANA 4 44MM PANEL VIOLET JACK BANANA 4 44MM PANEL RED CONN D SUB FEMALE SOLDER CUP 15 PIN CONN LINE W 1A FILTER W 5X20MM FUSE HOLDER FUSE 5 X 20MM SLOW 500 FUSE 5 X 20MM SLOW 500MA CONN INS DISPL SOCKET 0 100 GOLD 8 PIN CONN INS DISPL SOCKET 0 100 GOLD 12 PIN CONN INS DISPL SOCKET 0 100 GOLD 4 PIN CONN INS DISPL SOCKET 0 100 GOLD 12 PIN CONN INS DISPL SOCKET 0 100 GOLD 4 PIN CONN COVER FOR 0 100 INS DISPL 8 PIN CONN COVER FOR 0 100 INS DISPL 12 PIN CONN COVER FOR 0 100 INS DISPL 4 PIN CONN COVER FOR 0 100 INS DISPL 12 PIN CONN COVER FOR 0 100 INS DISPL 4 PIN MECH PANEL REAR AXOCLAMP 2 PANEL REAR AXOCLAMP 2 PANEL ARTWORK REAR AXOCLAMP 2 ELMCH CLAMP CHASSIS POWER SUPPLY CHASSIS LONG SECT 420MM CHASSIS DEPTH REDUCING SECT 420 MM CHASSIS SIDE MALLS WITH FEET PAIR 4E SPACER 0 75 X 0 25 4 40 SPACER ALUM 0 625 X 0 25 84 40 TRANSFORMER LOW HUM 5 OUTPUT TERMINAL SOLDER LUG 4 MOUNT 1 CHASSIS PANEL BASE FOR POWER SUPPLY CHASSIS PANEL VERTICAL SHIELD BUSHING NYLON 0 875 OD X 0
56. F 14 PIN XU3 1 0 EA 2 640357 4 4 954 3600 004 SOCKET 1 GOLD PLD DUAL LEAF 14 PIN XU4 1 0 EA AMP 2 640357 4 amp 955 3600 005 SOCKET GOLD PLD DUAL LEAF 16 PIN 5 1 0 2 640358 4 6 956 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN 1 0 2 640357 4 amp 957 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN 1 0 2 640357 4 6 958 3600 005 SOCKET IC GOLD PLD DUAL LEAF 16 PIN 1 0 2 640358 4 amp 959 3600 005 SOCKET IC GOLD PLD DUAL LEAF 16 PIN 9 1 0 2 640358 4 6 960 3600 005 SOCKET IC GOLD PLD DUAL LEAF 16 1 1 0 2 640358 4 6 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT 3 Date 02 13 90 6 Axon Instruments Inc Time 12 48 09 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 4 961 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN 11 1 0 2 640357 4 4 962 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 Xu12 1 0 EA 2 640357 4 amp 963 3600 005 SOCKET IC GOLD PLD DUAL LEAF 16 PIN XU13 1 0 EA 2 640358 4 amp 964 3000 001 COMPONENT NOT PRESENT Xu14 1 0 EA 4 965 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XU15 1 0 AMP 2 640357 4 6 981 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL1 1 0 2 640357 4 4 982 3600 004 SOCKET IC GOLD PLD DUAL LEAF 14 PIN XRL2 1 0
57. H mode or through the cell CELL mode Special plugs connect directly to the headstages GROUNDING Signal ground is isolated from the chassis and power ground CONTROL INPUTS Above 3 V is accepted as logic HIGH Below 2 V is accepted as logic LOW Inputs are protected to 15 HEADSTAGE DIMENSIONS Case is 2 25 x 1 14 x 0 87 57 2 x 29 0 x 22 1 mm Mounting rod is 4 102 mm long Available mounting rod diameters are 1 4 5 16 or 3 8 6 3 7 9 or 9 5 mm Specify required mounting rod diameter with order Input sockets for the microelectrode shield and ground are 0 08 2 mm diameter Cable length is 10 feet 3 m AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 14 SPECIFICATIONS CASE DIMENSIONS 7 177 mm high 19 483 mm wide 12 5 317 mm deep Mounts in standard 19 rack Handles are included Net weight 18 16 8 kgs B SUPPLY REQUIREMENTS Line voltage 100 125 Vac 200 250 Vac User selectable by an internal switch Line Frequency 50 60 Hz 20 Fuse 0 5 A slow 5 20 mm Line Filter RFI filter is included Line Cord Shielded line cord is provided ACCESSORIES PROVIDED Operator s amp Service Manuals 2 mm plugs for use with headstages Low capacitance test resistor for each headstage Spare globes for Mode switches Spare fuse Footswitches to operate Buzz of both electrodes Clamp 1 Model Cell Remote Buzz Duration hand h
58. HT FEBRUARY 1990 AXON INSTRUMENTS INC Appendix 3 8 1 COM offset Thumbwheel 000 0 external DCC mode minimum cycling rate On scope measure 2 1 TP8 gnd at 50 u V div Trim 13 for no step during the cycle 9 CCSI offset Bridge mode Trim for 0 V 10 Im Bridge offset On scope measure TP11 Im TP8 gnd at 100 u V div Trim RVT9 for 0 V 11 Im DCC offset DCC mode 10 kHz Set Vm Im output bandwidth to 0 1 kHz Trim RVT14 for 0 V J Repeat 9 and 10 12 Headstage offset Bridge mode On scope measure TP1 V 1a TP8 gnd at 100 pV div Plug headstage into slot Ground via resistor of value 10 MQ H Wait 5 min after plugging in headstage Adjust trim pot inside headstage till there is no shift when the grounding resistor is replaced by a resistor of value 1 MQ H Repeat for other headstages 13 Bridge Balance accuracy scope measure TP13 10 Vm TP8 gnd at 20 mV div Plug in x1 headstage Ground via 10 MQ 1 or better Set Bridge Balance to indicate 10 MQ Externally activate thumbwheel at 100 Hz Thumbwheel on 050 0 J Trim RVT23 for a null 14 MEI Bridge offset Switch step command off Use Offset control to center trace on screen Trim 26 so that trace does not shift as Bridge pot increased to maximum AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 4 Appendix 15 10
59. K R184 1 0 EA DALE CCF 55 amp 1185 3500 219 RES FILM MET 0 254 1 100PPM 8K25 R185 1 0 EA DALE 55 2 8 1186 3500 012 gt RES FILM 0 254 1 100 100K R186 1 0 DALE CCF 55 4 1187 3500 009 RES FILM MET 0 25 1X 100PPM 10K R187 1 0 EA DALE CCF 55 amp 1188 3500 012 RES FILM 0 25 1 100PPM 100K R188 1 0 EA DALE CCF 55 4 1189 3500 156 RES FILM 0 254 1 100 475R R189 1 0 EA CORNING RN60D4750F 2 6 1 0 DALE CMF 55 T9 B 1190 3560 001 RES FILM MET 0 254 0 1 25 10K00 R190 10 usr qad c4 p Page 10 MODE TU eiie R Wa 0 e b dad x o We Ro CR V m UL VET MT OE V DEC LR Dae JR EA 3560 001 3500 171 3500 141 3500 159 3500 057 3500 066 3500 078 3500 156 3500 006 3500 057 3500 078 3500 003 3500 012 3500 069 3500 093 3500 078 3500 177 3500 009 3500 171 3500 012 3500 009 3500 204 3500 009 3500 003 3500 183 3500 021 3500 159 3500 159 3500 126 3500 213 3500 057 3500 081 3500 081 3500 141 3500 075 3500 075 3500 171 3530 001 3500 159 3500 234 3500 009 3500 027 3500 081 3500 159 3500 012 3500 003 3500 162 3500 012 3500 156 3500 036 3500 204 3500 204 Description HI MEG FILM FILM FILM FILM FILM FILM
60. LE 55 4 1168 3500 009 RES FILM MET 0 25W 1 100 10K R168 1 0 EA DALE 55 4 1169 3500 009 RES FILM 0 254 1X 100PPM 10K R169 1 0 EA DALE CCF 55 4 1170 3500 126 RES FILM 0 254 1 100PPM 3K32 R170 1 0 EA DALE CCF 55 4 1171 3500 054 RES FILM MET 0 254 1 100 1 82 R171 1 0 EA DALE CCF 55 4 1172 3500 054 RES FILM 0 254 1 100 1 82 R172 1 0 EA DALE CCF 55 4 1173 3500 147 RES FILM MET 0 25W 1 100PPM 39 2 R173 1 0 DALE 55 4 1174 3500 147 RES FILM 0 254 1 100PPM 39K2 174 1 0 DALE 55 6 1175 3500 186 RES FILM 0 250 1 562R R175 1 0 EA DALE 55 4 1176 3500 051 RES FILM 0 254 1 100PPM 182R 176 1 0 EA DALE CCF 55 4 1177 3500 024 RES FILM MET 0 25W 1X 100PPM 1 21 R177 1 0 EA DALE 55 4 1178 3500 024 RES FILM MET 0 25W 1 100PPM 1K21 _ R178 1 0 EA DALE 55 4 1179 3500 015 RES FILM 0 254 1 100 1M R179 1 0 DALE 7 55 4 1180 3500 002 RES FILM MET 0 25W 1 100PPM 108 R180 1 0 EA DALE CCF 55 4 1181 3500 216 RES FILM MET 0 254 1 100PPM 8258 R181 1 0 EA CORNING RN60D8250F 4 1182 3500 05 RES FILM 0 254 1 22 3 R182 1 0 EA DALE CCF 55 4 1183 3500 084 RFS FILM 0 254 1 100 22 1 R183 1 0 EA DALE CCF 55 4 1184 3500 009 RES FILM MET 0 254 1X 100PPM 10
61. M BLK S304 1 0 EA ELMA 040 1020 4 710 3310 018 KNOB CAP FOR 10MM BLK 305 1 0 ELMA 040 1020 4 711 3310 021 KNOB CAP FOR 14 5MM BLK S306 1 0 ELMA 040 3020 4 712 3310 021 KNOB CAP FOR 14 5MM BLK S314 1 0 EA ELMA 040 3020 4 713 3310 021 KNOB CAP FOR 14 5MM BLK S315 1 0 EA ELMA 040 3020 4 801 4010 001 DIODE LIGHT EMITTING CRM BEZ RED 05301 1 0 EA ARCO LH43V1301 A 4 802 4010 001 DIODE LIGHT EMITTING CRM BEZ RED 0 302 1 0 1 43 1301 6 803 4010 003 DIODE LIGHT EMITTING CRM BEZ 05503 1 0 EA ARCO LH43V1503 A 6 804 4010 003 DIODE LIGHT EMITTING CRM BEZ GRN 05304 1 0 ARCO LH43V1503 A 4 805 4010 002 DIODE LIGHT EMITTING CRM 2 YEL 05305 1 0 ARCO LH43V1401 A 4 806 4010 002 DIODE LIGHT EMITTING CRM BEZ YEL 05306 1 0 EA ARCO LH43V1401 A 4 807 4010 000 DIODE LIGHT EMITTING 1 RED DIFFUS 05307 1 0 H P HLMP 1301 4 851 4020 000 DIODE SI SIGNAL 1N914 D301 1 0 FAIRCHLD 1N914 4 852 4020 000 DIODE SI SIGNAL 1N914 0302 1 0 EA FAIRCHLD 11914 4 853 4020 000 DIODE SI SIGNAL 1N914 D303 1 0 EA FAIRCHLD 1N914 amp 854 4020 000 DIODE SI SIGNAL 18914 D304 1 0 EA FAIRCHLD 1N914 4 901 4510 000 METER PANEL 1 999V C INTENSITY 1 1 0 PM35U C 4 902 4510 000 METER PANEL 1 999V INTENSITY M2 1 0 TEXMATE PM35U C 4 903 4510 000 METER PANEL 1 999V C INTENSITY 5 1 0 PM35U C am
62. Mfg Manufacturer Part amp 18 3750 004 SWITCH TGL 1P ON OFF ON MIL S 83731 S318 1 0 MICROSWT 1TW1 7 4 19 3720 000 SWITCH PB MINI MOM 1P2T S319 1 0 EA 8121SY2GE 4 20 3750 004 SWITCH TGL 1P ON OFF ON MIL S 83731 S320 1 0 EA MICROSWT 1 1 7 6 52 3720 002 SWITCH CAP FOR MINI PB 0 375 BLACK S317 1 0 C amp K 7527 2 4 53 3720 002 SWITCH CAP FOR MINI PB 0 375 BLACK S319 1 0 7527 2 6 61 3720 009 SWITCH LENS FOR 308 1 0 UNIMAX THO1 982 G 6 62 3720 008 SWITCH LENS FOR RECT YEL 309 1 0 EA UNIMAX THO1 982 Y 4 63 3720 010 SWITCH LENS FOR RECT BLU 310 1 0 UNIMAX THO1 982 8 4 64 3720 007 SWITCH LENS FOR RECT PB RED 311 1 0 UNIMAX THO1 982 R 4 71 6300 000 A LABEL SET OF 4 AXOCLAMP 2 MODE SWITCHES 308 1 0 EA AXON 4 81 3720 006 LAMP INCAND 1 75 GRVD 14 4 0 EA LEDTRNCS 386 4 101 2300 111 B ELMCH THUMBWHEEL TO VOLTAGE CONVERTER A1004 1 0 EA y AXON 2297 v1 4420 004 3 5 DIGIT CMOS D A 7525K A1 1 0 EA ANLGDEVC AD7525KN 5 2 4200 000 IC LOW OFFSET LT1001 A2 1 0 LINTECH LT1001CN8 5 101 3500 003 RES FILM MET 0 25W 1X 100PPM 1008 R1 1 0 EA DALE 55 5 102 3500 015 RES FILM 0 25W 1 100PPM 1M R2 1 0 DALE CCF 55 5 103 3540 000 NETWORK 7 AT 10K 8 PINS RP1 1 0 BOURNS 4608 101 103 2 104 3540 000 NETWORK 7 AT 10K 8 PINS RP2 1 0
63. N 4 26 4200 018 IC FET 1556 A26 1 0 EA LINTECH LT1056CN8 6 25 4200 018 n AMP FET 1 356 i 25 1 0 LINTECH LT1056CN8 4 26 2200 018 IC AMP FET LF356 A25 1 0 EA LINTECH LT1056CN8 4 27 4200 018 IC AMP FET LF356 27 1 0 EA LINTECH LT1056CN8 2 28 5202 008 1 111056 A28 1 0 LINTECH 71056 6 2 z00 0C8 IC OP FET 111056 A29 1 3 LiNTECH LT1056CN8 24 3 4700 000 OP LOW OFFSET LT1001 A30 1 0 EA LINTECH 171001 8 24 31 4200 000 1C OP AMP LOW OFFSET LT1001 A3 1 0 LINTECH LT1001CN8 44 32 4200 001 IC OP AMP LOW NOISE LT1007 A32 1 0 EA LINTECH LY1007CN8 4 33 4260 008 IC AMP FET 111056 33 1 0 LINTECH 111056048 6 51 4100 008 CMOS PHASE LOCKED LOOP KATSEMI 4046 01 1 0 40068 2 4252 4100 003 CMOS S STAGE JOHNSON COUNTER 4017 U2 1 0 EA MOTOROLA MC14017BCP 4 53 4100 000 1C MOS QUAD 2 INF NOR 4001 1 0 FAIRCHLD 400182 54 4100 001 175 ENOR QUAD 2 NANG 4011 Us 1 9 EA MOTOROLA MC14i 1BCP 55 4100 009 IC CMOS HEX INVERTING BUFFER 4049 U5 1 6 MOTOROLA MC14049UBCP 4 56 4100 005 1C CMOS TRIPLE 3 NOR 4025 6 1 0 HARRIS CO4025BE 4 57 4100 005 CMOS TRIPLE 3 INP NOR 4025 07 1 0 HARRIS 04025 2 6 58 4100 006 IC CMOS 1 0 10 DECODER 4020 8 1 0 MOTOROLA
64. NA PACKAGING ZIP LOCK BAG 6 X 8 X 0 002 ELEC POWER CORD U S INTERNATIONAL CABLE LINE SHIELDED U S TO 2M CABLE LINE IEC TO CONTINENTAL EUROPEAN 2M CABLE LINE IEC TO AUSTRALIA 2M CABLE LINE IEC TO BRITISH 2M ELMCH FS 2 FOOT SWITCH SWITCH FOOT MOM 1P2T PLUG BANANA 4 44MM STACKING RED PLUG BANANA 4 44MM VIOLET CABLE LAMP 2 X 18G 41 X 34 BLACK NONE NONE NONE NONE NONE NONE NONE NONE NONE NONE NONE none GND LEAD P1 P2 NONE NONE NONE J1 J2 J3 NONE R1 R2 R3 c1 1 43 NONE NONE NONE NONE NONE 1 P1 P2 NONE O O o BP gt o o o ol A o We 2 I o eh oo aN gt AMPHENOL AMP AMP HEYCO ELMA ELMA AXON y AXON AXON BAYCITY LEDTRNCS BARKOFF AXON y AXON y AXON MULTCNTC MULTCNTC POMONA ICORALLY AXON AXON AXON CONCORD POMONA ELCTRNIC ELCTRNIC ELCTRNIC MALLORY C amp K CONCORD AXON AXON AXON BAYCITY BARKOFF AXON BELDEN PANLCOMP PANLCOMP PANLCOMP _ LINEMSTR EFJOHN EF JOHN BELDEN 9779 515 4 117 DA 15P DA 15 2034 7527 2 7527 BLU 021 2220 040 1020 2514 6X8ZIP2MIL LAMP ASSY 386 3X521PMIL CLAMP 1 MODEL CELL 22 10021 5
65. NN BNC ISOLATED PANEL MOUNT FEMALE 4407 1 0 EA 227726 2 S4 8 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE J408 1 0 EA 227726 2 4 9 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 4409 1 0 227726 2 4 10 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 2410 1 0 EA 227726 2 usr qad c p Page 15 Level Item Component 2 4 BILL OF MATERIAL BY ITEM REPORT Axon Instruments inc Description Qty UM P Mfg C 15 Date 02 13 90 Time 12 49 30 Manufacturer Part 101 102 202 203 204 205 206 212 213 214 215 216 301 5 ENSE eae se AERE dE CE PRRR dM dA GA RRR RRR de Ge dM dA dM GM dA RR d de d Ul gt 401 101 102 103 201 202 301 401 501 502 503 ES RoR gt Oo Ou f UNA 3250 004 3250 004 3250 004 3250 004 3250 004 3250 004 3250 004 3230 002 3230 004 3230 004 3230 000 3200 000 3240 005 3320 003 3320 003 3220 009 3220 010 3220 008 3220 010 3220 008 3220 017 3220 018 3220 016 3220 018 3220 016 2600 080 5300 016 5308 016 2300 054 5100 600 5100 005 5100 002 5540 005 5540 004 3810 000 3800 001 5100 010 5100 611 5020 001 2270 025 3120 001 3100 014 3160 001 3126 001 3160 014 3160 001 3100 014 3120 002 3120 002 3120 003 3100 021 3100 021 gt gt CONN BNC ISOLATED PAN
66. OIS KEMET PN2484 PN2484 PN2484 PN2484 PN2484 2N4036 2N700 CIL 254 CIL 254 CIL 254 CIL 254 184614 144614 144614 144614 MA78LOSAWC 1N914 1N914 14914 SXL310 C323C10425U5CAC924 C323C10425U5CAC924 PRI 103 K 2A PRI 332 K 2A PRI 102 K 2A PRI 103 K 2A PRI 332 K 2A GE151K T353E106K025AS 1353 106 025 5 PRI 222 K 2A GE100D GE100D GE330K GE150K GE151K T353E106K025AS T353E106K025AS PRI 102 K 2A PR1 102 K 2A GE330K GE 150K PRI 102 K 2A 1353A105K035AS C323C10425U5CAC924 107RMRO25 323C10425USCAC924 C 3 usr qad c p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 Page 3 Axon Instruments Inc Time 12 47 44 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part amp 430 3120 006 CAP ALUM ELCTLT RDL LEAD 25V 100U c30 1 0 EA ILLINOIS 107RMR025 4 431 3100 020 CER ML 50V 0 21 0 10 c31 1 0 323 10475 5 924 4 432 3100 020 CER ML 50V 0 2IN 0 1U 632 1 0 323 1042505 924 4 433 3100 020 CER ML 50V 0 21 0 10 C33 1 0 EA KEMET C323C10425U5CAC924 4 434 3160 000 CAP TANT 35V 10 C34 1 0 EA KEMET T353A105K035AS amp 435 3130 000 CAP FILM 10X 100V 0 001U C35 1 0 EA 1 102 2 4 436 3130 001 CAP FILM 10 100V 0 010 C36 1 0 EA ARCO PRI 103 K 2A amp 437 3100 001 CAP CER DISC 50V 10 100P 637 1 0 EA MALLORY GE101K 4 438 3160 000 CAP TANT
67. ONE 1 0 EA AXON 5 702 3435 004 C SCH THUMBWHEEL TO VOLTAGE CONVERTER NONE 1 0 AXON 4 201 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE J301 1 0 AMP 227726 2 4 202 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 4302 1 0 227726 2 4 203 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 4303 1 0 EA 227726 2 4 204 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 4504 1 0 227726 2 6 205 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE J305 1 0 AMP 227726 2 4 206 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 4306 1 0 EA 227726 2 4 301 3220 008 CONN INS DISPL SOCKET 0 100 GOLD 4 PIN P301 1 0 EA PANDUIT CE100F24 4 D A 6 302 3220 011 CONN INS DISPL SOCKET 0 100 GOLD 16 PIN P310 1 0 EA PANDUIT 100 24 16 4 303 3220 012 CONN INS DISPL SOCKET 0 100 GOLD 20 PIN P312 1 0 EA PANDUIT CE100F24 20C A 4 304 3220 012 CONN INS DISPL SOCKET 0 100 GOLD 20 P313 1 0 PANDUIT CE100F24 20C A 4 305 3220 011 CONN INS DISPL SOCKET 0 100 GOLD 16 PIN 314 1 0 PANDUIT CE100F24 16 T A 6 306 3220 009 CONN INS DISPL SOCKET 0 100 GOLD 8 PIN P315 1 0 EA PANDUIT CE100F24 8 D A 4 307 3220 008 CONN INS DISPL SOCKET 0 100 GOLD 4 1 P317 1 0 PANDUIT CE100F24 4 D A amp 310 3220 016 CONN COVER FOR 0 100 INS DISPL 4 PIN P301 1 0 PANDUIT 5 100 40 4 311 3220 019 CONN COVER FOR 0 100 INS DISPL 16 PIN P310 1 0 EA PANDUIT 5 1
68. P 7 3436 0020 NSCHNOUZ peer Tor 1 l V REVISION RECORD ADDED C82 AND R250 20 0 4 11 89 365 AQ OPAMP WAS 7101 PAGE 4 Fads OF 10 PER ECO 515 98 08 00 15V 15 10P 912 R215 191 AD7512 5682 10K 0 1 CONT 6190 10 10K 0 1 J402 CAL EXT STEP ACT 26 27 VCLAMP 27 Le R164 R165 10K 0 1 10K 0 1 R202 1008 J10 15 J2 1 5V REFERENCE m R 4 42 2 gt THUMB WHEEL OUTPUT 32 5 12 4 THUMBWHEEL pes ASSEMBLY A1004 42 5 212 15 gt 42 6 DESTINATION 15 posco SWITCH 12 7 C ow Joanna 63 02 89 INSTRUMENTS INC MARC 04 11 89 gt NEXT ASSY USED ME SCH 5V REF DESTINATION SWITCH 2270 024 AXOCLAMP 2A 5430 001 2 MAIN EC SIZE AXON PART NO REV 001 Plat 11 29 90 17 27 f ASCHYOOTA 10 Plotted 06 08 90 18 03 82 5 22 Cis 33p 111056 R68 15 1 RVTI3 15V ccs CLK RELAYS SHOWN INACTIVE VIA VCLAMP 26 R15 4 75 464 2 R44 2K74 R225 22R1 6 11007 L 9 REVISION RECORD DESCRIPTION OF CHANGE B SEE SHEET 1 252 Vot TP2 TO Im MONITOR CKT 23 14 2 8 15 e EE mn
69. RUMENTS INC MARC exin 6 11 8 22702074 00 2 UNLESS QTHERWESE AXON PART NO a 34 5 0 01 L o TOLEN 0027 Plotted 05 08 90 18 16 8 57 10 26 lU TANT ssy MODE SELECT SWITCHES J3 3 gt 04025 3 o 1 VOD 16 55 8 00 C002 1 11 12 70 E Fur M REMOTE 3424 31 5318 49 1 43 15 5v C 312 14 5 43 7 CLEAR Buzz 2 CLEAR FOOTSMTCH Plotai 06 08 90 18 18 a MODE SELECT RELAY DRIVE AND REMOTE CLEAR BUZZ CLOCK U5 4 210 BUZZ CLEAR CLEAR 8022 ME2 CLEAR ME2 CLEAR ME2 17 TURN 05307 312 20 5 5 8163 REVISION RECORD pesca Ton oF REV DAT CYCLE OFF ME1 SEVC R138 2281 i5 816 2281 4 13 14 12 A 054 TEVC TET A 053 SEVC A 052 DCC os BRIDGE SEVC 9 10 26 ME uec 2 SEVC 8 04025 11 ON 13 9 3 gt J1 4 FREQUENCY VOLTAGE COUNTER CIRCUITRY R238 100K 015 VFC32KN 15 R235 100K PARTS UST ow 03 02
70. S FILM 0 258 1 100PPM 100K R103 1 0 EA DALE 55 4 1104 3500 057 RES FILM 0 25W 1 100PPM 18K2 R104 1 0 EA CCF 55 4 1105 3500 159 RES FILM MET 0 25W 1 100PPM 4K75 R105 1 0 EA DALE CCF 55 4 1106 3500 066 RES FILM MET 0 254 1X 100PPM 2K R106 1 0 EA DALE CCF 55 amp 1107 3500 066 RES FILM MET 0 259 1 100PPM 2K R107 1 0 DALE CCF 55 4 1108 3500 081 RES FILM MET 0 250 1 100PPM 2K21 R108 1 0 EA DALE CCF 55 amp 1109 3500 012 RES FILM MET 0 254 1 100 100K R109 1 0 EA DALE CCF 55 4 1110 3500 219 RES FILM 0 254 1 100PPM 8K25 110 1 0 DALE 55 amp 1111 3560 001 RES FILM 0 25W 0 1 25PPM 10 00 R111 1 0 EA DALE CMF 55 T9 B amp 1112 3500 009 RES FILM MET 0 254 1X 100PPM 10K R112 1 0 EA DALE 55 6 1113 2560 001 RES FILM 0 259 0 1 25PPM 10 00 R113 1 0 EA DALE CMF 55 T9 B 6 1114 3560 001 RES FILM MET 0 254 0 1 25PPM 10 00 R114 1 0 EA DALE CMF 55 T9 B amp 1115 3500 171 RES FILM MET 0 254 1 100 499R R115 1 0 EA DALE CCF 55 4 1116 3560 001 RES FILM 0 25W 0 1 25 10 00 R116 1 0 DALE CMF 55 T9 B 4 1117 3500 180 RES FILM 0 254 1X 100PPM 499K R117 1 0 EA DALE CCF 55 amp 1118 3500 204 RES FILM MET 0 25W 1 100PPM 6K81 R118 1 0 EA DALE CCF 55 4 1119 3500 012 RES FILM MET 0 254 1X 100PPM 100K R119 1 0 DALE CCF 55 6
71. S FILM MET 0 254 1 100PPM 27 4 R151 1 0 EA DALE CCF 55 6 1152 3500 192 RES FILM 0 254 1 100PPM 56 2 R152 1 0 EA DALE CCF 55 4 1153 3500 006 RES FILM MET 0 25W 1 100PPM 1K R153 1 0 EA DALE CCF 55 2 4 1154 3500 141 RES 0 254 1 100 3928 2154 1 0 EA DALE 55 4 1155 3500 057 RES FILM 0 254 1X 100 18 2 R155 1 0 EA DALE 55 4 1156 3500 006 RES FILM 0 254 1 100PPM 1K R156 1 0 EA DALE CCF 55 4 1157 3500 204 RES FILM 0 25 1 100PPM 6k81 8157 1 0 EA DALE 55 2 4 1158 3500 006 RES FILM 0 25w 1 100PPM 1K R158 1 0 DALE 55 4 1159 3500 015 RES FILM MET 0 25W 1X 100PPM 1M R159 1 0 EA DALE CCF 55 4 1160 3500 015 RES FILM 0 25W 1 100PPM 1M R160 1 0 EA DALE 55 4 1161 3500 081 RES FILM 0 25 1 100PPM 2K21 R161 1 0 DALE CCF 55 4 1162 3500 066 RES 0 254 1 100 2 R162 1 0 DALE CCF 55 6 1163 3500 123 RES FILM 0 25W 1 100 3328 R163 1 0 DALE CCF 55 4 1164 3560 001 RES FILM 0 254 0 1 25PPM 10 00 1 0 DALE 55 9 4 1165 3560 001 RES FILM 0 254 0 1 25PPM 10 00 R165 1 0 DALE CMF 55 19 B 4 1166 3500 609 RES FILM 0 254 1 100PPM 10K R166 1 0 EA DALE 55 4 1167 3500 009 RES FILM 0 25W 1 100PPM 10K R167 1 0 EA DA
72. S INC Appendix 7 EQUIPMENT OSCILLOSCOPE UNDER TEST FIG 2 DIFFERENTIAL MEASUREMENTS ON OSCILLOSCOPE Correct technique for connecting an oscilloscope to make differential measurements in the frequency ranges encountered in the AXOCLAMP 2A AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC Appendix B C 1 usr qad c4 p 2 4 BILL OF MATERIAL BY ITEM REPORT Date 02 13 90 1 Axon Instruments Inc Time 12 47 23 Level Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part PARENT AXOCLAMP VOLTAGE CLAMP EA 1 101 2500 000 MANUAL 5 AXOCLAMP 2 1 0 2 1 6020 002 DOC COVER W WINOOW NAV BLU PRINTED NONE 1 0 EA GBC 5000052 2 2 6020 005 A BINDING SPIRAL 0 500 DIAMETER WITH NAME NONE 1 0 EA AXON 1 101 2950 034 A S U AXOCLAMP 1 0 AXOCLAMP 2 1 2300 047 ELMCH AXOCLAMP 1 0 3 101 2270 024 0 STUFFED AXOCLAMP 2 MAIN BOARD NONE 1 0 EA y AXON 4 1 4200 008 111056 1 1 0 EA LINTECH LT1056CN8 4 2 4220 008 IC VOLTAGE REF 5 02 A2 1 0 LINTECH REF O2CN8 4 3 4200 000 IC CP AMP LOW OFFSET LT1001 A3 1 0 EA LINTECH LT1001CN8 4 4 4200 001 IC OP AMP LOW NOISE LT1007 4 1 0 EA LINTECH 171007 8 4 5 4
73. ches Display selections are L and Currents exceeding the digital display range can be measured on the BNC outputs AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC SPECIFICATIONS 2 7 11 Outputs 10 and Im are membrane voltage gain 10 and current recorded by microelectrode 1 V and I are the continuous microelectrode 1 voltage and current V2 and L are microelectrode 2 voltage and current MONITOR is the output of the anti alias filter equals the input of the sampling device Gain 10 Baseline correction circuit automatically references Monitor trace to zero volts Gain of current outputs is 10 mV nA Maximum output level is 13V Current outputs indicate the true electrode current Output Lowpass Filter Cutoff 0 1 0 3 1 3 10 30 kHz Operates Vm and Im Single pole filter Output impedances are 500 0 10 VOLTAGE CLAMP 10 90 Rise Time following values were measured using 10 and nF in parallel to model the cell 10 resistors to model the microelectrodes and a 10 mV step command Rise Time in dSEVC mode 100 ys Rise Time in TEVC mode 30 ys Gain Maximum in dSEVC mode is 100 x H nA mV Maximum in cSEVC mode is 1000 x H nA mV Maximum in TEVC mode is 10 000 mV mV Range is 300 1 logarithmic scale Output compliance 25 V Phase Shift Time Constant ms 0 02 0 2 20 200 Lead range ms 0 0 0 04 0 0 4 0 4 0 40 0 40
74. e LIII ow joanna MARC PARON 04 11 89 11 89 P 5430 001 UMLESS OTHERWISE PARTS UST pg AXON INSTRUMENTS INC MME DRIVE 2 MAIN Scar 5 50018 2 or 10 10K 2008 06 08 90 18 05 221 0 10 CAL SV REF 21 REVISION RECORD oF E SEE SHEET 1 1 DISPLAY SELECT 28 9305 i PART NO RENAE OR seu ATURE OR ow Joanna H N o3 02 89 INSTRUMENTS INC PARON 04 11 69 le mm a 2270 024 Im MONITOR 3430 0091 2 UNLESS OTDENWSE 5 10 4 12 1 VT 21 1405 11 12 DC CURRENT 5V REF COMMAND R310 Plotted 06 08 90 18 08 REMSION RECORD PROVED REV are 464 2 3 6 2 5 62 ER NET 279 2K21 Q 30 CRDI 6 2 BUZZ CIL 252 ME2 4 5V 2229 R226 4K75 2281 1 3 105 E 15P PARTS UST jor om Joanna 63 02 89 INSTRUMENTS INC eren AM e AKON INSTRUMENTS INC ASSY USD ON CURRENT COMMAND amp 2270 024 3430 00
75. eld control OPTIONAL ACCESSORIES not required for normal operation 5 4 Relay Switched Headstage Miniature relay inside headstage automatically the current measuring resistor during two electrode voltage clamp mode In all other modes HS 4 headstage behaves like an HS 2MG headstage with Must be used in conjunction with a VG 2 virtual ground headstage VG 2 virtual ground headstage Measures total bath current The virtual ground output attenuation VG is available in three values specify with order x 0 1 x1 standard and x10 The output is 10 VG ORDERING INFORMATION When ordering please specify 1 Current gain H of two headstages provided 2 Gain and type of any extra headstages 3 Diameter D of headstage mounting rods Unless you specify otherwise the AXOCLAMP 2A will be supplied with one HS 2L x1 and one HS 2L x 0 1 headstage each with D 5 16 7 9 mm AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC Appendix 1 DRAWING CONVENTIONS SIGNAL IDENTIFICATION CAL refers to a signal called CAL going to ME2 the ME2 section of the circuit CAL refers to a signal called CAL coming from SV REF the SV REF section of the circuit SIGNAL NAMING The same name may be used for related devices signals and time periods Logic signals are named to correspond to positive logic Thus CYCLE ON is HI when the cycle is on and LO when the cycle
76. ents Axon Instruments continually tries to improve its products Because shipping and manual printing schedules sometimes conflict your manual may not contain the most up to date information The following pages contain change information unless your manual is correct as printed DR HARVEY J KARTEN M D UNIVERSITY OF CALIFORNIA SAN DIEGO DEPARTMENT OF NEUROSCIENCES 0608 9500 GILMAN DRIVE LA 92093 0608
77. escription Ref Qty UM P Mfg Manufacturer Part amp 1243 3500 075 RES FILM MET 0 25M 1 100 2281 R423 1 0 DALE CCF 55 amp 1243 3500 078 RES FILM MET 0 254 1 100PPM 221R R243 1 0 DALE CCF 55 amp 1244 3500 162 RES FILM MET 0 254 1 100PPM 47 5 R244 1 0 EA DALE 55 4 1245 3500 204 RES FILM 0 25W 1 6 81 R245 1 0 EA DALE CCF 55 6 1246 3500 156 RES FILM MET 0 254 1 100 4758 R246 1 0 EA CORNING RN60D4750F 6 1247 3500 012 RES FILM MET 0 254 1 100PPM 100K R247 1 0 EA DALE CCF 55 6 1248 3500 126 RES FILM 0 25W 1 100 3 32 R248 1 0 EA DALE CCF 55 4 1249 3530 001 RES HI MEG CARBON CMPSN 0 25W 5 10M R249 1 0 EA ALLNBRAD RCO7GF106J 4 1250 3500 078 RES FILM 0 254 1 100 221R R250 1 0 EA DALE CCF 55 4 1401 3800 002 TERMINAL PCB TEST EYELET 1 1 0 BICCVERO 20 21370 4 1402 3800 002 TERMINAL PCB TEST EYELET TP2 1 0 BICCVERO 20 2137D amp 1403 3800 002 TERMINAL PCB TEST EYELET TP3 1 0 EA BICCVERO 20 21370 amp 1404 3800 002 TERMINAL TEST EYELET 1 0 EA BICCVERO 20 21370 4 1405 3800 002 TERMINAL PCB TEST EYELET TP5 1 0 EA 20 21370 amp 1406 3800 002 TERMINAL PCB TEST EYELET TP6 1 0 EA BICCVERO 20 21370 4 1407 3800 002 TERMINAL PCB TEST EYELET 1 0 BICCVERO 20 21370 4 1408 3800 002 TERMINAL TEST EYELET TP8 1 0
78. for controlling the high speed switching between current passing and voltage recording U1 is a voltage controlled oscillator VCO whose frequency is set by the Rate control U2 is a Johnson counter which divides the VCO clock by ten and has an output cycle divided into ten segments U3 and U4 convert the ten segments into the required control signals see Fig 1 5 activates the main sample and hold SH1 in the section SH2 activates the sample and hold used for current measurement CCS switches the MEI constant current source from current passing to voltage recording In each cycle a sample of voltage is taken 1096 of cycle and then current passing begins 30 of cycle Shortly after current passing begins the sample and hold used for current measurement is activated 20 of cycle HI logic signal on the Blank Activate input prevents 1 from taking samples but does not otherwise affect the cycling U13 is a quad 2 input multiplexer used for synchronizing the clocks of two AXOCLAMPs Unless a second AXOCLAMP is connected all the switches in U13 remain in the positions shown U15 is a frequency to voltage converter It is used to drive the voltmeter which indicates the Sample rate Remote Clear Buzz Relays used for Buzz and Clear are driven by Darlington transistors in U10 transistor can be switched on by a front panel switch or by a nee HI signal applied through the remote connector or rear panel Buzz jacks AXO
79. headstage the input resistance of each headstage is measured unique test results are supplied with each x0 0001 headstage Input Capacitance s Not relevant See 1 settling time and noise specifications Input Leakage Current Adjustable to zero Input Leakage Current vs Temperature 10 fA x0 0001 100 fA 0 01 x0 1 1 10 10 Offset Neutralization Range 500 mV Ten turn potentiometers Capacitance Neutralization Range HS 2L 1 to 7 pF HS 2M 2 to 20 pF HS 2MG 4 to 18 pF These values apply when headstage is used with microelectrode 1 amplifier With microelectrode 2 amplifier the maximum values are doubled Buzz Instantly increases capacitance neutralization to cause oscillation Operated by spring loaded pushbutton switch footswitch or by Remote Buzz Duration control The latter allows the Buzz duration to be set in the range 1 50 ms Buzz Duration 1 50 ms when activated by the remote buzz control Clear Forces Imax through the microelectrode Spring loaded toggle switch Bridge Balance Range 10 H MO turn in Bridge mode 1 H MO turn in cSEVC mode Ten turn potentiometers Digital Voltmeters Voltage Displays 1999 Separate meters for and V2 Current Displays 19 99 pA H x 0 0001 1 999 nA 01 19 99 nA x 0 1 199 9 nA 1 1 999 mA x 10 Scaling is set by miniature panel swit
80. is off A bar over the name indicates the logic complement In the text the bar is indicated by underlining Thus SEVC is LO during SEVC mode and HI otherwise Signal names in square boxes appear on the front and rear panels COMPONENT VALUES Electrical components shown on the diagrams in the following units unless noted otherwise Capacitors Values one or greater are picofarads pF Values less than one are in microfarads uF Resistors Ohms SPECIAL SYMBOLS Jx y Indicates SIGNAL leaves circuit board at SIGNAL connector number x pin y Relays and semiconductor switches are normally shown unenergized L Trim potentiometer Panel potentiometer RESERVED WIRE COLORS Orange 5 15 Yellow Signal ground Blue 15 Digital ground AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 4538 16 GND28 TRIG CLR DURATION SINGLE TURN LOG TAPER R4 10K C 0 10 310 ML CERAMIC DATE PLOTTED 11 27 89 REVISION RECORD REV DESCRIPTION OF CHANGE DATE PART NUMBER WAS 0059 EXER 06 17 89 mrp INTERCONNECTING CABLE NOT A PART OF 0059 a own MARC PARON 04 27 86 AXON INSTRUMENTS INC ENG MARC 06 17 89 3 I EET NExT assy USED ON SCH REMOTE BUZZ 3430 002 AXOCLAM
81. oltage clamp output fed to during SEVC and ME2 during TEVC RL12 corrects the total gain during cSEVC Amplifier A23a buffers the input to A22 so that this signal can be observed by the experimenter on the Monitor output A23b subtracts the sampled potential so that the Monitor signal remains centered on OV Amplifier A20c detects the condition of zero output from the voltage clamp circuit This condition occurs when the Holding Position potentiometer balances V1A V1 Continuous The offset voltage generated in A20a is added to the buffered headstage voltage in A18a Bath potential is subtracted in A10b and the calibration signal is added AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC THEORY OF OPERATION Page 5 FIG 1 TIMING DIAGRAM DURING DCC and dSEVC MODES AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC Page 6 THEORY OF OPERATION This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC MAINTENANCE 7 MAINTENANCE Adjustment and repair should only be attempted by skilled electronic technicians or engineers CAUTION Line voltage is connected to some of the transformer leads and some parts of the power supply board in the left hand side of the instrument Always unplug the power cord before attempting to handle or repair these sections ACCESS All test points and trim potentiometers can be accessed by remo
82. own the microelectrode to maintain the cell membrane at the command voltage The required current is determined by a negative feedback circuit 1 TEVC mode two electrode voltage clamp is used for conventional voltage clamping is used to record membrane potential Vm is used to pass the required current dSEVC mode discontinuous single electrode voltage clamp uses MEI for both voltage recording and current passing required input of the negative feedback circuit is Vm Because of the large resistance of normal intracellular electrodes Vm cannot be measured during current passing Therefore the current in is chopped by high speed switching circuitry and the voltage is sampled between current pulses can be independently used as in Bridge mode cSEVC mode continuous single electrode voltage clamp Like dSEVC this mode uses for both voltage recording and current passing during voltage clamp Unlike dSEVC in this mode current passing is continuous and thus th voltage drop in the electrode due to current flow is measured This voltage drop introduces an error cSEVC only works with very low resistance suction electrodes so that the error is small Bridge potentiometer allows some of this voltage drop to be compensated Command voltages for both current passing and voltage clamping are generated internally but timing for these commands must come from external sources Fo
83. p 904 4510 000 METER PANEL 1 999V C INTENSITY M4 1 0 TEXMATE PM35U C 4 1001 3130 000 CAP FILM 10 1007 0 0010 301 1 0 EA ARCO PRI 102 K 2A 6 1002 3130 001 CAP FILM 10 100V 0 010 c302 1 0 EA ARCO PR1 103 K 2A 4 1003 3130 002 CAP FILM 10 1007 0 10 C303 1 0 EA ARCO PRI 104 K 2A amp 1004 3100 021 CAP CER ML 50V 20 1UF C304 1 0 KEMET 330 105 505 4 1005 3120 000 CAP ALUM BIPOLAR 25V 10U C305 1 0 EA SPRAGUE 1201 1 4 1006 3130 000 CAP FILM 10 100v 0 0010 C306 1 0 EA ARCO PRI 102 K 2A 4 1102 2600 022 MECH PANEL FRONT COMPLETE AXOCLAMP NONE 1 0 EA 5 101 5300 080 PANEL FRONT LEXAN AXOCLAMP NONE 1 0 EA y AXON 5 102 5300 081 A PANEL FRONT SUB PANEL AXOCLAMP NONE 1 0 EA y AXON 5 103 5305 080 A PANEL ARTWORK FRONT LEXAN AXOCLAMP NONE 1 0 3 301 2300 024 B ELMCH REAR PANEL AXOCLAMP 2 RONE 1 0 EA y AXON 4 1 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE J401 1 0 AMP 227726 2 4 2 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE J402 1 0 AMP 227726 2 4 3 3250 004 CONN ISOLATED PANEL MOUNT FEMALE 4403 1 0 227726 2 4 4 3250 004 CONN BNC ISOLATED PANEL MOUNT FEMALE 4404 1 0 227726 2 4 5 3250 004 b CONN BNC ISOLATED PANEL MOUNT FEMALE 4405 1 0 227726 2 4 6 3250 004 CONN ISOLATED PANEL MOUNT FEMALE 4406 1 0 227726 2 4 7 5250 00 CO
84. r a complete description of the operation of the AXOCLAMP 2A see the Theory and Operation manual POWER SUPPLY ASSEMBLY No A1001 Located in the left hand section of the instrument WARNING Line voltages are present in the power supply section The line input connector contains an RFI filter the fuse in use and a spare fuse A slide switch on the circuit board puts the two primary windings into series parallel connection for 230 V or 115 V operation respectively Line voltages from 200 260 V and 100 130 are acceptable AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 2 0 Five regulated outputs are generated 30 V 30 V 15 V 15 V 5 V Standard 3 terminal regulators are used Test points on the circuit board allow the regulator input and output voltages to be conveniently measured 2 mV of line frequency noise and 2 mV of wideband noise are acceptable This noise is rejected by the rest of the circuitry REMOTE INPUT AND CONTROL LOGIC ASSEMBLY No A1002 Power Distribution All power for the main circuit board comes from the power supply board to connector J8 Mode Select All switching between modes is done on the main circuit board by relays U6 U7 and U8 are used to decode the Mode Select switches U9 contains 7 Darlington transistors for driving relays and the Mode lamps Clock Sequence Generator 01 05 are used to generate the logic signals
85. sonen lt gt lt lt SWITCH JFET SPDT 2 FOR NEW DESIGNS IC CMOS QUAD 2 INP MULTIPLEXER 4019 COMPONENT NOT PRESENT IC V F F V CONVERTOR VFC32K TRANSISTOR NPN TO 92 2484 PN100A TRANSISTOR NPN TO 92 2484 PN100A TRANSISTOR NPN TO 92 2484 PN100A TRANSISTOR NPN TO 92 2484 PN100A TRANSISTOR NPN TO 92 2484 PN100A TRANSISTOR POWER PNP TO 39 2N4036 TRANSISTOR N MOSFET 0 92 2N7000 DIODE CUR RGLTR 600MW 60 7 5 11254 55 DIODE CUR RGLTR 600MW 60 7 5 CIL254 55 DIODE CUR RGLTR 600MW 60POV 7 5MA 11254 55 DIODE CUR RGLTR 600MW 60POV 7 5MA 11254 55 DIODE ZENER SOOMW 1 8V 1N4614 184678 DIODE ZENER SOOMW 1 8V 1N4614 184678 DIODE ZENER SOOMW 1 8V 1N4614 144678 DIODE ZENER 500 1 8V 1N4614 144678 IC VOLTAGE REG 5 78105 DIODE SI SIGNAL 1N914 DIODE SI SIGNAL 1N914 DIODE SI SIGNAL 1N914 CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP CAP FILM PS 50V 2 5 100P CER ML 50V 0 21 0 1U CER ML 50V 0 21N 0 1U FILM 10 100V 0 010 FILM 10 100V 0 0033U FILM 10X 100V 0 0010 FILM 10 100V 0 01U FILM 10 100V 0 0033U CER DISC 50V 10X 150P TANT 25V 10U TANT 25V 10U FILM 10 100V 0 00220 CER DISC 50V 10 10P CER DISC 50V 10 10P CER DISC 50V 10X 33P CER DISC 50
86. stage Drive Circuit 2 S A 6 EMT 7 Bridge Monitor V1 Continuous 8 Voltage Clamp amp VI oe aao 9 I Display Select V Bath Virtual 10 R mote Input amp sana Clock and Control Logic MEA RARIOR Nea A 12 Headstage and Model Cell Configuration cessere E 13 Power Supply Axoclamp ee 14 Thumbwheel to Voltage basa ee SR 15 AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC viii This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC THEORY OF OPERATION Page 1 THEORY OF OPERATION GENERAL The AXOCLAMP 2A contains amplifiers for 2 separate microelectrodes and ME2 In Bridge mode both microelectrodes are used for continuous current passing and voltage recording In DCC mode discontinuous current clamp the current in is chopped by high speed switching circuitry and the voltage on MEI is sampled between current pulses is used for continuous current passing There are three voltage clamp modesDuring voltage clamp current is passed d
87. t for about 50 kHz on frequency meter Trim RVT25 for same reading on panel counter AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 2 Appendix 2 Thumbwheel to voltage offset Bridge mode On scope measure TP7 VT TP8 gnd at 100 pV div Externally activate thumbwheel at 100 Hz Destination ME1 Thumbwheel on 000 0 Adjust trim pot on back of thumbwheel for a null 3 SV REF accuracy Bridge mode Using the DVM monitor TP7 VT Thumbwheel on 100 0 continuous Repetitively switch from 100 0 to 100 0 Trim for 10 000 V difference 4 51 CMRR Thumbwheel on 000 0 external MEI current clamp command off Plug in x1 headstage Connect a 1 Vp p 100 Hz square wave directly to the headstage input On scope measure 2 1 source voltage at 100 Trim RVTIO for a null 5 Im CMRR On scope measure TP11 Im TP8 gnd at 100 Trim RVTS for a null 6 MEI COM gain Remove headstage Link pins 8 gnd and 1 with 22 0 resistor Using the DVM monitor 2 1 Thumbwheel on 100 0 continuous Repetitively switch from 100 0 to 100 0 DCC mode 1 kHz Trim RVT22 for 2 000 difference Bridge mode Trim RVT12 for 2 000 difference 7 Im DPM accuracy Bridge mode Thumbwheel on 100 0 continuous Repetitively switch from 100 0 to 100 0 Adjust trim pot inside Im DPM to read 1 000 AXOCLAMP SERVICE COPYRIG
88. vel Item Component Rev Description Ref Qty UM P Mfg Manufacturer Part 4 1087 3560 001 RES FILM MET 0 25W 0 1 25 10 00 R87 1 0 EA DALE CMF 55 T9 B 4 1088 3500 084 RES FILM MET 0 250 1X 100PPM 22 1 R88 1 0 DALE 55 4 1089 3500 002 RES FILM 0 25W 1 100 108 R89 1 0 EA DALE CCF 55 6 1090 3500 069 RES FILM MET 0 25W 1 100PPM 20K R90 1 0 EA DALE CCF 55 4 1091 3500 069 RES FILM 0 25W 1 100PPM 20K R91 1 0 DALE CCF 55 4 1092 3500 069 RES FILM 0 25W 1 100PPM 20K R92 1 0 DALE CCF 55 4 1093 3500 069 RES FILM 0 254 1X 100PPM 20K R93 1 0 EA DALE 55 6 1094 3500 006 RES FILM 0 254 1X 100PPM 1K R94 1 0 EA DALE CCF 55 4 1095 3500 144 RES FILM MET 0 25W 1 100PPM 3 92 R95 1 0 EA DALE CCF 55 4 1096 3500 012 RES FILM 0 25W 1 100PPM 100K R96 1 0 DALE CCF 55 4 1097 3500 012 RES FILM MET 0 25W 1 100PPM 100K R97 1 0 DALE CCF 55 amp 1098 3500 099 RES FILM 0 25W 1X 100 27 4 R98 1 0 DALE CCF 55 amp 1099 3500 099 RES FILM 0 25W 1X 100PPM 27K4 R99 1 0 DALE 55 4 1100 3500 039 RES FILM MET 0 25W 1 100PPM 1K5 R100 1 0 EA DALE CCF 55 4 1101 3500 039 RES FILM 0 25W 1 100 1 5 R101 1 0 EA DALE 55 4 1102 3500 012 RES FILM 0 259 1 100K 102 1 0 EA DALE CCF 55 4 1103 3500 012 RE
89. ving the top cover All components can be desoldered from the main circuit board without removing the board Simply remove the bottom cover for access to the non component side of the board ROUTINE MAINTENANCE Routine maintenance is not required The adjustment procedure should be performed after repairs to the main circuit board but not otherwise As required the operator of the instrument can perform the headstage leakage current adjustment described in the operator s manual AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC 8 MAINTENANCE This page is intentionally left blank AXOCLAMP SERVICE COPYRIGHT FEBRUARY 1990 AXON INSTRUMENTS INC SPECIFICATIONS Page 9 SPECIFICATIONS MODES Five main operating modes selectable by color coded illuminated push buttons or remotely These are 1 Bridge 2 DCC Discontinuous Current Clamp 3 dSEVC Discontinuous Single Electrode Voltage Clamp 4 cSEVC Continuous Single Electrode Voltage Clamp 5 TEVC Two Electrode Voltage Clamp MICROELECTRODE AMPLIFIERS Two Channels Unity Gain Headstages Hum line frequency pickup Headstage Current Gain H Maximum Current Noise with grounded input Noise with a source resistance 1 Settling Time Standard is the HS 2L type 2 types are the same except 1 the noise is greater by about 20 2 the capacitance neutralization range is extended HS 2MG types are similar to the HS 2M types

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